Web-based CRISPR multiplex gene editing tool for designing Polycistronic tRNA-gRNA (PTG) assemblies using Golden Gate cloning strategy. Up to 12 spacers, 12 Type IIS enzymes, native and engineered gRNA scaffolds. Developed by ICAR-IARI and ICAR-IASRI.
php bioinformatics crispr crispr-cas9 grna primer-design gene-editing ptg plant-biotechnology golden-gate-assembly multiplex-genome-editing
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Updated
Sep 10, 2026 - PHP