From b7fe6ffb10243f4fc908147131c7656d4499f190 Mon Sep 17 00:00:00 2001 From: Jason Stajich Date: Fri, 30 Oct 2026 06:08:28 -0700 Subject: [PATCH 1/5] db: MATss family and two S. schoenii candidate records (own tier-3 gene models, table 12); tests Co-Authored-By: Claude Sonnet 5.5 --- db/Ascomycota/order.yml | 37 + .../44080_gdd12_MATss_MATa/metadata.yaml | 127 + .../44080_gdd12_MATss_MATalpha/metadata.yaml | 144 + results/2026-10-29_ss_records/.gitignore | 5 + results/2026-10-29_ss_records/README.md | 27 + results/2026-10-29_ss_records/chr4_models.py | 39 + results/2026-10-29_ss_records/chr4_orfs.py | 55 + .../data/chr4_models.txt | 65 + .../2026-10-29_ss_records/data/chr4_orfs.tsv | 54 + .../data/depth_chrIV_455k-545k.tsv.gz | Bin 0 -> 135551 bytes .../2026-10-29_ss_records/data/genome.fa.fai | 7 + .../data/junctions_chrIV.tsv | 6 + .../data/miniprot_all.gff | 294 + .../2026-10-29_ss_records/data/stringtie.gtf | 7291 +++++++++++++++++ results/2026-10-29_ss_records/make_refs.sh | 6 + results/2026-10-29_ss_records/refs.faa | 126 + results/2026-10-29_ss_records/ss_models.slurm | 15 + results/2026-10-29_ss_records/ss_rnaseq.slurm | 29 + .../44080_gdd12_MATss_MATa.yaml | 126 + .../44080_gdd12_MATss_MATalpha.yaml | 144 + results/2026-10-30_saccharomycopsis/README.md | 15 + .../chrIV_463k-542k.fa | 989 +++ .../make_records.py | 90 + .../validate.slurm | 11 + tests/detect/test_fallback_searchable.py | 2 +- tests/detect/test_matss_family.py | 41 + 26 files changed, 9744 insertions(+), 1 deletion(-) create mode 100644 db/candidates/Ascomycota/44080_gdd12_MATss_MATa/metadata.yaml create mode 100644 db/candidates/Ascomycota/44080_gdd12_MATss_MATalpha/metadata.yaml create mode 100644 results/2026-10-29_ss_records/.gitignore create mode 100644 results/2026-10-29_ss_records/README.md create mode 100644 results/2026-10-29_ss_records/chr4_models.py create mode 100644 results/2026-10-29_ss_records/chr4_orfs.py create mode 100644 results/2026-10-29_ss_records/data/chr4_models.txt create mode 100644 results/2026-10-29_ss_records/data/chr4_orfs.tsv create mode 100644 results/2026-10-29_ss_records/data/depth_chrIV_455k-545k.tsv.gz create mode 100644 results/2026-10-29_ss_records/data/genome.fa.fai create mode 100644 results/2026-10-29_ss_records/data/junctions_chrIV.tsv create mode 100644 results/2026-10-29_ss_records/data/miniprot_all.gff create mode 100644 results/2026-10-29_ss_records/data/stringtie.gtf create mode 100644 results/2026-10-29_ss_records/make_refs.sh create mode 100644 results/2026-10-29_ss_records/refs.faa create mode 100644 results/2026-10-29_ss_records/ss_models.slurm create mode 100644 results/2026-10-29_ss_records/ss_rnaseq.slurm create mode 100644 results/2026-10-30_saccharomycopsis/44080_gdd12_MATss_MATa.yaml create mode 100644 results/2026-10-30_saccharomycopsis/44080_gdd12_MATss_MATalpha.yaml create mode 100644 results/2026-10-30_saccharomycopsis/README.md create mode 100644 results/2026-10-30_saccharomycopsis/chrIV_463k-542k.fa create mode 100644 results/2026-10-30_saccharomycopsis/make_records.py create mode 100644 results/2026-10-30_saccharomycopsis/validate.slurm create mode 100644 tests/detect/test_matss_family.py diff --git a/db/Ascomycota/order.yml b/db/Ascomycota/order.yml index 88c7bfff..5a0ce7e0 100644 --- a/db/Ascomycota/order.yml +++ b/db/Ascomycota/order.yml @@ -792,3 +792,40 @@ loci: # sla2: sla2_homolog by precedent; optional so that contigs ending inside the locus (MH260578 is one) are not demoted. - {name: sla2, role: flanking_conserved, gene_class: sla2_homolog, optional: true} - {name: tgl1, role: flanking_conserved, optional: true} + - locus_name: "MATss" + vocabulary_type: "enum" + # NEW FAMILY, curator decision 2026-10-30 (candidate records for the SLA2-type genera, results/2026-10-29_near_tie/ + # CANDIDATE_RECORDS.md): Saccharomycopsis (Ascoideales, Saccharomycopsidaceae) has its own scope-only family, named MATss + # after the genus as MATkp, MATog and MATpk are named after theirs; the code is the curator's to change. + # ARCHITECTURE (Kriti et al., G3 2026, PMID 41849659, doi 10.1093/g3journal/jkag067; Dost et al., Microbiol Res 2024, PMID 38492364): + # S. schoenii is HOMOTHALLIC and carries an expanded MAT system, SLA2-MAT cassettes (MATa1, MATa2, MATalpha1, MATalpha2) on every + # chromosome, with SLA2 as the anchor and the MAT genes co-transcribed with it (189-247 bp splice junctions between the MAT genes + # and SLA2). DIC1 is scattered, not linked. The active locus is on chromosome IV: an alpha-dominant sub-cluster (470,610-475,000) + # and a palindromic cassette (528,044-537,777, an SLA2 anchor flanked by mirrored MATa1/MATa2 arrays). Neither MATsc (three + # cassettes, HO switching) nor MTL (PAP1/OBP1/PIK1 inside the idiomorph) describes it: the replay of v0.7.0 called these loci MTL + # in 22 of 27 cases with SLA2 inside the call and no PIK1/OBP1/PAP1 (results/2026-10-29_near_tie/). + # `cassette_report` stays off: which cassette is expressed is not determined and one genome holds several per idiomorph. + # `two_idiomorphs_report` stays off (a homothallic species carries both idiomorphs by design). + idiomorph_values: ["MATa", "MATalpha"] + idiomorph_class: {MATalpha: unassigned, MATa: unassigned} + # SCOPE = THE GENUS Saccharomycopsis (taxid 4943; NCBI Taxonomy efetch 2026-10-30: rank genus, family Saccharomycopsidaceae 34366, + # order Ascoideales 2926619). Only S. schoenii has a record. Dost et al. 2024 describe S. lassenensis as heterothallic, so the genus is + # not uniform; the scope is the genus because 22 of the 27 loci the replay found in the genus have the SLA2-inside architecture, + # and it is the curator's to narrow to the species (44080) if a replay over the six Saccharomycopsis species over-calls. + taxonomic_scope: [4943] + fallback_subphylum: 147537 # Saccharomycotina + fallback_searchable: false + flank_carried_window_bp: 20000 + genes: + # Gene models of the records are OUR OWN (tier 3): the authors' GTF is not public (results/2026-10-29_ss_records/README.md). + # MATa1, MATalpha2: homeodomain; no class (the schema has none). Named after the paper's cassette (a: MATa1; alpha: MATalpha2); phmmer + # ranks both ORFs closest to MATa1-type references, so the name of the left-cluster homeodomain gene is a hypothesis. + - {name: MATa1, role: core_MAT, present_in_idiomorphs: ["MATa"]} + # MATa2: optional; no HMG-box ORF was resolved in the right cassette (an HMG-like ORF 470,349-470,951 is not assigned). + - {name: MATa2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATa"]} + # MATalpha1: alpha_box (Pfam PF04769 54 bits, GA 28.1, on the chromosome IV ORF), the same bar as MATkp, MATog and MATpk. + - {name: MATalpha1, role: core_MAT, gene_class: alpha_box, present_in_idiomorphs: ["MATalpha"]} + - {name: MATalpha2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATalpha"]} + # sla2: the anchor of every cassette; sla2_homolog by precedent. Optional so that truncated copies are not demoted. + - {name: sla2, role: flanking_conserved, gene_class: sla2_homolog, optional: true} + - {name: dic1, role: flanking_conserved, optional: true} diff --git a/db/candidates/Ascomycota/44080_gdd12_MATss_MATa/metadata.yaml b/db/candidates/Ascomycota/44080_gdd12_MATss_MATa/metadata.yaml new file mode 100644 index 00000000..0a86fe70 --- /dev/null +++ b/db/candidates/Ascomycota/44080_gdd12_MATss_MATa/metadata.yaml @@ -0,0 +1,127 @@ +record_id: 44080_gdd12_MATss_MATa +record_version: 1 +taxonomy: + taxid: 44080 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Ascoideales;f__Saccharomycopsidaceae;g__Saccharomycopsis;s__Saccharomycopsis_schoenii + lineage_resolved_date: '2026-10-30' +organism: + species: Saccharomycopsis schoenii + strain: + name: GDD12 + known: true + culture_collection_ids: [] + differs_from_sequenced: false +mating_type: + locus_name: MATss + idiomorphs: + - MATa + system: homothallic +locus: + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + core: + completeness: partial + reference_orientation: sla2->MATa1 + definition_note: 'Right (palindromic) cassette of the active MAT locus on chromosome + IV of S. schoenii GDD12 (CM143129.1; the paper''s span 528,044-537,777; an SLA2 + anchor between mirrored MAT arrays, Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC + (Dost et al. 2024, PMID 38492364). The record covers the arm that RNA-seq resolves: + SLA2 533,981-535,828 (plus, 615 aa, 5-prime partial: no start codon in the ORF) + and the homeodomain gene named MATa1 after the paper at 536,046-536,944 (minus, + 211 aa; introns of 205 bp, 221 reads, and 58 bp, 218 reads, the conserved 58 + bp junction the paper reports at 536,635). The mirrored arm (SLA2 529,992-532,490; + homeodomain ORF 529,258-529,749, 163 aa) is not recorded: short reads cannot + separate the two inverted copies, and the paper''s mirrored junction at 529,128 + is not seen. No HMG-box (MATa2) ORF passed the filters. GENE MODELS ARE OURS + (tier 3), see results/2026-10-29_ss_records/README.md. The library is not stranded. + GENETIC CODE: NCBI table 12 (CUG = Ser).' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: CM143129.1 + seq_region: CM143129.1 + start: 533981 + end: 536944 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] + assembly_accession: GCA_054953955.1 + assembly_accession_basis: strain_match_verified + assembly_location: + contig: CM143129.1 + start: 533981 + end: 536944 + identity: 100.0 +genes: +- gene_index: 0 + name: sla2 + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 533981 + end: 535828 + strand: + + order_in_locus: 0 + transl_table: 12 + exons: + - start: 533981 + end: 535828 +- gene_index: 1 + name: MATa1 + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 536046 + end: 536944 + strand: '-' + order_in_locus: 1 + transl_table: 12 + exons: + - start: 536693 + end: 536944 + - start: 536296 + end: 536634 + - start: 536046 + end: 536090 +evidence: + locus_existence: + tier: 2 + experimental_method: published description of the SLA2-MAT cassettes and RNA-seq + of the active chromosome IV locus (Kriti et al. 2026) + citations: &id001 + - pmid: '41849659' + doi: 10.1093/g3journal/jkag067 + - pmid: '38492364' + doi: 10.1016/j.micres.2024.127691 + boundaries: + tier: 3 + experimental_method: OUR OWN gene models from the public RNA-seq (PRJNA1364152; + StringTie, TransDecoder, phmmer/Pfam) because the authors' GTF is not public; + coordinates are ours, the locus span is the paper's + citations: *id001 + idiomorph_assignment: + tier: 3 + experimental_method: the paper's assignment of the sub-cluster (alpha-dominant + left, palindromic a right); gene names follow it by position; phmmer ranks both + homeodomain ORFs closest to MATa1-type references, so MATalpha2 is a hypothesis + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: '' + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: 41849659|44080|MATss|MATa + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/db/candidates/Ascomycota/44080_gdd12_MATss_MATalpha/metadata.yaml b/db/candidates/Ascomycota/44080_gdd12_MATss_MATalpha/metadata.yaml new file mode 100644 index 00000000..cb82ec1f --- /dev/null +++ b/db/candidates/Ascomycota/44080_gdd12_MATss_MATalpha/metadata.yaml @@ -0,0 +1,144 @@ +record_id: 44080_gdd12_MATss_MATalpha +record_version: 1 +taxonomy: + taxid: 44080 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Ascoideales;f__Saccharomycopsidaceae;g__Saccharomycopsis;s__Saccharomycopsis_schoenii + lineage_resolved_date: '2026-10-30' +organism: + species: Saccharomycopsis schoenii + strain: + name: GDD12 + known: true + culture_collection_ids: [] + differs_from_sequenced: false +mating_type: + locus_name: MATss + idiomorphs: + - MATalpha + system: homothallic +locus: + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + core: + completeness: partial + reference_orientation: MATalpha1->MATalpha2->sla2 + definition_note: 'Left (alpha-dominant) sub-cluster of the active MAT locus on + chromosome IV of S. schoenii GDD12 (CM143129.1; the paper''s span 470,610-475,000, + Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC (Dost et al. 2024, PMID 38492364); + one of several SLA2-MAT cassettes (one per chromosome plus a palindromic one + on chromosome IV). GENE MODELS ARE OURS (tier 3): the BRAKER2 GTF the paper + cites is not on its GitHub or Zenodo, so the models were rebuilt from the three + public paired RNA-seq runs (PRJNA1364152; hisat2, StringTie, TransDecoder, phmmer + against the curated MAT proteins, Pfam-A); see results/2026-10-29_ss_records/README.md. + The library is not stranded; strand is the ORF''s. MATalpha1 is single-exon, + minus strand, 191 aa. The homeodomain ORF (named MATalpha2 after the paper) + is partial (103 aa, two exons with a 217 bp intron of 198 reads that joins it + toward SLA2, the paper''s MAT-SLA2 splice junction); phmmer ranks it closest + to MATa1-type references (50 bits to C. albicans MTLA1), so the name is a hypothesis. + SLA2 here is a diverged copy: 256 aa (472,708-473,478, minus), 5-prime partial + (the ORF has no start codon); a second SLA2 piece (473,680-474,606, 309 aa) + is not recorded. An HMG-like ORF at 470,349-470,951 (200 aa, 44 bits to S. cerevisiae + MATA2, no Pfam hit) is not assigned to a gene. GENETIC CODE: NCBI table 12 (CUG + = Ser) for the species; proteins are translated with it.' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: CM143129.1 + seq_region: CM143129.1 + start: 471020 + end: 473478 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] + assembly_accession: GCA_054953955.1 + assembly_accession_basis: strain_match_verified + assembly_location: + contig: CM143129.1 + start: 471020 + end: 473478 + identity: 100.0 +genes: +- gene_index: 0 + name: MATalpha1 + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 471020 + end: 471595 + strand: '-' + order_in_locus: 0 + transl_table: 12 + exons: + - start: 471020 + end: 471595 +- gene_index: 1 + name: MATalpha2 + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 471961 + end: 472489 + strand: + + order_in_locus: 1 + transl_table: 12 + exons: + - start: 471961 + end: 472227 + - start: 472445 + end: 472489 +- gene_index: 2 + name: sla2 + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 472708 + end: 473478 + strand: '-' + order_in_locus: 2 + transl_table: 12 + exons: + - start: 472708 + end: 473478 +evidence: + locus_existence: + tier: 2 + experimental_method: published description of the SLA2-MAT cassettes and RNA-seq + of the active chromosome IV locus (Kriti et al. 2026) + citations: &id001 + - pmid: '41849659' + doi: 10.1093/g3journal/jkag067 + - pmid: '38492364' + doi: 10.1016/j.micres.2024.127691 + boundaries: + tier: 3 + experimental_method: OUR OWN gene models from the public RNA-seq (PRJNA1364152; + StringTie, TransDecoder, phmmer/Pfam) because the authors' GTF is not public; + coordinates are ours, the locus span is the paper's + citations: *id001 + idiomorph_assignment: + tier: 3 + experimental_method: the paper's assignment of the sub-cluster (alpha-dominant + left, palindromic a right); gene names follow it by position; phmmer ranks both + homeodomain ORFs closest to MATa1-type references, so MATalpha2 is a hypothesis + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: '' + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: 41849659|44080|MATss|MATalpha + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/results/2026-10-29_ss_records/.gitignore b/results/2026-10-29_ss_records/.gitignore new file mode 100644 index 00000000..81f01bb3 --- /dev/null +++ b/results/2026-10-29_ss_records/.gitignore @@ -0,0 +1,5 @@ +data/spliced_*.txt +data/transcripts.fa +data/transcripts.fa.transdecoder.* +data/longest_orfs.pep +refs.faa.bak diff --git a/results/2026-10-29_ss_records/README.md b/results/2026-10-29_ss_records/README.md new file mode 100644 index 00000000..f46d05e7 --- /dev/null +++ b/results/2026-10-29_ss_records/README.md @@ -0,0 +1,27 @@ +# S. schoenii chromosome IV MAT loci: gene models rebuilt from public RNA-seq (2026-10-30) + +**Why:** Kriti et al., G3 2026 ([10.1093/g3journal/jkag067](https://doi.org/10.1093/g3journal/jkag067), PMID 41849659) describe SLA2-MAT cassettes on every chromosome of *S. schoenii* (GDD12, GCA_054953955.1, WGS JBTFFQ01) and say the BRAKER2 GTF is on GitHub and Zenodo. Neither holds it (GitHub `dkriti/Saccharomycopsis-schoenii-Genome-Assembly` and Zenodo 10.5281/zenodo.18950794 carry scripts only; checked 2026-10-29), and the NCBI assembly has no annotation. The models below are rebuilt here and are **ours, not the authors'**. + +**Method** (`ss_rnaseq.slurm`, `ss_models.slurm`, `chr4_orfs.py`, `chr4_models.py`, `make_refs.sh`): the three paired non-stranded RNA-seq runs of PRJNA1364152 (SRR37453601-03; 86% overall alignment) mapped with hisat2; StringTie transcripts; TransDecoder long ORFs; ORFs identified by phmmer against the 63 curated Saccharomycotina MAT proteins (`refs.faa`) and Pfam-A (HMG_box, Homeodomain, MATalpha_HMGbox, I_LWEQ). The library is not stranded, so transcript and ORF strands are inferred from the ORF alone. + +## What agrees with the paper +- Chromosome IV is CM143129.1. Splice junction **536,635-536,692 (58 bp, 218 reads)** = the paper's conserved 58 bp intron at 536,635. The paper's mirrored junction at 529,128 is not seen (the two arms are an inverted repeat, so reads map to one copy). +- RNA depth in the left cluster ends near 474,500 (paper: SLA2 to 475,000); the right cassette has two blocks, 530-532 kb and 534-536 kb, with no depth at 532.5-533.5 kb (the paper's spacer). +- SLA2 (I_LWEQ domain, 546-712 bits to curated SLA2) is found as one ORF in each arm of the palindrome (CDS 529,992-532,490 and 533,981-535,828, mirrored) and, diverged, at 472,708-474,606: the paper's "SLA2 anchors". + +## Models (ours) +| locus | ORF | CDS (CM143129.1) | evidence | +|---|---|---|---| +| left | MATalpha1 | 471,020-471,595, minus | 191 aa, MATalpha_HMGbox 54 bits, 73 bits to *Ogataea* MATalpha1 | +| left | HMG-like (a2-type) | 470,349-470,951 plus, or 470,176-470,243 + 470,336-470,951 with a 92 bp intron (97 reads) | 200/227 aa, 44 bits to *S. cerevisiae* MATA2, no Pfam hit | +| left | homeodomain | 471,961-472,227 + 472,445-472,489 plus | 103 aa (partial), Homeodomain 57 bits; the 217 bp intron (198 reads) joins it toward SLA2, the paper's "MATa1-SLA2 splice junction" | +| left | SLA2 | 472,708-473,478 and 473,680-474,606 | two diverged pieces, I_LWEQ 186 bits | +| right (IR copy 2) | homeodomain | 536,046-536,090 + 536,296-536,634 + 536,693-536,944, minus | 211 aa, Homeodomain 54 bits, introns of 205 bp (221 reads) and 58 bp (218 reads) | +| right (IR copy 1) | homeodomain | 529,258-529,749 | 163 aa, 34 bits; the 58 bp intron is not seen here | +Raw table: `data/chr4_orfs.tsv`; junctions `data/junctions_chrIV.tsv`; transcripts `data/stringtie.gtf`. + +## What is not settled +- **Gene names.** A homeodomain protein is MATa1 or MATalpha2; Pfam cannot say which. The paper calls the left cluster alpha (MATalpha2, MATalpha1) and the right cassette a (MATa1, MATa2). I use those names only by position, as a hypothesis. +- **No HMG-box (MATa2) ORF in the right cassette** passed the filters; the left "HMG-like" ORF has no Pfam hit, as for *Komagataella* MATa2 (13.6 bits, below GA). +- Palindrome copies are collapsed by short-read mapping; strand of the unstranded transcripts is inferred. +- These are our own ORF calls, not an annotation of the deposit, so a record built on them is **tier 3**, not tier 2, until the authors' GTF is obtained or the models are checked another way. diff --git a/results/2026-10-29_ss_records/chr4_models.py b/results/2026-10-29_ss_records/chr4_models.py new file mode 100644 index 00000000..f6b0aabc --- /dev/null +++ b/results/2026-10-29_ss_records/chr4_models.py @@ -0,0 +1,39 @@ +"""S. schoenii chromosome IV (CM143129.1): StringTie transcripts and TransDecoder ORFs in the MAT region, identified by phmmer +against the curated Saccharomycotina MAT proteins (refs.faa). Usage: chr4_models.py data/ refs.faa A B""" +import re, sys, collections, pyhmmer +from pyhmmer.easel import SequenceFile, Alphabet, TextSequence, DigitalSequenceBlock +d, refs, A, B = sys.argv[1], sys.argv[2], int(sys.argv[3]), int(sys.argv[4]) +tx = {} +for l in open(f"{d}/stringtie.gtf"): + f = l.split("\t") + if f[0] != "CM143129.1" or f[2] not in ("transcript", "exon"): continue + tid = re.search(r'transcript_id "([^"]+)"', f[8]).group(1) + t = tx.setdefault(tid, {"strand": f[6], "exons": [], "span": None, "cov": None}) + if f[2] == "transcript": + t["span"] = (int(f[3]), int(f[4])); t["cov"] = float(re.search(r'cov "([^"]+)"', f[8]).group(1)) + else: t["exons"].append((int(f[3]), int(f[4]))) +keep = {k: v for k, v in tx.items() if v["span"] and A <= v["span"][0] and v["span"][1] <= B} +pep = {} +name = None +for l in open(f"{d}/longest_orfs.pep"): + if l.startswith(">"): + name = l[1:].split()[0]; hdr = l; pep[name] = [hdr.strip(), ""] + elif name: pep[name][1] += l.strip() +alpha = Alphabet.amino() +q = [] +for tid in keep: + for n, (h, s) in pep.items(): + if n.startswith(tid + ".p") and s: + q.append(TextSequence(name=n.encode(), sequence=s.rstrip("*")).digitize(alpha)) +with SequenceFile(refs, digital=True, alphabet=alpha) as sf: db = sf.read_block() +out = {} +for hits in pyhmmer.phmmer(q, db, E=1e-2): + qn = hits.query.name if isinstance(hits.query.name, str) else hits.query.name.decode() + if len(hits): + h = hits[0]; out[qn] = (h.name if isinstance(h.name, str) else h.name.decode(), h.score, h.evalue) +for tid, t in sorted(keep.items(), key=lambda kv: kv[1]["span"]): + print(tid, t["strand"], t["span"], f"cov={t['cov']:.0f}", "exons=" + ",".join(f"{a}-{b}" for a, b in sorted(t["exons"]))) + for n, (h, s) in pep.items(): + if n.startswith(tid + ".p") and s: + hit = out.get(n) + print(" ", h[:110].split(" ")[0], h.split()[-2] if False else "", f"len={len(s.rstrip('*'))}", ("-> %s %.0f bits E=%.1g" % (hit[0].split("|")[0][-45:] + ("|" + hit[0].split("name=")[1].split("|")[0] if "name=" in hit[0] else ""), hit[1], hit[2])) if hit else "") diff --git a/results/2026-10-29_ss_records/chr4_orfs.py b/results/2026-10-29_ss_records/chr4_orfs.py new file mode 100644 index 00000000..b3dc275e --- /dev/null +++ b/results/2026-10-29_ss_records/chr4_orfs.py @@ -0,0 +1,55 @@ +"""ORFs of the StringTie transcripts in a window of S. schoenii chromosome IV with genomic CDS coordinates, Pfam domains +(HMG_box, Homeodomain, MATalpha_HMGbox, ANTH/ENTH, I/LWEQ) and the best curated MAT reference hit. +Usage: chr4_orfs.py data refs.faa Pfam-A.hmm A B""" +import re, sys, pyhmmer +from pyhmmer.easel import SequenceFile, Alphabet, TextSequence +d, refs, pfam, A, B = sys.argv[1], sys.argv[2], sys.argv[3], int(sys.argv[4]), int(sys.argv[5]) +tx = {} +for l in open(f"{d}/stringtie.gtf"): + f = l.split("\t") + if f[0] != "CM143129.1" or f[2] != "exon": continue + tid = re.search(r'transcript_id "([^"]+)"', f[8]).group(1) + tx.setdefault(tid, {"strand": f[6], "ex": []})["ex"].append((int(f[3]), int(f[4]))) +tx = {k: v for k, v in tx.items() if A <= min(a for a, b in v["ex"]) and max(b for a, b in v["ex"]) <= B} +def to_genome(tid, s, e): # 1-based transcript coords (transcript orientation = genome + order of exons, '+' transcripts; '-' reversed) + ex = sorted(tx[tid]["ex"]); strand = tx[tid]["strand"] + blocks, off = [], 0 + seq = ex if strand != "-" else ex[::-1] + for a, b in seq: + L = b - a + 1 + lo, hi = max(s, off + 1), min(e, off + L) + if lo <= hi: + blocks.append((a + (lo - off - 1), a + (hi - off - 1)) if strand != "-" else (b - (hi - off - 1), b - (lo - off - 1))) + off += L + return sorted(blocks) +orfs = []; name = None +for l in open(f"{d}/longest_orfs.pep"): + if l.startswith(">"): + m = re.match(r">(\S+) type:(\S+) len:(\d+) \S+ (\S+):(\d+)-(\d+)\(([+-])\)", l) + name = m.group(1); tid = name.rsplit(".p", 1)[0] + orfs.append([name, tid, m, ""]) + else: orfs[-1][3] += l.strip() +orfs = [o for o in orfs if o[1] in tx and len(o[3].rstrip("*")) >= 60] +alpha = Alphabet.amino() +seqs = {o[0]: TextSequence(name=o[0].encode(), sequence=o[3].rstrip("*")).digitize(alpha) for o in orfs} +with SequenceFile(refs, digital=True, alphabet=alpha) as sf: db = sf.read_block() +best = {} +for hits in pyhmmer.phmmer(list(seqs.values()), db, E=1e-2): + if len(hits): + h = hits[0]; best[hits.query.name.decode() if isinstance(hits.query.name, bytes) else hits.query.name] = (h.name.decode() if isinstance(h.name, bytes) else h.name, h.score) +dom = {} +want = {"HMG_box", "Homeodomain", "MATalpha_HMGbox", "ANTH", "ENTH", "I_LWEQ", "HMG_box_2", "Homeobox_KN"} +with pyhmmer.plan7.HMMFile(pfam) as hf: + for hits in pyhmmer.hmmscan(list(seqs.values()), hf, E=1e-3): + q = hits.query.name.decode() if isinstance(hits.query.name, bytes) else hits.query.name + dom[q] = [((h.name.decode() if isinstance(h.name, bytes) else h.name), round(h.score, 1)) for h in hits if (h.name.decode() if isinstance(h.name, bytes) else h.name) in want] +print("orf\ttranscript\tstrand\tlen\tcds_blocks\tbest_ref\tbits\tpfam") +for name, tid, m, s in orfs: + ts, te, st = min(int(m.group(5)), int(m.group(6))), max(int(m.group(5)), int(m.group(6))), m.group(7) + blocks = to_genome(tid, ts, te) if True else [] + br = best.get(name) + ref = "" + if br: + ref = br[0].split("|")[0][-40:] + ("|" + br[0].split("name=")[1].split("|")[0] if "name=" in br[0] else "|" + br[0].split("|")[-1]) + gs = {("+","+"):"+",("+","-"):"-",("-","+"):"-",("-","-"):"+"}.get((tx[tid]["strand"], st), "?") + print(name, tid, gs, len(s.rstrip('*')), ";".join(f"{a}-{b}" for a, b in blocks), ref, f"{br[1]:.0f}" if br else "", dom.get(name, ""), sep="\t") diff --git a/results/2026-10-29_ss_records/data/chr4_models.txt b/results/2026-10-29_ss_records/data/chr4_models.txt new file mode 100644 index 00000000..e6ac03af --- /dev/null +++ b/results/2026-10-29_ss_records/data/chr4_models.txt @@ -0,0 +1,65 @@ +SS.1982.1 + (469005, 473478) cov=85 exons=469005-472227,472445-473478 + >SS.1982.1.p1 len=256 -> 1173061_clib918_MATdc_A|sla2 273 bits E=9e-84 + >SS.1982.1.p2 len=248 + >SS.1982.1.p3 len=200 -> 4932_s288c_MATsc_HMRa|MATA2 44 bits E=8e-14 + >SS.1982.1.p4 len=191 -> 1005962_dl-1_MATog_MATa-MATalpha|MATalpha1 73 bits E=9e-23 + >SS.1982.1.p5 len=103 -> 5476_sc5314_MTL_A_MTLA1 50 bits E=1e-15 + >SS.1982.1.p6 len=98 + >SS.1982.1.p7 len=80 + >SS.1982.1.p8 len=73 + >SS.1982.1.p9 len=73 +SS.1982.2 + (469005, 473478) cov=30 exons=469005-470243,470336-472227,472445-473478 + >SS.1982.2.p1 len=256 -> 1173061_clib918_MATdc_A|sla2 273 bits E=9e-84 + >SS.1982.2.p2 len=248 + >SS.1982.2.p3 len=227 -> 4932_s288c_MATsc_HMRa|MATA2 43 bits E=1e-13 + >SS.1982.2.p4 len=191 -> 1005962_dl-1_MATog_MATa-MATalpha|MATalpha1 73 bits E=9e-23 + >SS.1982.2.p5 len=103 -> 5476_sc5314_MTL_A_MTLA1 50 bits E=1e-15 + >SS.1982.2.p6 len=98 + >SS.1982.2.p7 len=80 + >SS.1982.2.p8 len=73 + >SS.1982.2.p9 len=73 +SS.1983.1 . (473679, 474606) cov=37 exons=473679-474606 + >SS.1983.1.p1 len=309 -> 460519_cbs-7435_MATkp_MATa|sla2 302 bits E=1e-92 + >SS.1983.1.p2 len=197 +SS.1981.1 . (483659, 484889) cov=56 exons=483659-484889 +SS.1984.1 . (485926, 486704) cov=205 exons=485926-486704 +SS.1985.1 . (487454, 487699) cov=11 exons=487454-487699 +SS.1986.1 . (503375, 504795) cov=41 exons=503375-504795 + >SS.1986.1.p1 len=163 -> 5476_sc5314_MTL_A_MTLA1 34 bits E=8e-11 + >SS.1986.1.p2 len=108 -> MH260578_SLA2 109 bits E=3e-34 + >SS.1986.1.p3 len=106 + >SS.1986.1.p4 len=102 + >SS.1986.1.p5 len=80 +SS.1987.1 . (513657, 515192) cov=43 exons=513657-515192 + >SS.1987.1.p1 len=201 + >SS.1987.1.p2 len=165 + >SS.1987.1.p3 len=134 + >SS.1987.1.p4 len=130 + >SS.1987.1.p5 len=82 + >SS.1987.1.p6 len=70 + >SS.1987.1.p7 len=69 +SS.2000.1 . (522696, 524063) cov=17 exons=522696-524063 + >SS.2000.1.p1 len=112 + >SS.2000.1.p2 len=61 +SS.2001.1 . (528841, 532491) cov=143 exons=528841-532491 + >SS.2001.1.p1 len=832 -> 460519_cbs-7435_MATkp_MATa|sla2 712 bits E=5e-216 + >SS.2001.1.p2 len=219 + >SS.2001.1.p3 len=191 + >SS.2001.1.p4 len=163 -> 5476_sc5314_MTL_A_MTLA1 34 bits E=1e-10 + >SS.2001.1.p5 len=116 + >SS.2001.1.p6 len=106 + >SS.2001.1.p7 len=80 + >SS.2001.1.p8 len=71 +SS.2002.1 - (533930, 536977) cov=32 exons=533930-536634,536693-536977 + >SS.2002.1.p1 len=632 -> 1173061_clib918_MATdc_A|sla2 571 bits E=8e-174 + >SS.2002.1.p2 len=271 -> 5476_sc5314_MTL_A_MTLA1 33 bits E=2e-10 + >SS.2002.1.p3 len=197 + >SS.2002.1.p4 len=191 + >SS.2002.1.p5 len=106 + >SS.2002.1.p6 len=80 +SS.2002.2 - (533980, 536977) cov=160 exons=533980-536090,536296-536634,536693-536977 + >SS.2002.2.p1 len=615 -> 1173061_clib918_MATdc_A|sla2 546 bits E=3e-166 + >SS.2002.2.p2 len=211 -> 5476_sc5314_MTL_A_MTLA1 47 bits E=9e-15 + >SS.2002.2.p3 len=191 + >SS.2002.2.p4 len=181 + >SS.2002.2.p5 len=80 diff --git a/results/2026-10-29_ss_records/data/chr4_orfs.tsv b/results/2026-10-29_ss_records/data/chr4_orfs.tsv new file mode 100644 index 00000000..9f503158 --- /dev/null +++ b/results/2026-10-29_ss_records/data/chr4_orfs.tsv @@ -0,0 +1,54 @@ +orf transcript strand len cds_blocks best_ref bits pfam +SS.1982.1.p1 SS.1982.1 - 256 472708-473478 1173061_clib918_MATdc_A|sla2 273 [('I_LWEQ', 185.9)] +SS.1982.1.p2 SS.1982.1 + 248 469005-469751 [] +SS.1982.1.p3 SS.1982.1 + 200 470349-470951 4932_s288c_MATsc_HMRa|MATA2 44 [] +SS.1982.1.p4 SS.1982.1 - 191 471020-471595 1005962_dl-1_MATog_MATa-MATalpha|MATalpha1 73 [('MATalpha_HMGbox', 54.2)] +SS.1982.1.p5 SS.1982.1 + 103 471961-472227;472445-472489 5476_sc5314_MTL_A_MTLA1|5476_sc5314_MTL_A_MTLA1 50 [('Homeodomain', 56.8), ('Homeobox_KN', 34.3)] +SS.1982.1.p6 SS.1982.1 - 98 469070-469366 [] +SS.1982.1.p7 SS.1982.1 - 80 471942-472184 [] +SS.1982.1.p8 SS.1982.1 + 73 469958-470179 [] +SS.1982.1.p9 SS.1982.1 + 73 473258-473476 [] +SS.1982.2.p1 SS.1982.2 - 256 472708-473478 1173061_clib918_MATdc_A|sla2 273 [('I_LWEQ', 185.9)] +SS.1982.2.p2 SS.1982.2 + 248 469005-469751 [] +SS.1982.2.p3 SS.1982.2 + 227 470176-470243;470336-470951 4932_s288c_MATsc_HMRa|MATA2 43 [] +SS.1982.2.p4 SS.1982.2 - 191 471020-471595 1005962_dl-1_MATog_MATa-MATalpha|MATalpha1 73 [('MATalpha_HMGbox', 54.2)] +SS.1982.2.p5 SS.1982.2 + 103 471961-472227;472445-472489 5476_sc5314_MTL_A_MTLA1|5476_sc5314_MTL_A_MTLA1 50 [('Homeodomain', 56.8), ('Homeobox_KN', 34.3)] +SS.1982.2.p6 SS.1982.2 - 98 469070-469366 [] +SS.1982.2.p7 SS.1982.2 - 80 471942-472184 [] +SS.1982.2.p8 SS.1982.2 + 73 469958-470179 [] +SS.1982.2.p9 SS.1982.2 + 73 473258-473476 [] +SS.1983.1.p1 SS.1983.1 ? 309 473680-474606 460519_cbs-7435_MATkp_MATa|sla2 302 [] +SS.1983.1.p2 SS.1983.1 ? 197 474014-474604 [] +SS.1986.1.p1 SS.1986.1 ? 163 503715-504206 5476_sc5314_MTL_A_MTLA1|5476_sc5314_MTL_A_MTLA1 34 [('Homeodomain', 32.0)] +SS.1986.1.p2 SS.1986.1 ? 108 504467-504793 MH260578_SLA2|MH260578_SLA2 109 [('I_LWEQ', 94.3)] +SS.1986.1.p3 SS.1986.1 ? 106 503948-504268 [] +SS.1986.1.p4 SS.1986.1 ? 102 503375-503683 [] +SS.1986.1.p5 SS.1986.1 ? 80 503696-503938 [] +SS.1987.1.p1 SS.1987.1 ? 201 514179-514784 [] +SS.1987.1.p2 SS.1987.1 ? 165 513658-514152 [] +SS.1987.1.p3 SS.1987.1 ? 134 514786-515190 [] +SS.1987.1.p4 SS.1987.1 ? 130 514123-514515 [] +SS.1987.1.p5 SS.1987.1 ? 82 513659-513907 [] +SS.1987.1.p6 SS.1987.1 ? 70 514847-515059 [] +SS.1987.1.p7 SS.1987.1 ? 69 513915-514124 [] +SS.2000.1.p1 SS.2000.1 ? 112 523396-523734 [] +SS.2000.1.p2 SS.2000.1 ? 61 523205-523390 [] +SS.2001.1.p1 SS.2001.1 ? 832 529992-532490 460519_cbs-7435_MATkp_MATa|sla2 712 [('I_LWEQ', 182.4)] +SS.2001.1.p2 SS.2001.1 ? 219 531298-531957 [] +SS.2001.1.p3 SS.2001.1 ? 191 530542-531117 [] +SS.2001.1.p4 SS.2001.1 ? 163 529258-529749 5476_sc5314_MTL_A_MTLA1|5476_sc5314_MTL_A_MTLA1 34 [('Homeodomain', 33.9)] +SS.2001.1.p5 SS.2001.1 ? 116 528876-529226 [] +SS.2001.1.p6 SS.2001.1 ? 106 529491-529811 [] +SS.2001.1.p7 SS.2001.1 ? 80 529239-529481 [] +SS.2001.1.p8 SS.2001.1 ? 71 531843-532058 [] +SS.2002.1.p1 SS.2002.1 + 632 533930-535828 1173061_clib918_MATdc_A|sla2 571 [('I_LWEQ', 183.0)] +SS.2002.1.p2 SS.2002.1 - 271 536071-536634;536693-536944 5476_sc5314_MTL_A_MTLA1|5476_sc5314_MTL_A_MTLA1 33 [('Homeodomain', 33.1)] +SS.2002.1.p3 SS.2002.1 - 197 533932-534522 [] +SS.2002.1.p4 SS.2002.1 - 191 534703-535278 [] +SS.2002.1.p5 SS.2002.1 - 106 536009-536329 [] +SS.2002.1.p6 SS.2002.1 + 80 536339-536581 [] +SS.2002.2.p1 SS.2002.2 + 615 533981-535828 1173061_clib918_MATdc_A|sla2 546 [('I_LWEQ', 183.1)] +SS.2002.2.p2 SS.2002.2 - 211 536046-536090;536296-536634;536693-536944 5476_sc5314_MTL_A_MTLA1|5476_sc5314_MTL_A_MTLA1 47 [('Homeodomain', 54.2), ('Homeobox_KN', 33.1)] +SS.2002.2.p3 SS.2002.2 - 191 534703-535278 [] +SS.2002.2.p4 SS.2002.2 - 181 533980-534522 [] +SS.2002.2.p5 SS.2002.2 + 80 536339-536581 [] diff --git a/results/2026-10-29_ss_records/data/depth_chrIV_455k-545k.tsv.gz b/results/2026-10-29_ss_records/data/depth_chrIV_455k-545k.tsv.gz 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+MLSFGSHSNLDIQASLKKACTADAAAPKRKHVRACIVYTWDHKSSREFWHCLKLLPIQSNDTQIFKTLIVIHKVLQEGHPTCLIGGYKNISWLESLSRFSNNGTAAGYTRLIKEYVFYLEQKLKFHHDHRGFNGMFEYEEYVSLRTVSDPNEGFESIMDLLSLQDSLDNLQRVIFSSIRHTSESECVISSLVPIIAESYGIYKFLISMLKALYKSSESDEVIAPLKDRFDVQHRRLFEFYADCSSIKYLTTLVTIPRLPSSPPILQDEEDPQGFPLVRERSSVSNSEESNIPRSSNIQSQPTGAMVDSFVRQQQELEEQQRQAEERRQAELLEQQMLQRDQARFWEEQQRQQAVSQELAQSQLLADQMQRQAQGKLAQLEQDLLTLGGQHERDQLMLEQYDQRVKVLENELRIVTQTAEEQLAVKTSQCSHLEEQVQYWKNKYESLAKLYSQLRQEHLNLLGKFKIVQQKAASAQESIDKREKLEKDLKAKNIELADLIKERDRARLDLARIKGNDSEQVQMLRTQLRDMETKLKSLEETHSQNLASLFSQHSKEIEEIKASASELKEGGLNEEAQQEVQMLQQAMDDTMQELAEQHQAIEKEAETRVLRLIDTLLKVSSDRLREFVFNLESPVYSNTDIPTAEYLGTMLERVSHLSTQFSTTMTDYIADNMKGESESGLIKAIIELTDSIGNLFSNVKAVLRDRKDDVQEILVEGSKDLAHMVELFFESMLSNHLLKLEIHERTDRVITGNINVQEVIQSLMQVVEVFRDPGRKTPVSSDELGYFLDKELANTQAAVEKAAGLLESMSTRHKLEVQEDGSLEVNEAIISCASAIINAVKLLLEACIDAQEEIVSRGRGSMSRALFYKKNNRWTEGLISASKQVAYATGILIRMADGVLAGTNSSEELIVASNEVASATAQLVSSSRVKSDLMSQSHLNLEEASRKVTSSCKMLVTKVRSLLLSDESNKTVDYSQLTAHENRTAELEQQVEILKLESALSHARKRLGEIRKFSYRDEES +>1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT +MQFIILNEPSLDSQRREGNLASENCVFARDIRKEGVKVLEDSLRSERNALLAIGAGGAVDRVERHYRRLLDGWEAAIMEIRSNDKYESLWRGFAIEKAEVETEMRKVEKQWPGELDVYKECFVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT +MFLAFFVIARVGLQNLCLQRKFPPHGDSAKMALDGLNLSFLTHLPQIPTTSPLLKRLLSEYGAIDNGNDSSNKVAPKKSRRHRVQKQKKRLNGFMAFRSFYSRNIANYNSQKALSQDLADAWNREKHQQIWSLYAIQYNHSKSKEPFSIWLEMKLNKKLRKNNSDDPTTRREINIMSDIVVEDVFQECSS +>1005962_dl-1_MATog_MATa-MATalpha|gene_index=3|name=MATalpha2|role=core_MAT +MSSYEEQCSAPDLLLLLDRLIDSITESDLSLEDELYWDSHVHSLMAVITHTVSSPSGTPMDDMLGQIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKFCKSSMESFTTERTIEYTRDCPPYAAVTTSQPGYKTEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTDCSPNDVV +>1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved +MNSDKSTKSIAPSANFPFWYGGAASMFACLFTHPLDLAKVRLQTAKVPGDSLVSLAFKIIKTEGVLAAYAGLTASLLRQATYSTARFGVYEKLKEIMTDPTRGQASTFQLLAASMIAGAVGGVVGNPADVVNIRMQNDNSLPESQRRHYKHALDGLLKITREENLTALFRGLGPNLARGILMTASQVVSYDVAKKLLVENLSMDPKTKATHFSASLIAGLVATTVCSPADVLKTRIMNSSGTGQSSFGILKDAISREGLGFMFRGWTPAFIRLGPHTILTFIALEELRRLKIGL +>460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved +MTQINRESNDDSTMQYERIPPAKGVFPFWYGGAASMFACLFTHPLDLAKVRLQTASQPGQNLLTIAIRIISSEGILGAYSGLSASLLRQATYSTTRFGIYEALKDYLTKANNNQDLSTAILLPCSMLAGGIGGLVGNPSDVVNIRMQNDSSLPKPARRNYRNALHGLARMAKEEGLGSWFRGLLPNLTRGVLMTASQVVSYDVAKKFLVKKLSFDETTRATHFSASLMAGLVATTVCSPADVVKTRIMNSAEKHQSIITVLTTAMRKEGPGFLFRGWFPSFIRLGPHTILTFVALEQLRTWRIAM +>460519_cbs-7435_MATkp_MATa|gene_index=1|name=MATa2|role=core_MAT +MTVTTFLSSSFDSVAFAMTCVSDKSYKTTAGKGLSGKKFNLSTHRPRNKFIISKSIFSKVIQRYYPDLSFEENSKIASYLWKHRFGCFDKYYEYLQIEESKFTSLVTSKIDQLAFKAVPTEKTTSQCTFRFSSSPKRRRTVNLNILKPLCKRVYSQQFHSPLTGITTQDIFVAEENNV +>460519_cbs-7435_MATkp_MATa|gene_index=2|name=MATa1|role=core_MAT +MSGNTFREITAACLLSMILSTDDYTKPATSCHMHQLTAPDLSDYSLLDALFVTCDRALEYQALTYRLQASMEDAMKIIESARYQTKDLPTLDNIFKTFYKTLLDTIESIEATGTSTLLSDFTHQISFFNIDPSVISLSDETKECAYSFFSDSLHHHPVKRTRKTISKEVKKILEEAFKAKAAPNTKERLLIAQRCALTPLQVRMWFTNKRTRNKIKQRLQAQSHP +>460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved +MSRQEADLKVSIKKACSTEEAAPKRKHVRACIVFTWDHRSSKAFYNGLRLLPIQNDEIPLFKSLITIHKVLQEGHPSAIKEGIKNRDWIQSLGHVFPGDGMKRYGRLIREYDRYLIRKIDFHNSHKGFNGTFEYEEYVSLKTVSDPNEGYEAIMDLMVLQDSINDLQRLLFASIDSSSHSELKISALVPLIAESYGIFKFITSMLRGMCHSLGHDDEALQPLIERFISQYSDLYDFYAECSYVKYLTSLVNIPKLPFEAPDLFNWDETDELKPRILAEEPSLQVSSKAPSEEFGTPTEVIHSQPTAGTDLWAQSNALKQQYEQEQQLLEQERQQQLLEQQHQQQQQLEYWEKQKELQEQQQLYAQQQLESDQLQRQVQGRVGELERDLLILRGQFDRDQLMLRQYDERVKALETELSSTTQNAQQQLGAKEELLGNIQEQAAQWKSKYDSLAKLYSQLREEHLNLLSKFKTAQLKASSAREAIDKREKIERDLKAKNIELADLIRERDRARLDLERSKSDKNSETERLEARLREQETKLADIEKLHSSELKSFLSQHNKEIEELQNQLQASSLSESANERLKALEAQLQEKDDELAIAHQTMEATIKELTGQQSIQNSAIDEQIDAILLEHIDKLTEVIDAILTSGVQRIQDGLFELDSPMQAGNQNSSPEYLSSIIEKSSNLATEFTSSFNGFIADGPNGDHVSVIQTITEFTTSIVDLLLNCKGVVRLASSDQEAEALLNSARDVAEESESFIVSLYSSSLKGLSDQEKTDKAIEGNVDIQQELQTLSSLVEKLISSQVNINVNDNVGELVDREMAHAAETIAKASDHLTDLLTNAKQDISTIDMKINNSILSAAIAVTKAIILLIKTATDCQNEIVNHGRGSSSRTSFYKKHNKWTEGLISASKSVAYSTNSLIQIADGVLSSTSSPEQLIVACNDVAASTAQLVAASRVKASFMSKTQDKLELASKSVTSACRSLVRQVQEILSQKDKEELEIDYSKLSAHENKTVQMEQQVEILKLETALQNARRRLGEIRRFGYHADEE +>460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved +MSRQEADLKVSIKKACSTEEAAPKRKHVRACIVFTWDHRSSKAFYNGLRLLPIQNDEIPLFKSLITIHKVLQEGHPSAIKEGIKNRDWIQSLGHVFPGDGMKRYGRLIREYDRYLIRKIDFHNSHKGFNGTFEYEEYVSLKTVSDPNEGYEAIMDLMVLQDSINDLQRLLFASIDSSSHSELKISALVPLIAESYGIFKFITSMLRGMCHSLGHDDEALQPLIERFISQYSDLYDFYAECSYVKYLTSLVNIPKLPFEAPDLFNWDETDELKPRILAEEPSLQVSSKAPSEEFGTPTEVIHSQPTAGTDLWAQSNALKQQYEQEQQLLEQERQQQLLEQQHQQQQQLEYWEKQKELQEQQQLYAQQQLESDQLQRQVQGRVGELERDLLILRGQFDRDQLMLRQYDERVKALETELSSTTQNAQQQLGAKEELLGNIQEQAAQWKSKYDSLAKLYSQLREEHLNLLSKFKTAQLKASSAREAIDKREKIERDLKAKNIELADLIRERDRARLDLERSKSDKNSETERLEARLREQETKLADIEKLHSSELKSFLSQHNKEIEELQNQLQASSLSESANERLKALEAQLQEKDDELAIAHQTMEATIKELTGQQSIQNSAIDEQIDAILLEHIDKLTEVIDAILTSGVQRIQDGLFELDSPMQAGNQNSSPEYLSSIIEKSSNLATEFTSSFNGFIADGPNGDHVSVIQTITEFTTSIVDLLLNCKGVVRLASSDQEAEALLNSARDVAEESESFIVSLYSSSLKGLSDQEKTDKAIEGNVDIQQELQTLSSLVEKLISSQVNINVNDNVGELVDREMAHAAETIAKASDHLTDLLTNAKQDISTIDMKINNSILSAAIAVTKAIILLIKTATDCQNEIVNHGRGSSSRTSFYKKHNKWTEGLISASKSVAYSTNSLIQIADGVLSSTSSPEQLIVACNDVAASTAQLVAASRVKASFMSKTQDKLELASKSVTSACRSLVRQVQEILSQKDKEELEIDYSKLSAHENKTVQMEQQVEILKLETALQNARRRLGEIRRFGYHADE +>460519_cbs-7435_MATkp_MATalpha|gene_index=1|name=MATalpha1|role=core_MAT +MLPPQPKSFKGIQKPFKCSQYAHSTLDNGNMLQHTKHPELVTYQLETPRTRSLQQIESHLLQETTLGIKRTGENQYSEKRTKSSRSYMSKRINAFIAFRSYYSRQVNDYNKQISISRLLSVAWKNENKELWRSYALLYNSSNSNIPFVDWLQTKGPKNSKRTSTVTKSFISWNELLSTRNKIEDVFLDLQ +>460519_cbs-7435_MATkp_MATalpha|gene_index=2|name=MATalpha2|role=core_MAT +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRAIALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLSKTQIRNWISNKRRKERSSTVSTELLQALNKSPS +>460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved +MTQINRESNDDSTMQYERIPPAKGVFPFWYGGAASMFACLFTHPLDLAKVRLQTASQPGQNLLTIAIRIISSEGILGAYSGLSASLLRQATYSTTRFGIYEALKDYLTKANNNQDLSTAILLPCSMLAGGIGGLVGNPSDVVNIRMQNDSSLPKPARRNYRNALHGLARMAKEEGLGSWFRGLLPNLTRGVLMTASQVVSYDVAKKFLVKKLSFDETTRATHFSASLMAGLVATTVCSPADVVKTRIMNSAEKHQSIITVLTTAMRKEGPGFLFRGWFPSFIRLGPHTILTFVALEQLRTWRIAM +>460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved +MTQINRESNDDSTMQYERIPPAKGVFPFWYGGAASMFACLFTHPLDLAKVRLQTASQPGQNLLTIAIRIISSEGILGAYSGLSASLLRQATYSTTRFGIYEALKDYLTKANNNQDLSTAILLPCSMLAGGIGGLVGNPSDVVNIRMQNDSSLPKPARRNYRNALHGLARMAKEEGLGSWFRGLLPNLTRGVLMTASQVVSYDVAKKFLVKKLSFDETTRATHFSASLMAGLVATTVCSPADVVKTRIMNSAEKHQSIITVLTTAMRKEGPGFLFRGWFPSFIRLGPHTILTFVALEQLRTWRIAM +>460519_gs115_MATkp_MATalpha|gene_index=1|name=MATalpha2|role=core_MAT +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRAIALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLSKTQIRNWISNKRRKERSSTVSTELLQALNKSPS +>460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved +MSRQEADLKVSIKKACSTEEAAPKRKHVRACIVFTWDHRSSKAFYNGLRLLPIQNDEIPLFKSLITIHKVLQEGHPSAIKEGIKNRDWIQSLGHVFPGDGMKRYGRLIREYDRYLIRKIDFHNSHKGFNGTFEYEEYVSLKTVSDPNEGYEAIMDLMVLQDSINDLQRLLFASIDSSSHSELKISALVPLIAESYGIFKFITSMLRGMCHSLGHDDEALQPLIERFISQYSDLYDFYAECSYVKYLTSLVNIPKLPFEAPDLFNWDETDELKPRILAEEPSLQVSSKAPSEEFGTPTEVIHSQPTAGTDLWAQSNALKQQYEQEQQLLEQERQQQLLEQQHQQQQQLEYWEKQKELQEQQQLYAQQQLESDQLQRQVQGRVGELERDLLILRGQFDRDQLMLRQYDERVKALETELSSTTQNAQQQLGAKEELLGNIQEQAAQWKSKYDSLAKLYSQLREEHLNLLSKFKTAQLKASSAREAIDKREKIERDLKAKNIELADLIRERDRARLDLERSKSDKNSETERLEARLREQETKLADIEKLHSSELKSFLSQHNKEIEELQNQLQASSLSESANERLKALEAQLQEKDDELAIAHQTMEATIKELTGQQSIQNSAIDEQIDAILLEHIDKLTEVIDAILTSGVQRIQDGLFELDSPMQAGNQNSSPEYLSSIIEKSSNLATEFTSSFNGFIADGPNGDHVSVIQTITEFTTSIVDLLLNCKGVVRLASSDQEAEALLNSARDVAEESESFIVSLYSSSLKGLSDQEKTDKAIEGNVDIQQELQTLSSLVEKLISSQVNINVNDNVGELVDREMAHAAETIAKASDHLTDLLTNAKQDISTIDMKINNSILSAAIAVTKAIILLIKTATDCQNEIVNHGRGSSSRTSFYKKHNKWTEGLISASKSVAYSTNSLIQIADGVLSSTSSPEQLIVACNDVAASTAQLVAASRVKASFMSKTQDKLELASKSVTSACRSLVRQVQEILSQKDKEELEIDYSKLSAHENKTVQMEQQVEILKLETALQNARRRLGEIRRFGYHADE +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved +MEVCITTQSIDNGVGAASMFACLFTHPLDLAKVRLQTAKVPGDSLVSLAYKIVKTEGVLAAYAGLSASLLRQATYSTARFGVYEKLKGIMTDPTKGQASTFQLLAASMIAGAVGGVVGNPADVVNIRMQNDNSLPESQRRHYKHALDGLLKITREENITALFRGLGPNLARGILMTASQVVSYDVAKKLLVENLSMDPKTKATHFSASLIAGLVATTVCSPADVLKTRIMNSSGTGQSSFGILKDAISREGLGFMFRGWTPAFIRLGPHTILTFIALEELRRLKIGL +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=1|name=MATalpha2|role=core_MAT +MDDMLTKIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKLCKSSMEGFTTERSLKYTRDCPQYAAVTTSQPRYRIEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTRCSPNDVV +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT +MMFHVLCFFGNRTSRVANLCLQLKFPPHNDSPKMALDELNLSFLTHLPQIPTTSPLLKRLLTEYGAIDNGKDSLNKVAPKKSSRHRVQKEKKRLNGFMAFRSFYSRNIANYNSQKALSQDLADAWNREKHQQIWSLYAIQYNHSKSKEPFSRWLEMKLSNKLGKFNSDDPTTRREINVMSHIVVEDVFQECSSQY +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT +MQFTILNEPSLDSQRREGDLASENYVFGDIRKEGVRILEDSLRSERNALLAIGAGGAADRVKQHYRKLLDDWEAAIMEIRSNDKYESLCCGFAIEKAEVETEMRNTEELDVYKECLVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved +QMLQQAMDDTMQELAEQHQAIEKEAETRVLRLIDTLLKVSTDRLREFVFNLESPVYSNTDIPTAEYLGTMLEKVSHLSTQFSTTMTDYIADNMRGDSESGLIKAITEFTDSIGNLFSNVKAVLRDRKDDVQEILVEGAKDLAHMVELFFESMLSDHLLKLEIHERTDRVITGNINVQEVIQSLMQVVEVFRDPGRKSPVSSDELGYFLDKELANTQAAVEKAADLLESMSTRHKLEVQEGGSLEVNEAIISCASAIINAVKLLLEACIEAQEEIVSRGKGSLSRALFYKKNNRWTEGLISASKQVAYATGILIRMADGVLAGTNSSEELIVASNEVASATAQLVSSSRVKSDLMSQSHLNLEEASRKVTSSCKMLVTKVRSLLLTDESNKTVDYSQLTAHENRTAELEQQVEILKLESALSQARKRLGEIRKFSYRDEES +>28985_nrrl-y-1140_MATsc_HMLalpha|gene_index=0|name=MATALPHA1|role=core_MAT +MKSNAPTFKVAVSKRSCSSVRKTSKKIRSGMIRKPSVSSRYRKHEGVNLYMSKVTPTSIPAPPQVLVAYIKEKVKTLNKSEVLMSLGNSNQLSSRDVKTKTKKKQINDFIAFRSYYSRLLNGILTQTELSTIISKHWTVDKQTRKNWELIAQEYNCDASGKHFFNWLEVNYGIDKQWLYEIVQYEECLTPTTKKPYVENIYNSGFKQRIDSPNQKDITFSQSDNSADWLVDPIFFDTEFLATSFDRTNLIISDCYSLMNSV +>28985_nrrl-y-1140_MATsc_HMLalpha|gene_index=1|name=MATALPHA2|role=core_MAT +MSRIPIHSLLNPSESCKSISNVPSNYRDLSTFNKERAKVITTFQEMFYSMLENNDDYNKIESLIRNFQPKLTWSHKCESLTFKQKAYLTAIIQKSIKSLLVLLKEKGKMREIEFSRKEVRKINKYRQSSKNFESVNIKILTQDLMHSNNNEFKKGKRFPKSHIQLLENWYSMNRRNPYLAENDLAYISKNTTLTKTQIKNWLANRRRKDKITEVSSDIRNILN +>28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved +MSREEQTLEKSIKKALSGGDVAPKRKHVRACIVYTWDHKSSKTFFNLLKLQPYATDELQLFKALVLLHKVLQEGHPSALVEGIRNIDWIESLSRIFSVDSDDGSKYGSSRMRYIGDEYAKAIGEYVFFLVEKLQFHKSHKGFNGIFEYEEYVSLVSVSDPNEGYETVLDLMNLEDVADNLQKLIFAGISNRKSGFGSFSSGLDCRGAVLIPLVSETYGIFKFVTSMMKALYKQMGDYDALSGLFDRYTEQCARLNEFYFDCNTVQPLKNLITIPTLPSDPMSRIIDDEDDAEVNDVVFEKRSKSSVSSLPSRTPTRENLRDPSPVHEAPPVARPAEVRSLSQPAAPVVPIATGVVMPVVTGYSQPPLQVVPTGAEFWSIPSTSAPTRSVVASHDDLLIEQQKQQQIQQQQYALQLEHQKQLQLQQEQQALLQQRIQQQEMEKEALIQQKTASLQNDIMSFKNQYERDQVMLEQYDKRVQKLESELESVKEFKEKYQSLAKLYSQLRQEHLNILKKMKKTQDDLAEAESEVKKLKSSALSNVGIRNNNKGESEREEAKDRSGMILKSLVESSIKSLQDPIASLDVSDLATDLSNAFIDGDINTIVNILPEFVSLLLRCPQEMQTIAEDFLRCLSSAKQTDSVINANIAFQEKLKRLGEIRQYSYRYGDSDEV +>28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT +MCDNDMADIQSKLSSFCEEIRALALKEGYNLEGDKSPSSKPYFMSWPKEIDVNHPNFAFFTKLQFQYDKSLETILNSCYLQELQLDPTRIIETLQQHFNNSILRYADIENISDVKDESPMSFDTEHECTDTSEDISDKSEISSTNSDNPIQNCYPTYKRSFIKHESRGILEKIFKVKQCPNTSERLYIAQKLDLTPSQVRIWFTNKRMRAKKHTTGKGTKRKSKKYPS +>28985_nrrl-y-1140_MATsc_MATa|gene_index=2|name=MATA2|role=core_MAT +MANSTLRRTTFFKLTTTEDEDTIPKLLQPNNNSVAFPNLKRTGKAFTNDTIKENTKKYTRPRNQFVLMRTLFNRRVNNHILQYYNKSKLEKKMFTLTSKITSELWNESSPDLKSYFSLLATLEENWHKYTHYCSWDRNSAQTLSMEPIELSQVRPRLISSLTVGAGSSVSTYTLRELLLVRKIKSRKRKTTTLTQDSSPTTKFKYKFKKQPKTKMNSNLSKLRFKSKQPPTPPEENSNVFKKRYTSENRIIEDLFLM +>381046_cbs-6340_MATsc_HMLalpha|gene_index=0|name=MATALPHA1|role=core_MAT +MNSTKPSFKVLIKKKGKTLNRRLKNSSKSYRENGANLYMSYSKPQAIPKPPNTLINLVRSKKTSQSSRKCLKNDYILKELDVKKLSIYSNNTVLKKKINPFIGFRSYYAKFAKGRVRQQELSKILSEYWTKNSKIHSVWEFFTQHYNREVTSMCFTLWLEENYQQSYEESELDSECMAKRSQPYIEDLYSGTGEFLTKLTSRELLDISGNHSGFQGSLNSLPFFEEHNRTTDWMLSSILQCDQVPYYPN +>381046_cbs-6340_MATsc_HMLalpha|gene_index=1|name=MATALPHA2|role=core_MAT +MSHISVSSLLNLPVFQASHQEKATSPITSQSCLVASGKQRKFNATAKKRLERWFCSNIQYPYPNKIQILSLSKNTNLSIKQIQNWMSNRRRKSRKESIDSLLEPILNS +>4932_s288c_MATsc_HMRa|gene_index=0|name=MATA2|role=core_MAT +MRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT +MDDICSMAENINRTLFNILGTEIDEINLNTNNLYNFIMESNLTKVEQHTLHKNISNNRLEIYHHIKKEKSPKGKSSISPQARAFLEQVFRRKQSLNSKEKEEVAKKCGITPLQVRVWFINKRMRSK +>4932_s288c_MATsc_MATalpha|gene_index=0|name=MATALPHA2|role=core_MAT +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>4932_s288c_MATsc_MATalpha|gene_index=1|name=MATALPHA1|role=core_MAT +MFTSKPAFKIKNKASKSYRNTAVSKKLKEKRLAEHVRPSCFNIIRPLKKDIQIPVPSSRFLNKIQIHRIASGSQNTQFRQFNKTSIKSSKKYLNSFMAFRAYYSQFGSGVKQNVLSSLLAEEWHADKMQHGIWDYFAQQYNFINPGFGFVEWLTNNYAEVRGDGYWEDVFVHLAL +>4956_da2_MATsc_MATalpha|gene_index=0|name=cha1|role=flanking_variable +RNATPSLEHENIIYPKRAQVAS +>4956_da2_MATsc_MATalpha|gene_index=1|name=alpha2|role=core_MAT +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKEDQKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRSNKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVSELVKESKQGTEELNE +>4956_da2_MATsc_MATalpha|gene_index=2|name=alpha1|role=core_MAT +MNTKSFRGFKSNQPLFKVKVEKKGRSKRPTLQDTTQTSKCIAGKCIEDQQVNVFMTVSKLIKIPSPPEVLLRKIEEEKHKLKKDDFNMDNILLWDPYVYWGEGYFFSIADSSLNNLSTKGSRKGCSEKPLNSFMAFRAYNSQFGNGLKQNILSSLLAAAWHSHPEQQKIWDTFAQQFNFVKPKCGFVEWVDQRYERESCI +>4956_da2_MATsc_MATalpha|gene_index=3|name=sla2|role=flanking_conserved +SSNSNSGMSYGNVVNEEPRPVISGDVRLAIYLSRQCISNYNQPVSVLSISQLCNEVASQFLNSTPHIPTIIQLTHQITTLLQHNPSPLLAQASQLYFQALLSPSQQDIINANTNLQQLLTN +>4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT +MTSEVAKVIPKRFTSRFTVSKTGKRSNSNIKNEAIDIGRQRKVKSMQVVFFTELPSLPTPSIDLQEILIRFNQATRNCAGDSSWGSQLFDMKVPTRKSRIRCKKTRCLNGFMAFRSFYSRSIYNVEHQRELSTLLGKLWKNEPNKAIWNRYAIEYNSQAINQDFVDWLFKALGLEVETFPIPYTSTVRNESWTFSSRNNENAVEDIYYIGTV +>4959_cbs767_MTL_A|gene_index=0|name=MTLA1|role=core_MAT +MEGYTKSHDVVVEVPENENIKAYTTRVESGHISEPSSSNGSNLTLNVKNEYENISSDGKVIDRYQLLKEKGYSHGFLLEEASISVTCETPDPHLQQQRKVSRFGREVHENTNVFPNKKGSYKCDSEASNRFLENVFKTNSYPTSKERHIIAKKCGLSPTQVGAWFTNRRNVSE +>4959_cbs767_MTL_A|gene_index=1|name=MTLA2|role=core_MAT +MSLSPDKIFTFCFKLPRRIDIPVESNQPTTKKNFKIKELTTTNSYPSISINIKQYVSFTSEKYIEASRKPTAKDKKTAITRGSFRTRNKFIIARNLLCRLVKHEKALSSTEISKVVSLVWKESNKSLHDYFEYLSLLETYWYDKLMSSYGNSTLQATATAAVSIRLSSVPYENLCASCYNKHKQKKTLNYDYSKSAKFRVHKDFKEKKKTFLTIKDHSKKLEKLSDAFRINQCTITTNFGIVTEDMFFNSL +>1173061_cbs615-84_MATdc_B|gene_index=0|name=MATB|role=core_MAT +MPEFKRFRFRHHKISCPSTTKNESKFLFCPQFIDFSAPKNGSEECRHTETMLAIPPPSSELLKALDELRTLQCSVQKKGIIRSRRRKPNTNMAKKVSRINGFTAFKSYYSQNITSVCQGTISSKLSKVWQIEKNQDVWDNYARHYRRYQRKEPEHFCVWLLRATQNNSIDKQKTHSFTKLNDGDENLDIEALCFNLNEDSFTKDLITTQSSGDLNPYSNYGFDTLDFITDSSKVSSDNIESSNMNDYMDGLSPESCESISPFDIIDSTFSEPTSNHYDYMFIDSPSDSLLEDFFSDKNVVSDKNCKQPFVPLELNKDQFLPMPELWVSLNSEYSLSKLS +>1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved +MATLSKAEAELTVHIKKATNPEETAPKRKHVRQCIIYTWDNHSGRAFWNGMKIQPLQNDEVQVFKALITIHKVLQEGHPSVLREAQQNRSWLESLARGAHAGRHTYAKLIYEYVGLLLKKLNFHKNHPDFNGTFEYEEYISLRNIDDPNEGYQAIVDLMDLQDAIDNFQRLIIASLKHGRPNECRISALIPLVAESYGIYRFTTTMLRAMHATTNDDDALEPLRSRYYSQHQRLLEFYYDCSSLKYLTSVITIPTLSQDPPNLYGENPEEGEAPALPKRPISEVASLATGATTAPSTTDLTPEPVVDFWSTPQGQQQLQQQQLQQQQYEEEQRRLAAQREAELQQQQMLHLQQQQQFEEQQRMQAEQQRLAQEALLRDQYQRQADGRAAELERDILNLRGQYERDQLMLEQYDRRVKGLEGELQQVIANTNLQSQSKDDMIRSLQEQVNSLKSKYDVLAKLYSQLRQEHLDKLKELKLLKVKAASAQEAIEKREKLERDIKAKNIELADLIRERDRARYDLDKSRGNQKDVIEKLERQLRLTEDKLADAERSKGADLSLLISKHNRELADLEEALKAKQSVLDNYANRSFDDSDLKLKLAEKDDELEILQETVDSMEAALKEMSLNHNEADQALEEQIDEVLKEHLSKLNSIIDSVLESSAERVQESLFEFESPMQAGNQNSTPEYLLSIVEKASTSATDFATSFNNFIIDGPNGDYSGIISTATILSSVIPDVLNNAKGLTRLARDDTVADNIISYARLAAVAVEDFFSGVQSNKLAQLSDEDKTDKVINFNIDVQTHLQSLSQVSEKLTPRAGTHLNGSGDLGDLVDREMARAAEAIESANAKLNELLSRPKDPKFSVFDLKIHEAILAAAIAVTTAIAALIKAASDCQQEIVAQGKGASSRTAFYKKHNRWTEGLISAAKSVAASTNVLIETADGVLSGNHTPEQLIVASNEVASSTAQLVAASRVKASYMSKTQDRLEGASKAVTSACRSLVNQVQDILSKKQQKEENDVDYSKLTPHVLKTTEMEQQVEILKLENSLNSARKRLGEIRKYSYVDDEDEE +>1173061_clib918_MATdc_A|gene_index=1|name=MATA|role=core_MAT +MNSFLETPALILYSRKSQLPKKSEKSENVMKRIFSPDSFPSSTSKFNDPREKKKIMSDFLFYDGANNTFSETQAPPRKRKNSTEDKPIPRPTNSFICARSMLHKMFERLNISTIECSNLMSDIWKNYNDDLFKHYFRYLSFLEACWHTSVYPNYRYNPRKKLPSPKPSSCALKKYSKSIIKKRPVKTRAKSSGSTNMMPIKINSCTPGFSFNTEAPRRNYELEIIISELMKEEQLYFGMPIIETAPTSVNCKPFQIDDFSDNFLAFSYLYDEGMRDMSGAR +>4952_clib122_MATyl_B|gene_index=0|name=apn2|role=flanking_conserved +MRFVTFNICGINNVLSYHPWNEQRSFEHMFAVLKADVICLQETKLQPHLLKREHALVPGYDSYWSFSNTKKGYSGVAVYVKHGIKVLKAEEGLTGQLVSPDSKKGLTYQQLYERQRERDGSSKSCIGAYPEGNPQRLFQVDSEGRSLVLDLGFCVVFGLYCPSTATEGEERDAYRDDYFGLLEERVNLLVEAGREVVVMGDLNVARELYDSAEGMQALHKAKHITLPTLGDKEEGRFVEVFEKLNPGQCKSWREAKVGRRVFHRLVPDLLQDSCRNKHPNRPDMYTCWNVMMNYRPGNFGSRIDYVLASKGLKVEDADILPHLEGSDHCPVYAEVREPDACQEKDNRATQGAANQTKQASSSAPNSFRPKLFHRTNKQFVQTTSVMSMFRAQTPPTKGITKPKAPSKSTAKKQAAISSFFQKPDPEAAVVSTCVSDVHTKPLGQSQSPAVSPNIESTYQGCDAGAHATREGEDTRPQMATRLDLPEPTEYLDIAERVEEAEKVASQWSKIMTKKPAPVCAHNLPAVLRTTRKKGPNQGKHFWVCGQNHSLQLVGDSYKASADPDAKGSQDDTSARCNFFQWK +>4952_clib122_MATyl_B|gene_index=1|name=matb2|role=core_MAT +MKETRDLLFYNENSIDFNFSPEEMDTHQMMCVNNDSQQDTSFLTATTCIFDFQKDIYDDQNLMSFDPFFPQQRDMALWIASVGNGAIDWSTKPQLTLSKKIPDVVNEKGEEITPADLQRHYADKHVAKLPDHSCDLYYLLFKHAKFEATTLRIPLSLLNDNGTGALGPFDEIRSLTDEEQRLLTLLKTISHYSWVTKHATKDCTGQLLDLCKEGLQLVESIQVNKTFNGVILHILVAYFGRRSTALRDEFQLHKLFLEQRLDIELEILAQHGGADAIDSEKLRKVKKLSELWEWFNAIPYNHHPSVSQIEFIASQIGEKVSFVKSWVANKRRTGAKKRSKKPAPSTQIRPALKVFLEKKPFVSRQVV +>4952_clib122_MATyl_B|gene_index=2|name=matb1|role=core_MAT +MRQDEYHIKQRIFSISKAAITMIKIKTEHKVVKARTPKKMKPGMARFRIQAVPIINQNNNAYLSEISFEEKLEPVPPMYPELQKVLDYIHSRKVPFDGSSGPYPPSSRRQVVNGYVAFKLYHHVPHTDLSAIDITHLLQGPWRACPDRHIWTKYADHYRSRGRDVAFKTWLLSVTSPAPEKGLIIQPQTQSEQGYNFSSSSDFSSSPEHSETLPVSSGEPSTPVDPFLIEFDQGIAEKEASFTSPCNQNYDLDFSQLQPLSIRVPYFNQYASSSNSGLDVDYFDGSTLDTPSYIDTFITPLH +>4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved +MSSNRAEVDLNVNIKKATNTDESAPKRKHVRACIVYTHDHRSSKAFWNGIRIQPLQTDDVMVFKALITIHKVLQEGHPSALRDAQANVNFIQSLARGNNYTGPNQGNHGYGKLISEYVRFLMQKLTFHKYHPAFNGMFEYEDYISLRTVNDPNEGYEAIMNLMTLQDGVDDFQRLVFSSLQRSRSNECKISSLVPMIAESYGIYRFVISMLRAMHVSTGNDEALESLRQRFYSQHDRLRDFYYDCSTLKYLRSIVTIPQLGKNPPNLLEEEDGPGLPQRPKSVAETRSVSPEPAPLPVATPTPSIPAEAQPIENFWSQDALLAQQQYDAEQERLRQQQAMEEERIRQMQMQQQQQFEMQQRQQMEAQQRAQEQLMADQMARHAQGRMAELERDILALRGQYDQDQLMLEQYDGRVKALEAELNQLQQTAHQSAQAKDDLIESLQQQITMWRQKYETLAKRYSSMREEYLALLKKLKATQQKAASAKEAIEKAEKLERDMRHKNIELADLIKERDRARYDLDRAKGGNKEDVERLERELRMAQDKLADKDRSTGADLSLLLSKHNRELSELENALKMKQRALDERGDDSDLLRRLEEKEIEYEALNEAFNSLALHQEQQGNNSNGGSTPLAALHSIIDALLESGSQRVQDALFELESPMQAGNQNSTPEYLLSVIEKASESASAFATSFNNFLADETDGDYAEIIKTVNIYSTAVENVLSNSKGLTRLAKDDASADALVNFARESAEATERNFIGLLSEIIEDFPLDDKMERVITLNMNVQTALQHLTKLAERMAPKTNINMSGDLGDLVEREMAKAADAIAAASAKLGDLLKFNQSDPLKSTTDLQLHEAAIQAAQAVINAIAALIRAATDAQNEIVAQGRGTSSRAQFYKKNNKWTEGLISAAKSVAASTNILIEKADGTLRRTSGLEELIVASNNVAASTAQLVAASRVKATFMSKTQDKLEECSKVVTSACRNLVKQVQEILNKKFGELDEKVDYAALSKHEFKTTEMEQQVEILKLENDLQGARKRLGQMRKVAYLHQDEEESIPGQED +>4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved +MSSNRAEVDLNVNIKKATNTDESAPKRKHVRACIVYTHDHRSSKAFWNGIRIQPLQTDDVMVFKALITIHKVLQEGHPSALRDAQANVNFIQSLARGNNYTGPNQGNHGYGKLISEYVRFLMQKLTFHKYHPAFNGMFEYEDYISLRTVNDPNEGYEAIMNLMTLQDGVDDFQRLVFSSLQRSRSNECKISSLVPMIAESYGIYRFVISMLRAMHVSTGNDEALESLRQRFYSQHDRLRDFYYDCSTLKYLRSIVTIPQLGKNPPNLLEEEDGPGLPQRPKSVAETRSVSPEPAPLPVATPTPSIPAEAQPIENFWSQDALLAQQQYDAEQERLRQQQAMEEERIRQMQMQQQQQFEMQQRQQMEAQQRAQEQLMADQMARHAQGRMAELERDILALRGQYDQDQLMLEQYDGRVKALEAELNQLQQTAHQSAQAKDDLIESLQQQITMWRQKYETLAKRYSSMREEYLALLKKLKATQQKAASAKEAIEKAEKMERDMRHKNIELADLIKERDRARYDLDRAKGGNKEDVERLERELRMAQDKLADKDRSTGADLSLLLSKHNRELSELENALKMKQRALDERGDDSDLLRRLEEKEIEYEALNEAFNSLALHQEQQGNNSNGGSTPLAALHSIIDALLESGSQRVQDALFELESPMQAGNQNSTPEYLLSVIEKASESASAFATSFNNFLADETDGDYAEIIKTVNIYSTAVENVLSNSKGLTRLAKDDASADALVNFARESAEATERNFIGLLSEIIEDFPLDDKMERVITLNMNVQTALQHLTKLAERMAPKTNINMSGDLGDLVEREMAKAADAIAAAASAKLGDLLKFNQSDPLKSTTDLQLHEAAIQAAQAVINAIAALIRAATDAQNEIVAQGRGTSSRAQFYKKNNKWTEGLISAAKSVAASTNILIEKADGTLRRTSGLEELIVASNNVAASTAQLVAASRVKATFMSKTQDKLEECSKVVTSACRNLVKQVQEILNKKFGELDEKVDYAALSKHEFKTTEMEQQVEILKLENDLQGARKRLGQMRKVAYLHQDEEESIPGQED +>4952_w29_MATyl_A|gene_index=1|name=mata1|role=core_MAT +MPSRTPTDIWRCQRLILAARKGETTCQALHEQSIEISSSLKWFEEISEWMSKFPESGLYISTTIWTFLEGVPNEHRIKYDRLIRHYITYIQQLVPARLVTAYLDSLFKICSHPTRTERRIIGEHCGISLHQVTQWFTNRRFREPKTDSPDDGSTRFIPSPMTSPGSIDECSFPESP +>4952_w29_MATyl_A|gene_index=2|name=mata2|role=core_MAT +MENTILHIHSFQLPQTEQPYPEAMLFDRDTSDSRTVLTQKPNGLEISLNFLQYDGHKGLFIRQDSRTNEPEYIEPKVLKPRNSYILFRNATSRCSQSIDPNSAFVSRVSSYIWGSGIPNPIVRRWFKYMGFFEALYHEVDYPEYIPNKLPKSPKKPKVQKGATKSKKKGAKGKNKVTALQVLVGPTVGVGGRMTVSPMTAFFSNNSHVTCYDPNFVLDTPTREFLMMPLDDITLPSQGPRSDSQEQHVPRQPPDGQDYFDILDIDDFVSPYDDLTTRSFTNRQLFKHATLG +>4952_w29_MATyl_A|gene_index=3|name=apn2|role=flanking_conserved +RDAYRDDYFGLLEERVNLLVEAGREVVVMGDLNVARELYDSAEGMQALHKAKHITLPTLGDKEEGRFVEVFEKLNPGQCKSWREAKVGRRVFHRLVPDLLQDSCRNKHPNRPDMYTCWNVMMNYRPGNFGSRIDYVLASKGLKVEDADILPHLEGSDHCPVYAEVREPDACQEKDNRATQGAANQTKQASSSAPNSFRPKLFHRTNKQFVQTTSVMSMFRAQTPPTKGITKPKAPSKSTAKKQAAISSFFQKPDPEAAVVSTCVSDVHTKPLGQSQSPAVSPNIESTYQGCDAGAHATREGEDTRPQMATRLDLPEPTEYLDIAERVEEAEKVASQWSKIMTKKPAPVCAHNLPAVLRTTRKKGPNQGKHFWVCGQNHSLQLVGDSYKASADPDAKGSQDDTSARCNFFQWK diff --git a/results/2026-10-29_ss_records/ss_models.slurm b/results/2026-10-29_ss_records/ss_models.slurm new file mode 100644 index 00000000..fe4105a3 --- /dev/null +++ b/results/2026-10-29_ss_records/ss_models.slurm @@ -0,0 +1,15 @@ +#!/bin/bash +#SBATCH -p exfab -A exfab -c 8 --mem 24G -t 04:00:00 -J ss-stringtie +#SBATCH -o /bigdata/stajichlab/jstajich/projects/MATPredict/logs/ss_stringtie_%j.log +# Transcript models (StringTie) and ORFs (TransDecoder) from the merged S. schoenii RNA-seq alignment (non-stranded library). +set -euo pipefail +W=/bigdata/stajichlab/jstajich/projects/MATPredict/results/2026-10-29_ss_records/work; cd $W +set +eu; source /etc/profile 2>/dev/null; module load stringtie/3.0.3 transdecoder/5.5.0 gffread/0.12.7; set -eu +stringtie all.bam -p 8 -o stringtie.gtf -l SS +gffread -g genome.fa -w transcripts.fa stringtie.gtf +gffread stringtie.gtf -T -o /dev/null 2>/dev/null || true +rm -rf td; mkdir td; cd td +TransDecoder.LongOrfs -t ../transcripts.fa -m 60 > /dev/null +TransDecoder.Predict -t ../transcripts.fa --single_best_only > /dev/null +cp transcripts.fa.transdecoder.pep transcripts.fa.transdecoder.gff3 .. 2>/dev/null || cp *.transdecoder.* .. +touch $W/../DONE2 diff --git a/results/2026-10-29_ss_records/ss_rnaseq.slurm b/results/2026-10-29_ss_records/ss_rnaseq.slurm new file mode 100644 index 00000000..0337408c --- /dev/null +++ b/results/2026-10-29_ss_records/ss_rnaseq.slurm @@ -0,0 +1,29 @@ +#!/bin/bash +#SBATCH -p exfab -A exfab -c 8 --mem 32G -t 08:00:00 -J ss-models +#SBATCH -o /bigdata/stajichlab/jstajich/projects/MATPredict/logs/ss_models_%j.log +# S. schoenii (GCA_054953955.1, strain GDD12): public RNA-seq (PRJNA1364152) mapped to the assembly, and the curated +# Saccharomycotina MAT proteins aligned with miniprot, to build gene models for the MAT loci (the paper's GTF is not public). +set -euo pipefail +D=/bigdata/stajichlab/jstajich/projects/MATPredict/results/2026-10-29_ss_records +W=$D/work; mkdir -p $W; cd $W +set +eu; source /etc/profile 2>/dev/null; module load hisat2/2.2.1 samtools/1.19.2; set -eu +export PATH=/bigdata/stajichlab/jstajich/projects/MATPredict/.pixi/envs/default/bin:$PATH +ASM=https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/054/953/955/GCA_054953955.1_ASM5495395v1/GCA_054953955.1_ASM5495395v1_genomic.fna.gz +[ -s genome.fa ] || curl -sL $ASM | zcat > genome.fa +samtools faidx genome.fa +miniprot -t8 -d genome.mpi genome.fa +miniprot -t8 --gff -P SS genome.mpi $D/refs.faa > miniprot_all.gff 2> miniprot.log +[ -s idx.1.ht2 ] || hisat2-build -p 8 -q genome.fa idx > /dev/null +for RUN in SRR37453601 SRR37453602 SRR37453603; do + P=${RUN:0:6}; L=00${RUN: -1} + for m in 1 2; do curl -sL --retry 5 --retry-all-errors -C - ftp.sra.ebi.ac.uk/vol1/fastq/$P/$L/$RUN/${RUN}_$m.fastq.gz -o ${RUN}_$m.fastq.gz; done + [ -s $RUN.bam ] || hisat2 --dta -p 8 --max-intronlen 5000 --summary-file $RUN.hisat2.txt -x idx -1 ${RUN}_1.fastq.gz -2 ${RUN}_2.fastq.gz | samtools sort -@4 -o $RUN.bam - + samtools index $RUN.bam +done +samtools merge -f -@4 all.bam SRR3745360?.bam; samtools index all.bam +# per-base depth and spliced-read junctions over every contig carrying an MAT call +for R in CM143126.1 CM143127.1 CM143128.1 CM143129.1 CM143130.1 CM143131.1; do + samtools depth -a -r $R all.bam | awk -v r=$R '$3>0' > depth_$R.tsv + samtools view all.bam $R | awk '$6 ~ /N/{print $3,$4,$6}' > spliced_$R.txt +done +touch $D/DONE diff --git a/results/2026-10-30_saccharomycopsis/44080_gdd12_MATss_MATa.yaml b/results/2026-10-30_saccharomycopsis/44080_gdd12_MATss_MATa.yaml new file mode 100644 index 00000000..81fdfecc --- /dev/null +++ b/results/2026-10-30_saccharomycopsis/44080_gdd12_MATss_MATa.yaml @@ -0,0 +1,126 @@ +record_id: 44080_gdd12_MATss_MATa +record_version: 1 +taxonomy: + taxid: 44080 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Ascoideales;f__Saccharomycopsidaceae;g__Saccharomycopsis;s__Saccharomycopsis_schoenii + lineage_resolved_date: '2026-10-30' +organism: + species: Saccharomycopsis schoenii + strain: + name: GDD12 + known: true + culture_collection_ids: [] + differs_from_sequenced: false +mating_type: + locus_name: MATss + idiomorphs: + - MATa + system: homothallic +locus: + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + core: + completeness: partial + reference_orientation: sla2->MATa1 + definition_note: 'Right (palindromic) cassette of the active MAT locus on chromosome + IV of S. schoenii GDD12 (CM143129.1; the paper''s span 528,044-537,777; an SLA2 + anchor between mirrored MAT arrays, Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC + (Dost et al. 2024, PMID 38492364). The record covers the arm that RNA-seq resolves: + SLA2 533,981-535,828 (plus, 615 aa) and the homeodomain gene named MATa1 after + the paper at 536,046-536,944 (minus, 211 aa; introns of 205 bp, 221 reads, and + 58 bp, 218 reads, the conserved 58 bp junction the paper reports at 536,635). + The mirrored arm (SLA2 529,992-532,490; homeodomain ORF 529,258-529,749, 163 + aa) is not recorded: short reads cannot separate the two inverted copies, and + the paper''s mirrored junction at 529,128 is not seen. No HMG-box (MATa2) ORF + passed the filters. GENE MODELS ARE OURS (tier 3), see results/2026-10-29_ss_records/README.md. + The library is not stranded. GENETIC CODE: NCBI table 12 (CUG = Ser).' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: CM143129.1 + seq_region: CM143129.1 + start: 533981 + end: 536944 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] + assembly_accession: GCA_054953955.1 + assembly_accession_basis: strain_match_verified + assembly_location: + contig: CM143129.1 + start: 533981 + end: 536944 + identity: 100.0 +genes: +- gene_index: 0 + name: sla2 + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 533981 + end: 535828 + strand: + + order_in_locus: 0 + transl_table: 12 + exons: + - start: 533981 + end: 535828 +- gene_index: 1 + name: MATa1 + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 536046 + end: 536944 + strand: '-' + order_in_locus: 1 + transl_table: 12 + exons: + - start: 536693 + end: 536944 + - start: 536296 + end: 536634 + - start: 536046 + end: 536090 +evidence: + locus_existence: + tier: 2 + experimental_method: published description of the SLA2-MAT cassettes and RNA-seq + of the active chromosome IV locus (Kriti et al. 2026) + citations: &id001 + - pmid: '41849659' + doi: 10.1093/g3journal/jkag067 + - pmid: '38492364' + doi: 10.1016/j.micres.2024.127691 + boundaries: + tier: 3 + experimental_method: OUR OWN gene models from the public RNA-seq (PRJNA1364152; + StringTie, TransDecoder, phmmer/Pfam) because the authors' GTF is not public; + coordinates are ours, the locus span is the paper's + citations: *id001 + idiomorph_assignment: + tier: 3 + experimental_method: the paper's assignment of the sub-cluster (alpha-dominant + left, palindromic a right); gene names follow it by position; phmmer ranks both + homeodomain ORFs closest to MATa1-type references, so MATalpha2 is a hypothesis + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: '' + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: 41849659|44080|MATss|MATa + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/results/2026-10-30_saccharomycopsis/44080_gdd12_MATss_MATalpha.yaml b/results/2026-10-30_saccharomycopsis/44080_gdd12_MATss_MATalpha.yaml new file mode 100644 index 00000000..691e9645 --- /dev/null +++ b/results/2026-10-30_saccharomycopsis/44080_gdd12_MATss_MATalpha.yaml @@ -0,0 +1,144 @@ +record_id: 44080_gdd12_MATss_MATalpha +record_version: 1 +taxonomy: + taxid: 44080 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Ascoideales;f__Saccharomycopsidaceae;g__Saccharomycopsis;s__Saccharomycopsis_schoenii + lineage_resolved_date: '2026-10-30' +organism: + species: Saccharomycopsis schoenii + strain: + name: GDD12 + known: true + culture_collection_ids: [] + differs_from_sequenced: false +mating_type: + locus_name: MATss + idiomorphs: + - MATalpha + system: homothallic +locus: + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + core: + completeness: partial + reference_orientation: MATalpha1->MATalpha2->sla2 + definition_note: 'Left (alpha-dominant) sub-cluster of the active MAT locus on + chromosome IV of S. schoenii GDD12 (CM143129.1; the paper''s span 470,610-475,000, + Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC (Dost et al. 2024, PMID 38492364); + one of several SLA2-MAT cassettes (one per chromosome plus a palindromic one + on chromosome IV). GENE MODELS ARE OURS (tier 3): the BRAKER2 GTF the paper + cites is not on its GitHub or Zenodo, so the models were rebuilt from the three + public paired RNA-seq runs (PRJNA1364152; hisat2, StringTie, TransDecoder, phmmer + against the curated MAT proteins, Pfam-A); see results/2026-10-29_ss_records/README.md. + The library is not stranded; strand is the ORF''s. MATalpha1 is single-exon, + minus strand, 191 aa. The homeodomain ORF (named MATalpha2 after the paper) + is partial (103 aa, two exons with a 217 bp intron of 198 reads that joins it + toward SLA2, the paper''s MAT-SLA2 splice junction); phmmer ranks it closest + to MATa1-type references (50 bits to C. albicans MTLA1), so the name is a hypothesis. + SLA2 here is a diverged copy: 256 aa (472,708-473,478, minus); a second SLA2 + piece (473,680-474,606, 309 aa) is not recorded. An HMG-like ORF at 470,349-470,951 + (200 aa, 44 bits to S. cerevisiae MATA2, no Pfam hit) is not assigned to a gene. + GENETIC CODE: NCBI table 12 (CUG = Ser) for the species; proteins are translated + with it.' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: CM143129.1 + seq_region: CM143129.1 + start: 471020 + end: 473478 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] + assembly_accession: GCA_054953955.1 + assembly_accession_basis: strain_match_verified + assembly_location: + contig: CM143129.1 + start: 471020 + end: 473478 + identity: 100.0 +genes: +- gene_index: 0 + name: MATalpha1 + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 471020 + end: 471595 + strand: '-' + order_in_locus: 0 + transl_table: 12 + exons: + - start: 471020 + end: 471595 +- gene_index: 1 + name: MATalpha2 + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 471961 + end: 472489 + strand: + + order_in_locus: 1 + transl_table: 12 + exons: + - start: 471961 + end: 472227 + - start: 472445 + end: 472489 +- gene_index: 2 + name: sla2 + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 472708 + end: 473478 + strand: '-' + order_in_locus: 2 + transl_table: 12 + exons: + - start: 472708 + end: 473478 +evidence: + locus_existence: + tier: 2 + experimental_method: published description of the SLA2-MAT cassettes and RNA-seq + of the active chromosome IV locus (Kriti et al. 2026) + citations: &id001 + - pmid: '41849659' + doi: 10.1093/g3journal/jkag067 + - pmid: '38492364' + doi: 10.1016/j.micres.2024.127691 + boundaries: + tier: 3 + experimental_method: OUR OWN gene models from the public RNA-seq (PRJNA1364152; + StringTie, TransDecoder, phmmer/Pfam) because the authors' GTF is not public; + coordinates are ours, the locus span is the paper's + citations: *id001 + idiomorph_assignment: + tier: 3 + experimental_method: the paper's assignment of the sub-cluster (alpha-dominant + left, palindromic a right); gene names follow it by position; phmmer ranks both + homeodomain ORFs closest to MATa1-type references, so MATalpha2 is a hypothesis + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: '' + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: 41849659|44080|MATss|MATalpha + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/results/2026-10-30_saccharomycopsis/README.md b/results/2026-10-30_saccharomycopsis/README.md new file mode 100644 index 00000000..ebc57009 --- /dev/null +++ b/results/2026-10-30_saccharomycopsis/README.md @@ -0,0 +1,15 @@ +# Saccharomycopsis schoenii: MATss family and two candidate records (2026-10-30) + +Curator decisions (2026-10-30): draft records from our own gene models (tier 3); a scope-only family per genus, as for MATkp, MATog and MATpk. + +- **Family** `MATss` (scope: genus *Saccharomycopsis*, taxid 4943; scope-only, never searched on a phylum fallback) in `db/Ascomycota/order.yml`. +- **Records** (candidates, `needs_review`): `44080_gdd12_MATss_MATalpha` (left sub-cluster: MATalpha1, MATalpha2, sla2) and `44080_gdd12_MATss_MATa` (right palindromic cassette: sla2, MATa1). Homothallic. Strain GDD12, assembly GCA_054953955.1, chromosome IV CM143129.1. +- **Evidence:** Kriti et al., G3 2026 ([10.1093/g3journal/jkag067](https://doi.org/10.1093/g3journal/jkag067), PMID 41849659) for the SLA2-MAT architecture and locus spans; Dost et al., Microbiol Res 2024 ([10.1016/j.micres.2024.127691](https://doi.org/10.1016/j.micres.2024.127691), PMID 38492364) for homothallism. **Gene coordinates are ours**: the authors' GTF is not public. Models, method and limits: `../2026-10-29_ss_records/README.md`. +- **Genetic code:** NCBI table 12 (CUG = Ser); every gene carries `transl_table: 12`. +- **Checks:** `make_records.py` re-extracts every CDS from the window fasta (stop codon, no internal stop, expected length); `curate-db propose` passed; `curate-db validate` under Slurm (`validate.slurm`, job 31445666) gave `taxonomy_current` true. No protein accessions, so no per-gene sequence match. + +## Open points for the curator +- Gene naming: both homeodomain ORFs are closest to MATa1-type references (phmmer 50 and 47 bits to *C. albicans* MTLA1); the left one is named MATalpha2 after the paper by position only. An HMG-like ORF (470,349-470,951) is not assigned. No MATa2 in the right cassette. +- Both SLA2 ORFs lack a start codon (5-prime partial). The mirrored arm of the palindrome is not recorded. +- The label registry has no entry for `Ascomycota:MATss`; a system code needs your approval before the records are labelled (frozen codes). +- Scope is the genus; *S. lassenensis* is heterothallic (Dost et al.), so narrow to the species (44080) if a replay over-calls. diff --git a/results/2026-10-30_saccharomycopsis/chrIV_463k-542k.fa b/results/2026-10-30_saccharomycopsis/chrIV_463k-542k.fa new file mode 100644 index 00000000..9a74db32 --- /dev/null +++ b/results/2026-10-30_saccharomycopsis/chrIV_463k-542k.fa @@ -0,0 +1,989 @@ +>CM143129.1:463000-542000 +CTAGGTGCTGCTAAGAAGTTTCTTGGAATGAAGATCAGTCAGTCCTCCACAGGTATACGTCTTGCACTTTGTAATTACAT +CTGCCTGATGGTTGAATAATTCAACATGCAAATCTGTAGAGATGAAGCTACATCTACAGTTTTTGACCAGGACTTGAGTG +CCATTGATGGAAACCTTAAGAGCGCAGATGAAACCCAGTACAAATCCATAGTTGCTGAGTTGTTGTTTGCCGCTAAGATA +GTCAGAGCTGACATCGCATACAATGTTGTGCTCTTTCCAGATATCTCAAAGAACCAAAGAAATACACATGAAGGCTGATA +AAAGAGTTCCCGGATATTTGAAATCGACTACTGACGTGTGTATCTGCTACCGATTGGATGACCCAAACTGTGCGATTTTA +GGCTACTGTGATTCTGTGGCAAATGACCAGATATCCAGAAAGTCCACTACTGGCtttgttttcaattatACTAGCTGCGC +TCTCACCTGGAGATCTAAGAAACAGCCAACCGCGGCGTCGATCATAGTGCAGACATAATACATGGCTGAGTGTGAAGGGA +CTAAGGAAGGTTTTGCTTGAAGCAGTTGATGAACGAGTTTAGTGTCAAGACCGAGCATGTCAAAGTATACAACAATAATC +AGAGCTATCATAACAGTGCGATACATCATGTCTACCCCACAGAACAAACTACATTTACACCTGCCTCAGCTTCATCCGGG +ACCATATTGCTAATGAGGGCATCCAACTGCAATATCTATCCACAGGAGTTATGATAGCAGACATCTTCACACAAGCAATC +CGATCCGCCATATAAGGTGCTTAGAGACTATGCAGGAATGGAAGGCTTTGAATTCAGAGAGGATGTTGAAGTTTGGATTA +GCTGCAGCATCTCATATGCATATTCTCGCATGTGGAATGGTCCTTCAAGAAGGTGTGCTACAAGAGAATgtttcattgaa +aaaaaatacatactaagaaatataaataggGGAGCCCCCTCCCCCAATGTTGAAAGTTCATTctctaatttattatttgc +ttaatattttatataaaaCTAGTATtaatacattttttttccattattattaatattgattgTTTTCCTTCATTTAACTT +GAGTATTAATCAATCAATTATTAAGACAGAAATTGCTTGAATGCTGCAATTACAATAGTTCTCGTCAAACATAACTTGGG +CTGAGACAACAAGCTTGTGGGCAGTAGGATTGAGCATTGTACATGCCTTGTGGTCTGGATCGTAACCCATCATGACACAT +TTCTTGGACGTAGCACCAAGCTTAACCATCACGTAGAGCAGGTGGAATAAGACAGTAGCCAACACAGCCAAAGGTTCTGA +GATAGTCGAGTCTGGTAGTAAGACCAATCCAGTATGTGAAAGGAAGATGGAACTCAATAACCCTGAAAGGTGTTTTGTTG +ATCAAGTAAGCAACAGTCCGGACCGCCTCAGGTCACAACTGGGCAGGCACCGAGACATGAAGCATGAGTATTCTGGTTAA +GTGGTGGCAACGCAGCATTGACAAACTCACCACTATTATCGGTTCTGAGAGTGCCAACCAGTATTTTTTGCCCGTGCTTT +GCAACATGGTTTCGTTGTAGCTCACAAAGTCAATGATGCCCGATTTGTTCTTCAAGCAATGGACTGTGACATAGCGGACT +TTCCATCAACAAAGGTCAAGAAATAGCGGGAAGAGCTAACAGTAGCATCATAGTTCAACAGCCCACCGACATCAATGTGC +AACAATTGTGGTGGTTGAAACAGACGAAGACCACCAGTTCTTTGGCTTGCTTATAGTTCTCTTGCCAAGAGCACATGAGG +GACACATTTCAATCTTGAAGTCGTCGGTGACACTATCAACCGGATAGAATTGGTGGCGGTCTTTTTGAAGACACCGAATT +TGGGATGTCCCAAACGTATATGCCAGACATTCCTAGCAACAGTGGAAAAGGCAGGAAAGATAGGGAAAGCAGTCGAGCGA +GGTATCAACAACAGAAATAGTAGTAGATGGAATGGTTTCAACATTGTAGAGGCCACTGGAATTAGTATCTTTGACTGCAA +CAATTTTCATAGACGACGCTGTTTGATATAGTCAGTCAAACAAGAACTGCACTCTAATGGTGGTAGCTTTTGAAATAGAG +ATCAAGTTAACAGTGGCATCGAAACATACAAAACGTTTGCCAAACAACTGGTCTCTCCATCGGCATCTTCGCAAGAGCAA +TATCCTTACCAACAGTGGTAATAGAAGCACCACCAATACCGTTGATGTCACCCGAGACTCAGACAACTCAGTGAATAACA +TCTGATCACAAATAATATAGCTGGAAGCACCACTATCGAAAAACAAGTTGGTATGATCTTTGACATTTTCAGATCCCATG +TATGCAACCCAAACAGAATTAGAATTAGCATTAGAATCATTTTGTTTTCCATCAGTATTGGTTACTGTGTTTTTTGGGTC +TGTTTGAATCATTGGTGGAGCGATTCTTGTTGCTGAACTTCTTTCGAATGTCAGCATTGTATTTGTTGGTTGGTGGGTCA +GGACAGTATCTCTAAGTATGACCAGGTCTGCCACGGCTGTAGCAGATGAAAGACCTGATTTTCTGTGAGAAGCAGCGAAG +CCCACTTCAGAAGTAGCAGTAGATGAGGTGGAACCAACAGAAGTTTTAATCAAATCTCGGCAATATTTGCGATAACATTA +ATGTTGAACATTCGTATATTGTTAGCATGGGTAGTGTGGATCGTGCTaacaatttctttgatagtAGATTGTTCGTATCC +GGCTCGTTTAATGCATATCAAAAACAATGAAGCAACAAATGTGGAGTGGTCAACACATTAATCACAGCAATTTTAAAAGA +GGAAAATGTATCAATCAAATTTGCGATGTTGCCACTGAAGGTCTTCCATTTGTGTCAAACAAGTAAACCATGTTGTCAAC +AATGTACATGACAGTCATTGTACCAATATGAGCCTGTTAAATAGAGGAACAGCTCTCTAACCGTAGCTTCAAACAATAAT +GCTTTCTTTAAAGCACCATCGGAAACACTGTTCAATATAATGAAAGTCAAAATGTAGTTCAAAGCGACAGTTAGTTATGC +CCGGGTGGTAGCGTCACACCAGCAGTAGCACTAATATCACCTAATTCCAAGTAGGTGAACAAGCCTAAGCttcattttaa +tttcaaagaaaggATCATCCTAATTTTATAAgtataaatagaaaaatagaaaCGAGAATGACCTCCTTAGGAAGGAACGG +TTGAGAATTCTGAATTTAATTTGCATGTGGCAAAGTAAAAACCGTCAAAATGCTTTGTCGTGTGAATTTAACTGATGATC +CTTATTGAGAGCTTGCTTAAATTAACTGCGTATTTTGCGTGTTTAATACAACATCTTATCTATCATCAAGATTATGTAAG +ATTCCTTAGAGCGATTATACGGGAGCCAGATTCCTCCAAGGCTGAAACTacattttaatttcatttataCATTATAATAC +AAAGTGCAAAAGTGCAAGCACAATAACACcgttatttatttcaaattgagTTCTTTTAGGTCAGTCCTGCAACTGCTTTT +GTATTTACTGATAAGATTACTGTGATATATAGtttgttcttttatttttcaatttggCTGCATGTTTAGGTTGTGTGATT +AAGTAGTCATGAACATAAAATGTGTGTCACTTCCCAAACTTCAACCAACACAAAAAATGGagatatttttctttcctaTT +AACCTTACTTTCTTTCGTGAATCCGCCGATTGTGGATTTGAGTATAACAGTAATGCAACACTTTGATTCTCTTAATTCTG +CACTGTTATGCACTATGTGGTTTATATAGCATTATGAACTGACTACGAAATATTTTACGGTTGATTTACTCTgatgtttt +atttttgattgcTTCAGCCTCCTTGGTTTTGGCATATAAGTGCCTATTCTTGTTGTACTGCCCGACTAATCAGactttaa +attttctttaccTGTGGCTTTACATTAGTTTCTGTATTTATATCATCTCCGTGGTAATTAAGCAAGTTATCGGAAGCAAT +AACATATTTTTAACGATAACCGTAAACATTTGGTTCTTTTGACAAACATTCAACTTCACCTGATGGCGATGTGGAATGCT +ACGTACTTTTTGCCGCATTTATTACGTTTTGACGGTTAATAGTTTAGTTGATACTCAATCACTAGATATATCTTGTTGAA +ATTCCAATAACCAAATTCACAGGTTTTCTGTGAAATACAGTGTTTACTGAAGTCTCACTTTTAATGGCTTATCATAGTTA +TagtattaaaaattatatgatCCTTCAGAATATAGCCTCTCAAACAGGAATGCGCATTAAGCTTTATATTACCAGTTGGC +ATATTTCGTAGTTGTTGTTAACCACTTCCATTGAACATTAtgcttcaaaaaaaaaaaaaaaaagaaggttTTACTTACCT +CTAAAGAAATAGTGAATGTAGTTTAGCCAAATTTtagattgaaaaaaaaagcatgTTTTTTAGTTCAGCTCAGCCTTTAA +ACTTGGTAGCAATAAACAGTAGCTGGAATTATAGAGGATGAAAAACTTTTTAAAGTTATGTGAGCCAATTCTTAATAAAG +AGTTTTGTGAAGAAGTGCTGGTGTTtccgatgatttggttaggaTCATCTGCTCTAGTAGAAACACTACTTTTGCtttca +tcaaaaataacagtatcgtcttttttagataaatttagagcATCTAAATAGTTTAAGATTCTGTCGAATTTCTCGTTAAA +TCCGAGTTCTAATGATCTAAACCTTTCTTCGAATTTCTCTTCCAATTGAACTATTGTATCAGAATGACTTGACCGATTGG +ACttgaataaattcaaactaTCATTGTCCGAGTCGCTTTTCATCTTAACTTTGGTAGGAGTCATTTTCAGATTATTGTAA +TCCtaatttaatgaatactgtaagttcttttgtaatgattaatgaaatagttaatTTAAGTATTAAGTAATGTagttctc +ttttttttttcttctatcttcttgatattattcgtattattgttattggatttttcacttgttatattctaattttttcg +tcGTGAAACGTAATATTCACCTGCTTTCTATTGGTCTCATACTTGcatattattactattatattCTTCACATTGTTTTT +CTTAGGATATGGCTATTATATCTTGAGAAGTTTGACCGGCAGTTTTCGGATGCACTTACAGACATTCTACCGGCGTTCAT +CAGCGAAAAAACTGTGAAAATCCATTGTTATTCGGATTGGtaatcatttattattacttattACCAGTTATACCCTAGTT +GTGACTGTGGTTTTTGCTATATTTTTGTCACCCACAACTCATATATAAAGGGTTGCAATTCCCACCTTTCagtatttatt +ataatcttAGTTAATTAGTTTTTATATAGTTAATATAAGATAATCGTTATTTATTGGAATTACTGTAACTCCTACAAAAA +CTGGAGAAAAATCATATGAATATTACACGGAAACGGTATTTGAAAAGCTAGAGGGCGACATAGTGGTGCTAGGAGGCTAT +CGGGGGTCTGTTTTAAGAGACACAGAAACAGGGCGAATGCTTTGGATCCCGATGAAGGTGGGGCTCAACTTGAGAAAGGT +GGATTTGGTGGTGCCTCCGGATGACCAAGCTGAGTATGACATGCCTAAAATTATATACCCTGATGGGATGTTGACCAATA +TAGGGCCTATAGATATCTCGAAGAAACTCATCAAGAAGATGGCGGCCGTGCCCGGGGTACGAGTACATGAATTTGGGTAT +GATTGGAGATTGTCTTGTGATATCAATTCAGCAAAGCTTGAGGCGTTCCTTAAAGAGCTACCatgcaacaacaacaacaa +caacaacaacgacaacaacaacaacaataataataataataataataataataataataataacagtaataataatacgA +ACGGTGAAAAGggcaaaaaaaacaaaggaGCGTTGGTTGTGGCACATTCAATGGGGGGATTAATTGCACATCATGCAATG +AACAAAGATCCAACGCTTTTCAATGGTATTTTATATGTGGGGACCCCTAGTGAATGTCTTAACATTCTTGGTCCTATAAG +ATTCGGGGACTCTGTATTACTCAGTAAGAATGTTTTGACAGCTGAAGCGAATTTCCTGATGCGTTCAAGCTTTGTGTTCT +TGCCTAGACATGGAAatgttttctttgataCAAACACGGAAAAATTCATACATATGGATTTGTTCAACCCTGATACTTGG +GTCAAATATAACTTGTCTCCATTAGTATCTAACTCACACAAAGGCATGCAATCTTATGAATACCTTTCGCGTACATTACA +AAGAACTAAAAAATTTCTCGACGAGTTAAGTTACAATCCagaattgaaagataaGTATCCACCATTAGCTTTAGTTTACG +GAAATACTGTCCCTTCAGTGCGAGGTGTGTTGGTCGATGGTGAGAAGGACATTAAAGATGGTCAATACGATAATTACTTT +TATGGACCTGGTGATGGCGTTATTCATTCAAAATGGTTGATGCCTGAAAATTATGAAGGTTTTAAAACTGTAGCTAAAGT +TCCTAGTGATTGTGGTCATGTTAGTTTAATGACTGATTTTGAAGCTGTTGGAAAAGCTTTGGATGCTATTATTCACCATC +CAAAAGCCTTGGATGCtgttcatcatcattaaataaatacatgtatatatatataaaaaaaatagatatatatatatcta +taacaggatagatatatatatatctattttttttgatctatattttttttttgatctaTACCTTTTTTGATCTGTTTTTT +TGGAGTGCAAAATGTTGTAGCCATTCCTAGAAGGTTCGCACGcaagaagaaaagtatGATTTATATTCTGATTTCCCAAT +GTGGACAAATGAATGGAAACAAGAAGTAGacaaagagaaagaaagaaagaaagaaagagaaacaaAGAGAGAGAGAGAAT +GGTCTCTATATAAGAAAAGTAGTTTCCACctatttacaaaaaaaaataaaaacaaaaaaaaccaaaaaaaaaaaccaaaa +aagaatcatcatcatcatcatcatcactatcatttttcttcattaacaaaaaaagaatgagtgttttgaaaatatcagaG +ATTGTTTCGCCAAACCCAAAAGAAGATCAACTCTTTAACAAAAAGTATGTCATATTAAAaggggaaaaaaaaaaaaaaaa +aaaaatgtatattcttttatttttcctcCATTGCTAGctaacatatatatatatagaataAATAAGATAATGGAATCACT +TTCCAACGTATACAATAGCCTTTTGTCGGAGCTCACGGTTGATTCTATTGTAAACAACAAGATGGAAGTCAATGCAATCA +AACAAAAGGTTgttaaaatattgaaagaatattcGAAAATATCCTCTCAGAACAGGACATCTTTATTGTCGGTTGCTGAT +TTCTGTGATATCTTGATCCAACTAATTGGCGAAAGAtataatcttttgaatGACATTTTTTACTGTCAAAATAGAATTAC +TAGGACGTTATCAAACGTTCCTGTTGTTAAAAACGACGAGCCTGCAGAGGAAGTCTCATTCAACATAGTGACACAGTCAC +AAATAAAACGTAGTGACTACAAGGCCTTACGGAAATGCCGTCATTTCAATACTGAAACCAGACGGAAGCTTGAACAATGG +TACAAGCTAAGTGACTTTTCAAAGGAAGCAATTGAAAAGTTTCTGAATGAAGTCAATTTGGACGTTGTTCAGATCAAGAA +CTGGGTTTCgaataagaaaagaaaagaaaaaaaagctatAAGCAAGGGTATTATCGATGTTCTGAAGACTTTTAGTCCAA +GCAATGTATACGTCATGACTGGggatttataaatattttctttctttcgtttatttatttatttatttataataaatata +tataagtatttaattcatttttatgaaACCACATATTTTCCTTCTATATCACAGATGAAATTGAGAACTTCAAAATCTAG +GAAAAAGTCACAACCATTTGTAGCTTGTTTATTTACCTTGTCCATTTGACTAGTTTTGACATCAAGCCATTCTAGAAATG +GCATGTTTTCGTCTGACTTATTATACTCAAACGCAAATCTTTTCCATATATCCGCTGACGGGTCATCTTTCCACACTGTT +GCCAACCTTTTAGATACTTCAATCTGGAGAGCTGTATCGTGGATATTTTTAGAATAATATGACCTGTAAGCAATAAAGCC +ATTCAACTTTCTTTTGGTTGGTTTCTTCTGTGccttgtttttctttttttccgtACATTGATCGCTTATTTCATTCATTC +TGGGAGTTTTCTTGAATTCAGTTAATAAATCTTCAAGCTTCAGATAACATTTAGGAATTTTAGGAAGAGAATCGAAATAA +AATGTATTTTGAAATGTAGTAGGATATCTTATTGGTGTTGCACTTTTAACCTTACGAAAAGAATAGTTGGTGAATTTATG +gcattttgttttatagTTCTGCTGTAAGATGcccatttttttctttctggtttttattttctttgcaaaacagtatataa +taaatgcaaatgaatatatttcaaGGAATTTCAAGGAAAAGTACTCATTTATATGTATTTTGTATATACTAAAGCAActa +aaacaataataataatgcacGCATGCATGTCAAAAATAAGTAtaaacttatttttttgcttttcttttaaggGTGCTTAA +TGATGATTAACTTTCCTTCTGGCATccacaagaaaaaaaaaaaagcaaaaaacaCGAACTCAATTTGCATCGGAACTTTA +CTATTTTAGTTATCGGATGCAGCAATAAAGAGTGGATTCAAAGTGTGTGATAAGGATTCAgtttataataaaacagAAAG +GATGTTGATCGTCGACATTTTCTTATCACAATCCACTCTCAAATCCAGCAAATATACAGATAAGTTAAGAAAGTATCTTG +AAGATAGAGGTGTAGATATAGTTGTTTTAATGCATGACCAGCTGCAAACGTCTAATAATTCGAGATACTCTAGGAAAACT +ACTGAATATCTAGAACTTCTTTTCGATACAAATCCTAGACCTAATTCTCGTGAAAGATTATTCATAGCGAAGCAATGTGG +ATTAAGTGCCAAACAAGTCAGAATATGGGTGagtattttattattattattattattattattattattcttcttcttct +tcttcttgttgttgttgttttatacaaatatatatatatatatatatatatatatatatatatatatattatttgtgTGG +GACGGGGGTGGAGGAGGCAGCAGCAACCtgaaatttatatttttatttggcAAGCAATATCTAGAACTAACAAGTATTTT +TATAGTTTACCAACAGACGTGCTAGGGTCAAAAAGACACAAAAGCcatagaataataatttattattacgtaaatatata +tatataaagagaaagagagagaataaataataaactttaatctttgtttttcttgtttttcttgtttttttttttttttt +tttttgttttttttaatcGTATGttgtaatatttctttctttaaataaattttttattgagTGATATACTGGAGGAGTAA +GAGtgtaattttaatttaattaatatatctaatattcttcatcattatcgctatcatcatcatcatcttgataactatat +tttctaatatcaCCTAATCTCTTTCTAGCAGCACCTAAGGCATTCTCCAACTTGATGATTTCTGCTTGttgtttcatttc +ttcttttctattatcAATGATACTCAACTTGGagaaatcaatatcatcattaacTAATTGTGTagatttattaatcaatt +ctTGCACTTGGCTTACTAAGCTCTTGCAAGCAATAGACACCTTCTTACTTGCATCTTCCAAATTGTCTTGAGTCTTGGAG +AACTGCGTAGCTTTAACTCTTGCAGCTGCCACTAGCTGAATAGTAGAAGATGCAACTTCATTAGATGCCACAATCAATTC +CTCGTGTGTATTCTTACCACTCAAATTACCATCCGCAGTTTGAATTAATAAGTTGGTAGCAGATGCAACAGCTTTAGCAG +CAGAGATTAATCCTTCTGTCCAtctattattctttttgtagAATTGGGCTCTTGTACTTGAACCTTTACCATTTGCAACA +ATCTCATGTTGAGTATCGATGGCTGCAGAGATTAACATCGAAATAGCTTTGGTGATAGCAATGGATGCTGCCAAAATAGC +CTTATTGACTTCTAAGTCAATAGGTGACAAGTCCTGTAAGTTTTTGCTGGTTAGCAACGACGTCAAGTGTAATGTAGCTT +GCTTGATAACCTCCGCAGCAGCGTTCATTTCGTTGTCCACCAAGCTTTCTAACTCCTCTTCTGTTTGTTTTTCGATCTCA +ATGAacttgttgttgctgttgctgctgctacTGCCACCGCCGCCGCCGCCGCCGCCACCGCTCGTAGATGCAATAGTCTC +AATCAATGTAGTCAATTCTTCTAATCTTTCATGGATTTCAACGACACCTTCAATAACTTTGTCTGACTTTTGTTCTTCGC +TCAAGCTTTGTAAATTCTTAGACAATAAATcttccaaaaattcaatgCATGTCTCACCAACCAATCTTGCAGATGATAAT +AGCGAGTTTTCCAAATTCTCTGTCTTGCAAAACTTAATGATGCCTTTAACATTCAACTCAACATCTGTAATACACAGAGT +GAATTCTGAAATAGTAGAAATGATCTCAGCATAATCACCGCTTGGTCCATCAGCGATAAAGTTATTGAAAGCCGTAGCAA +ATTCTTGACTTGTTTCGCCtgctttttcaataatggtTAGCAAGTATGATGGTGATGAGTTTTGATTACCGCCTTGCATC +GAAGAATCAAGCTCGAATAAACTGTCTTCGATTCGTTTCACACCGCTTTGCAAAATGGAATCTATAATAGCAACTAGCTT +CATGATATGTTCTTGTAACACTGTATCAATTTGTTCATCGATAGCTTCTTCGCTTTCCCTTTTAGACTCGGCTAACTGTT +TTAACGCATCATCCATTGTCTGTTGTAAAATTTCGATTTCCTCATCCTTTTCACGCAATGTTTCCTGTAGCTCAGCATTT +GACTCTGGTGACATCTCTGAGATTTGCGattctctttctttcaatttggTTTCAAGTTCTTCCATTTCTTTTCGGTGTGC +GTTGAAAATGGAGTTCAAATTAGACGACTGGTTTCTTTCTGTCACATCCAACTTATCCTTCAAAGTATTAACTTCGGCTT +CTAGTTTTTCAATCAAAGAGTCCTTAGACCCTCTTAACTTGTCAATCTCCAACCTAGCTCTGTCACGTTCTTTAATCAAA +TCTGCCAATTCAACATTCTTCGCCTTCAAATCTTTCTCAATCCTGTCTCTCTTATCAATAGCCTCCTGTGCAGATGCAGC +CTTTTGTTGCaactttttgaatttttgcaataaattcaaatgtTCTTGTCTCAATTGCGAGTACAATTTTGCTATGCTCT +CATATTTGCTCTTGAACTGTGAAAATCCGTTGATATTCGGATTGGTAATCATTTATTACTTATCACCAATCATTCAATAG +TTAATCAAATTATacttatcattattgttattctcTACTTCATCATCCTTACCAATTGTTACTCCAGGAATATTCCAGAA +AGTTCCATAGAATTCCAGAAAGTTCTAGAAGATTCGAAAAGACTATGAAACTTGCATTGTTACAAGAATATTCCTGCACA +TTGTTACATCACTATAAACTACAATATGGCTATTCTCTTATAGTTATGCTTTACTTGTGACTGCTCTATTTTTGCTACCC +ACAACTAATATATAAAGGATTCAATCGGAATGACTGTTTAGGTAACTCGagaataagattgttaaaagtaaatcacttga +aggagcagaagtgattggagtttatttttgtatgtaatagagaaaatatattaaatataaaacattgttgagaataaata +aatgatacaatttgtatatcaataattaataagtaggattcttgattaaaaggaattaaaataaataataaaatgacGAA +ATATATAAGCAGAAAGTGGATTtaggttctttatatataatagtagtttcatttctccttgattatttaatctaatgatt +aaacGGCTTTGTATTAGTAGCATCACGGCACATTCTTACTAATCATAAGGCACAATCTTAGTAATCAGTTGTAAAAAGCC +TCATAGTGTTATCTATAATTCGacataatttatattttttttttttttttgaagtgaaattgaataaactAAACTTATGT +TATTTGACAAGAGATGGCACTAGGCTGGAAAAGGGGATAGTAGTTAATGGTTTGGTAAGGATGTCAGCTAGTTGAGCTTC +CGTTGGGACATGTGCTAAATGGAGTTGTTTCATGTTATAATATTGTCGACAGTAGGCAAGACGAATGTTGAAATGTCTAT +TGCGCTTGTTGTACTGATGTTGTTTGATCATAGTAATTGATGCAGTATTTGTGAAAATCCATTGTTATTCGGATTGGTAA +TCATTTATTACTTATTACCAATCATTCAACagttattcaaattatacttatcattattgttattctcTACTTAATCATTT +TTACCAATTGTTACTCCAGGAATATTCCGGAAAGTTTCATAGAATTCCAGAAAGTTCTAGAAGATTCTAAAAGACTATGA +AACTTACATTGTTACAAGAATATTCCTGCACATCGTTACATCATCATATACTACCATATGGCTATTCTCTTATAGTTATA +CCTTAGTTGTGACTGTGGTTTTTGCTATATTTTTGCCACCCACAACTCATATATAAAGGGTTGCAATTCCCACCTTTCag +tatttattataatcttAGTTAATTAGTTTTATATAGTTATAATAAGATAATCGTTATTCATTGGAATTACTGTAACTCCC +ACAGATTTCCAATCTTTCTCCTATATCTCAACCATAGTTATCCAATCAAATCCTTTGTTATCCTACTTTATTTACTCCAA +TCAAAtcctttattatttactgATTGCTTTTTCCTTTGTTATcctattttatttactccgatcaaattctttattattta +ctgattgtcttttcttttgttatCCTTTGTTATTTAGACACagtttaaaaaaaaaaaaaaaaattattattattatcccT +TTAATATGTCTTATCCAATTCCAGAAGACATATCTTATGACAAAGTCAAGGGTATTATTGCAACCTTGGGACTTAACACC +GATAATCAAGAAGGCAAATATCAAGAAGGATACTTTTATCCGTTAGTTGAACAGTTCATGATTAACAATGTGTCTGATGT +TATAAGAAAggatatatttgatattgaacGTTTTTTTGACTCGAAAATTAACTTTGTGCCAACCAATATATCATTGGTCA +TTAAGAGTGTTCTTAAttctaaattttcaaaagcttTTCTTAATGACTACAAAATCTCTGATGGTGCTAAATTGTTCTTg +gaaattaaaaaagaattcaagGATCTCAAGAtcaataagaaaattaCGTTGCTCAAACACTATCTCAATAATTTTGCAAT +AATCCATGATATTAACCACTTCAATAACTacactattattattgagtACTATTTTCCTGAACTCAAAAACATTGGAAAAT +ACTTGTTATTGTCTCAATTATCAGTGAAAGACATTGGTGTGGTTGCAAAATGTAATCTGATGACTAAACAACTGAGCTAA +ACACAATGCTGATTGATAAGTTGAtaagaaatatcaaagagttatctgaagaagaaaaagaagtttttcaataaaacCGA +AACTATGCTTGTTGAAATGCCAATtgctgatgatgatgaaatttcaaatggtgaatctgatgatgaagcGATGTTTAATC +AAGATGCAAATAAAGAATGTTACTCTATTGCTGCAACTCCAAAGTTTACTTTGGATTCAGGTTCCACTATTCATATAATC +AATGATGaatcttttcttgaaaataaaaaattagataatGGAAAAATTACTACAATCAATGGTTCTACAAAATCTTTGTT +GAAAGGATGTGCTAACATTGGGAAATTGTATCTTGATAATGTAAAATATATTCCCGGCTCTCCTCGAAATTTAATATCAA +TCCCTAAGCTAATTGATGCTGGATTCACCGCTACAATTGACACTGAtacaatgaaaatttttaaagatgGTCAAGTCGTT +GCTTCTGCCATTCGTGATggtaaattttcaaataaggAGATTCAAGAAGCATATGAAGAATGCGAAATTTGTCAAGACAA +AATTGCTAACAAAGCAGGTAAACCATTAAAGAGAGAATATAAAGTTGGTGAATATATTCATGGTGATTTAATTGGCCCAA +TAAAAGACTGTTGGGttcttattattgctgACCGTGCTTCACACTTTGCTTTACATAAAGTGCTTAAGCAAAAATCTCAA +GTCTTTATTGAAACAATCAATATTGTTAAAAAgttcatcaatattttgaaattaaacGGAAAAATGATCACTCATTTTAG +ATCTGATAACGAATTCAGTACTAAGATGTTGGAAGCATTTTTTAATGATCAAGGTATCATATATGAACCTTGCACTCCCC +ACTCCAGTTTCCAGAATGGTGCAAGCGagaatttaaatattttgatccAAATAAAGGCAAAACAAATCATGGCAGGATCA +GACGTTAATGATCTGTTGTGGAATTTTGCAATTGGTCATGCAGTATTTCTTAACAACTACCTTccaagaaatgaaaatag +tCTATCACcttatgaaattattttcaagagAACAAAAAAGACTGGAGACTTAATAAAATCTGGAGCTGACGTGATTTCAT +ACAATTATGATAGAACTGGCAAATTCTTTGTGAGAACTATCAAAGGAAAATTTTTGGGCTATGATAAAACAGACAAAATA +GCGTTTGTTAAAACTCCAAGTGGAAAAATTATCCGTACTTCACAATTTAAggaattcaaaaagaatgatCCTTGGTTTAA +GTTGAATGCAGGTCAAGGTGATGGATTCTCCACTAATTctggtggtggtggtggtaTCGCCAGTTTCGATGGTGCATCTG +GCGATAATGAAACCtcaaaagataaaaatgaacTGGAACCAATGGACGttgatgataaagaatatgaattattagtgcaa +aatgataaaattatcaatgaaaatttgcaattgaagaaaaagatcaaAGAATTTGAGAAACTCCAGgaattgaataaaga +aaCTAATACGAAAATTAGAAATACTGCTAATGcattattcaaagaaaatactaAAAACCAAGAACTAGTTGCTCAAAACA +TCGCTGAAAGAAATCGGCTTATTACTGAAAGAAGTCATATACTTGATGAGAATAGAAAACTAGCATTGATAAACAAAACT +ATTGCCTCCCGTGTTAGAAAAAGAACTCTTTCAGACAGAAGAAAGGAAAAACAGGCTGCTTaagaacttgaaaataaatc +aatacaaaaaaatactgAAGCAAATCCAAACAATCAATTACAATTAGTTccattttctattttctaaTGAAGACGTTATT +GAACTCTCTGATAGTGATATCGATGATTATGTGCCAAAGAAGCTTAGTGTTCAAGACATTTCCAATGACAAATCTCATGG +AATCAAATTGATTGAACCGAGTTCACATCAAAGATTTTTGATTGAAGGAGCTCCTGGTAGTGATTCTGAACGTAATtctg +aatttgaaattgtcAATAATGAAGTATTTTTGTTGGTTGCTAAAAACAAGTACAAGATTCCAACAACATACAAGCAAGCT +CTAAAATCCAAGCAGGCTGATAAATGGAAAATTGccattgaagaagaattgaaccaaatgaaaaatctcGGTGTCtttga +agaagttgatGTTCCAAAAGACAAGAATTTGATCAAAACCAGATATGTGTTCAACGtcaaacaagaaaataaatctgAAC +GTTTTAAAGCCAGACTTGTTGCAAAAGGTTTCCTTCAAATTCCAGGTGAAGACTTCAATGATATATTTAGTCCTGTCGTT +CCATTTGAGGTGATAAGATTGTGTATTGGGTTAGCAGTTAGAAACAACTATGAAATCTGCCAATTGGATGCGAAAAATGC +GTTTTTGAACGCTGACTTGGATGTGCCAATATATTGTGAAATTCCTGAAGGATATGGGTCAAAGCATGAGTCCAAGTGTT +GGCTACTCAAAAAAGCATTATATGGTCTTAAACAGTCACCACGTCAGTGGTTCGCTACTGTTGCTAGTGTCTTAACTGAA +GCTGGTCTTGAAAACAGTATTATTGAGCCATGTCTCTTTTATGATCAAAACAATTTGGTAATCGTTTACGTTGATGATAT +ACCTATTATGTCCAAAaacaatgatgaattgaaCAAAGTGAAAGAAGTTTTCAACAGCAAATATCAAATGAAAGACATGG +GATTTcctaaaatttttcttggtCTCACAATTAAGAGGGGAAAAGATGGCATACAACTTGGTGCTGAAGACTTTATCAAC +AAATTGATTGCTGATTATAGTATTGTTCCACAAGAAAAGTTTATTTCTGTTCCGCTTTCAAAGACGTTCAAATATGATGA +TCCACATGCAAAAGACTTGGATACTGCTCAATACAAGAAGTTTCGATCTGCTATTGGTGTTTTGTTATACCTCGCCAATA +CAGTTAGATATGATATTTGTGTTtacatttcaaaattgtcAAGATACTTGGTTACTCCAAAAACTAATCATCTCAAAGCT +GCTATGagaattattcaatatgtTTGTCAAACCAAAGATTTTATCATCAACTATAAGTCTGGAAGTACAACTAATATTTT +GAACATCAAACCATTTTATGAATACGATAAACATAAACTTGTTGAAAAGCcagatttttcaaagttaaAAAACGACATGA +ATATTACTGTGGTTGTTGATGCTGACTGGGCAAATGACGCCGACAGATACTCTCAAGCTGGTCTAATGGTTATGGTTGAC +AACAATTTAGTGAACTGGAAAAGTTCTAAAAAGAATACGGTGGCTCTTAGTTCAACAGAGGCAGAATACATGGCAATCAC +TGAAGGCTCTAAGTCTGGTAATTACataaaaaatcttttgaatgaattaaaatacAGCATCagttatttcaaattggCCA +GTGATAATATGTCCGCTCTATCATTAGCAAACCACAAGTCAAAGCACAACCGTTCAAAACACATTGATATcaaatatcat +tatattagaaatgaaatttcaagtCTTAAAATTTATCCTTTTTATGTTAATACCAAATTCAACATTGCTGACATGCTTAC +AAAAAACCTTGATGGAATAAGCTTTCAAACCCTCATACCCGTCTTGTGAAATAATCAGGTTTTAGGAGGTGTGTGAAAAT +CCATTGTTATTCGGATTGGTAATCATTTATTACTTATTACCAATCATGCAACagttattcaaattatacttatcattatt +gttattctcTACTTAATCATTTTTACCAATTGTTACTCCAGGAATATTCTAGAATGTTCCATAGGATTCCATAGGATTCC +AGAAAGTTCCATAGGATTCCAGAAAGTTCCATAGGATTCCAGAAAGTTCTAGAAGATTCTAAAAGACTATGAAACTTGCA +TTGTTACAAGAATATTCCAGCACATTGTTACATCATCATATACTACCATATGGCTATTCTCTTATAGTTATACCTTAGTT +GTGACTGTGGGTTTTGCTATATTTTTGCCACCCACAACTCATATATCAGATATAAAGGGTTGCAATTCCCACCTTTCagt +atttattatattcttAGTTAATTAGTTTTTATATAGTTATAATTAGATAATCGTTATTCATTGGAATTACTGTAACTCCT +ACAATGACAGGTAGATTATAAATTCTGTGGAGACGAACGTGTGCGTCATAGAACAAAATCATTGTTAGTAGAATCATTCT +TTTGATGTTGcctatttgtttatttgtAGTATTTCTGTTGTTtgtattgttattattgttggtaAGATGgtcattattgt +tattattgtatgAGGAATTAGTATTGGAATTggaattattgttgttataATTGGAACTAGTGTTGTAATTATAATTGtaa +ttgttgttattataatcggcattattgttgtaattatatttatcattattatggAAGTTTGAATTAATATTGGTATACTG +ggattattattgttatagTTGTGATTGgaattgttgttattattattgcgttatatctattattattgtatctGCTGTTG +TTATATGAGTTATTAATGTTTACAATTctgttattgttttctGCATTTTTTATGTGATTTCTTATGATTTCGGGATATCC +CTCTAAATTGTCTGTTTGGTTGAGGTCACGCATATAAGTTTTTAAGTTGGTTAATGATCCACATAATCCTTTAGTTATGC +TTTAGATTATATCGGCATCAATTAAATGGGTAATCTGATTGTtgtattatagataatactataaggcattttataattga +ttactaagaatgtgtcttatgattagtaagaatgtgccgtgatgctactaatacaaagccgtttaatcattagattaaat +aatcaaggagaaatgaaactattattatataaagaacctaAATCCTCTCTCGGCTTAtacattttatcattttattattt +attatattccTTTAACCAAGGGTCctatttattaattattgatatacaaattgtatcactcatttattctctacagagtt +ttatattcaatattttttctctaaacttgaaaataaattaagtCACTTCTAATCCTACAagtgattttcttttaatacTG +TTTTTCACTTCACTGATACCTGTGAAAATCCATTGTTATTCGGATTTATAATCATTTATTACTTATTGCCAATCATTCAA +TagttattcaaattatacttatcattattgttattctctacttcatcatctttacCAATTATTACTCCAGGAATATTCCA +GCACATTGTTACATTACCATAAACTACAATATGGCTATTCTCTTATAGTTATACTTTAGTTGTGACTGTGATTTTTGCTC +TATTTGCCACCCACAACTAATATATAAAGGGTTGCAATTCCCACCTTTCagtatttattataatcttAGTAAAAAGTTAT +TTTATAGTTATAATAAGATAATCGTTATTCATTGGAATAACTGAATCTCCTACAGAAACTACTATATTATAAACcttaaa +ttattttacttatatcttttaatattttattgcttttttttgtttcctTTTAATTAAGGACATACACATCAATTACTGAT +TCAGATATTttactatttattttatctcaatagagttttatattcaatatattttcttaaaGTTTAAAGATAAACCAAAT +ATGCTCTGATCCTTCAACTAATTTAGTTTAAGACCCATACCCTTAATTACAAAATAAGTAATTACGATTCACTACTACTA +ATTAGTAATTACTAGTTCACTCATAATTATGAAGCTCTAAGTCTGCATGCACCCCGGTTACTTAGACCTGTCTGATAACT +AGAAATGGTGATCAATTACCTCAAAATCTCTACATTTTTTGGgaaagtttcttttttttagttgTATTCTATAAAGTAAG +GCAATTTTAcacttttcaatatatattatatctCAATACTACTAGAGATAAAAatgtatatcaataattaaagGATAAGA +TCATTGATTAAAAGTAATcaaagtaaataataaaagatataAGTGAAAGAGGGATTTGGGTTCTTTTTATGATATAGTAG +TTTCTTTTCAACATATTTGTATTAGTAACAATGCGTCATATAATTACTAATTTCTAGGACATTCTTAGTAAAcatatatc +aaaatcattacATCATTCacattaaagaaaaagcattTTGGCACACGGCGTTATCTAACAGTATCTTAGCCCTACAGATA +GAAAAGTTAAGTGCTAAGTTTTTATGCGCACCTAAAGTTACATATTGATGATACTTTAATCACAGCACACGTAAAATTGT +TGGTAAGCCAATAGTGTCACATGCGTATCACATGATCAAACAGAACCGAACATATATGAATATGGCATTATAAAACTTAA +CATGATGAAACCCATACATGACCTCCTCCACACTTCAAAAAGTCAGATACATGACATTTCATAAAAAATGTATTCTTATC +TTACTACACTTGGGGTGAGATATGATTTATGATTAGCATCGGGTAGTCTGAACTTCTTAGATTTGCTTTTGTCACTAATG +ACTTTACTGGCTTGCAATTACAACGAAGCAATAGTTGCCTTCACACGAAAGATCAATAGTGTTGATCACGTGACATGTAA +ATAGTCTGTCCCTAACCCATGCTAGAATCTTTTTTCTGTAACATCAAGGTCATACTATTTTTATGTGACAAACCGGCTGC +AATTTCAATTTACCATATTATACAATAATACAATAACTTATTACTCATGttccaaatttaaaaatatgtaTTATACGAAA +GGTGGACTACCTTTGTATTGTTCTCACTTCCCATTTCATTCGCGTACATTTACTATGTAGTAAATGTGCTACCCTTGATT +ACAACTTATTGGTGTGTGAACTTTTGGTATAACCTATTTGGTACAGTGATCTAGAATATCGTGTCCggtaaaaattatta +atccGTGTGCATGTACAAACCTGATATTTACATGTCTATTTGCTGATAATGATACGGAATCATGATTATTACTGTGCGAA +AGACTTATTGTTTTTGCATACAGATTTTTATTGTCAATATTATAGTCGCctatttttactttttttttctactaAATAAA +TATGCTTTAATTTAGCTTCTGAACTTTCATCTATGTATTCTATACCTTTTGTAAAATTATcatagaaatattttttaatt +cttttttgatcGCATATGAATACTGCAATTTTTTGAGTCGAAAACtataatttcatttattaagAATTGTCACCTGcgta +tatatttttttaaatttattactagcatatatttttttatttataactGTGGGAACACGATATTGTCAGATCACGTGCAC +AGATATAGCTTAGTCAAAATACGCTATATCAAAGGTGATCATCGTCTACTGTCATGTCCATTTATAAAATAGCAACGCAT +TTAATAAATAGTAACTGTAAGCGATACAAGATGGCTAGTGAGAACGATAATATAAGTAGTCTACATTTCCTCGAgctatt +attaaattatatctCTCACAACAGATGGACAATATAGATACACGTAGATATAGATacatatatcaaaaatataacaacAA +CCTGTCTCTCTCACATAAGTATGAAGAATAACATTAATCAAGAGAGTAACTTAACCCAGAGTTAAGCCAGTCAGACATGA +CTCAACAATAATCACTATCTATTCTTTGTTATATAAGTTTTATATCTAATCTATTCTTTGTTATATAAGTTTTATATCTA +TATAGTAAATCATCGCttattctttcttcaatttttaatttataaaaatatatactCTTTGGCaagtttcaatattatac +CTTTTAAAGGTAGCATAGCTTATTAGtctttaaaaaattctttctaATGGTTTATCGTCATTTCATTTGAGTTTTCtatt +actttatttttattgttaagTTTTTATGTTACGTTGATTATTAGAACTTTTAATAGTTACAGTTCCATCTGCATCAAATA +ATCGTTATTaatctattattaattgacATATGATAATGTTATATTTAGCTTTATCTTTATGGTATATAATACTATCAATT +ATTCTATCGTGCTATTGTACTATCCATAACATATTCTGGTGATTATCGATATATATACTGAGAACCCatatgtatattat +attcCCAAATGTAGGTATATTTAGTGAcatcaaatattaaatgCATATTTGAACTATGACGCTCATTGCTCAAACATAAG +CATCTCCCATATTATCTTTGAGTACATGTATTAtcttatttttgttgaGCCAAAATTATCTACTAAAATACCAATCATTC +AGTAGTGTCAAGCTAGTAGCACTACTCAGGGTAATACTTCCTTAAGATTatataaaatgaaaatacaATCAAGAATAGTG +CGTGCATATAATTTGTTGCATTTTTCATATGGTCGGAAAAGTACAAATGTAGAGCAGGAAAAAGGGGATGGGGGAGATAG +TTACATAGTTGGATTATAAtacataaatatttatctaGAATCGCAATGACTAACAGTAAATTAAACTATGAGGTTATAGA +CTTAAAATAGAACTTAGAGAATCAGATTTCACATACCAGCCGTTCCTCTCTTTTAAGCGAATAGATAATGAATTGATAAg +caataatttaaaaatattactgTTTTTATAAGCTTTGTAACTCATGTGATATTCACGTGAGTTACTGTTTTTATAAGCTT +TGTTTATGTCTGGATTGACGATGTACAGATTTACTAGTTTTTCACAAAAAATCTTGCTTGCAAAGGTTCTAAGActagag +aaaaaaagaaaggtaACGAGATTTTGTTTGTGTACAAAAAAACATTGTACACACACTAGGCGCATATTTTATGTaacaaa +ggaaaaaatTCCGATGTAGGGCGAAAAGTGTACACAAAGAGTTTTTTCATGGGAAATGCCGATGTTTTATATGCTGATAC +CTTCttagaaaagagaaagaaattctGTAAATGCATTTACATActtaagaaaaaagataaaggAAAgttaattgataaat +tcatgaacattaataaaatcagAAATATCCTTGGGAGAAGAGAGCAATGTTAGATAATCAAATTTTAGAGGAGTTGAATC +TTGCTGACTTTGCAGAATCTTACTCGGTTCATCTTCGGTATCAATGTTAATAGTCATTCCTAGTTTGAGAAAGATAATGT +ATATTcttatacatatatatattatatcttcCCTGTCCTCTTATCTTTCTTGTGAGATATAATTTTAGGATACCTTGCCG +TTAAGTAATTCATGTACCTTAATAGGCTTTGCGCAAATATATCTTTGATATAACCGCTTTTTGCAAGCCTAAGTTTACTG +CACCTGATATGACAATCATAGCCCGAAAATCTTACTTAATGGATCTTCAGGTAACCTTGAGTCAGTTTTACAGCATATTA +GCTCAACACTCGactttttataaatattataacaACCTAAAAAGAGAACACATTCAAGTCCAATTGTTCTTGTAATTCTG +ACGGGATTGTCGAGGCAGGATATACCGAAGGTATATCctttgcaaaataaagatcatcttaattaattaagatcacttaa +atattgcatgactatacataatacaagtttactggcaataaacaatatgtatataagaagctcacatttccctaagtatt +cttagattgtatctgaagaagaaatagaaaaatacatagaaaaatatatagaaatatacagataaaaacctatctctctt +aatactatgagtaataatattagtcaaGAGAGAAACAATGACTCAGAGTTGAACCAAAGAAACTTGGCTCAACAGGGATC +ACTCTATTTGAAtagaaattaaaagaaaggCTCAAGTCTTCGGAGAATGGATTTACTAAGAAGTTCAACAAACCTTTAAA +CTGCTTGGTACTCTAAACTGATTTAAACGAGTAGACACCATTAATAtgtattgatgaaaaaaaaattccgCTTCCACTAG +AGCAGGCTACCGTAACTAAATCATCGGCAGTACTATATGTCTGTTGCTTCAGTAATGCTCTGTAGGACTGACTCATTATT +ACTTGGAAGATTGACTAGTTAAAACAATCAAACTTACCAAAGAAACTGACTATCAATACTGTCAAGCCTAGTATGCGTTT +TCCTGttgaaagaaatagaaataacaatCAAACTTACCAAAGAAATTCAGAGTCatgtgaaaaatacaaatagaAATGCG +ACAACTCGAAAGAAAATCGAGAAGGAAAGATTGGTATATAATCTAATCAATTTTCATCGTGCTCTGACATGACAGGAAAA +GAAGCTAAGGAGGATATGAATATATACAACAAGAAcaagtatatatatattatctctctcaaatGAAGAATGATTATAAA +TAGTGATTCAGAAATTACAAGAATCTTTGCAAAATTAGCAAGGTCGTTCGTCTTAACAATcccttcttcaaaaatattat +taatctaGCGTTAAAAGTACCTTTCATATCAATTGAAGATCTATCCTTATATGTAAAAATGCATTTTAACGCCAAATCTA +AATTATCCCTCTACATGTAAAACTAGATAGTCTTCCATCTTTGTGAAAAACACCGTTTAAAATGGATTCAAATTGCTtta +aattatgaaaaaaaaagctatATAAACTTTAGAAGATTCAATGAGAGTATTATCcattatcaattattaCATCTGATATA +TGCTTGCTATCAAGGATGTGGGTTCCAtgattaatatattttctaaacTTTCTTTTCCATCAAGTTAGCTAGTGGTCATT +TCACAGATCATTATAACTTTTAGAAGGTTCAGGCGCATCATCAGCTCTTAACTATGATCGTAGGTTACTCCTAATTTTca +gaataagaaaaatatctCACGAGGAAACATATTACTTACTTCCGAAAATATACATTTTTGGTCAGTTTCTTAGGGTCTCC +TCAccaattcaatttttttgtccAGACGTGGATACGCTGCTTAATATGTAACGTTATGTGCCTAGACCTAACCAGCTGTT +GCTGCTACTAGCTGTCTGTACTCATTTCACAAGAGCGCGACATAATTGAGcttcaaataaagaagataatgaCCAAGCAT +ATACCATCACGTGATTCATAAACATTCTTGTTATTTTACTAAAGAagtttgattattaatacaGTTTCTGTATTATTGAT +ACAATAAACtgtaatattaatataattgGTGTATTATTAAGGTAGGGAAGATATAGAAATAAGATAAATATGGACATTAA +CCCCAATAcggaagaaagaaagaagaagaaaaaggagGAGATAGACAgaaatagatatatatattaatatacaTTTTGat +ctctctcaaattaAGAATGGCTATTAACATTAATAGTGATTCAGACAATGAGGATGAATCTCTGCAAAGCCAGCAAGAGA +TTACGCCTCAACACGAGCCAATGTTTTTGTAGCCGAGCCCTGCCAGGGAAAAATCCTATTATTGCTATATGCTGAAATTA +GGTCAAttgttcatttttttttatttctaaatttgCTTTTTATATAGAAGGTTCGAGTGCAAATATTGTGATTGTTTAAC +AGAAAGAGCTAGCACATATTAAGGAATGATAGTTgtttaattaattattaatcatGTACTTATAATATCACGTGATAAGA +TATGTGCTTGAACAGAAAGAGCTTTGCGCGCGACGCACCATTTGTGGACAACTCCCCCATAGCTACAAATTTCACTCTGG +GCTGGAACTTCTAGCGTTTTGCTATATCCCTGTTTAGCGCAAACAAGAACCTAAGAGGTTTAATTGCAGTATAAATCTTA +TAGTTCAGGCAGATAACATTGCATGTTGGGCAGCGTTTTCACGCCTagacgaaaaaaaaaattatcatgaACAAAACAAG +TGTGTTTTTGAAGATAGGTTTTCTATATGCATCCCTAGGAAGTATTTTTTCATGCTTGCAAATAGGGTTAAGTCTACTCT +CATAATTAATATCTGAGAATATTactgaaaatttaaaatgtGTCAAATAATGAGCAAACTGCCTTCAGAGAAGAaacttt +aaaatttatattaaataatataagaaTATATGAAAACTCCATAGAAATAGTATAGAAACTGAAGAAATCTGATCTTTGCT +AAAAATGCGTTCTGTGTAAGCAATAGCGAGATTATGAAAACATCCTAAGAATAtataacaatatgaaaaatataataata +gcattATAAAATTAGAGCATAacgaacatataatatgcaCTACGACGCGTATATATGCAGGTATAATCCCAATAGGAGGA +AGACGAATATTACATTTCACGACGAAAAAGTTAGAATAtaacaagtgaaaaatccaataaaaataatacgaataatatca +agaagatataagaaaaagaaggaCAACTACATTACTTGATATTTACTTAATACCaaataaagagaataaCTACTAgtcta +tttataaaatccaAGAACGTCCCAAAAAGAGAGCATATTccaattttataatatacgaaaccttattaaattttaatgta +atatattgaacaaatattgaataaatattaaataatgaatataCAACATTTGCAACATTAATACCTATATTACTATTTCA +TGAATTTACACTTATAGTATCCAAATAAAGGAATAATCCGAAAAATTACTCCTAGCAAGAGAGcataaattttataaaag +aAACGATTTTAATGTTATATTGAACATTATAGGCAATAAAtgattaaataatgatgagaAGCGATTCTGACAACATTTTG +CAACTTCaaatccaatttttctaatCATTCTAATCGGATTACTCAATTAGAAGTGGAAATTGAATCTATGGAAACTGAGT +TCAATAAGAAATTCGACAGAATCTTAAATTGTTTATATgctctaaatttatctaataaGGATGATGTCGAGTCAGGTGAT +TACGGTTCGACTCCAAGTCTAtaacttcattatttatattgtCGTTactcatttttttgatttgagagagataatTATGT +CttcttattaatatatGTATCTACTTATTTCTCCATGCTCTCTTCTGAATctattctcttttcttcataTGGTATATTAT +ACTCCAATATATATTCCTCGATATTGTACTATCCATAAGATTATTCTGGTGAGTTATcgatttatttatatattccCAAA +TAAGGTATTTTCCCGCAGGACAAAATATTCCAACAAATTTGAAGGTATCAGATAATTATGTCTTCGCAGGACTATATTCC +AACATGCTCTATTTGAATctattctcttttcttcataTGGTATATTATACTCCAATATTGCTATTCAACAGATATACTGT +ACTTATTGATAAGACTAAGATTCTGGCGTTTCTATTAGAGCAGATATTCCTAACCAAATCACCAGCAACTCCTACTTATT +TATCATAAGAACTTCATGAAACAACGATTATCACGATGAATTAAGATTTGATTATAAGATAACTTGGGAAATAGCACACG +ACTGTCTAAATCATTTTAGAATTTctcaaaaaataagaaagaacgaaataataataaaataaagagaaTATATCTCTAGA +ATTAGATCCTGAAGCATGTGTCATaacatattctttttctaaacaatataaaatgcagaaatttttacaacaaaaaaaaa +actggaAAAGATAAGAGGATATAGTAAACAATATATTCgtgaacaagaaaataaaaaagaattcgtacataaaaaaacat +gataaaaaatatagatgATTCTAGAATTTCTCAAAAAATAAGCAAAGAAcgaaataataataaaataaagagaaTATATC +TCTAGAATTAGATCCTTACGAACAATGTGTCATAAATATTAGATATAAAATGCAGAAATTTTTCTGTATTGCACagaaat +ttaataaaataactAGCAATTTATAAACATCtagaaaaatttgataagaAACTTATAGAATAAAAAGTGAGTTAATTTAT +ATTACGaaccaataataaaaaatgatagaaaaatataaacattCTATTGACGgcaaattaataaaaataatatattttta +acatGAGAATTTTGACGAACTATTACATATTAAAGTAGTGAAACATTTAAATTTACCACACTAAATTATATGTAAAAGAA +CATTTGGTTAGAAATTCTCTAAGTTTATAAAACTAGAATTTTCAGATAAAAACTCATCCgaaaattcttctttagaTCAA +TATTAATTGTTGCCTTAAAAATCTTAACAGATTAGCAATAATATCCCCAAAATTAATTACAATCCGCTACAATTAGATTG +AAAagtcaatattttgatttttctcttATACCTTTTTGCAATATATCTAAGTATAAAATACAAGAGCatattcaaaaatc +caACGAATCGTAAATTCTTAAATATCGCAttcatataaaaaaatttaaatataattttagAAGCTAAactctattttttt +ttgtagtgaataatttttttatccTTCCATTGACgttatctttgaaattttattctGTCGCACATCCCATGAAGGCAGCT +AGCTGCTGGTCCCACATCGGCAACCTGAAACTTAGGCAATTCCCAGGCAGATGAGAAGAGCTTCCTAGCGtgcatgaaaa +aaaaaatcaatatatgAGAAAACGCTAAAAagatatatcaaaaaagcTTATTTCTGAAAATGATTTTATATGTGAATCCC +taaaaacaattttttatgCGTGCAATTTACTTAAATCCATTGCCTATAGTTAAGGGATGCATTTGtgaaaaatccaataa +aACTCGGAAAAAACTTTAAATTCACATTAAATAGGCATGCATTAGAACACATATACTAaccaaaaaatttaaacataatt +aaaatttaGAAGCTAAactctatttttttttgtagtGAATATTTGTGAAAtattaatcttttttatcCTTCACATTAACC +AGGCAAACTAAAAGCTTATGACAATAGAACACATATACTAACCAAGATATAGACTgcaatgaaatatttatgcTATGAGT +TGACAGGTAAAACTATAtcttttgatgatgaaatattcgaattaaaattaaatgaaggcaattttgataatgactttgaa +aatatgaaaaatgaatatttagaACATATAGATAAccatatttatttaaataatattttatgcATAAAATCTAGtaagga +aaaaataaatcttaGAAAAGATTTAGAAGACGAAATATAACTgcaatattttaattactATAAAGAAAcctatattttta +ttagaaaatcaaaatatgaTACAGAATATAGACAAATAGAGATAACTAACTTTGTCGGCATGCCAGATTGTTGTATGAAT +CAATAGATACAGGTAGTTCAATAGAATACTATCATATCTTATTCAGTAATAAGTGgctaataaaaaatataaaattcaaa +aataaatataaaataataaaaagtttagagaaaataaaagcttgacagaaaataattatgTTCTAtgtgaagaagaatta +ccatttttgatatttggTTAGGAACATCTGCTCTACTAGAAAAactattcttattttcgtcaataataacagtatcatcc +ttattagataaatttagagcATATAAACAATTTAAGATTCTGTCGAATTTCTTATTGAACTCATTTCCATAGATTCAATT +TCCACTTCTAATTGAGTAATCCGATTAGAATGattagaaaaattggatttgaagaattcaaaCTATCGTTGTCAGAATCG +CTTCTCATCTTGGCCTTGCTAGGAGTCATTTTTCGGATTGCCTATAATCTAATTAATGGATACTATAAGTTCTTTTGTAA +TGATTCATGAAATAGTAATATAGGTATTAATGTTGCAAATGTTGtatattcattatttaatatttattcaatatttgttc +aatatattacattaaaatttaataaggtttcgtatattataaaattggAATATGCTCTCTTTTTGGGACGTTCTtggatt +ttataaatagacTAGTAGttattctctttatttGGTATTAAGTAAATATCAAGTAATGTAGTTGtccttctttttcttat +atcttcttgatattattcgtattatttttattgaatttttcacttgttaTATTCTAACTAATTCGTCGTGAAATGTAATA +TTCGTCTTCCTCCTATTGGGATTATACCTGCATATATACGCGTCGTAGTGCATAACTTATATGTTCGTTATGCTCTAATT +TTATaatgctattattatatttttcatattgttatttcttaggataTGGCGATTATATCTTGTCAGTTTTCATAATCTCG +CTATTGCTTACACAGAACGCATTTTTAGCAAAGATCAGATTTCTTCAGTTTCTATACTATTTCTTGGAGTTTTCATATAt +tcttatattatttaatataaattttaaagttTCTTCTCTGAAGGCAGTTTGCTCATTATTTGACacattttaaattttca +gtAATATTCTCAGATATTAATTATGAGAGTAGACTTAACCCTATTTGCAAGCttgaattttataaatacttCCTAGGGAT +GCATATAGAAAACCTATCTTCAAAAACACACTTGTTTTGTtcatgataatttttttttcgtctAGGCGTGAAAACGCTGC +CCAACATGCAATGTTATCTGCCTGAACTATAAGATTTATACTGCAATTAAACCTCTTAGGTTCTTGTTTGCGCTAAACAG +GGATATAGCAAAACGCTAGAAGTTCCAGCCCAGAGTGAAATTTGTAGCTATGGGGGAGTTGTCCACAAATTGGTGCGTTC +GCGGGGCAAACCTCAAGCACATATCAATCGTGATATTATAAGTACatgattaataattaattaaacAACTATACTATTCC +TTAATATGTGCTAGCTCTTTCTGTTAAACAATCACAATATTTGCACTCGAACCTTCTATATAAAAAGcaaatttagaaat +aaaaaaaatatgaacaATTGAATTAATTTACAGATATACAATAATAGGAAGCCCTGGCTTGGCTCGGCTACAAAAACATT +GGCTCGTGTTGAGGCGTAAATCTTCATGGCTTTGCAGAGATTCATCCTCATTGTCTGAATCACTATTAATGTTAATAGTC +AGTCCTAAAATGGTTTGCTGGcttaacaatttttttattaaagaaatgaatGTTTATTATGGAAAACCTATGAAATTTCA +TATACTTAAAAATTGGAATCATTGAAGATCAGTTGTTCACTTTTGACAACTTATTAGCACATATTTAGTTTAAATCTGCT +GACTAACTTTTTGGAATTCAGGGCTTTTGCAATATTTTTggttttaaaaaatttaacGGTAATCCTTAATTGCTGAGCCA +AAATACACTGTATGACTGACTCAAGGTTACCCAGGGATTGATTACGTAAGATTGTCGGAATGATATTGCCAGATCACGCA +AAACAAATTAAGCCTTGTAAAAATTAATAGGCTAAAACAAAGATATAGATTTACATAACGCCTTGCTGGCTTTGCATAGT +TATGAAAGTgtatgaaattattaacagTAATAGGAAACAGGTAAAATTACTAGTGAGAAcaacaatataaatatgaatCC +AGATTAAAAATCCTATGAAATATATCATATAAGAAAATTGGAAGGCAACGAAGATATTAGTATACAACTTATTAAcatta +tatttaaatctGCTCtaatttttggaattcaGTTATGCAATATTTTTAGTGTTTCTAAAAAAGAGTAACGGTAATCCTTA +ATTGCTGAGCCAAAATACACTTATGACCTCAAGGTTATGtctttttattgattaCGTAAGAGGTTGTCCGAATGATATTG +CCAGATGTGCAAAACAAATTAAGCCATAAAAGAGGCTAAACCAAAGATATAGATTTATATAACGCctttattttcttatt +gtACCATAAATTAAACCTTAACAGTAATAGGAAACATTTAAAATTTAGTTTAGTGTCcgataatatatttaaaattccAA +TagctttgaattttcatattcttcCTCAGAAAGACAGGCAACGGATTATAGtatttctaaatttataaGATATTTTCTCG +AATCAATGTTATCTTTAAATAccttttcttgaaaaaagtaaGAATTTCGCAAAGCCAAAAATCATTTTACCTCAACAGTT +TGACTTTTTAAACTTCAATGAGACATTCCGAGCTTCAATTTTGATTCACCAAATTTCGTCCAAAAAAGCATTTCTAGGGT +TTATAAAAACCTTTTCAAatgtattttcttttgtacCATAATTAAACCTTTTAGCATGGAGAACTTTAGTCTAACATGCA +GATATGCCATCTTCATATGACCAaagtttgaattttcatattCTGGCACTCATGCAAAGACAATAACGGattttatttct +aaattttcaacaataaaaCGTTAATGTATTCTTATAAAATACCTTTTCTTGGTTAAAgttattcttctttatcgAATCAT +TTTACACATCTTTGCGACTTTAGCATATTGCTGGAGCATAGGATCAGCTCTTACCTATGCAATTTTGATATACCACATTA +TTATAACTAATAACgcattatttattcacaAACTATAAGCCAAGTTCTTTTCAAATGTATATTATAGTactctttatttt +tagcaTTACACGCTGTTAATTTGGGAGAGAGCATGGAGACCTAAGTTATGCTGTTGCAGGTGCTGATTTCGCAATTATGC +AGCCGATAACCTTTATATAGTATAAATCCAagtaaacaataaaataaatttattagatCGGActattaaaattgaagcCC +GAGCTTTCCTATTTGTCGAAGTATTAAGTCGTTCTATTATAAATGCTGGCTCAGCAAAGGATGTATCGGAGAATCTGCAA +TTCTTATATACACATTATTATCGGCTAATAACgcaaatttttattattgaactTGAGCCAAGTATCTTTCAACTTAACTT +TGAGTTATAGTTGTTTGATTAGTGATATTAATCTAAATTATGGGATGAGAGCTTAGAATAAATATGctttttgatatata +aTTATCTTCTACAACTGTATTATGTATATTCCAATCGTTAACATATAAATGTAAAAACTCTTATGGAAATGCGGACTACT +ATATATTGCGTTCTCGATATATTTCACTGCGGCATTTACTGATTAGTAAAGTGTGCTACCATTATGCGAATAACTGGTCA +TGATTTTATGATATGTCAAGCTAATATCTGGTCCACGTGATCTGACAATATCTTGTGCTCCGACATTAAGTATAACTGTT +CTTGTGAGATATAATCTGTAAGAATATCCTATGTAAATGCgagcttcttatataccaatttttattaacaGCAACTTACT +TATTAACAGTTTTTCGTTATATAATGTTCTCTTATATATGGCTGAGCTATCTTTGATTTATATCCTGTTCGGCAATTTGA +AAGAGTGAAAAGTTCTATAAGGATTATGCGGATGGATGGATAACTTCGCATGTTTTATGATATGCAAGAATATCTTGAGA +ACTGCAAGCTTCTTAATGCTACTATTTAATAATCTTGAGCCGATAGTATGCTTAAGACTGGCCCAAGATTACATGATCCT +TTGATTAAGTATTATTGTCGGGACGATAATATTGTCGTATCACGTCAAAAAAAGCTAATGCCAAAATAGATATTAGAGGT +TAATCATACAATATCTATGACCATATATAATGGTACAGTTTCTAAATAGTGACTATAAGTAATGTGAAAATTGGCCAGTG +AGAACAAGAATGTAAATATCCACATTTTCGAGTCATCTTAAAATTATATCACACAAGaaagatattaatatatatatgta +taagAATATAGGTTATCTTTCTCAAATTAAGGATGATCCTTATACATTAATAGTGATTCTGAGAACGAGGAAAAATCTCT +GAAAAGTCAAAAAGCCTTTACGCCTTGACAAATGATTTATGTATTTCTCTTATAACTAATATAGATTTTGTGTACCTAAT +TACCTTTAATGTAGCCTCTTTACAAGAAAGTACATATCAAAGATGGATCAATTACATATGTCTCTTGCTTTCCCTGAATT +AgaatattcttattattttcagaacCACTTTAATACTCATACCTAAGATAGATAAAATTTCTCGTTTATTCTTACATTTC +CTGCatcttccttttcttctctttaACGTACAGATTTAAGAAGTGTTAGTTTAGactttctaataatttggaTTAGGATC +ATCTGCTCTAGTACAAATTGTCTTGCTTTCATCAATTcaataacagtatcatattttttagataaatttagagcattcaa +attttaagaTTTTCGAATTTCTCGTTAAATCCAATTTCCATAGCTTTAAATTCAGATTTTTACAGATTTAAACTCGGTCT +TCATGGTATTtattgttcttcttctattattgaatgattCGATTCAGAGTGACTTGACCGATTGGAATTGAACGAATTCA +AACTATCATTGTCCGAGTAGGCTTTGACGAGtagtttattctctttaaatgGTATGTATTATCTTGACTTTGGTAGGAGT +CATTTTCGGATTATTGTTAATCCTGATTTTATGATActgtaattttttgtaatcGTGAAACGTAATATTCACCTGCTTAA +TATCTAATGTGGTCTTATTTATTGCATATTTACACGCGTCGTCATGCAATTATATGTTCGTATGTTATcattttatacta +ttactattatattcttcatatttttatttattaggATATGGctattatatcttgacaTTATTCATTTCAGAACCTGATGA +AAATGGACTATATTATATACTAATCTGTCTTCCTTCATGCTCTTGTTGATTATTCAAGCAAATTTGGGAgcaattatatt +tttcaccTTTATActctaattttttcgtcGTGAAACGTATATTCACCTGCCTACTGGTCTTATACTTGCATATACACGCG +TCGTcatgcatattatatgttcgtttgttatcattttatactattactattatacagattcatatttaaatttattgata +tgGCTATTAATCTTGACATTAATCATTTCAGAACCTGATGTGGTGGATTACTATTAATACTAATCTAAAAGAATAACAGT +TCATTCATGCTTCTTGATGAATTAAGCAAATTTGGGAGCAATTACAATTTCTATTTAATAATGGCCCTGCTTTTCCAAAT +TACTTATATCCCAATGTACAGATGCTACTGAgcttaaaaaaattgatacaACAATTTTCATGTAATATAATAGCTGTGGT +GTATTACTGGctaaatttgataaaagaaaaatttcatatGAGATGATCTTCTTGCaaattaaatcaaatttcATGAAAAC +AATGTAACAATAAATAGTTGTTAAATAGATTCGGCTTATATAATGAAACTTGgtttaataaaagaattaaagagAACACA +TTATTTActatttattgatatttgaataaaacaaaaatttcatcCATGAATAATGCATGATTAAGCaaattaaatcaaat +ttcATGATTAACTAAACggaaataatttcaattccGATAATCGGCTTATGGACATGATAACTGGTTTAATAACAACTCTG +AAAATATAACATAGTTTAAATCTATTGAATTCATATACAGCATGACTCTGtcattattgatgaaagtaaaaatattgtCT +ATACTGGGGCATATGATCCGAGCCAAATCATCCGACACcccattttttcttcacaGTATATACTATGTGCCCTGAAATTA +TCTGGCTCATGAAATtctataaaaaaaaacgcACTTCGTTGaacttatttattttttggatACGTATACTTTTGACAAAC +GATTaagtaaataatttataaaaaattcagaaaaagCTAATTTGTGCTGAATAATGTTTATTGTTATGAAACGTATGGCA +AGATATGACGTCTTATTTGCCAAGAAGGgcaattttcaacatgtaTCAATTTCCAACAGCAATATATTGATAGTGCTATC +GAATTTTATAATGCTGTTAATAACTTATCCGAAGCTGTGTTAAAAGGTTgtgaaaaaattggaaattgTGATATAGAAAA +GATTAATGCTTGAAGAAAGAAGGCACTGAAATAAGTGGGGGGaaagatataaaaaaaaaaactcaTAAAACATGTCATTT +GTGCAAGAAACTCTGCTAGGTAAAACATTGGATGGCAAGAATAAAAAGCTACAATGGTGGTGTACTACTCTTCTTAGGAA +TATTTTTGAGATTAAGAAGACAAGATACTGTAGATTTTATCTAAATAATGAACCTACTAACATTTAATCCTCGTAGAAAC +TATCTTTACagaaatatacaaaaatatcTAAATTGATCATCAATTCACTGAGTCATGCACTTGTCATCAAAATGAAGGAG +CTGCAAATATATTTCCAATCCctaattgatatttttatgatGTTGGCACCAATAGTCGGATGCGGCATATGAGTACACTG +GCTTCATCCTGGATATTGGTATGCATTGACGGAATTCTAGATAAGAATATCATTTGCTGTTTCTGAGATTAAACTGCttc +aaaaagttgaaaataagTTTGATTTGTATTTAAGTGTTCTTCTGCTGATACCATATTGTTCACTCTAGCGTTTATTAGTA +GCATAAAACATCCCatagattaaataaaaaaatggcGACTCCCCTTGATACAGTACCCACTCTGAAAAAGGATACAAAAG +AATCAAAGCTTGACAAATCACCAAATATAGGGCAAAAACTTGAGACTAAGCCGAATAAGCTTGACAACATTCAAAATGAT +GTTACAACAGCTGAGCAATCCTATTATTTTGGCGATGATATTCATAATGATGAGAACCATACAAATACATACATCGACCA +TAATGACTACGAACTAACAAGACAAATATCGCGAAGCGTAGCCTCAACATTGATTGGTGGTTCCTCTTTTAATGACGTAT +ATGACAATGACCAGGGAGTagtagaagaagaggaagaaaatggaaatgaaaatgaaaatgaaaacaaagaagaaagagag +AAACAAATCCTGAAAGGCGATGCAAGAGAAGCAGTGGCAGtacagcagcagcaacagaAAGCAGTAAATGTGGGAACTAT +TgctaaaattaaaaatcaGGGTGAGGAAGCCGCGATAATTGCAGGTAACAAGTTAAGAAATATCATTGATTCTATGGAAc +tagaaaagaagaaattcaCGGCCACTCGACCATCGAGACACAACAGTCTTATGCTGGTAGAAAGCTCACTTAATTATATA +AAACAGAAATCATCgtttatttcttttgggaaaaaaaatactacACATAGCAATACTACTAGTAGCAGAAGCAGCAGTAG +AAGTAGCAGtaatagtagtaataatggAAACACCAATATTTGCTCCTCGCCTAAGAATAAGACAGCTGCTGAGAATCGAC +GGGTATTTTCATTAACTTTACCATTTTCAGATACACTTACAGACATTCTACCATCAAGGATTAGCATGCCAAAATTgaga +ttttttcaaaacgATGACAGCAGTTATCCACTTCCGGTGCCCGAGAACATTACAGACAAGCAGCTTCGACAGAAATTGAA +GCGACAGGATTCTATGAGTACACTTGAGGCGGCTTATGCTTATAGGGAGCTCAAGGGAGCAGACAACTCGAGACTTCGTG +CGGTGAAAAAGGCACTTACACCGGCGTTCATTAGCGAAAAAACTGGAGAAAAATCATATGAATATTACACGGAAACGGTA +TTTGAAAAGCTAGAGGGCGACATAGTGGTGCTAGGAGGCTATCGGGGGTCTGTTTTAAGAGACACAGAAACAGGGCGAAT +GCTTTGGATCCCGATGAAGGTGGGGCTCAACTTGAGAAAGGTGGATTTGGTGGTGCCTCCGGATGACCAAGCTGAGTATG +ACATGCCTAAAAAGATATACCCTGATGGGATGTTGACCAATATAGGGCCTATAGATATCTCGAAGAAACTCATCAAGAAG +ATGGCGGCCGTGCCCGGGGTACGAGTACATGAATTTGGGTATGATTGGAGATTGTCTTGTGATATCAATTCAGCAAAGCT +TGAGGCGTTCCTTAAAGAGCTACCatgcaacaacaacaacaacaataataataataataataataataataataataaca +gtaataataatacgAACGGTGAAAAGggcaaaaaaaacaaaggaGCGTTGGTTGTGGCACATTCAATGGGGGGATTAATT +GCACATCATGCAATGAACAAAGATCCAACGCTTTTCAATGGTATTTTATATGTGGGGACCCCTAGTGAATGTCTTAACAT +TCTTGGTCCTATAAGATTCGGGGACTCTGTATTACTCAGTAAGAATGTTTTGACAGCTGAAGCGAATTTCCTGATGCGTT +CAAGCTTTGTGTTCTTGCCTAGACATGGAAatgttttctttgataCAAACACGGAAAAATTCATACATATGGATTTGTTC +AACCCTGATACTTGGGTCAAATATAACTTGTCTCCATTAGTATCTAACTCACACAAAGGCATGCAATCTTATGAATACCT +TTCGCGTACATTACAAAGAACTAAAAAATTTCTCGACGAGTTAAGTTACAATCCagaattgaaagataaGTATCCACCAT +TAGCTTTAGTTTACGGAAATACTGTCCCTTCAGTGCGAGGTGTGTTGGTCGATGGTGAGAAGGACATTAAAGATGGTCAA +TACGATAATTACTTTTATGGACCTGGTGATGGCGTTATTCATTCAAAATGGTTGATGCCTGAAAATTATGAAGGTTTTAA +AACTGTAGCTAAAGTTCCTAGTGATTGTGGTCATGTTAGTTTAATGACTGATTTTGAAGCTGTTGGAAAAGCTTTGGATG +CTATTATTCACCATCCAAAAGCCTTGGATGCtgttcatcatcattaaataaatacatgtatatatataaaaaaaaaatag +atatatatatatctataacagtatagatatatatatatctattttttttttttataatttttttataatttttttatagt +cttttctatcatttttttagcTTTGAGTTCAAATTgtaatgattatttttggGAAATAATTAGCACGCAGGACAATAAAG +TTTATGCGCAAAAAATGTGTACAGccaaaaaaattctaatttatttaagtattatttattatatagtTGCTTTATGCGTC +ATAGTAGATGTCAAATTAGgattaaattttttgctTTCCCACTTTACTGGAGAGTATAGAAAACTTTGAGAACATCGGCT +GTTTAAgatctaatttttttgattttctagGTTTTCCATATTGAGTTTTTTTTGAGTTTTTTAATATGTCAAATGGTAGT +GCGACCAGTTTTGTATCACAATGAGCATTAATCTTCAATGTCGATTCTAATATTGTGTTCTCCTTTCCTAATAGTGAGCT +TGCACATAAATCAAGATACTCTTTTCTagatttcattaatatgGTTTGAGGTTCAATTTGCTCTGCAAGGTATGATTTCT +CAGGAACTTCATAGACAAAGGTACCTTTCATATTTAAGTGCTTCTTATTTATggttctttttgttttgaatcttaaaaaa +tcattatcaaatatctGCTGGTGAAGATTATTGTCGAATATCGCAagataatcaaaataacTCACAAAGTTCTGACCTGA +GTTTCTCCAGATATCTGAAACTATCTTTGATACCGTTTGGCTATTTATCGCTGTCTCTTTGTATCTTAATaaacaattaa +taaaactTCTTGCTATCATGAATTTATTCCTAGGTCTTGTTggttcttcttttctattattatttttattactcTTTTTC +AGTATTACGGTAGATACAATAACATCGCAATTAACTGCAATAACATTGTATGTTTCATAACTTGTATGTTTTTTGGTGGT +TCTATCCATATAAGGAAATCCAGTAACAGCTATATTGttatctttatttgtatttaCTTTAGGTAATTGGAAAGAtagta +ttttctttgacaTATTAGGTATTGGttaaattttaatgaaCAGTTCTAATTTGTGATATAGTATATAGTATTGGAATAAT +TTAGTGATttaaaatgataaagaaaatttttgaaataatttatttttgaaggaaaagaaggttattataaataaaaaaaa +aatatatatatgcaagaaataaaaaaaataaaaaatatgaaaaatatggtATCAAATGTGATTCATAAAAAACGATTGAT +CccaatttgaatttttatcACGTGTCTTTGCACATCTTTCTCATAAATCTTGGTTTTGgttgttttttcttgacTGATCA +AATATATGGTATTTAACTATAGTTTGCTTTTTTGCTTCCTAACttcatataaatattattgtttccTCTTAAGTTTATTT +CTAATTATcgaaaaaagtaaaaaataacTCATAAATATACTGCAACATGAgtgataatgaaaagattGCTATTACGGTGG +GAGAAATGGATTCAAAGTGTGTGATAAGGATTCAgtttataataaaacagAAAGGGTCGCCAAGTTAAGAGACACGGAGC +AATGCATCATACGTACAACTGGTAGAACAAGTGCTGACATCTCCATTTCTGATTTTACAAAAGATACAAATAGCTATGTC +CAGTATTATCATCTTACAGAGTCTGAACATATTCCAGAAGAGTTGAAATTAAGTTATAAGCCATTGATTGAACGCTATCA +GACTTATCTCAACACAATAATCAAGGTATGCCATACTTTCTTTCtcttatattattttatgaGACATTTACTAATTAATT +TAGTTATCGGATGCAGCAATAAAGAGTGGATTCAAAGTGTGTGATAAGGATTCAgtttataataaaacagAAAGGATGTT +GATCGTCGACATTTTCTTATCACAATCCACTCTCAAATCCAGCAAATATACAGATAAGTTAAGAAAGTATCTTGAAGATA +GAGGTGTAGATATAGTTGTTTTAATGCATGACCAGCTGCAAACGTCTAATAATTCGAGATACTCTAGGAAAACTAATGAA +TATCTAGAACTTCTTTTCGATACAAATCCTAGACCTAATTCTCGTGAAAGATTATTCATAGCGAAGCAATGTGGATTAAG +TGCCAAACAAGTCAGAATATGGGTGagtattttattattattattattattattattattattcttcttcttcttcttct +tgttgttgttgttttatacaaatatatatatatatatatatatatatatattatttgtgTGGGACGGGGGTGGAGGAGGC +AGCAGCAACCtgaaatttatatttttatttggcAAGCAATATCTAGAACTAACAAGTATTTTTATAGTTTACCAACAGAC +GTGCTAGGGTCAAAAAGACACAAAAGCcatagaataataatttattattacgtaaatatatatatataaagagaaagaga +gagaataaataataaactttaatctttgtttttcttgtttttcttgttttttttttttttttttttgttttttttaattt +ttcttttctaattaATCGTATGttgtaatatttctttctttaaataaattttttattgagTGATATACTGGAGGAGTAAG +AGtgtaattttaatttaattaatatatctaatattcttcatcattatcgctatcatcatcatcatcttgataactatatt +ttctaatatcaCCTAATCTCTTTCTAGCAGCACCTAAGGCATTCTCCAACTTGATGATTTCTGCTTGttgtttcatttct +tcttttctattatcAATGATACTCAACTTGGagaaatcaatatcatcattaacTAATTGTGTagatttattaatcaattc +tTGCACTTGGCTTACTAAGCTCTTGCAAGCAATAGACACCTTCTTACTTGCATCTTCCAAATTGTCTTGAGTCTTGGAGA +ACTGCGTAGCTTTAACTCTTGCAGCTGCCACTAGCTGAGCAGTAGAATTTCGTTGTCAACTTCATTAGATGCCACAATCA +ATCCTTCTGTGTGTATTCTTACCACTCAAACTTACCATCCATCTTGTTGAGTTGCTGAATTAATGCCACCGGTAGCCGAT +GCAACAGCTTTAGCAGCAGAGATTCAATCCTTCTGTCCATCTATTATTCTTTCTGTAGACTCCTTGGGCTTTGTACTTGA +ACCTTTACCATTTGCAACAATTCTTGTTGAGTATCGATGGCTGCAGAGATTAACATCGAAATAGCTCGTGGTGATAGCAA +TGGAGTGCTGCCAAAATAGCCTCAATTGACTTCTAAGTTTCATGGTGATTTCCTGTAACATTCAATGGTTACAACGTCGT +CACTTTGTGTTCTTGCTTGATAACCTCCGCTAAGCGTTCATTTCGTTGTCCATCAAGCTTTCTAACTCCtctttgtttgt +ttttCGTCTCAATGAACTtcttgttgctgttgctgctgctacTGCCAATAGCCGCCGCCGCCGCAAGCCACCGCTCGTAG +ATGCAATAGTCTTCAATGTAGCCTTAACTTCAACTCAACATCTAATCAAGTCAATTTCAACGACAATTCTCAGCAATCAC +CGCTTTGTCTGACTTAAAGTTCTTCGCTCAAGCTTTCTAAATTCTTAgcaatttttccaaaaattcaatgCATGTCTGAC +CAACCAATCTTGCATGAAGAATCAGCGAGTTTTCCAAATTCTCTGTCTTGCAAAACTTAATGATGCCTTTAACATTCAAC +TCAACATCTGTAATACACAGAGTGAATTCTGAAATAGTAGAAATGATCTCAGCATAATCACCGCTTGGTCCATCAGCGAT +AAAGTTATTGAAAGCCGTAGCAAATTCTTGACTTGTTTCGCCtgctttttcaataatggtTAGCAAGTATGATGGTGATG +AGTTTTGATTACCGCCTTGCATCGAAGAATCAAGCTCGAATAAACTATCTTCGATTCGTTTCACACCGCTTTGCAAAATG +GAATCTATAATAGCAACTAGCTTCATGATATGTTCTTGTAACACTGTATCAATTTGTTCATCGATAGCTTCTTCAAAGTT +TAACTCGGCTTCTTTTTTCAGAAAGAGTCCTCGGCTAACTGTTTTAACGCATCATCAATTGTCTGTTGTAAAATTTCGAT +TTCCTCATCCTTTTCACGCAATGTTTCCTGTAGCTCAGCATTTGACTCTGGTGACATCTCTGAGATTTGCGattctcttt +ctttcaatttggTTTCAAGTTCTTCCATTTCTTTTCGGTGTGCGTTGAAAATGGAGTTCAAATTAGACGACTGGTTTCTT +TCTGTCACATCCAACTTATCCTTCAAAGTATTAACTTCGGCTTCTAGTTTTTCAATCAAAGAGTCCTTAGACCCTCTTAA +CTTGTCAATCTCCAACCTAGCTCTGTCACGTTCTTTAATCAAATCTGCCAATTCAACATTCTTCGCCTTCAAATCTTTCT +CAATCCTGTCTCTCTTATCAATAGCCTCCTGTGCAGATGCAGCCTTTTGTTGCaactttttgaatttttgcaataaattc +aaatgtTCTTGTCTCAATTGCGAGCTACAATTTTGCTATGCTCTCATATTTGCTCTTGAACAAGTTCAATTGTTCTTGGA +CTATGATATTGTTGTAGCATTTGTTGCTGTGCATTATTAAGAGTTGCGCATTCTTTCTCTGGCTACTTTCTTGTTCATTG +TCTTGTTGAGCTTTCCAGACTTGATTGATTAAAGGGCTTGCTATTTCCTTTGGTGGCGGTGGTGGCGGACTTGCCGTTGG +ACTCTTCGCCGCCGGCGCCTTGGTAGGTGGTCTACGAATACCCGTGTTCTCATCAATGAGCAAATCCGGAGGACTGGTCG +TCAACTTTGGAATGTTGATCAAAGACGTCAAATAGTGAAGGCTGTAGGAGTTACAGTAATTCCAATGAATAACGATTCTT +ATTAAAGCTATGCAATTCGCGTTGTTaagattataataaatgcACTTGCTGAAGCTTGGGAATTCTTCTTGTTATAAGAG +TTGCGCATTTCTTTCTCTGGCtaaaatttcttgttcAGCTTCCACATTGTCTTGTTGAGCTTTCCAGAATTCAACCCCAG +TAGGTTGATTGATTAAAGGGCTTGCTATTTCCTTTGGTGGCGGTGGTGGCGGACTTGCCGTTGGACTCTTCGCCGCCGGC +GCCTTGGTAGGTGGTCTACGATAAGGTTCCGGACCCGTGTTCTCATCAATGAGCAAATCCGGAGGACTGGTCGTCAACTT +TGGAATGTTGATCAAAGACGTCAAATTCCTGTAAGGCTGTGATTAAGGAACAATAATGTTACATTTGATTAACAATGAAT +AACGATTATCTTATTAAAGCTATATAAAAACTAATTAACTaagattataataaatactGAAAGGTGGGAATTGCAACCCT +TTATATATGAGTCTGTGGGTGGCAAAAATATAGCAAAAACCACAGTCACAACTAGCAGGTCCCAACTATAGGAGAATATC +CATATGGAAGTATATGATGATGTAACAATGTAACAGGAATATTCTTGTAACAATGCCTAAGTTTCATAGTCTTTTAGAAT +CTAAGTAGTTTTTCTGGAATTCTATGAAACTTTCCGGAATATTCCTGGAGTAACAATTGGTAAAAATGATTAAGTAgaga +ataacaataatgataagtataatttgaataactGTTGAATGATTGGTAATAAGTAATAAATGATTACCAATCCGAATAAC +AATGGATTTTCACAAATACTGCATCAATTACTATGATCAAACAACATCAGTATAACAAGCGCAATAGACATTTCAACTTC +GTCTTGCCTACTGTCGACATATTATAACATGAAACAACTCCATTTTAACATGTCCTTTTAATCAAGAAGCTCAACTTAGC +TGACATCCTTGACCAAACATTAACTACTATCCCCTTTTCCAGCCTAGTGCCATCTCTTGTCAAATAACATAAGTTTagtt +tattcaatttcacttcaaaaaaaaaaaaaaaaaaatataaattatgtcgaattatagataatactatgaggcTTTTTACA +ActgattactaagaatgtgcCTTATGATTAGTAAGTTGTGTGCCGTGATGCTACTAATACAAAGCCgtttaatcattaga +ttaaataatcaaggagaaatgaaactactattatatataaagaacctaAATCCACTTTCTGCTTATATATTTCgtcattt +tattatttattttaattccttttaatcaagaatcctacttattaattattgatatacaaattgtatcatttatttattct +caacaatgttttatatttaatatattttctctattacaTATTGGTAATAAGTAAGTAAACTCCAATACACTTCTGCtcct +tcaagtgatttacttttaacaatcttattctCGAGAGCACCAAACAGTCATTCCGATTGAATCCTTTATATATTAGTTGT +GGGTAGCAAAAATAGAGCAGTCACAAGTAAAGCATAACAGGATAAGAGAATAGCCATATTGTAGTTTATAGTGATGTAAC +AATGTGCAGGAATATTCTTGTAACAATGCAAGTTTCATAGTCTTTTCGAATCTTCTAGAACTTTCTGGAATTCTATGAAA +CTTTCCGGAATATTCCTGGAGTAACAATTGGTAAGGATGATGAAGTAgagaataacaataatgataagtATAATTTGATT +AACTATTGAATGATTGGTAATAAGTAATAAATGATTACCAATCCGAATATCAACGGATTTTCACAGTTCAAGAGCAAATA +TGAGAGCATAGCAAAATTGTACTCGCAATTGAGACAAGAacatttgaatttattgcaaaaattcaaaaagttGCAACAAA +AGGCTGCATCTGCACAGGAGGCTATTGATAAGAGAGACAGGATTGAGAAAGATTTGAAGGCGAAGAATGTTGAATTGGCA +GATTTGATTAAAGAACGTGACAGAGCTAGGTTGGAGATTGACAAGTTAAGAGGGTCTAAGGACTCTTTGATTGAAAAACT +AGAAGCCGAAGTTAATACTTTGAAGGATAAGTTGGATGTGACAGAAAGAAACCAGTCGTCTAATTTGAACTCCATTTTCA +ACGCACACCGAAAAGAAATGGAAGAACTTGAAAccaaattgaaagaaagagaatCGCAAATCTCAGAGATGTCACCAGAG +TCAAATGCTGAGCTACAGGAAACATTGCGTGAAAAGGATGAGGAAATCGAAATTTTACAACAGACAATGGATGATCATTA +AGTTTTGCAGTTACCGAGTCTAATTTGGGAAAGCGAAGAAGCTATCGATGAACAAATTGATACAGTGTTACAAGAACAAT +ATCATGAAGCTAGTTGCTATTATAGTTCCAAGAATTTAGCAAAGCTTGAGCGAAACAAGAAGTCAGACAAAGTTATTGAA +GGTGTCGATGAAATCCATATTCTTCGATGCAAGATCAAAACTCATCACCATCATACTTGCTAaccattattgaaaaagca +GGCGAAACAAGTCAAGAATTTGCTACGGCTTTCAATAACTTTATCGCTGATGGACCAAGCGGTGATTATGCTGAGATCAT +TTCTACTATTTCAGAATTCACTCTGTGTATTACAGATGTTGAGTTGAATGTTAAAGGCATCATTAAGTTTTGCAAGACAG +AGAATTTGGAAAACTCGCTATTATCATCTGCAAGATTGGTTGGTGAGACATGcattgaatttttggaagATTTATTGTCT +AAGAATTTAGAAAGCTTCTGCAAGCCATCGATACTCAGACAAAGTTATTGAAGGTTCAAGTCGTTGCCCAATCcacaaag +aagaataattgACTACAGAAGGATTAATCTCTGCATCTACGAGCGGTGGCGGCGGCATCGGCGGCGGTGGCAGTAGCAGC +AGcaattcaaaagaagatgGTAATTTGAGTggtaaaataaaacagaAGAGGAGTTAGAAAGCTTGGTGGACGAACGCTGC +TGCGGAATTGATACATTACACTTGACGTCGTTGCTAACCAGCAAAAACTTACAGGACTTGTCACCTATTGACTTAGAAGT +CAATAAGGCTATTTTGGCAGCATCCATTATCACCAAAGCTATTTCGATGTTAATCTCTGCAGCCATCATCTTGAGATTGT +TGCAAATGGTAAAGGTTCAAGCTACAAGGCAGCTGCTAAGAGTTAAAGCTACGCAGTTGGACAAGACTCATTAATTTGGA +AGATGCAAGTAAGAAGGTTGTCATTGCTTGCAAGAGCTTAGTAAGCCAAACTGCGGATGAATTTGAGTCTACACAATTAG +TTACATGAGATATTGATTGTGGCATCTCCAATTGAGTATCATTCtataatagaaaagaagaaataaactACAAGCAGAAA +TCTCAAGTTGGAGAATGCCTGGTGCTGCTAAAAGAGATTAGGTGTCTATTAGCTTAGTTAAAGATTATAAGcgataatga +tgaagaatattagatatattaattaaattaaaattacaCTCTTACTCCTCCAGTaatcatcaataaaaaatttatttaaa +gaaagaaatattacaaCATACGATTAATTATTAaagtttattctctttatatattgatttacgtccaataataaattatt +attctatgGCTTTTGTGAAACAACAAGCAGAAATCACGTCAAGTTGGTAGAACTTAAAAATGCTAGAAAGAGATTAGGTG +ATATTGCTTGCCAAATATAGttaaagatgatgatgataaatttaatgatgaagaatattgatatattaattaaattaaaa +ttacaCTCACTCCTCCAGTATATCActcaataaaaaatttatttaaagaaagaaatattacaaCATACgattaaaaaaaa +caaaaaaaaaaaaaaaaaaaacaagaaaaacaagaaaaacaaagattaaagtttattatttattctctctctttctcttt +ataatatatatttacgtaataataaattattattctacTTGTTTGTGTCACTTTGATCCTAGCACGTCTGTTGGTAAACT +ATAAAAATACTTGAATTAGTTCTAGATATTGCTTgccaaaaaagaatataaatttcAGGTTGCTGCTGCCTCCTCCTAGA +GTATCTCGAATTATTAGACGTTTGCAGCTGGTCATGCATTAAAACAACTATATCTACACCATAATATTCTTAACTTATCT +ATATATTGCTGGATTTGAGAGTGGATTGTGATAAGAAAATGTCGacgataataataattaattttattataaactGAATC +CTATCACACACTTTGAATCCACTGACTTGTTTGGCATCCTAACTAAATTACATTGTAAATTCTctatgaataatatttca +cGAGAATTAGGTCTAGGATTTGTATCGATACCTTGATTATTGTGTTCAGAGATAAGTCCTAGCGTATCAATCAATTATAT +AACGTTAAGCTGGTCAACATTAAAACAACTATGTCTACACCTCTATCTGTAAGATATAATACTAACATAGCTATATTGTG +CTGGATTTGAGAGTTTgtaataatcaaaatgtCGACGATGTCAACACTTGTTTTACTGAATCCTTATCACACACTTTGAA +TCCGTGTCTCTTAACTTGGCGACCATTTCTAAATTAATAGCAATCTGTTCATTAAATCACTCAAGAGAAGTAGTATTTAT +GAGTTATTTTATTACTGTTTTCGATAATTAGAAATAAACTTAAGAGGAaacaatatattaatatgaAGTTCTGGAAGCAT +GTTCAAACTCTAAGTTAAATACTGGACATATATTTGAtcttttcaagaaaaaacagaaaaccATGATTTATGAGAAAGAT +GTGCAAAGACACGTgataaaaattcaaattggGATCAATCGTTTTTTAACTTGAATCCATTTCTACCACCgtaatcaatt +ttttattatcattgttgcagtatatttatttttattttttatttatataacCTTAGAAATTTCCTTAAGAAATacaatta +tttcaaatgaaGTTTCTTTAAGCAAAAAAGCAATCACTAAGTTAAATTCCAATATTTATACTCATATCAAGATTAGAACT +GTTCATTAACCAAGATTTAACCAATACCTAGATATGTCAAAGACACGTGATACTAAATTCAAATTACCTATCAATAAATA +GAAATCACATTTGATaccatatttttatattttttattttttttcctttatgcatatatatattttttttggataGAACC +ACCAAAAAACATACAAGTTATGAAACATATTTTATTGCAGTTTCTTGCGATGTTTTAAATGTAAATACCGTAATACTGAT +ATACTATataaaattagaagaaaagaagaaaatttaacCTAGGACCTAAATTCAtgatcaaagaaaatactaGTCTTTAA +TTACCTGTTTATTATAGAAATAAAGAGACAGCGATAAATAGCCAAACGGTATCAAAGATAGTTTTATATATCTGGAGAAC +CACTCAGGTCAGAACTTTGTGagttattttgatttcttgcAGATATTGCGATGTTATTCTTCTACCAGCAGATAtgaata +atgatttttaagattcaaaacaaaaagaagaccAATAAATTCAGAAgcaagaaattttaaattgttTATTAAGATACTAT +GAAGTTCCTGATAAATCATAAACTTGCAAAGATATTTCAGATACCTGGAAACcatattaatgaaatctACCTTTTGATTA +TCTTGCGAAGAGTCTTCACCATctatttgataatgatttcaTGTGCAAGAAGCACTTATTAGAAAGGACCTTTGTCTATT +AAGTTCGACATTGAAGATTAATGCTTTGTGATAGCAAAACTGGTCGCACTACCATATTTGACATATTAAAAAACTCAAAA +AAAACTCAATATGTGAAAACCTAGAAAGGATCaggaaaaaattagatCTTAAACAATCGATGTTCTCAAAGTTTTTAATA +CTCAGTAAAGTGGGAAAGCAAAACTTTAATCCTAATTTGACATATACTATGAAACTCATAAAGCAACTATATGGAAATAA +TacttaaataaaaagaattagaTCTTGGCTGTACACATTTTTTGCGCATAAACTTTATTGTACTGCGTGCTAATTATTTC +ccaaaaataatcattacAATTTGAACTCAAAgctaaaaaaatttagacAAAGActatgaaaaaattatactaaaataata +aaaaaaaaaatagatataaaaaaattttttttatataaaaatacctaaaataatatataatatatatctatttttttttt +atatatatacatttttttatttaatgatgatgaacaTTGTCCTGCGTGCTTTTGGATGGTGAATAATAGCATCCAAAGCT +TTTCCTCACAGCTTCAAAATCAGTCATTAAACTAACATGACCACAATCACTAGGAACTTTAgctacaaaaaaaaaacctt +CAGATTTTAGGCATCAACCATTTTGAATGAATAACGCCATCACCAGGTCCATAAAAGTAATTATGATGATGTATTGACCA +TCTTTAATGTCCTTCTCACCATCTTTTCCAACACACCTCGCACTGAAGGGACAGTATTTCCGTAAACTAAAGCTAATGGT +GGATACttatctttcaattctGGATTGTAACTTAACTCGTCGAGAAATTTTTTAGTTCTTTGTAATGTACGCGAAAGGTA +TTCATAAGATTGCATGCCTTTGTGTGAAGATACTAATGGAGACAAGTTATATTTGACCCAAGTATCAGGGTTGAACAAAT +CCATATGTATGAATCTCGTAATTATCGAGAAATCCTAATGTACGCGAAAGTTCTTTGTACATAAGATTGCATGCCTTTGT +GTGATGTTTATATTTGACCCAAGTATCAGGGTTGAACAAATCCATATGTATGAATTTTTCCGTGTTTGtatcaaagaaaa +catTTCCATGTCTAGGCAAGAACACAAAGCTTGAACGCATCAGGAAATTCGCTTCAGCTGTCAAAACATTCTTACTGAGT +AATACAGAGTCCCCGAATCTTATAGGACCAAGAATGTTAAGACATTCACTAGGGGTCCCCACATATAAAATACCATTGAA +AAGCGTTGGATCTTTGTTCATTGCATGATGTGCAATTAATCCCCCCATTGAATGTGCCACAACCAACGCtcctttgtttt +ttttgccCTTTTCACCGTTcgtattattattactgttattattattattattattattattattattattgttgttgttg +ttgttgcatGGTAGCTCTTTAAGGAACGCCTCAAGCTTTGCTGAATTGATATCACAAGACAATCTCCAATCATACCCAAA +TTCATGTACTCGTACCCCGGGCACGGCCGCCATCTTCTTGATGAGTTTCTTCGAGATATCTATAGattattactgttatt +attattattattattattattattattattgttgttgttgttggttGCAATCTTTGCTGAATTGATATCAATCATACCCA +AATTCATGTACTCGTACCCCGGGCACGGCCGCCATCTTCTTGATGAGTTTCTTCGAGATACTATAGGCCCTATATTGGTC +AACATCCCATCAGGGTATATCTTTTTAGGCATGTCATACTCAGCTTGGTCATCCGGAGGCACCACCAAATCCACCTTTCT +CAAGTTGAGCCCCACCTTCATCGGGATCCAAAGCATTCGCCCTGTTTCTGTGTCTCTTAAAACAGACCCCCGATAGCCTC +CTAGCACCACTATGTCGCCCTCTAGCTTTTCAAATACCGTTTCCGTGTAATATTCATATGATTTTTCTCCAGTTTTTTCG +CTAATGAACGCCGGTGTAAGTGCCTTTTTCACCGCACGAAGTCTCGAGTTGTCTGCTCCCTTGAGCTCCCTATAAGCATA +AGCCGCCTCAAGTGTACTCATAGAATCCTGTCGCTTCAATTTCTGTCGAAGCTGCTTGTCTGTAATGTTCTGGATAACTG +CACCGGAAAATCTCAATTTTGGCATGCTAATCCTGCTGGTAGATCGTAAGTGTATCTGAAAATGGTAAAAATCtcaataa +tgaaagaatGTCTGTAAGTGTATCTGAAAATGGTAAAGTTAATGAAAATTCCCGTCGATTCTCAGCAGCTGTCTTATTCT +TAGGCGAGGAGCAAATATTGGTGTTTccattattactactattaCTGCTACTTCTACTGCTGCTTCTGCTACTAGTAGTA +TTGCTATGTgtagtattttttttcccaaaagaaataaacGATGATTTCTGTTTTATATAATTAAGTGAGCTTTCTACCAG +CATAAGACTGTTGTGTCTCGATGGTCGAGTGGCCGtgaatttcttcttttctagTTCCATAGAATCAATGATATTTCTTA +ACTTGTTACCTGCAATTATCGCGGCTTCCTCACCCTGATTTTAGCAATAGTTCCCACATTTACTGCTTtctgttgctgct +gctgtaCTGCCACTGCTTCTCTTGCATCGCCTTTCAGGATTTGTTTctctctttcttctttgttttcattttcttttcat +ttccATTTCTGTCTTCTTCTACTACTCTGCTTGTCATGTCATGCCAAGAGGAACCACTCAATCAATGCCTTTCAGATTTG +CTACTCTTTCGCGATGTTTGTCATTTTATTCGTAGTCATTATGGTCGATGTATGTCTTTGTATGGTTCTCATCATTATGA +ATATACGTCGCCATTAGGAGAGCTGTTGTAACATCATTTTGAATGTTGAAGCTTATTCGGCTTAGTTTGTTTGTTAGCCC +TATATTATGGTGATGTATGTAAGctttgattcttttatcctttttcaaaataatactgTATCAAGGGGAGTCGCCATTTT +GTATTTATCTATGGATGTTTTCTACTAGCGCTAGAGTGAACAATATGGTATCAGCAGAGAACACCCTAAATTTGGTGATT +TGTCAAACTCTATTTTCATCCTTTTTGAAGAGTTTAACTGAAACAGCAAATCGATATTCTTCATCTAGAATTCCGTTTTA +TGCATACCAATATCCAGGATGAAGCCAGTGTACTCATATGCCGCATCCGACTATTGGTGCCAACatcataaaaatatcaa +ttagGGATTGGAAATATATTTGCATCCTTCATTTTGATGACAAGTGCATGACTCAGTGAATTGATGATCAATTTAGATAT +TTTGGTGTATATTTCATGTAAAGATAGTTTCTACGAGGAAATAAATGTTGCAGTAGGTTCATTTTTAGATAAAGTCTACA +GTATCTTGTCTTCTTAATCTCAAAAATATTCCTAAGAAGAGTAGTTTCACCACCATTGTAGCTTTTTATTCTTGccatta +tttaataaaatttacCTAGCAGAGTTTCTTGCACAAATCTCATGTTTTAtgagtatttttttttatatctttCCCCCCAC +TTATTTCAGTGCCTTCTTTCTTCAAGCAttaatctttttctctatatcacaatttccaattttttcacAACCTTTTAACA +CATGCTTTTGAACAGTTTAAGTTCGATTAACATCATTATTAAGCATTGATATTCGATAGCCTATCAATATATTGCTGTTG +GAAAttgatcttgaaaattGCCCTTCTTGGCAAATAAGACGTCATATCTTGCCATACGTTTCATAACAATAAACATTATT +CAGCACAAATTAGctttttctgaattttttataaattatttacttAATCGTTTGTCAAAAGTATACGTatccaaaaaata +aataagttCAACGAAGTgcgtttttttttatagaATTTCATGAGCCAGATAATTTCAGGGCACATAGTATATACtgtgaa +gaaaaaatgggGTGTCGGATGATTTGGCTCGGATCATATGCCCCAGTATAGacaatatttttactttcatcaataatgaC +AGAGTCATGCTGTTTAGATGAATTCAATAGATTTAAACTATGTTATATTTAATCAGAGTTGTTATTAAACCAAGTTATCA +TGTCCATAAGCCGATTATcggaatttgaaattatttccTCTTTTAGTTAATCATgaaatttgatttaatttGCTTAAGTC +ATTATTCATGgatgaaatttttgttttattcaaatattaagaAGTAGTAAATAATTGttgttctctttttcttttatctt +CTAGCTTCTTATATATCAATTGCTATTTAATAACTTTTATTGTTACATTGTTTTACAAGTGATCTTAATTCATCAAGAAG +ATCATCTCATATGTAACTGTTATTCTTTTATGTTGTTTAGCTTGAGTAATACACCACAGCTGATTCAATATTACATGAAA +GATtgattatcaatttttttaagcTCAGTATGCATCTGTACATGTCAAGGGATATAAGTAATTTGGAATAAGCAGGGCCA +TgtgaaaatataaatagttAATATAATTGCTCCCAAATTTGCTTGATAATTACAAGAGCATGAAGGAAGACAGATTAGTA +TATAATATAGTCCATTTTCATCAGGTTCTGATGAAATAATGTCAAGATAACATAGCCATATcctaataaataaaaatatg +aagaatataatagtaatagtataaaatgataacaaacgaacatataatatgcatgAGAATAAACTACGCGTGATTCAATA +TATGCAAGATTAAGACCAGTAGAAAGCAGGTGAATATTACGTTTCATACgacgaaaaaattaatgcaGGGCCATATAACA +GGtgaaaaatccaataacaataatacgaataatatcaagaagatataagaaaaagaagaacaatACATTACTTAATATAG +TTACTTAATACAGTACCATCATTAAATCAGAATAAACTAATCGATTTATAAAATCCTAGAACGTCCCAAAAAGAGAGCAC +ATTCAAATCTTATAATATACGAAaccttattaaatttgaatgcgatatattgaataaatattaaataataaatatacaac +attaacaatattaatacttagagaattaataaaatctgaaaaagctatttggatttaattattaaaaaagaacttacagt +attcattaaatcaTCTAAAAAAACTGATACTCGTTATTTATGAAAGCAAGATCCTAAGATAATCCGAAAATGACTCCTAC +CAAagtcaagatgaaaagcgACTCGGACAATGATTCAGTTTGAATTCGTTAATTCCAATCGGTCAAGTCACTCTGATCGA +ATCATtcaattagaagaagagtCTAAATCCATGAAGACCGAGTTTAAATCTGTAAAAATCTGAATTTAAAGCTATGGAAA +TTGGATTTAACGAGAAATTCGACAGAATCTTAAACTATTTATATgctctaaatttatctaaaaaatatgatactgttatt +attgatgaaagcaAGAGTAATGTTTGTACTAGAtcctaaccaaatcatTAGAAACACTAACACTAAACTAAAACGTtaaa +gagaagaaaaggaagatGCATATAtgtcaaatatatttaaaggcttttttatttatatgctctaaatttatctaaaaaat +ttaaaccAAATCATTAGAGATTTTAAGAATCCTCGTTTTAGTTAGGTATGAGTATTAATAGTGGTTCTGAAAATAAGTAA +GAATAGCTTCTAATTCAGGGAAATCTGTCGTTTCGACATATGTAATTGATCCATCTTTGATATGTACTTTCTTGTATAAG +GAGGCTACATTAAAGGTAATTAGGTACACAAAATCTATATTAGTTATAAGACTAAATACATAAATCATTTGTCAAGGCGT +AAAGGCTTTTTGACTTTTCAGAGATTTTTCCTCGTTCTCAGAATCACTATTAATGTTAAGGATCATCCTTAATTTGAGAA +AGATAACCTATATTcttatacatatatatattatatatcttTCTTGTGTGATATAATTTTAAGATGACTCGAAAATGTGG +ACTATTTACATTCTTGTTCTCACTGGCCAATTTTCACATTACTTATATTCTTGCTATTTATAAACATGCTACCATTATCC +ATAAATGGCTGATATTGATGACTTATCACCTCTAATATGGGCTATTTTGGCATTAGCTTTTTTTGTCACGTGATACGACA +ATATTATATCCCGACAATAATACTTAATCAAACTTCATGTAATCTTGGGCCAGTCTTACAGCATACTATCGGCTCAAGAT +TATTAAATAGTAGCATTAAGAAGCTTGCAGTTCTTAAGATATTCTTGCATATCATATAAACATGCGAAGTTATCCATCCA +TCCGCATAATATAGAACTTTTCTCTCTCAAATTGCCGAACAGGATATATCAAAGATAGCTCAGCCATATATAAGAGAACA +TTTATATAACGAAAAACTGTTAATAAAGTAAGTTGCtgttaataaaaattggtatataagaagctcGCATTTACATAGGA +TATTCTTAGATTATATCTCACAAGAACAGTTATACTTAATGTCGGAACAAGATATTGTCAGATCACGTGGACCAAAGTAG +GTTAGCCGAAACATACATAATAATCATGACAATATTCGCATAATGGTAGCACATGTAAAATGAGAATCGAGAACGCAATA +TATAAGTAGTCCGCATTTCTCAGAAGTGTTTTTACATTTATATGTTTACATTGgaatatacatatatacaGATGTAGAAG +ATAAttatatatcaaaaaacaTATTTATTCTAAGCTCTCTCCCAAATTAACAGGTGTAATAACACTAATCAAAGAGTACT +ATAACTCAAAGTTAAGTTGAATGAACTTGGCTCaagtttaataataaatatttgcGTTATTAGTTATAATAATGTGTATA +TAAGAATTGCAGATTCTCCGATACATCCTTTGCTGAGCCAGCAATATGCTGTAGAACGACTTAATACTTCGACTCCAGGA +ATAGCTCGGGCTTCAATTTTGACATAACCgatctaataataaaatttgcgttattaaaaatatacattTGAAAAGGTTAT +TGACCAGCAATTTCTTGGatttatcttttaatttGGTATGAATCAAAATTAGACAGCgttattttattgtttcaTAGGTA +AGCACTCTTGCGAAATGGGTGCCAGCACCTGCAACAGCCTAAACTTAGGTCTCCATGAAGATGGTACTGCATGTTAGACA +GCGTCTCCATGctaaaatataaaaaaaaaatatacatttGAAAAGGTTTTTATATATGTGACCCTAGaaaatgctttttt +ttacttttaatttGGTATGAATCAAAATCTCATAGGTAAGAGCTGAGGATATGTCCAAAAGTTTGTAAAGTCGCAAAGAT +GTGTAAAATGATTcataaagaagaaaactAAACTTTAACCAAGAAAAGGTATTTAAAGAATACATTAACGTAAAAatact +tgaaaatttagaaatacAATCCGTTATTGTCTTTCATGAGgaagaatatgaaaatttgaagcTATTGGTCATATTTTAAA +TGGCATATCGGACAATAAACTAAAGTTAAATGAAGATATCTAGAGGTTTAATTATGgtacaagaagaaaataaagaaaaa +gtatgcgatataaaagaaatgtaTGGGCGAAATATTTGACACAAAATTGAACTCGGAAAACCTCATTGAAGTATAAAAAG +ACATAACTGTTGAGGTTATAGAATCTTTTTGGCTTTGCGCAGATTCTTACTCTTTTTCAGAAACACTACTAATTTTGATA +ACTATTTTTGATTCGAGAGAGATATCGTATATAATTTGTATATACTATATATCTTCGTTGCCTTCCTATCTTTCTTATAT +GATATATTTCTAGGAtccttttgaatttctggaatatttatattgttgTTCTCACTAGTAATTTTACCTGTTTCCTATTA +CTGTTAAGTAATTTATACACTTTCATAACTAGTAAAGGCGTTATGTAAATCTATATCTTTGGTTTAGCCTCTTTTTACAA +GGCTTAATTTGTTTTGCGTGATCTGGCAATATCATTCCGACAATCTTACGTAATCAATCCCTGGGTAACCTTGAGTCAGT +CATACAGTGTATTTTGGCTCAGCAATTAAGGATTACCgttaaattttttaaaaccAAAAATATTGCAAAAGCCCTGAATT +CCAAAAAGTTAGTCAGCAGATTTAAACTAAATATGTGCTAATAAGTTGTCAAAAGTGAACAACTGATCTTCAATGATTCC +AATTTTTAAGTATATGAAATTTCATAGGTTTTCCATAATAAACattcatttctttaataaaaaaattgttaagCCAGCAA +ACCATTTTAGGACTGACTATTAACATTAATAGTGATTCAGACAATGAGGATGAATCTCTGCAAAGCCAGCAAGAGATTAC +GCCTCAACACGAGCCAATGTTTTTGTAGCCGAGCCCTGCCAGGGAAAAATCCTATTATTGCTATATGCTGAAATTAGGTC +AAttgttcatttttttttatttctaaatttgCTTTTTATATAGAAGGTTCGAGTGCAAATATTGTGATTGTTTAACAGAA +AGAGCTAGCACATATTAAGGAATGATAGTTgtttaattaattattaatcatGTACTTATAATATCACGTGATATGTGCTT +GAGGTTTGCCCCGCGACGCACCATTTGTGGACAACTCCCCCATAGCTACAAATTTCACTCTGGGCTGGAACTTCTAGCGT +TTTGCTATATCCCTGTTTAGCGCAAACAAGAACCTAAGAGGTTTAATTGCAGTATAAATCTTATAGTTCAGGCAGATAAC +ATTGCATGTTGGGCAGCGTTTTCACGCCTagacgaaaaaaaaaattatcatgaACAAAACAAGTGTGTTTTTGAAGATAG +GTTTTCTATATGCATCCCTAGGAAGTATTTTTTCATGCTTGCAAATAGGGTTAAGTCTACTCTCATAATTAATATCTGAG +AATATTactgaaaatttaaaatgtGTCAAATAATGAGCAAACTGCCTTCAGAGAAGAaactttaaaatttatattaaata +atataagaaTATATGAAAACTCCATAGAAATAGTATAGAAACTGAAGAAATCTGATCTTTGCTAAAAATGCGTTCTGTGT +AAGCAATAGCGAGATTATGAAAACTGTCAAGATATAATCGCCAtatcctaagaaataacaatatgaaaaatataatatga +ctatcaaaatataatcGCCATATCCTAACGAACATAACAATATGCACTACGACGCGTATATATGCATTATAAAATTAGAG +CATAacgaacatataatatgcaCTACGACGCGTATATATGCAGGTATAATCCCAATAGGAGGAAGATGAATATTACATTT +CACGACGAAAAAGTTAGAATAtaacaagtgaaaaatccaataaaaataatacgaataatatcaagaagatataagaaaaa +gaaggaCAACTACATTACTTGATATTTACTTAATACCaaataaagagaataaCTACTAgtctatttataaaatccaAGAA +CGTCCCAAAAAGAGAGCATATTccaattttataatatacgaaaccttattaaattttaatgtaatatattgaacaaatat +tgaatattaaataatgaatatacaacattaaataatgaatataCAACATTTGCAACATTAATACCTATATTACTATTTCA +TGAATCATTACAAAAGAACTTATAGTATCCATTAATTAGATTATAGGCAATCCGAAAAATGACTCCTAGCAAGGTCAAGA +TGAGAAGCGATTCTGACAACGATAGTCTTCaaatccaatttttcataatcattCTAATCGGATTACTCAATTAGAAGTGG +AAATTGAATCTATGGAAACTGAGTTCAATAAGAAATTCGACAGAATCTTAAATTGTTTATATGCTCTAAAAATGTTAATG +TGAATTTAaagttttttcaatgaagTCAGTTTGATTAATATTTCACAAATAGCTTTAACTGTTTAAATTTTCAGGCATGT +CCTCATCCCTTAACTAAATGAAATGACGATAAACCATtagaaagaattttttaaagaCTAATAAGCTATGCTACCTTTAA +AAGgtataatattgaaacttGCCAAAGagtatatatttttataaattaaaaattgaagaaagaataaGCGATGATTTACT +ATATAGATATAAAACTTATATAACAAAGAATAGATTAGATATAAAACTTATATAACAAAGAATAGATAGTGATTATTGTT +GAGTCATGTCTGACTGGCTTAACTCTGGGTTAAGTTACTCTCTTGATTAATGTTATTCTTCATACTTATGTGAGAGAGAC +AGGTTgttgttatatttttgatatatgtATCTATATCTACGTGTATCTATATTGTCCATCTGTTGTGAGagatataattt +aataatagcTCGAGGAAATGTAGACTACTTATATTATCGTTCTCACTAGCCATCTTGTATCGCTTACAGTTACTATTTAT +TAAATGCGTTGCTATTTTATAAATGGACATGACAGTAGACGATGATCACCTTTGATATAGCGTATTNNNNNNNNNNNNNN +NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN +NNNNNNTGAAACGTAATATTCACCTGCTTTCTACTGGTCTTATACTTGCATATACACGCGTCGTcatgcatattatatgt +tcgtttgttatcattttatactattactattatattcttcatatttttatttattaggATATGGctattatatcttgaca +TTATTCATTTCAGAACCTGATGAAAATGGACTATATTATATACTAATCTGTCTTCCTTCATGCTCTTGTAATTATCAAGC +AAATTTGGGAGCaattatatttctatttatattttcacATGGCCCTGCTTTTCCAAATTACTTATATCCCAACATGTACA +GATGCATACTGAgcttaaaaaaattgataatcaATCTTTCATGTAATATTGAATCAGCTGTGGTGTATTACTGGCTAAAC +AACATAAAAGAATAACAGTTACATATGAGATGATCTTCTTGATGAATTAAGATCACTTGTGAAAACAATGTAACAATAAA +AGTTGTTAAATAGCAATTGATATATAAGAAGCTAGaagataaaagaagaaagagaacAACAATTATTTACTACTTtctta +atatttgaataaaacaaaaatttcatcCATGAATAATGACTTAAGCaaattaaatcaaatttcATGATTAACTAAACgga +aataatttcaaattccgATAATCGGCTTATGGACATGATAACTTGGTTTAATAACAACTCTGATTAAATATAACATAGTT +TAAATCTATTGAATTCATCTAAACAGCATGACTCTGtcattattgatgaaagtaaaaatattgtCTATACTGGGGCATAT +GATCCGAGCCAAATCATCCGACACcccattttttcttcacaGTATATACTATGTGCCCTGAAATTATCTGGCTCATGAAA +TtctataaaaaaaaacgcACTTCGTTGaacttatttattttttggatACGTATACTTTTGACAAACGATTaagtaaataa +tttataaaaaattcagaaaaagCTAATTTGTGCTGAATAATGTTTATTGTTATGAAACGTATGGCAAGATATGACGTCTT +ATTTGCCAAGAAGGgcaattttcaacatgtaTCAATTTCCAACAGCAATATATTGATAGTGCTATCGAATTTTATAATGC +TGTTAATAACTTATCCGAAGCTGTGTTAAAAGGTTgtgaaaaaattggaaattgtgatatagagaaaaagattaaTGCTT +GAAGAAAGAAGGCACTGAAATAAGTGGGGGGaaagatataaaaaaaaaaactcaTAAAACATGTCATTTGTGCAAGAAAC +TCTGCTAGGTAAAACATTGGATGGCAAGAATAAAAAGCTACAATGGTGGTGTACTACTCTTCTTAGGAATATTTTTGAGA +TTAAGAAGACAAGATACTGTAGATTTTATCTAAATAATGAACCTACTAACATTTAATCCTCGTAGAAACTATCTTTACag +aaatatacaaaaatatcTAAATTGATCATCAATTCACTGAGTCATGCACTTGTCATCAAAATGAAGGAGCTGCAAATATA +TTTCCAATCCctaattgatatttttatgatGTTGGCACCAATAGTCGGATGCGGCATATGAGTACACTGGCTTCATCCTG +GATATTGGTATGCATTGACGGAATTCTAGATGAAGAATATCATTTGCTGTTTCTGAGATTAAACTGCttcaaaaagttga +aaataagTTTGATTTGTATTTAAGTGTTCTTCTGCTGATACCATATTGTTCACTCTAGCGTTTATTAGTAGCATAAAACA +TCCCatagattaaataaaaaaatggcGACTCCCCTTGATACAGTACCCACTCTGAAAAAGGATACAAAAGAATCAAAGCT +TGACAAATCACCAAATATAGGGCAAAAACTTGAGACTAAGCCGAATAAGCTTGACAACATTCAAAATGATGTTACAACAG +CTGAGCAATCCTATTATTTTGGCGATGATATTCATAATGATGAGAACCATACAAATACATACATCGACCATAATGACTAC +GAACTAACAAGACAAATATCGCGAAGCGTAGCCTCAACATTGATTGGTGGTTCCTCTTTTAATGACGTATATGACAATGA +CCAGGGAGTagtagaagaagaggaagaaaatggaaatgaaaatgaaaatgaaaacaaagaagaaagagagAAACAAATCC +TGAAAGGCGATGCAAGAGAAGCAGTGGCAGtacagcagcagcaacagaAAGCAGTAAATGTGGGAACTATTgctaaaatt +aaaaatcaGGGTGAGGAAGCCGCGATAATTGCAGGTAACAAGTTAAGAAATATCATTGATTCTATGGAActagaaaagaa +gaaattcaCGGCCACTCGACCATCGAGACACAACAGTCTTATGCTGGTAGAAAGCTCACTTAATTATATAAAACAGAAAT +CATCgtttatttcttttgggaaaaaaaatactacACATAGCAATACTACTAGTAGCAGAAGCAGCAGTAGAAGTAGCAGt +aatagtagtaataatggAAACACCAATATTTGCTCCTCGCCTAAGAATAAGACAGCTGCTGAGAATCGACGGGTATTTTC +ATTAACTTTACCATTTTCAGATACACTTACAGACATTCTACCATCAAGGATTAGCATGCCAAAATTgagattttttcaaa +acgATGACAGCAGTTATCCACTTCCGGTGCCCGAGAACATTACAGACAAGCAGCTTCGACAGAAATTGAAGCGACAGGAT +TCTATGAGTACACTTGAGGCGGCTTATGCTTATAGGGAGCTCAAGGGAGCAGACAACTCGAGACTTCGTGCGGTGAAAAA +GGCACTTACACCGGCGTTCATTAGCGAAAAAACTGGAGAAAAATCATATGAATATTACACGGAAACGGTATTTGAAAAGC +TAGAGGGCGACATAGTGGTGCTAGGAGGCTATCGGGGGTCTGTTTTAAGAGACACAGAAACAGGGCGAATGCTTTGGATC +CCGATGAAGGTGGGGCTCAACTTGAGAAAGGTGGATTTGGTGGTGCCTCCGGATGACCAAGCTGAGTATGACATGCCTAA +AAAGATATACCCTGATGGGATGTTGACCAATATAGGGCCTATAGATATCTCGAAGAAACTCATCAAGAAGATGGCGGCCG +TGCCCGGGGTACGAGTACATGAATTTGGGTATGATTGGAGATTGTCTTGTGATATCAATTCAGCAAAGCTTGAGGCGTTC +CTTAAAGAGCTACCatgcaacaacaacaacaacaataataataataataataataataataataataacagtaataataa +tacgAACGGTGAAAAGggcaaaaaaaacaaaggaGCGTTGGTTGTGGCACATTCAATGGGGGGATTAATTGCACATCATG +CAATGAACAAAGATCCAACGCTTTTCAATGGTATTTTATATGTGGGGACCCCTAGTGAATGTCTTAACATTCTTGGTCCT +ATAAGATTCGGGGACTCTGTATTACTCAGTAAGAATGTTTTGACAGCTGAAGCGAATTTCCTGATGCGTTCAAGCTTTGT +GTTCTTGCCTAGACATGGAAatgttttctttgataCAAACACGGAAAAATTCATACATATGGATTTGTTCAACCCTGATA +CTTGGGTCAAATATAACTTGTCTCCATTAGTATCTAACTCACACAAAGGCATGCAATCTTATGAATACCTTTCGCGTACA +TTACAAAGAACTAAAAAATTTCTCGACGAGTTAAGTTACAATCCagaattgaaagataaGTATCCACCATTAGCTTTAGT +TTACGGAAATACTGTCCCTTCAGTGCGAGGTGTGTTGGTCGATGGTGAGAAGGACATTAAAGATGGTCAATACGATAATT +ACTTTTATGGACCTGGTGATGGCGTTATTCATTCAAAATGGTTGATGCCTGAAAATTATGAAGGTTTTAAAACTGTAGCT +AAAGTTCCTAGTGATTGTGGTCATGTTAGTTTAATGACTGATTTTGAAGCTGTTGGAAAAGCTTTGGATGCTATTATTCA +CCATCCAAAAGCCTTGGATGCtgttcatcatcattaaataaatacatgtatatatataaaaaaaaaatagatatatatat +atctataacagtatagatatatatatatctattttttttttttataatttttttataatttttttatagtcttttctatc +atttttttagcTTTGAGTTCAAATTgtaatgattatttttggGAAATAATTAGCACGCAGGACAATAAAGTTTATGCGCA +AAAAATGTGTACAGccaaaaaaattctaatttatttaagtattatttattatatagtTGCTTTATGCGTCATAGTAGATG +TCAAATTAGgattaaattttttgctTTCCCACTTTACTGGAGAGTATAGAAAACTTTGAGAACATCGGCTGTTTAAgatc +taatttttttgattttctagGTTTTCCATATTGAGTTTTTTTTGAGTTTTTTAATATGTCAAATGGTAGTGCGACCAGTT +TTGTATCACAATGAGCATTAATCTTCAATGTCGATTCTAATATTGTGTTCTCCTTTCCTAATAGTGAGCTTGCACATAAA +TCAAGATACTCTTTTCTagatttcattaatatgGTTTGAGGTTCAATTTGCTCTGCAAGGTATGATTTCTCAGGAACTTC +ATAGACAAAGGTACCTTTCATATTTAAGTGCTTCTTATTTATggttctttttgttttgaatcttaaaaaatcattatcaa +atatctGCTGGTGAAGATTATTGTCGAATATCGCAagataatcaaaataacTCACAAAGTTCTGACCTGAGTTTCTCCAG +ATATCTGAAACTATCTTTGATACCGTTTGGCTATTTATCGCTGTCTCTTTGTATCTTAATaaacaattaataaaactTCT +TGCTATCATGAATTTATTCCTAGGTCTTGTTggttcttcttttctattattatttttattactcTTTTTCAGTATTACGG +TAGATACAATAACATCGCAATTAACTGCAATAACATTGTATGTTTCATAACTTGTATGTTTTTTGGTGGTTCTATCCATA +TAAGGAAATCCAGTAACAGCTATATTgttatctttatttctatttacTTTAGGTAATTGGAAAGAtagtattttctttga +caTATTAGGTATTGGttaaattttaatgaaCAGTTCTAATTTGTGATATAGTATATAGTATTGGAATAATTTAGTGATtt +aaaatgataaagaaaatttttgaaataatttatttttgaaggaaaagaaggttattataaataaaaaaaaaatatatata +tgcaagaaataaaaaaaataaaaaatatgaaaaatatggtATCAAATGTGATTCATAAAAAACGATTGATCccaatttga +atttttatcACGTGTCTTTGCACATCTTTCTCATAAATCTTGGTTTTGgttgttttttcttgacTGATCAAATATATGGT +ATTTAACTATAGTTTGCTTTTTTGCTTCCTAACttcatataaatattattgtttccTCTTAAGTTTATTTCTAATTATcg +aaaaaagtaaaaaataacTCATAAATATACTGCAACATGAgtgataatgaaaagattGCTATTACGGTGGGAGAAATGGT +CGCCAAGTTAAGAGACACGGAGCAATGCATCATACGTACAACTGGTAGAACAAGTGCTGACATCTCCATTTCTGATTTTA +CAAAAGATACAAATAGCTATGTCCAGTATTATCATCTTACAGAGTCTGAACATATTCCAGAAGAGTTGAAATTAAGTTAT +AAGCCATTGATTGAACGCTATCAGACTTATCTCAACACAATAATCAAGGTATGCCATACTTTCTTTCtcttatattattt +tatgaGACATTTACTAATTAATTTAGTTATCGGATGCAGCAATAAAGAGTGGATTCAAAGTGTGTGATAAGGATTCAgtt +tataataaaacagAAAGGATGTTGATCGTCGACATTTTCTTATCACAATCCACTCTCAAATCCAGCAAATATACAGATAA +GTTAAGAAAGTATCTTGAAGATAGAGGTGTAGATATAGTTGTTTTAATGCATGACCAGCTGCAAACGTCTAATAATTCGA +GATACTCTAGGAAAACTACTGAATATCTAGAACTTCTTTTCGATACAAATCCTAGACCTAATTCTCGTGAAAGATTATTC +ATAGCGAAGCAATGTGGATTAAGTGCCAAACAAGTCAGAATATGGGTGagtattttattattattattattattattatt +attattcttcttcttcttcttcttgttgttgttgttttatacaaatatatatatatatatatatatatatatattatttg +tgTGGGACGGGGGTGGAGGAGGCAGCAGCAACCtgaaatttatatttttatttggcAAGCAATATCTAGAACTAACAAGT +ATTTTTATAGTTTACCAACAGACGTGCTAGGGTCAAAAAGACACAAAAGCcatagaataataatttattattacgtaaat +atatatatataaagagaaagagagagaataaataataaactttaatctttgtttttcttgtttttcttgttttttttttt +tttttttttgttttttttaatcGTATGttgtaatatttctttctttaaataaattttttattgagTGATATACTGGAGGA +GTAAGAGtgtaattttaatttaattaatatatctaatattcttcatcattatcgctatcatcatcatcatcttgataact +atattttctaatatcaCCTAATCTCTTTCTAGCAGCACCTAAGGCATTCTCCAACTTGATGATTTCTGCTTGttgtttca +tttcttcttttctattatcAATGATACTCAACTTGGagaaatcaatatcatcattaacTAATTGTGTagatttattaatc +aattctTGCACTTGGCTTACTAAGCTCTTGCAAGCAATAGACACCTTCTTACTTGCATCTTCCAAATTGTCTTGAGTCTT +GGAGAACTGCGTAGCTTTAACTCTTGCAGCTGCCACTAGCTGAATAGTAGAAGATGCAACTTCATTAGATGCCACAATCA +ATTCCTCGTGTGTATTCTTACCACTCAAATTACCATCCGCAGTTTGAATTAATAAGTTGGTAGCAGATGCAACAGCTTTA +GCAGCAGAGATTAATCCTTCTGTCCAtctattattctttttgtagAATTGGGCTCTTGTACTTGAACCTTTACCATTTGC +AACAATCTCATGTTGAGTATCGATGGCTGCAGAGATTAACATCGAAATAGCTTTGGTGATAGCAATGGATGCTGCCAAAA +TAGCCTTATTGACTTCTAAGTCAATAGGTGACAAGTCCTGTAAGTTTTTGCTGGTTAGCAACGACGTCAAGTGTAATGTA +GCTTGCTTGATAACCTCCGCAGCAGCGTTCATTTCGTTGTCCACCAAGCTTTCTAACTCCTCTTCTGTTTGTTTTTCGAT +CTCAATGAACTtcttgttgctgttgctgctgctacTGCCACCGCCGCCGCCGCCGCCGCCACCGCTCGTAGATGCAATAG +TCTCAATCAATGTAGTCAATTCTTCTAATCTTTCATGGATTTCAACGACACCTTCAATAACTTTGTCTGACTTTTGTTCT +TCGCTCAAGCTTTCTAAATTCTTAGACAATAAATcttccaaaaattcaatgCATGTCTCACCAACCAATCTTGCAGATGA +TAATAGCGAGTTTTCCAAATTCTCTGTCTTGCAAAACTTAATGATGCCTTTAACATTCAACTCAACATCTGTAATACACA +GAGTGAATTCTGAAATAGTAGAAATGATCTCAGCATAATCACCGCTTGGTCCATCAGCGATAAAGTTATTGAAAGCCGTA +GCAAATTCTTGACTTGTTTCGCCtgctttttcaataatggtTAGCAAGTATGATGGTGATGAGTTTTGATTACCGCCTTG +CATCGAAGAATCAAGCTCGAATAAACTATCTTCGATTCGTTTCACACCGCTTTGCAAAATGGAATCTATAATAGCAACTA +GCTTCATGATATGTTCTTGTAACACTGTATCAATTTGTTCATCGATAGCTTCTTCGCTTTCCCTTTTAGACTCGGCTAAC +TGTTTTAACGCATCATCCATTGTCTGTTGTAAAATTTCGATTTCCTCATCCTTTTCACGCAATGTTTCCTGTAGCTCAGC +ATTTGACTCTGGTGACATCTCTGAGATTTGCGattctctttctttcaatttggTTTCAAGTTCTTCCATTTCTTTTCGGT +GTGCGTTGAAAATGGAGTTCAAATTAGACGACTGGTTTCTTTCTGTCACATCCAACTTATCCTTCAAAGTATTAACTTCG +GCTTCTAGTTTTTCAATCAAAGAGTCCTTAGACCCTCTTAACTTGTCAATCTCCAACCTAGCTCTGTCACGTTCTTTAAT +CAAATCTGCCAATTCAACATTCTTCGCCTTCAAATCTTTCTCAATCCTGTCTCTCTTATCAATAGCCTCCTGTGCAGATG +CAGCCTTTTGTTGCaactttttgaatttttgcaataaattcaaatgtTCTTGTCTCAATTGCGAGTACAATTTTGCTATG +CTCTCATATTTGCTCTTGAACAAGTTCAATTGTTCTTGGACACTATTAAGACTTTCATCTTTCAATGCAATCTGTTGAGT +AGCAGCTTGATTGGCATTCATCGCTTCCTGTTCCAAAACTTTCACCCTTTGATCATATTGTTGCAACAACAATTGATCTT +GATCGTACTGGTTCTTTAGAGCCAATAGATCGCGTTCTAGTTCAGCTACTCTTCCTTGAGCCGCTTGTGTATGATATTGT +TGTAGCATTTGTTGCTGTGCCAtctgttgctgttgctgcaATTCGCGTTGTTGCTGCTCAAATGCAGCTTGCTGATTAGc +ttgttcttcttgttgCGCTAAGAGTTGCGCATTTCTTTCTCTGGCTAATCTTTCTTGTTCAGCTTCCACATTGTCTTGTT +GAGCTTTCCAGAATTCAACCCCAGTAGGTTGATTGATTAAAGGGCTTGCTATTTCCTTTGGTGGCGGTGGTGGCGGACTT +GCCGTTGGACTCTTCGCCGCCGGCGCCTTGGTAGGTGGTCTACGAATACCCGTGTTCTCATCAATGAGCAAATCCGGAGG +ACTGGTCGTCAACTTTGGAATGTTGATCAAAGACGTCAAATAGTGAAGGCTGTAGGAGTTACAGTAATTCCAATGAATAA +CGATTATCTTATTAAAGCTATATAAAAACTAATTAACTaagattataataaatactGAAAGGTGGGAATTGCAACCCTTT +ATATATGAGTTGTGGGTGGCAAAAATATAGCAAAAACCACAGTCACAACTAAGGTATAACTATAGGAGAATATCCATATG +GAAGTATATGATGATGTAACAATGTGCAGGAATATTCTTGTAACAATGTAAGTTTCATAGTCTTTTAGAATCTTCTAGAA +CTTTCTGGAATTCTATGAAACTTTCCGGAATATTCCTGGAGTAACAATTGGTAAAAATGATTAAGTAgagaataacaata +atgataagtataatttgaataactGTTGAATGATTGGTAATAAGTAATAAATGATTACCAATCCGAATAACAATGGATTT +TCACAAATACTGCATCAATTACTATGATCAAACAACATCAGTACAACAAGCGCAATAGACATTTCAACATTCGTCTTGCC +TACTGTCGACAATATTATAACATGAAACAACTCCATTTAGCACATGTCCCAACGGAAGCTCAACTAGCTGACATCCTTAC +CAAACCATTAACTACTATCCCCTTTTCCAGCCTAGTGCCATCTCTTGTCAAATAACATAAGTTTagtttattcaatttca +cttcaaaaaaaaaaaaaaaaaaatataaattatgtcgaattatagataatactatgaggcTTTTTACAActgattactaa +gaatgtgccttatgattagtaagaatgtgccgtgatgctactaatacaaagccgtttaatcattagattaaataatcaag +gagaaatgaaactactattatatataaagaacctaAATCCACTTTCTGCTTATATATTTCgtcattttattatttatttt +aattccttttaatcaagaatcctacttattaattattgatatacaaattgtatcatttatttattctcaacaatgtttta +tatttaatatattttctctattacatacaaaagtaaactccaatcacttctgctccttcaagtgatttacttttaacaat +cttattctCGAGTTACCTAAACAGTCATTCCGATTGAATCCTTTATATATTAGTTGTGGGTAGCAAAAATAGAGCAGTCA +CAAGTAAAGCATAACTATAAGAGAATAGCCATATTGTAGTTTATAGTGATGTAACAATGTGCAGGAATATTCTTGTAACA +ATGCAAGTTTCATAGTCTTTTCGAATCTTCTAGAACTTTCTGGAATTCTATGAAACTTTCCGGAATATTCCTGGAGTAAC +AATTGGTAAGGATGATGAAGTAgagaataacaataatgataagtATAATTTGATTAACTATTGAATGATTGGTAATAAGT +AATAAATGATTACCAATCCGAATATCAACGGATTTTCACAGTTCAAGAGCAAATATGAGAGCATAGCAAAATTGTACTCG +CAATTGAGACAAGAacatttgaatttattgcaaaaattcaaaaagttGCAACAAAAGGCTGCATCTGCACAGGAGGCTAT +TGATAAGAGAGACAGGATTGAGAAAGATTTGAAGGCGAAGAATGTTGAATTGGCAGATTTGATTAAAGAACGTGACAGAG +CTAGGTTGGAGATTGACAAGTTAAGAGGGTCTAAGGACTCTTTGATTGAAAAACTAGAAGCCGAAGTTAATACTTTGAAG +GATAAGTTGGATGTGACAGAAAGAAACCAGTCGTCTAATTTGAACTCCATTTTCAACGCACACCGAAAAGAAATGGAAGA +ACTTGAAAccaaattgaaagaaagagaatCGCAAATCTCAGAGATGTCACCAGAGTCAAATGCTGAGCTACAGGAAACAT +TGCGTGAAAAGGATGAGGAAATCGAAATTTTACAACAGACAATGGATGATGCGTTAAAACAGTTAGCCGAGTCTAAAAGG +GAAAGCGAAGAAGCTATCGATGAACAAATTGATACAGTGTTACAAGAACATATCATGAAGCTAGTTGCTATTATAGATTC +CATTTTGCAAAGCGGTGTGAAACGAATCGAAGATAGTTTATTCGAGCTTGATTCTTCGATGCAAGGCGGTAATCAAAACT +CATCACCATCATACTTGCTAaccattattgaaaaagcaGGCGAAACAAGTCAAGAATTTGCTACGGCTTTCAATAACTTT +ATCGCTGATGGACCAAGCGGTGATTATGCTGAGATCATTTCTACTATTTCAGAATTCACTCTGTGTATTACAGATGTTGA +GTTGAATGTTAAAGGCATCATTAAGTTTTGCAAGACAGAGAATTTGGAAAACTCGCTATTATCATCTGCAAGATTGGTTG +GTGAGACATGcattgaatttttggaagATTTATTGTCTAAGAATTTAGAAAGCTTGAGCGAAGAACAAAAGTCAGACAAA +GTTATTGAAGGTGTCGTTGAAATCCATGAAAGATTAGAAGAATTGACTACATTGATTGAGACTATTGCATCTACGAGCGG +TGGCGGCGGCGGCGGCGGCGGTGGCAgtagcagcagcaacagcaacaagaAGTTCATTGAGATCGAAAAACAAACAGAAG +AGGAGTTAGAAAGCTTGGTGGACAACGAAATGAACGCTGCTGCGGAGGTTATCAAGCAAGCTACATTACACTTGACGTCG +TTGCTAACCAGCAAAAACTTACAGGACTTGTCACCTATTGACTTAGAAGTCAATAAGGCTATTTTGGCAGCATCCATTGC +TATCACCAAAGCTATTTCGATGTTAATCTCTGCAGCCATCGATACTCAACATGAGATTGTTGCAAATGGTAAAGGTTCAA +GTACAAGAGCCCAATTctacaaaaagaataatagaTGGACAGAAGGATTAATCTCTGCTGCTAAAGCTGTTGCATCTGCT +ACCAACTTATTAATTCAAACTGCGGATGGTAATTTGAGTGGTAAGAATACACACGAGGAATTGATTGTGGCATCTAATGA +AGTTGCATCTTCTACTATTCAGCTAGTGGCAGCTGCAAGAGTTAAAGCTACGCAGTTCTCCAAGACTCAAGACAATTTGG +AAGATGCAAGTAAGAAGGTGTCTATTGCTTGCAAGAGCTTAGTAAGCCAAGTGCAagaattgattaataaatctACACAA +TTAgttaatgatgatattgatttctCCAAGTTGAGTATCATTgataatagaaaagaagaaatgaaacaaCAAGCAGAAAT +CATCAAGTTGGAGAATGCCTTAGGTGCTGCTAGAAAGAGATTAGGtgatattagaaaatatagttatcaagatgatgatg +atgatagcgataatgatgaagaatattagatatattaattaaattaaaattacaCTCTTACTCCTCCAGTATATCActca +ataaaaaatttatttaaagaaagaaatattacaaCATACgattaaaaaaaacaaaaaaaaaaaaaaaaaaaacaagaaaa +acaagaaaaacaaagattaaagtttattatttattctctctctttctctttatatatatatatttacgtaataataaatt +attattctatgGCTTTTGTGTCTTTTTGACCCTAGCACGTCTGTTGGTAAACTATAAAAATACTTGTTAGTTCTAGATAT +TGCTTgccaaataaaaatataaatttcaGGTTGCTGCTGCCTCCTCCACCCCCGTCCCAcacaaataatatatatatata +tatatatatatatatatttgtataaaacaacaacaacaagaagaagaagaagaagaataataataataataataataata +ataataaaatactCACCCATATTCTGACTTGTTTGGCACTTAATCCACATTGCTTCGCTATGAATAATCTTTCACGAGAA +TTAGGTCTAGGATTTGTATCGAAAAGAAGTTCTAGATATTCAGTAGTTTTCCTAGAGTATCTCGAATTATTAGACGTTTG +CAGCTGGTCATGCATTAAAACAACTATATCTACACCTCTATCTTCAAGATACTTTCTTAACTTATCTGTATATTTGCTGG +ATTTGAGAGTGGATTGTGATAAGAAAATGTCGACGATCAACATCCTTTctgttttattataaacTGAATCCTTATCACAC +ACTTTGAATCCACTCTTTATTGCTGCATCCGATAACTAAATTAATTAGTAAATGTCtcataaaataatataagaGAAAGA +AAGTATGGCATACCTTGATTATTGTGTTGAGATAAGTCTGATAGCGTTCAATCAATGGCTTATAACTTAATTTCAACTCT +TCTGGAATATGTTCAGACTCTGTAAGATGATAATACTGGACATAGCTATTTGTATCTTTTGTAAAATCAGAAATGGAGAT +GTCAGCACTTGTTCTACCAGTTGTACGTATGATGCATTGCTCCGTGTCTCTTAACTTGGCGACCATTTCTCCCACCGTAA +TAGCaatcttttcattatcacTCATGTTGCAGTATATTTATGAgttattttttacttttttcgATAATTAGAAATAAACT +TAAGAggaaacaataatatttatatgaaGTTAGGAAGCAAAAAAGCAAACTATAGTTAAATACCATATATTTGATCAgtc +aagaaaaaacaacCAAAACCAAGATTTATGAGAAAGATGTGCAAAGACACGTgataaaaattcaaattggGATCAATCGT +TTTTTATGAATCACATTTGATaccatatttttcatattttttattttttttatttcttgcatatatatattttttttttt +atttataataaccttcttttccttcaaaaataaattatttcaaaaattttctttatcattttaaATCACTAAATTATTCC +AATACTATATACTATATCACAAATTAGAACTGttcattaaaatttaaCCAATACCTAATAtgtcaaagaaaatactaTCT +TTCCAATTACCTAAAgtaaatagaaataaagataacAATATAGCTGTTACTGGATTTCCTTATATGGATAGAACCACCAA +AAAACATACAAGTTATGAAACATACAATGTTATTGCAGTTAATTGCGATGTTATTGTATCTACCGTAATACTGAAAAAga +gtaataaaaataataatagaaaagaagaaccAACAAGACCTAGGAATAAATTCATGATAGCAAGAagttttattaattgt +ttATTAAGATACAAAGAGACAGCGATAAATAGCCAAACGGTATCAAAGATAGTTTCAGATATCTGGAGAAACTCAGGTCA +GAACTTTGTGAgttattttgattatctTGCGATATTCGACAATAATCTTCACCAGCagatatttgataatgattttttaa +gattcaaaacaaaaagaaccATAAATAAGAAGCACTTAAATATGAAAGGTACCTTTGTCTATGAAGTTCCTGAGAAATCA +TACCTTGCAGAGCAAATTGAACCTCAAACcatattaatgaaatctAGAAAAGAGTATCTTGATTTATGTGCAAGCTCACT +ATTAGGAAAGGAGAACACAATATTAGAATCGACATTGAAGATTAATGCTCATTGTGATACAAAACTGGTCGCACTACCAT +TTGACATATTAAAAAACTCAAAAAAAACTCAATATGGAAAACctagaaaatcaaaaaaattagatcTTAAACAGCCGATG +TTCTCAAAGTTTTCTATACTCTCCAGTAAAGTGGGAAagcaaaaaatttaatcCTAATTTGACATCTACTATGACGCATA +AAGCAactatataataaataatacttaaataaattagaatttttttggCTGTACACATTTTTTGCGCATAAACTTTATTG +TCCTGCGTGCTAATTATTTCccaaaaataatcattacAATTTGAACTCAAAgctaaaaaaatgatagaaaagactataaa +aaaattataaaaaaattataaaaaaaaaaaatagatatatatatatctatactgttatagatatatatatatctattttt +tttttatatatatacatgtatttatttaatgatgatgaacaGCATCCAAGGCTTTTGGATGGTGAATAATAGCATCCAAA +GCTTTTCCAACAGCTTCAAAATCAGTCATTAAACTAACATGACCACAATCACTAGGAACTTTAGCTACAGTTTTAAAACC +TTCATAATTTTCAGGCATCAACCATTTTGAATGAATAACGCCATCACCAGGTCCATAAAAGTAATTATCGTATTGACCAT +CTTTAATGTCCTTCTCACCATCGACCAACACACCTCGCACTGAAGGGACAGTATTTCCGTAAACTAAAGCTAATGGTGGA +TACttatctttcaattctGGATTGTAACTTAACTCGTCGAGAAATTTTTTAGTTCTTTGTAATGTACGCGAAAGGTATTC +ATAAGATTGCATGCCTTTGTGTGAGTTAGATACTAATGGAGACAAGTTATATTTGACCCAAGTATCAGGGTTGAACAAAT +CCATATGTATGAATTTTTCCGTGTTTGtatcaaagaaaacatTTCCATGTCTAGGCAAGAACACAAAGCTTGAACGCATC +AGGAAATTCGCTTCAGCTGTCAAAACATTCTTACTGAGTAATACAGAGTCCCCGAATCTTATAGGACCAAGAATGTTAAG +ACATTCACTAGGGGTCCCCACATATAAAATACCATTGAAAAGCGTTGGATCTTTGTTCATTGCATGATGTGCAATTAATC +CCCCCATTGAATGTGCCACAACCAACGCtcctttgttttttttgccCTTTTCACCGTTcgtattattattactgttatta +ttattattattattattattattattattgttgttgttgttgttgcatGGTAGCTCTTTAAGGAACGCCTCAAGCTTTGC +TGAATTGATATCACAAGACAATCTCCAATCATACCCAAATTCATGTACTCGTACCCCGGGCACGGCCGCCATCTTCTTGA +TGAGTTTCTTCGAGATATCTATAGGCCCTATATTGGTCAACATCCCATCAGGGTATATCTTTTTAGGCATGTCATACTCA +GCTTGGTCATCCGGAGGCACCACCAAATCCACCTTTCTCAAGTTGAGCCCCACCTTCATCGGGATCCAAAGCATTCGCCC +TGTTTCTGTGTCTCTTAAAACAGACCCCCGATAGCCTCCTAGCACCACTATGTCGCCCTCTAGCTTTTCAAATACCGTTT +CCGTGTAATATTCATATGATTTTTCTCCAGTTTTTTCGCTAATGAACGCCGGTGTAAGTGCCTTTTTCACCGCACGAAGT +CTCGAGTTGTCTGCTCCCTTGAGCTCCCTATAAGCATAAGCCGCCTCAAGTGTACTCATAGAATCCTGTCGCTTCAATTT +CTGTCGAAGCTGCTTGTCTGTAATGTTCTCGGGCACCGGAAGTGGATAACTGCTGTCATcgttttgaaaaaatctcAATT +TTGGCATGCTAATCCTTGATGGTAGAATGTCTGTAAGTGTATCTGAAAATGGTAAAGTTAATGAAAATACCCGTCGATTC +TCAGCAGCTGTCTTATTCTTAGGCGAGGAGCAAATATTGGTGTTTccattattactactattaCTGCTACTTCTACTGCT +GCTTCTGCTACTAGTAGTATTGCTATGTgtagtattttttttcccaaaagaaataaacGATGATTTCTGTTTTATATAAT +TAAGTGAGCTTTCTACCAGCATAAGACTGTTGTGTCTCGATGGTCGAGTGGCCGtgaatttcttcttttctagTTCCATA +GAATCAATGATATTTCTTAACTTGTTACCTGCAATTATCGCGGCTTCCTCACCCtgatttttaattttagcAATAGTTCC +CACATTTACTGCTTtctgttgctgctgctgtaCTGCCACTGCTTCTCTTGCATCGCCTTTCAGGATTTGTTTctctcttt +cttctttgttttcattttcattttcatttccattttcttcctcttcttctactACTCCCTGGTCATTGTCATATACGTCA +TTAAAAGAGGAACCACCAATCAATGTTGAGGCTACGCTTCGCGATATTTGTCTTGTTAGTTCGTAGTCATTATGGTCGAT +GTATGTATTTGTATGGTTCTCATCATTATGAATATCATCGCCAAAATAATAGGATTGCTCAGCTGTTGTAACATCATTTT +GAATGTTGTCAAGCTTATTCGGCTTAGTCTCAAGTTTTTGCCCTATATTTGGTGATTTGTCAAGCTTTGATTCTTTTGTA +TCCTTTTTCAGAGTGGGTACTGTATCAAGGGGAGTCgccatttttttatttaatctatGGGATGTTTTATGCTACTAATA +AACGCTAGAGTGAACAATATGGTATCAGCAGAAGAACACTTAAATACAAATCAAActtattttcaactttttgaaGCAGT +TTAATCTCAGAAACAGCAAATGATATTCTTCATCTAGAATTCCGTCAATGCATACCAATATCCAGGATGAAGCCAGTGTA +CTCATATGCCGCATCCGACTATTGGTGCCAACatcataaaaatatcaattagGGATTGGAAATATATTTGCAGCTCCTTC +ATTTTGATGACAAGTGCATGACTCAGTGAATTGATGATCAATTTAgatatttttgtatatttctGTAAAGATAGTTTCTA +CGAGGATTAAATGTTAGTAGGTTCATTATTTAGATAAAATCTACAGTATCTTGTCTTCTTAATCTCAAAAATATTCCTAA +GAAGAGTAGTACACCACCATTGTAGCTTTTTATTCTTGCCATCCAATGTTTTACCTAGCAGAGTTTCTTGCACAAATGAC +ATGTTTTAtgagtttttttttttatatctttCCCCCCACTTATTTCAGTGCCTTCTTTCTTCAAGCAttaatctttttct +ctatatcacaatttccaattttttcacAACCTTTTAACACAGCTTCGGATAAGTTATTAACAGCATTATAAAATTCGATA +GCACTATCAATATATTGCTGTTGGAAATTGAtacatgttgaaaattgcCCTTCTTGGCAAATAAGACGTCATATCTTGCC +ATACGTTTCATAACAATAAACATTATTCAGCACAAATTAGctttttctgaattttttataaattatttacttAATCGTTT +GTCAAAAGTATACGTatccaaaaaataaataagttCAACGAAGTgcgtttttttttatagaATTTCATGAGCCAGATAAT +TTCAGGGCACATAGTATATACtgtgaagaaaaaatgggGTGTCGGATGATTTGGCTCGGATCATATGCCCCAGTATAGac +aatatttttactttcatcaataatgaCAGAGTCATGCTGTTTAGATGAATTCAATAGATTTAAACTATGTTATATTTAAT +CAGAGTTGTTATTAAACCAAGTTATCATGTCCATAAGCCGATTATcggaatttgaaattatttccGTTTAGTTAATCATg +aaatttgatttaatttGCTTAAGTCATTATTCATGgatgaaatttttgttttattcaaatattaagaAAGTAGTAAATAA +TTGTTgttctctttcttcttttatcttCTAGCTTCTTATAT diff --git a/results/2026-10-30_saccharomycopsis/make_records.py b/results/2026-10-30_saccharomycopsis/make_records.py new file mode 100644 index 00000000..e597b390 --- /dev/null +++ b/results/2026-10-30_saccharomycopsis/make_records.py @@ -0,0 +1,90 @@ +"""Draft MATss proposals for S. schoenii (GDD12, GCA_054953955.1) from our own chromosome IV gene models. + +Models: results/2026-10-29_ss_records (StringTie + TransDecoder on PRJNA1364152 RNA-seq; the authors' GTF is not public). +Each CDS is re-extracted from the window fasta and checked (ATG start, no internal stop, stop at the end, length) before writing. +Run from the repo root: python3 results/2026-10-30_saccharomycopsis/make_records.py [outdir] +""" +import sys, yaml +from Bio.Seq import Seq + +HERE = "results/2026-10-30_saccharomycopsis/" +OUT = sys.argv[1] if len(sys.argv) > 1 else HERE +WIN_START = 463000 +seq = "".join(l.strip() for l in open(HERE + "chrIV_463k-542k.fa") if not l.startswith(">")) + +def cds(exons, strand): + s = "".join(seq[a - WIN_START:b - WIN_START + 1] for a, b in sorted(exons)) + s = s if strand == "+" else str(Seq(s).reverse_complement()) + return s + +def protein(exons, strand): + return str(Seq(cds(exons, strand)).translate(table=12)) # CUG = Ser (NCBI table 12) + +# name: (role, strand, exons ascending, expected aa, note) +LEFT = { + "MATalpha1": ("core_MAT", "-", [(471020, 471595)], 191, "alpha box (MATalpha_HMGbox 54 bits), SS.1982 ORF p4"), + "MATalpha2": ("core_MAT", "+", [(471961, 472227), (472445, 472489)], 103, "homeodomain (57 bits), partial ORF; 217 bp intron, 198 reads"), + "sla2": ("flanking_conserved", "-", [(472708, 473478)], 256, "diverged SLA2 (I_LWEQ 186 bits); a second piece 473,680-474,606 (309 aa) is not recorded"), +} +RIGHT = { + "sla2": ("flanking_conserved", "+", [(533981, 535828)], 615, "SLA2 anchor (I_LWEQ 183 bits), SS.2002.2 ORF p1; mirrored copy 529,992-532,490"), + "MATa1": ("core_MAT", "-", [(536046, 536090), (536296, 536634), (536693, 536944)], 211, "homeodomain (54 bits); introns 205 bp (221 reads) and 58 bp (218 reads)"), +} +for nm, genes in (("left", LEFT), ("right", RIGHT)): + for g, (role, strand, ex, aa, _) in genes.items(): + p = protein(ex, strand) + print(nm, g, len(p) - (1 if p.endswith("*") else 0), "aa expected", aa, "start", p[0], "stop", p.endswith("*"), "internal stops", p[:-1].count("*")) + assert p.endswith("*") and p[:-1].count("*") == 0 and len(p) - 1 == aa, (g, len(p)) + +CIT = [{"pmid": "41849659", "doi": "10.1093/g3journal/jkag067"}, {"pmid": "38492364", "doi": "10.1016/j.micres.2024.127691"}] +TAX = {"taxid": 44080, "lineage": "k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Ascoideales;f__Saccharomycopsidaceae;g__Saccharomycopsis;s__Saccharomycopsis_schoenii", + "lineage_resolved_date": "2026-10-30"} +ORG = {"species": "Saccharomycopsis schoenii", "strain": {"name": "GDD12", "known": True, "culture_collection_ids": [], "differs_from_sequenced": False}} + +def genes_of(spec): + out = [] + for i, (name, (role, strand, ex, aa, note)) in enumerate(sorted(spec.items(), key=lambda kv: min(a for a, b in kv[1][2]))): + exons = [{"start": a, "end": b} for a, b in (sorted(ex) if strand == "+" else sorted(ex, reverse=True))] + out.append({"gene_index": i, "name": name, "role": role, "present": True, "locus_tag": None, "segment_index": 0, + "start": min(a for a, b in ex), "end": max(b for a, b in ex), "strand": strand, "order_in_locus": i, + "transl_table": 12, "exons": exons}) + return out + +def record(rid, idiomorph, spec, orient, note, span): + genes = genes_of(spec) + lo = min(g["start"] for g in genes); hi = max(g["end"] for g in genes) + loc = {"coordinate_provenance": "curator_derived", "excluded_from_coordinate_benchmark": False, + "core": {"completeness": "partial", "reference_orientation": orient, "definition_note": note, + "segments": [{"segment_index": 0, "sequence_source": {"type": "insdc_nucleotide", "accession": "CM143129.1", "seq_region": "CM143129.1"}, + "start": lo, "end": hi, "contig_edge_distance": None, "sequence_checksum": None}]}, + "extended_flank": [], "assembly_accession": "GCA_054953955.1", "assembly_accession_basis": "strain_match_verified", + "assembly_location": {"contig": "CM143129.1", "start": lo, "end": hi, "identity": 100.0}} + return {"record_id": rid, "record_version": 1, "taxonomy": TAX, "organism": ORG, + "mating_type": {"locus_name": "MATss", "idiomorphs": [idiomorph], "system": "homothallic"}, + "locus": loc, "genes": genes, + "evidence": {"locus_existence": {"tier": 2, "experimental_method": "published description of the SLA2-MAT cassettes and RNA-seq of the active chromosome IV locus (Kriti et al. 2026)", "citations": CIT}, + "boundaries": {"tier": 3, "experimental_method": "OUR OWN gene models from the public RNA-seq (PRJNA1364152; StringTie, TransDecoder, phmmer/Pfam) because the authors' GTF is not public; coordinates are ours, the locus span is the paper's", "citations": CIT}, + "idiomorph_assignment": {"tier": 3, "experimental_method": "the paper's assignment of the sub-cluster (alpha-dominant left, palindromic a right); gene names follow it by position; phmmer ranks both homeodomain ORFs closest to MATa1-type references, so MATalpha2 is a hypothesis", "citations": CIT}}, + "validation": {"status": "needs_review", "rejection_reason": None, "accession_resolved": False, "accession_resolved_date": None, + "accession_resolved_version": None, "sequence_match": {"status": "pass", "per_gene": [], "notes": ""}, "taxonomy_current": None}, + "curation": {"proposed_by": "claude-code-curation-agent", "proposal_dedupe_key": f"41849659|44080|MATss|{idiomorph}", "reviewed_by": None, "reviewed_date": None}, + "model_provenance": None} + +NOTE_L = ("Left (alpha-dominant) sub-cluster of the active MAT locus on chromosome IV of S. schoenii GDD12 (CM143129.1; the paper's span 470,610-475,000, " + "Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC (Dost et al. 2024, PMID 38492364); one of several SLA2-MAT cassettes (one per chromosome plus a palindromic one on chromosome IV). " + "GENE MODELS ARE OURS (tier 3): the BRAKER2 GTF the paper cites is not on its GitHub or Zenodo, so the models were rebuilt from the three public paired RNA-seq runs " + "(PRJNA1364152; hisat2, StringTie, TransDecoder, phmmer against the curated MAT proteins, Pfam-A); see results/2026-10-29_ss_records/README.md. The library is not stranded; " + "strand is the ORF's. MATalpha1 is single-exon, minus strand, 191 aa. The homeodomain ORF (named MATalpha2 after the paper) is partial (103 aa, two exons with a 217 bp intron of 198 reads that " + "joins it toward SLA2, the paper's MAT-SLA2 splice junction); phmmer ranks it closest to MATa1-type references (50 bits to C. albicans MTLA1), so the name is a hypothesis. " + "SLA2 here is a diverged copy: 256 aa (472,708-473,478, minus), 5-prime partial (the ORF has no start codon); a second SLA2 piece (473,680-474,606, 309 aa) is not recorded. An HMG-like ORF at 470,349-470,951 (200 aa, 44 bits to " + "S. cerevisiae MATA2, no Pfam hit) is not assigned to a gene. GENETIC CODE: NCBI table 12 (CUG = Ser) for the species; proteins are translated with it.") +NOTE_R = ("Right (palindromic) cassette of the active MAT locus on chromosome IV of S. schoenii GDD12 (CM143129.1; the paper's span 528,044-537,777; an SLA2 anchor between mirrored MAT arrays, " + "Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC (Dost et al. 2024, PMID 38492364). The record covers the arm that RNA-seq resolves: SLA2 533,981-535,828 (plus, 615 aa, 5-prime partial: no start codon in the ORF) and the homeodomain gene " + "named MATa1 after the paper at 536,046-536,944 (minus, 211 aa; introns of 205 bp, 221 reads, and 58 bp, 218 reads, the conserved 58 bp junction the paper reports at 536,635). The mirrored arm " + "(SLA2 529,992-532,490; homeodomain ORF 529,258-529,749, 163 aa) is not recorded: short reads cannot separate the two inverted copies, and the paper's mirrored junction at 529,128 is not seen. No HMG-box " + "(MATa2) ORF passed the filters. GENE MODELS ARE OURS (tier 3), see results/2026-10-29_ss_records/README.md. The library is not stranded. GENETIC CODE: NCBI table 12 (CUG = Ser).") +ra = record("44080_gdd12_MATss_MATalpha", "MATalpha", LEFT, "MATalpha1->MATalpha2->sla2", NOTE_L, None) +rb = record("44080_gdd12_MATss_MATa", "MATa", RIGHT, "sla2->MATa1", NOTE_R, None) +for r in (ra, rb): + open(f"{OUT}{r['record_id']}.yaml", "w").write(yaml.safe_dump(r, sort_keys=False)) + print(r["record_id"], [(g["name"], g["start"], g["end"], g["strand"], len(g["exons"])) for g in r["genes"]]) diff --git a/results/2026-10-30_saccharomycopsis/validate.slurm b/results/2026-10-30_saccharomycopsis/validate.slurm new file mode 100644 index 00000000..14576c28 --- /dev/null +++ b/results/2026-10-30_saccharomycopsis/validate.slurm @@ -0,0 +1,11 @@ +#!/usr/bin/bash +#SBATCH -N 1 -c 1 --mem 2gb --time 00:30:00 -J ss-validate +set -euo pipefail +R=/bigdata/stajichlab/jstajich/projects/MATPredict +T=$R/results/2026-10-30_saccharomycopsis/tree +export PATH="$R/.pixi/envs/default/bin:$PATH" +cd $T +for r in 44080_gdd12_MATss_MATalpha 44080_gdd12_MATss_MATa; do + PYTHONPATH="$T/src" MATPREDICT_DB_ROOT="$T/db" python -m MATPredict curate-db validate --phylum Ascomycota --record-id $r > $T/$r.validate.json + grep -n "taxonomy_current\|taxonomy_error\|sequence_match\|status" -A1 $T/$r.validate.json | head -12 +done diff --git a/tests/detect/test_fallback_searchable.py b/tests/detect/test_fallback_searchable.py index 9a2250df..7f9e3558 100644 --- a/tests/detect/test_fallback_searchable.py +++ b/tests/detect/test_fallback_searchable.py @@ -53,7 +53,7 @@ def test_shipped_roster_flags_the_scope_only_families(): Yarrowia `MATyl` and Geotrichum `MATdc` (curator 2026-10-17), Komagataella `MATkp` and the Ogataea polymorpha-complex `MATog` (curator 2026-10-17/18).""" flagged = {f.key.locus_name for f in load_all_families(DB) if not f.fallback_searchable} - assert flagged == {"redPR", "redHD", "rustHD", "wallMAT", "MATyl", "MATdc", "MATkp", "MATog", "MATpk"} + assert flagged == {"redPR", "redHD", "rustHD", "wallMAT", "MATyl", "MATdc", "MATkp", "MATog", "MATpk", "MATss"} def test_pr_scope_includes_boletales(): diff --git a/tests/detect/test_matss_family.py b/tests/detect/test_matss_family.py new file mode 100644 index 00000000..add3136f --- /dev/null +++ b/tests/detect/test_matss_family.py @@ -0,0 +1,41 @@ +"""The MATss (Saccharomycopsis) family and its two S. schoenii candidate records (curator, 2026-10-30).""" +from pathlib import Path + +import yaml + +from MATPredict.detect.family_registry import FamilyKey, load_all_families, route + +DB = Path(__file__).resolve().parents[2] / "db" + + +def _family(): + return next(f for f in load_all_families(DB) if f.key == FamilyKey("Ascomycota", "MATss")) + + +def test_matss_declaration(): + fam = _family() + assert list(fam.taxonomic_scope) == [4943] + assert fam.fallback_searchable is False and fam.cassette_report is False and fam.two_idiomorphs_report is False + genes = {g["name"]: g for g in fam.genes} + assert genes["MATalpha1"]["gene_class"] == "alpha_box" + assert genes["sla2"]["gene_class"] == "sla2_homolog" + assert set(genes) == {"MATa1", "MATa2", "MATalpha1", "MATalpha2", "sla2", "dic1"} + + +def test_matss_candidates_are_tier_3_models_with_table_12(): + for rid in ("44080_gdd12_MATss_MATalpha", "44080_gdd12_MATss_MATa"): + rec = yaml.safe_load((DB / "candidates" / "Ascomycota" / rid / "metadata.yaml").read_text()) + assert rec["mating_type"]["locus_name"] == "MATss" and rec["mating_type"]["system"] == "homothallic" + assert rec["evidence"]["boundaries"]["tier"] == 3 + assert all(g["transl_table"] == 12 for g in rec["genes"]) + assert rec["validation"]["status"] == "needs_review" + + +def test_matss_routes_the_genus_only(): + fams = load_all_families(DB) + base = [131567, 2759, 33154, 4751, 451864, 4890, 716545, 147537, 4891, 2926619, 34366, 4943] + d = route(44080, fams, lineage_taxids_resolver=lambda t: base + [44080], phylum_name_resolver=lambda t: "Ascomycota") + assert d.routing_mode == "lineage" and "MATss" in {f.key.locus_name for f in d.families} + d = route(4909, fams, lineage_taxids_resolver=lambda t: [131567, 2759, 33154, 4751, 451864, 4890, 716545, 147537, 4909], + phylum_name_resolver=lambda t: "Ascomycota") + assert "MATss" not in {f.key.locus_name for f in d.families} From f896ce13107fab545e62f4ef938ea88ed41165ac Mon Sep 17 00:00:00 2001 From: Jason Stajich Date: Fri, 30 Oct 2026 06:20:27 -0700 Subject: [PATCH 2/5] db: MATpf and MATbz families and three more tier-3 candidate records (P. fabianii MATa and MATalpha, B. californica PB4207); annotation work for both genera Co-Authored-By: Claude Sonnet 5.5 --- db/Ascomycota/order.yml | 37 + .../36022_joy008_MATpf_MATa/metadata.yaml | 169 ++ .../metadata.yaml | 155 ++ .../metadata.yaml | 177 ++ results/2026-10-30_barnettozyma/.gitignore | 2 + .../36038_pb4207_MATbz_MATa-MATalpha.yaml | 170 ++ .../MATbz_family_proposal.yml | 18 + results/2026-10-30_barnettozyma/README.md | 38 + .../2026-10-30_barnettozyma/a2_benchmark.py | 27 + results/2026-10-30_barnettozyma/a2_refs.faa | 16 + ..._003123585.1_QBLI01000035.1_1-51373.a2.gff | 1 + .../GCA_003123585.1_QBLI01000035.1_1-51373.fa | 736 ++++++++ ...03123585.1_QBLI01000035.1_1-51373.lift.gff | 22 + ...3585.1_QBLI01000035.1_1-51373.miniprot.gff | 84 + .../data/GCA_003123585.1_orf_scan.tsv | 8 + ...3885.1_QBLJ01000026.1_100869-167498.a2.gff | 1 + ...03123885.1_QBLJ01000026.1_100869-167498.fa | 735 ++++++++ ...85.1_QBLJ01000026.1_100869-167498.lift.gff | 22 + ..._QBLJ01000026.1_100869-167498.miniprot.gff | 82 + .../data/GCA_003123885.1_orf_scan.tsv | 9 + ...06655.3_PPLX03000005.1_95524-162156.a2.gff | 1 + ...003706655.3_PPLX03000005.1_95524-162156.fa | 954 ++++++++++ ...655.3_PPLX03000005.1_95524-162156.lift.gff | 22 + ...3_PPLX03000005.1_95524-162156.miniprot.gff | 84 + .../data/GCA_003706655.3_orf_scan.tsv | 9 + ...5.1_JAMAIE010000005.1_589225-655853.a2.gff | 1 + ...27795.1_JAMAIE010000005.1_589225-655853.fa | 954 ++++++++++ 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8 + .../scan_one_intron.py | 75 + .../scan_two_introns.py | 58 + results/2026-10-30_petasospora/.gitignore | 4 + .../36022_joy008_MATpf_MATa.yaml | 158 ++ .../36022_tbrc-4498_MATpf_MATalpha.yaml | 144 ++ results/2026-10-30_petasospora/README.md | 41 + .../2026-10-30_petasospora/build_models.py | 52 + .../2026-10-30_petasospora/classify_aug.py | 53 + ...9195.1_JCM_3601_assembly_v001.miniprot.gff | 86 + .../GCA_001983305.1_ASM198330v1.miniprot.gff | 72 + .../GCA_022641835.1_ASM2264183v1.miniprot.gff | 87 + .../GCA_050575385.1_ASM5057538v1.miniprot.gff | 72 + .../2026-10-30_petasospora/data/all_refs.faa | 137 ++ .../data/aug_candida_guilliermondii.gff3 | 1557 +++++++++++++++ .../data/aug_classified.tsv | 171 ++ .../data/aug_kluyveromyces_lactis.gff3 | 1634 ++++++++++++++++ .../data/aug_pichia_stipitis.gff3 | 1508 +++++++++++++++ .../aug_saccharomyces_cerevisiae_S288C.gff3 | 1666 +++++++++++++++++ .../2026-10-30_petasospora/data/ena_runs.tsv | 26 + .../data/extra_refs.faa | 11 + 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Optional so that truncated copies are not demoted. - {name: sla2, role: flanking_conserved, gene_class: sla2_homolog, optional: true} - {name: dic1, role: flanking_conserved, optional: true} + # NEW FAMILY, curator decision 2026-10-30 (candidate records for the SLA2-type genera): Petasospora (Cyberlindnera) fabianii has its own + # scope-only family, named after the species as MATpk. SCOPE = THE SPECIES (taxid 36022), the same restraint as MATpk and MATog: one + # species is curated. HETEROTHALLIC with one idiomorph per genome in the four assemblies checked; no second cassette found. Gene models are + # OUR OWN predictions (tier 3: AUGUSTUS and miniprot, no RNA-seq exists for the species); see results/2026-10-30_petasospora/README.md. + - locus_name: "MATpf" + vocabulary_type: "enum" + idiomorph_values: ["MATa", "MATalpha"] + idiomorph_class: {MATalpha: unassigned, MATa: unassigned} + taxonomic_scope: [36022] + fallback_subphylum: 147537 # Saccharomycotina + fallback_searchable: false + flank_carried_window_bp: 20000 + genes: + - {name: MATa1, role: core_MAT, present_in_idiomorphs: ["MATa"]} # homeodomain (Pfam Homeodomain 64 bits) + - {name: MATa2, role: core_MAT, present_in_idiomorphs: ["MATa"]} # HMG-box; no Pfam hit above GA, MATa2 Saccharomycotina profile 76.8 bits + - {name: MATalpha1, role: core_MAT, gene_class: alpha_box, present_in_idiomorphs: ["MATalpha"]} # PF04769 36 bits + - {name: MATalpha2, role: core_MAT, present_in_idiomorphs: ["MATalpha"], optional: true} # none found in either alpha allele + - {name: dic1, role: flanking_conserved, optional: true} + - {name: sla2, role: flanking_conserved, gene_class: sla2_homolog, optional: true} + # NEW FAMILY, curator decision 2026-10-30: Barnettozyma (Phaffomycetales) has its own scope-only family, named after the genus; scope = the genus + # (taxid 599802). One record, B. californica PB4207, from OUR OWN tier-3 models (no RNA-seq for the genus; the paper, Hession et al. 2025, + # PMID 41359681, gives no coordinates); see results/2026-10-30_barnettozyma/README.md. MATalpha2 is not modelled, hence optional. + - locus_name: "MATbz" + vocabulary_type: "enum" + idiomorph_values: ["MATa", "MATalpha"] + idiomorph_class: {MATalpha: unassigned, MATa: unassigned} + taxonomic_scope: [599802] + fallback_subphylum: 147537 + fallback_searchable: false + flank_carried_window_bp: 20000 + genes: + - {name: MATa1, role: core_MAT, present_in_idiomorphs: ["MATa"]} + - {name: MATa2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATa"]} # divergent HMG-like; no Pfam HMG_box hit + - {name: MATalpha1, role: core_MAT, gene_class: alpha_box, present_in_idiomorphs: ["MATalpha"]} + - {name: MATalpha2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATalpha"]} # not modelled in PB4207 + - {name: sla2, role: flanking_conserved, gene_class: sla2_homolog, optional: true} + - {name: dic1, role: flanking_conserved, optional: true} diff --git a/db/candidates/Ascomycota/36022_joy008_MATpf_MATa/metadata.yaml b/db/candidates/Ascomycota/36022_joy008_MATpf_MATa/metadata.yaml new file mode 100644 index 00000000..92f9e5be --- /dev/null +++ b/db/candidates/Ascomycota/36022_joy008_MATpf_MATa/metadata.yaml @@ -0,0 +1,169 @@ +record_id: 36022_joy008_MATpf_MATa +record_version: 1 +taxonomy: + taxid: 36022 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Phaffomycetales;f__Wickerhamomycetaceae;g__Petasospora;s__Petasospora_fabianii + lineage_resolved_date: '2026-10-30' +organism: + species: Petasospora fabianii + strain: + name: JOY008 + known: true + culture_collection_ids: + - JOY008 + differs_from_sequenced: false +mating_type: + locus_name: MATpf + idiomorphs: + - MATa + system: heterothallic +locus: + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + assembly_accession: GCA_022641835.1 + assembly_accession_basis: sequence_link + assembly_location: + contig: CP048715.1 + start: 628449 + end: 644871 + identity: 100.0 + core: + completeness: complete + reference_orientation: DIC1->MATa2->MATa1->(six conserved genes)->SLA2 + definition_note: 'MATa idiomorph of P. fabianii isolate JOY008 (ecotype Missouri, + St. Louis College of Pharmacy), complete genome GCA_022641835.1, CP048715.1 + (BioSample SAMN14051719). The type strain JCM 3601 (GCA_001599195.1, BCGI01000001.1:641,150-657,563) + carries the same MATa locus with 99-100% identical proteins (MATa1 and MATa2 + one substitution each). The v0.7.0 MATsc call (CP048715.1:630,345-685,843, 55 + kb) is wider than the locus: the genes are DIC1 628,452-629,375 to SLA2 641,746-644,871. + GENE MODELS ARE OURS (tier 3, manual annotation; curator decision 2026-10-30). + No RNA-seq exists for the species in ENA/SRA (checked 2026-10-30), so the models + are AUGUSTUS ab initio predictions (species models S. cerevisiae S288C, C. guilliermondii, + P. stipitis, K. lactis) chosen where several agree and where homology supports + them; MATa2 is also supported by the Saccharomycotina MATa2 profile (76.8 bits, + above the 52.4 bits of P. kudriavzevii). Coordinates include the stop codon. + Intron signals: MATa2 72 bp GTAGGT..TACTAAC..TAG (strong); MATa1 97 bp GTACCA..AAAG + (weak donor; an upstream exon is required because the homeodomain open frame + has no start codon, but the splice sites are unconfirmed). The MATalpha1 model + is a single exon of 305 aa; the depositor''s ONH67106 (254 aa, strain 65) is + the same C-terminal 185 aa plus a 69 aa exon joined across a 1,263 bp intron + (GTCCTT..CCCCAG) that has no support here: the N-terminus is unresolved. GENE + NAMES by homology and position: MATa1 is the homeodomain protein (Pfam Homeodomain + 64 bits, 66 bits to Komagataella MATa1), MATa2 the HMG-box protein (divergent; + no Pfam HMG_box hit above GA), MATalpha1 the alpha-box protein (PF04769 36 bits, + 407 bits to ONH67106). No MATalpha2 homeodomain gene was found in either alpha + allele (only a 57 aa Homeobox_KN fragment at 12-18 bits about 1.2 kb upstream + of MATalpha1). LAYOUT: DIC1 (minus) - 0.5 kb (MATa) or 5.0 kb (MATalpha) - MAT + genes - six conserved non-MAT ORFs (409, 173, 589, 219, 465, 622 aa; identical + in both alleles, not recorded) - SLA2 (plus); SLA2 is therefore about 9.5 kb + from the MAT genes. The alpha allele also carries an 800 aa MutS_III-domain + ORF between DIC1 and MATalpha1 that has no counterpart in the MATa allele (not + recorded). No second MAT cassette: miniprot of the MAT proteins finds no other + MAT gene in any of the four assemblies, so the species looks heterothallic with + one idiomorph per genome. GENETIC CODE: standard (table 1). Details: results/2026-10-30_petasospora/README.md.' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: CP048715.1 + seq_region: CP048715.1 + start: 628449 + end: 644871 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] +genes: +- gene_index: 0 + name: dic1 + protein_accession: null + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 628449 + end: 629375 + strand: '-' + order_in_locus: 0 + exons: + - start: 628449 + end: 629375 +- gene_index: 1 + name: MATa2 + protein_accession: null + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 629850 + end: 630863 + strand: '-' + order_in_locus: 1 + exons: + - start: 630492 + end: 630863 + - start: 629850 + end: 630419 +- gene_index: 2 + name: MATa1 + protein_accession: null + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 631437 + end: 632136 + strand: + + order_in_locus: 2 + exons: + - start: 631437 + end: 631737 + - start: 631835 + end: 632136 +- gene_index: 3 + name: sla2 + protein_accession: null + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 641746 + end: 644871 + strand: + + order_in_locus: 3 + exons: + - start: 641746 + end: 644871 +evidence: + locus_existence: + tier: 3 + experimental_method: 'manual annotation: homology (curated Saccharomycotina MAT + proteins, Pfam, MATa2 profile) and AUGUSTUS ab initio models on the deposited + assembly; no RNA-seq' + citations: &id001 [] + boundaries: + tier: 3 + experimental_method: AUGUSTUS ab initio gene models checked against homology; + DIC1 and SLA2 are the flanking anchors, MAT genes lie between them + citations: *id001 + idiomorph_assignment: + tier: 3 + experimental_method: 'gene content: MATa1 (homeodomain) with MATa2 (HMG-box) versus + MATalpha1 (alpha box), at the same DIC1-SLA2 position in different strains' + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: '' + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: manual|36022|MATpf|MATa + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/db/candidates/Ascomycota/36022_tbrc-4498_MATpf_MATalpha/metadata.yaml b/db/candidates/Ascomycota/36022_tbrc-4498_MATpf_MATalpha/metadata.yaml new file mode 100644 index 00000000..ca9014e5 --- /dev/null +++ b/db/candidates/Ascomycota/36022_tbrc-4498_MATpf_MATalpha/metadata.yaml @@ -0,0 +1,155 @@ +record_id: 36022_tbrc-4498_MATpf_MATalpha +record_version: 1 +taxonomy: + taxid: 36022 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Phaffomycetales;f__Wickerhamomycetaceae;g__Petasospora;s__Petasospora_fabianii + lineage_resolved_date: '2026-10-30' +organism: + species: Petasospora fabianii + strain: + name: TBRC 4498 + known: true + culture_collection_ids: + - TBRC 4498 + differs_from_sequenced: false +mating_type: + locus_name: MATpf + idiomorphs: + - MATalpha + system: heterothallic +locus: + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + assembly_accession: GCA_050575385.1 + assembly_accession_basis: sequence_link + assembly_location: + contig: CM115473.1 + start: 631646 + end: 651160 + identity: 100.0 + core: + completeness: complete + reference_orientation: DIC1->MATalpha1->(six conserved genes)->SLA2 + definition_note: 'MATalpha idiomorph of P. fabianii TBRC 4498 (xylitol-producing + strain; PMID 40558965), chromosome-level GCA_050575385.1, CM115473.1. The draft + genome of strain 65 from fermented masau fruit (GCA_001983305.1, MPUK01000005.1:611,410-630,920; + PMID 28385833) carries the same locus with an identical MATalpha1 protein and + holds the depositor''s ONH67106 (''Mating-type-like protein ALPHA1'', 254 aa, + MPUK01000005.1:616,190-616,396 + 617,660-618,217), which our model replaces. + GENE MODELS ARE OURS (tier 3, manual annotation; curator decision 2026-10-30). + No RNA-seq exists for the species in ENA/SRA (checked 2026-10-30), so the models + are AUGUSTUS ab initio predictions (species models S. cerevisiae S288C, C. guilliermondii, + P. stipitis, K. lactis) chosen where several agree and where homology supports + them; MATa2 is also supported by the Saccharomycotina MATa2 profile (76.8 bits, + above the 52.4 bits of P. kudriavzevii). Coordinates include the stop codon. + Intron signals: MATa2 72 bp GTAGGT..TACTAAC..TAG (strong); MATa1 97 bp GTACCA..AAAG + (weak donor; an upstream exon is required because the homeodomain open frame + has no start codon, but the splice sites are unconfirmed). The MATalpha1 model + is a single exon of 305 aa; the depositor''s ONH67106 (254 aa, strain 65) is + the same C-terminal 185 aa plus a 69 aa exon joined across a 1,263 bp intron + (GTCCTT..CCCCAG) that has no support here: the N-terminus is unresolved. GENE + NAMES by homology and position: MATa1 is the homeodomain protein (Pfam Homeodomain + 64 bits, 66 bits to Komagataella MATa1), MATa2 the HMG-box protein (divergent; + no Pfam HMG_box hit above GA), MATalpha1 the alpha-box protein (PF04769 36 bits, + 407 bits to ONH67106). No MATalpha2 homeodomain gene was found in either alpha + allele (only a 57 aa Homeobox_KN fragment at 12-18 bits about 1.2 kb upstream + of MATalpha1). LAYOUT: DIC1 (minus) - 0.5 kb (MATa) or 5.0 kb (MATalpha) - MAT + genes - six conserved non-MAT ORFs (409, 173, 589, 219, 465, 622 aa; identical + in both alleles, not recorded) - SLA2 (plus); SLA2 is therefore about 9.5 kb + from the MAT genes. The alpha allele also carries an 800 aa MutS_III-domain + ORF between DIC1 and MATalpha1 that has no counterpart in the MATa allele (not + recorded). No second MAT cassette: miniprot of the MAT proteins finds no other + MAT gene in any of the four assemblies, so the species looks heterothallic with + one idiomorph per genome. GENETIC CODE: standard (table 1). Details: results/2026-10-30_petasospora/README.md.' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: CM115473.1 + seq_region: CM115473.1 + start: 631646 + end: 651160 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] +genes: +- gene_index: 0 + name: dic1 + protein_accession: null + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 631646 + end: 632572 + strand: '-' + order_in_locus: 0 + exons: + - start: 631646 + end: 632572 +- gene_index: 1 + name: MATalpha1 + protein_accession: null + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 637540 + end: 638457 + strand: + + order_in_locus: 1 + exons: + - start: 637540 + end: 638457 +- gene_index: 2 + name: sla2 + protein_accession: null + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 648035 + end: 651160 + strand: + + order_in_locus: 2 + exons: + - start: 648035 + end: 651160 +evidence: + locus_existence: + tier: 3 + experimental_method: 'manual annotation: homology (curated Saccharomycotina MAT + proteins, Pfam, MATa2 profile) and AUGUSTUS ab initio models on the deposited + assembly; no RNA-seq' + citations: &id001 + - pmid: '40558965' + doi: 10.3390/jof11060453 + - pmid: '28385833' + doi: 10.1128/genomeA.00064-17 + boundaries: + tier: 3 + experimental_method: AUGUSTUS ab initio gene models checked against homology; + DIC1 and SLA2 are the flanking anchors, MAT genes lie between them + citations: *id001 + idiomorph_assignment: + tier: 3 + experimental_method: 'gene content: MATa1 (homeodomain) with MATa2 (HMG-box) versus + MATalpha1 (alpha box), at the same DIC1-SLA2 position in different strains' + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: '' + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: manual|36022|MATpf|MATalpha + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/db/candidates/Ascomycota/36038_pb4207_MATbz_MATa-MATalpha/metadata.yaml b/db/candidates/Ascomycota/36038_pb4207_MATbz_MATa-MATalpha/metadata.yaml new file mode 100644 index 00000000..aead7ce1 --- /dev/null +++ b/db/candidates/Ascomycota/36038_pb4207_MATbz_MATa-MATalpha/metadata.yaml @@ -0,0 +1,177 @@ +record_id: 36038_pb4207_MATbz_MATa-MATalpha +record_version: 1 +taxonomy: + taxid: 36038 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Phaffomycetales;f__Phaffomycetaceae;g__Barnettozyma;s__Barnettozyma_californica + lineage_resolved_date: '2026-10-30' +organism: + species: Barnettozyma californica + strain: + name: PB4207 + known: true + culture_collection_ids: + - PB4207 + differs_from_sequenced: false +mating_type: + locus_name: MATbz + idiomorphs: + - MATa + - MATalpha + system: homothallic +locus: + assembly_accession: GCA_023627795.1 + assembly_accession_basis: strain_match_verified + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + assembly_location: + contig: JAMAIE010000005.1 + start: 605238 + end: 630871 + identity: 100.0 + core: + completeness: partial + reference_orientation: DIC1->MATa2->MATa1->MATalpha1->SLA2 (MATalpha2 not modelled) + definition_note: 'DRAFT, GENE MODELS ARE OURS (tier 3). B. californica strain + PB4207 (Dalian Medical University, GCA_023627795.1, ctg_5 = JAMAIE010000005.1, + 694,392 bp; the locus is 63 kb from one contig end). The one published description + is Hession, Byrne, Wolfe & Butler, PLoS Genet 2025 (PMID 41359681, doi 10.1371/journal.pgen.1011814): + a centromere formed at the MAT locus of B. californica, whose 460 bp centromere + sequence lies in a 2.8 kb intergenic region between MATa1 and MATa2 on one side + and MATalpha1 and MATalpha2 on the other; all three Barnettozyma species appear + homothallic. The paper gives no gene models, coordinates or accessions for the + locus (its UCD09 ONT assembly is not in the NCBI assembly database as of 2026-10-30; + the supplement S1 Table was not available). NO RNA-seq exists for the genus + (ENA, 2026-10-30: only WGS and Hi-C), so every model is a prediction from sequence: + stop-to-stop six-frame ORFs, Pfam (Homeodomain, MATalpha_HMGbox, ANTH/I_LWEQ), + phmmer against the curated Saccharomycotina MAT proteins, an exhaustive one-intron + GT..AG search scored with the curated Saccharomycotina MATa2 profile. WHAT AGREES + WITH THE PAPER: MATa1 ends at 615,568 and MATalpha1 starts at 618,402, a 2,833 + bp intergenic region (paper: 2.8 kb); MATa2 and MATa1 are the divergent pair + on one side. SLA2 and DIC1 flank the locus (DIC1 7.4 kb from MATa2; SLA2 8.6 + kb from MATalpha1). MODELS: SLA2 (1,046 aa, ANTH + I_LWEQ, single exon), DIC1 + (316 aa, single exon), MATa1 (162 aa, homeodomain, single exon), MATalpha1 (233 + aa, alpha-box, zero-intron ORF whose C-terminus is uncertain: one-intron alternatives + score the same), MATa2 (269 aa, one 56 bp GTATGT..CTTAG intron at 614,112-614,167, + 82 bits to the MATa2 profile against a background maximum of 32 over all 124 + six-frame segments of the window; no Pfam HMG_box hit, as for Komagataella). + MATalpha2 IS NOT RECORDED: a homeodomain region (genome 620,370-620,700) follows + MATalpha1 but no complete gene model could be built; it is not recorded as present + or absent. NAMING: Pfam cannot tell the MATa1 homeodomain from the MATalpha2 + homeodomain; the names follow position (the homeodomain gene next to the HMG-like + gene on the MATa side is MATa1). The other three californica assemblies (UCD09 + GCA_003123585.1, UCD89 GCA_003123885.1, NRRL Y-17395 GCA_003706655.3) carry + the same proteins at 99.6-100% identity, which is one strain family, not independent + evidence. B. discipulorum (GCA_049863505.1) has SLA2, MATa1, MATalpha1 and DIC1 + at 45-86% identity but arranged differently and no MATa2 hit. GENETIC CODE: + standard (table 1), not tested. Details: results/2026-10-30_barnettozyma/README.md.' