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Gene list: add vfb-scRNAseq's FBgn list; give every expressed gene a class - #45

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Clare72 merged 1 commit into
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vfb-scrnaseq-gene-list
Oct 22, 2026
Merged

Clare72 merged 1 commit into
mainfrom
vfb-scrnaseq-gene-list

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@Clare72

@Clare72 Clare72 commented Oct 22, 2026

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scRNAseq data in VFB is moving from the old FlyBase/SCEA loads (vfb-scRNAseq-ontology) to vfb-scRNAseq, which publishes expression/FBgn_list.txt (VirtualFlyBrain/vfb-scRNAseq#100, merged).

Changes

  • The gene list now includes vfb-scRNAseq's list. The old scRNAseq list and the EPseq list stay until the old loads are retired, so both the old and new files are covered.
  • Every gene in the list now gets a Gene class (process_FBgn.py adds the listed genes to those from fbgn_fbtr_fbpp). 31 current genes that vfb-scRNAseq expresses are not Gene classes in VFB production: mostly non-coding CR… genes, plus CG42778 and pog. Without a class, their expresses edges would point at genes VFB doesn't have.

Not run locally: the build needs the manually downloaded FlyBase files described in the Makefile.

🤖 Generated with Claude Code

…class

scRNAseq is moving from the old FlyBase/SCEA loads (vfb-scRNAseq-ontology)
to vfb-scRNAseq. Its expression/FBgn_list.txt is added to the gene list
(the old list kept until the old loads are retired), and every gene in the
list now gets a Gene class: 31 current genes vfb-scRNAseq expresses (e.g.
non-coding CR genes) are missing from fbgn_fbtr_fbpp, so their expression
edges would point at genes VFB does not have.

Co-Authored-By: Claude Opus 5.5 <[email protected]>
@Clare72

Clare72 commented Oct 22, 2026

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Blocked for now: VirtualFlyBrain/vfb-scRNAseq is private, so the anonymous wget of expression/FBgn_list.txt from raw.githubusercontent.com returns 404. Merge this once that repo is public, or switch the download to an authenticated one.

@Clare72

Clare72 commented Oct 22, 2026

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Unblocked: VirtualFlyBrain/vfb-scRNAseq is now public, and https://raw.githubusercontent.com/VirtualFlyBrain/vfb-scRNAseq/main/expression/FBgn_list.txt returns 200. It now includes the Ing-Simmons fix (VirtualFlyBrain/vfb-scRNAseq#101): genes only, 15,516 FBgns.

@Clare72
Clare72 merged commit 470ea4f into main Oct 22, 2026
@Clare72
Clare72 deleted the vfb-scrnaseq-gene-list branch October 22, 2026 13:11
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