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: JAMAIE010000005.1 + seq_region: JAMAIE010000005.1 + start: 605238 + end: 630871 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] +genes: +- gene_index: 0 + name: dic1 + role: flanking_conserved + present: true + segment_index: 0 + start: 605238 + end: 606188 + strand: '-' + order_in_locus: 0 + exons: + - start: 605238 + end: 606188 +- gene_index: 1 + name: MATa2 + role: core_MAT + present: true + segment_index: 0 + start: 613590 + end: 614455 + strand: '-' + order_in_locus: 1 + exons: + - start: 614168 + end: 614455 + - start: 613590 + end: 614111 +- gene_index: 2 + name: MATa1 + role: core_MAT + present: true + segment_index: 0 + start: 615080 + end: 615568 + strand: + + order_in_locus: 2 + exons: + - start: 615080 + end: 615568 +- gene_index: 3 + name: MATalpha1 + role: core_MAT + present: true + segment_index: 0 + start: 618402 + end: 619103 + strand: + + order_in_locus: 3 + exons: + - start: 618402 + end: 619103 + gene_class: alpha_box +- gene_index: 4 + name: sla2 + role: flanking_conserved + present: true + segment_index: 0 + start: 627731 + end: 630871 + strand: '-' + order_in_locus: 4 + exons: + - start: 627731 + end: 630871 +evidence: + locus_existence: + tier: 3 + experimental_method: published description of the locus (PMID 41359681) plus our + own homology/ORF analysis of the PB4207 assembly; no RNA-seq + citations: &id001 + - pmid: '41359681' + doi: 10.1371/journal.pgen.1011814 + boundaries: + tier: 3 + experimental_method: gene models predicted here from sequence (ORF, Pfam, phmmer, + one-intron search); not an annotation of the deposit + citations: [] + idiomorph_assignment: + tier: 3 + experimental_method: 'gene content and position: HMG-like MATa2 + homeodomain + MATa1 on one side, alpha-box MATalpha1 on the other, separated by 2.8 kb (PMID + 41359681); homeodomain names by position only' + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: models derived from the assembly itself + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: 41359681|36038|MATbz|MATa-MATalpha|PB4207 + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/results/2026-10-30_barnettozyma/.gitignore b/results/2026-10-30_barnettozyma/.gitignore new file mode 100644 index 00000000..5b478e6e --- /dev/null +++ b/results/2026-10-30_barnettozyma/.gitignore @@ -0,0 +1,2 @@ +__pycache__/ +*.log diff --git a/results/2026-10-30_barnettozyma/36038_pb4207_MATbz_MATa-MATalpha.yaml b/results/2026-10-30_barnettozyma/36038_pb4207_MATbz_MATa-MATalpha.yaml new file mode 100644 index 00000000..4374c5e7 --- /dev/null +++ b/results/2026-10-30_barnettozyma/36038_pb4207_MATbz_MATa-MATalpha.yaml @@ -0,0 +1,170 @@ +record_id: 36038_pb4207_MATbz_MATa-MATalpha +record_version: 1 +taxonomy: + taxid: 36038 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Phaffomycetales;f__Phaffomycetaceae;g__Barnettozyma;s__Barnettozyma_californica + lineage_resolved_date: '2026-10-30' +organism: + species: Barnettozyma californica + strain: + name: PB4207 + known: true + culture_collection_ids: + - PB4207 + differs_from_sequenced: false +mating_type: + locus_name: MATbz + idiomorphs: + - MATa + - MATalpha + system: homothallic +locus: + assembly_accession: GCA_023627795.1 + assembly_accession_basis: strain_match_verified + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + assembly_location: + contig: JAMAIE010000005.1 + start: 605238 + end: 630871 + identity: 100.0 + core: + completeness: partial + reference_orientation: DIC1->MATa2->MATa1->MATalpha1->SLA2 (MATalpha2 not modelled) + definition_note: 'DRAFT, GENE MODELS ARE OURS (tier 3). B. californica strain PB4207 (Dalian Medical + University, GCA_023627795.1, ctg_5 = JAMAIE010000005.1, 694,392 bp; the locus is 63 kb from one + contig end). The one published description is Hession, Byrne, Wolfe & Butler, PLoS Genet 2025 (PMID + 41359681, doi 10.1371/journal.pgen.1011814): a centromere formed at the MAT locus of B. californica, + whose 460 bp centromere sequence lies in a 2.8 kb intergenic region between MATa1 and MATa2 on one + side and MATalpha1 and MATalpha2 on the other; all three Barnettozyma species appear homothallic. + The paper gives no gene models, coordinates or accessions for the locus (its UCD09 ONT assembly + is not in the NCBI assembly database as of 2026-10-30; the supplement S1 Table was not available). + NO RNA-seq exists for the genus (ENA, 2026-10-30: only WGS and Hi-C), so every model is a prediction + from sequence: stop-to-stop six-frame ORFs, Pfam (Homeodomain, MATalpha_HMGbox, ANTH/I_LWEQ), phmmer + against the curated Saccharomycotina MAT proteins, an exhaustive one-intron GT..AG search scored + with the curated Saccharomycotina MATa2 profile. WHAT AGREES WITH THE PAPER: MATa1 ends at 615,568 + and MATalpha1 starts at 618,402, a 2,833 bp intergenic region (paper: 2.8 kb); MATa2 and MATa1 are + the divergent pair on one side. SLA2 and DIC1 flank the locus (DIC1 7.4 kb from MATa2; SLA2 8.6 + kb from MATalpha1). MODELS: SLA2 (1,046 aa, ANTH + I_LWEQ, single exon), DIC1 (316 aa, single exon), + MATa1 (162 aa, homeodomain, single exon), MATalpha1 (233 aa, alpha-box, zero-intron ORF whose C-terminus + is uncertain: one-intron alternatives score the same), MATa2 (269 aa, one 56 bp GTATGT..CTTAG intron + at 614,112-614,167, 82 bits to the MATa2 profile against a background maximum of 32 over all 124 + six-frame segments of the window; no Pfam HMG_box hit, as for Komagataella). MATalpha2 IS NOT RECORDED: + a homeodomain region (genome 620,370-620,700) follows MATalpha1 but no complete gene model could + be built; it is not recorded as present or absent. NAMING: Pfam cannot tell the MATa1 homeodomain + from the MATalpha2 homeodomain; the names follow position (the homeodomain gene next to the HMG-like + gene on the MATa side is MATa1). The other three californica assemblies (UCD09 GCA_003123585.1, + UCD89 GCA_003123885.1, NRRL Y-17395 GCA_003706655.3) carry the same proteins at 99.6-100% identity, + which is one strain family, not independent evidence. B. discipulorum (GCA_049863505.1) has SLA2, + MATa1, MATalpha1 and DIC1 at 45-86% identity but arranged differently and no MATa2 hit. GENETIC + CODE: standard (table 1), not tested. Details: results/2026-10-30_barnettozyma/README.md.' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: JAMAIE010000005.1 + seq_region: JAMAIE010000005.1 + start: 605238 + end: 630871 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] +genes: +- gene_index: 0 + name: dic1 + role: flanking_conserved + present: true + segment_index: 0 + start: 605238 + end: 606188 + strand: '-' + order_in_locus: 0 + exons: + - start: 605238 + end: 606188 +- gene_index: 1 + name: MATa2 + role: core_MAT + present: true + segment_index: 0 + start: 613590 + end: 614455 + strand: '-' + order_in_locus: 1 + exons: + - start: 614168 + end: 614455 + - start: 613590 + end: 614111 +- gene_index: 2 + name: MATa1 + role: core_MAT + present: true + segment_index: 0 + start: 615080 + end: 615568 + strand: + + order_in_locus: 2 + exons: + - start: 615080 + end: 615568 +- gene_index: 3 + name: MATalpha1 + role: core_MAT + present: true + segment_index: 0 + start: 618402 + end: 619103 + strand: + + order_in_locus: 3 + exons: + - start: 618402 + end: 619103 + gene_class: alpha_box +- gene_index: 4 + name: sla2 + role: flanking_conserved + present: true + segment_index: 0 + start: 627731 + end: 630871 + strand: '-' + order_in_locus: 4 + exons: + - start: 627731 + end: 630871 +evidence: + locus_existence: + tier: 3 + experimental_method: published description of the locus (PMID 41359681) plus our own homology/ORF + analysis of the PB4207 assembly; no RNA-seq + citations: &id001 + - pmid: '41359681' + doi: 10.1371/journal.pgen.1011814 + boundaries: + tier: 3 + experimental_method: gene models predicted here from sequence (ORF, Pfam, phmmer, one-intron search); + not an annotation of the deposit + citations: [] + idiomorph_assignment: + tier: 3 + experimental_method: 'gene content and position: HMG-like MATa2 + homeodomain MATa1 on one side, alpha-box + MATalpha1 on the other, separated by 2.8 kb (PMID 41359681); homeodomain names by position only' + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: models derived from the assembly itself + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: 41359681|36038|MATbz|MATa-MATalpha|PB4207 + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/results/2026-10-30_barnettozyma/MATbz_family_proposal.yml b/results/2026-10-30_barnettozyma/MATbz_family_proposal.yml new file mode 100644 index 00000000..4d0acaa4 --- /dev/null +++ b/results/2026-10-30_barnettozyma/MATbz_family_proposal.yml @@ -0,0 +1,18 @@ +# PROPOSED order.yml family block for Barnettozyma (NOT applied; db/ is untouched). Modelled on MATpk / MATog. +# Scope-only (never searched on a phylum fallback), genus Barnettozyma 599802 (verified with NCBI Taxonomy efetch, 2026-10-30: +# Saccharomycotina; Phaffomycetales 3243778; Phaffomycetaceae 115784). Disjoint from MATsc (4893), MTL (2916678), MATkp, MATog, MATpk. + - locus_name: "MATbz" + vocabulary_type: "enum" + idiomorph_values: ["MATa", "MATalpha"] + idiomorph_class: {MATalpha: unassigned, MATa: unassigned} + taxonomic_scope: [599802] + fallback_subphylum: 147537 + fallback_searchable: false + flank_carried_window_bp: 20000 + genes: + - {name: MATa1, role: core_MAT, present_in_idiomorphs: ["MATa"]} + - {name: MATa2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATa"]} # divergent HMG-like; no Pfam HMG_box hit + - {name: MATalpha1, role: core_MAT, gene_class: alpha_box, present_in_idiomorphs: ["MATalpha"]} + - {name: MATalpha2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATalpha"]} # not modelled in PB4207 + - {name: sla2, role: flanking_conserved, gene_class: sla2_homolog, optional: true} + - {name: dic1, role: flanking_conserved, optional: true} diff --git a/results/2026-10-30_barnettozyma/README.md b/results/2026-10-30_barnettozyma/README.md new file mode 100644 index 00000000..d7a88bb6 --- /dev/null +++ b/results/2026-10-30_barnettozyma/README.md @@ -0,0 +1,38 @@ +# Barnettozyma californica: our own annotation of the MAT locus (2026-10-30) + +Draft only. Nothing is written to `db/`. Everything here was produced from sequence; **there is no RNA-seq for the genus** (ENA, 2026-10-30: *Barnettozyma* runs are WGS and Hi-C only), so the models are predictions, **tier 3**. + +## Literature +According to PubMed, Hession, Byrne, Wolfe & Butler, PLoS Genet 2025 ([10.1371/journal.pgen.1011814](https://doi.org/10.1371/journal.pgen.1011814), PMID 41359681) report that a centromere formed at the MAT locus of *B. californica*; its 460 bp centromere sequence lies in a **2.8 kb intergenic region between MATa1 and MATa2 on one side and MATalpha1 and MATalpha2 on the other**; all three *Barnettozyma* species appear homothallic (both MATa and MATalpha genes present). The paper gives no gene models or coordinates for the locus; its UCD09 ONT assembly is not in the NCBI assembly database; the S1 Table was not available (the supplement bundle holds figures only). + +## Assemblies +Replay MTL calls of v0.7.0 (`results/2026-10-29_near_tie/flank_synteny.tsv`): UCD09 GCA_003123585.1, UCD89 GCA_003123885.1, NRRL Y-17395 GCA_003706655.3, PB4207 GCA_023627795.1 (*B. californica*, taxid 36038) and *B. discipulorum* GCA_049863505.1 (comparator). All are scaffold-level drafts except *B. discipulorum* (complete). **Primary: PB4207** (scaffold N50 421,778; the locus lies on a 694 kb contig, 63 kb from one end); the other three californica assemblies carry the same proteins at 99.6-100% identity (`data/*.lift.gff`), i.e. they are the same strain family and not independent evidence. + +## Method (all scripts here; window = call +/- 25 kb, `fetch_windows.sh`) +1. `run_miniprot.sh`: curated Saccharomycotina MAT/flank proteins (`../2026-10-29_ss_records/refs.faa`) against each window. `run_miniprot_a2.sh`: the seven curated MATa2 proteins (+ *P. kudriavzevii*) at relaxed settings: **no hit** in any window, so MATa2 is not detectable by protein homology. +2. `orf_scan.py` / `run_orf_scans.sh`: six-frame stop-to-stop segments, Pfam (Homeodomain, MATalpha_HMGbox, ANTH, I_LWEQ) and phmmer against the references. +3. `scan_one_intron.py`, `scan_two_introns.py`: exhaustive GT..AG intron searches (one, or up to two) in the region of each gene, scored with the curated Saccharomycotina MATa2 profile (`db/Ascomycota/classifiers/MATa2_saccharomycotina`, supporting evidence only), a Pfam profile or phmmer against curated proteins. +4. `models.py`: the chosen models, checked for start codon, single stop, canonical introns -> `models.gff3`, `models_proteins.faa`, `models.tsv`. `a2_benchmark.py`: strength of the MATa2 call. `make_records.py`: the draft record. `MATbz_family_proposal.yml`: the proposed family block. + +## Models (PB4207, `JAMAIE010000005.1`; 1-based, strand on the contig) +| gene | strand | CDS | exons | aa | evidence | +|---|---|---|---|---|---| +| DIC1 | - | 605,238-606,188 | 1 | 316 | mitochondrial carrier; phmmer 362 bits to curated DIC1 | +| **MATa2** | - | 613,590-614,455 | 2 (intron 614,112-614,167, 56 bp GTATGT..CTTAG) | 269 | HMG-like; 82 bits to the Saccharomycotina MATa2 profile vs a maximum of 32 over all 124 six-frame segments >= 80 aa of the window (curated MATa2 proteins score 144-256; *P. kudriavzevii* 52); no Pfam HMG_box hit; the 56 bp intron beat the next donors (64 and 50 bits) | +| MATa1 | + | 615,080-615,568 | 1 | 162 | Pfam Homeodomain 53 bits, helix 3 `WFTNKR`; 56% identity to *Ogataea* MATa1 over the homeodomain; divergent partner of MATa2 (5' ends 625 bp apart) | +| MATalpha1 | + | 618,402-619,103 | 1 | 233 | MATalpha_HMGbox 33 bits, 62-64 bits to curated MATalpha1; zero-intron ORF; **C-terminus uncertain** (one-intron alternatives with introns of 45-202 bp score within 1-2 bits) | +| SLA2 | - | 627,731-630,871 | 1 | 1,046 | ANTH + I_LWEQ, 1217 bits to curated SLA2 | +| MATalpha2 | | **not modelled** | | | a homeodomain region follows MATalpha1 (genome 620,370-620,700; `WISNKR` helix-3-like, introns of 45-61 bp GTATGT..TAG in the best two-intron scans), but no complete gene could be built (best partial: 104 aa, 22-26 Pfam bits) | + +Agreement with the paper: MATa1 ends at 615,568 and MATalpha1 begins at 618,402, an **intergenic region of 2,833 bp (paper: 2.8 kb)**, with the a-side genes (MATa2/MATa1, divergent) on one side and the alpha-side on the other. DIC1 lies 7.4 kb and SLA2 8.6 kb outside the cassette block (the replay saw DIC1 about 8.2 kb from the call). + +## Limits +- **No RNA, no annotation to check against**: every intron, start and stop is predicted. MATalpha1's end and the whole of MATalpha2 are unresolved. +- **Gene names are by position**: Pfam cannot tell a MATa1 from a MATalpha2 homeodomain. The homeodomain next to the HMG-like gene is named MATa1; the one after MATalpha1 would be MATalpha2. +- **MATa2 identity rests on a profile built from seven proteins** (thin; the profile is not used by any call) and on position; the same was true for *Komagataella*. +- *B. discipulorum* (`GCA_049863505.1`) has SLA2, MATa1, MATalpha1 and DIC1 at 45-86% identity but arranged differently (MATa1 and MATalpha1 adjacent, both minus strand) and no MATa2 hit: the arrangement is not conserved in the genus. +- The centromere-associated 460 bp sequence (S3 Fig) was not checked. +- Genetic code assumed standard. + +## Blocks to a record +The draft `36038_pb4207_MATbz_MATa-MATalpha.yaml` passes the JSON-schema check (`validate_metadata`) and exon bounds, but has no family (`MATbz` is only proposed), is tier 3 throughout, omits MATalpha2, and its assembly anchor is the PB4207 assembly itself (the paper's UCD09 assembly is not public). `curate-db validate` (taxonkit) was not run. A tier-2 record would need the authors' annotation (S1 Table accessions, the YGAP annotation used in the paper) or RNA-seq. diff --git a/results/2026-10-30_barnettozyma/a2_benchmark.py b/results/2026-10-30_barnettozyma/a2_benchmark.py new file mode 100644 index 00000000..f1cd40a7 --- /dev/null +++ b/results/2026-10-30_barnettozyma/a2_benchmark.py @@ -0,0 +1,27 @@ +"""How strong is the MATa2 call? Score the Saccharomycotina MATa2 profile against (i) its own training proteins and the P. kudriavzevii MATa2, +(ii) every stop-to-stop 6-frame segment >= 80 aa of the whole PB4207 window (66 kb, ~ 20 genes), (iii) the B. californica MATa2 model.""" +import pyhmmer +from Bio import SeqIO +from Bio.Seq import Seq +from pyhmmer.easel import Alphabet, TextSequence, SequenceFile +def nm(x): return x.decode() if isinstance(x, bytes) else x +alpha = Alphabet.amino() +with pyhmmer.plan7.HMMFile("/Users/jstajich/projects/MATPredict/db/Ascomycota/classifiers/MATa2_saccharomycotina/MATa2_saccharomycotina.hmm") as hf: hmm = hf.read() +def score(seqs): + return {nm(h.name): h.score for hits in pyhmmer.hmmsearch(hmm, seqs, E=1e3) for h in hits} +with SequenceFile("a2_refs.faa", digital=True, alphabet=alpha) as sf: refs = sf.read_block() +print("curated MATa2 proteins (training + Pk):", {k: round(v) for k, v in score(refs).items()}) +S = str(next(SeqIO.parse("data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.fa", "fasta")).seq).upper() +segs = [] +for strand in "+-": + s = S if strand == "+" else str(Seq(S).reverse_complement()) + for fr in range(3): + aa = str(Seq(s[fr:len(s) - ((len(s) - fr) % 3)]).translate()) + for part in aa.split("*"): + if len(part) >= 80: segs.append(part) +sc = score([TextSequence(name=str(i).encode(), sequence=p).digitize(alpha) for i, p in enumerate(segs)]) +vals = sorted(sc.values(), reverse=True) +print(f"{len(segs)} segments >= 80 aa in the 66.6 kb window; best profile scores:", [round(v) for v in vals[:6]], "median", round(vals[len(vals)//2]) if vals else None) +seqs = [x for x in SeqIO.parse("models_proteins.faa", "fasta")] +m = [TextSequence(name=x.id.encode(), sequence=str(x.seq)).digitize(alpha) for x in seqs] +print("models:", {k: round(v) for k, v in score(m).items()}) diff --git a/results/2026-10-30_barnettozyma/a2_refs.faa b/results/2026-10-30_barnettozyma/a2_refs.faa new file mode 100644 index 00000000..7896d290 --- /dev/null +++ b/results/2026-10-30_barnettozyma/a2_refs.faa @@ -0,0 +1,16 @@ +>Kom_MATa2 +MTVTTFLSSSFDSVAFAMTCVSDKSYKTTAGKGLSGKKFNLSTHRPRNKFIISKSIFSKVIQRYYPDLSFEENSKIASYLWKHRFGCFDKYYEYLQIEESKFTSLVTSKIDQLAFKAVPTEKTTSQCTFRFSSSPKRRRTVNLNILKPLCKRVYSQQFHSPLTGITTQDIFVAEENNV +>Klu_MATA2 +MANSTLRRTTFFKLTTTEDEDTIPKLLQPNNNSVAFPNLKRTGKAFTNDTIKENTKKYTRPRNQFVLMRTLFNRRVNNHILQYYNKSKLEKKMFTLTSKITSELWNESSPDLKSYFSLLATLEENWHKYTHYCSWDRNSAQTLSMEPIELSQVRPRLISSLTVGAGSSVSTYTLRELLLVRKIKSRKRKTTTLTQDSSPTTKFKYKFKKQPKTKMNSNLSKLRFKSKQPPTPPEENSNVFKKRYTSENRIIEDLFLM +>Can_MTLA2 +MPYTFNFPKSQSSFKYSIKSLTNSRSFPMISIYVTPQSTPKFINNQSIDKMTVLSSFRSRNSFIIARSILSKLLRKNKADFKKVSKSVSLLWGSVDNSFKNYFEYLSILESQWHDKKSSNLNPISSTRTTSELNYIELCSSIYNQSRKHPRSVQGNKAKFRLYKNVSKKSNRKPRRLKNGFKVSRSTINCSYGIYTEDVFQ +>Deb_MTLA2 +MSLSPDKIFTFCFKLPRRIDIPVESNQPTTKKNFKIKELTTTNSYPSISINIKQYVSFTSEKYIEASRKPTAKDKKTAITRGSFRTRNKFIIARNLLCRLVKHEKALSSTEISKVVSLVWKESNKSLHDYFEYLSLLETYWYDKLMSSYGNSTLQATATAAVSIRLSSVPYENLCASCYNKHKQKKTLNYDYSKSAKFRVHKDFKEKKKTFLTIKDHSKKLEKLSDAFRINQCTITTNFGIVTEDMFFNSL +>Cla_MTLA2 +MTAEEPLYSRTFSLPSKRKFNKQPTNAKRFPLRATNLISKAPQVDEEFLKRYRSRNQFIMARQLISPFLKGVHNSASRSKLVSCIWKTKTSQFEKYFRYLAALDSNYFEELIKFRSANNAFVGDSYRSMCSSSYSLKRTYRDAKFKVHKFKTEIKRSFIVKQNEYFFGLQVEDIMG +>Yar_mata2 +MENTILHIHSFQLPQTEQPYPEAMLFDRDTSDSRTVLTQKPNGLEISLNFLQYDGHKGLFIRQDSRTNEPEYIEPKVLKPRNSYILFRNATSRCSQSIDPNSAFVSRVSSYIWGSGIPNPIVRRWFKYMGFFEALYHEVDYPEYIPNKLPKSPKKPKVQKGATKSKKKGAKGKNKVTALQVLVGPTVGVGGRMTVSPMTAFFSNNSHVTCYDPNFVLDTPTREFLMMPLDDITLPSQGPRSDSQEQHVPRQPPDGQDYFDILDIDDFVSPYDDLTTRSFTNRQLFKHATLG +>Geo_MATA +MNSFLETPALILYSRKSQLPKKSEKSENVMKRIFSPDSFPSSTSKFNDPREKKKIMSDFLFYDGANNTFSETQAPPRKRKNSTEDKPIPRPTNSFICARSMLHKMFERLNISTIECSNLMSDIWKNYNDDLFKHYFRYLSFLEACWHTSVYPNYRYNPRKKLPSPKPSSCALKKYSKSIIKKRPVKTRAKSSGSTNMMPIKINSCTPGFSFNTEAPRRNYELEIIISELMKEEQLYFGMPIIETAPTSVNCKPFQIDDFSDNFLAFSYLYDEGMRDMSGAR +>Pk_MATa2_202aa +MAKLTDSGSTRNRSESVSQNVGAVEFVQVVKQEHGYDEKEYKKILRSEIEKESKKPRNIFLIYRSLVKQYLMNYLSIKGFTEISEESGKLWKSRSSEVEHYVKYLAEQEERFFENEQTQRLKDRSSSASEKLKEKTLSQLTVDGYNGREPKGETVKKDVFRVYKPKKRKRSGVRSKMKSLRSKQNRVEDIFCVPNIEFQDIK diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.a2.gff b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.a2.gff new file mode 100644 index 00000000..879555f8 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.a2.gff @@ -0,0 +1 @@ +##gff-version 3 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.fa b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.fa new file mode 100644 index 00000000..9fcff93d --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.fa @@ -0,0 +1,736 @@ +>QBLI01000035.1:1-51373 Barnettozyma californica strain UCD09 NODE_35_length_144906_cov_76.032542, whole genome shotgun sequence +GCTGACAGATGGGTTCCAATTAGAGATTTCGATAGGTACATTTAAAGCGAGGAAGCTTGGAAAACAAGAA +CAGCAACGAGGTGTTGGAAAAATTATCGGTGATTACAAGTTTTGCTTAGATTTCGAAAGTCTCGATTTCC +AGATCGCTCAACTATACATGCCCAAGATATCTACTTAATCATTGCAGTCGCGAGTTGAGCATTTTACTCA +ACTCGTTGCAACTCTACCTGAATTATCTCATTTTGGGAGGACACTACCATAATGTACTACCATAATGTAC +TACCATTATTGTTACCGGACTCTCCTCTATTGCGAGTATTACTGACTACCATTTTATTGCCACTCTCTAA +AAATCCTTAGTACCTGTACTACTGATTACCCTTCTATTACCACTAATTATTAGCACTTTCCAAAAAATCC +CTATTCACCACTACTGATTCCCATTCTATTACCGGTAATAGGTAATAAATCGTGGTAATAATTTCGCGAA +TTTTATTATTACCCTCGGTGCTCTAAGATTTGGTGGAGAAGGCCCAAAAAAGATGTACGGCTGTTTAGTT +CCCCCTAAACGGATAAGATCGGGAAAAACAAACGGGAAAAAACGGGAGGAGAAAGAAAAAAGGGACGATA +ACGGATGTGTACAGCCGTACCTTACTGATAAAGCCAATGATAATTCAACTTTCAAACAGATATACACTCT +ATAAATAATCATAAACCAATTCTCATACGCTTCAGCTCCTCTACAACGAGGAAAATGATGATGGTATTTG +GTCCAAGTCTCACAAAACTTGGCACCCAACCTCTAAACATGAAGCTGGGACCTTCATCCTTCAAAGCTTT +AGTCAAAATGTGCACCACGGAATGGTTTGTTCCGGATGCATTCATGATTCTTGTTTTGAGCACATCAGCA +GGTGAGCAAATTGTAGTTGCGGCCAGTCCAGCAAGCAGAGAAGAACTGAAAAATGTGAAGAAGGACTCCT +TTTTCATTCCGAAGGAGATGAGCATTTCCTTGGCCAAATCGTAAGTGACAACTTGGGATGAAGTCATGAG +AACACCTCTCACCAAGTTTGTAGACAAGCCTCTAAATAGGGCACTAGGACCTTCTTCAGTCACAATTCGG +GCCACACCATCAAACGCATTCTTGTAGTTTCTTCTTTGTTCGATCGGTAAACCAGTGTCATTCTGCATTC +TGATATTCACAATGTCAGATGGATTACCAACGATCCCACCAACAGCTCCACTAACCATAGAGATGGGCAA +TAGATAAGCCATTGAACTCATCTTATCGGCTGGAACGAAACTCTCTTTCAAGTACTCATAGGCGCCGAAT +CTGGTCAGTGAGTAGGTAGCTTGTCTCAATAAGCCTGCAGAAAGACCAGAGTAGATACCTGTAATCCCAT +CTGCTTTCAGGATCGAGAACGCCATCTGAAATAGCGATGGCTTGGGTAAAGGAGCGGTCTGTAGACGCAC +CTTAGCCAAATCTAGAGGATGGGTGCAGAAGCAGGCTACAGCACCTCCGAAACCACCATACCAAAAGGGC +CACTTGGTGGTTGGGGTCTTGGTGGCCGTTATGGTTTTAGCGTTGGCGGATGCTGCAGGTTGCAGTGCGA +TTGGAGCCCCAGTGGCGGTGCTATGGGTAGTAGCAGTGACTGACGACATGGCTGACCCGTTGCGATCGAG +TTGACTTCCTGGAACTGGTGTGGTTGGGTTTCAATTGAGATAGGAATTTTTTTTTTTTTTCTTTGGTATT +TTTTCTTTTTATTTTTGAGATCCAATAGTAGTAAACCCAAGCGACAAGCGAAATAGTATATGTAAAAACA +ATAAAGAGAACTCTTAAAGCACTGCAGACTATGTGTCTTCTATGTTAAAACTATAACAATAAAACTATAA +ATGCGATAGACAGGTTGGTTTCAGAATAATCAGGTTTTAGGATTCTACTGGTCTTCGACTTTGCACTAAT +AGCTTCAAATCTTCGAAAACTGTTCGTTTATCCAAACTCTACCTCATCGATATCTCTTAGTCATTTTTCT +ACTAGAGTTCTTTAATGATGATCTTTAATAAAGGGTCATATGGAACAGTCCCTGTAGATTCTCTTCATGA +ATTTCGTGGGGATATGCTAATAATTGCTTATATGGCAATTGCAAAGTCTATACTTTTAAATTCTATTAAA +TCAGAGTGTCGCATATGAACTATGATTGATTTTTAAAGCCGACGGTATATCCGTTTACATTTGATGCGTG +CGTGTGTACATTGGGTAGTTACCCTTATAAGCTCAAAATTTCCAGGTAGCTTGGGCTAAAAAGCACTTCT +TCTATAGTTATAACATTTATACTGCTAGCACTAACTTCTTCTAATATTATTAGGGATATTAGACATGTTT +GTAATACTATTGTTGGAGCTTTGTAATGGCTGACAAAGGCATTATAGTAGGCTTAATTTCAACATAAAAC +GTGCATTTATTTAGCCTGCATGGGAATTAGGAAGTAAAAAAGATATGGATCTCTAGGTGAGTTGATAGTT +AGGTGTATGAGTGAGCTTTATGCCTGGGTCTGAAATTGCCAAATTGAAACCGTAATTTGAGTATCTGAGG +CATCATGGTTCGCACCGTTTTGTAGTGGGGATAAGAAAGATGGTCCGGGTAAGTTCGATAGTTAGAAAAG +AGGAGGGTGACGAGGAGACGACCTGATATCACAATGTGCGCTTTGGAATTGGAGTAGTCCATGACGAGGG +AGAATTTATCCTTGGTGAAAACTTAAAACGTCTCAACTCCCGATAATCGATATTCTTCGACACGGAGAGT +TCAGGTAAACTACTCTATGTTGTTGAATCAAGGATAGTTGACTTTGCTGAATTTGTGGAGGGAGATCTTG +ATAACTCCAAATCAGTAGGAATCAAAGATTAAGAATCATAGCTATAGTATACAGAGTTTCAGAATTCGAG +TAAGTTCAACTTGAATAGACTTTCAGAAACCAACCAGTGGGTAGGAAATCTCAAAGTAAAAGATAAGCCA +AAAAAAAAAGTACATTTTCAAAGTCCAACTGCGGACAAATCCGGGAGGGGAAAGAAGGAACCCCACTTAA +CGGTTCTCTTGATAAAGACAAATGTAAGACGAGTATTAATGACGAAATCGATATTTCCACTAACCAAAAT +CGTTTTGACCTAGTTACTTTAGACCCAAATTAAAATTTACTTACTCAAGGCCACCTGAATTGCAAACCTA +ACGGGTGGGGACTTTCCAAGCTCGCCAAACTCCCAAACAAGCGTCATTGTGTAGTTTATGGTCGATTTAT +GTCTACTTTCAAGCTTCGTTATCTCACGCTGGACTCCTTCGAACTTTACTATTGCTCCGCGGTGACCCAA +GGCATTAGAGGATTCTATCCCCTGCGTAGTTGTATGTTAAACACTGCTTCGATAATGTTTGGGACATTCC +AAAAAAGGAGCGTTACCAAACGCATCAATAATTGGAATAGAGGAGAAATCACTGTCTGAAGATTTCTCAG +TCACATAGGGAGGTGCGAAATTTGAAGCGCCCATACTGGTAGTATCATGAGACAACTTACCATCTGGGAC +CCTTTCAATCTTGTCAACTAATCCAAAAACATACTTCTTTAGAGTCGAAGGATCGTTGTATGACCTTCTG +ATACGCTCTCTCCATCTATCTGCATCTGCTTTCATTGTTAAAGTGTAGGAGAGGCTCATCTTGCCATGGT +CCAAACCAGGTATCTCGGTGATCATATCGGAGACTCCAAATTCTGTCTTTTCAGTGTTCACATGAGAGAG +GGACTCTAATAGCGTTTGGATAACTTGTTCAATAGGTTCCAGGAACCCCTCCGGGATTTCATCCAACCTA +TTTTGAAGATCCTCCAAACTCATTCTCGTGTTCTCCAGCTTGAAGACAGTTTCACGATAGGCAACATCTT +TAACCATCAGATGCCTATGATGACGAATTCTGACCCATGCCCAGCGATAATGGAATAGAATTGCACTACA +AAGCTTAGTCTTTGAAACCAGGTCCAATGGAATTTCCTTCACCTCAATCGAAAGAGCTGTCAAAAGCTCT +TTTAGAAAACGGACAAGCGCAAGGTTGTCTTGCTCAAACTGACAAGCAAACGAATGCTCACCGCCGATCT +TAATAGTGCCAGACTTGTAGTTCTTTAAATCATAGCGGTCTCCGTATCCTTTGTATTCATGTGGCTTGAT +CTGAATGAAAATAAAGCTTTTTCCTGCTAGACGGAATTGAAGCAGGGCTGCACGTGTAAGTGGCTTGGGA +GCACAGAGAACAGTCACAACAGGAGTGCCAGAAATTGCTCCCTGAAATTTGGATATACCTGACAACTCTC +TGACTTCCTTAGGGGAAATAAGCCTCTTGTAATTCTTGCACTGAACCACAAATTTCACACGATCATGATT +TTCATGGCACAGTGGACTCATGACACGGGCCAATTCTCGGGCAAGGACTGGATTGGCTTGAGGTTTCGCA +AAATCAGACATATTCCAAAAACCTATCAGGTCAGCACCATTGTCCCTGGTCCCACCAACGTTCTTCAATT +TGAGATTTAGGGCATCCATCAAAGTCATTCTCACAGAAAGTTCATAGAAAGTACCTCGAATATGCGCACT +ATTATCCAAATCTTTACCTCTGGCCTTCATATCATCGAGAAAAGCCTGTAGAGTTATACTCGTTGACGTC +AGGCTAGACGTGGACATAGTCTGCTTGATGATTGTGGATTTGAATCGGGCTATCCATTTGCAAGTGCAAT +TCATCATTGATTGAATGCACTTGGCTCTCTGGATTCTTGTATGAGCCAGCATAGCCTTTTAATGCAATGA +ACCACTACAGCAGAGACTTAGTGGGACTTGTTCTCTTTTAATATCAGCATCGCTCCACAAAACATTGTTT +CATTGTCTCATATCGAATTATCAAGTTTTGTGATAATATATCGATATTGTTTCAGTTTGTTTTCATATCC +TTTTTTTGAAATAATAGTTTAAGGAGGTACATTGGACAGGTGCTTTTTTAAAGGTACACGTTTTCTCTTT +GAAGATTGGTGATTCAAAATGCTCCCACTAAATTTCGATTAAAGTAACTAATCAGTTCTGGAGCGGTTGC +TCTTACTGTCTCAGCTGTTGGCTGGGAAGAGAAGTTTTATTTTTGTCCGACAATACAAACAAGTTAACAA +TGAAATAGGATACTGCATTATAATATGAAACAGTGAGAATACAACTCACGTTTGGTGCGTGCCTCTTGAA +AACATCTTTGAGCTAATATATAGCTGCTGTGGCGAGCTACAACTACCAGCCGTCCAACCTGCCAACTGAT +AGGTTCCAAGGTAGCAATTAAGATGTAATAGGTGCATATATTTAGCTGATGATTGTGAATACCACGATCA +CATACATACACCCTTCTGGGAGATTTAAGGTATTTTGTCTGGCTCTGCCCCATAAATATACGTTGGTCGT +AGATTGCTGCTGATTAAAATCTATCACGTGATCTTTATCAGTGGCCTCTGGGCGCGTTTATTCGTGGGAT +AGGCGGGTAACTTTAGAATGAAAGTCGTAGTATTTTTTTTTAAGACGCGTTTCTAACGCGTACAGTCCTA +GTCGCACAAACAAAATAGGGTACAGACAGCCAAAGAGTTCGCTTTGCTAAGAATTTTACGTGAAATGGAT +AGCTATATGACAAGTGAAAACATCCAAAAGCTAATATCTGATACCTGGAACTCCCAAACAGGATCAGATT +GATTTTGGAAGCAGAGGGGTTTAAGAAGATTTGAATTCAAAAGAGATCACTATCCTGAGGTGCAATGCAG +ACTTAGAGGCGGTAGGTTTCCCACACGTGAGACTCTGACGATCACTCCATACCCATTGAAAGGGGAACTG +ATCACTGAGCCTCTCTCATTGAAGAAGCCGAAGTAAGCAGTCTTATAGACAGCGAAGATTGACTTATCAA +TTATGTTATGTCTATTACGGCAAACGTAAAATTGAAGCCCGTATACCAAATGCTGGTTGATATACCGGTA +GATCATCGCAATGTGATGATTCTGGATAATTACTCCGCATGGATTATTTTGGCTGTTCCGTTCGGGACCT +GATAATACCAAAGAGGATACTATGCTGCTTCAATACAAAAGGAAGCCTTTTCGGCATGGATTTGTGGATA +GCACTTTTGACCACTGGGCTTTTAGATTTTGGTTTCCAAAAGTCGAAGAGCATGTCAAACCTGAGGAAGG +AATCGGACGTGTTGATTTGTCAGCGGTTATGTAACTTGACACAAAAATCACACTTTACCACCAACAGCTC +CACGAGTTTTAACGACACCGTCGATCTGCGTGCACCATGGCTCGAATCGCCGTCAACTCTACCTGTATCA +ACATTTACAACAGTATGCGTCTACAATTCCTATTTCTCCACTTGAAACACAGATTTGTATTCGAGTTGTG +AAATTTATTTCAAGAATAAGGAAATCTTGACCGCTGGAGCAGTACCTCATAGCTTAGAGGGCGAAATCAA +GAGATTTAAGATTCAATTTATCAGGGTTAATAGGAGATTAACTTGGAGGCAAAACCCGAAACAGAATCAT +AGTCCAAGGCATGACCGCGACCAAGAAAGAAAACCAGTTAATGAGACCTCCAGTAGCATCCTTGAATCTT +CACATGTTCTAAAAGCACCAGTTCATCTACACAGAGATAGGTTTCAATCGATTTGCCTTAGCTCTCTCCT +AGATCCAAAAATAACGGAAACAAGAAAACTTTCCAACAAGCAACCGATTCCAAAATATTAGACTCTGAAG +GCAATGGTGCTAGCTCAACGCGGTGGAACACCCTCATGTTGAAATGGAGTGTTTCCGGCGCTGTATTACA +CAAGAAGACCTCGAAGCGCAGAACCATATCATGGCTAGGATGTGCCTGAAAGCTGGTAAACCTAACCTTT +CGGGACTAAAGATCCTGCGTTCTGTCACCTACTGTTGGTATTGATTCATACCACAACAAATAGCTGAATG +ACTAATTTGAGCCAAAGCCAACCAACTCCACGGGTCATGTTTGTACTTTTTGTGCTTATATGGTACGTTA +GTTAAATTGCTACGTACGAGTAGGTATTTCTCAGCAGGATTTTGACGCTCGTTACCCTCATTACAGGATA +CAAAGTGTGGAAGTAATTACTAATATTAGTGTTGAATCCTGCATACGCAAAGAAGCCTTTCCTATTCGTC +CTTATTCTTCGTACCCACCAAAGCCATGGATTGTCTGAAGTTTGCCCTGACCTAGTCAAAAGCACCCGTA +CTATACCTGAGGTGATTAATTGCATATAACAGGGATCGATGAACATGCTATCATTTGACGTTTTATCCGG +AGTATGTTCAAAATAAGGACTTGGTTTAATTTAGTAAAGGTTCAGTGCTCGATCATGGGGACTTTTGTTG +GTATACATGCGCCTTGTATTAATCGGCAGACGAGAGGTGAAACAGTGTCAAATCGGAGTGGCAGTAATTT +TATGCCTCAGATACGAGAGAAGCGACATGGATCATGCCGAATGGAGTTTGCTAGTGAATAGCATAATAGT +CCCATGCGTATATCTTCTAAAAAGCCGCGGATCAGAACGAATTGAATTCTTGTCAGCTGCTAATGCCCTG +AAAAATGAGACGAACATCACCGCTGTGGAAGGAGGAACCACCACATGCGTGTTTGAATAAATCGAGATTC +GGCTGCTAGTTTTAAGCTGGCCCCTATAGTTCAAATCCTTCCAAGTTGTGAACTCTTTCCGAATGTTTGG +CATTACCAGGAACGCATTGCTAATAATAGGCAATTTAGAGCAAAGAATCTTCAACAATTAGAGGAGTACA +TGAGGACCAACTGCATAGAGGAATTTGGATAACCCATCAATATTCGTGTAAATATTCCGGAGCTACATGA +TTTGTCCACATCTTTTCAACTAGCACAGCTGCTTATGAGTCGTTATTGCAAACCAGAACACAGCAGGCAG +AAAGTTTTACTTTGCAAGAATAGTGCCAGCAAGAAACATGAATGGCTCTTGCTGCCGCGCTATAATACTA +ACAAAATACTTTTAGAGAATGTCAAGAGGTCAAGATCCTTCTGATTGCCAAGCTAGATAAGAAATCCTAT +AACCCCATAGGAGTGATAATAGAAGAATTTGATCTGCCTACTGAATGTGAGATGAGCAATATTAAAGTTA +AAGGAAGTTAAAAGCCGATGAATTAACAGCGAGTGTGGCATAATGTGGTAATTTTAAAGACATAATAATT +TGTTTGGCAAATTTTATTCTGATCTCAAGACGGAGATAACTGAATATATTCAAGGATAAGCTGAGAGTCA +AGATACAGAGACAGTCACGCAGATGCTTTCCAATTTGAACCAACATGACTTGTTTTGTGACTAACTAACA +TTCGCCCCCGGGGTGCTCAAGTGATATCTTCTCAGGTAATAGGATACCGGTGGACTAAAGATTGAGTGGT +TGTTCTCATATGGAGTACAAGAATCACACAGATCTCGAGGACCACAGATCTCGCGGCTCTTATAAGTTTT +CTTTAAATGCAGTAAATTATTTCGCTTTTATAATCAGGACAGCCAAAGTGGTGGACATAATTCTGATTAC +TGAGATCGATATGCCTGTGCTGCTACAAGTGCAAGCAGTAGCTAGAGAAGTTGCGTGATTGCTGTTCAGA +ATTCGTATTTTGTCCTATGCATTGCTCATAGAACCAGCCTTCAAAGGTTAGTATTCCAGTTATATAAACA +AGTGGAAATTGCCAATTTGGGAAGGGCTAACATAAAAGATGGTCAATAGTTTATCGATTGTCGTCGAAAG +TGTCTTCATTTTTTCAACTGATAAATGTAAAGGCTGCCATAATATTACATGCCATACTGCAACTGAATTT +TGGTGGAAGAAATCAAGATCGGAGAAACCTTACAAGCTACAGTTATCTGAGTTTAGCACACAATGCCGTA +AAAGTTTCTGATCACCTATGAATCCATAATGCAGTAAACAAAAAAACAAGAGAGTACTGACCGCTTGAAA +TATAAATGCAACTTCAAATTCTCCATTGTCTTTTAGTGGGGGCTTACAGATCAAAGTTTAATATTTTGCT +ATTGTAGATTGTTCATATGTAATCCTTAAAATCTTCTAGCGTTATGTTCTGAATCTTATCTTCGTAAAAC +AAAGGTTCAAAATAATTTGTTTTGTCAAAGCCACTGAGACACCAGAGATTATTTTTTATATAGGCATCAT +CCTTTGAAGACCTTGGCCCACGCTTCTTATAAGTTGTTCCTGAAGTGTGTCCCTTGGTCAGTCTAAACTT +ACTAACAAGATTAATACTCACATTTGAAACAACAGTTGAGTGTCTAAAAGAGCTAGTTGATAATTTAACG +TAGGTGCCCATCATGTGAGGACTTGTGTTGACAAACCCAGATTTTTTCTTCAAAGAAGATAACGGGGCAC +ATTTGCTTATCCGCCCAGTCATAGTTCTCGATTGCTCGAGTACTGGTGCTAAGCTTTTAATTGTCTTGGA +TCTGACCTTAGGTTGATAATGGTAAAGTGGATAATGCTCTTCGTGCCAACTTTCTTCAAGAAGGGATAAG +TACTTGAAATATTTTTGAAATGGTGAAGAAGAAGTTTTCCAAATCTAAGCATGTTAGTAACTTTTAGACT +CCGTGCAGGAGAGGTGTTTCAATTACATACTATCGATATTATCTTAGAAACGTCATCATTGGCAATCGTA +TCGTTGCAGTTCGCAAGGACTATCTTATTCAAAATCATTCTTGCCACTATGAACTGGTTTCTAGGACGAG +CAACATTCGAATATTGCTTGCTCGAATTAGAAGGTGAATCTTCGAATGTTAAGGTAAACTTCTGGCCACT +AGAAGGCCCCTTAGGAATAGAAACAAATGGGAGAATACTTTTCTTAATTCGCAAATCAGAGTCATTGCCA +GCACTGGCCTTGAAATGTGCAATCTTAAGACTTTCCATTATTAGTACCGTACTTATCTTCGACGAGTATA +TTAGCAGATATTTTAAAGTAACAAGTTAAACATAAAAAATCTCACTTCTAGGATGTCTGTTAGTTTTTGA +GGCAACAGTTTCGAGTGTGGTTAAACAATGGTAGGAAAAAAATCTAAAACCTTGATTACGTTATCTTTCT +TTGCGAACTAGAACTCCTTAACTAAAATCAAAATTTGTAACTTAGATCACTATATCGTAAACTCCTTGTG +GGTCTTTTATTTGACACCTTAAATTTACAAACCTCTTATCATAGCCCGCTACTCAATCAATGTCGTACTC +AAGTGATGACTTTGATGACTTCTTTTCGCAAAACAGTCTCGCACAGGCTAGGCAACAACTTGCCCTTTTG +ATAAAGGATGTTTCAAAATTGGCTGGAAATATTGTCCCTCCTTCTAGAAACGAGGTTGATGAAGTTAGTG +TCACTGATCCCTTTAATAGATCAAGAACATTACAAGCTGTTCCTAAGGCCCATAGATTTTATTTGGCGAA +GCTCTTGGATGGTTTTGAAGAATCTTTAATTCGAGTTTTAGTATGTAAACTCCCATTGATCCAACTAAGC +TTTACTAACAACTTCTCAAGTCTCTTAAGGGCATGAACCAGAAACTTATCACAGGTCAGTATCAAAGGCT +TTTATTTAACACATTGGCAGACAAGTACGGTTCTGAGGTCCTAGAACTGATTGACTACAGTGTTGAAAAA +CCATATTTTCTTAGCAAGTCTTACAGGGTTAAGTCTTCAACTATTTCTGTGAACGAAAGAAGCGCAACAG +AATCTAACTGTTCAGACATATCTCCATCTTCACATTCTCCTCCGGAAGCATCATCTATGAATACTTCTAA +TGTCTGTTCTGTTTCTGAAGCAGTTATGGCCTCACCAGACAGTAATAACAATATTCAACAAAAGAAGCGA +AGAAGACATATTTCCAAGGAAGCTCGCAGCCTTTTGGAGAAAGCATTTGAAACTACTAAAAATCCAACCA +GAAAGGAAAGAGCAATAATCGCAGAGAAATCCGGTATGACAGCACTTCAAGTAAGAGTGTGGTTCACCAA +CAAGCGTATGAGAACCAAGACCAGAATATAAGTTCATGTTATTGCCTCTGATTTGTAGGGCGCGTTTCAA +ATGTAATTCATAGCCAAACATCATGTCTTTTGTGACTCTCACTAGCCATCAATAGCATCTCCTTACCATT +TACTACTTTGCCACTGTTATCATCGATTCTGTTTACCTTAATAGCTTAGAACATTATTGACTTGGGTTTC +ATCATCTGGAAAATTTTGCCTATCTCTGAATCATATCTTGATAATAGTTTACCTGCTGGTGAAACACTCG +AAGTTATGACGAGACTAGTGGGCGTTTGATGTGACAGGTTAAACTCTCACCTTTCTTGTCTTTAAATCCA +ACCATTATATGTATCCCAATGATATCGAAGTGGAGCTAGGTTAACTATCTTGCAAACACCAACAAACCTT +CATCTTTCGTCTATCGTACTATGTATATAATGTTTATTAAATACAAGATACTTCGATCTCGAAGTATCCA +TTCATAGATACCATTACTCGTTCTTGTTAGAGACTTTTGATACGGCTCTAAGTATACTTAGTTTAGTAAT +CTTCGCTAGTGGCTTACAATGTGACACTCTAACCATCTGTTTTCATTGGGCACTAATCTTCGTAATGAGC +ATCTCGATTGGGAGACTTCTGAACTTCATCTCCAATATTCCTTTAACTTTTGTTTTAAGAAGCTTTGAAC +ACTATTTATTATCTTTATCTGTGTACCATTATTGAATTGAATGGCCTTTGCTGGAAATAGTAAACAGCTA +CGTTGTTCGGTTTCCCTCTCCCCCAAATAGTTTTAAGTTAAGCCTGTAAACTGCCCTTCCCGCCCTTCCT +ATTTGCCAATCCTCTTACGTTTACTACTTGTAACTTTCTCCGAGTACAATCGAGGCGCGCCCAAGCTTCA +AGTTCAAGAACAAGCCAATTTCACATTATTAGGTTTTTCAACTCTTCTCAAATTCCTTGCGGCTTCCTGA +CAGTTTATTTGGGTTGTTTGAACGCAGCCCAGGTGTTCTGATCTCTCTTCAGAGTTAAATTTAAATTAAC +ACCTATTTCGTTCAAACGGCTGTACCAAGGCCGTATTGTATAGCTGATTAACATGTAGTAATTGTGGAAT +TTGGAGCTGATTGCGAATAATACTGTAGAATAAGTTCTGTTTTTACTGCGCCCCCGAGCGGTACAGGAAC +TACTAATGCACCCATTAACATCTACATGACCTAATAGTTGATGCTCCTGTCCTTACTGCTCGCCGGTTGC +CACTCAAACAAACTTCTGTGCCGCCCGGCTTACAGCATACAGTCGTATTTAAGAAAGTTCTACATCTCGT +TCGAATCACAGGACGTTCGACCAGAATTTGATTTTTGGTGTCGTTAAAAGAACAGGACGCCATCCTTTTT +GAGCAGCCATTGGGTCATAGAGCTGCATAGGAGATAGTTTGACATTCTAATCGTTAAGATAAACACTAGC +TCTGATGCTGGGTCCAGGTATTCTCACTTCCGGGAATATATGCCCTCGGAAGTTGACCCCAAAGAGGTGA +TGAGAATTACACGTGGAATTGACAATCAGACTAAAAACTACACACATTAACTAGCACCTAAGGGAGTGCT +CTCATCCTCAATGGCTTGTAAATTCTCAGCTGGGGATGAACCAGGCTTTTGCAAGGTTATAAAACTGTTA +TCGAAGCTGTGATGAATCCTGCGTCGAATCTAATTCTGGATTTGAAAACTTATAGAGGTAGTCAACAATT +CCCATTAAACAGGTGAAACCCGAATAAACCATTGAGCTTAATATGATGTAAACAAACTCACATATTAAGA +ATGAAGCCAAATGTGGAATGTTGTAGCTCCCGTTTCCGGCCTTAATTCTTGCCCTTGATGGTTGGAAATC +AGGTCGAAGGAAGATTGGATGTTATGAAGAGGCAGAAATTCTCCAACGCCCAAGAAGTAACGCTAAGAAG +ATCCACAGTGTTTCATTGAAGCTGCGAGAATAATAAAGGTGGCGAATATGAGATCATCAAGAAGATGTCG +ATTTTAATAAAGTCCTTCTATTCAAAGCTTCTCGTCAAGATTAATATAACGCCAGAGAAAGTGAGTCCTT +CCCAGCGACAACCATAAAGTATAGAATCATTAATTGTACGGTAACAAACTATGAGTGGGGATAGGCAGGA +TACAGGCGTAGTTAGCAGAAGGTGTAGAGTTATGATTTAATAAGGAGAAGACTGCATTGATATACGGTGA +TGTTAAGAAACGAATGTATCAGATCAGGTTATTAAAAAAGCGGCTATTTTACGGTCAGACTTCAAGAGAG +ATTGGATGATTAGCATCAAACGTAACATTCGTAACATGCGATACAATGCAAAGCATAGGCAGGGCAAGCT +ACAGGAGCACCTCGAGATTATTGTGTTTTCTATATTTTAGTCTTCCCAAATTGTTATAGGGGGCAGAGCA +TTAATATACTTAGAGATACTTGTGAGCGTGATGTGATAGTCGTTACGAATACGAACCAACACGTTTGAAA +TGTTCCGAATGGATTGAAGTTAAAGTGCTGCTGCTACTGCAAGTTTACTACTATTAGCATCTGTCAAAAT +GGAACTTGTAAAATAGACGTTGGAAGAATGGACAAACTAAATGGGTAGCATTGAACAATATGTATGGTTA +TTAGTCGAAAACTAGAGATTCTATTGATATTTTCAGGATTATTTCCATCAAGAATTTTTTGTAAAGCACT +GTTTCGCGAACGCAGACCTTTTTGAGGCGTGGCTTAACGATTAACCTTCCGGCTTGACGGACACGATCTA +TTGAAGAGTGGTATGAAATTAACCAACTGTATATACATATATTCAGTAGTTCACGTTGATAAACAATGCC +TAGGAAAGAAGTGAAAGCAAAGTGGACAAGCAACCCGAAGAAGCCATACAGTGCTGGATTTAAACTCTTC +TCTCTTCCTGGACTGAAACATCGCAATAATGCCCTTGAAAGTAAGAACCCGTCAAAGTGTACCTCCCCTT +CGAATTTTCAGTGTCTTACTTTTGCCGCTACGTCCCTACCAAAGATACCAAAGCTTTCAGCCGATTATGA +AAGATTTATTGAAGGACTAAAGGATCATAAAGCTGGTCAAGATAGTAGTACGTACATCCGCCAACATTTG +AGGAGAAAGAGAGGGCCCTCAAGAAAGCGGACAAAGTTGAATGGTTTTATGGCCTATCGATCCTTCTTCT +CTAGGAACGTTGCAACAGATCAACAAGGAGTCCTATCTTCTCAATTGGCGAAAGAATGGGAGAGGAATCC +AGAAATCCAACTTGTATGGAAGAGATATTCCACAGAGTACAATTTGTCTGTTTTGAGTGATCGCCTTCCC +TTTTTCGATTGGCTTTTGCTTGTAACATCAGAGCCTACTTGTATCTCTGAAAACATTTATGACAACAAGG +ATCCAGGTACATTCTCTAGAATTCAAGATGTTTTCAATACAAATGAGGCAGATGAGAACCACCAGTTTCT +AAAGATGAGTTTTGATGTATCTGTGATCGATCCAAGAATAGGACAAGCATTCGAGCATTCTATATAGTTT +ATGCTAAACCGATAATTGAATCGAAGCTTATAACAATTGAATTACTGTTGTATTACTTTTATACATTGAC +TGATCTATTGTAACCAAACAGTTAGTTCGAGAGTTGATACAGGAACGTCAATGTAGGATGATGCAAATGG +GTTAGCATCTAGTAATGTTCTACCAAAGCCATCGTCTCCTCAGCGTGTGAACAGTATTATTAGAACCTTA +AGCTACACCTAACCTTGATGAAACTCAGTTAATGTCACTTCCAAAGTTTGAGTGCTTCTTTATAACAGCA +GGAACGGCTCCTCGCTTTCCTTAAGTCGATAAGCTATTTCCCCACCTTGATTATCTTTTTGAAACAAACA +GTTACGGCGGTTATTGTTATTTTTAATCTGAAACAGAACCTATCAGTTATGTGAAAGAATACGCCATCTA +TTACAGTTGAAGTTGACCTGAACATTATAAGGAATAGGGATAACCTACATTCTTACTATTAAATATTGCA +AAAACGCACCTAAGATCTTAGTTTTTCTAGCGATTATATGTGTATATATATTCGTTTCTTTAGCTGTTGC +ATGATCAGTTGTTTCATCTCTATCACGTGATTTATGTTCATGCTGTCAAGGGATTTTGAGCAGTCTTGAA +ACAAACTTTCTCTAGCTCCCTGTTATTCTATTACTACAAGTTCACTTCCTGCCTCCAAACAATGTCTGAA +GATCAGTTTAGCAGATGTCCAATAACTTCATTACTCTCAGACACTGATAATATACAACAGGGGGATACTG +CAGAGTATGTTCTTTTTGAAACTCTTGCAATGCAGATTTGGTACTAACAATTAAATAAGTTCACGCTATT +TACTATCCTCGAGCTCGAAACATATCCTAGAAAGCTGCTCACTTATTAGCTCCATTGATGTTGGCACACT +TTCTTCGAACATCCTAGCCTCACATATGGTAAGTTTTACTTCTATGTCAAGCGATCAAACTAACACACTA +AGGAGTTGGATTTACTTCAGAAAAGACATTCAAATACTACAGCAGAGGATCAGTCACAGTTTCAAGAAAT +TCAAAAAGACTTATTGAAATCTCAGCTTTTGCTCTCCGAGAAGGCCATCTTAGTCGAAGCCCTTGAGCGG +TTTTCAACTTCGACAGAATCTCCTAACAATCCTGACCCTTTTAGATTCAATATTATTACGCAGAAGATGA +TTAGAGAAGATGGAAGTATTATTTCTAGAAAGAAAAGATTAGCAAGGCAGAAAGTTGCCATTCTTGAGGA +TTGGTATGTTTTCTCGCACAGTACCCTATTCAAATTTACAATTAACATAGTTTAGGTATTATAGTCATCC +GGAGAAGCCCAACTTGAATGAATCAATGCTTCTGTCTTTGGTCAAAAAGACAGGTCTCACTAAAGGCCAA +ATTCGCAATTGGTATGTGTAAGATTGTCAGAGATCATAGCATGAACTAACTTCTATACTTAGGATTTCCA +ATAAGAGGTATGTGTGCACTTCAAAGTTTGTAATGAATCGGTACAGTACTAACAACATTGATAGAAGAAA +GGAACGAGAGGACAAAATAGGGGATTCAATTTCATGTCTAGTGTCGGGACTTGTTAAAAACGATTGTCCC +TAAGCCTATCACTCCAATATGTGAACACTTCTCCTTGCACAAATTACTAGGAAATTCTGAAATTGTTAAC +ATTGCATCAGAACTTTTGAAACCTTCAGGATGGCAGCTTGAAAGATACAAGCTTTGAGAATCACTATTTG +ATCTATTTCATTTGCTTTCTCCCGACTGTAGTTTTCTGTTGTTACAAATTTTGTCCCTTTCATGAAAGAA +TTCAACTCAACCAATCCTTTTTCAGTTATCACTTTCATGATATTCCACTCCATGATAGGACGTGGTGAAT +GTCTTTTAACATATAAGCTTTGAGTCTTCATTTCAGCTTCCTGCAAAAATTTTAATGGGGTGACACCACT +TTCTCTAAACTTGCAACACTGTAATTTTGCCTGAGCGACCTCTACGCAGTCTCCATATACTTCAATGGAG +TGAGTGAACGCCACCCGTGGTTGAAGAAGTGATCTCACATACTGAACGTATTTTCCTCCATTTTTTTTCA +TGTCAGGTACTTCAAATATTACCCTTCTCTAAGACCATTAGTAATCGTAGCCTTCGCCAACTATATCTCT +CCTCGTCATCAACAGTCAAATTCAACATTCTTGAATCCAGCTCCTTTATAGGAACTCTGTAAACCATTTA +TCTGCAAGCAAAATTAATCTTGGTGTCATCTGTCAAATTCCCCTAGGTTCATTGCCTGGATAAACAGTGA +TTTTAATCTGATTCGTTGTGATTCTTCTCATGGAAGAGTTTTCACCTCCAAACTTTCCGAGGTGGGATGA +TTTTTCGATCAAGAAGAGATCAGTTACTGATGCTGAGTGACATTTTATTGATGGATTTATCAGATTCCCT +AGCCACTTGTGTCTGGAATTAATCAACGGAGTTCGTTCACTTTAGAGACTCTTCTGGATAGTCCTCCAAC +CGCCACTGTTGCTACTCTTCTCGAATTATTGGTCTAGTAAGTGTTGTTCAAAGACTCGACATTACTGGCG +AATATAGAAGGCGCCCAGAAGTCCACACTCAATAATAAGCCTTAGTGTCGGACCTAACGGTCTGACCTAA +AGTGCAATAGTCTTTTTTAGCTGCCGTATACCTTTGTGGTAGGATCTATCCCTCCCCAGTCTTCGAAAGA +GTATAGTACATATGTCTTCCTCACCAAGGTCCTGAGCTATGCGTTAAAGGATTATGATATCCTTAGTAGT +GTTTGGCACATGCAACTCATCCAAGGACGGTCCACATCGCATGGTCGTACTGGCAATTGCATTAAAACCA +CTCAAGAAGGGACTCTCCCTGCCTGGCGATTGAGGTTGTGATAGCAACGTTAAAGGACGCCCATCCGTAT +CTATGGAATTTGTGCCCATAACCCAGATTCGTTTTCACTCACTAATTTAGCCCTAAATGGTGGAACGAAG +ATGTGCAACATGAACATTATCCTTTTTCCGTTCAGTCTTATAAAGGCGAACTTGAATGGCTTGCTTCTGA +GCAATCAGTTAATTGAAACAGATCTTAGATGAATCTTATCAGCAGTTTCACATTATTTAAAATTGCTTAG +ACCTAACCTAGATCTACCACCGAGAGTTTTAAGTCTCATTTCATCAGGCACTTTAACCCCGATAGACTCA +CCTCGAAATTAGAGCTGGTTTATCAATGTAGGCTTCTGACTTATCACCCGATCTCAGAATATATCTAACA +GGAAACATCGCCACACATAATTGATACCCTAAGTCCATTGATGCAAAGTTATAATCTATCCGACACCCTT +CAGGTCATTGGTTTGGGCGCTGGTTTTGTGCAATACCACTGCCATCACGGCCAATTGCTGTTTATCGATT +CTTAATCGAACTCTTTATCATGACGCGTCATCTTCCATATCGCAGTCTATGGCGCCACCTTTTCTGCAAC +GAGGCACGCTAGTTCGGCTAATCTAAGTCCCAGAATGCTCCCTCCTTCGTTTTGCCATTGCTTCCAATCA +GAGCATTCTTTCTATGTGTGTCATCAGTATCTCCAATATGTTCTTCCGGTCAGAAGTAGAGGTCAACGAT +AAGTTTTTCTGAAATAGGTCAGAAGGTCAGAGGTTCTATTGCTTACTAATATGCTGGTCAGAAACAGACA +TTATCTTCGGCGGATGTTGTGTATCTGTGATTGGAAGTATGTTTGTATTTAAACTGGCAAGTAGAATTTC +ACTAATAGAAGTGTCTGCTTAAACTAGGCAGTTTACAGAATAAACAATTTTCTTTACCAAGCGCGTTGGT +CGGATTCTGTAAACGCGCGAAAGGAACCGACGCCAAGATCTGCAATATTTAAGTGGAACGTCCATCGAGA +TACCTGAATATGAACTGTTCGTGAGTATCCCGATCCCAGCTTCCTCACCAGAAAACTCCTGGGGCTACTT +GAGATTTGGGCCATAAGAATATTTAATATCTTTATTCTTGAACTGTTCATGCTTCTTCTAGGAACTTTCG +AATAGCTCATCACCATAGTAGAATTCTGGGGATCTTTACTTTTGGTGCAGTCCTCTGGTTTCCCATGCCA +CCTCTCTGTTGGAGGGCGCAGTGACGAAAAGGGTAATCAATATTCAGCTCTTGCAGTATTCTGCAGTACG +TGGGTTGCCTCAAATAAGGTGCACGCGTAGATGTGAGACAATTGCAGTAGGTCAAATGGAGCTGATGCGG +CATATTGCATTTGGGCTGTACTTCTTTCTCTCAAAAAGTATTTGCCAACGTTGTACGGAATCGAATCGGT +CAGATATCCCTTTACAGATTATCAAAAAACAAGATGCATCAACCGCTATATTGTTTTTGACGCGTCTTTT +AATACTTTGCTCAGTGGTAGGCAGAATTACACACCGTGTATACCTATTGTGTCGTGGCCATCATTTTCAT +TGTGAGGAATCTACCTCATGGGAGTTGGCATTTCTGCGATCGTTGTAATTTCATGTGACCCGTTAGTTTC +CATCCTCCACTTTTTTGGGGTCATGAGTATCTTTACCGGTTATACTCAAATAAACACTCAAGTCTTATTC +AGGAAAAAAAAAAGACTGTTTGTTTGCTCCCTGTGACGCACGAAGCAAGACACAGGGATTGGGTGACTAG +AAGAACGTATTTTCTCAACAACTCACACACCATATACACTTTATCATTATCCAAGAAAGCAAAATAGGCC +TTTATCCATCCAGGTACGAAATTAGAATAGCTTTATTTCCTTTTCATGTCTGATTTCTATCTATCTCTAT +AGAATAATAGTCGTGGGTGTCATCTTTCGGGGTAATCTCCACATTTCCCAATGGTCTGTTTATTTGCGAA +ATCGGAACTATCGTAAATATCCCAGTGCCTCATTTCAGATTGTCTTTAATGCTGGGCAAACAGTTCAAAA +TTGCGTTAAATGAAAATATTGACTGTAGACATAAAGGAACAATCCAAGTTACACAGCCATCTCAGCATCG +CATGATTTCAAAATCAAAGCACAAATCAACTCAAAGTTTGAAGCCAGTTGCCTCTTTTGCTGACACGACA +CTCTCAATTGTATAGCCCGATATGTTGTGATTTCAACCCCAATGATCTTCCAGTTTCGTAATGTGATGGT +TTTTTTGAACATTTCTCCATCCATTTGTGGAGCAGGGAAAATATATACCGCAGCTTGTTTAGCTCATACC +TTTTCACCAGAAGGGTTCAAAATGATCCAAATTTTTGTTTCCAAAAGTGAATTATTTAAGCTTAAAGATG +TGCACTCAATTGTTCACTATTTGTGTGTTCTGATTAGCCAAGCACATATCATCCAATCTCAGAGGCTTTG +CGCAGTAGCTCAAACAATTATACACAAAGACGATTTTGATCGACCTACGACTAAGGAGAAATGAAAGAAA +CAAGGTGCGCTAGATGTATCAAGACACAACAAACATTAGGGTACGAATGTTAGAGCGAAGTACAGATCCC +AAATTTAAGCAACAAAGTTGATGTCTCAATATCCTCCTACTCATCCAATTTGATCAGAAAAATATTGAAC +CAATAGCACAGATGTCTGCATTTGCTAGGGCTTTATCATTTCAACGTTGCCCTTTGTGATATCTCTATAT +ACGCCAAAACGATAAGACATTTACATATTTAAAGATTATTTTTGCTCATATCTTTGGTCATTAGTTTGTG +GATATCTTCAAGAGCTCACTTGCTCTAAATGCAATAGATAGCTTTTCAGTTCGTCTAAGAACAAATTGAA +GATTTCGACATTTGGAAAGTGAACAGTCTGTAACTCTTTATCAACTATGCTGTCAATACTGGGACCAAAT +TTGCTGAGCCGGCTATGAGCTATACAAATCTGAAGCAAGATGAAAACTCAACAGAATCATAACTTTAGAA +GTTTTTAAGTACTGGAGAGCGTGAGGAGCGACAAGCTCCTATAGATGATATTCTTGTTTAGCTGCTATTT +GAAAAGGTTGATCTGAATGTCTCCCGGTTCACTTCTAACTTAGTGAGAATTTGAATAAAGAGTAACCTCG +GATTCTACAAAATCAGGGAGCTTTAGAGCATTTTGCAAGCTCATCACAATCTTATTTGCACTCTAAAAGA +ACACACTGGAAGCACTCAACCGTGCGCAACCTGTCTGAAACCAAAAAAATAGGTGAAGTTTTATTAGAAT +CACATGAATCTTATGACCTATATAGCCTAAGCCAATTAGAGTTGAAAGAAAGGTTGCATTTAAGTGAAGA +AAAATCCTGCCCACTAACCAAATCTTATATTTCAGTCAACTGAGGGGAGCAAAAAGTAGCTAACTACATT +TTTTGTTTCCCTTTCGAGAAAGTAACAGCTCAGCCTTGTTCTACATGAAGGTCAATATCAGCCTCTTATC +TCGAAGAAGAGGAAAGATCTAGATGTAGATGATGACGCGTGCTATCCATGCCCTCGTGAAGACCACTCAA +GTTCAAGATTTTGAAATCTTTTTTTGGCACTCCCCGCCAATGAACTCACCAACTCAGAACATACGGAATT +TATATATGAATAAATGGAAGTATACATTGCAATCATAAAATTTCTAAAGTAGGCTCAAAGTCTGTAAAGT +GATAGTTCGTGTTATATATAGGTAGATTGATATTGATCAGAAATAATTTATGCATACGTTTAAACCCCTT +CATCTGTTTAAGCTAATAGTCCTTTTGGTTTCCAGAAAGACATGCATGGTACAGCATTTCTGTGGAAATT +TGAAATAGCTTATTATGTATAATCATTTTATGGTATCTGAACCGGTACCCTGCTTTAAGACTTTTTGCTG +ATGCTTTTATTATGAACTAACTATGTAGCATTCCCAAAGCCATCTAATATCCAGTATCCAAGTATCCAGA +TGGCATCCATGTTGAGGTTCGGAGGTTCGATCCTTGGGTGATCATAGACATTATTGATAGTGCGCAGAGA +CAATGCTTGACGGCGAGCACCTTGAAGATGCATCGATTATTGAAATTAAATGATGGGATTGTTTCTGATG +CGCTTTTTCCGAAACATAAGGTTTAATTCTAAGTGAATTAGATGTAAACGCTTATGATACATGTATTCTT +CATGTAATTTTATAAGCACGTAGACCATAATTTCCTGAATAGTAAATTACCCTTTTAGGAAAGCACCTGC +ATACCTGGATACTGAGTATAACCCAGGTTATTATTATTTCCCTTGAGGGAGTATCTCTATATATACTGAG +CTTTAGAAGAGCTCCACCTAAGCCACATGTGATTCTACTAACAGTATCAAATTTTGATACGTTGTTGACG +AATCGCATACCCATCTCAATTTCAAGCCAATAGAAAAATATTCCGTCGGATTAACCGGATTTCAAGAATT +CTAAAACGCAATATGGAAATGTAATCTGATTACATGAATAAGAAAGCAAGAAATAAGCCATACCCTCATA +AGGGAAATGAAGTTTATATGGTACCTCCTATAATAAGTATATAAAGAGATTCTTCTCATAATCGAATACA +CTGTATAAAATATGCATTAGTAAAAGTCTTGAACCGCCAGATACCTCCCTGCCATAACCACTACTGTGCC +TCATTGAGTTTAAGTTATTTCAGATTTCCATATAGTGCCTAGGAGTGAAGTAAATATTGCATTTCTGTGA +CACTCTGCACGTTTGTTTGTACGGTCGGCATAACGAGTCTATCTTGATAGGCGTTGAAGAACTCACTACA +TAGCGTTTTTTAGAAAAGCCTGGGGCTACTATATGTGCAAGGCCCATGATATGGAAATTTTCGCAACTAC +GGTTGATTTAATTCATTCAGGTTGGGGAATATTTGAGGTAGTTTTCGGATTAGTTGTTTGAGAGCTTGTT +TTGTTTTTGTTTTGACAACCAAGAACAAGCTGCTACCTGTTCGTGAATCATAGCCTATTGAATTTGATGG +ATGCAAGTGTCGGTTGTATGTTCACCAAGAAACTATTTTGTAGAAAGCCGGGGTTCATGGGAAACACTCA +GTTAAGTCATTCTGTCGTTATCAATAGTAGTATCGATATTTTCGGTTAATGGGGTCCTAATTTCTATCTA +AAAGATAATGAAGTCCAGGATTCTGTTAGAACACAGTCTCCTGAAAATCCCCGATTTGGAAAGTGAAAGC +AAACACGTCAAAGCTCCTTCTTTACAGTAGACGAAACAGCCACAAATCAAGAAAGGGGTGCTATAGTTGT +GTTTTAGCGGTCCAAGGATAATCATAGGGTGCACTATCTTTGAGTCAGTGTCCAGAGTGTTTTATCGCTA +ACTGTATTCTGTATGTTTTCAGTGACGCCACAACATAAGCAACAAAAGAGAGCTTCATTGTTGAGTTTAC +TCTGCATTGACTAAATAAAAAGGAGCCTCCCTCTTATAACTTCCTCAGAACTTTCGAGAAGTACAGCTTC +TCATGAGCTATAGTGTTTAATGTAACTTTTCAATGTAATGAAGGGGTGCTCGCACTTAAACTCCTGCGAA +ACTGGAAGAGTTACCTACCATTTGATCATTTATCGTTTTATATTCATGTAATATAGCTATTACATGTGTA +ATAAGTTATTTCATTCTTCTTCGCTACTGTCCCCGTCTCTGTAGCCATACTTTCTAATTTCTCCCAATCT +TTTTCTTGCTGCGATTAGGGCATTCTCCAATTTCAGAATCTCAACTTGTTGCTCCATCTCCACAGTTTTG +TTTTCATGATTTGTTAGTTTTGAATAATCAACATCATTCTCACCACTACCGCCGTTGCTTCTCTGAGTTA +AAATGTCTTGTACTTGATTAACTAGACTTCTGCAGGCAGATGTGACGTTCTTGGACGCAGTTTCCAAATT +GTCTTGAGTCTTAGACAAAAACGTAGCTTTGACTCTCGAAGCAGCTACCAACTGAACAGTTGAAGCAGCC +ACCTCATGTGATGCGACAATTAACTGCTCATGAGATTTATTCCCAGACAGAACACCATCCGCAGTTTCGA +TCAAAATCTTAGTGGAAAGGGCAACAGATTTAGCTGCTGAGATAAGGCCTTCTGTCCAACGGTTGTGCTT +TTTGTAGTATTCAGTCTTAGAAATTGAGCCTTTGTTCTTTGCAACAATTTCCTCTTGTGAAGCAATAGCT +GCTTTAATCAAAAGTTCAATGGAATTGGTAACGGCAATCGCAGCGGAAACAATAGCTTTGTTAATCTCAA +AGTCGATTTGTGAGATAGATGGGTCTTGATCTTTCGAGAGTAGCGAAGTAAGCTGGGATGATGCCTTTGA +AATGGAGGCGGCCGCATTCTGCAATTCAGTTTCCACAAGGTCGCCCAAGTTCGCAACGTTGCTGTTCAAA +AGATTAGATCTTGGGGCTAAGTCCTCAGTCAATTGGGTAAGATCTTGAAGTTTCTCTTGCACTGCCAAAT +TTCCGGCCATGACCACATCAGTCTTCTCTTCCACTTCGCCCTCTAAACTATCGGAAAGCAAAGATTTGAA +GAATTCTTCCGATTCCTCTGCAGTAGCTCTCGCAGTAGCTATGAATTGATCAGCCTCTGCATCTGATTTA +ACAAGGCGCACAATACCCTTAGAGTTTAATAAAACGTCAGCAATGGAAGAAGTGAAGTCATTGAGAGAAC +GGATAATCTCAGCGTGATCTCCATTTGGCCCATCAATGATGAAGTCATTAAAAGAGTCACCGAAGTCCAT +AGCAGAGGATGACGCTTTCTCGATAATGGAGAGTAAATATTCAGGCGATGAGTTCTGGTTACCAGCTTGC +ATAGGAGAGTCCAATTCATATAAGGCGTCTTGTAATCTCTTGAGGCCACTGAGCAAGATAGCATCAACCA +AGGAAGTAAGCTTCGTTAAGTGATCAACCAAAACTTGGTCGATCTGCTCTTCAATAGCTGTTTCATTCTC +CTTCTGAGCTTTGGCTAATTCCACCAGCGCATCGTCCATTGTTTGTTGGAGAATTTCTAACTCTTCATCC +TTATCCTTTAGTAATTGTTCCAATTCTGGATTGAAAGCCGAAGATTGCTGTCTTAACTGGTTCTCCAAAG +CTTCGATCTCTTTCTTGTGTTGAGCAAAAATAGTGGATAGGTTCGATGATTGGTTTCTCTCGGCATCATC +TAATTTCAAGGTGAGATCTCTAATTTGCAACTCAAGCTTGTCAATCTCATCACCTTTACCACCCTTAACT +TTCTCCAAGTCCAAACGAGCACGGTCACGTTCACGGATTAGACCAGCCAATTCCACGTTCTTCGATTTCA +GGTCCTTTTCCAATTTCTCACGCTTGTCAATGGCTTCTTGAGCAGATGCGGCTTTTTGTTGGAGTTTCTT +GAACTTATTTAGCAAGTTCAAGTGTTCCTGTCTCAATTGAGAGTAGAGCTTAGCTAAACTCTCATACTTG +TTCTTCCATAGAGTTATTTGCTCATGCAAGTTTTGAATCAACTCATCCTTGGAGCCAATTTGCTGAGTAG +CTGATTGAGTGGTAGCTGTAATTTCAGTCTCCAAGGCCTTGACACGCTGATCATATTGCTGTAGTAGAAG +TTGGTCTTGCTCATATTGTCCTCTCAAAGCCAGCAGATCGCGCTCTAACTCAGCTACGCGGCCCTGGGCT +TGTGACTGGTACTGCTCGCGAAGCAATTGCTCCTGTGCAATACGCTGCTGTTCTTGCTGTTGGACCTGCT +GCTGTTCGAACAAAATTCTTTGTTGTTCAGCATACTGTTGTTGTTGGAGAGCTTGTGCATCACGCTCTTG +TTGCAAACGAATTTGCTCAGCCTCAAAACTAGATTGCTGATTGCTCCAAAAATCGACACCAGTTGGTTGT +GCAGCAATTGGTTGCTGGAACTGGGAGACAGTTGGCTGCTCAGAAATTGGTTCTGACAAATTTGTCTGAC +GAGATTCTGGTCTTGGAGTCTCCTCTTCGACGGATGATTCATCCTTAACCATGAGGTTAGGAGCTGTCTC +TGGTAATCTAGGAATTGTGATCAAAGAAGTCAAGTAGCGAATGGAGGAGCAATCAGCATAAAATTCGAAC +AAACGAGCATGTTGCGACTCGTAGCGCTGCTTCAATGGCTCTAAAGCATCATCAGATCCTGTGGAACGGT +AAATTGCCCTCAACATCGAGGTGATGAACTTATAGATTCCATAAGATTCAGTAACAAGAGGGACCAATGC +TGAAATTTTGCATTCAGATCTCTTATCATGTGAAATTGTTGCGAAAATAAGTCTCTGCAGATCGTCAAGT +GAGTCTTGAAGGGACATCAAATCCATGATGGCTTCATAACCTTCATCTGGGTTTTGGACAGTCACCAACG +AGATATACTCTTCGTACTCAAAGGTTCCATTGAAACCACGGTGATTCCGATGAAACTGAAGCTTTCTGAT +CAAGTAGGTGACATATTCTTTAATCAATCTACCATAGCCCTTGAATCCATCACCTTGTATAGATCTCCCT +AAACCATCGATCCAGTTCAAGTTCCTGTAACCTTCTGCGAGAGCAGATTGGTGACCTTCTTGAAGAACTT +TGTGGATGGTGATTAGAGCCTTAAATAACTGAACTTCATCATTCTGAATAGGCAAAATCTTTAAAGCGTT +CCAGAACGAGAGTGACGACTTGTGATCCCAGGTGTAAACAATACACGCTCTCACATGCTTTCTTTTTGGG +GCAGTCTCATCAGCAGACGTAGCCTTACGAAGGGCAGTCTGGAGATCAGACTCAGAGATCGTCTTGGACA +TCACAATGGTTGTTGGCTAAGAGAATATTTGTCGTAGTTGTTCGCAAAATAGAACCTCCAATCGTGCCAA +AGTTGACGGACAATTCGGTCGTTCTCAATTTCGGCAATCTTGACCCTCGGAACTTAACACCTGCACACCA +ATATCGAAGGAGGAAAATTACGCGCGAACTCAAGGCTAGTCCAAAAAATTCACGCATTTAGCAATTTCGC +GAATCAAAGAAAAATTTAAGAAAAATTTAACAGTGTGAGTTATAGAGAAGGACGCTAGACTATTGCATCC +ACACGGTCCATTTCCTGAGCGAAAACGTATATATTGTCACTGATTGGACACAGCCAATTGTTAGACAAAC +CTTATTTCGTTAAGGGATCATCTTTTTTTGGTGCAAGGAGCTATAAATACTCATTGTTTTGGCCTAGATC +ATGAGTGGAGCATTAAAGAGAAAGTCAGAGCTATCTGACGAGCTGAAGTCTTCCAAGAAGCCATCGTTAA +TCAAGACAAAGCTTAGTGATGACATCCCGAAGACTGCCGACGAGCATGAGAGTGACGGTGAGAATAATGG +AGGTGATGACCACGAAGTTGACGAAGAAGAAGTTGATGTCGAGGAACAACAAAAGTCCTCCTCTTCAAAG +CCTCATTCGAAGCTTTCATCTCAAGATATACAGATAGCGAGAGAGACCGCAGAGCTCTTCAAATCCAATA +TCTTCAAGCTACAAATTGATGAACTGATCAAGGAAGTCAAATTGAAAGACCACAAGATTGCCAAGATTGA +AAAGTTTCTACACAAGCTTTATGAACTGATTCAAGATGTACCTGAACTTCAGGACCAGCCACTAGATCAA +GTAGAGTCATGGTTCAAGAACAAGAAAACTACAGTGCCATTTAATGACCCCAAACCAACTAATGTGAATT +ACAAGTTTGACTACAAGAAGCCGGAGAATGTTTCTATCGTGGGGTCATTCGGCCTCAAAACAGCTATCCA +ATCTCCACATGGCCTAGCAATTGACGTCAATGTTACTATGCCTACTGAATCATTCCAGGCCAAGGATTAT +CTGAACTATAGGGCCTTGCATAAGCGTTCATTCTACCTCGGATACCTCACAGAGAACTTGAGAGTGATTT +TCAAGAAAGAGGGTCTCGACTTTTTGAAACTATCCTACACGTACCTCAACGGAGACATTCTTACACCAGT +ACTGAGACTGACGGCTACAGATTCTCAGCTTGATTCAGAGTACAACTTCTACAAGACTAAATTCTCCATC +AACGTCCTGGTGGGCTTCCCATACAGAGTATTTGACCCTAAAAAGCTGCTACCAAATAAGAACTGTATTA +GAGTCCAGAAAGAGGAAGACTTGGAACTACCTCCAACCCCACTTTATAACTCTTCCATCCTCACGATGAC +ATCTTATGACTACTTCTTGAAATACCTATATAAAGCCAAGAAATCCGCAGAGCAGTTCAAAGAAGCATGT +ATTTTAGGAAGGCTATGGCTATCGCAAAGAGGTCTGGGATCGAGTGTTGCACAAGGAGGATTTGGTCATT +TTGAATTTGCCACGTTGACTGCTGCTCTCCTCAATGGTGGAGGTGCTCAGGGAAACAAAATCTTGCTGCA +TGGGTTTTCCTCTTACCAGCTGTTTAAAGGTGTTATCAAATACTTGGCTACTCAGGATTTATCTTCCGCT +GGCTACCTGCAGTTTTACTCCGACTCTTCAGATCTCAGCAGATATATCTCTGAGGGGTTCAATACTCCAA +CCATTTTTGACAAATCATCCAAGATTAATATTTTGCACAAAGTCACTAGAAACTCATATGCACTGCTAGT +GCAACATGCAAAAAAGACGTTGGATCTATTGAATGATGTGATTCAGGATAGATTTGATCCGTTGTTTTTG +AAGAATGCCAATGTTGATCACTTGAAATACGATTACAACATACAGCTCCAAATTGACGCATCTACTGACG +ATTCATTTGGTCCATTCGAAAAGATTACCCACCTAACATATGAGAACTTCATTACCAACAAAATTTCACA +ACTGTTGAAATATGGTTTGGGTGAGAGAGTAAAGGGCTTCAATGTAATAATCAAGAATTTAGTGACAGAG +TTCGAAATTTCCAAGCGTAAACCTTCACAGCAGAGCAAGGTGAATGTTCAGATTGGTCTGTTGGCTGACT +CCCACGAATGTGAGAAACTTGTCACCAAGGGTCCTTTGGAATCAGAAGATAGTGAGCAGTTTAGATCTTT +TTGGGGCTCCAAATGCTCTTTGAGAAGATTTAGAGATGGTTCCATCTCAAACTCTGTTGTGTGGACAACT +GATTACAAGCAACCAATTATCCTTTCGATTGTCGAATATGTCTTTAAAAGGCATTTGACCGAGGAAATCA +AGATTGAAAGTTCTGTTTATGACTTTAATGATTTGTTGCCACTGCCAAATCTTCCATCATCAACTAAGCA +GTCTATCACTTCTACCAGTGCTTCTGTTGCTCTTCGCAAAAGTTTTGATGAGCTCTACAGAATATTAATT +AAATTGGAACTCCCATTAGCTGTCAAGTCAGTGCTACCTGCTGGCCCAGCTTTGAGATCTACTGCACTTG +TGACTCCTGTCCCATTTGCTGTTTCATCCAAGGAATTTTTCAATGATTTGATTATTCAGTTCGAATCATC +AGTGAAGTGGCCTGATGAGATCAGTGCATTGGAGAATACTAAGACTGCGTTCTTGATAAAGATTCATGAG +ATTTTGACCAAGGAAACTGTTTACAGAAGCTTTATCAACAAAGACGAGGACTCCATTCCCTACAACTTCG +ACATTACAACGTTGAACGTGTTGTCTCCAGAAGGCTATGGGTTCAGATTGAGAGTATTGACTGAGCGTGA +CGAAGTGTTGTATCTGAGAGCGATCGAAAGTGCTAGAAAAGAGAAAAGATCAGAATTGGAATCTATCTAT +TTGAAGTTCAACCAAAAGTATCTTGGTTCCATTACTCATACCAGAACGATCTCTACTGTGTCACACAAAT +TCCCATTCTATTCACCAACAGTAAGGCTTTTCAAGAAGTGGCTTGACGATCAGCTGCTTCTACCACACTT +GACTGAAGAGTTGGTAGAGTTAATTGCATTGAAGCCTTTTGTTGACCCAGCACAGTTTGACTCACCTTCA +TCAGTGGAGAATGGGTTCTTGAAGATCTTGAACTTTCTTGCCAGTTGGAACTGGAAAGAAGAGCCCCTAA +TCTTGGATTTGAGTGCCAAGCAAGACGACATTGAAGACGCTAGCGAGATAGTTTCTAAGCTTTCTGATAA +GATTACTGTTCAGTCATATCAGCAGATCAAGTCTAACTTCGACAACATCAGAAAGCAGGATCCGTTGGGC +ATGAGAACGCAATTCTTTGTAGCTTCGAAAATTGACAAGTCTGGTATTTTGTGGTCTAACAGTGTTCCGC +TACCAATTGCAACCAGATTAACTGCTTTGTCCAAGGTTGCTATGCAGTTGGTTACTAACAATGGCGTGAA +AAATGATACAATCAAGCTGCTCTTTACACCGTCTCTTGCTGACTATGATTTTGTCTTGAAGTTGAAGTTC +ACTCCACTCACTCTGAGCTCAGGTGTCTTGCCAAAAGGAAAGAGGACCTTCAAGAACTTGGTTAACAACG +CCTCATCATATCCTACTGAGCTTCACTCCAAGTATGATCCAGTGGCGCAACTGATTAAACAACTCTGCTT +GAAGTTCAAGGGTTCAGTAATTTTCTCTTACAAACAGAACAACATCGATGAAAGTGGAGAAAGTGTGATT +TGTGGTTTGTTTGTTCCACACACTTTACAAAAGACAAAGTTCAGAGTGCAGATTGGTTACAACTTTGAGC +CCCTCGAAAAGGACGTTGTTATCATCAACAAGGAGGCCATTTTCAATGAGATTTTGAACTTTGCTGGAGA +TTTAGTTGTTGGGTTTGAGAAGAAATAGTTAATGAAAGTTATGTATTAATCTTGAACCACAGAGTATGAT +TTGTTATATATAAGAAGCATACTTAGCTACAACTGTAGTCGGTGGATTTAGGACAAGAACACGTCCTAAA +CCCAAAGATTTTTTTAACTCGGTATTCCGAGGGCATGCCATGGCGATCAGATCATCGAACTGTCATGAGG +AGTTTTTATTGTTTAACAATGCGTTCTGTAAGAATGGTGGAAGTTTCATAGACCACTTTCTTCTTTCATT +TATATCTACTGATATCGACGGCAAATGATCAGGACAAGAGCTTTGGTGCTGAGAAGACAGTGGACTGCAC +GATTGCCAGTCCCGCATCTATTCCATTCTGTGTGTCCAAGACCTAACAAAGAGCCAACAAAGGTCATTCT +CAAGTTGAAGAGCAATAGACAGATTTATCCAGATGCAAAGAAGGAATGGGAGCGTAAGCTCTACCTTGAC +TCTTTACCCACACTGATGCGCAATGTTCGTGAAACAATGGACAGGTACAAGGGCTACATCGTCATTACGC +AGGTTGGCAGTTTCTATGAACTCTACTTTGAACATGCTACTGAAATTGCTCCAAGGCTGAACATCACACT +CACTAAGAAGACATTCAAGGAATTTACTGTACCAATGGCTGGATTTCCCCTGTCTCAACTGGATAGACAC +CTTAAGGTGCTTGTAAAGGATTTGAACATGGGCGTCGCAGTTTCAGAACAGTTTCAGAGGGAAACCTCAA +TTGATAACGACCCAGTGAAGATTGCGAGACGTGTTACAAGAGTGATCTCTCCAGGTACGCTTGTTGATGA +GGCTTTTTTGAACGAGCAGGAAAACAACTATCTGTTGGTTATTGATATTCCCAAGAATTCCGTTAACAGG +CCAGCAGATCCAGACATAGCTGTTGGGCTAGCATGGATTGATATATCTGTTGGAACATTGTTTGTGCAAG +AAGTTACACTTGAAGGTCTAGCGTCAGCCATTTCGAGGATAAAGCCTTCTGAAATTCTGTTGAGTGAAGA +TTTAGTTGAGTACAGGCTTGAAAGCGGAGATTGGTACCAGGAACTTATAGAATTGAATAAGTATTTTGTA +AAGTACCAGAACTTACCAGCGCGACACAAGACGTTGGACCTATTCTATGGCATGTTTAACTTCACCCAAT +CTGTCATCAAAAAGTCATTTGATATTCTTTCAGTAAAAGAGGCTTCCGCAATGAAAAATTTGTTGAATTA +CATCGAGGAACACTTCCCTGAAACGAAAATAGGTCTTCAGTTACCAGAGAAACAGTATCCTGAGATGATT +ATGCAAATTGATTCCAGAACATCAGATGCCTTAGAGTTACACAAGACATACAGGGACCAGTTGGCGAAGG +GTTCTCTTTTGTCTTGTGTCAGGAGGACATCCACACAAGGTGGTGCTCGACTCCTTTCCCAATGGCTAAT +GGCACCTTCGACAAATGTCAATGAAATCTTGTCTAGACAGAAAATGGTTACAGTATTTTTTGAAAAGCCA +ATTTTTAGAAAGCAGATTGTAATGAGATTGAAGGAATGTCACGATATCAGCAGAATCGTTCAGAGATTTT +CAATGGGAAGGGGTAGTATTCTTGACCTTGTTCAACTCGCTACAACAGTCCAGACCTTTGAATGTATCAA +GGAAATTATCAATGAGAAACTTACCAATGATGACAATGCTATCAAGTCACTCCCACCAATACCAGAATCG +GAAATGAAGTTAGCTAAAGAGATTCTCAAGGATATCGATGTGGAACATCTACTCACTGATCTGGAAAATC +AACAGAAAGTTGCTAAAGAGGAATTAGAATCTCTAGCTAGCGAAACCTACAGCTACTCAACTGCCAATAG +AACAACTGCAAAAACTTCGCCACCCCTGTATGTTATCAGAAAGGAATCCTCCAAAACTTTGAAGTCACTC +CATGATCAAGTCGATGCGTTACAGCAGCAACGTCTCGATCTCGAGATTATGTTGAGGGACAAATTGAAGA +CTGAAGGTGTATTTAAAGAGGTGTCATTGAAGCTGAGTCCAAGTTTGGGATATCAAGTATACATCAAAGG +AACAAAGACAAGTAAATACGATGACCTTAGCAGATTGTTTCCCGAGGGTGTATTGAGCCAAAAGAGTATG +TATACAAGATGGTACAGTTACCCAAAGTTGACATCATTGGGGCATGCTATTGATGCGATGAGGGTTAGAA +TTGGAAAGGAGGAGAATAATGTTGTGAAATCGCTGATAGCAAAAGTCATCAAGAGAAGCATCCATTTAAG +AAACATTGGGAAGTTTCTTGACTCACTTGATGTGACTAGTTCTTTTGCCATTTTGGCACATGAGAGGGGG +CTTGTGTGCCCCGAAGTTAATGGAACGACAGATCTTGAAATTGTTGATGGGCGACATCTTGCTGTTGAAG +ATTCGTTGAATGCTGAGATGAAGCGTTTCACTTCAAACGATCTGACACTTCACTCGGACCAGTTTGCATG +GATCATCACTGGTCCAAATATGGGTGGGAAATCGACATTTTTGAGACAGAATGCCATTATTGTTATTCTA +GCACAGATTGGGTCGTATATCCCAGCCACTCGTGCCACGATTGGTGTTGTAGATAAGATGTTCAGTCGCG +TGAATTCTACGGATGACTTATCTAACAATATGAGCACATTTATGGTTGAGATGGTAGAGACAAGTTACAT +ATTGAATGGTGCCACAGATAGATCGTTGGCCATTCTGGATGAAATTGGAAGAGGTACAAGTTCCCGAGAG +GGAACAGCAGTGGCATTTGCTACACTTAACCACCTGGTTGATCAAAATAAGTCGAGAATCCTATTCTCTA +CTCATTACGGTAATGAGATTAGCCGTTTAATCAAGAAGTATCAAACTACCGGAATTGAGACCTTCGCATT +TAAGAAAATTACAGCTGAACGAGTGAGCGGACATCTTCATTTCAATAGAAAATTAGAAGATGGTATTTCC +GAACAGTCATATGCTATTGAGGTGGCGGAGAAAGCTGGTTTCCCACCTGATGCTATTGCAGTTGCCAGAG +AAGTGATGAATTTGAAGATCGAGAATTCACATTGATAAAATTCTCGTCACCTCAAGATAATAAATCCACG +CACAATTTGCTGCAGATTCACGTATCAAAACAGCTTTTTATTTTTGTAGCTGCATCGACGTCTAGGCCTT +GTAACACAGCCACGCAAAGAATCCCACAAAAAATCAATACACACACCATACCACACTTGAGTATGGCATT +CAATAATTAGATAATGAGTAATTTAGCCCTGGGTTAACGCACTTAAACACTAAAGTTCCACTCGTGAGCC +TCTTTCTCTCCCCACAGGGGTTGTCCCTATATTGATAGCTACTGAGCAGGATATTCGAGACCCCAGTGGG +GGTAGGGTGGGGCTACAGTATTGTGTACAAGAAAAGGCAGTGCTGTGAAATCAGTGCCATTCAAACAAGT +TGAAAAAATATTGTTTGCCCAGTCTTCTTTGAATTCGCACATAGCAATAGCAATGATTGTTTTTGAAAGA +ATGTGGTTAAATTAGATCTCCACGTTGCTTCAAAAAGACACGATTATGCAGGTGTTCTATCCCTTTTAAT +AAAAATGACGTCATCGTCAGAAAGAAATGGAAAAGCGAACAAAAAAGAATAAAAAAGAAAAAATCATTCC +GTCATATCCGTGTCTCGGATGGATTGTGGTAATTGCTCACTGAGTACTCAGTACTACCTGCTAAAGATAG +AATGAAAATACTTTGAGAAGCTTTTAAAGTTGTTCCATTGCCAAAAAGTCCATAGTTTATTTCCCGATTA +GGTATCAAATGCCGCGAGACGTGCATATCTGACGAATGCAGGTTCCAGGAACAGATAAATTGCTCCAATA +AGCTGTGAGTTGAGAAAGTCGTCATTGTCGCATTGGTCTGTTAGAATTGCAAAAATTCAGTTCTCTTATG +GGTAAAAAAGATACTGTGGTTGTTTTAATTAGGAGGTGAAGTTCCTGCACAGAGACATTGCTTTTCAAAA +TTTTGCACAGGTGCAACTTTAATCAGTCACGTCCAGTGGGGCCTCAAGATTGCGCTGCCCTAATTGAGCA +GAGAAATTAAATTAAGCCCTTCGTACAGATTCCAAGATTTAATTAAACTTATGTTATGGCCACATTGATT +GGCAATCTGAGAAGTTTTCCCTGTGGGTATAAATTGTAGATAAAATGTCTTCATATCTTCTCTTTTTTAC +ATACCACCACATAGACACAAACACTAGCCGATAATGAAGTTCTCTACTGTTACTGCCATTGCTGCCATTG +TTGCTACTGCTGCTGCCCAAAATAACTCTAATGAAACCTCCTCTAATGCGGCCGTTGTTGGCTCTGCCCC +AGCCTATGGTGCTGCCGTCGGTGCCATTGCTGTTGGTGCCGCACTCTTGATTTAGACTACAGTCTGTTGT +TGAAAGTACATTGTTGATCGATAAAGCTTTTGTATTGTTATGGTTAATGTCTAATTAAGGCGAGGTTGAT +TGTAGTTTGTTCAATTAAAATCTCTAGGGGTTTTTTGACGCTTTCCACCGCGAGAAATTTCATTCTTTTG +TTGCGATGGGGCTGTGCTGTGTCCAACAACAATTGCTGGCAAATTTTCTAGCATAAGTTGATGCGGATTT +CATAACATAGCTGCGAGCTTATCTCCTTCTCCTCCGTAATTCTTCAGAAACGACTGTTCTATTGTGGCGA +AGAGCACAATCTCATTTACCACGGACAAAATCAATGCAGTCAGGATAGGACTGCGGAAGGTGGGAAGGTC +TGTAATGTATGACTGTCAACCTCTGTCAACCTGTTCAATCTTTGGTCTGTAGTCTGTGAACACAGTAGTG +ATGTTGATGAGCATATCTGCATCATTTTGGGAGCATGGGCTGCCTAAAAGATGTTCACGTATAACATAAA +AAAAACTGGTTTGCTTGATCAGCATGGTCTCTCTGCCTCGATGAGTTTTCAGGATTGTCTTACAGTCTCA +GACTCCAGACTTCAGTATCCTTGTACAGAAATGAAAGACAATTAATTGCCTTTTCAGTTTTATATGTCTA +TTGCATAGGCTTGACCAATGTCTAGCGAGAAGGTGCTCTGTTGACAAAAAAATGGGCAACTGAAGACAAG +TTACTGCCCTTGATAAGCATAATTGACGCTCTAATATCTCAATGTAGTAATAGAGTTACCTACACCCAGA +GGAGTGCCACTATGACTTTCTTGATGCCCATTTTCAAAGTTGACACAATTTTTTAGTAAGAAGAAGCTTG +AAAAAAAAAGTTTCACTGTCAGAAATCTAAAATCAATCACATTTGTAAGAACTCCACCACTGTCAGAGCT +TCAAATCTTATCCGCCATGAGTGATCTTCTAGCGAGTGACTCGCGACCACCATTGTTCACTCTCAGAAAG +CACACTCACCCAATCACGTCCCTCCAATTCATCAAAACACCATTAGTGACATTCAAACAGATTACAGCAG +CTAAGACTCAGGCAATCCAATTGAAACCATACCTAGTGTCTGTTGACGAATCAGGAGTACTGATTGTTTG +GGATCTTTCAACGCATAGGCCGGTACTACAATTCCAAGCACATTCATCTCAAATCATGTCTGTGCGACAG +ATGGGAGTTGTAGATGACATAGTTGATTGGAGTTACTTTGGCCTGGTGCTGACACATGGAAGAGATCACT +GTATCAAGTTCTGGAGATTATTTGGACCTGATGGTGTTATGAAGGAAGAGATGGTGTATGAGCTTCCCGT +CAATGCCTTGAATTTCAGTAATGTTGATATCTTTGACCAATTGTTAGTAACTCCTAATACAAGAGATTCT +GAGGCATTCGATGTTTATGATGTGTCATTTCTACGAAGTGATGGCGAGTTGGATGATCGAAGACTGAGGA +GAGTGCTTGAATCTGTGGATATCTTTAAACAGATTAAAGGTTCTGAATTTGATGAGATTAGAGCCAGCGG +AGACGACGATGGAGATGTAAATAGGAGCGGGAAATTTGGAATCATTATGAAGTTGCTTTGGACCGTTGAG +AACATATTGTTCATTGGATATGAGTCTGGACACGTTGCACAGGTTCAACTAAACTTAAGCTCCCAATCAA +TCAAAGTTGTTTCTGTCGCTACACACCATTTTCCTGACCCTGTTCTATCGTTGACATTTGATGTAACAAA +CAACACAGTGATATCAACATCGATTAAACCAGAAATTGCGCTGCACTCCATTGGATCTACACGCTCATCA +TTGATTACACTCGACAAGGGATCAGGATCAGGAAAAATTGCTAGTGTCGGATTCATTGACGACAGATATG +TATTTGCATCTTGGAATGGAGCTATAAAGTTCTATAAACTCAATAAGGAGTTATTGGAGTTGGAATATGA +GTCAAGTTTTAGGAAACCAAAGGGTATGATAACTGGTAATTTGAACATAGTTGGGAACTTGAATGAAAAT +GACGATGTTAAGACAAAGAATGCGAAGATCACGAAACCAAATGCACTGTCAATTACAAAGGGATCTGAGT +TGAGAAGGGACATCGTATTAAGGAATAGAAGGGATGCTAAAGTGATTGAGAATGATTTCATGGCTGTTGG +GTACAGTGATGGGACAGTAATGATGTATACATCTTCTTGATTGGCATTAATTTGACAAGCTTCTATGAAC +TGCCCAAACTCTTCCAGTTGGTAAGGAGTACAGAGTTAAGGGCGAGAGACTGATAGTATTCATCTCAAAA +AAGAGACATACAATTGATAGAATAAAGACGTTTTGCATACATAAGTTCAAAATATTTGCTGAGTACGTGT +TTTAAGTGATGAAAGTGGAGCCTTGAGGATAATATTTGACAATGTCTCATTCTCTGCCACAGATGGAGCA +GCATAAGCTAATGAATTATCGGAGGAGGTTGTATTTCTATTCTTCAAATTTATTTTCAGCAATGAGCGTG +CTGGCTATTCTGGATACTCATTTTTCAATAGACGGTAGACGATTTTGGCACATTTGTAGGCCCAGGGATG +CTTTTTCAAAGTACAGGAGTAGTTGTACCAACATAAGAATCAAGCACGACTGCTCAAAAGGTGAAAACTA +TGTCTGAAGCAAGCTAGTCGGCTTCCATGAGCTTTATCGAAAAAAGTTCAAGAATATCTTCATGCAGAGA +AGGTTTTATTGACAATGAAGATCTCATGATCAGCCGTTTGCCTTAAAAAACCGTCATTATGTTCAAAACT +CCCGTCGAAAAAGTTACATGAGGATGACCTACAAATATACCTATGGCGTAACTTTCTTTAATATTTTCTT +TTTTCCTTTAGCTATAGACATGGGGTGCTACTATTAGAGGGATTCACAAACGATCATCCTCAGCCTCTGT +CAAGTCACCATCGCTCAGCAAATCGGTGTCATCATGCTGGGTAGTATGCAAAGAGGCCTCGTCTAAGATG +TCATCAGAGTATGCAGTGGCCGTAGCAGAGCTGTAGTCGAGAGAGACACTCTTCTTAAAGAGCTTCTCCC +TGATCAACTCAATACCAGGTTCAGTCACTGTGCTGATAAAACCAACTGTTACGACAACGACATAGCCCAC +AATTGATGAGATTCTGTTGAATGTGGTGTCAGTGTCAAATTCAACTGGTTCATTGTCATCACCAAGCTTG +ATCTGGCCATAACGACCAACATAATGGTTGTAGATAACATAGATTAAAACACCAGCACCAATAATTGGAA +TCAAAATGACTAGAGCCAATGAGAAACCTCTCAATCCGTGCTTTCTGTTGAACTCTTCCTCACGACCAGG +GCATGGCCTAGCCTCAACCTTGTCAAACTCCTGACCACCGGAACATGTTGACAATGGAATCTTTCTGTAA +CCAGTTGGCAACCAGTACTCATCACCGCCAAATTCTTTACAGACAGCAGAGGTGTCTGGCGGAGTGTAGC +CTGGAACTAACTTACAATTACCCGCAGAGTCCCTGTAATAGTTAAAATCACATTCGAAATCTTGACGTGT +GCATGGACAGTTTCTCATCTCTTCATATGGCTTGAGCAACTTCTCACCGATGTAACAGTCGGCACCTGGC +ACCTTTCTGTAATATTTCGCCTCATGACCAAACAAGCAGTTGTCAGCCTGATGTGGGTGACGCGGAGTCC +ATAGATCAAAGTCATCAGTATCTGGGTGTTGCAAGTCTAGGGAACACTTTCTCTTTAGTAGTTGGGAGAA +GTCAATCTGATAGACGGTAGCTCTGTCACCTGAAGAGGCTGGTAGACGAGTGAATAGAAGGAACTTTCTC +GAATCATCCGATGGAACAGTGGAGAGGTCGTTGACCACAACCAACTCATCAGTGAACTTGTATTCATTCC +AAGTGTCACCTTCATCCAAAGAGTAGTACAAGACATTAGTGGAGTCCTGTCCGTTAACTAGGACCAAGAT +GGATCCTTGATCACCGTACTCCCATTGGTAAACACCTTTCTTCACTTCTTTCCAGGTAACACCACCATCA +CGGGTCAAGTAAGTATTACCATCGAGGTATTGGCCCAGCTCTGAACCAACATTTCCAACACCAACCATCA +TACCAATCGCAGAACCAGAGGAGAAAGTGTCTCTGTAATCAGCACGTTCAGTATAACCATGCAAGTGCAA +GGCGCATTCCTTTAGTGGCTTGCCGAAACATTTATTCTTCTTTCCTTCAGAGTCAACAGCTGGTGGTTGC +AAGAAGTCCCAGTCAGCACCGTCATTATGTGTGATTTTAGATTGCAATTTCTTTTTAGAACCTGTCTGTG +CTTCGTTTGGATTCGACACAGTGTTGATTATGGCGACACCCTCCAAGTTCTCCATCTGTTCAAAGTCAAC +AAATCCAGAGGAGTCTCTGTTGACATATTGCTGGGACATGACATAAGAAGTACCGTTAGAGTTGGACTTG +AGGATAGCACCAAACTCAGTGCCTGTTCTGGCATTGGTGGTGACGTGTAGGAAGATTGCATGCGATTGGG +CAGAAAGGACTGTGTACGCTTGTCTCTTGTCGACTCTGAAATTGGATGGGAATTCAGCATCAGCCCAAGT +CTTACCATCAACTGAGACGTGGGCTCTTAGCGAGCCATCCTCAGAAGAGGTGGCAGCAACAAGGAAGTCG +TCTGCTTGAACCATACCAATAACATTGGACAGTGGAGATGATGAGGTTTTAAAGTAATCCTCAGAGAAGA +TCAACTCCTCGGAATGGACAGTGTGCTCGTTCAACTCTCTGGTACAGACAATCAAACTTTCATGAGTTGG +TTCGGCAAGATGGCCAATGTAGTCACATGAGTTAACGTGTTCTTGCAACAATTGGAAGCGCTTACCGTAT +GATTGTGTTCTATACGTGACAGTACGACAGGCACCTGTGTGGGATGTATCACAGTCCTTCTGACCAACGT +AGATGAACCAGTTGGAGTGACGAGCGTGAAAACGAAGAGGATTGACACCAGGAATGAAGTTGGCTGGGGT +TCTGAAGGACTTCCAATTATCAGCTCTATCAGTCGAGTAGATGATCTTCTGGTTGGTGGATAGCAAATAG +ACGTGGTTTGTATTGTATGGGTTTACAAAAATGTCGAGGATCTCCTCCTCTGGAGCAATCTGTTCCCAAG +TGGAACCTTGGTCATGTGTCACATAGCCTCTGTTTAGGGAGGTTAACAGCACGATTGTCTCATCTTTCAA +GTTAGATCCAGGGACACGGTCCAAGTACACGTACTTCACAATCTTACCGCTGAATCCAAAGGCAGATGTT +CTTACTTTACCGTCATTTTTACCATCTTTAGAGTCCTTCTTATCACCTTCACGTTCATCTGGGTTTCCGT +CGTCTTTCTCGGTCTCATCGGGTTTGTCATCACGGTCATAGTCAATGATCTTACCAGCACGACGATCAGA +TGGGTCCTCTGTTACGATTAAAGCCTCCGTGATGGAGGTGCTTGTTGGCTCTGGTGCCTCAGTGGTGGAC +GCAGCAGCATCTTCCTGGATCGTAACAGCAGCCTCATCTTCGCTGTTGATAACCGCAGCAGCAACATCAG +CAGCCCTAGCCACAGCTGCGGTGGCGACAAGCAACTTCAGCAATCTCATTGTAATGGATAGTGAGAACCG +ATGGAGTCGTATGAGATTGAGTCTGAATTGAAAAGATTTCAGTTTCCACTGCTTTTTTCTTCGAATTCAG +GGTGCCAAAAAGATACGAAAGAAGTTAAATCCGAACGCCGAAGTCTCCGAATGTGGCGCGAACCGTTTAT +TGAGGGTGCTGTTATCGGGGATAGTTTTCCTACCGGAAATATCTGGCTTGGACCACATCCAGAAGAGGTG +AACGTATGTGTCAGTGTGTATTTAGGGTATTTGCCGGGTGTTCGATTGGCCACGCCAAGTTTTCTTTTCT +TTTGCAAAATTTCAGTCTAGTTTGATCGTTTCAGTATTAAGTCATTAAATACAGTGGCCTAATAATCAAC +TGGTTGAGAACCAGGTGCCGAGCCTCTGCCTTCACTTCTTCCCCCATCGCGCAAATATCTATGTTTGCTA +CTTATAGAACCTCTGTCTACCTCCCTTCTGTCATTCCCTTGTCTTCCTTCTGAGTGTCTATTTTCAAAAT +CTCTATGCCTTCTATCAGAATCATACTTCTTCTTATCAGAGTGATTATGATGAGATCTATGAGATCTATG +ATGCCCATGATCTCTACTTCTTTTAGGTGATCTCTCTCTTTGTCTCTCAACTTCCCTGAGTTTCCTCTCC +ATAGCCACTTCTCGCAGCTTTTTCATTCTCTGTTCTTGAATTCTTGCCATCGGATCCTCTCGAGATCGAT +TAATCTCCTCCATTTGGCTGAGCATTCCCTTATTTCCAGCTGCCACACGATCAAATCCTGTGGCCTTGGA +TTCGTTCTTCTTGTTCGGATTGACAATGAGATCATCAACCCTCTTTTTACCTAAAAGATAATCTTCATTT +GCATTAGAGGCCAATTTATTGCCATCTCCAACACCTCCAGCATCATACATCCAATCAACTCTATCGACAA +CTCTCTTTCCGGTTTGCTTATATTGTAATTCACGAAGCTCAGCGAGTTCCTGTTCTTTTGCTATCTCAGC +TTTGCGTTCCTGGATTCTCTTTCGTTCCGCTAGTGCCTCCTGCTCTTTCTCCCAGACTTTTTGCTGGTTC +TTCATGAGAGCAGGATTCCATGATTTCTTAAGGTTTAAGTCACCAGGAGCCATTGTATTTGGTGTTCGTT +GTGCTGTATTGTGATTTGGTTTGGTGTCAATAGTGAGAGGTATAATTTTTTTTCTTTTGCCCTTCTAAGC +GACTACCTCCTCGCCCTTGCTGATGTAATCAGTATTGTAATATACTTAGACAGACTGATTCATTCTAACT +GGTCTTATACAAAAAGAGGTGGTTCTATATACGTAGTTATAGACACGCGCAGCACTGTGGATCTTATTAT +TCCTTCTGCTCCGTAGCATGTATTCTCTTTCAGCTACTTTTTTTTTTTGCAAATGCAGGTCAGGTCAGGT +CAAGGAATACACGCAAAATTTTTCACAGCATCGCTTATCTCTGCCTGAAAATGGAAACTTGCTCATGATG +AAGGAGAGAGAAGCCCGACGAGAGATAGGCCGTGTGAAGATGCGACAGAGTAGTATCTACGAAGATACCG +ATAGCAACCAAAGTATCGATTGATGCTAATGTTGCTCATATTGAAACTAACCTATCTGGTCTTATCATCC +CCACAATTCACTGTTCAGAGCTCGCAACCACGACGTCACGGACCAAATTCCAGAGACAGACAAGATGATG +CGATGCGAATGGGAATGGATCCCCGGGTATCACCGGTCTGCTGCATCAGTCTTCGTTGGGAACGTCATCG +GGATCTGTTGTCACAGATCTTTCCCCTTTCCAGAAGCGCAAGGCGAATGCGGTGTTGTGATTGTTATCTA +TGTGGATGATAAGCCACAACGGCGGTGAAACAAAAACATTGCCAAACGCAGATAACCAGCTCCATAGTTA +AGAAAAGGTTTCACATACAAATTTTAAATGTGCTGATTAGCAGGATATCACCAGCCAATGAGAAAGGAGA +GGAGATCTGTATTTATCAGATCATGTTGATGTTTGAGATAAGGTCGTAATGGGTCGTCCCGACTCGACTA +GAGCTGCTGCTGTCAACACCTGTCAACTTCGTGTAAAAATTTAAGTTTTTGCAGTTCTACTTGAGTTTGT +ATATATCCTGCATCCATCTTCCAGTTCTTACTCTTGAGCTTCACGTGTCATGATCGAGGAGGCTTAGCTT +AGTTGTACTGCATCCTAACACATGTTTCTCTAATCTACGTGCATCTGAATAGCAATCATTTTTTCTCCTT +CTGTGATCATCCCCCCTCCAATTCGAGTGGGCGATATCAGAATGTTTGTTGGGTTTTCCTGGTTTTGAGT +TGGTATCACCCGAAGATAAGCTACATACAGCCCAGCAATCCCACAATGATTGTTTCGCTATCTCCATAAA +TGCTATATGTAGTAGTAGATTGTTTCTACCATTATCTCTATACTTACTATAGTCTATATGCCACATGTTT +TGACTTGTGCCTTGCGCACATGTCTCACTGCATGGGGCAGATCGCACATGTGCCAGTGGGCTTCTCTCGA +AAGCAACTTTATTTTAGTGGCACATGTCAAAATAAAAAAAAACTTCCCTCATTTCGAGCAGCCCACATGC +GTTGGTTGGTGAATGCACACAATAATTGGGGAACTCGTGGGTGGTAGCCCACTACGCATGCAACAGATTC +TGAGGTCATCTGCAGTTTACAAGGGCCACATGGACTAACCAGCCATAGAAGAACTAGTGAAATTTAGTAC +ACGTTCCCTACACGTTCCCTACACGTTGCCTGTGGGTGCTGATTTCAATCCCTACCAGAAAGAAAGAAAA +AATAACAACAAAAGAGGGTGTTTTGCGTAATGGTCATTCGAATCATCTTTCTCGAAGGATTACTGCACAC +AACAACAGGTCAATCAAATATCCACCCTCCTTATCGCCAGGAACATGTGTGTACAGTATAAAGCGTCATA +AGTCTAAGCATATGCGAGCCGGAGCATCTAAGTCAGTATTATAAAGTCCTGCTTTCAACAATATTCTCTA +ACAACGATTTCAATGATCCAAGGTGTAATAGTCAAGATCATAGATTCGATTCAAAAATAGCACTAATCCG +TAGCGTCTGCGGTGTCTTCTATTCTTGGATTATATTCAATCAGAAGCGAAAACAGTCAATTTACACAGTT +TCCTTTGAATTTTAAACATTCTTTCTAATGTTCAGTTGATCCCTCGACAAGTACATATTTCTACAAAGCT +TAACAAAGAAAAGGAGTGAACATCAAGAAGTGGGCAGAGGGACATTAGGCAAATAAAACAACTGTGTAAA +TGAATGGCAAAATGAAAACCCCACTCTATTCGATGCTACGGTTATAAATAAATATGTTCTGAGTAATGCA +CAGGGTTTGGAGATCACAGATCAACAAACACTCGACTTTTGTTCATTAATCACCACTCATAACCATACCA +CAGCCAAAAAACAAGACAAAAGACAAGAACAGCAACAACTAACAATGAAAATCTAACCACGTGAATTGTA +CCGAGCTGTAGATGAGATCATTAGATAGATTCAGCTATATCCTAATCACACGCAGCAAGGGTTCTTAACT +CCAGTGGACTGAGGGGAATTCAAAGAGGATGTTCTGGGCCATAACGTACATGAACAAGGCTATCTATCGG +TAATGACTTTTACACAAGTGCGTGATCCTATTTTGTGACTACCGAAATGGTAACTCAAGTTTGTTGTTTG +TAAGAAAGATCGAAAACTAACATAGGGAGGTGCGATACTTAATACTAGAAGACAGGCGAGCCCACACATA +CACGCACATACTCTTTCGTACTCTCTTAACAATACAAATCCGTGGTAGACGGATCATCACACCCCACCAT +AGAACTCTCCTAAACAACAGGTGCTCTCTCTCGATACTCAGAACATAGTGGAAATCGAAAAAAAATGTGT +CAGTAGTCTGTGTGTATGTGTGTATTTGTGTGTGCCCACCAGAGAAACGGAGTAAGAAGTTAATCCTTCA +TCGTGCTTAACGATGCCATATTGGAGAGCTGATTGATTAGCTGAATGATCACGTTATAGCAAACAAAAGT +CATTAAGGGGGCAAAACCCCATAATGGTATATACAGCCCTATCATGTTAAAAAATAAGCTGAGAGTTGTG +AAAGTTGATCTCAATTGCTCTCAACCTTTGAGCACTTTTGAGTGGGGACAAGCTGATGGAAATGGAATTG +GACGAGAGCCTAGGTGTGTATGTGTGTCAGGAACTGCATAACTATGTAGTCAGCGCAGGTTCTCGAGTAG +TGCGGACAAAGCTCCAGCTAATATGTATTATTTTGCACTCTCCTTATTGGCCAGTTGGTGCCTTCGTTTA +TGAAAACTTTTCTTCAGTTACCAACAAAACTGTAGAAGCCGTTGGAATCCCGCACGGAGTTGCAGATGAG +TCTAGGTGGGGGCTAGGTGGAGCTTCTGCCTAAAGTTGGTTAACTCCGGCAGGGATGCTCCTAAAGCGGA +ATGCCGCAACATGGATCCAACTTGCATAGATTTTACAGTTATCTGCAAAAAAAAAAGAAATTCTAGAATT +TTTTTTTTTTTGAAGGAAGCTCATCAGCAGGTTGATATAAAACAAGAAATCCCCATCAAAACAATTGGTG +ATCTTTGATATAAATAGTGTATATCACATTAGGTTCTTGGGATTTTTAACCTTCCACACCTTTATTAATT +TTTAACTATTCGAATTGATTTGCTTCGCACTTCCTTTTAATTCAATTATATCAGTGTTTAAAACTAATTC +TTGATACTTTTTGAACAGAAGGTAGCAATCTTTTCAGTACTTTCCTCCCCCCCTTTTTAAGAAATTTTTT +AAACCCTTATACCCACCCACCCTTTGTTATGGCTCCATCTACTCGTAAGTCCACTCGTGTTCGTTTTTTA +ACTTCCAAAGCTAAACATTCAGAAGAAACCTCCAAGAATTTGGTACAACCATCGATGAGCAACAACATCA +ACAACACACACACCACAAGTGTTTCCAGGAAGCCAGTGTTGAGATCTATTTCCACCGTTGATGACAATGA +GAACACATACAATAACCTCAACTTCAAGAGAAGAAAACTTCAGAACACTTCTGTCGTGCAGGCACCAGAA +GCACCTGCCGATGATGAATCTGAAGATGAATACACCCAACCAAATTTTAGAGTCCCGGATGACTTTGTCT +CCATGAGCGAGGAGGAATTCCTTGGTCAATCCGAGGACGATAACTTGTTCAATTCTGACGATGACAATGA +GTACTACAGTGCTATTTCTGACTATGAATCTGATAACTTGTTTAGCAAACCAACAGCAAACAGTCAACAG +CACAAGAGATTCATCAGCCACTCAAAGGCTTTTCTATTCAGAAAGAACCAAGACGCTGAGATTTCAAGAT +TGGCTCATGTGAAGTTCATGCAAATGGAGAATGACAATTTGTTTGAGTCTGATGAATCTGAATCTGAGAC +TGGTTCTCCAATCAACTCTCATTTCCACTTTGCTGACACTTTTGGGGAACTCAAGTCATTGGAGGAGAAT +TTCAAGAAGATTATTGACGAAAACCACAAGTCCTCTAGTCAATCCACTGTATCTTCTGAACATGAGTCGT +TAGCTGCTCCAACCCAAGCGGAATTGAAGAATGACGAGAACATCACCATTGATGACTTTGTTGATTACAC +TTCTGACGTTGTGTCTTCTGATGAAGACGCTGAAACCACCAACGTTCAAGAAGAACAACATGCGCCATTC +AAGTCTTTGTCTAAGCACTTGCACTACAGACATGATGAGAAGCACAATTTGGCTTTTCCTGATGTCTCCA +CTTTTCCTATCAGATATGACCCATCTGCCAACAACAAGAAGACCACCAAGAAGTCTGTCAGAGCCCTGAA +TAGAAGAGCTATCTTGACTGGTATGGCCAGTGAGATGGTTGGTACTGGTATGGCTATTGGTGAGTTCAAT +ATCTTTTAGAGTGCTTATATTGCTTTGCTTTGCCTTTTTTCCGTCTTTTTAGTTCAATGAAGACCCATCT +TCATTCACTTTCAATACTGTTGTTTGATCCACAACAACTGGGTCCTTTTTTTCTTTACTTCATTATGGTT +TGATTTTGATATTTGTTTTGATCGTTTTTTGTTTTAAGTTGCATTGATATCCTGAACTGTTATAGCCGGT +TGCATTTGATTAAGGGTTTATAGAGATTTGGTTTTTTTTTACTATACTGAGCCAGGATACATTTAGTTTA +AATTCATGGTGTGCTCCATTTATTTTGAAGGAGTATACATTACAATACATTTAGATATGGGAATTAAAAA +TTTTGTAACTGCATCTAGGATCCATATATCTTTATTGTTTTGAGCCGCGGTATGATGTCATCCGTCAAGC +GGGTTACCCAGGACAGAATTCATCAAACAGGACTTCTAACCGCATACTAAACTGGATAATTTCCGCTTTT +GGTCATTGTTTTTTTGTATATCACATGTGGAATTTTCCGCCCATTCGGCAGGAACAAATGACCACAATGA +GCCGAATTCTATTATTTACAAGCAGTTATCCTAGTTTCTGAATTGCAATTAGCATACATTCGTTTGTGCA +GAATAAATATCCTACTTATTCTTCGTTCGTTCAGCTCAATAGTTCTACGACGAAAACTCACAAGCTTACT +TATTGCAGCCTGCAGCACGCCCCACCTGTCAACAATTTTTTTCTCTGGGGTTATACAGCACCCTTGAAAA +CTTTTCACTAGTACATATTACACACTCTTTTTCCTGCTAAGCGACCAAATGTTGAAGTCAACAGGACAGT +TACTGAAGGATGTTTTCATACCTCTTCCAGCAAGTAAACGTGCACACACGCAATTGCACACAACCCATGG +TGAACATCACCATCATGTTAGTGATGACTACAAGTTCCCTATAAACGACACACAATCGATCAGTGATGCC +TCTACTTTGGTTCCACAGACCCACAGAAAACTAAAGAACAGACATGTGCAACTTATTGGTATCTCTGGTG +TCATTGGTACTGCTCTTTTTGTTGCAATTGGTGGTGCACTACAAAAGGCTGGTCCTGGGTTCTTATTAAT +TGGATTCACCGTTTGGTGTATTCCAATTTTATGTATCACTGTCTCTACAGCAGAAATGGTGTGCTATCTA +CCGATAAACTCCCCCTTTATAAGAATGGCTGGCCGTTGCTGTGACGATGCTCTTGAATTTATGGCTGGCT +GGAATTTCTGGTTCCTGGAATGTGTCTTGATTCCATTTGAAGTTGTCGCCGTGAACACAATTATTCATTA +CTGGAGGGACGATTATTCCCCTGTGATTACCCTGATAGTGCAGATTGTTATTTATTTCTTGATTTCAATT +TTTGCTGTTAGAATTTATGGCGAGACTGAGTTTTGGTTGGCTATTGGTAAAGTTTTGCTGGCTGTTGGTT +TAATTTTGTTTACTTTTATCACCATGGTTGGTGGTAACCCACAGCATCATGCATTTGGTTTCCAATTCTG +GAAGAATCCGGGTGCATTTAAGCCATACTGGTCGACTGGTTCATTAGGATATTTCCAAGCCTTCATTACA +TGCCTCACACAGGCATCTTTCACCATAGCAGGACCAGACTATCTCTCCATGGTTGCTGGTGAAACTGTGC +TTCCAAAGTCATCGACTCTTCCAAGAGCATTCAAGCAGATATTCTATAGATTGACCTTCTTGTTTATCGG +AGGTGCTCTATGTGTTGGTATTTTAGTTGCCAGTGATGACGCTGATTTAACTGCAGCCATTAATCAGTCA +AGACCAGGTGCTGCTTCCAGTCCTTACGTCATTGCAATGAACAATATGGGAATTAAAGTATTGCCAGATA +TTGTTAATGGTGCGTTGGTTGTTGCAGCTTTTAGCGCAGGGAATGCCTATACTTATTGTTCATCGAGAAC +ACTGTATGGTATGGCTCTCGAAGGAAAAGCTCCATTTATCTTCACATATTGTAACAAGCAGGGTGTCCCA +ATCCTAGCTGTCCTTGTTTCATTGGCATGGGCACTATTGTCATTCTTGCAATTGAATAACAACTCCGCTG +TTGTCTTAAATTGGTTAATAAATCTGATCACTGCGTCGCAACTGATCAATTTTTGTTTGATGATGGTCAC +TTACTACTTTTTCTACAAAGCGGTATTGGCGCAAGGTATTAACAGAGATGAATTCACCTTCAAGAGTTGG +TGGCAACCATATACTAGTATTTTTGGCTTCACTTGGCCTTTCATTATGATGTGGGTCAACGGTTACACTG +TCTTCATCAAAGGACACTGGGAAGTTTCAGCCTTCCTGTTCAACTATCTGGCCATCTTTGTCTGTATTGG +TCTATATAGTTTCTGGAAGGTCTTGAAGAGATGTAAGTGGAAAAACCCTCTGGAAGTAGATCTGGTGTCT +GGGTTGCAAGAGATTGAAGAACATGAGGCTCAATACTACCAAGAACTGGAGGAAATGAAGAGGCTCAAAG +GTGAGAAGAAAGGAATTGCCAAATTCTTTGACAGTGTCCTATCCCTACTTGTCGGAAAGGATTGATTATT +TATTATAGAGCTACCTAGAATATATTTTAATTGTTGATATAGTTCTTTGGCTGTATAGGGTTTAATGGTG +AAATCTCGATCGTGTCTCTATCAAAAAGTGCACAGCAAAGTGCGGAGATGAAGATCCCTGTTCGACGGAA +CAATAATTGTATACTATTTGACACAGCCCGATCACGCTATTTATAACTTGGGTACAGCCATTCTATCAAG +TATTGTTTTGAAAAGTGGTCAATAATCAGATGTCTTACGATTAGTTATGTTAGTAATTTGAACTAGATTG +GGGGATAGTAAGCTAAAAAATAATGCAACTGACACCATCTACAAGGGGGTCATCTAGAACGGGGATTTCA +TTTCTAGAATTACTTCATGAGCCACAACCAAGCCTACTTACTTGCCCATTCTGGGTTAAGGTTTTAGTAT +AATCCTGGTATGAATTGCTATGAACTACACTCAGAACAGATTCCAGAGCTGTGCAATTAAGATATCTTGA +GGGAGAGGTGTCACATTTCCAAAGATATAAAAGGCCCAAGAGGTCTCTGCATAACCTTTAATATATTTCT +TTCGAACAGTACAAGAGCGTATTCATTCACTAATAATGCAACTCACTTCTTTAATCTCCTTGTTGTCCCT +AGCTGTCCTTGGCGTTTCCCAAGCTACGGACGCCCAAAGTGAAGGTGCAGTTCCAGCTACTACTACTGCG +GCTGATGCTGCCACTACTACCGATGCTGCTGCTGCTACTGATGCTGCTGCTACTGATGCTGCGGGAGAAG +ACGATGCTGCCGACGAAGACGATGGTACGGGAGAGGACGATGAGGATTATACTGATGATTACGATGAGGA +CTATACTGATGATTACGATGAGGATTATACCGATGATGCAGAGGATACAGCCACTGAAGCTTCTGAATCG +TCGGCTGCAGAAACCCCTGTGGTTTCACAAATTTCTGATGGCCAGGTTCAGGCCACTGCTACCCCAACTG +TTGCTGTTCAATCTGATAACGGTGTTGGCAAATTCGCTCCAAGTGCTGCAGTTGCCATTGGTATCGCTGC +CTTCTTGATATAATCTTATTGATCATGAATGATGCTATTCAAATCGTTTTATTTATCTCTCTTTTATTCT +GCATTATGTAGTGACAGGTATCGTAAGGATCATCACGTCATGACACATGAACTTCCAACGCACTACAAAT +GGTTCAAAAAAAAACACACAGGATCTATGTATATTATGGAGGACTAATCCTTCTTTGAGTCCTTTGGCTC +TCCTGCTTTCTCGCTGCTGGCTTCTGTAGACTCTTTAGATTCATGCACTTCATCATCGTCCAAGTTTTTC +TTATCTTGAACTTGAATGATAATACCATCATCAGTATCTTGCTTGGTTTCAGACTCAATCAGCTCTTGCT +GTTCCTTCAGCAGTTCTAATCTTTGTTTGTTACTAACCTCTTCGACAACATCAGCCTCAGCAACATGGTA +CACTTCTTCAGGCATCGAACTTAGCACCGCTTGAAGTGCATCATAAATGGTTTCATCAGAGTCCAAATCA +CCGTAAGTGTATGCGTTGGACAACACAAGCAGGGTGGATGGGATTCTCTCATTTAATCTCAAGTTTAACC +AAGCAGTAAGATCGCGTTTCAATTCGCTCACTGGGACACCACTGACCCTGATACCTCTGGAGCTGCATGC +AGCTTGCAGCTCAACTGTGCTCAAGCTATCAATACCCTCATAGTAAATGGCTTTGTCATCTCTCTTGATA +TTCAGCAGTTTGTTTCTGATTCTGAATGTAAGCATTTGATTGGTGCCGATTGGTTGAAGATTAATGTATT +TTGCCACAGCAACCAGCTGAGATCTTTGTAGGTTATCCAAGACAGTGTCATCTTTGAACAGTTTGGCTAC +TCTAAGTAGCTGTTCTCTAGATGGCTGCTCTGAAGACGATCTAATCTTGACCAAGAAGTCTTTGAAATCC +GCTTTTTGTTCCTCAGTAAGAGCACTTGGAAACTTCATCTGGATAGAATCCTTCAGCAGTTGAGACACAC +TGGTTCTGGTCTTTAGGAGCTTTGAACGCTTTTTTTCCTTGTCTGCCGAAGATTCATATGTTGATGGCAA +CAGGTTTGGGAACATCTTCAAGGCGACTGGCAACAGAAGTTCAGCGAATGGGACAATCACAAACATCGAA +AATGGAACAAGTCTAACGATATCTTGTGTAGTTCTTTGTAACTGTTTATTCTCCCTTCTGGTCAACTCGT +AACCAGCAGCAGACTTCAACGCCAACTTGAAAGAGACTTTGATCTCAAATCCCAAAAGTTTAGTTCCTTC +CCAGTAATGGTTCACTTCATGTTTCACCTTTTCCCAGATGGTCTTCTTAGCAGGCTCTGTGAC + diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.lift.gff b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.lift.gff new file mode 100644 index 00000000..35d9c2ff --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.lift.gff @@ -0,0 +1,22 @@ +##gff-version 3 +##PAF BC_dic1 316 0 316 - QBLI01000035.1:1-51373 51373 711 1659 948 948 0 AS:i:1604 ms:i:1604 np:i:316 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:316M cs:Z::316 +QBLI01000035.1:1-51373 miniprot mRNA 709 1659 1604 - . ID=L000001;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_dic1 1 316 +QBLI01000035.1:1-51373 miniprot CDS 709 1659 1604 - 0 Parent=L000001;Rank=1;Identity=1.0000;Target=BC_dic1 1 316 +QBLI01000035.1:1-51373 miniprot stop_codon 709 711 0 - 0 Parent=L000001;Rank=1 +##PAF BC_MATa2 269 0 269 - QBLI01000035.1:1-51373 51373 9115 9978 804 807 0 AS:i:1361 ms:i:1390 np:i:268 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:96M56N173M cs:Z::50*aatT:45~gt56ag:173 +QBLI01000035.1:1-51373 miniprot mRNA 9113 9978 1390 - . ID=L000002;Rank=1;Identity=0.9963;Positive=0.9963;Target=BC_MATa2 1 269 +QBLI01000035.1:1-51373 miniprot CDS 9691 9978 472 - 0 Parent=L000002;Rank=1;Identity=0.9896;Target=BC_MATa2 1 96 +QBLI01000035.1:1-51373 miniprot CDS 9113 9634 918 - 0 Parent=L000002;Rank=1;Identity=1.0000;Target=BC_MATa2 97 269 +QBLI01000035.1:1-51373 miniprot stop_codon 9113 9115 0 - 0 Parent=L000002;Rank=1 +##PAF BC_MATa1 162 0 162 + QBLI01000035.1:1-51373 51373 10602 11088 486 486 0 AS:i:812 ms:i:812 np:i:162 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:162M cs:Z::162 +QBLI01000035.1:1-51373 miniprot mRNA 10603 11091 812 + . ID=L000003;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATa1 1 162 +QBLI01000035.1:1-51373 miniprot CDS 10603 11091 812 + 0 Parent=L000003;Rank=1;Identity=1.0000;Target=BC_MATa1 1 162 +QBLI01000035.1:1-51373 miniprot stop_codon 11089 11091 0 + 0 Parent=L000003;Rank=1 +##PAF BC_MATalpha1 233 0 233 + QBLI01000035.1:1-51373 51373 13925 14624 699 699 0 AS:i:1232 ms:i:1232 np:i:233 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:233M cs:Z::233 +QBLI01000035.1:1-51373 miniprot mRNA 13926 14627 1232 + . ID=L000004;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATalpha1 1 233 +QBLI01000035.1:1-51373 miniprot CDS 13926 14627 1232 + 0 Parent=L000004;Rank=1;Identity=1.0000;Target=BC_MATalpha1 1 233 +QBLI01000035.1:1-51373 miniprot stop_codon 14625 14627 0 + 0 Parent=L000004;Rank=1 +##PAF BC_sla2 1046 0 1046 - QBLI01000035.1:1-51373 51373 23253 26391 3138 3138 0 AS:i:5179 ms:i:5179 np:i:1046 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:1046M cs:Z::1046 +QBLI01000035.1:1-51373 miniprot mRNA 23251 26391 5179 - . ID=L000005;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_sla2 1 1046 +QBLI01000035.1:1-51373 miniprot CDS 23251 26391 5179 - 0 Parent=L000005;Rank=1;Identity=1.0000;Target=BC_sla2 1 1046 +QBLI01000035.1:1-51373 miniprot stop_codon 23251 23253 0 - 0 Parent=L000005;Rank=1 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.miniprot.gff b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.miniprot.gff new file mode 100644 index 00000000..593d6b60 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_QBLI01000035.1_1-51373.miniprot.gff @@ -0,0 +1,84 @@ +##gff-version 3 +##PAF MH260578_SLA2 1072 29 1072 - QBLI01000035.1:1-51373 51373 23256 26370 1719 3165 0 AS:i:2775 ms:i:2775 np:i:763 fs:i:0 st:i:0 da:i:21 do:i:3 cg:Z:94M2D73M1D99M1D18M7I14M2I256M5I54M4D136M1I29M1I11M1I183M4D59M cs:Z:*tctT:1*ctcI*cagS:2*cttI*cgtK:2*acgC*tctT*gctP*gatE*gagD:2*ccaS:18*ctcK*tcgD:2*aacE*gctT*ttaM:1*attV*ttgV:1*attL*cagK:1*gatT*gaaD*gttT:7*accL:9*caaP:3*gcaV*gaaG:1*tacF*aggK:1*ttgM*aacE:2*gatS*ggtN:1*gggD*agaT*tctL*ataR*caaT*ggtL:1-ggattc*aagR*ggcR*tatF*ggtQ*agaV:2*aaaP:3*accN:1*ttgI*atcL*agaQ:2*cagR:2*cggH:14*atcV:2*gtgR:2*caaS*aacD:1*gatN:3*gaaD:2*atgL:2*atgT:7*gatS*ctgF*cagG:1*cttD:4*attV-tca*catR*gatN*aagN*agaN:3*aaaI:6*cttI:1*actA:8*atcL*accI:5*attM:1*cgtK*tccM*acaS*ggaD:1*gatA*gatE*gctI:5*cagS*cgcK*tacF:1*tcgD:2*gctE:2*ttcY*gaaD:3*gatN:2*tccN:5*tctT:5*agaK:2*gagF*acaE:4*atgI*gttI*aagQ*gatN*gaaD*tcaE*tccD*gtcE*gaaN*gagV*gagN*actL:1-aga:1*gaaL*tctT*cgtE:1*acaK*aatT*ttgA*tcaA*gaaT*ccaT*attL*tctR*gagH*cagS:2*gtcP+FEQENER*tccQ:1*ttcQ:1*caaA:3*gcaT:4*gtcM+VR*gatN*tttA*tggF*agcV*aatE:2*tctQ*agtL*tttY:1*gctQ*gagQ:1*attQ*cgtQ:1*caaE:1*gagQ:1*gatQ:1*caaE*gctL*ctcQ:4*tatQ*gctQ*gaaL*caaA:1*agaQ*attQ*ttgY*ttcW:4*gtcR:2*caaA:1*cagA:1*cgtQ*attL:2*gagQ:3*cgcL*gagD:1*tacA:1*tcaR*caaH:11*ctgI:1*gctT:1*agaK:3*gagD*caaN:3*ctaM:2*caaS:3*cgtK:1*aagQ:3*actN:1*attL*acaA*gctS:3*tcaT:1*actQ*cagE:1*attL*ggcA:4*ttgQ*attL*caaN*aacL:1*catQ:2*ataV*actE*ctaY:16*cagT:6*aatT:1*ttcS:2*ctcI:11*gacE:6*aagR:1*ctgM:1*tcgA:2*gtgI:3*ggtD:2*cgtK:7*gacE:2*aaaR*gttF:1*ggtN:1*aaaT*ggtK*gatT*gagD*attL*gacQ*aagT:1*gagQ:1*caaE*attK*agaD*gatE:1*accE*ttgQ:2*gatA*gatT*gccL*gagD:1*aacA:2*tcgA:2*tccT*actA:2*gctN:2*aagN:2*atcL*gaaQ*gctE:1*gagQ:1*cagK+FKDVT:1*agaN*cagA*caaP:1*tcgD*gctE*ttcK*aatL*ccaK*gaaQ:2*caaE*ttaQ:1*aagQ*gatE:2*gaaL:1*ttaI:1*attM*ctcM:5*gatE*gcgT*ctgI*gtgK*gaaD:1*gccV:1*gctT:1*aagN*gagS*aatR:1*acaE*gctK*attS:1*gagM:1*atcN*gacK*caaL-gttttggttgat*cacN*ttaS:2*cttV*actL*tccG:1*gttI:4*ctcK:2*ctcI*aagN:1*ttaI:3*ttaV*tatF:10*cagL:6*ttaV:1*tccT*attL*atcL:2*gcgS:1*tccG*tctL*gctV*atgT:2*ggtA*gacS:3*gacN*ttcM*atcL*attV:2*ccaD:1*ggaA*gatN*cacE:1*gagL*attV:1*cgtD:1*ctcI*aatT*gacN:2*tctT:2*gctS:1*gttI:3*tctT:2*attL*gtgT:2*gttT:1*tcaQ:1*gcaD*gagF*gctQ:1*caaD*ttcL:1*gctD:2*agaK*gctD*actI:2*gaaI:2*gaaV*ttcY*ttcL*aaaE:2*cttY*tccH*gatS*agtN:1*gagV*ggcN*gaaK+S*gtgI*gaaD*gagD*aagQ:2*gtgI:1*atgI*gccN:2*ttgI*gcaD:3*aaaI:2*gatT:1*accL:2*actV:1*gacS*ttaM+K*gccT:1*agaS:1*aatK*cttI*ttgD*aacL:1*aacK:1+D*gcgG*aacE:1*ggcS:3*gaaD*actK:1*ttgM*cagE*aatH:1*gccS*gccR*tccV:1*tcaE*aagE:2*tccV*cagH:1*actS:3*tcgN*aaaQ*gatP*caaF:1*ccaV:1*atcM:1*caaS:2*tttI:1*attV:2*gctS:1*gttL*tccA:2*attS:1*gttI*accI*aatT*tccA:1*gaaK*cttY:2*aaaR:1*gctS:1*gctE:6*gcaN*aagN*aacG*aaaR:2*attH:3*gaaS*tacF:3*cacN:1*cgtK:8*gctS:2*gttI:1*cttY:2*aagN*attT:2*gaaR*actI:5*tctQ*gggN*aatK*aaaH:1*catN:1*cagE:1*attV:3*catR:6*gttA:3*gctS:1*tcgA:3*gctS*acgQ:1*ttgM:4*gacN*aatK:2*actD:3*aacK:1*acaN*tctL:2*agaK*agtQ:2*aatD:2*caaN*gacK*attL*ttaI*actI-cagagaagcaac:1*ggtK*agtH*ggtE*gagV*aatE:1*gttI:5*acaS*aatI:5*gtgA:15*atcS*gcaS:10*tatF*ggcS:1*agaK:1*gggD:2*agcD:2 +QBLI01000035.1:1-51373 miniprot mRNA 23257 26370 2775 - . ID=BC000001;Rank=1;Identity=0.5431;Positive=0.7232;Target=MH260578_SLA2 30 1072 +QBLI01000035.1:1-51373 miniprot CDS 23257 26370 2775 - 0 Parent=BC000001;Rank=1;Identity=0.5431;Target=MH260578_SLA2 30 1072 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 195 44 154 + QBLI01000035.1:1-51373 51373 14099 14435 120 339 0 AS:i:161 ms:i:161 np:i:58 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaT*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaK:2*agtL*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS*agaS*aagR*agaH*gggR*cccV*tcaQ*agaK*aagE*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatR:2 +QBLI01000035.1:1-51373 miniprot mRNA 14100 14435 161 + . ID=BC000002;Rank=1;Identity=0.3540;Positive=0.5133;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 45 154 +QBLI01000035.1:1-51373 miniprot CDS 14100 14435 161 + 0 Parent=BC000002;Rank=1;Identity=0.3540;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 45 154 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATa1 181 122 181 + QBLI01000035.1:1-51373 51373 10911 11088 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +QBLI01000035.1:1-51373 miniprot mRNA 10912 11091 171 + . ID=BC000003;Rank=1;Identity=0.5593;Positive=0.7458;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +QBLI01000035.1:1-51373 miniprot CDS 10912 11091 171 + 0 Parent=BC000003;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +QBLI01000035.1:1-51373 miniprot stop_codon 11089 11091 0 + 0 Parent=BC000003;Rank=1 +##PAF 5476_sc5314_MTL_A_MTLA1 210 116 197 + QBLI01000035.1:1-51373 51373 10860 11079 105 243 0 AS:i:169 ms:i:158 np:i:49 fs:i:0 st:i:0 da:i:24 do:i:9 cg:Z:14M7I1M1I58M cs:Z::2*gaaD*gcaQ*gttM*atgD*gccD*tcaD*ccaK:2*aatE*aacD*aatD+ILIKGEE:1+K*caaK*caaS:2*cgaK:1*agaQ*catR*attL*tccD*aagN*gaaS*gctT*cgcK*agcE*cttF:3*gcaV:2*actK*actN:1*aatQ:1*accN:1*aagR:2*gcaE*ataL:4*tccH:1*atgV*acaS*gcaL*cttS:1*gtaI:10*accK:1 +QBLI01000035.1:1-51373 miniprot mRNA 10861 11079 158 + . ID=BC000004;Rank=1;Identity=0.4321;Positive=0.6049;Target=5476_sc5314_MTL_A_MTLA1 117 197 +QBLI01000035.1:1-51373 miniprot CDS 10861 11079 158 + 0 Parent=BC000004;Rank=1;Identity=0.4321;Target=5476_sc5314_MTL_A_MTLA1 117 197 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1019 7 1019 - QBLI01000035.1:1-51373 51373 23265 26376 1533 3135 0 AS:i:2456 ms:i:2448 np:i:714 fs:i:0 st:i:0 da:i:15 do:i:12 cg:Z:259M1I6M3I15M7D7M4D5M2I254M6D35M4D11M7D70M1I34M1D25M1I227M4D56M cs:Z::1*gagN*tctL:1*ctcI:1*actA*gccS:1*cgtK:2*acgC*tctT:2*gagA*actA:20*ctcR*tcgE:2*aacH*gctC:2*attL:4*aatS*gatN*gaaD*gttT:1*ttaI:2*gctT:2*accV:9*caaP*tctT*gctC:1*gcaI*gaaG:2*aggK:1*ttgI*aacS:1*atcL*gatE*ggtS:1*gggS:1*tctF*ataS*caaN*ggtN*gatG*ggaT*ttcA*aagA:2*ggtT:7*accF:2*atcE*agaQ:2*cagK:2*cggH*aatD:6*accM:6*atcV:2*gtgR:2*caaS*aacD:1*gatN:2*tatF:1*gccS:4*atgL:7*gatN:3*cttV:2*gcaS*acaS:1*tcaR:1*gatT*aagS*agaE:3*aaaV:2*gcaS:3*cttI*gttI*actA:8*atcL*accI:3*aggK:1*attL:1*cgtK:1*acaS*ggaE:2*gatE*gctV*ttaI*gagA:3*cagD:1*tacF*gagD*tcgV:2*gctR:12*cgcK:3*tctT:1*atcV:6*gagS*acaS*gctP:1*aacI:1*atgQ+D*gttE*aagE:1*gaaP*tcaQ*tccG+FPL:1*gaaR:1*gagR*actS*ccaS*agaV*ccaS*gaaN:1*cgtE*cagE*acaS:1*ttgI-tcagaaccaatttctgagcag:1*actR*gtcS:1*cagN*ttcI:1-caaccaattgct*gcaS:4+AM:2*tttS*tggF*agcV*aatR:2*tctQ*agtE*tttL:1*gctE*gagQ:1*attR*cgtQ*ttgA*caaE*caaE*gagR:1*gatQ:1*caaE*gctL:1*caaE:2*cagM*tatL*gctQ*gaaR*caaD:1*agaA*attR*ttgF*ttcW:1*cagE:2*gtcR:2*caaA*gaaV*cagS:1*cgtE*attL:2*gagS:3*cgcA*gagD:1*tacM:1*tcaR:4*cgcK*gtaL:1*gagQ:2*cgcQ:3*gctT:1*agaG:2*tatH:1*caaR:3*ctaM:1*cagE:7*gccV:2*actN:1*attL*acaR*gctI*accV:2*tcaT:1*actE*cagE:1*attL*ggcA*tccV:1*gatT*gagS*ttgQ*attC*caaS*aacH:1*catE:2*ataV*actQ*ctaY:23*aatG:3*aaaI*ctcV:9*gccS:12*tcgA:2*gtgI:3*ggtD:2*cgtK:9*gagA*aaaR*gttI:2*ggtN*aaaD*ggtS*gatE*gagQ*attV*gacQ*aagM:1*gagR*ttgT:1*attL:2*ctcM*accE*ttgT:2*gatK*gatS*gccL:1*agaE*aacT*caaH:1*tcgQ:2*tccA*actS*attL:1*gctS:2*aagS:4*gctE*ttgI-gagaaccagttaagacag*caaK*tctA:2*ttcS*aatE*ccaL*gaaK*ttgE*gaaG*caaG:1*ctaN*aagE*gatE*aagA*gatQ*gaaQ:1*ttaV*gaaQ*attM:3*acaA:3*gcgT*ctgM*gtgQ:3-aaagctcagaag:1*aatQ*gaaH*acaQ:3*gagK*cagE*atcA*gacE-caagttttggttgatcactta:1*aagR*cttV*actL*tccR:1*gttI:1*gctT*atcL:1*ctcK*agtV*ggcS*ctcS*aagD:2*caaR*gacE*gccF*ttaV*tatF*gaaN:1*gacE:2*atgV*caaY*gctS*ggtN*aacT*cagD*aacI*tcaP*tcgT*cctA:3*ctcG*tccT*attM*atcL:1*aaaR*gcgV:1*tccH*tctL*gctS*atgT*gacQ:1*ggtS*gacT*tctT*tttM*aatT:1*ttcY:1*attA:1*gggN*ccaM*aatK:1*gatE+S*cacE*gctS*gagG*attL:1*cgtK*tctA*ctcI*aatI*gacE*ttcL:1*tctD:2*gctG*gacN*gttL*ttaF*ttaS:1*tctV:1*ggtA*attV*gtgL:1*cttD*gttR:1*tcaD:1*gcaV*gagQ-gct*gatE*caaI*ttcL*ataV*gctE*actG*gcgS*agaK*gctD*actL:1*gagH*gaaM*tcgV:1*gaaL:2*aaaE:1*ttgM:2*gatN*agtH+L:1*gagK*ggcL:1*gtgI*gaaH:1*aagR:2*gtgR:1*atgI*gccT:2*ttgI*gcaN:3*aaaV*cttI:1*gatS:1*accM:1*ttgV*actV:1*gacV*ttaF*gccR*ccaD*agaP*tctG*aatR*cttK*ttgT*aacP*agcV*aacS*gttS*gcgD*aacE:2*gacY*cttF*gtgL*gaaD*actK:2*cagA:1*gcgT*gccQ:1*tccA*attV*tcaE:2*tcaA*tccG*cagL:1*actE:1*ctaM*ctcS*tcgT*aaaR*gatH*caaK*gacL*ccaE*tctV*atcQ*tcaE*caaD*atcG*gacS*tttL:1*attV:1*aaaE:2*gttI:1*gctC:1*attS:1*gttI*accI:1*tccA*attV*gaaK:2*attL*aaaE:1*gctC:1*gctD*tcaA:5*gcaS*aagR*aacG*aaaR:2*attM:1*aagR*actA*gaaL*tacF:3*cacN:10*gctS:1*tctQ:2*cttY*tccA:1*aagG:3*gaaR*actM:5*tctA:1*aatT*aaaN:1*catS:1*cagE:5*catN:3*gctS*tcaA:1*gttA:3*gctS*gctS:4*gctS*acgD*tttL*ttgM:1*aagQ*actS*caaH*gacL:3*actE:2*aagR*aacK:3*gccS:1*agaK*agtM:2*aatT*caaK:1*caaR*gacS*attL:1*actL-cagagaagcaac*ggcS*ggtD*agtE*ggtS*gagN*aatK*gatT:4*aaaQ:2*aatA:3*aaaR:1*gtgA:1*atgL:10*aatS:2*atcS*gcaH:10*tatF*ggcS:3*gggE*gacE:1 +QBLI01000035.1:1-51373 miniprot mRNA 23266 26376 2448 - . ID=BC000005;Rank=1;Identity=0.4890;Positive=0.6833;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +QBLI01000035.1:1-51373 miniprot CDS 23266 26376 2448 - 0 Parent=BC000005;Rank=1;Identity=0.4890;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 187 128 187 + QBLI01000035.1:1-51373 51373 10911 11088 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +QBLI01000035.1:1-51373 miniprot mRNA 10912 11091 171 + . ID=BC000006;Rank=1;Identity=0.5593;Positive=0.7458;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +QBLI01000035.1:1-51373 miniprot CDS 10912 11091 171 + 0 Parent=BC000006;Rank=1;Identity=0.5593;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +QBLI01000035.1:1-51373 miniprot stop_codon 11089 11091 0 + 0 Parent=BC000006;Rank=1 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 190 41 151 + QBLI01000035.1:1-51373 51373 14099 14435 126 339 0 AS:i:172 ms:i:172 np:i:59 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaS*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaN:3*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS:1*aagR*agaH*gggR*cccV*tcaQ*agaK*aagQ*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatI:2 +QBLI01000035.1:1-51373 miniprot mRNA 14100 14435 172 + . ID=BC000007;Rank=1;Identity=0.3717;Positive=0.5221;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 42 151 +QBLI01000035.1:1-51373 miniprot CDS 14100 14435 172 + 0 Parent=BC000007;Rank=1;Identity=0.3717;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 42 151 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 294 0 294 - QBLI01000035.1:1-51373 51373 711 1587 504 882 0 AS:i:856 ms:i:856 np:i:214 fs:i:0 st:i:0 da:i:72 do:i:0 cg:Z:98M1I99M1I95M cs:Z:*gccM:1*gctS*aaaD*accK*ataS:1*gccK*accS*aagI*accA:1*accS*accA*aagN*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctK*ttaV:1*aagG*ccaD:2*tttV*cagS*atgL:2*tcgK:1*ctgI:1*gcaT*gatE:1*attV*acaL*ggtA*atcA:1*tctA:2*tctT:1*ggcS:8*ctgT*accA:3*gccV:2*tacK:3*agtI*ttcM*gttT+D:1*gccT*gatR*aagG*atgQ*agtA:1*atgT*gctF*tatQ:2*cccA*atcA:2*gttI*agtA:5*atcV:4*tctA:1*attV:8*actN*ggtS:2*atcE*gaaS:3*aacH:2*aatH:1*tttL:2*gtgL*gccL*cgaK:1*gtgT*actR:2*ggtN*cctL*agtT:6*tctG*acaP:2*gtgA:2*gttI:3*tcaA:4*actS:2*ttgV:2*gaaK*atgL:1*atcV+E*tccN*ttcL*ggaS:1*aaaD*aagP*gagK*tccT*ttcK*ttcA:1*tttH:2*tctA:2*cttI:3*gccV:3*attV:13*gcaS:3*aacG*catQ:1*gtgS*gtgF*cacG:2*actK*aaaD:1*ttgI*aagS*gatR:2*cccL*agcG:6*gtgT:1*agtA:1*gtgI:4*aatH:2*atcL*attT:1*ctcI*gttA*gtaL:3*aagR:1*atgL*agaK:3 +QBLI01000035.1:1-51373 miniprot mRNA 709 1587 856 - . ID=BC000008;Rank=1;Identity=0.5714;Positive=0.7279;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +QBLI01000035.1:1-51373 miniprot CDS 709 1587 856 - 0 Parent=BC000008;Rank=1;Identity=0.5714;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +QBLI01000035.1:1-51373 miniprot stop_codon 709 711 0 - 0 Parent=BC000008;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 305 7 305 - QBLI01000035.1:1-51373 51373 711 1590 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gatK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +QBLI01000035.1:1-51373 miniprot mRNA 709 1590 818 - . ID=BC000009;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 8 305 +QBLI01000035.1:1-51373 miniprot CDS 709 1590 818 - 0 Parent=BC000009;Rank=1;Identity=0.5537;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 8 305 +QBLI01000035.1:1-51373 miniprot stop_codon 709 711 0 - 0 Parent=BC000009;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1045 0 1045 - QBLI01000035.1:1-51373 51373 23265 26385 1827 3165 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D54M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gagS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE*agtE +QBLI01000035.1:1-51373 miniprot mRNA 23266 26385 2946 - . ID=BC000010;Rank=1;Identity=0.5773;Positive=0.7488;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +QBLI01000035.1:1-51373 miniprot CDS 23266 26385 2946 - 0 Parent=BC000010;Rank=1;Identity=0.5773;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1044 0 1044 - QBLI01000035.1:1-51373 51373 23268 26385 1827 3162 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:15 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D53M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gagS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE +QBLI01000035.1:1-51373 miniprot mRNA 23269 26385 2946 - . ID=BC000011;Rank=1;Identity=0.5778;Positive=0.7495;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +QBLI01000035.1:1-51373 miniprot CDS 23269 26385 2946 - 0 Parent=BC000011;Rank=1;Identity=0.5778;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 305 7 305 - QBLI01000035.1:1-51373 51373 711 1590 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gatK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +QBLI01000035.1:1-51373 miniprot mRNA 709 1590 818 - . ID=BC000012;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 8 305 +QBLI01000035.1:1-51373 miniprot CDS 709 1590 818 - 0 Parent=BC000012;Rank=1;Identity=0.5537;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 8 305 +QBLI01000035.1:1-51373 miniprot stop_codon 709 711 0 - 0 Parent=BC000012;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 305 7 305 - QBLI01000035.1:1-51373 51373 711 1590 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gatK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +QBLI01000035.1:1-51373 miniprot mRNA 709 1590 818 - . ID=BC000013;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 8 305 +QBLI01000035.1:1-51373 miniprot CDS 709 1590 818 - 0 Parent=BC000013;Rank=1;Identity=0.5537;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 8 305 +QBLI01000035.1:1-51373 miniprot stop_codon 709 711 0 - 0 Parent=BC000013;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1044 0 1044 - QBLI01000035.1:1-51373 51373 23268 26385 1827 3162 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:15 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D53M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gagS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE +QBLI01000035.1:1-51373 miniprot mRNA 23269 26385 2946 - . ID=BC000014;Rank=1;Identity=0.5778;Positive=0.7495;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +QBLI01000035.1:1-51373 miniprot CDS 23269 26385 2946 - 0 Parent=BC000014;Rank=1;Identity=0.5778;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 287 14 287 - QBLI01000035.1:1-51373 51373 711 1524 477 819 0 AS:i:799 ms:i:799 np:i:201 fs:i:0 st:i:0 da:i:135 do:i:0 cg:Z:77M1I99M1I95M cs:Z::1*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctK*ttaV:1*aagG*ccaD:2*tttV*cagS*atgL:1*ttcY*tcgK:1*ctgV:1*gcaT*gatE:1*attV*acaL*ggtA*atcA:1*tctA:4*ggcS:8*ctgT*accA:3*gccV:2*tacK:2*gagG*agtI*ttcM*gttT+D:1*gccT*gatK*aagG*atgQ*agtA:1*atgT*gctF*tatQ:2*cccA*atcA:2*gttI*agtA:5*atcV:4*tctA:1*attV:8*actN*ggtS:2*atcE*gaaS:3*aacH:2*aatH:1*tttL:2*gtgL*gccL*cgaK:1*gtgT*actR:2*ggtN*cctI*agtT:6*tctG*acaP:2*gtgA:2*gttI:3*tcaA:4*actS:2*ttgV:2*gaaK*atgL:1*atcV+E*tccN*ttcL*ggaS:1*aaaD*aagP*gagK*tccT*ttcK*ttcA:1*tttH:2*tctA:2*cttI:3*gccV:3*attV:13*gcaS:3*aacG*catQ:1*gtgS*gtgF*cacG:2*actK*aaaD:1*ttgI*aagS*gatR:2*cccL*agcG:6*gtgT:1*agtA:1*gtgI:4*aatH:2*atcL*attT:1*ctcI*gttA*gtaL:3*aagR:1*atgL*agaK:3 +QBLI01000035.1:1-51373 miniprot mRNA 709 1524 799 - . ID=BC000015;Rank=1;Identity=0.5824;Positive=0.7363;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +QBLI01000035.1:1-51373 miniprot CDS 709 1524 799 - 0 Parent=BC000015;Rank=1;Identity=0.5824;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +QBLI01000035.1:1-51373 miniprot stop_codon 709 711 0 - 0 Parent=BC000015;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 195 44 154 + QBLI01000035.1:1-51373 51373 14099 14435 120 339 0 AS:i:161 ms:i:161 np:i:58 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaT*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaK:2*agtL*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS*agaS*aagR*agaH*gggR*cccV*tcaQ*agaK*aagE*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatR:2 +QBLI01000035.1:1-51373 miniprot mRNA 14100 14435 161 + . ID=BC000016;Rank=1;Identity=0.3540;Positive=0.5133;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 45 154 +QBLI01000035.1:1-51373 miniprot CDS 14100 14435 161 + 0 Parent=BC000016;Rank=1;Identity=0.3540;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 45 154 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 181 122 181 + QBLI01000035.1:1-51373 51373 10911 11088 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +QBLI01000035.1:1-51373 miniprot mRNA 10912 11091 171 + . ID=BC000017;Rank=1;Identity=0.5593;Positive=0.7458;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +QBLI01000035.1:1-51373 miniprot CDS 10912 11091 171 + 0 Parent=BC000017;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +QBLI01000035.1:1-51373 miniprot stop_codon 11089 11091 0 + 0 Parent=BC000017;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 440 0 440 - QBLI01000035.1:1-51373 51373 23265 24627 591 1371 0 AS:i:863 ms:i:863 np:i:295 fs:i:0 st:i:0 da:i:975 do:i:12 cg:Z:15M4D11M7D70M1I34M1D25M1I224M1I7M5D51M cs:Z:*gaaQ*attM:3*acaA:3*gcgT*ctgM*gtgQ:3-aaagctcagaag:1*aatQ*gaaH*acaQ:3*gagK*cagE*atcA*gacE-caagttttggttgatcactta:1*aagR*cttV*actL*tccR:1*gttI:1*gctT*atcL:1*ctcK*agtV*ggcS*ctcT*aagD:2*caaR*gacE*gccF*ttaV*tatF*gaaN:1*gacE:2*atgV*caaY*gctS*ggtN*aacT*cagD*aacI*tcaP*tcgT*cctA:3*ctcG*tccT*attM*atcL:2*gcgV:1*tccH*tctL*gctS*atgT*gacQ:1*ggtS*gacT*tctT*tttM*aatT:1*ttcY:1*attA:1*gggN*ccaM*aatR:2+S*cacE*gctS*gagG*attL:1*cgtK*tctA*ctcI*aatT*gacE:2*tctD:2*gctG*gacN*gttL*ttaF*ttaS:1*tctV:1*ggtA*attV*gtgL:1*cttD*gttR:1*tcaD:1*gcaV*gagQ-gct*gatE*caaI*ttcL*ataV*gctE*actG:1*agaK*gctD*actL:1*gagH*gaaM*tcgV:1*gaaL:2*aaaE:1*ttgM:3*agtH+L:1*gagK*ggcL:1*gtgI*gaaH:1*aagR:2*gtgR:1*atgI*gccT:2*ttgI*gcaN:3*aaaV*cttI:1*gatS:1*accM:1*ttgV*actV:1*gacV*ttaF*gccR*ccaD*agaP*tctG*aatR*cttK*ttgS*aacP*agcV*aacS*gttS*gcgD*aacE:2*gacY*cttF*gtgL*gaaD*actK:2*cagA:1*gcgT*gccQ:1*tccA*attV*tcaE:2*tcaA*tccD*cagL:1*actE:1*ctaM*ctcS*tcgT*aaaR*gatH*caaK*gacL*ccaE*tctV*atcQ*tcaE*caaG*atcG*gacS*tttL:1*attV:1*aaaE:2*gttI:1*gctC:1*attS:1*gttI*accI:1*tccA*attV*gaaK:2*attL*aaaE:1*gctC:1*gctE*tcaA:5*gcaS*aagR*aacG:3*attL:1*aagR*actA*gaaL*tacF:3*cacN:10*gctS:1*tctQ:2*cttY*tccA:1*aagG:3*gaaR*actM:5*tctA:1*aatT*aaaN:1*catS:1*cagE:5*catN:3*gctS*tcaA:1*gttA:3*gctS*gctS:4*gctS*acgD*tttL*ttgM:1*aagQ*actS*caaH*gacL:3*actE:2*aagR*aacK:3*gccS:1*agaK*agtM:2*aatT*caaK:1*caaR*gacS+L*attL:2*cagD*agaE:2-ggcggtagtggtgag*aatK*gatT:4*aaaQ:2*aatA:3*aaaR:1*gtgA:1*atgL:10*aatS:2*atcS*gcaQ:10*tatF*ggcS:3*gggE*gacE:1 +QBLI01000035.1:1-51373 miniprot mRNA 23266 24627 863 - . ID=BC000018;Rank=1;Identity=0.4311;Positive=0.6455;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +QBLI01000035.1:1-51373 miniprot CDS 23266 24627 863 - 0 Parent=BC000018;Rank=1;Identity=0.4311;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 671 0 671 - QBLI01000035.1:1-51373 51373 23256 26385 825 2121 0 AS:i:1065 ms:i:1065 np:i:398 fs:i:0 st:i:0 da:i:6 do:i:3 cg:Z:94M2I6M10I3M2I75M7I2M1I86M34I14M1I9M9I9M1I24M6I19M3D8M1I21M2I39M22D45M7D12M1D25M1I15M1I13M2D12M1I5M2I21M1D4M1260N7M3I19M cs:Z:*aagM*acgS*atcR*tctE:1*tctQ*gatT:1*cagE*actK*gccS*cttI*cgtK:2*acgL:1*gctG*gatG*gagD*actV:20*ctcK*tcgT:1*tggF:1*gctL:2*attL*ttgQ:1*attY*cagA*aatT:2*gttL:6*atcV*accL*atcL:8*caaP:3*gcaV:2*tacI:2*ttgI*aacD:2*gatE*ggtS:1*gggS:1+IF:1*ataV*caaD*ggtS:1*ggaD+GSKYGSSRMR*ttcY*aagI:1+DE:1*ggtA*agaK*ttgA:1*aaaG:3*accF*tacF:1*atcV*agaE:5*cggK*aatS:1*cgtK:4*accI:6*atcV:3*actS:1*caaS*aacD:1*gatN:4*gccT*atcV*atgL:3*tccN:1*caaE:1*tcaV*cttA:1*gatN:2*agaK:4*acaG:2*catN*gatR:1+SGFGSFS*agaS*tctG+L*gaaD:1*aaaR*attG*tcaA*gcaV:1*gtcI:3*actS:1*tctT:3*tatF:2*atcV:3*ttgM*aggK:1*attL:1*cgtK*tccQ*acaM:1*tctD*gatY:3*gagS*ccaG:1*aagF*cagD:2*gagT*tcgE:1*catC:3*ttcN:3*gctF:2*tccN*tccT*attV*cgcQ*tacP:1*actK*tctN:5*agaT:2*gagS*acaD*gctP*cctM*aacS*ctcR*atgI*gttI*aagD:2+DDAEVNDVVFEKRSKSSVSSLPSRTPTRENLRDP:1*tccP:1*gaaH:1*gagA*actP:1*agaV*ccaA*gaaR*tctP*cgtA*cagE+V*acaR*aatS:2*gaaQ:1*attA*tctA*gagP+VVPIATGVV*cagM:1*actV:1*tccT*cagG*ttcY*cagS:1+P:1*attL*gctQ*gcaV*caaV:3*gtcA*gatE:3*aatI*cagP*caaS*tctT:1*tttA*gagP*gctT*gagR*caaS*attV+VASHDD*cgtL:1*caaI*caaE*gagQ*cgtQ*gatK*gcaQ:1*gctQ*ctcI:6*gaaL:1-caaagaatt:1*ttcE*gaaH:1*cagK:1*gtcL:1+L:5*cgtA*attL*gcaL:1*gagQ*caaR*ttgI*cttQ*cgcQ*gagQ*cagE*tacM*cagE*tcaK*caaE:1+LI:1*ggcQ*cgcK*gtaT:1*gagS:1*gagQ*cgcN:1*ctgI*ctgM*gctS*ttgF*agaK*ggaN:3*caaR:2*cttV*ctaM:1*cagE:3*cagK:2*aagQ*gccK:2*actS:1*attL-acagctaccactcaatcagctactcagcaaattggctccaaggatgagttgattcaaaacttgcat:1*caaS*ataV*actK*ctaE*tggF:1*aacE:2*gagQ:15*ttgI:1*aatK:1*ttcM:2*ctcT:1*caaD*aaaD*gccL:1*tctE:1*caaE*gaaS*gccE*attV-gacaagcgtgagaaattggaa:1*gacK:3*aagS*aacA*gtgL*gaaS*ttgN*gctV:1-cta:2*gaaN*cgtN*gacN*cgtK*gctG*cgtE*ttgS*gacE*ttgR:1*aaaE*gttA:1*ggtD*ggtR*aaaS:1*gatM*gagI*attL*gacK*aagS:1+V:1*ttgS*caaS:1*agaK*gatS:1*accQ*ttgD*aaaP*ttaI*gatA*gatS*gccL*gagD+V*agaS*aacD*caaL*tcaA*tcgT*aacD:2*actN*attA:1*gctI*caaD-cacaag*aaaG*gagD:1*gaaN*gctT*ttgI*gagV:1*cagI:1*agaP*cagE+F*caaV:1*tcgL*gctL*ttcL+RC*aatP*ccaQ:1*ttgM*gaaQ*caaT*ttaI*ctaA*aagE:1*aagF*gatL*gaaR*gagC:1*gaaS*attS*ctcA*caaK:2-atg:1*gatS*gcgV*ctgI~gt1260ag:2*ctaN:2*gcaF*agaQ+EKL:7*aagQ:1*ggcS:2*gacY:3*agcD:1*gaaV +QBLI01000035.1:1-51373 miniprot mRNA 23257 26385 1065 - . ID=BC000019;Rank=1;Identity=0.3890;Positive=0.5629;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 671 +QBLI01000035.1:1-51373 miniprot CDS 24595 26385 995 - 0 Parent=BC000019;Rank=1;Identity=0.3791;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 642 +QBLI01000035.1:1-51373 miniprot CDS 23257 23334 70 - 0 Parent=BC000019;Rank=1;Identity=0.6207;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 643 671 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 228 111 211 + QBLI01000035.1:1-51373 51373 10782 11079 117 306 0 AS:i:157 ms:i:157 np:i:56 fs:i:0 st:i:0 da:i:180 do:i:9 cg:Z:29M2D12M3I56M cs:Z::1*aacD*tgtV*tcaK:1*ataE:2*tctM:1*catF*tctD*cctT*ccgE*gaaH*gcaE*tcaC*tctT*atgD*aatT*actS*tctE*aatD*gtcI*tgtS*tctD*gttK:2-gcagtt*atgI*gccS:1*ccaT*gacN:1*aatD:1*aatP:2*caaN+CYP*aagT*aagY*cgaK:1*agaS*catF:1*tccK*aagH:1*gctS:1*agcG*cttI:3*gcaI:1*gaaK*actV*actK*aaaQ*aatC:1*accN*agaT*aagS:2*gcaL*ataY:2*gagQ:1*tccL*ggtD*atgL:1*gcaP*cttS:3*gtgI:8*accA:1 +QBLI01000035.1:1-51373 miniprot mRNA 10783 11079 157 + . ID=BC000020;Rank=1;Identity=0.3824;Positive=0.5490;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 112 211 +QBLI01000035.1:1-51373 miniprot CDS 10783 11079 157 + 0 Parent=BC000020;Rank=1;Identity=0.3824;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 112 211 +##PAF 4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 212 51 212 + QBLI01000035.1:1-51373 51373 14099 16859 153 507 0 AS:i:137 ms:i:166 np:i:82 fs:i:0 st:i:0 da:i:174 do:i:0 cg:Z:14M4I19M3I31M1I21M1D18M2D8M2295N4M6I11M5D21M cs:Z::1*tccE:2*aagS*ataL:1*aagT*cttP:1*gccI:1*tatL*gaaQ+EILI:2*attN*gaaQ*ggaA*ctaT*aagR*gatN*catC*aaaA*gctG*ggtD*caaS*gatS*agtW*agtG*acgS*tacQ*atcL+FDM*cgcK*caaV*catP*ttgT:1*agaK*aagS:1*gggI*cccR*tcaC*agaK:1*cggT*acaR*aagC:6*tatF:3*ttcY:2*aacS*gttI+Y*gcaN*acaV*gatE*caaH:1*ggaR*gtcE:2*tctT*caaL:1*gcgG:1*gaaL:1*gagK*aggN*aatE:1*gaaN-atc*caaK*cttA*gtaI:1*aagN:2*tccA*acaI:3*ttgS*tctQ*gttA*ttgI*agtN*gatQ-cgcctt*cccD:1*ttcV:3*ttgF*cttK~gt2295ag*gtaA:1*caaG*ataL+EVETFP*ttaI:1*ttcY*tctT*aagS:1*attV*agtR:1*cgtE:1-cttcgccaactatat*ctcW*tccT*tcgF:2*acaR*gtcN*aaaN*ttcE:1*attA*cttV:1*tccD*agcI*tccY*tttY:3*ctgV +QBLI01000035.1:1-51373 miniprot mRNA 14100 16862 166 + . ID=BC000021;Rank=1;Identity=0.3018;Positive=0.4852;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 52 212 +QBLI01000035.1:1-51373 miniprot CDS 14100 14441 141 + 0 Parent=BC000021;Rank=1;Identity=0.3197;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 52 170 +QBLI01000035.1:1-51373 miniprot CDS 16737 16862 25 + 0 Parent=BC000021;Rank=1;Identity=0.2553;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 171 212 +QBLI01000035.1:1-51373 miniprot stop_codon 16860 16862 0 + 0 Parent=BC000021;Rank=1 +##PAF 1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1064 0 1064 - QBLI01000035.1:1-51373 51373 23259 26394 1773 3210 0 AS:i:2844 ms:i:2844 np:i:773 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:101M1D163M2D41M1D6M12I259M9I24M3I159M1I230M2D56M cs:Z:*gtgM*atgA*tccT*aagL*acgS*atcK*tctA:1*tctA*gatE:1*cagT*actV*gccH*cttI*cgtK:3*tctN*gctP*gatE:10*gcgQ:2*gttI:4*cacN*aagH:1*tcaG*ctcR*tcgA:3*gctG*ttaM:2*ttgQ:1*attL:6*ttaV:15*caaP:1*gctV:1*gcaR:1*ggtA*tacQ*aggQ:1*ttgR*aacS:1*atcL*gatE*ggtS:1*gggA:1*tctG*ataA*caaH*ggtA-gat:1*ttcR*aagH*ggcT:1*ggtA*agaK:2*aaaY:3*accG*tacL:1*atcL*agaK:2*cagN:2*cggK:2*cgtP*ggtD:13*gtgR*actN*gtcI*caaD*aacD:1*gatN:3*gaaQ:2*atgV:3*tccD:3*tcaA*cttI:1*gatN*ctgF:4*ttcI:1*acaS*attL*tcaK:1*gatG*aagR*agaP*tctN:2*aaaR:4*gtcI:3*actA:6*aagR:1*atcT:1*tcgT:4*attM*tacH*cgtA*tccT:1*ggaN*tctD:7*aagR*cagS:2*gagY:3*gctQ:2*ttcL:3*gctY:4*attL*cgcK:4*ttgV:4*agaT:1*ccaS*gagQ*acaD*gctP:3*atgY-gttaag*gatG:1*tcaN*tccP*gtcE:1*gagG:1*actA:1*agaA*ccaL*gaaP*tctK:1*cagP*acaI*aatS*ttgE*tcaV*gaaA*ccaS*attL*tctA*gagT*cagG*ccaA:1*gtcT*tccA*cagP*ttcS*cagT*caaT*ccaD*attL*gctT*gcaP*caaE:1*actV-ggt:5*aatT+PQGQQQLQQQQL:2*tctQ*agtQ*tttY:1*gctE:2*attR:2*caaA*caaA*gagQ:1*gatE:1*caaE*gctL*ctcQ:3*cagM*tatL*gctH*gaaL:2*agaQ*attQ*ttgQ:2*cagE:2*gtcR*caaM:1*caaA:4*attL:3*caaA:3*gagD:3*tcaR:2*cagD:2*gtaA:6*ctgI:1*gctN:6*caaR:3*ctaM:1*cagE:3*cagR:3*gccG:2*actG:1*attL*acaQ*gctQ*accV*actI*caaA*tcaN*gctT*actN*cagL:1*attS*ggcQ:3*gagD*ttgM:1*caaR*aacS:1*catQ:2*ataV*actN*ctaS*tggL:1*aacS:2*gagD*agtV:13*aacD*ttgK:1*aatK*aagE*ttcL:1*aaaL:1*caaK*caaV:9*gacE:6*aagR:1*ctgI:1*tcgA:2*gtgI:3*ggtD:9*ttgY:2*gagD:1*gttS*aagR:1*ggtN*aaaQ*ggtK:1*gagV:1*gacE:3*ttgR:1*attL:1*gatL*ctcT*accE*ttgD:2*gatA:4*aacS*caaK*tcaG*tcgA*aacD:2*actL*attL*tttI*gctS*caaK:1*aagN*aaaR:1*atcL*gaaA*gctD:2*aacE*cagA:1*agaK*cagA*caaK*tctQ:1*gctV*ttcL+DNYANRSFD*aatD*ccaS*gaaD:1*gaaK*caaL*ttaK:1*aagA*gatE:2*gaaD:6*caaE:1*atgV:1*gatS+MEA:2*gtgK:1*ttaM*gccS*aaaL*gctN*cagH*aagN:1*aatA*gaaD*acaQ:1*attL:5*caaE:2*gttK*gatE:2*acgS:2*actN:1*ttgI*gttI:1*gctS*atcV:1*ctcE:1*ggcS*ctcA*aagE:1*ttaV:1*gacE*gccS:1*tatF:1*ttgF*gacE:10*tcgT:7*atcV:4*tccT:2*atgT:2*ggtA*gacT:3*gacN:9*cacY*gctS*gagG:2*cgtS*tctT*ctcA*aatT*gacI*ttcL*actS:1*tccV:1*gctP:3*ttaN:1*tctA:2*attL*gtgT:2*gttA*aaaR*tcaD:1*gcaT*gagV:2*caaN*ttcI:1*gctS*actY:2*gctL*actA:1*gagV*gaaA*tcgV:1*gaaD:2*aaaS*tctG*ttgV*cttQ:1*gatN*agtK:1+A*gagQ*ggcL*gaaS*gtgD:1*gagD:3*gtgK:1*atgI*gccN*ggaF:1*ttgI*gcaD:2*gagT*aaaH:2*gatS:1*accS:1*ttgV*actS:1*gacK:1*gccT:2*tctA*aatG*cttT*ttgH*aacL*agcN*aacG*gttS*gcgG*aacD:5*gaaD*actR:1*ttgM*cagA*aatR:2*gccE*tccA:1*tcaE*aagS:1*tcaN*tccA*cagK:1*actN*tcgE:3*aaaR*gatP*caaK:2*tctK*atcF:1*caaV*atcF:1*tttL*gagK:1*aacH*aaaE:2*gttL*tccA:6*aatT*tccA:1*gaaA*cttA:5*attS*gctD*tcaC:1*gagQ:4*aagQ*aacG:2*tcaA*attS:1*aagR:1*gaaA*tacF:19*cttA:2*aagN*attV:12*aaaH*tctT*catP:7*catN:3*gctS:2*gttA:10*acgS*tttY*ttgM:5*aatR:2*actG:3*aacA:16*actS*cagK*agaK*agcQ*aacQ-ggcggt*agtK*ggtE:10*aatP:1*gaaV*aacL:2*gtgT:13*gccS:1*atcN*gcaS:11*ggcS:1*agaV:1*gggD*gacE*agtD*agcE:1 +QBLI01000035.1:1-51373 miniprot mRNA 23260 26394 2844 - . ID=BC000022;Rank=1;Identity=0.5523;Positive=0.7224;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +QBLI01000035.1:1-51373 miniprot CDS 23260 26394 2844 - 0 Parent=BC000022;Rank=1;Identity=0.5523;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +##PAF 4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1053 0 1046 - QBLI01000035.1:1-51373 51373 23259 26391 1623 3168 0 AS:i:2570 ms:i:2570 np:i:744 fs:i:0 st:i:0 da:i:0 do:i:6 cg:Z:99M4I201M1I7M1D7M4I265M1I39M5D134M1I39M2D33M1I153M2D57M cs:Z::2*aagS*acgN*atcR*tctA:1*tctV:2*cagN*actV*gccN*cttI*cgtK:3*tctN*gctT:2*actS:14*tggH:2*aagR:2*ctcK*tcgA:3*gctG*ttaI*aagR:1*ttgQ:1*attL:1*aatT:1*gaaD:1*cagM*ttaV:15*caaP:3*gcaR*gaaD*ggtA*tacQ*aggA:1*ttgV:1*tggF:1*gatQ*ggtS:1*gggA:1*tctG*ataN*caaN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aaaS:3*accR*tacF:1*atcM*agaQ:2*cagT:2*cggK*aatY:1*cgtP*ggtA:3*accM:4*gagD:4*gtgR:2*caaN*aacD:1*gatN:7*gatN:2*tccT:3*tcaG*cttV:2*ctgF:3*attV:1*gcaS*acaS*attL*tcaQ*catR*gatS*aagR*agaS*tctN:5*gcaS:3*cttM*gttI*actA:6*aagR:1*atcV*accI:5*attM*tacH*cgtV:3*tctN:1*gatE:3*ccaS:1*aagR:2*tacF*gagY:3*gctD:2*ttcR*gaaD:2*gctY:3*tccT*attL*cgcK:2*actR:1*ttgI*atcV:3*agaQ:1*ccaG*gagK*acaN*gctP:3*atgL*gttE*aagE*gatE*gaaD*tcaG*tccP*gtcG*gaaL*gagP*gagQ*actR:1*agaK*ccaS*gaaV*tctA*cgtE*cagT*acaR*aatS*ttgV:1*gaaP*ccaE*attP*tctA*gagP*cagL:1*actV*gtcA*tccT*cagP*ttcT*cagP*caaS+I:1*attA*gctE:3*actI-ggt*gtcE*gatN:3*aatQ*cagD+ALLA:1*tctQ*agtQ*tttY*gagD:3*attE:2*caaR:1*gagQ*cgtQ*gatA*gcaM*caaE*gctE*ctcE*caaR*caaI*caaR:1*tatM*gctQ*gaaM:2*agaQ*attQ*ttgQ:2*cagM:2*gtcR:2*caaM:1*cagA:1*cgtQ*attR:5*cttM*cgcA*gagD:1*tacM*cagA*tcaR*caaH:4*gtaM:6*ctgI:7*gagD:4*ctaM:1*cagE:3*cagG:6*actA:1*attL*acaN*gctQ*accL*actQ:1*tcaT:1*actH:1*caaS*attA*ggcQ*tccA:2*gagD:2*caaE*aacS:1*catQ*gagQ:3*ctaM:1*aagR*aacQ:3*agtT:3*ctcR:2*caaS*ttgM:1*cagE:1*cacY:1*aacA:2*aatK:1*ttcL:1*aaaA*ctcT:7*caaK:3*gacE:1*cgtA:4*aagR:1*ctgM*aaaR*tcgH:2*gtgI:3*ggtD:2*cgtK:6*ttgY:2*gagD*aaaR*gttA:3*aaaN*ggtK*gatE*gagD*attV*gacE*aagR:2*ttgR*caaE*attL:1*gatM*ctcA*accQ*ttgD:2*gatA:1*gccK*gagD:1*aacS*caaT*tcaG*tcgA*aacD:2*actL*attL*tttL*gctS*caaK:1*aagN*aaaR:1*atcL*gaaS*gctE:3*cagA:1*agaK*cagM*caaK*tctQ*tcgR:1*ttcL*aatD*ccaE*gaaR*ttgG*gaaD*caaD*ttaS+D:1*aagL*gatR*aagR*gatL:2*ttaK:2*ctcE*caaY*caaE*acaA*atgL*gacN*gatE:1*ctgF*gtgN*gaaS:2*aaaL*gctH:1*aagE*gagQ*aatQ*gaaG*acaN*gctN*attS*gaaN*gagG*cagG*atcS*gacT*caaP-gttttggttgatcac:1*acgA*aagA:1*actH:1*ttgI*gttI:2*atcL:1*ctcE:2*ctcS*aagQ:1*ttaV:4*tatF:2*gacE:10*tcgT:6*attV:5*tccE:2*atgS*gacA:1*ggtA*gacT:3*gacN:1*atcL*attA:1*gggE*ccaT*aatD:2*cacY:4*cgtK*tctT*ctcV:1*gacI*ttcY*actS*tctT*tccA*attV*gctE*gacN:2*ttaS:4*attL*gtgT:2*gttA:1*tcaD:2*gagS:2*caaA*ttcL*ataV*gctN*actF:2*gctE*actS:2*gaaA*tcgT:1*gaaR*ttcN:1*aaaI*tctG:3*gatE*agtI*ttaI:1*ggcD+F*gaaP*gtgL*gaaD*gagD:1*actM*gatE*gtgR:1*atgI*gccT*ggaL:1*ttgM*gcaN:2*gagT*aaaA:2*gatH:2*caaK:1*actA:1*gacR*ttaM:2*agaK*tctT:1*cttI*ttgN*aacM:1-aacgtt*gcgG*aacD:6*actR:1*ttgM*cagA*aatK:2*gccD*tccA:1*tcaA*aagA:2*tccA*cagK:1*actG*tcgD:2*tcgK*aaaF*gatN:1+S:2*tctL*atcK:1*caaT*atcT:1*tttL*gagQ*attL*aacH*aaaE:1*attA*gttI*tccQ:2*attQ:2*accI:1*tccA:1*gaaA*cttA:2*aaaR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aaaR:1*tcaT*attS:1*aagR*actA*gaaQ*tacF:3*cacN:1*cgtK:13*cttA:2*aagN:4*actK:3*gttT:1*tctR*gggR*aatT*aaaS*tctG*catL:1*cagE:5*catN*gagN:5*gttA:12*ttgM:5*aatK:2*actE*gcgC:2*aacV:6*agtN:2*aatK:3*gacE:2*actN*cagK*agaK*agcF-aacggc:1*agtE*ggtL*gagD*aatE*gatK:3*tcaA*aaaA:1*acaS*aatK:2*aacF:2*gtgT:13*gccD:1*atcQ*gcaG:6*gaaQ*attM:2*tatV*ggcA:1*agaL*gacH*gggQ:1*agtE*agcE:1 +QBLI01000035.1:1-51373 miniprot mRNA 23260 26391 2570 - . ID=BC000023;Rank=1;Identity=0.5123;Positive=0.7045;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +QBLI01000035.1:1-51373 miniprot CDS 23260 26391 2570 - 0 Parent=BC000023;Rank=1;Identity=0.5123;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +##PAF 4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1054 0 1047 - QBLI01000035.1:1-51373 51373 23259 26391 1620 3171 0 AS:i:2556 ms:i:2556 np:i:744 fs:i:0 st:i:0 da:i:0 do:i:6 cg:Z:99M4I201M1I7M1D7M4I265M1I39M5D134M1I39M2D18M1I15M1I153M2D57M cs:Z::2*aagS*acgN*atcR*tctA:1*tctV:2*cagN*actV*gccN*cttI*cgtK:3*tctN*gctT:2*actS:14*tggH:2*aagR:2*ctcK*tcgA:3*gctG*ttaI*aagR:1*ttgQ:1*attL:1*aatT:1*gaaD:1*cagM*ttaV:15*caaP:3*gcaR*gaaD*ggtA*tacQ*aggA:1*ttgV:1*tggF:1*gatQ*ggtS:1*gggA:1*tctG*ataN*caaN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aaaS:3*accR*tacF:1*atcM*agaQ:2*cagT:2*cggK*aatY:1*cgtP*ggtA:3*accM:4*gagD:4*gtgR:2*caaN*aacD:1*gatN:7*gatN:2*tccT:3*tcaG*cttV:2*ctgF:3*attV:1*gcaS*acaS*attL*tcaQ*catR*gatS*aagR*agaS*tctN:5*gcaS:3*cttM*gttI*actA:6*aagR:1*atcV*accI:5*attM*tacH*cgtV:3*tctN:1*gatE:3*ccaS:1*aagR:2*tacF*gagY:3*gctD:2*ttcR*gaaD:2*gctY:3*tccT*attL*cgcK:2*actR:1*ttgI*atcV:3*agaQ:1*ccaG*gagK*acaN*gctP:3*atgL*gttE*aagE*gatE*gaaD*tcaG*tccP*gtcG*gaaL*gagP*gagQ*actR:1*agaK*ccaS*gaaV*tctA*cgtE*cagT*acaR*aatS*ttgV:1*gaaP*ccaE*attP*tctA*gagP*cagL:1*actV*gtcA*tccT*cagP*ttcT*cagP*caaS+I:1*attA*gctE:3*actI-ggt*gtcE*gatN:3*aatQ*cagD+ALLA:1*tctQ*agtQ*tttY*gagD:3*attE:2*caaR:1*gagQ*cgtQ*gatA*gcaM*caaE*gctE*ctcE*caaR*caaI*caaR:1*tatM*gctQ*gaaM:2*agaQ*attQ*ttgQ:2*cagM:2*gtcR:2*caaM:1*cagA:1*cgtQ*attR:5*cttM*cgcA*gagD:1*tacM*cagA*tcaR*caaH:4*gtaM:6*ctgI:7*gagD:4*ctaM:1*cagE:3*cagG:6*actA:1*attL*acaN*gctQ*accL*actQ:1*tcaT:1*actH:1*caaS*attA*ggcQ*tccA:2*gagD:2*caaE*aacS:1*catQ*gagQ:3*ctaM:1*aagR*aacQ:3*agtT:3*ctcR:2*caaS*ttgM:1*cagE:1*cacY:1*aacA:2*aatK:1*ttcL:1*aaaA*ctcT:7*caaK:3*gacE:1*cgtA:2*ttgM:1*aagR:1*ctgM*aaaR*tcgH:2*gtgI:3*ggtD:2*cgtK:6*ttgY:2*gagD*aaaR*gttA:3*aaaN*ggtK*gatE*gagD*attV*gacE*aagR:2*ttgR*caaE*attL:1*gatM*ctcA*accQ*ttgD:2*gatA:1*gccK*gagD:1*aacS*caaT*tcaG*tcgA*aacD:2*actL*attL*tttL*gctS*caaK:1*aagN*aaaR:1*atcL*gaaS*gctE:3*cagA:1*agaK*cagM*caaK*tctQ*tcgR:1*ttcL*aatD*ccaE*gaaR*ttgG*gaaD*caaD*ttaS+D:1*aagL*gatR*aagR*gatL:2*ttaK:2*ctcE*caaY*caaE*acaA*atgL*gacN*gatE:1*ctgF*gtgN*gaaS:2*aaaL*gctH:1*aagE*gagQ*aatQ*gaaG*acaN*gctN*attS*gaaN*gagG*cagG*atcS*gacT*caaP-gttttggttgatcac:1*acgA*aagA:1*actH:1*ttgI*gttI:2*atcL:1*ctcE:2*ctcS*aagQ:1*ttaV:4*tatF:2*gacE:10*tcgT:6*attV:5*tccE:2*atgS*gacA:1*ggtA*gacT:3*gacN:1*atcL*attA:1*gggE*ccaT*aatD:2*cacY:4*cgtK*tctT*ctcV:1*gacI*ttcY*actS*tctT*tccA*attV*gctE*gacN:2*ttaS:4*attL*gtgT:2*gttA:1*tcaD:2*gagS:2*caaA*ttcL*ataV*gctN*actF:2*gctE*actS:2*gaaA*tcgT:1*gaaR*ttcN:1*aaaI*tctG:3*gatE*agtI*ttaI:1*ggcD+F*gaaP*gtgL*gaaD*gagD:1*actM*gatE*gtgR:1*atgI*gccT*ggaL:1*ttgM*gcaN:2*gagT*aaaA:2*gatH:2*caaK:1*actA:1*gacR*ttaM:2*agaK*tctT:1*cttI*ttgN*aacM:1-aacgtt*gcgG*aacD:6*actR:1*ttgM*cagA*aatK:2*gccD*tccA:1+A*tcaA*aagA:2*tccA*cagK:1*actG*tcgD:2*tcgK*aaaF*gatN:1+S:2*tctL*atcK:1*caaT*atcT:1*tttL*gagQ*attL*aacH*aaaE:1*attA*gttI*tccQ:2*attQ:2*accI:1*tccA:1*gaaA*cttA:2*aaaR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aaaR:1*tcaT*attS:1*aagR*actA*gaaQ*tacF:3*cacN:1*cgtK:13*cttA:2*aagN:4*actK:3*gttT:1*tctR*gggR*aatT*aaaS*tctG*catL:1*cagE:5*catN*gagN:5*gttA:12*ttgM:5*aatK:2*actE*gcgC:2*aacV:6*agtN:2*aatK:3*gacE:2*actN*cagK*agaK*agcF-aacggc:1*agtE*ggtL*gagD*aatE*gatK:3*tcaA*aaaA:1*acaS*aatK:2*aacF:2*gtgT:13*gccD:1*atcQ*gcaG:6*gaaQ*attM:2*tatV*ggcA:1*agaL*gacH*gggQ:1*agtE*agcE:1 +QBLI01000035.1:1-51373 miniprot mRNA 23260 26391 2556 - . ID=BC000024;Rank=1;Identity=0.5109;Positive=0.7039;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +QBLI01000035.1:1-51373 miniprot CDS 23260 26391 2556 - 0 Parent=BC000024;Rank=1;Identity=0.5109;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123585.1_orf_scan.tsv b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_orf_scan.tsv new file mode 100644 index 00000000..fb25988e --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123585.1_orf_scan.tsv @@ -0,0 +1,8 @@ +seg strand wstart wend aa pfam best_ref +s17 + 10579 11088 170 Homeobox_KN:27:136-165,Homeodomain:53:112-167 460523_cbs-4732_MATog_MATa-MATalpha_MATa1:58 +s30 + 15604 15942 113 MATALPHA2:18 +s173 + 15941 16048 36 Homeobox_KN:19:13-35,Homeodomain:17:8-35 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha2:18 +s308 + 13923 14624 234 MATalpha_HMGbox:33:92-173 MATalpha1:62 +s550 - 9116 9634 173 MATA:33 +s628 - 23254 26406 1051 ANTH:260:14-275,I_LWEQ:4:848-872,I_LWEQ:195:891-1043 sla2:1217 +s671 - 9682 10035 118 460519_cbs-7435_MATkp_MATa_MATa2:16 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.a2.gff b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.a2.gff new file mode 100644 index 00000000..879555f8 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.a2.gff @@ -0,0 +1 @@ +##gff-version 3 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.fa b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.fa new file mode 100644 index 00000000..446f885a --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.fa @@ -0,0 +1,735 @@ +>QBLJ01000026.1:100869-152175 Barnettozyma californica strain UCD89 NODE_26_length_152175_cov_69.010151, whole genome shotgun sequence +AAAGTACTACTACGGTCACAGAGCCTGCTAAGAAGACCATCTGGGAAACGGTGAAACATGAAGTGAACCA +TTACTGGGAAGGAACTAAACTTTTGGGATTTGAGATCAAAGTCTCTTTCAAGTTGGCGTTGAAGTCTGCT +GCTGGTTACGAGTTGACCAGAAGGGAGAATAAACAGTTACAAAGAACTACACAAGATATCGTTAGACTTG +TTCCATTTTCGATGTTTGTGATTGTCCCATTCGCTGAACTTCTGTTGCCAGTCGCCTTGAAGATGTTCCC +AAACCTGTTGCCATCAACATATGAATCTTCGGCAGACAAGGAAAAAAAGCGTTCAAAGCTCCTAAAGACC +AGAACCAGTGTGTCTCAACTGCTGAAGGATTCTATCCAGATGAAGTTTCCAAGTGCTCTTACTGAGGAAC +AAAAAGCGGATTTCAAAGACTTCTTGGTCAAGATTAGATCGTCTTCAGAGCAGCCATCTAGAGAACAGCT +ACTTAGAGTAGCCAAACTGTTCAAAGATGACACTGTCTTGGATAACCTACAAAGATCTCAGCTGGTTGCT +GTGGCAAAATACATTAATCTTCAACCAATCGGCACCAATCAAATGCTTACATTCAGAATCAGAAACAAAC +TGCTGAATATCAAGAGAGATGACAAAGCCATTTACTATGAGGGTATTGATAGCTTGAGCACAGTTGAGCT +GCAAGCTGCATGCAGCTCCAGAGGTATCAGGGTCAGTGGTGTCCCAGTGAGCGAATTGAAACGCGATCTT +ACTGCTTGGTTAAACTTGAGATTAAATGAGAGAATCCCATCCACCCTGCTTGTGTTGTCCAACGCATACA +CTTACGGTGATTTGGACTCTGATGAAACCATTTATGATGCACTTCAAGCGGTGCTAAGTTCGATGCCTGA +AGAAGTGTACCATGTTGCTGAGGCTGATGTTGTCGAAGAGGTTAGTAACAAACAAAGATTAGAACTGCTG +AAGGAACAGCAAGAGCTGATTGAGTCTGAAACCAAGCAAGATACTGATGATGGTATTATCATTCAAGTTC +AAGATAAGAAAAACTTGGACGATGATGAAGTGCATGAATCTAAAGAGTCTACAGAAGCCAGCAGCGAGAA +AGCAGGAGAGCCAAAGGACTCAAAGAAGGATTAGTCCTCCATAATATACATAGATCCTGTGTGTTTTTTT +TTGAACCATTTGTAGTGCGTTGGAAGTTCATGTGTCATGACGTGATGATCCTTACGATACCTGTCACTAC +ATAATGCAGAATAAAAGAGAGATAAATAAAACGATTTGAATAGCATCATTCATGATCAATAAGATTATAT +CAAGAAGGCAGCGATACCAATGGCAACTGCAGCACTTGGAGCGAATTTGCCAACACCGTTATCAGATTGA +ACAGCAACAGTTGGGGTAGCAGTGGCCTGAACCTGGCCATCAGAAATTTGGGAAACCACAGGGGTTTCTG +CAGCCGACGATTCAGAAGCTTCAGTGGCTGTATCCTCTGCATCATCGGTATAATCCTCATCGTAATCATC +AGTATAGTCCTCATCGTAATCATCAGTATAATCCTCATCGTCCTCTCCCGTACCATCGTCTTCGTCGGCA +GCATCGTCTTCTCCCGCAGCATCAGTAGCAGCAGCAGCATCGGTAGTAGTGGCAGCATCAGCCGCAGTAG +TAGTAGCTGGAACTGCACCTTCACTTTGGGCGTCCGTAGCTTGGGAAACGCCAAGGACAGCTAGGGACAA +CAAGGAGATTAAAGAAGTGAGTTGCATTATTAGTGAATGAATACGCTCTTGTACTGTTCGAAAGAAATAT +ATTAAAGGTTATGCAGAGACCTCTTGGGCTTTTATATCTTTGGAAATGTGACACCTCTCCCTCAAGATTT +CTTAATTGCACAGCTCTGGAATCTGTTCTGAGTGTAGTTCATAGCAATTCATACCAGGATTATACTAAAA +CCTTAACCCAGAATGGGCAAGTAAGTAGGCTTGGTTGTGGCTCATGAAGTTAATTCTAGAAATGAAATCC +CCGTTCTAGATGACCCCCTTGTAGATGGTGTCAGTTGCATTATTTTTTAGCTTACTATCCCCCAATCTAG +TTCAAATTACTAACATAACTAATCGTAAGACATCTGATTATTGACCACTTTTCAAAACAATACTTGATAG +AATGGCTGTACCCAAGTTATAAATAGCGTGATCGGGCTGTGTCAAATAGTATACAATTATTGTTCCGTCG +AACAGGGATCTTCATCTCCGCACTTTGCTGTGCACTTTTTGATAGAGACACGATCGAGATTTCACCATTA +AACCCTATACAGCCAAAGAACTATATCAACAATTAAAATATATTCTAGGTAGCTCTATAATAAATAATCA +ATCCTTTCCGACAAGTAGGGATAGGACACTGTCAAAGAATTTGGCAATTCCTTTCTTCTCACCTTTGAGC +CTCTTCATTTCCTCCAGTTCTTGGTAGTATTGAGCCTCATGTTCTTCAATCTCTTGCAACCCAGACACCA +GATCTACTTCCAGAGGGTTTTTCCACTTACATCTCTTCAAGACCTTCCAGAAACTATATAGACCAATACA +GACAAAGATGGCCAGATAGTTGAACAGGAAGGCTGAAACTTCCCAGTGTCCTTTGATGAAGACAGTGTAA +CCGTTGACCCACATCATAATGAAAGGACAAGTGAAGCCAAAAATACTAGTATATGGTTGCCACCAACTCT +TGAAGGTGAATTCATCTCTGTTAATACCTTGCGCCAATACCGCTTTGTAGAAAAAGTAGTAAGTGACCAT +CATCAAACAAAAATTGATCAGTTGCGACGCAGTGATCAGATTTATTAACCAATTTAAGACAACAGCGGAG +TTGTTATTCAATTGCAAGAATGACAATAGTGCCCATGCCAATGAAACAAGGACAGCGAGGATTGGGACAC +CCTGCTTGTTACAATATGTGAAGATAAATGGAGCTTTTCCTTCGAGAGCCATACCATACAGTGTTCTCGA +TGAACAATAAGTATAGGCATTCCCTGCGCTAAAAGCTGCAACAACCAACGCACCATTAACAATATCTGGC +AATACTTTAATTCCCATATTGTTCATTGCAATGACGTAAGGACTGGAAGCAGCACCTGGTCTTGACTGAT +TAATGGCTGCAGTTAAATCAGCGTCATCACTGGCAACTAAAATACCAACACATAGAGCACCTCCGATAAA +CAAGAAGGTCAATCTATAGAATATCTGCTTGAATGCTCTTGGAAGAGTCGATGACTTTGGAAGTACAGTT +TCACCAGCAACCATGGAGAGATAGTCTGGTCCTGCTATGGTGAAAGATGCCTGTGTGAGGCATGTAATGA +AGGCTTGGAAATATCCTAATGAACCAGTCGACCAGTATGGCTTAAATGCACCCGGATTCTTCCAGAATTG +GAAACCAAATGCATGATGCTGTGGGTTACCACCAACCATGGTGATAAAAGTAAACAAAATTAAACCAACA +GCCAGCAAAACTTTACCAATAGCCAACCAAAACTCAGTCTCGCCATAAATTCTAACAGCAAAAATTGAAA +TCAAGAAATAAATAACAATCTGCACTATCAGGGTGATCACAGGGGAATAATCGTCCCTCCAGTAATGAAT +AATTGTGTTCACGGCGACAACTTCAAATGGAATCAAGACACATTCCAGGAACCAGAAATTCCAGCCAGCC +ATAAATTCAAGAGCATCGTCACAGCAACGGCCAGCCATTCTTATAAAGGGGGAGTTTATCGGTAGATAGC +ACACCATTTCTGCTGTAGAGACAGTGATACATAAAATTGGAATACACCAAACGGTGAATCCAATTAATAA +GAACCCAGGACCAGCCTTTTGTAGTGCACCACCAATTGCAACAAAAAGAGCAGTACCAATGACACCAGAG +ATACCAATAAGTTGCACATGTCTGTTCTTTAGTTTTCTGTGGGTCTGTGGAACCAAAGTAGAGGCATCAC +TGATCGATTGTGTGTCGTTTATAGGGAACTTGTAGTCATCACTAACATGATGGTGATGTTCACCATGGGT +TGTGTGCAATTGCGTGTGTGCACGTTTACTTGCTGGAAGAGGTATGAAAACATCCTTCAGTAACTGTCCT +GTTGACTTCAACATTTGGTCGCTTAGCAGGAAAAAGAGTGTGTAATATGTACTAGTGAAAAGTTTTCAAG +GGTGCTGTATAACCCCAGAGAAAAAAATTGTTGACAGGTGGGGCGTGCTGCAGGCTGCAATAAGTAAGCT +TGTGAGTTTTCGTCGTAGAACTATTGAGCTGAACGAACGAAGAATAAGTAGGATATTTATTCTGCACAAA +CGAATGTATGCTAATTGCAATTCAGAAACTAGGATAACTGCTTGTAAATAATAGAATTCGGCTCATTGTG +GTCATTTGTTCCTGCCGAATGGGCGGAAAATTCCACATGTGATATACAAAAAAACAATGACCAAAAGCGG +AAATTATCCAGTCTAGTATGCGGTTAGAAGTCCTGTTTGATGAATTCTGTCCTGGGTAACCCGCTTGACG +GATGACATCATACCGCGGCTCAAAACAATAAAGATATATGGATCCTAGATGCAGTTACAAAATTTTTAAT +TCCCATATCTAAATGTATTGTAATGTATACTCCTTCAAAATAAATGGAGCACACCATGAATTTAAACTAA +ATGTATCCTGGCTCAGTATAGTAAAAAAAAACCAAATCTCTATAAACCCTTAATCAAATGCAACCGGCTA +TAACAGTTCAGGATATCAATGCAACTTAAAACAAAAAACGATCAAAACAAATATCAAAATCAAACCATAA +TGAAGTAAAGAAAAAAAGGACCCAGTTGTTGTGGATCAAACAACAGTATTGAAAGTGAATGAAGATGGGT +CTTCATTGAACTAAAAAGACGGAAAAAAGGCAAAGCAAAGCAATATAAGCACTCTAAAAGATATTGAACT +CACCAATAGCCATACCAGTACCAACCATCTCACTGGCCATACCAGTCAAGATAGCTCTTCTATTCAGGGC +TCTGACAGACTTCTTGGTGGTCTTCTTGTTGTTGGCAGATGGGTCATATCTGATAGGAAAAGTGGAGACA +TCAGGAAAAGCCAAATTGTGCTTCTCATCATGTCTGTAGTGCAAGTGCTTAGACAAAGACTTGAATGGCG +CATGTTGTTCTTCTTGAACGTTGGTGGTTTCAGCGTCTTCATCAGAAGACACAACGTCAGAAGTGTAATC +AACAAAGTCATCAATGGTGATGTTCTCGTCATTCTTCAATTCCGCTTGGGTTGGAGCAGCTAACGACTCA +TGTTTAGAAGATACAGTGGATTGACTAGAGGACTTGTGGTTTTCGTCAATAATCTTCTTGAAATTCTCCT +CCAATGACTTGAGTTCCCCAAAAGTGTCAGCAAAGTGGAAATGAGAGTTGATTGGAGAACCAGTCTCAGA +TTCAGATTCATCAGACTCAAACAAATTGTCATTCTCCATTTGCATGAACTTCACATGAGCCAATCTTGAA +ATCTCAGCGTCTTGGTTCTTTCTGAATAGAAAAGCCTTTGAGTGGCTGATGAATCTCTTGTGCTGTTGAC +TGTTTGCTGTTGGTTTGCTAAACAAGTTATCAGATTCATAGTCAGAAATAGCACTGTAGTACTCATTGTC +ATCGTCAGAATTGAACAAGTTATCGTCCTCGGATTGACCAAGGAATTCCTCCTCGCTCATGGAGACAAAG +TCATCCGGGACTCTAAAATTTGGTTGGGTGTATTCATCTTCAGATTCATCATCGGCAGGTGCTTCTGGTG +CCTGCACGACAGAAGTGTTCTGAAGTTTTCTTCTCTTGAAGTTGAGGTTATTGTATGTGTTCTCATTGTC +ATCAACGGTGGAAATAGATCTCAACACTGGCTTCCTGGAAACACTTGTGGTGTGTGTGTTGTTGATGTTG +TTGCTCATCGATGGTTGTACCAAATTCTTGGAGGTTTCTTCTGAATGTTTAGCTTTGGAAGTTAAAAAAC +GAACACGAGTGGACTTACGAGTAGATGGAGCCATAACAAAGGGTGGGTGGGTATAAGGGTTTAAAAAATT +TCTTAAAAAGGGGGGGGAGGAAAGTACTGAAAAGATTGCTACCTTCTGTTCAAAAAGTATCAAGAATTAG +TTTTAAACACTGATATAATTGAATTAAAAGGAAGTGCGAAGCAAATCAATTCGAATAGTTAAAAATTAAT +AAAGGTGTGGAAGGTTAAAAATCCCAAGAACCTAATGTGATATACACTATTTATATCAAAGATCACCAAT +TGTTTTGATGGGGATTTCTTGTTTTATATCAACCTGCTGATGAGCTTCCTTCAAAAAAAAAAAATTCTAG +AATTTCTTTTTTTTTTGCAGATAACTGTAAAATCTATGCAAGTTGGATCCATGTTGCGGCATTCCGCTTT +AGGAGCATCCCTGCCGGAGTTAACCAACTTTAGGCAGAAGCTCCACCTAGCCCCCACCTAGACTCATCTG +CAACTCCGTGCGGGATTCCAACGGCTTCTACAGTTTTGTTGGTAACTGAAGAAAAGTTTTCATAAACGAA +GGCACCAACTGGCCAATAAGGAGAGTGCAAAATAATACATATTAGCTGGAGCTTTGTCCGCACTACTCGA +GAACCTGCGCTGACTACATAGTTATGCAGTTCCTGACACACATACACACCTAGGCTCTCGTCCAAGTCCA +TTTCCATCAGCTTGTCCCCACTCAAAAGTGCTCAAAGGTTGAGAGCAATTGAGATCAACTTTCACAACTC +TCAGCTTATTTTTTAACATGATAGGGCTGTATATACCATTATGGGGTTTTGCCCCCTTAATGACTTTTGT +TTGCTATAACGTGATCATTCAGCTAATCAATCAGCTCTCCAATATGGCATCGTTAAGCACGATGAAGGAT +TAACTTCTTACTCCGTTTCTCTGGTGGGCACACACAAATACACACATACACACAGACTACTGACACATTT +TTTTTCGATTTCCACTATGTTCTGAGTATCGAGAGAGAGCACCTGTTGTTTAGGAGAGTTCTATGGTGGG +GTGTGATGATCCGTCTACCACGGATTTGTATTGTTAAGAGAGTACGAAAGAGTATGTGCGTGTATGTGTG +GGCTCGCCTGTCTTCTAGTATTAAGTATCGCACCTCCCTATGTTAGTTTTCGATCTTTCTTACAAACAAC +AAACTTGAGTTACCATTTCGGTAGTCACAAAATAGGATCACGCACTTGTGTAAAAGTCATTACCGATAGA +TAGCCTTGTTCATGTACGTTATGGCCCAGAACATCCTCTTTGAATTCCCCTCAGTCCACTGGAGTTAAGA +ACCCTTGCTGCGTGTGATTAGGATATAGCTGAATCTATCTAATGATCTCATCTACAGCTCGGTACAATTC +ACGTGGTTAGATTTTCATTGTTAGTTGTTGCTGTTCTTGTCTTTTGTCTTGTTTTTTGGCTGTGGTATGG +TTATGAGTGGTGATTAATGAACAAAAGTCGAGGTGTTTGTTGATCTGTGATCTCCAAACCCTGTGCATTA +CTCAGAACATATTTATTTATAACCGTAGCATCGAATAGAGTGGGGTTTTCATTTTGCCATTCATTTACAC +AGTTGTTTTATTTGCCTAATGTACCTCTGCCCACTTCTTGATGTTCACTCCTTTTCTTTGTTAAGCTTTG +TAGAAATATGTACTTGTCGAGGTATCAACTGAACATTAGAAAGAATGTTTAAAATTCAAAGGAAACTGTG +TAAATTGACTGTTTTCGCTTCTGATTGAATATAATCCAAGAATAGAAGACACCGCAGACGCTACAGATTA +GTGCTATTTTTGAATCGAATCTATGATCTTGACTATTACACCTTGGATCATTGAAATCGTTGTTAGAGAA +TATTGTTGAAAGCAGGACTTTATAATACTGACTTAGATGCTCCGGCTCGCATATGCTTAGACTTATGACG +CTTTATACTGTACACACATGTTCCTGGCGATAAGGAGGGTGGATATTTGATTGACCTGTTGTTGTGTGCA +GTAATCCTTCGAGAAAGATGATTCGAATGACCATTACGCAAAACACCCTCTTTTGTTGTTATTTTTTCTT +TCTTTCTGGTAGGGATTGAAATCAGCACCCACAGGCAACGTGTAGGGAACGTGTAAGGAACGTGTACTAA +ATTTCACTAGTTCTTCTATGGCTGGTTAGTCCATGTGGCCCTTGTAAACTGCAGATGACCTCAGAATCTG +TTGCATGCGTAGTGGGCTACCACCCACGAGTTCCCCAACTATTGTGTGCATTCACCAACCAACGCATGTG +GGCTGCTCGAAATGAGGGAAGTTTTTTTTTATTTTGACATGTGCCACTAAAATAAAGTTGCTTTCGAGAG +AAGCCCACTGGCACATGTGCGATCTGCCCCATGCAGTGAGACATGTGCGCAAGGCACAAGTCAAAACATG +TGGCATATAGACTATAGTAAGTATAGAGATAATGGTAGAAACAATCTACTACTACATATAGCATTTATGG +AGATAGCGAAACAATCATTGTGGGATTGCTGGGCTGTATGTAGCTTATCTTCGGGTGATACCAACTCAAA +ACCAGGAAAACCCAACAAACATTCTGATATCGCCCACTCGAATTGGAGGGGGGATGATCACAGAAGGAGA +AAAAATGATTGCTATTCAGATGCACGTAGATTAGAGAAACATGTGTTAGGATGCAGTACAACTAAGCTAA +GCCTCCTCGATCATGACACGTGAAGCTCAAGAGTAAGAACTGGAAGATGGATGCAGGATATATACAAACT +CAAGTAGAACTGCAAAAACTTAAATTTTTACACGAAGTTGACAGGTGTTGACAGCAGCAGCTCTAGTCGA +GTCGGGACGACCCATTACGGCCTTATCTCAAACATCAACATGATCTGATAAATACAGATCTCCTCTCCTT +TCTCATTGGCTGGTGATATCCTGCTAATCAGCACATTTAAAATTTGTATGTGAAACCTTTTCTTAACTAT +GGAGCTGGTTATCTGCGTTTGGCAATGTTTTTGTTTCACCGCCGTTGTGGCTTATCATCCACATAGATAA +CAATCACAACACCGCATTCGCCTTGCGCTTCTGGAAAGGGGAAAGATCTGTGACAACAGATCCCGATGAC +GTTCCCAACGAAGACTGATGCAGCAGACCGGTGATACCCGGGGATCCATTCCCATTCGCATCGCATCATC +TTGTCTGTCTCTGGAATTTGGTCCGTGACGTCGTGGTTGCGAGCTCTGAACAGTGAATTGTGGGGATGAT +AAGACCAGATAGGTTAGTTTCAATATGAGCAACATTAGCATCAATCGATACTTTGGTTGCTATCGGTATC +TTCGTAGATACTACTCTGTCGCATCTTCACATGGCCTATCTCTCGTCGGGCTTCTCTCTCCTTCATCATG +AGCAAGTTTCCATTTTCAGGCAGAGATAAGCGATGCTGTGAAAAATTTTGCGTGTATTCCTTGACCTGAC +CTGACCTGCATTTGCAAAAAAAAAAAGTAGCTGAAAGAGAATACATGCTACGGAGCAGAAGGAATAATAA +GATCCACAGTGCTGCGCGTGTCTATAACTACGTATATAGAACCACCTCTTTTTGTATAAGACCAGTTAGA +ATGAATCAGTCTGTCTAAGTATATTACAATACTGATTACATCAGCAAGGGCGAGGAGGTAGTCGCTTAGA +AGGGCAAAAGAAAAAAAATTATACCTCTCACTATTGACACCAAACCAAATCACAATACAGCACAACGAAC +ACCAAATACAATGGCTCCTGGTGACTTAAACCTTAAGAAATCATGGAATCCTGCTCTCATGAAGAACCAG +CAAAAAGTCTGGGAGAAAGAGCAGGAGGCACTAGCGGAACGAAAGAGAATCCAGGAACGCAAAGCTGAGA +TAGCAAAAGAACAGGAACTCGCTGAGCTTCGTGAATTACAATATAAGCAAACCGGAAAGAGAGTTGTCGA +TAGAGTTGATTGGATGTATGATGCTGGAGGTGTTGGAGATGGCAATAAATTGGCCTCTAATGCAAATGAA +GATTATCTTTTAGGTAAAAAGAGGGTTGATGATCTCATTGTCAATCCGAACAAGAAGAACGAATCCAAGG +CCACAGGATTTGATCGTGTGGCAGCTGGAAATAAGGGAATGCTCAGCCAAATGGAGGAGATTAATCGATC +TCGAGAGGATCCGATGGCAAGAATTCAAGAACAGAGAATGAAAAAGCTGCGAGAAGTGGCTATGGAGAGG +AAACTCAGGGAAGATGAGAGACAAAGAGAGAGATCACCTAAAAGAAGTAGAGATCATGGGCATCATAGAT +CTCATAGATCTCATCATAATCACTCTGATAAGAAGAAGTATGATTCTGATAGAAGGCATAGAGATTTTGA +AAATAGACACTCAGAAGGAAGACAAGGGAATGACAGAAGGGAGGTAGACAGAGGTTCTATAAGTAGCAAA +CATAGATATTTGCGCGATGGGGGAAGAAGTGAAAGCAGAGGCTCGGCACCTGGTTCTCAACCAGTTGATT +ATTAGGCCACTGTATTTAATGACTTAATACTGAAACGATCAAACTAGACTGAAATTTTGCAAAAGAAAAG +AAAACTTGGCGTGGCCAATCGAACACCCGGCAAATACCCTAAATACACACTGACACATACGTTCACCTCT +TCTGGATGTGGTCCAAGCCAGATATTTCCGGTAGGAAAACTATCCCCGATAACAGCACCCTCAATAAACG +GTTCGCGCCACATTCGGAGACTTCGGCGTTCGGATTTAACTTCTTTCGTATCTTTTTGGCACCCTGAATT +CGAAGAAAAAAGCAGTGGAAACTGAAATCTTTTCAATTCAGACTCAATCTCATACGACTCCATCGGTTCT +CACTATCCATTACAATGAGACTGCTGAAGTTGCTTGTCGCCACCGCAGCTGTGGCTAGGGCTGCTGATGT +TGCTGCTGCGGTTATCAACAGCGAAGATGAGGCTGCTGTTACGATCCAGGAAGATGCTGCTGCGTCCACC +ACTGAGGCACCAGAGCCAACAAGCACCTCCATCACGGAGGCTTTAATCGTAACAGAGGACCCATCTGATC +GTCGTGCTGGTAAGATCATTGACTATGACCGTGATGACAAACCCGATGAGACCGAGAAAGACGACGGAAA +CCCAGATGAACGTGAAGGTGATAAGAAGGACTCTAAAGATGGTAAAAATGACGGTAAAGTAAGAACATCT +GCCTTTGGATTCAGCGGTAAGATTGTGAAGTACGTGTACTTGGACCGTGTCCCTGGATCTAACTTGAAAG +ATGAGACAATCGTGCTGTTAACCTCCCTAAACAGAGGCTATGTGACACATGACCAAGGTTCCACTTGGGA +ACAGATTGCTCCAGAGGAGGAGATCCTCGACATTTTTGTAAACCCATACAATACAAACCACGTCTATTTG +CTATCCACCAACCAGAAGATCATCTACTCGACTGATAGAGCTGATAATTGGAAGTCCTTCAGAACCCCAG +CCAACTTCATTCCTGGTGTCAATCCTCTTCGTTTTCACGCTCGTCACTCCAACTGGTTCATCTACGTTGG +TCAGAAGGACTGTGATACATCCCACACAGGTGCCTGTCGTACTGTCACGTATAGAACACAATCATACGGT +AAGCGCTTCCAATTGTTGCAAGAACACGTTAACTCATGTGACTACATTGGCCATCTTGCCGAACCAACTC +ATGAAAGTTTGATTGTCTGTACCAGAGAGTTGAACGAGCACACTGTCCATTCCGAGGAGTTGATCTTCTC +TGAGGATTACTTTAAAACCTCATCATCTCCACTGTCCAATGTTATTGGTATGGTTCAAGCAGACGACTTC +CTTGTTGCTGCCACCTCTTCTGAGGATGGCTCGCTAAGAGCCCACGTCTCAGTTGATGGTAAGACTTGGG +CTGATGCTGAATTCCCATCCAATTTCAGAGTCGACAAGAGACAAGCGTACACAGTCCTTTCTGCCCAATC +GCATGCAATCTTCCTACACGTCACCACCAATGCCAGAACAGGCACTGAGTTTGGTGCTATCCTCAAGTCC +AACTCTAACGGTACTTCTTATGTCATGTCCCAGCAATATGTCAACAGAGACTCCTCTGGATTTGTTGACT +TTGAACAGATGGAGAACTTGGAGGGTGTCGCCATAATCAACACTGTGTCGAATCCAAACGAAGCACAGAC +AGGTTCTAAAAAGAAATTGCAATCTAAAATCACACATAATGACGGTGCTGACTGGGACTTCTTGCAACCA +CCAGCTGTTGACTCTGAAGGAAAGAAGAATAAATGTTTCGGCAAGCCACTAAAGGAATGCGCCTTGCACT +TGCATGGTTATACTGAACGTGCTGATTACAGAGACACTTTCTCCTCTGGTTCTGCGATTGGTATGATGGT +TGGTGTTGGAAATGTTGGTTCAGAGCTGGGCCAATACCTCGATGGTAATACTTACTTGACCCGTGATGGT +GGTGTTACCTGGAAAGAAGTGAAGAAAGGTGTTTACCAATGGGAGTACGGTGATCAAGGATCCATCTTGG +TCCTAGTTAACGGACAGGACTCCACTAATGTCTTGTACTACTCTTTGGATGAAGGTGACACTTGGAATGA +ATACAAGTTCACTGATGAGTTGGTTGTGGTCAACGACCTCTCCACTGTTCCATCGGATGATTCGAGAAAG +TTCCTTCTATTCACTCGTCTACCAGCCTCTTCAGGTGACAGAGCTACCGTCTATCAGATTGACTTCTCCC +AACTACTAAAGAGAAAGTGTTCCCTAGACTTGCAACACCCAGATACTGATGACTTTGATCTATGGACTCC +GCGTCACCCACATCAGGCTGACAACTGCTTGTTTGGTCATGAGGCGAAATATTACAGAAAGGTGCCAGGT +GCCGACTGTTACATCGGTGAGAAGTTGCTCAAGCCATATGAAGAGATGAGAAACTGTCCATGCACACGTC +AAGATTTCGAATGTGATTTTAACTATTACAGGGACTCTGCGGGTAATTGTAAGTTAGTTCCAGGCTACAC +TCCTCCAGACACCTCTGCTGTCTGTAAAGAATTTGGCGGTGATGAGTACTGGTTGCCAACTGGTTACAGA +AAGATTCCATTGTCAACATGTTCCGGTGGTCAGGAGTTTGACAAGGTTGAGGCTAGGCCATGTCCTGGTC +GTGAGGAAGAGTTCAACAGAAAGCACGGATTGAGAGGTTTCTCATTGGCTCTAGTCATTTTGATTCCAAT +TATTGGTGCTGGTGTTTTAATCTATGTTATCTACAACCATTATGTTGGTCGTTATGGCCAGATCAAGCTT +GGTGATGACAATGAACCAGTTGAATTTGACACTGACACCACATTCAACAGAATCTCATCAATTGTGGGCT +ATGTCGTTGTCGTAACAGTTGGTTTTATCAGCACAGTGACTGAACCTGGTATTGAGTTGATCAGGGAGAA +GCTCTTTAAGAAGAGTGTCTCTCTCGACTACAGCTCTGCTACGGCCACTGCATACTCTGATGACATCTTA +GACGAGGCCTCTTTGCATACTACCCAGCATGATGACACCGATTTGCTGAGCGATGGTGACTTGACAGAGG +CTGAGGATGATCGTTTGTGAATCCCTCTAATAGTAGCACCCCATGTCTATAGCTAAAGGAAAAAAGAAAA +TATTAAAGAAAGTTACGCCATAGGTATATTTGTAGGTCATCCTCATGTAACTTTTTCGACGGGAGTTTTG +AACATAATGACGGTTTTTTAAGGCAAACGGCTGATCATGAGATCTTCATTGTCAATAAAACCTTCTCTGC +ATGAAGATATTCTTGAACTTTTTTCGATAAAGCTCATGGAAGCCGACTAGCTTGCTTCAGACATAGTTTT +CACCTTTTGAGCAGTCGTGCTTGATTCTTATGTTGGTACAACTACTCCTGTACTTTGAAAAAGCATCCCT +GGGCCTACAAATGTGCCAAAATCGTCTACCGTCTATTGAAAAATGAGTATCCAGAATAGCCAGCACGCTC +ATTGCTGAAAATAAATTTGAAGAATAGAAATACAACCTCCTCCGATAATTCATTAGCTTATGCTGCTCCA +TCTGTGGCAGAGAATGAGACATTGTCAAATATTATCCTCAAGGCTCCACTTTCATCACTTAAAACACGTA +CTCAGCAAATATTTTGAACTTATGTATGCAAAACGTCTTTATTCTATCAATTGTATGTCTCTTTTTTGAG +ATGAATACTATCAGTCTCTCGCCCTTAACTCTGTACTCCTTACCAACTGGAAGAGTTTGGGCAGTTCATA +GAAGCTTGTCAAATTAATGCCAATCAAGAAGATGTATACATCATTACTGTCCCATCACTGTACCCAACAG +CCATGAAATCATTCTCAATCACTTTAGCATCCCTTCTATTCCTTAATACGATGTCCCTTCTCAACTCAGA +TCCCTTTGTAATTGACAGTGCATTTGGTTTCGTGATCTTCGCATTCTTTGTCTTAACATCGTCATTTTCA +TTCAAGTTCCCAACTATGTTCAAATTACCAGTTATCATACCCTTTGGTTTCCTAAAACTTGACTCATATT +CCAACTCCAATAACTCCTTATTGAGTTTATAGAACTTTATAGCTCCATTCCAAGATGCAAATACATATCT +GTCGTCAATGAATCCGACACTAGCAATTTTTCCTGATCCTGATCCCTTGTCGAGTGTAATCAATGATGAG +CGTGTAGATCCAATGGAGTGCAGCGCAATTTCTGGTTTAATCGATGTTGATATCACTGTGTTGTTTGTTA +CATCAAATGTCAACGATAGAACAGGGTCAGGAAAATGGTGTGTAGCGACAGAAACAACTTTGATTGATTG +GGAGCTTAAGTTTAGTTGAACCTGTGCAACGTGTCCAGACTCATATCCAATGAACAATATGTTCTCAACG +GTCCAAAGCAACTTCATAATGATTCCAAATTTCCCGCTCCTATTTACATCTCCATCGTCGTCTCCGCTGG +CTCTAATCTCATCAAATTCAGAACCTTTAATCTGTTTAAAGATATCCACAGATTCAAGCACTCTCCTCAG +TCTTCGATCATCCAACTCGCCATCACTTCGTAGAAATGACACATCATAAACATCGAATGCCTCAGAATCT +CTTGTATTAGGAGTTACTAACAATTGGTCAAAGATATCAACATTACTGAAATTCAAGGCATTGACGGGAA +GCTCATACACCATCTCTTCCTTCATAACACCATCAGGTCCAAATAATCTCCAGAACTTGATACAGTGATC +TCTTCCATGTGTCAGCACCAGGCCAAAGTAACTCCAATCAACTATGTCATCTACAACTCCCATCTGTCGC +ACAGACATGATTTGAGATGAATGTGCTTGGAATTGTAGTACCGGCCTATGCGTTGAAAGATCCCAAACAA +TCAGTACTCCTGATTCGTCAACAGACACTAGGTATGGTTTCAATTGGATTGCCTGAGTCTTAGCTGCTGT +AATCTGTTTGAATGTCACTAATGGTGTTTTGATGAATTGGAGGGACGTGATTGGGTGAGTGTGCTTTCTG +AGAGTGAACAATGGTGGTCGCGAGTCACTCGCTAGAAGATCACTCATGGCGGATAAGATTTGAAGCTCTG +ACAGTGGTGGAGTTCTTACAAATGTGATTGATTTTAGATTTCTGCCAGTGAAACTTTTTTTTTCAAGCTT +CTTCTTACTAAAAAATTGTGTCAACTTTGAAAATGGGCATCAAGAAAGTCATAGTGGCACTCCTCTGGGT +GTAGGTAACTCTATTACTACACTGAGATATTAGAGCGTCAATTATGCTTATCAAGGGCAGTAACTTGTCT +TCAGTTGCCCATTTTTTTGTCAACAGAGCACCTTCTCGCTAGACATTGGTCAAGCCTATGCAATAGACAT +ATAAAACTGAAAAGGCAATTAATTGTCTTTCATTTCTGTACAAGGATACTGAAGTCTGGAGTTTGAGACT +GTAAGACAATCCTGAAAACTCATCGAGGCAGAGAGACCATGCTGATCAAGCAAACCAGTTTTTTTTATGT +TATACGTGAACATCTTTTAGGCAGCCCATGCTCCCAAAATGATGCAGATATGCTCATCAACATCACTACT +GTGTTCACAGACTACAGACCAAAGATTGAACAGGTTGACAGAGGTTGACAGTCATACATTACAGACCTTC +CCACCTTCCGCAGTCCTATCCTGACTGCATTGATTTTGTCCGTGGTAAATGAGATTGTGCTCTTCGCCAC +AATAGAACAGTCGTTTCTGAAGAATTACGGAGGAGAAGGAGATAAGCTCGCAGCTATGTTATGAAATCCG +CATCAACTTATGCTAGAAAATTTGCCAGCAATTGTTGTTGGACACAGCACAGCCCCATCGCAACAAAAGA +ATGAAATTTCTCGCGGTGGAAAGCGTCAAAAAACCCCTAGAGATTTTAATTGAACAAACTACAATCAACC +TCGCCTTAATTAGACATTAACCATAACAATACAAAAGCTTTATCGATCAACAATGTACTTTCAACAACAG +ACTGTAGTCTAAATCAAGAGTGCGGCACCAACAGCAATGGCACCGACGGCAGCACCATAGGCTGGGGCAG +AGCCAACAACGGCCGCATTAGAGGAGGTTTCATTAGAGTTATTTTGGGCAGCAGCAGTAGCAACAATGGC +AGCAATGGCAGTAACAGTAGAGAACTTCATTATCGGCTAGTGTTTGTGTCTATGTGGTGGTATGTAAAAA +AGAGAAGATATGAAGACATTTTATCTACAATTTATACCCACAGGGAAAACTTCTCAGATTGCCAATCAAT +GTGGCCATAACATAAGTTTAATTAAATCTTGGAATCTGTACGAAGGGCTTAATTTAATTTCTCTGCTCAA +TTAGGGCAGCGCAATCTTGAGGCCCCACTGGACGTGACTGATTAAAGTTGCACCTGTGCAAAATTTTGAA +AAGCAATGTCTCTGTGCAGGAACTTCACCTCCTAATTAAAACAACCACAGTATCTTTTTTACCCATAAGA +GAACTGAATTTTTGCAATTCTAACAGACCAATGCGACAATGACGACTTTCTCAACTCACAGCTTATTGGA +GCAATTTATCTGTTCCTGGAACCTGCATTCGTCAGATATGCACGTCTCGCGGCATTTGATACCTAATCGG +GAAATAAGCTATGGACTTTTTGGCAATGGAACAACTTTAAAAGCTTCTCAAAGTATTTTCATTCTATCTT +TAGCAGGTAGTACTGAGTACTCAGTGAGCAATTACCACAATCCATCCGAGACACGGATATGACGGAATGA +TTTTTTCTTTTTTATTCTTTTTTGTTCGCTTTTCCATTTCTTTCTGACGATGACGTCATTTTTATTAAAA +GGGATAGAACACCTGCATAATCGTGTCTTTTTGAAGCAACGTGGAGATCTAATTTAACCACATTCCTTCA +AAAACAATCATTGCTATTGCTATGTGCGAATTCAAAGAAGACTGGGCAAACAATATTTTTTTAACTTGTT +TGAATGGCACTGATTTCACAGCACTGCCTTTTCTTGTACACAATACTGTAGCCCCACCCTACCCCCACTG +GGGTCTCGAATATCCTGCTCAGTAGCTATCAATATAGGGACAACCCCAGTGGGGAGAGAAAGAGGCTCAC +GAGTGGAACTTTAGTGTTTAAGTGCGTTAACCCAGGGCTAAATTACTCATTATCTAATTATTGAATGCCA +TACTCAAGTGTGGTATGGTGTGTGTATTGATTTTTTGTGGGATTCTTTGCGTGGCTGTGTTACAAGGCCT +AGACGTCGATGCAGCTACAAAAATAAAAAGCTGTTTTGATACGTGAATCTGCAGCAAATTGTGCGTGGAT +TTATTATCTTGAGGTGACGAGAATTTTATCAATGTGAATTCTCGATCTTCAAATTCATCACTTCTCTGGC +AACTGCAATAGCATCAGGTGGGAAACCAGCTTTCTCCGCCACCTCAATAGCATATGACTGTTCAGAAATA +CCATCTTCTAATTTTCTATTGAAATGAAGATGTCCGCTCACTCGTTCAGCTGTAATTTTCTTAAATGCGA +AGGTCTCAATTCCGGTAGTTTGATACTTCTTGATTAAACGGCTAATCTCATTACCGTAATGAGTAGAGAA +TAGGATTCTCGACTTATTTTGATCAACCAGGTGGTTAAGTGTAGCAAATGCCACTGCTGTTCCCTCTCGG +GAACTTGTACCTCTTCCAATTTCATCCAGAATGGCCAACGATCTATCTGTGGCACCATTCAATATGTAAC +TTGTCTCTACCATCTCAACCATAAATGTGCTCATATTGTTAGATAAGTCATCCGTAGAATTCACGCGACT +GAACATCTTATCTACAACACCAATCGTGGCACGAGTGGCTGGGATATACGACCCAATCTGTGCTAGAATA +ACAATAATGGCATTCTGTCTCAAAAATGTCGATTTCCCACCCATATTTGGACCAGTGATGATCCATGCAA +ACTGGTCCGAGTGAAGTGTCAGATCGTTTGAAGTGAAACGCTTCATCTCAGCATTCAACGAATCTTCAAC +AGCAAGATGTCGCCCATCAACAATTTCAAGATCTGTCGTTCCATTAACTTCGGGGCACACAAGCCCCCTC +TCATGTGCCAAAATGGCAAAAGAACTAGTCACATCAAGTGAGTCAAGAAACTTCCCAATGTTTCTTAAAT +GGATGCTTCTCTTGATGACTTTTGCTATCAGCGATTTCACAACATTATTCTCCTCCTTTCCAATTCTAAC +CCTCATCGCATCAATAGCATGCCCCAATGATGTCAACTTTGGGTAACTGTACCATCTTGTATACATACTC +TTTTGGCTCAATACACCCTCGGGAAACAATCTGCTAAGGTCATCGTATTTACTTGTCTTTGTTCCTTTGA +TGTATACTTGATATCCCAAACTTGGACTCAGCTTCAATGACACCTCTTTAAATACACCTTCAGTCTTCAA +TTTGTCCCTCAACATAATCTCGAGATCGAGACGTTGCTGCTGTAACGCATCGACTTGATCATGGAGTGAC +TTCAAAGTTTTGGAGGATTCCTTTCTGATAACATACAGGGGTGGCGAAGTTTTTGCAGTTGTTCTATTGG +CAGTTGAGTAGCTGTAGGTTTCGCTAGCTAGAGATTCTAATTCCTCTTTAGCAACTTTCTGTTGATTTTC +CAGATCAGTGAGTAGATGTTCCACATCGATATCCTTGAGAATCTCTTTAGCTAACTTCATTTCCGATTCT +GGTATTGGTGGGAGTGACTTGATAGCATTGTCATCATTGGTAAGTTTCTCATTGATAATTTCCTTGATAC +ATTCAAAGGTCTGGACTGTTGTAGCGAGTTGAACAAGGTCAAGAATACTACCCCTTCCCATTGAAAATCT +CTGAACGATTCTGCTGATATCGTGACATTCCTTCAATCTCATTACAATCTGCTTTCTAAAAATTGGCTTT +TCAAAAAATACTGTAACCATTTTCTGTCTAGACAAGATTTCATTGACATTTGTCGAAGGTGCCATTAGCC +ATTGGGAAAGGAGTCGAGCACCACCTTGTGTGGATGTCCTCCTGACACAAGACAAAAGAGAACCCTTCGC +CAACTGGTCCCTGTATGTCTTGTGTAACTCTAAGGCATCTGATGTTCTGGAATCAATTTGCATAATCATC +TCAGGATACTGTTTCTCTGGTAACTGAAGACCTATTTTCGTTTCAGGGAAGTGTTCCTCGATGTAATTCA +ACAAATTTTTCATTGCGGAAGCCTCTTTTACTGAAAGAATATCAAATGACTTTTTGATGACAGATTGGGT +GAAGTTAAACATGCCATAGAATAGGTCCAACGTCTTGTGTCGCGCTGGTAAGTTCTGGTACTTTACAAAA +TACTTATTCAATTCTATAAGTTCCTGGTACCAATCTCCGCTTTCAAGCCTGTACTCAACTAAATCTTCAC +TCAACAGAATTTCAGAAGGCTTTATCCTCGAAATGGCTGACGCTAGACCTTCAAGTGTAACTTCTTGCAC +AAACAATGTTCCAACAGATATATCAATCCATGCTAGCCCAACAGCTATGTCTGGATCTGCTGGCCTGTTA +ACGGAATTCTTGGGAATATCAATAACCAACAGATAGTTGTTTTCCTGCTCGTTCAAAAAAGCCTCATCAA +CAAGCGTACCTGGAGAGATCACTCTTGTAACACGTCTCGCAATCTTCACTGGGTCGTTATCAATTGAGGT +TTCCCTCTGAAACTGTTCTGAAACTGCGACGCCCATGTTCAAATCCTTTACAAGCACCTTAAGGTGTCTA +TCCAGTTGAGACAGGGGAAATCCAGCCATTGGTACAGTAAATTCCTTGAATGTCTTCTTAGTGAGTGTGA +TGTTCAGCCTTGGAGCAATTTCAGTAGCATGTTCAAAGTAGAGTTCATAGAAACTGCCAACCTGCGTAAT +GACGATGTAGCCCTTGTACCTGTCCATTGTTTCACGAACATTGCGCATCAGTGTGGGTAAAGAGTCAAGG +TAGAGCTTACGCTCCCATTCCTTCTTTGCATCTGGATAAATCTGTCTATTGCTCTTCAACTTGAGAATGA +CCTTTGTTGGCTCTTTGTTAGGTCTTGGACACACAGAATGGAATAGATGCGGGACTGGCAATCGTGCAGT +CCACTGTCTTCTCAGCACCAAAGCTCTTGTCCTGATCATTTGCCGTCGATATCAGTAGATATAAATGAAA +GAAGAAAGTGGTCTATGAAACTTCCACCATTCTTACAGAACGCATTGTTAAACAATAAAAACTCCTCATG +ACAGTTCGATGATCTGATCGCCATGGCATGCCCTCGGAATACCGAGTTAAAAAAATCTTTGGGTTTAGGA +CGTGTTCTTGTCCTAAATCCACCGACTACAGTTGTAGCTAAGTATGCTTCTTATATATAACAAATCATAC +TCTGTGGTTCAAGATTAATACATAACTTTCATTAACTATTTCTTCTCAAACCCAACAACTAAATCTCCAG +CAAAGTTCAAAATCTCATTGAAAATGGCCTCCTTGTTGATGATAACAACGTCCTTTTCGAGGGGCTCAAA +GTTGTAACCAATCTGCACTCTGAACTTTGTCTTTTGTAAAGTGTGTGGAACAAACAAACCACAAATCACA +CTTTCTCCACTTTCATCGATGTTGTTCTGTTTGTAAGAGAAAATTACTGAACCCTTGAACTTCAAGCAGA +GTTGTTTAATCAGTTGCGCCACTGGATCATACTTGGAGTGAAGCTCAGTAGGATATGATGAGGCGTTGTT +AACCAAGTTCTTGAAGGTCCTCTTTCCTTTTGGCAAGACACCTGAGCTCAGAGTGAGTGGAGTGAACTTC +AACTTCAAGACAAAATCATAGTCAGCAAGAGACGGTGTAAAGAGCAGCTTGATTGTATCATTTTTCACGC +CATTGTTAGTAACCAACTGCATAGCAACCTTGGACAAAGCAGTTAATCTGGTTGCAATTGGTAGCGGAAC +ACTGTTAGACCACAAAATACCAGACTTGTCAATTTTCGAAGCTACAAAGAATTGCGTTCTCATGCCCAAC +GGATCCTGCTTTCTGATGTTGTCGAAGTTAGACTTGATCTGCTGATATGACTGAACAGTAATCTTATCAG +AAAGCTTAGAAACTATCTCGCTAGCGTCTTCAATGTCGTCTTGCTTGGCACTCAAATCCAAGATTAGGGG +CTCTTCTTTCCAGTTCCAACTGGCAAGAAAGTTCAAGATCTTCAAGAACCCATTCTCCACTGATGAAGGT +GAGTCAAACTGTGCTGGGTCAACAAAAGGCTTCAATGCAATTAACTCTACCAACTCTTCAGTCAAGTGTG +GTAGAAGCAGCTGATCGTCAAGCCACTTCTTGAAAAGCCTTACTGTTGGTGAATAGAATGGGAATTTGTG +TGACACAGTAGAGATCGTTCTGGTATGAGTAATGGAACCAAGATACTTTTGGTTGAACTTCAAATAGATA +GATTCCAATTCTGATCTTTTCTCTTTTCTAGCACTTTCGATCGCTCTCAGATACAACACTTCGTCACGCT +CAGTCAATACTCTCAATCTGAACCCATAGCCTTCTGGAGACAACACGTTCAACGTTGTAATGTCGAAGTT +GTAGGGAATGGAGTCCTCGTCTTTGTTGATAAAGCTTCTGTAAACAGTTTCCTTGGTCAAAATCTCATGA +ATCTTTATCAAGAACGCAGTCTTAGTATTCTCCAATGCACTGATCTCATCAGGCCACTTCACTGATGATT +CGAACTGAATAATCAAATCATTGAAAAATTCCTTGGATGAAACAGCAAATGGGACAGGAGTCACAAGTGC +AGTAGATCTCAAAGCTGGGCCAGCAGGTAGCACTGACTTGACAGCTAATGGGAGTTCCAATTTAATTAAT +ATTCTGTAGAGCTCATCAAAACTTTTGCGAAGAGCAACAGAAGCACTGGTAGAAGTGATAGACTGCTTAG +TTGATGATGGAAGATTTGGCAGTGGCAACAAATCATTAAAGTCATAAACAGAACTTTCAATCTTGATTTC +CTCGGTCAAATGCCTTTTAAAGACATATTCGACAATCGAAAGGATAATTGGTTGCTTGTAATCAGTTGTC +CACACAACAGAGTTTGAGATGGAACCATCTCTAAATCTTCTCAAAGAGCATTTGGAGCCCCAAAAAGATC +TAAACTGCTCACTATCTTCTGATTCCAAAGGACCCTTGGTGACAAGTTTCTCACATTCGTGGGAGTCAGC +CAACAGACCAATCTGAACATTCACCTTGCTCTGCTGTGAAGGTTTACGCTTGGAAATTTCGAACTCTGTC +ACTAAATTCTTGATTATTACATTGAAGCCCTTTACTCTCTCACCCAAACCATATTTCAACAGTTGTGAAA +TTTTGTTGGTAATGAAGTTCTCATATGTTAGGTGGGTAATCTTTTCGAATGGACCAAATGAATCGTCAGT +AGATGCGTCAATTTGGAGCTGTATGTTGTAATCGTATTTCAAGTGATCAACATTGGCATTCTTCAAAAAC +AACGGATCAAATCTATCCTGAATCACATCATTCAATAGATCCAACGTCTTTTTTGCATGTTGCACTAGCA +GTGCATATGAGTTTCTAGTGACTTTGTGCAAAATATTAATCTTGGATGATTTGTCAAAAATGGTTGGAGT +ATTGAACCCCTCAGAGATATATCTGCTGAGATCTGAAGAGTCGGAGTAAAACTGCAGGTAGCCAGCGGAA +GATAAATCCTGAGTAGCCAAGTATTTGATAACACCTTTAAACAGCTGGTAAGAGGAAAACCCATGCAGCA +AGATTTTGTTTCCCTGAGCACCTCCACCATTGAGGAGAGCAGCAGTCAACGTGGCAAATTCAAAATGACC +AAATCCTCCTTGTGCAACACTCGATCCCAGACCTCTTTGCGATAGCCATAGCCTTCCTAAAATACATGCT +TCTTTGAACTGCTCTGCGGATTTCTTGGCTTTATATAGGTATTTCAAGAAGTAGTCATAAGATGTCATCG +TGAGGATGGAAGAGTTATAAAGTGGGGTTGGAGGTAGTTCCAAGTCTTCCTCTTTCTGGACTCTAATACA +GTTCTTATTTGGTAGCAGCTTTTTAGGGTCAAATACTCTGTATGGGAAGCCCACCAGGACGTTGATGGAG +AATTTAGTCTTGTAGAAGTTGTACTCTGAATCAAGCTGAGAATCTGTAGCCGTCAGTCTCAGTACTGGTG +TAAGAATGTCTCCGTTGAGGTATGTGTAGGATAGTTTCAAAAAGTCGAGACCCTCTTTCTTGAAAATCAC +TCTCAAGTTCTCTGTGAGGTATCCGAGGTAGAATGAACGCTTATGCAAGGCCCTATAGTTCAGATAATCC +TTGGCCTGGAATGATTCAGTAGGCATAGTAACATTGACGTCAATTGCTAGGCCATGTGGAGATTGGATAG +CTGTTTTGAGGCCGAATGACCCCACGATAGAAACATTCTCCGGCTTCTTGTAGTCAAACTTGTAATTCAC +ATTAGTTGGTTTGGGGTCATTAAATGGCACTGTAGTTTTCTTGTTCTTGAACCATGACTCTACTTGATCT +AGTGGCTGGTCCTGAAGTTCAGGTACATCTTGAATCAGTTCATAAAGCTTGTGTAGAAACTTTTCAATCT +TGGCAATCTTGTGGTCTTTCAATTTGACTTCCTTGATCAGTTCATCAATTTGTAGCTTGAAGATATTGGA +TTTGAAGAGCTCTGCGGTCTCTCTCGCTATCTGTATATCTTGAGATGAAAGCTTCGAATGAGGCTTTGAA +GAGGAGGACTTTTGTTGTTCCTCGACATCAACTTCTTCTTCGTCAACTTCGTGGTCATCACCTCCATTAT +TCTCACCATCACTCTCATGCTCGTCGGCAGTCTTCGGGATGTCATCACTAAGCTTTGTCTTGATTAACGA +TGGCTTCTTGGAAGACTTCAGCTCGTCAGATAGCTCTGACTTTCTCTTTAATGCTCCACTCATGATCTAG +GCCAAAACAATGAGTATTTATAGCTCCTTGCACCAAAAAAAGATGATCCCTTAACGAAATAAGGTTTGTC +TAACAATTGGCTGTGTCCAATCAGTGACAATATATACGTTTTCGCTCAGGAAATGGACCGTGTGGATGCA +ATAGTCTAGCGTCCTTCTCTATAACTCACACTGTTAAATTTTTCTTAAATTTTTCTTTGATTCGCGAAAT +TGCTAAATGCGTGAATTTTTTGGACTAGCCTTGAGTTCGCGCGTAATTTTCCTCCTTCGATATTGGTGTG +CAGGTGTTAAGTTCCGAGGGTCAAGATTGCCGAAATTGAGAACGACCGAATTGTCCGTCAACTTTGGCAC +GATAGGAGGTTCTATTTTGCGAACAACTACGACAAATATTCTCTTAGCCAACAACCATTGTGATGTCCAA +GACGATCTCTGAGTCTGATCTCCAGACTGCCCTTCGTAAGGCTACGTCTGCTGATGAGACTGCCCCAAAA +AGAAAGCATGTGAGAGCGTGTATTGTTTACACCTGGGATCACAAGTCGTCACTCTCGTTCTGGAACGCTT +TAAAGATTTTGCCTATTCAGAATGATGAAGTTCAGTTATTTAAGGCTCTAATCACCATCCACAAAGTTCT +TCAAGAAGGTCACCAATCTGCTCTCGCAGAAGGTTACAGGAACTTGAACTGGATCGATGGTTTAGGGAGA +TCTATACAAGGTGATGGATTCAAGGGCTATGGTAGATTGATTAAAGAATATGTCACCTACTTGATCAGAA +AGCTTCAGTTTCATCGGAATCACCGTGGTTTCAATGGAACCTTTGAGTACGAAGAGTATATCTCGTTGGT +GACTGTCCAAAACCCAGATGAAGGTTATGAAGCCATCATGGATTTGATGTCCCTTCAAGACTCACTTGAC +GATCTGCAGAGACTTATTTTCGCAACAATTTCACATGATAAGAGATCTGAATGCAAAATTTCAGCATTGG +TCCCTCTTGTTACTGAATCTTATGGAATCTATAAGTTCATCACCTCGATGTTGAGGGCAATTTACCGTTC +CACAGGATCTGATGATGCTTTAGAGCCATTGAAGCAGCGCTACGAGTCGCAACATGCTCGTTTGTTCGAA +TTTTATGCTGATTGCTCCTCCATTCGCTACTTGACTTCTTTGATCACAATTCCTAGATTACCAGAGACAG +CTCCTAACCTCATGGTTAAGGATGAATCATCCGTCGAAGAGGAGACTCCAAGACCAGAATCTCGTCAGAC +AAATTTGTCAGAACCAATTTCTGAGCAGCCAACTGTCTCCCAGTTCCAGCAACCAATTGCTGCACAACCA +ACTGGTGTCGATTTTTGGAGCAATCAGCAATCTAGTTTTGAGGCTGAGCAAATTCGTTTGCAACAAGAGC +GTGATGCACAAGCTCTCCAACAACAACAGTATGCTGAACAACAAAGAATTTTGTTCGAACAGCAGCAGGT +CCAACAGCAAGAACAGCAGCGTATTGCACAGGAGCAATTGCTTCGCGAGCAGTACCAGTCACAAGCCCAG +GGCCGCGTAGCTGAGTTAGAGCGCGATCTGCTGGCTTTGAGAGGACAATATGAGCAAGACCAACTTCTAC +TACAGCAATATGATCAGCGTGTCAAGGCCTTGGAGACTGAAATTACAGCTACCACTCAATCAGCTACTCA +GCAAATTGGCTCCAAGGATGAGTTGATTCAAAACTTGCATGAGCAAATAACTCTATGGAAGAACAAGTAT +GAGAGTTTAGCTAAGCTCTACTCTCAATTGAGACAGGAACACTTGAACTTGCTAAATAAGTTCAAGAAAC +TCCAACAAAAAGCCGCATCTGCTCAAGAAGCCATTGACAAGCGTGAGAAATTGGAAAAGGACCTGAAATC +GAAGAACGTGGAATTGGCTGGTCTAATCCGTGAACGTGACCGTGCTCGTTTGGACTTGGAGAAAGTTAAG +GGTGGTAAAGGTGATGAGATTGACAAGCTTGAGTTGCAAATTAGAGATCTCACCTTGAAATTAGATGATG +CCGAGAGAAACCAATCATCGAACCTATCCACTATTTTTGCTCAACACAAGAAAGAGATCGAAGCTTTGGA +GAACCAGTTAAGACAGCAATCTTCGGCTTTCAATCCAGAATTGGAACAATTACTAAAGGATAAGGATGAA +GAGTTAGAAATTCTCCAACAAACAATGGACGATGCGCTGGTGGAATTAGCCAAAGCTCAGAAGGAGAATG +AAACAGCTATTGAAGAGCAGATCGACCAAGTTTTGGTTGATCACTTAACGAAGCTTACTTCCTTGGTTGA +TGCTATCTTGCTCAGTGGCCTCAAGAGATTACAAGACGCCTTATATGAATTGGACTCTCCTATGCAAGCT +GGTAACCAGAACTCATCGCCTGAATATTTACTCTCCATTATCGAGAAAGCGTCATCCTCTGCTATGGACT +TCGGTGACTCTTTTAATGACTTCATCATTGATGGGCCAAATGGAGATCACGCTGAGATTATCCGTTCTCT +CAATGACTTCACTTCTTCCATTGCTGACGTTTTATTAAACTCTAAGGGTATTGTGCGTCTTGTTAAATCA +GATGCAGAGGCTGATCAATTCATAGCTACTGCGAGAGCTACTGCAGAGGAATCGGAAGAATTCTTCAAAT +CTTTGCTTTCCGATAGTTTAGAGGGCGAAGTGGAAGAGAAGACTGATGTGGTCATGGCCGGAAATTTGGC +AGTGCAAGAGAAACTTCAAGATCTTACCCAATTGACTGAGGACTTAGCCCCAAGATCTAATCTTTTGAAC +AGCAACGTTGCGAACTTGGGCGACCTTGTGGAAACTGAATTGCAGAATGCGGCCGCCTCCATTTCAAAGG +CATCATCCCAGCTTACTTCGCTACTCTCGAAAGATCAAGACCCATCTATCTCACAAATCGACTTTGAGAT +TAACAAAGCTATTGTTTCCGCTGCGATTGCCGTTACCAATTCCATTGAACTTTTGATTAAAGCAGCTATT +GCTTCACAAGAGGAAATTGTTGCAAAGAACAAAGGCTCAATTTCTAAGACTGAATACTACAAAAAGCACA +ACCGTTGGACAGAAGGCCTTATCTCAGCAGCTAAATCTGTTGCCCTTTCCACTAAGATTTTGATCGAAAC +TGCGGATGGTGTTCTGTCTGGGAATAAATCTCATGAGCAGTTAATTGTCGCATCACATGAGGTGGCTGCT +TCAACTGTTCAGTTGGTAGCTGCTTCGAGAGTCAAAGCTACGTTTTTGTCTAAGACTCAAGACAATTTGG +AAACTGCGTCCAAGAACGTCACATCTGCCTGCAGAAGTCTAGTTAATCAAGTACAAGACATTTTAACTCA +GAGAAGCAACGGCGGTAGTGGTGAGAATGATGTTGATTATTCAAAACTAACAAATCATGAAAACAAAACT +GTGGAGATGGAGCAACAAGTTGAGATTCTGAAATTGGAGAATGCCCTAATCGCAGCAAGAAAAAGATTGG +GAGAAATTAGAAAGTATGGCTACAGAGACGGGGACAGTAGCGAAGAAGAATGAAATAACTTATTACACAT +GTAATAGCTATATTACATGAATATAAAACGATAAATGATCAAATGGTAGGTAACTCTTCCAGTTTCGCAG +GAGTTTAAGTGCGAGCACCCCTTCATTACATTGAAAAGTTACATTAAACACTATAGCTCATGAGAAGCTG +TACTTCTCGAAAGTTCTGAGGAAGTTATAAGAGGGAGGCTCCTTTTTATTTAGTCAATGCAGAGTAAACT +CAACAATGAAGCTCTCTTTTGTTGCTTATGTTGTGGCGTCACTGAAAACATACAGAATACAGTTAGCGAT +AAAACACTCTGGACACTGACTCAAAGATAGTGCACCCTATGATTATCCTTGGACCGCTAAAACACAACTA +TAGCACCCCTTTCTTGATTTGTGGCTGTTTCGTCTACTGTAAAGAAGGAGCTTTGACGTGTTTGCTTTCA +CTTTCCAAATCGGGGATTTTCAGGAGACTGTGTTCTAACAGAATCCTGGACTTCATTATCTTTTAGATAG +AAATTAGGACCCCATTAACCGAAAATATCGATACTACTATTGATAACGACAGAATGACTTAACTGAGTGT +TTCCCATGAACCCCGGCTTTCTACAAAATAGTTTCTTGGTGAACATACAACCGACACTTGCATCCATCAA +ATTCAATAGGCTATGATTCACGAACAGGTAGCAGCTTGTTCTTGGTTGTCAAAACAAAAACAAAACAAGC +TCTCAAACAACTAATCCGAAAACTACCTCAAATATTCCCCAACCTGAATGAATTAAATCAACCGTAGTTG +CGAAAATTTCCATATCATGGGCCTTGCACATATAGTAGCCCCAGGCTTTTCTAAAAAACGCTATGTAGTG +AGTTCTTCAACGCCTATCAAGATAGACTCGTTATGCCGACCGTACAAACAAACGTGCAGAGTGTCACAGA +AATGCAATATTTACTTCACTCCTAGGCACTATATGGAAATCTGAAATAACTTAAACTCAATGAGGCACAG +TAGTGGTTATGACAGGGAGGTATCTGGCGGTTCAAGACTTTTACTAATGCATATTTTATACAGTGTATTC +GATTATGAGAAGAATCTCTTTATATACTTATTATAGGAGGTACCATATAAACTTCATTTCCCTTATGAGG +GTATGGCTTATTTCTTGCTTTCTTATTCATGTAATCAGATTACATTTCCATATTGCGTTTTAGAATTCTT +GAAATCCGGTTAATCCGACGGAATATTTTTCTATTGGCTTGAAATTGAGATGGGTATGCGATTCGTCAAC +AACGTATCAAAATTTAATACTGTTAGTAGAATCACATGTGGCTTAGGTGGAGCTCTTCTAAAGCTCAGTA +TATATAGAGATACTCCCTCAAGGGAAATAATAATAACCTGGGTTATACTCAGTATCCAGGTATGCAGGTG +CTTTCCTAAAAGGGTAATTTACTATTCAGGAAATTATGGTCTACGTGCTTATAAAATTACATGAAGAATA +CATGTATCATAAGCGTTTACATCTAATTCACTTAGAATTAAACCTTATGTTTCGGAAAAAGCGCATCAGA +AACAATCCCATCATTTAATTTCAATAATCGATGCATCTTCAAGGTGCTCGCCGTCAAGCATTGTCTCTGC +GCACTATCAATAATGTCTATGATCACCCAAGGATCGAACCTCCGAACCTCAACATGGATGCCATCTGGAT +ACTGGATACTGGATATTAGATGGCTTTGGGAATGCTACATAGTTAGTTCATAATAAAAGCATCAGCAAAA +AGTCTTAAAGCAGGGTACCGGTTCAGATACCATAAAATGATTACACATAATAAGCTATTCCAAATTTCCA +CAGAAATGCTGTACCATGCATGTCTTTCTGGAAACCAAAAGGACTATTAGCTTAAACAGATGAAGGGGTT +TAAACGTATGCATAAATTATTTCTGATCAATATCAATCTACCTATATATAACACGAACTATCACTTTACA +GACTTTGAGCCTACTTTAGAAATTTTATGATTGCAATGTATACTTCCATTTATTCATATATAAATTCCGT +ATGTTCTGAGTTGGTGAGTTCATTGGCGGGGAGTGCCAAAAAAAGATTTCAAAATCTTGAACTTGAGTGG +TCTTCACGAGGGCATGGATAGCACGCGTCATCATCTACATCTAGATCTTTCCTCTTCTTCGAGATAAGAG +GCTGATATTGACCTTCATGTAGAACAAGGCTGAGCTGTTACTTTCTCGAAAGGGAAACAAAAAATGTAGT +TAGCTACTTTTTGCTCCCCTCAGTTGACTTGAAATATAAGATTTGGTTAGTGGGCAGGATTTTTCTTCAC +TTCAATGCAACCTTTCTTTCAACTCTAATTGGCTTAGGCTATATAGGTCATAAGATTCATGTGATTCTAA +TAAAACTTCACCTATTTTTTTGGTTTCAGACAGGTTGCGCACGGTTGAGTGCTTCCAGTGTGTTCTTTTA +GAGTGCAAATAAGATTGTGATGAGCTTGCAAAATGCTCTAAAGCTCCCTGATTTTGTAGAATCCGAGGTT +ACTCTTTATTCAAATTCTCACTAAGTTAGAAGTGAACCGGGAGACATTCAGATCAACCTTTTCAAATAGC +AGCTAAACAAGAATATCATCTATAGGAGCTTGTCGCTCCTCACGCTCTCCAGTACTTAAAAACTTCTAAA +GTTATGATTCTGTTGAGTTTTCATCTTGCTTCAGATTTGTATAGCTCATAGCCGGCTCAGCAAATTTGGT +CCCAGTATTGACAGCATAGTTGATAAAGAGTTACAGACTGTTCACTTTCCAAATGTCGAAATCTTCAATT +TGTTCTTAGACGAACTGAAAAGCTATCTATTGCATTTAGAGCAAGTGAGCTCTTGAAGATATCCACAAAC +TAATGACCAAAGATATGAGCAAAAATAATCTTTAAATATGTAAATGTCTTATCGTTTTGGCGTATATAGA +GATATCACAAAGGGCAACGTTGAAATGATAAAGCCCTAGCAAATGCAGACATCTGTGCTATTGGTTCAAT +ATTTTTCTGATCAAATTGGATGAGTAGGAGGATATTGAGACATCAACTTTGTTGCTTAAATTTGGGATCT +GTACTTCGCTCTAACATTCGTACCCTAATGTTTGTTGTGTCTTGATACATCTAGCGCACCTTGTTTCTTT +CATTTCTCCTTAGTCGTAGGTCGATCAAAATCGTCTTTGTGTATAATTGTTTGAGCTACTGCGCAAAGCC +TCTGAGATTGGATGATATGTGCTTGGCTAATCAGAACACACAAATAGTGAACAATTGAGTGCACATCTTT +AAGCTTAAATAATTCACTTTTGGAAACAAAAATTTGGATCATTTTGAACCCTTCTGGTGAAAAGGTATGA +GCTAAACAAGCTGCGGTATATATTTTCCCTGCTCCACAAATGGATGGAGAAATGTTCAAAAAAACCATCA +CATTACGAAACTGGAAGATCATTGGGGTTGAAATCACAACATATCGGGCTATACAATTGAGAGTGTCGTG +TCAGCAAAAGAGGCAACTGGCTTCAAACTTTGAGTTGATTTGTGCTTTGATTTTGAAATCATGCGATGCT +GAGATGGCTGTGTAACTTGGATTGTTCCTTTATGTCTACAGTCAATATTTTCATTTAACGCAATTTTGAA +CTGTTTGCCCAGCATTAAAGACAATCTGAAATGAGGCACTGGGATATTTACGATAGTTCCGATTTCGCAA +ATAAACAGACCATTGGGAAATGTGGAGATTACCCCGAAAGATGACACCCACGACTATTATTCTATAGAGA +TAGATAGAAATCAGACATGAAAAGGAAATAAAGCTATTCTAATTTCGTACCTGGATGGATAAAGGCCTAT +TTTGCTTTCTTGGATAATGATAAAGTGTATATGGTGTGTGAGTTGTTGAGAAAATACGTTCTTCTAGTCA +CCCAATCCCTGTGTCTTGCTTCGTGCGTCACAGGGAGCAAACAAACAGGCTTTTTTTTTTTTTCCTGAAT +AAGACTTGAGTGTTTATTTGAGTATAACCGGTAAAGATACTCATGACCCCAAAAAAGTGGAGGATGGAAA +CTAACGGGTCACATGAAATTACAACGATCGCAGAAATGCCAACTCCCATGAGGTAGATTCCTCACAATGA +AAATGATGGCCACGACACAATAGGTATACACGGTGTGTAATTCTGCCTACCATTGAGCAAAGTATTAAAA +GACGCGTCAAAAACAATATAGCGGTTGATGCATCTTGTTTTTTGATAATCTGTAAAGGGATATCTGACCG +ATTCGATTCCGTACAACGTTGGCAAATACTTTTTGAGAGAAAGAAGTACAGCCCAAATGCAATATGCCGC +ATCAGCTCCATTTGACCTACTGCAATTGTCTCACATCTACGCGTGCACCTTATTTGAGGCAACCCACGTA +CTGCAGAATACTGCAAGAGCTGAATATTGATTACCCTTTTCGTCACTGCGCCCTCCAACAGAGAGGTGGC +ATGGGAAACCAGAGGACTGCACCAAAAGTAAAGATCCCCAGAATTCTACTATGGTGATGAGCTATTCGAA +AGTTCCTAGAAGAAGCATGAACAGTTCAAGAATAAAGATATTAAATATTCTTATGGCCCAAATCTCAAGT +AGCCCCAGGAGTTTTCTGGTGAGGAAGCTGGGATCGGGATACTCACGAACAGTTCATAGTCAGGTATCTC +GATGGACGTTCCACCTTAAATATTGCAGATCTTGGCGTCGGTTCCTTTCGCGCGTTTACAGAATCCGACC +AACGCGCTTGGTAAAGAAAATTGTTTATTCTGTAAACTGCCTAGTTTAAGCAGACACTTCTATTAGTGAA +ATTCTACTTGCCAGTTTAAATACAAACATACTTCCAATCACAGATACACAACATCCGCCGAAGATAATGT +CTGTTTCTGACCAGCATATTAGTAAGCAATAGAACCTCTGACCTTCTGACCTATTTCAGAAAAACTTATC +GTTGACCTCTACTTCTGACCGGAAGAACATATTGGAGATACTGATGACACACATAGAAAGAATGCTCTGA +TTGGAAGCAATGGCAAAACGAAGGAGGGAGCATTCTGGGACTTAGATTAGCCGAACTAGCGTGCCTCGTT +GCAGAAAAGGTGGCGCCATAGACTGCGATATGGAAGATGACGCGTCATGATAAAGAGTTCGATTAAGAAT +CGATAAACAGCAATTGGCCGTGATGGTAGTGGTATTGCACAAAACCAGCGCCCAAACCAATGACCTCGAA +GGGTGTCGGATAGATTATAACTTTGCATCAATGGACTTAGGGTATCAATTATGTGTGGCGATGTTTCCTG +TTAGATATATTCTGAGATCGGGTGATAAGTCAGAAGCCTACATTGATAAACCAGCTCTAATTTCGAGGTG +AGTCTATCGGGGTTAAAGTGCCTGATGAAATGAGACTTAAAACTCTCGGTGGTAGATCTAGGTTAGGTCT +AAGCAATTTTAAATAATGTGAAACTGCTGATAAGATTCATCTAAGATCTGTTTCAATTAACTGATTGCTC +AGAAGCAAGCCATTCAAGTTCGCCTTTATAAGACTGAACGGAAAAAGGATAATGTTCATGTTGCACATCT +TCGTTCCACCATTTAGGGCTAAATTAGTGAGTGAAAACGAATCTGGGTTATGGGCACAAATTCCATAGAT +ACGGATGGGCGTCCTTTAACGTTGCTATCAGAACCTCAATCGCCAGGCAGGGAGAGTCCCTTCTTGAGTG +GTTTTAATGCAATTGCCAGTACGACCATGCGATGTGGACCGTCCTTGGATGAGTTGCATGTGCCAAACAC +TACTAAGGATATCATAATCCTTTAACGCATAGCTCAGGACCTTGGTGAGGAAGACATATGTACTCTACTC +TTTCGAAGACTGGGGAGGGATAGATCCTACCACAAAGGTATACGGCAGCTAAAAAAGACTATTGCACTTT +AGGTCAGACCGTTAGGTCCGACACTAAGGCTTATTATTGAGTGTGGACTTCTGGGCGCCTTCTATATTCG +CCAGTAATGTCGAGTCTTTGAACAACACTTACTAGACCAATAATTCGAGAAGAGTAGCAACAGTGGCGGT +TGGAGGACTATCCAGAAGAGTCTCTAAAGTGAACGAACTCCGTTGATTAATTCCAGACACAAGTGGCTAG +GGAATCTGATAAATCCAGCAATAAAATGTCACTCAGCATCAGTAACTGATCTCTTCTTGATCGAAAAATC +ATCCCACCTCGGAAAGTTTGGAGGTGAAAACTCTTCCATGAGAAGAATCACAACGAATCAGATTAAAATC +ACTGTTTATCCAGGCAATGAACCTAGGGGAATTTGACAGATGCCACCAAGATTAATTTTGCTTGCAGATA +AATGGTTTACAGAGTTCCTATAAAGGAGCTGGATTCAAGAATGTTGAATTTGACTGTTGATGACGAGGAG +AGATATAGTTGGCGAAGGCTACGATTACTAATGGTCTTAGAGAAGGGTAATATTTGAAGTACTTGACATG +AAAAAAAATGGAGGAAAATACGTTCAGTATGTGAGATCACTTCTTCAACCACGGGTGGCGTTCACTCACT +CCATTGAAGTATATGGAGACTGCGTAGAGGTCGCTCAGGCAAAATTACAGTGTTGCAAGTTTAGAGAAAG +TGGTGTCACCCCATTAAAATTTTTGCAGGAAGCTGAAATGAAGACTCAAAGCTTATATGTTAAAAGACAT +TCACCACGTCCTATCATGGAGTGGAATATCATGAAAGTGATAACTGAAAAAGGATTGGTTGAGTTGAATT +CTTTCATGAAAGGGACAAAATTTGTAACAACAGAAAACTACAGTCGGGAGAAAGCAAATGAAATAGATCA +AATAGTGATTCTCAAAGTTTGTATCTTTCAAGCTGCCATCCTGAAGGTTTCAAAAGTTCTGATGCAATGT +TAACAATTTCAGAATTTCCTAATAATTTGTGCAAGGAGAAGTGTTCACATATTGGAGTGATAGGCTTAGG +GACAATCGTTTTTAACAAGTCCCGACACTAGACATGAAATTGAATCCCCTATTTTGTCCTCTCGTTCCTT +TCTTCTATCAATGTTGTTAGTACTGTACCGATTCATTACAAACTTTGAAGTGCACACATACCTCTTATTG +GAAATCCTAAGTATAGAAGTTAGTTCATGCTATGATCTCTGACAATCTTACACATACCAATTGCGAATTT +GGCCTTTAGTGAGACCTGTCTTTTTGACCAAAGACAGAAGCATTGATTCATTCAAGTTGGGCTTCTCCGG +ATGACTATAATACCTAAACTATGTTAATTGTAAATTTGAATAGGGTACTGTGCGAGAAAACATACCAATC +CTCAAGAATGGCAACTTTCTGCCTTGCTAATCTTTTCTTTCTAGAAATAATACTTCCATCTTCTCTAATC +ATCTTCTGCGTAATAATATTGAATCTAAAAGGGTCAGGATTGTTAGGAGATTCTGTCGAAGTTGAAAACC +GCTCAAGGGCTTCGACTAAGATGGCCTTCTCGGAGAGCAAAAGCTGAGATTTCAATAAGTCTTTTTGAAT +TTCTTGAAACTGTGACTGATCCTCTGTTGTAGTATTTGAATGTCTTTTCTGAAGTAAATCCAACTCCTTA +GTGTGTTAGTTTGATCGCTTGACATAGAAGTAAAACTTACCATATGTGAGGCTAGGATGTTCGAAGAAAG +TGTGCCAACATCAATGGAGCTAATAAGTGAGCAGCTTTCTAGGATATGTTTCGAGCTCGAGGATAGTAAA +TAGCGTGAACTTATTTAATTGTTAGTACCAAATCTGCATTGCAAGAGTTTCAAAAAGAACATACTCTGCA +GTATCCCCCTGTTGTATATTATCAGTGTCTGAGAGTAATGAAGTTATTGGACATCTGCTAAACTGATCTT +CAGACATTGTTTGGAGGCAGGAAGTGAACTTGTAGTAATAGAATAACAGGGAGCTGGAGAAAGTTTGTTT +CAAGACTGCTCAAAATCCCTTGACAGCATGAACATAAATCACGTGATAGAGATGAAACAACTGATCATGC +AACAGCTAAAGAAACGAATATATATACACATATAATCGCTAGAAAAACTAAGATCTTAGGTGCGTTTTTG +CAATATTTAATAGTAAGAATGTAGGTTATCCCTATTCCTTATAATGTTCAGGTCAACTTCAACTGTAATA +GATGGCGTATTCTTTCACATAACTGATAGCTTCTGTTTCAGATTAAAAATAACAATAACCGCCGTAACTG +TTTGTTTCAAAAAGATAATCAAGGTGGGGAAATAGCTTATCGACTTAGGGAAAGCGAGGAGCCGTTCCTG +CTGTTATAAAGAAGCACTCAAACTTTGGAAGTGACATTAACTGAGTTTCATCAAGGATAGGTGTAGCTTA +AGGTTCTAATAATACTGTTCACACGCTGAGGAGACGATGGCTTTGGTAGAACATTACTAGATGCTAACCC +ATTTGCATCATCCTACATTGACGTTCCTGTATCAACTCTCGAACTAACTGTTTGGTTACAATAGATCAGT +CAATGTATAAAAGTAATACAACAGTAATTCAATTGTTATAAGCTTCGATTCAATTATCGGTTTAGCATAA +ACTATATAGAATGCTCGAATGCTTGTCCTATTCTTGGATCGATCACAGATACATCAAAACTCATCTTTAG +AAACTGGTGGTTCTCATCTGCCTCATTTGTATTGAAAACATCTTGAATTCTAGAGAATGTACCTGGATCC +TTGTTGTCATAAATGTTTTCAGAGATACAAGTAGGCTCTGATGTTACAAGCAAAAGCCAATCGAAAAAGG +GAAGGCGATCACTCAAAACAGACAAATTGTACTCTGTGGAATATCTCTTCCATACAAGTTGGATTTCTGG +ATTCCTCTCCCATTCTTTCGCCAATTGAGAAGATAGGACTCCTTGTTGATCTGTTGCAACATTCCTAGAG +AAGAAGGATCGATAGGCCATAAAACCATTCAACTTTGTCCGCTTTCTTGAGGGCCCTCTCTTTCTCCTCA +AATGTTGGCGGATGTACGTACTACTATCTTGACCAGCTTTATGATCCTTTAGTCCTTCAATAAATCTTTC +ATAATCGGCTGAAAGCTTTGGTATCTTTGGTAGGGACGTAGCGGCAAAAGTAAGACACTGAAAATTCGAA +GGGGAGGTACACTTTGACGGGTTCTTACTTTCAAGGGCATTATTGCGATGTTTCAGTCCAGGAAGAGAGA +AGAGTTTAAATCCAGCACTGTATGGCTTCTTCGGGTTGCTTGTCCACTTTGCTTTCACTTCTTTCCTAGG +CATTGTTTATCAACGTGAACTACTGAATATATGTATATACAGTTGGTTAATTTCATACCACTCTTCAATA +GATCGTGTCCGTCAAGCCGGAAGGTTAATCGTTAAGCCACGCCTCAAAAAGGTCTGCGTTCGCGAAACTG +TGCTTTACAAAAAATTCTTGATGGAAATAATCCTGAAAATATCAATAGAATCTCTAGTTTTCGACTAATA +ACCATACATATTGTTCAATGCTACCCATTTAGTTTGTCCATTCTTCCAACGTCTATTTTACAAGTTCCAT +TTTGACAGATGCTAATAGTAGTAAACTTGCAGTAGCAGCAGCACTTTAACTTCAATCCATTCGGAACATT +TCAAACGTGTTGGTTCGTATTCGTAACGACTATCACATCACGCTCACAAGTATCTCTAAGTATATTAATG +CTCTGCCCCCTATAACAATTTGGGAAGACTAAAATATAGAAAACACAATAATCTCGAGGTGCTCCTGTAG +CTTGCCCTGCCTATGCTTTGCATTGTATCGCATGTTACGAATGTTACGTTTGATGCTAATCATCCAATCT +CTCTTGAAGTCTGACCGTAAAATAGCCGCTTTTTTAATAACCTGATCTGATACATTCGTTTCTTAACATC +ACCGTATATCAATGCAGTCTTCTCCTTATTAAATCATAACTCTACACCTTCTGCTAACTACGCCTGTATC +CTGCCTATCCCCACTCATAGTTTGTTACCGTACAATTAATGATTCTATACTTTATGGTTGTCGCTGGGAA +GGACTCACTTTCTCTGGCGTTCTATTAATCTTGACGAGAAGCTTTGAATAGAAGGACTTTATTAAAATCG +ACATCTTCTTGATGATCTCATATTCGCCACCTTTATTATTCTCGCAGCTTCAATGAAACACTGTAGATCT +TCTTAGCGTTACTTCTTGGGCGTTGGAGAATTTCTGCCTCTTCATAACATCCAATCTTCCTTCGACCTGA +TTTCCAATCATCAAGGGCAAGAATTAAGGCCGGAAACGGGAGCTACAACATTCCACATTTGGCTTCATTC +TTAATATGTGAGTTTGTTTACATCATATTAAGCTCAATGGTTTATTCGGGTTTCACCTGTTTAATGGGAT +TTGTTGACTACCTCTATAAGTTTTCAAATCCAGAATTAGATTCGACGCAGGATTCATCACAGCTTCGATA +ACAGTTTTATAACCTTGCAAAAGCCTGGTTCATCCCCAGCTGAGAATTTACAAGCCATTGAGGATGAGAG +CACTCCCTTAGGTGCTAGTTAATGTGTGTAGTTTTTAGTCTGATTGTCAATTCCACGTGTAATTCTCATC +ACCTCTTTGGGGTCAACTTCCGAGGGCATATATTCCCGGAAGTGAGAATACCTGGACCCAGCATCAGAGC +TAGTGTTTATCTTAACGAGTAGAATGTCAAACTATTTCCTATGCAGCTCTATGACCCAATGGCTGCTCAA +AAAGGATGGCGTCCTGTTCTTTTAACGACACCAAAAATCAAATTCTGGTCGAACGTCCTGTGATTCGAAC +GAGATGTAGAACTTTCTTAAATACGACTGTATGCTGTAAGCCGGGCGGCACAGAAGTTTGTTTGAGTGGC +AACCGGCGAGCAGTAAGGACAGGAGCATCAACTATTAGGTCATGTAGATGTTAATGGGTGCATTAGTAGT +TCCTGTACCGCTCGGGGCGCAGTAAAAACAGAACTTATTCTACAGTATTATTCGCAATCAGCTCCAAATT +CCACAATTACTACATGTTAATCAGCTATACAATACGGCCTTGGTACAGCCGTTTGAACGAAATAGGTGTT +AATTTAAATTTAACTCTGAAGAGAGATCAGAACACCTGGGCTGCGTTCAAACAACCCAAATAAACTGTCA +GGAAGCCGCAAGGAATTTGAGAAGAGTTGAAAAACCTAATAATGTGAAATTGGCTTGTTCTTGAACTTGA +AGCTTGGGCGCGCCTCGATTGTACTCGGAGAAAGTTACAAGTAGTAAACGTAAGAGGATTGGCAAATAGG +AAGGGCGGGAAGGGCAGTTTACAGGCTTAACTTAAAACTATTTGGGGGAGAGGGAAACCGAACAACGTAG +CTGTTTACTATTTCCAGCAAAGGCCATTCAATTCAATAATGGTACACAGATAAAGATAATAAATAGTGTT +CAAAGCTTCTTAAAACAAAAGTTAAAGGAATATTGGAGATGAAGTTCAGAAGTCTCCCAATCGAGATGCT +CATTACGAAGATTAGTGCCCAATGAAAACAGATGGTTAGAGTGTCACATTGTAAGCCACTAGCGAAGATT +ACTAAACTAAGTATACTTAGAGCCATATCAAAAGTCTCTAACAAGAACGAGTAATGGTATCTATGAATGG +ATACTTCGAGATCGAAGTATCTTGTATTTAATAAACATTATATACATAGTACGATAGACGAAAGATGAAG +GTTTGTTGGTATTTGCAAGATAGTTAACCTAGCTCCACTTCGATATCATTGGGATACATATAATGGTTGG +ATTTAAAGACAAGAAAGGTGAGAGTTTAACCTGTCACATCAAACGCCCACTAGTCTCGTCATAACTTCGA +GTGTTTCACCAGCAGGTAAACTATTATCAAGATATGATTCAGAGATAGGCAAAATTTTCCAGATGATGAA +ACCCAAGTCAATAATGTTCTAAGCTATTAAGGTAAACAGAATCGATGATAACAGTGGCAAAGTAGTAAAT +GGTAAGGAGATGCTATTGATGGCTAGTGAGAGTCACAAAAGACATGATGTTTGGCTATGAATTACATTTG +AAACGCGCCCTACAAATCAGAGGCAATAACATGAACTTATATTCTGGTCTTGGTTCTCATACGCTTGTTG +GTGAACCACACTCTTACTTGAAGTGCTGTCATACCGGATTTCTCTGCGATTATTGCTCTTTCCTTTCTGG +TTGGATTTTTAGTAGTTTCAAATGCTTTCTCCAAAAGGCTGCGAGCTTCCTTGGAAATATGTCTTCTTCG +CTTCTTTTGTTGAATATTGTTATTACTGTCTGGTGAGGCCATAACTGCTTCAGAAACAGAACAGACATTA +GAAGTATTCATAGATGATGCTTCCGGAGGAGAATGTGAAGATGGAGATATGTCTGAACAGTTAGATTCTG +TTGCGCTTCTTTCGTTCACAGAAATAGTTGAAGACTTAACCCTGTAAGACTTGCTAAGAAAATATGGTTT +TTCAACACTGTAGTCAATCAGTTCTAGGACCTCAGAACCGTACTTGTCTGCCAATGTGTTAAATAAAAGC +CTTTGATACTGACCTGTGATAAGTTTCTGGTTCATGCCCTTAAGAGACTTGAGAAGTTGTTAGTAAAGCT +TAGTTGGATCAATGGGAGTTTACATACTAAAACTCGAATTAAAGATTCTTCAAAACCATCCAAGAGCTTC +GCCAAATAAGATCTATGGGCCTTAGGAACAGCTTGTAATGTTCTTGATCTATTAAAGGGATCAGTGACAC +TAACTTCATCAACCTCGTTTCTAGAAGGAGGGACAATATTTCCAGCCAATTTTGAAACATCCTTTATCAA +AAGGGCAAGTTGTTGCCTAGCCTGTGCGAGACTGTTTTGCGAAAAGAAGTCATCAAAGTCATCACTTGAG +TACGACATTGATTGAGTAGCGGGCTATGATAAGAGGTTTGTAAATTTAAGGTGTCAAATAAAAGACCCAC +AAGGAGTTTACGATATAGTGATCTAAGTTACAAATTTTGATTTTAGTTAAGGAGTTCTAGTTCGCAAAGA +AAGATAACGTAATCAAGGTTTTAGATTTTTTTCCTACCATTGTTTAACCACACTCGAAACTGTTGCCTCA +AAAACTAACAGACATCCTAGAAGTGAGATTTTTTATGTTTAACTTGTTACTTTAAAATATCTGCTAATAT +ACTCGTCGAAGATAAGTACGGTACTAATAATGGAAAGTCTTAAGATTGCACATTTCAAGGCCAGTGCTGG +CAATGACTCTGATTTGCGAATTAAGAAAAGTATTCTCCCATTTGTTTCTATTCCTAAGGGGCCTTCTAGT +GGCCAGAAGTTTACCTTAACATTCGAAGATTCACCTTCTACTTCGAGCAAGCAATATTCGAATGTTGCTC +GTCCTAGAAACCAGTTCATAGTGGCAAGAATGATTTTGAATAAGATAGTCCTTGCGAACTGCAACGATAC +GATTGCCAATGATGACGTTTCTAAGATAATATCGATAGTATGTAATTGAAACACCTCTCCTGCACGGAGT +CTAAAAGTTACTAACATGCTTAGATTTGGAAAACTTCTTCTTCACCATTTCAAAAATATTTCAAGTACTT +ATCCCTTCTTGAAGAAAGTTGGCACGAAGAGCATTATCCACTTTACCATTATCAACCTAAGGTCAGATCC +AAGACAATTAAAAGCTTAGCACCAGTACTCGAGCAATCGAGAACTATGACTGGGCGGATAAGCAAATGTG +CCCCGTTATCTTCTTTGAAGAAAAAATCTGGGTTTGTCAACACAAGTCCTCACATGATGGGCACCTACGT +TAAATTATCAACTAGCTCTTTTAGACACTCAACTGTTGTTTCAAATGTGAGTATTAATCTTGTTAGTAAG +TTTAGATTGACCAAGGGACACACTTCAGGAACAACTTATAAGAAGCGTGGGCCAAGGTCTTCAAAGGATG +ATGCCTATATAAAAAATAATCTCTGGTGTCTCAGTGGCTTTGACAAAACAAATTATTTTGAACCTTTGTT +TTACGAAGATAAGATTCAGAACATAACGCTAGAAGATTTTAAGGATTACATATGAACAATCTACAATAGC +AAAATATTAAACTTTGATCTGTAAGCCCCCACTAAAAGACAATGGAGAATTTGAAGTTGCATTTATATTT +CAAGCGGTCAGTACTCTCTTGTTTTTTTGTTTACTGCATTATGGATTCATAGGTGATCAGAAACTTTTAC +GGCATTGTGTGCTAAACTCAGACAACTGTAGCTTGTAAGGTTTCTCCGATCTTGATTTCTTCCACCAAAA +TTCAGTTGCAGTATGGCATGTAATATTATGGCAGCCTTTACATTTATCAGTTGAAAAAATGAAGACACTT +TCGACGACAATCGATAAACTATTGACCATCTTTTATGTTAGCCCTTCCCAAATTGGCAATTTCCGCTTGT +TTATATAACTGGAATACTAACCTTTGAAGGCTGGTTCTATGAGCAATGCATAGGACAAAATACGAATTCT +GAACAGCAATCACGCAACTTCTCTAGCTACTGCTTGCACTTGTAGCAGCACAGGCATATCGATCTCAGTA +ATCAGAATTATGTCCACCACTTTGGCTGTCCTGATTATAAAAGCGAAATAATTTACTGCATTTAAAGAAA +ACTTATAAGAGCCGCGAGATCTGTGGTCCTCGAGATCTGTGTGATTCTTGTACTCCATATGAGAACAACC +ACTCAATCTTTAGTCTACCGGTATCCTATTACCTGAGAAGATATCACTTGAGCACCCCGGGGGCGAATGT +TAGTTAGTCACAAAACAAGTCATGTTGGTTCAAATTGGAAAGCATCTGCGTGACTGTCTCTGTATCTTGA +CTCTCAGCTTATCCTCCACATTATGCCACACTCGCTGTTAATTCATCGGCTTTTAACTTCCTTTAACTTT +AATATTGCTCATCTCACATTCAGTAGGCAGATCAAATTCTTCTATTATCACTCCTATGGGGTTATAGGAT +TTCTTATCTAGCTTGGCAATCAGAAGGATCTTGACCTCTTGACATTCTCTAAAAGTATTTTGTTAGTATT +ATAGCGCGGCAGCAAGAGCCATTCATGTTTCTTGCTGGCACTATTCTTGCAAAGTAAAACTTTCTGCCTG +CTGTGTTCTGGTTTGCGATAACGACTCATAAGCAGCTGTGCTAGTTGAAAAGATGTGGACAAATCATGTA +GCTCCGGAATATTTACACGAATATTGATGGGTTATCCAAATTCCTCTATGCAGTTGGTCCTCATGTACTC +CTCTAATTGTTGAAGATTCTTTGCTCTAAATTGCCTATTATTAGCAATGCGTTCCTGGTAATGCCAAACA +TTCGGAAAGAGTTCACAACTTGGAAGGATTTGAACTATAGGGGCCAGCTTAAAACTAGCAGCCGAATCTC +GATTTATTCAAACACGCATGTGGTGGTTCCTCCTTCCACAGCGGTGATGTTCGTCTCATTTTTCAGGGCA +TTAGCAGCTGACAAGAATTCAATTCGTTCTGATCCGCGGCTTTTTAGAAGATATACGCATGGGACTATTA +TGCTATTCACTAGCAAACTCCATTCGGCATGATCCATGTCGCTTCTCTCGTATCTGAGGCATAAAATTAC +TGCCACTCCGATTTGACACTGTTTCACCTCTCGTCTGCCGATTAATACAAGGCGCATGTATACCAACAAA +AGTCCCCATGATCGAGCACTGAACCTTTACTAAATTAAACCAAGTCCTTATTTTGAACATACTCCAGATA +AAACGTCAAATGATAGCATGTTCATCGATCCCTGTTATATGCAATTAATCACCTCAGGTATAGTACGGGT +GCTTTTGACTAGGTCAGGGCAAACTTCAGACAATCCATGGCTTTGGTGGGTACGAAGAATAAGGACGAAT +AGGAAAGGCTTCTTTGCGTATGCAGGATTCAACACTAATATTAGTAATTACTTCCACACTTTGTATCCTG +TAATGAGGGTAACGAGCGTCAAAATCCTGCTGAGAAATACCTACTCGTACGTAGCAATTTAACTAACGTA +CCATATAAGCACAAAAAGTACAAACATGACCCGTGGAGTTGGTTGGCTTTGGCTCAAATTAGTCATTCAG +CTATTTGTTGTGGTATGAATCAATACCAACAGTAGGTGACAGAACGCAGGATCTTTAGTCCCGAAAGGTT +AGGTTTACCAGCTTTCAGGCACATCCTAGCCATGATATGGTTCTGCGCTTCGAGGTCTTCTTGTGTAATA +CAGCGCCGGAAACACTCCATTTCAACATGAGGGTGTTCCACCGCGTTGAGCTAGCACCATTGCCTTCAGA +GTCTAATATTTTGGAATCGGTTGCTTGTTGGAAAGTTTTCTTGTTTCAGTTATTTTTGGATCTAGGAGAG +AGCTAAGGCAAATCGATTGAAACCTATCTCTGTGTAGATGAACTGGTGCTTTTAGAACATGTGAAGATTC +AAGGATGCTACTGGAGGTCTCATTAACTGGTTTTCTTTCTTGGTCGCGGTCATGCCTTGGACTATGATTC +TGTTTCGGGTTTTGCCTCCAAGTTAAATCTCCTATTAACCCTGATAAATTGAATCTTAAATCTCTTGATT +TCGCCCTCTAAGCTATGAGGTACTGCTCCAGCGGTCAAGATTTCCTTATTCTTGAAATAAATTTCACAAC +TCGAATACAAATCTGTGTTTCAAGTGGAGAAATAGGAATTGTAGACGCATACTGTTGTAAATGTTGATAC +AGGTAGAGTTGACGGCGATTCGAGCCATGGTGCACGCAGATCGACGGTGTCGTTAAAACTCGTGGAGCTG +TTGGTGGTAAAGTGTGATTTTTGTGTCAAGTTACATAACCGCTGACAAATCAACACGTCCGATTCCTTCC +TCAGGTTTGACATGCTCTTCGACTTTTGGAAACCAAAATCTAAAAGCCCAGTGGTCAAAAGTGCTATCCA +CAAATCCATGCCGAAAAGGCTTCCTTTTGTATTGAAGCAGCATAGTATCCTCTTTGGTATTATCAGGTCC +CGAACGGAACAGCCAAAATAATCCATGCGGAGTAATTATCCAGAATCATCACATTGCGATGATCTACCGG +TATATCAACCAGCATTTGGTATACGGGCTTCAATTTTACGTTTGCCGTAATAGACATAACATAATTGATA +AGTCAATCTTCGCTGTCTATAAGACTGCTTACTTCGGCTTCTTCAATGAGAGTAGGCTCAGTGATCAGTT +CCCCTTTCAATGGGTATGGAGTGATCGTCAGAGTCTCACGTGTGGGAAACCTACCGCCTCTAAGTCTGCA +TTGCACCTCAGGATAGTGATCTCTTTTGAATTCAAATCTTCTTAAACCCCTCTGCTTCCAAAATCAATCT +GATCCTGTTTGGGAGTTCCAGGTATCAGATATTAGCTTTTGGATGTTTTCACTTGTCATATAGCTATCCA +TTTCACGTAAAATTCTTAGCAAAGCGAACTCTTTGGCTGTCTGTACCCTATTTTGTTTGTGCGACTAGGA +CTGTACGCGTTAGAAACGCGTCTTAAAAAAAAATACTACGACTTTCATTCTAAAGTTACCCGCCTATCCC +ACGAATAAACGCGCCCAGAGGCCACTGATAAAGATCACGTGATAGATTTTAATCAGCAGCAATCTACGAC +CAACGTATATTTATGGGGCAGAGCCAGACAAAATACCTTAAATCTCCCAGAAGGGTGTATGTATGTGATC +GTGGTATTCACAATCATCAGCTAAATATATGCACCTATTACATCTTAATTGCTACCTTGGAACCTATCAG +CTGGCAGGTTGGACGGCTGGTAGTTGTAGCTCGCCACAGCAGCTATATATTAGCTCAAAGATGTTTTCAA +GAGGCACGCACCAAACGTGAGTTGTATTCTCACTGTTTCATATTATAATGCAGTATCCTATTTCATTGTT +AACTTGTTTGTATTGTCTGACAAAAATAAAACTTCTCTTCCCAGCCAACAGCTGAGACAGTAAGAGCAAC +CGCTCCAGAACTGATTAGTTACTTTAATCGAAATTTAGTGGGAGCATTTTGAATCACCAATCTTCAAAGA +GAAAACGTGTACCTTTAAAAAAGCACCTGTCCAATGTACCTCCTTAAACTATTATTTCAAAAAAAGGATA +TGAAAACAAACTGAAACAATATCGATATATTATCACAAAACTTGATAATTCGATATGAGACAATGAAACA +ATGTTTTGTGGAGCGATGCTGATATTAAAAGAGAACAAGTCCCACTAAGTCTCTGCTGTAGTGGTTCATT +GCATTAAAAGGCTATGCTGGCTCATACAAGAATCCAGAGAGCCAAGTGCATTCAATCAATGATGAATTGC +ACTTGCAAATGGATAGCCCGATTCAAATCCACAATCATCAAGCAGACTATGTCCACGTCTAGCCTGACGT +CAACGAGTATAACTCTACAGGCTTTTCTCGATGATATGAAGGCCAGAGGTAAAGATTTGGATAATAGTGC +GCATATTCGAGGTACTTTCTATGAACTTTCTGTGAGAATGACTTTGATGGATGCCCTAAATCTCAAATTG +AAGAACGTTGGTGGGACCAGGGACAATGGTGCTGACCTGATAGGTTTTTGGAATATGTCCGATTTTGCGA +AACCTCAAGCCAATCCAGTCCTTGCCCGAGAATTGGCCCGTGTCATGAGTCCACTGTGCCATGAAAATCA +TGATCGTGTGAAATTTGTGGTTCAGTGCAAGAATTACAAGAGGCTTATTTCCCCTAAGGAAGTCAGAGAG +TTGTCAGGTATATCCAAATTTCAGGGAGCAATTTCTGGCACTCCTGTTGTGACTGTTCTCTGTGCTCCCA +AGCCACTTACACGTGCAGCCCTGCTTCAATTCCGTCTAGCAGGAAAAAGCTTTATTTTCATTCAGATCAA +GCCACATGAATACAAAGGATACGGAGACCGCTATGATTTAAAGAACTACAAGTCTGGCACTATTAAGATC +GGCGGTGAGCATTCGTTTGCTTGTCAGTTTGAGCAAGACAACCTTGCGCTTGTCCGTTTTCTAAAAGAGC +TTTTGACAGCTCTTTCGATTGAGGTGAAGGAAATTCCATTGGACCTGGTTTCAAAGACTAAGCTTTGTAG +TGCAATTCTATTCCATTATCGCTGGGCATGGGTCAGAATTCGTCATCATAGGCATCTGATGGTTAAAGAT +GTTGCCTATCGTGAAACTGTCTTCAAGCTGGAGAACACGAGAATGAGTTTGGAGGATCTTCAAAATAGGT +TGGATGAAATCCCGGAGGGGTTCCTGGAACCTATTGAACAAGTTATCCAAACGCTATTAGAGTCCCTCTC +TCATGTGAACACTGAAAAGACAGAATTTGGAGTCTCCGATATGATCACCGAGATACCTGGTTTGGACCAT +GGCAAGATGAGCCTCTCCTACACTTTAACAATGAAAGCAGATGCAGATAGATGGAGAGAGCGTATCAGAA +GGTCATACAACGATCCTTCGACTCTAAAGAAGTATGTTTTTGGATTAGTTGACAAGATTGAAAGGGTCCC +AGATGGTAAGTTGTCTCATGATACTACCAGTATGGGCGCTTCAAATTTCGCACCTCCCTATGTGACTGAG +AAATCTTCAGACAGTGATTTCTCCTCTATTCCAATTATTGATGCGTTTGGTAACGCTCCTTTTTTGGAAT +GTCCCAAACATTATCGAAGCAGTGTTTAACATACAACTACGCAGGGGATAGAATCCTCTAATGCCTTGGG +TCACCGCGGAGCAATAGTAAAGTTCGAAGGAGTCCAGCGTGAGATAACGAAGCTTGAAAGTAGACATAAA +TCGACCATAAACTACACAATGACGCTTGTTTGGGAGTTTGGCGAGCTTGGAAAGTCCCCACCCGTTAGGT +TTGCAATTCAGGTGGCCTTGAGTAAGTAAATTTTAATTTGGGTCTAAAGTAACTAGGTCAAAACGATTTT +GGTTAGTGGAAATATCGATTTCGTCATTAATACTCGTCTTACATTTGTCTTTATCAAGAGAACCGTTAAG +TGGGGTTCCTTCTTTCCCCTCCCGGATTTGTCCGCAGTTGGACTTTGAAAATGTACTTTTTTTTTTGGCT +TATCTTTTACTTTGAGATTTCCTACCCACTGGTTGGTTTCTGAAAGTCTATTCAAGTTGAACTTACTCGA +ATTCTGAAACTCTGTATACTATAGCTATGTTTCTTAATCTTTGATTCCTACTGATTTGGAGTTATCAAGA +TCTCCCTCCACAAATTCAGCAAAGTCAACTATCCTTGATTCAACAACATAGAGTAGTTTACCTGAACTCT +CCGTGTCGAAGAATATCGATTATCGGGAGTTGAGACGTTTTAAGTTTTCACCAAGGATAAATTCTCCCTC +GTCATGGACTACTCCAATTCCAAAGCGCACATTGTGATATCAGGTCGTCTCCTCGTCACCCTCCTCTTTT +CTAACTATCGAACTTACCCGGACCATCTTTCTTATCCCCACTACAAAACGGTGCGAACCATGATGCCTCA +GATACTCAAATTACGGTTTCAATTTGGCAATTTCAGACCCAGGCATAAAGCTCACTCATACACCTAACGC +ATAAGAAACTATCAACTCACTTAGAGATCCATATCTTTTTTACTTCCTAATTCCCATGCAGGCTAAATAA +ATGCACGTTTTATGTTGAAATTAAGCCTACTATAATGCCTTTGTCAGCCATTACAAAGCTCCAACAATAG +TATTACAAACATGTCTAATATCCCTAATAATATTAGAAGAAGTTAGTGCTAGCAGTATAAATGTTATAAC +TATAGAAGAAGTGCTTTTTAGCCCAAGCTACCTGGAAATTTTGAGCTTATAAGGGTAACTACCCAATGTA +CACACGCACGCATCAAATGTAAACGGATATACCGTCGGCTTTAAAAATCAATCATAGTTCATATGCGACA +CTCTGATTTAATAGAATTTAAAAGTATAGACTTTGCAATTGCCATATAAGCAATTATTAGCATATCCCCA +CGAAATTCATGAAGAGAATCTACAGGGACTGTTCCATATGACCCTTTATTAAAGATCATCATTAAAGAAC +TCTAGTAGAAAAATGACTAAGAGATATCGATGAGGTAGAGTTTGGATAACAACGAACAGTTTTTGAAGAT +TTGAAGCTATTAGTGCAAAGTCGAAGACCAGTAGAATCCTAAAACCTGATTATTCTGAAACCAACCTGTC +TATCGCATTTATAGTTTTATTGTTATAGTTTTAACATAGAAGACACATAGTCTGCAGTGCTTTAAGAGTT +CTCTTTATTGTTTTTACATATACTATTTCGCTTGTCGCTTGGGTTTACTACTATTGGATCTCAAAAATAA +AAAGAAAAAATACCAAAGAGAAAAAAAAAAAAAATTCCTATCTCAATTGAAACCCAACCACACCAGTTCC +AGGAAGTCAACTCGATCGCAACGGGTCAGCCATGTCGTCAGTCACTGCTACTACCCATAGCACCGCCACT +GGGGCTCCAATCGCACTGCAACCTGCAGCATCCGCCAACGCTAAAACCATAACGGCCACCAAGACCCCAA +CCACCAAGTGGCCCTTTTGGTATGGTGGTTTCGGAGGTGCTGTAGCCTGCTTCTGCACCCATCCTCTAGA +TTTGGCTAAGGTGCGTCTACAGACCGCTCCTTTACCCAAGCCATCGCTATTTCAGATGGCGTTCTCGATC +CTGAAAGCAGATGGGATTACAGGTATCTACTCTGGTCTTTCTGCAGGCTTATTGAGACAAGCTACCTACT +CACTGACCAGATTCGGCGCCTATGAGTACTTGAAAGAGAGTTTCGTTCCAGCCGATAAGATGAGTTCAAT +GGCTTATCTATTGCCCATCTCTATGGTTAGTGGAGCTGTTGGTGGGATCGTTGGTAATCCATCTGACATT +GTGAATATCAGAATGCAGAATGACACTGGTTTACCGATCGAACAAAGAAGAAACTACAAGAATGCGTTTG +ATGGTGTGGCCCGAATTGTGACTGAAGAAGGTCCTAGTGCCCTATTTAGAGGCTTGTCTACAAACTTGGT +GAGAGGTGTTCTCATGACTTCATCCCAAGTTGTCACTTACGATTTGGCCAAGGAAATGCTCATCTCCTTC +GGAATGAAAAAGGAGTCCTTCTTCACATTTTTCAGTTCTTCTCTGCTTGCTGGACTGGCCGCAACTACAA +TTTGCTCACCTGCTGATGTGCTCAAAACAAGAATCATGAATGCATCCGGAACAAACCATTCCGTGGTGCA +CATTTTGACTAAAGCTTTGAAGGACGAAGGTCCCAGCTTCATGTTTAGAGGTTGGGTGCCAAGTTTTGTG +AGACTTGGACCAAATACCATCATCATTTTCCTCGTTGTAGAGGAGCTGAAGCGTATGAGAATTGGTTTAT +GATTATTTATAGAGTGTATATCTGTTTGAAAGTTGAATTATCATTGGCTTTATCAGTAAGGTACGGCTGT +ACACATCCGTTATCGTCCCTTTTTTCTTTCTCCTCCCGTTTTTTCCCGTTTGTTTTTCCCGATCTTATCC +GTTTAGGGGGAACTAAACAGCCGTACATCTTTTTTGGGCCTTCTCCACCAAATCTTAGAGCACTGAGGGT +AATAATAAAATTCGCGAAATTATTACCACGATTTATTACCTATTACCGGTAATAGAATGGGAATCAGTAG +TGGTGAATAGGGATTTTTTGGAAAGTGCTAATAATTAGTGGTAATAGAAGGGTAATCAGTAGTACAGGTA +CTAAGGATTTTTAGAGAGTGGCAATAAAATGGCAGTCAGTAATACTCGCAATAGAGGAGAGTCCGGTAAC +AATAATGGTAGTACATTATGGTAGTGTCCTCCCAAAATGAGATAATTCAGGTAGAGTTGCAACGAGTTGA +GTAAAATGCTCAACTCGCGACTGCGATGATTAAGTAGATATCTTGGGCATGTATAGTTGAGCGATCTGGA +AATCGACACTTTCGAAATCTAAGCAAAACTTGTAATCACCGATAATTTTTCCAACACCTCGTTGCTGTTC +TTGTTTTCCAAGCTTCCTCGCTTTAAATGTACCTATCGAAATCTCTAATTGGAACCCATCTGTCAGC + diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.lift.gff b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.lift.gff new file mode 100644 index 00000000..c258abe5 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.lift.gff @@ -0,0 +1,22 @@ +##gff-version 3 +##PAF BC_dic1 316 0 316 + QBLJ01000026.1:100869-152175 51307 49661 50609 948 948 0 AS:i:1604 ms:i:1604 np:i:316 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:316M cs:Z::316 +QBLJ01000026.1:100869-152175 miniprot mRNA 49662 50612 1604 + . ID=L000001;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_dic1 1 316 +QBLJ01000026.1:100869-152175 miniprot CDS 49662 50612 1604 + 0 Parent=L000001;Rank=1;Identity=1.0000;Target=BC_dic1 1 316 +QBLJ01000026.1:100869-152175 miniprot stop_codon 50610 50612 0 + 0 Parent=L000001;Rank=1 +##PAF BC_MATa2 269 0 269 + QBLJ01000026.1:100869-152175 51307 41399 42262 807 807 0 AS:i:1366 ms:i:1395 np:i:269 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:96M56N173M cs:Z::96~gt56ag:173 +QBLJ01000026.1:100869-152175 miniprot mRNA 41400 42265 1395 + . ID=L000002;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATa2 1 269 +QBLJ01000026.1:100869-152175 miniprot CDS 41400 41687 477 + 0 Parent=L000002;Rank=1;Identity=1.0000;Target=BC_MATa2 1 96 +QBLJ01000026.1:100869-152175 miniprot CDS 41744 42265 918 + 0 Parent=L000002;Rank=1;Identity=1.0000;Target=BC_MATa2 97 269 +QBLJ01000026.1:100869-152175 miniprot stop_codon 42263 42265 0 + 0 Parent=L000002;Rank=1 +##PAF BC_MATa1 162 0 162 - QBLJ01000026.1:100869-152175 51307 40289 40775 486 486 0 AS:i:812 ms:i:812 np:i:162 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:162M cs:Z::162 +QBLJ01000026.1:100869-152175 miniprot mRNA 40287 40775 812 - . ID=L000003;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATa1 1 162 +QBLJ01000026.1:100869-152175 miniprot CDS 40287 40775 812 - 0 Parent=L000003;Rank=1;Identity=1.0000;Target=BC_MATa1 1 162 +QBLJ01000026.1:100869-152175 miniprot stop_codon 40287 40289 0 - 0 Parent=L000003;Rank=1 +##PAF BC_MATalpha1 233 0 233 - QBLJ01000026.1:100869-152175 51307 36754 37453 699 699 0 AS:i:1232 ms:i:1232 np:i:233 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:233M cs:Z::233 +QBLJ01000026.1:100869-152175 miniprot mRNA 36752 37453 1232 - . ID=L000004;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATalpha1 1 233 +QBLJ01000026.1:100869-152175 miniprot CDS 36752 37453 1232 - 0 Parent=L000004;Rank=1;Identity=1.0000;Target=BC_MATalpha1 1 233 +QBLJ01000026.1:100869-152175 miniprot stop_codon 36752 36754 0 - 0 Parent=L000004;Rank=1 +##PAF BC_sla2 1046 0 1046 + QBLJ01000026.1:100869-152175 51307 24982 28120 3138 3138 0 AS:i:5179 ms:i:5179 np:i:1046 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:1046M cs:Z::1046 +QBLJ01000026.1:100869-152175 miniprot mRNA 24983 28123 5179 + . ID=L000005;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_sla2 1 1046 +QBLJ01000026.1:100869-152175 miniprot CDS 24983 28123 5179 + 0 Parent=L000005;Rank=1;Identity=1.0000;Target=BC_sla2 1 1046 +QBLJ01000026.1:100869-152175 miniprot stop_codon 28121 28123 0 + 0 Parent=L000005;Rank=1 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.miniprot.gff b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.miniprot.gff new file mode 100644 index 00000000..f813557c --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_QBLJ01000026.1_100869-167498.miniprot.gff @@ -0,0 +1,82 @@ +##gff-version 3 +##PAF MH260578_SLA2 1072 29 1072 + QBLJ01000026.1:100869-152175 51307 25003 28117 1719 3165 0 AS:i:2775 ms:i:2775 np:i:763 fs:i:0 st:i:0 da:i:21 do:i:3 cg:Z:94M2D73M1D99M1D18M7I14M2I256M5I54M4D136M1I29M1I11M1I183M4D59M cs:Z:*tctT:1*ctcI*cagS:2*cttI*cgtK:2*acgC*tctT*gctP*gatE*gagD:2*ccaS:18*ctcK*tcgD:2*aacE*gctT*ttaM:1*attV*ttgV:1*attL*cagK:1*gatT*gaaD*gttT:7*accL:9*caaP:3*gcaV*gaaG:1*tacF*aggK:1*ttgM*aacE:2*gatS*ggtN:1*gggD*agaT*tctL*ataR*caaT*ggtL:1-ggattc*aagR*ggcR*tatF*ggtQ*agaV:2*aaaP:3*accN:1*ttgI*atcL*agaQ:2*cagR:2*cggH:14*atcV:2*gtgR:2*caaS*aacD:1*gatN:3*gaaD:2*atgL:2*atgT:7*gatS*ctgF*cagG:1*cttD:4*attV-tca*catR*gatN*aagN*agaN:3*aaaI:6*cttI:1*actA:8*atcL*accI:5*attM:1*cgtK*tccM*acaS*ggaD:1*gatA*gatE*gctI:5*cagS*cgcK*tacF:1*tcgD:2*gctE:2*ttcY*gaaD:3*gatN:2*tccN:5*tctT:5*agaK:2*gagF*acaE:4*atgI*gttI*aagQ*gatN*gaaD*tcaE*tccD*gtcE*gaaN*gagV*gagN*actL:1-aga:1*gaaL*tctT*cgtE:1*acaK*aatT*ttgA*tcaA*gaaT*ccaT*attL*tctR*gagH*cagS:2*gtcP+FEQENER*tccQ:1*ttcQ:1*caaA:3*gcaT:4*gtcM+VR*gatN*tttA*tggF*agcV*aatE:2*tctQ*agtL*tttY:1*gctQ*gagQ:1*attQ*cgtQ:1*caaE:1*gagQ:1*gatQ:1*caaE*gctL*ctcQ:4*tatQ*gctQ*gaaL*caaA:1*agaQ*attQ*ttgY*ttcW:4*gtcR:2*caaA:1*cagA:1*cgtQ*attL:2*gagQ:3*cgcL*gagD:1*tacA:1*tcaR*caaH:11*ctgI:1*gctT:1*agaK:3*gagD*caaN:3*ctaM:2*caaS:3*cgtK:1*aagQ:3*actN:1*attL*acaA*gctS:3*tcaT:1*actQ*cagE:1*attL*ggcA:4*ttgQ*attL*caaN*aacL:1*catQ:2*ataV*actE*ctaY:16*cagT:6*aatT:1*ttcS:2*ctcI:11*gacE:6*aagR:1*ctgM:1*tcgA:2*gtgI:3*ggtD:2*cgtK:7*gacE:2*aaaR*gttF:1*ggtN:1*aaaT*ggtK*gatT*gagD*attL*gacQ*aagT:1*gagQ:1*caaE*attK*agaD*gatE:1*accE*ttgQ:2*gatA*gatT*gccL*gagD:1*aacA:2*tcgA:2*tccT*actA:2*gctN:2*aagN:2*atcL*gaaQ*gctE:1*gagQ:1*cagK+FKDVT:1*agaN*cagA*caaP:1*tcgD*gctE*ttcK*aatL*ccaK*gaaQ:2*caaE*ttaQ:1*aagQ*gatE:2*gaaL:1*ttaI:1*attM*ctcM:5*gatE*gcgT*ctgI*gtgK*gaaD:1*gccV:1*gctT:1*aagN*gagS*aatR:1*acaE*gctK*attS:1*gagM:1*atcN*gacK*caaL-gttttggttgat*cacN*ttaS:2*cttV*actL*tccG:1*gttI:4*ctcK:2*ctcI*aagN:1*ttaI:3*ttaV*tatF:10*cagL:6*ttaV:1*tccT*attL*atcL:2*gcgS:1*tccG*tctL*gctV*atgT:2*ggtA*gacS:3*gacN*ttcM*atcL*attV:2*ccaD:1*ggaA*gatN*cacE:1*gagL*attV:1*cgtD:1*ctcI*aatT*gacN:2*tctT:2*gctS:1*gttI:3*tctT:2*attL*gtgT:2*gttT:1*tcaQ:1*gcaD*gagF*gctQ:1*caaD*ttcL:1*gctD:2*agaK*gctD*actI:2*gaaI:2*gaaV*ttcY*ttcL*aaaE:2*cttY*tccH*gatS*agtN:1*gagV*ggcN*gaaK+S*gtgI*gaaD*gagD*aagQ:2*gtgI:1*atgI*gccN:2*ttgI*gcaD:3*aaaI:2*gatT:1*accL:2*actV:1*gacS*ttaM+K*gccT:1*agaS:1*aatK*cttI*ttgD*aacL:1*aacK:1+D*gcgG*aacE:1*ggcS:3*gaaD*actK:1*ttgM*cagE*aatH:1*gccS*gccR*tccV:1*tcaE*aagE:2*tccV*cagH:1*actS:3*tcgN*aaaQ*gatP*caaF:1*ccaV:1*atcM:1*caaS:2*tttI:1*attV:2*gctS:1*gttL*tccA:2*attS:1*gttI*accI*aatT*tccA:1*gaaK*cttY:2*aaaR:1*gctS:1*gctE:6*gcaN*aagN*aacG*aaaR:2*attH:3*gaaS*tacF:3*cacN:1*cgtK:8*gctS:2*gttI:1*cttY:2*aagN*attT:2*gaaR*actI:5*tctQ*gggN*aatK*aaaH:1*catN:1*cagE:1*attV:3*catR:6*gttA:3*gctS:1*tcgA:3*gctS*acgQ:1*ttgM:4*gacN*aatK:2*actD:3*aacK:1*acaN*tctL:2*agaK*agtQ:2*aatD:2*caaN*gacK*attL*ttaI*actI-cagagaagcaac:1*ggtK*agtH*ggtE*gagV*aatE:1*gttI:5*acaS*aatI:5*gtgA:15*atcS*gcaS:10*tatF*ggcS:1*agaK:1*gggD:2*agcD:2 +QBLJ01000026.1:100869-152175 miniprot mRNA 25004 28117 2775 + . ID=BC000001;Rank=1;Identity=0.5431;Positive=0.7232;Target=MH260578_SLA2 30 1072 +QBLJ01000026.1:100869-152175 miniprot CDS 25004 28117 2775 + 0 Parent=BC000001;Rank=1;Identity=0.5431;Target=MH260578_SLA2 30 1072 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 195 44 154 - QBLJ01000026.1:100869-152175 51307 36943 37279 120 339 0 AS:i:161 ms:i:161 np:i:58 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaT*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaK:2*agtL*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS*agaS*aagR*agaH*gggR*cccV*tcaQ*agaK*aagE*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatR:2 +QBLJ01000026.1:100869-152175 miniprot mRNA 36944 37279 161 - . ID=BC000002;Rank=1;Identity=0.3540;Positive=0.5133;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 45 154 +QBLJ01000026.1:100869-152175 miniprot CDS 36944 37279 161 - 0 Parent=BC000002;Rank=1;Identity=0.3540;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 45 154 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATa1 181 122 181 - QBLJ01000026.1:100869-152175 51307 40289 40466 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +QBLJ01000026.1:100869-152175 miniprot mRNA 40287 40466 171 - . ID=BC000003;Rank=1;Identity=0.5593;Positive=0.7458;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +QBLJ01000026.1:100869-152175 miniprot CDS 40287 40466 171 - 0 Parent=BC000003;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +QBLJ01000026.1:100869-152175 miniprot stop_codon 40287 40289 0 - 0 Parent=BC000003;Rank=1 +##PAF 5476_sc5314_MTL_A_MTLA1 210 116 197 - QBLJ01000026.1:100869-152175 51307 40298 40517 105 243 0 AS:i:169 ms:i:158 np:i:49 fs:i:0 st:i:0 da:i:24 do:i:9 cg:Z:14M7I1M1I58M cs:Z::2*gaaD*gcaQ*gttM*atgD*gccD*tcaD*ccaK:2*aatE*aacD*aatD+ILIKGEE:1+K*caaK*caaS:2*cgaK:1*agaQ*catR*attL*tccD*aagN*gaaS*gctT*cgcK*agcE*cttF:3*gcaV:2*actK*actN:1*aatQ:1*accN:1*aagR:2*gcaE*ataL:4*tccH:1*atgV*acaS*gcaL*cttS:1*gtaI:10*accK:1 +QBLJ01000026.1:100869-152175 miniprot mRNA 40299 40517 158 - . ID=BC000004;Rank=1;Identity=0.4321;Positive=0.6049;Target=5476_sc5314_MTL_A_MTLA1 117 197 +QBLJ01000026.1:100869-152175 miniprot CDS 40299 40517 158 - 0 Parent=BC000004;Rank=1;Identity=0.4321;Target=5476_sc5314_MTL_A_MTLA1 117 197 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1019 7 1019 + QBLJ01000026.1:100869-152175 51307 24997 28108 1533 3135 0 AS:i:2456 ms:i:2448 np:i:714 fs:i:0 st:i:0 da:i:15 do:i:12 cg:Z:259M1I6M3I15M7D7M4D5M2I254M6D35M4D11M7D70M1I34M1D25M1I227M4D56M cs:Z::1*gagN*tctL:1*ctcI:1*actA*gccS:1*cgtK:2*acgC*tctT:2*gagA*actA:20*ctcR*tcgE:2*aacH*gctC:2*attL:4*aatS*gatN*gaaD*gttT:1*ttaI:2*gctT:2*accV:9*caaP*tctT*gctC:1*gcaI*gaaG:2*aggK:1*ttgI*aacS:1*atcL*gatE*ggtS:1*gggS:1*tctF*ataS*caaN*ggtN*gatG*ggaT*ttcA*aagA:2*ggtT:7*accF:2*atcE*agaQ:2*cagK:2*cggH*aatD:6*accM:6*atcV:2*gtgR:2*caaS*aacD:1*gatN:2*tatF:1*gccS:4*atgL:7*gatN:3*cttV:2*gcaS*acaS:1*tcaR:1*gatT*aagS*agaE:3*aaaV:2*gcaS:3*cttI*gttI*actA:8*atcL*accI:3*aggK:1*attL:1*cgtK:1*acaS*ggaE:2*gatE*gctV*ttaI*gagA:3*cagD:1*tacF*gagD*tcgV:2*gctR:12*cgcK:3*tctT:1*atcV:6*gagS*acaS*gctP:1*aacI:1*atgQ+D*gttE*aagE:1*gaaP*tcaQ*tccG+FPL:1*gaaR:1*gagR*actS*ccaS*agaV*ccaS*gaaN:1*cgtE*cagE*acaS:1*ttgI-tcagaaccaatttctgagcag:1*actR*gtcS:1*cagN*ttcI:1-caaccaattgct*gcaS:4+AM:2*tttS*tggF*agcV*aatR:2*tctQ*agtE*tttL:1*gctE*gagQ:1*attR*cgtQ*ttgA*caaE*caaE*gagR:1*gatQ:1*caaE*gctL:1*caaE:2*cagM*tatL*gctQ*gaaR*caaD:1*agaA*attR*ttgF*ttcW:1*cagE:2*gtcR:2*caaA*gaaV*cagS:1*cgtE*attL:2*gagS:3*cgcA*gagD:1*tacM:1*tcaR:4*cgcK*gtaL:1*gagQ:2*cgcQ:3*gctT:1*agaG:2*tatH:1*caaR:3*ctaM:1*cagE:7*gccV:2*actN:1*attL*acaR*gctI*accV:2*tcaT:1*actE*cagE:1*attL*ggcA*tccV:1*gatT*gagS*ttgQ*attC*caaS*aacH:1*catE:2*ataV*actQ*ctaY:23*aatG:3*aaaI*ctcV:9*gccS:12*tcgA:2*gtgI:3*ggtD:2*cgtK:9*gagA*aaaR*gttI:2*ggtN*aaaD*ggtS*gatE*gagQ*attV*gacQ*aagM:1*gagR*ttgT:1*attL:2*ctcM*accE*ttgT:2*gatK*gatS*gccL:1*agaE*aacT*caaH:1*tcgQ:2*tccA*actS*attL:1*gctS:2*aagS:4*gctE*ttgI-gagaaccagttaagacag*caaK*tctA:2*ttcS*aatE*ccaL*gaaK*ttgE*gaaG*caaG:1*ctaN*aagE*gatE*aagA*gatQ*gaaQ:1*ttaV*gaaQ*attM:3*acaA:3*gcgT*ctgM*gtgQ:3-aaagctcagaag:1*aatQ*gaaH*acaQ:3*gagK*cagE*atcA*gacE-caagttttggttgatcactta:1*aagR*cttV*actL*tccR:1*gttI:1*gctT*atcL:1*ctcK*agtV*ggcS*ctcS*aagD:2*caaR*gacE*gccF*ttaV*tatF*gaaN:1*gacE:2*atgV*caaY*gctS*ggtN*aacT*cagD*aacI*tcaP*tcgT*cctA:3*ctcG*tccT*attM*atcL:1*aaaR*gcgV:1*tccH*tctL*gctS*atgT*gacQ:1*ggtS*gacT*tctT*tttM*aatT:1*ttcY:1*attA:1*gggN*ccaM*aatK:1*gatE+S*cacE*gctS*gagG*attL:1*cgtK*tctA*ctcI*aatI*gacE*ttcL:1*tctD:2*gctG*gacN*gttL*ttaF*ttaS:1*tctV:1*ggtA*attV*gtgL:1*cttD*gttR:1*tcaD:1*gcaV*gagQ-gct*gatE*caaI*ttcL*ataV*gctE*actG*gcgS*agaK*gctD*actL:1*gagH*gaaM*tcgV:1*gaaL:2*aaaE:1*ttgM:2*gatN*agtH+L:1*gagK*ggcL:1*gtgI*gaaH:1*aagR:2*gtgR:1*atgI*gccT:2*ttgI*gcaN:3*aaaV*cttI:1*gatS:1*accM:1*ttgV*actV:1*gacV*ttaF*gccR*ccaD*agaP*tctG*aatR*cttK*ttgT*aacP*agcV*aacS*gttS*gcgD*aacE:2*gacY*cttF*gtgL*gaaD*actK:2*cagA:1*gcgT*gccQ:1*tccA*attV*tcaE:2*tcaA*tccG*cagL:1*actE:1*ctaM*ctcS*tcgT*aaaR*gatH*caaK*gacL*ccaE*tctV*atcQ*tcaE*caaD*atcG*gacS*tttL:1*attV:1*aaaE:2*gttI:1*gctC:1*attS:1*gttI*accI:1*tccA*attV*gaaK:2*attL*aaaE:1*gctC:1*gctD*tcaA:5*gcaS*aagR*aacG*aaaR:2*attM:1*aagR*actA*gaaL*tacF:3*cacN:10*gctS:1*tctQ:2*cttY*tccA:1*aagG:3*gaaR*actM:5*tctA:1*aatT*aaaN:1*catS:1*cagE:5*catN:3*gctS*tcaA:1*gttA:3*gctS*gctS:4*gctS*acgD*tttL*ttgM:1*aagQ*actS*caaH*gacL:3*actE:2*aagR*aacK:3*gccS:1*agaK*agtM:2*aatT*caaK:1*caaR*gacS*attL:1*actL-cagagaagcaac*ggcS*ggtD*agtE*ggtS*gagN*aatK*gatT:4*aaaQ:2*aatA:3*aaaR:1*gtgA:1*atgL:10*aatS:2*atcS*gcaH:10*tatF*ggcS:3*gggE*gacE:1 +QBLJ01000026.1:100869-152175 miniprot mRNA 24998 28108 2448 + . ID=BC000005;Rank=1;Identity=0.4890;Positive=0.6833;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +QBLJ01000026.1:100869-152175 miniprot CDS 24998 28108 2448 + 0 Parent=BC000005;Rank=1;Identity=0.4890;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 187 128 187 - QBLJ01000026.1:100869-152175 51307 40289 40466 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +QBLJ01000026.1:100869-152175 miniprot mRNA 40287 40466 171 - . ID=BC000006;Rank=1;Identity=0.5593;Positive=0.7458;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +QBLJ01000026.1:100869-152175 miniprot CDS 40287 40466 171 - 0 Parent=BC000006;Rank=1;Identity=0.5593;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +QBLJ01000026.1:100869-152175 miniprot stop_codon 40287 40289 0 - 0 Parent=BC000006;Rank=1 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 190 41 151 - QBLJ01000026.1:100869-152175 51307 36943 37279 126 339 0 AS:i:172 ms:i:172 np:i:59 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaS*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaN:3*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS:1*aagR*agaH*gggR*cccV*tcaQ*agaK*aagQ*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatI:2 +QBLJ01000026.1:100869-152175 miniprot mRNA 36944 37279 172 - . ID=BC000007;Rank=1;Identity=0.3717;Positive=0.5221;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 42 151 +QBLJ01000026.1:100869-152175 miniprot CDS 36944 37279 172 - 0 Parent=BC000007;Rank=1;Identity=0.3717;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 42 151 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 294 0 294 + QBLJ01000026.1:100869-152175 51307 49733 50609 504 882 0 AS:i:856 ms:i:856 np:i:214 fs:i:0 st:i:0 da:i:72 do:i:0 cg:Z:98M1I99M1I95M cs:Z:*gccM:1*gctS*aaaD*accK*ataS:1*gccK*accS*aagI*accA:1*accS*accA*aagN*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctK*ttaV:1*aagG*ccaD:2*tttV*cagS*atgL:2*tcgK:1*ctgI:1*gcaT*gatE:1*attV*acaL*ggtA*atcA:1*tctA:2*tctT:1*ggcS:8*ctgT*accA:3*gccV:2*tacK:3*agtI*ttcM*gttT+D:1*gccT*gatR*aagG*atgQ*agtA:1*atgT*gctF*tatQ:2*cccA*atcA:2*gttI*agtA:5*atcV:4*tctA:1*attV:8*actN*ggtS:2*atcE*gaaS:3*aacH:2*aatH:1*tttL:2*gtgL*gccL*cgaK:1*gtgT*actR:2*ggtN*cctL*agtT:6*tctG*acaP:2*gtgA:2*gttI:3*tcaA:4*actS:2*ttgV:2*gaaK*atgL:1*atcV+E*tccN*ttcL*ggaS:1*aaaD*aagP*gagK*tccT*ttcK*ttcA:1*tttH:2*tctA:2*cttI:3*gccV:3*attV:13*gcaS:3*aacG*catQ:1*gtgS*gtgF*cacG:2*actK*aaaD:1*ttgI*aagS*gacR:2*cccL*agcG:6*gtgT:1*agtA:1*gtgI:4*aatH:2*atcL*attT:1*ctcI*gttA*gtaL:3*aagR:1*atgL*agaK:3 +QBLJ01000026.1:100869-152175 miniprot mRNA 49734 50612 856 + . ID=BC000008;Rank=1;Identity=0.5714;Positive=0.7279;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +QBLJ01000026.1:100869-152175 miniprot CDS 49734 50612 856 + 0 Parent=BC000008;Rank=1;Identity=0.5714;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +QBLJ01000026.1:100869-152175 miniprot stop_codon 50610 50612 0 + 0 Parent=BC000008;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 305 7 305 + QBLJ01000026.1:100869-152175 51307 49730 50609 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gacK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +QBLJ01000026.1:100869-152175 miniprot mRNA 49731 50612 818 + . ID=BC000009;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 8 305 +QBLJ01000026.1:100869-152175 miniprot CDS 49731 50612 818 + 0 Parent=BC000009;Rank=1;Identity=0.5537;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 8 305 +QBLJ01000026.1:100869-152175 miniprot stop_codon 50610 50612 0 + 0 Parent=BC000009;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1045 0 1045 + QBLJ01000026.1:100869-152175 51307 24988 28108 1827 3165 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D54M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gagS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE*agtE +QBLJ01000026.1:100869-152175 miniprot mRNA 24989 28108 2946 + . ID=BC000010;Rank=1;Identity=0.5773;Positive=0.7488;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +QBLJ01000026.1:100869-152175 miniprot CDS 24989 28108 2946 + 0 Parent=BC000010;Rank=1;Identity=0.5773;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1044 0 1044 + QBLJ01000026.1:100869-152175 51307 24988 28105 1827 3162 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:15 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D53M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gagS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE +QBLJ01000026.1:100869-152175 miniprot mRNA 24989 28105 2946 + . ID=BC000011;Rank=1;Identity=0.5778;Positive=0.7495;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +QBLJ01000026.1:100869-152175 miniprot CDS 24989 28105 2946 + 0 Parent=BC000011;Rank=1;Identity=0.5778;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 305 7 305 + QBLJ01000026.1:100869-152175 51307 49730 50609 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gacK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +QBLJ01000026.1:100869-152175 miniprot mRNA 49731 50612 818 + . ID=BC000012;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 8 305 +QBLJ01000026.1:100869-152175 miniprot CDS 49731 50612 818 + 0 Parent=BC000012;Rank=1;Identity=0.5537;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 8 305 +QBLJ01000026.1:100869-152175 miniprot stop_codon 50610 50612 0 + 0 Parent=BC000012;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 305 7 305 + QBLJ01000026.1:100869-152175 51307 49730 50609 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gacK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +QBLJ01000026.1:100869-152175 miniprot mRNA 49731 50612 818 + . ID=BC000013;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 8 305 +QBLJ01000026.1:100869-152175 miniprot CDS 49731 50612 818 + 0 Parent=BC000013;Rank=1;Identity=0.5537;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 8 305 +QBLJ01000026.1:100869-152175 miniprot stop_codon 50610 50612 0 + 0 Parent=BC000013;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1044 0 1044 + QBLJ01000026.1:100869-152175 51307 24988 28105 1827 3162 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:15 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D53M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gagS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE +QBLJ01000026.1:100869-152175 miniprot mRNA 24989 28105 2946 + . ID=BC000014;Rank=1;Identity=0.5778;Positive=0.7495;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +QBLJ01000026.1:100869-152175 miniprot CDS 24989 28105 2946 + 0 Parent=BC000014;Rank=1;Identity=0.5778;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 287 14 287 + QBLJ01000026.1:100869-152175 51307 49796 50609 477 819 0 AS:i:799 ms:i:799 np:i:201 fs:i:0 st:i:0 da:i:135 do:i:0 cg:Z:77M1I99M1I95M cs:Z::1*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctK*ttaV:1*aagG*ccaD:2*tttV*cagS*atgL:1*ttcY*tcgK:1*ctgV:1*gcaT*gatE:1*attV*acaL*ggtA*atcA:1*tctA:4*ggcS:8*ctgT*accA:3*gccV:2*tacK:2*gagG*agtI*ttcM*gttT+D:1*gccT*gatK*aagG*atgQ*agtA:1*atgT*gctF*tatQ:2*cccA*atcA:2*gttI*agtA:5*atcV:4*tctA:1*attV:8*actN*ggtS:2*atcE*gaaS:3*aacH:2*aatH:1*tttL:2*gtgL*gccL*cgaK:1*gtgT*actR:2*ggtN*cctI*agtT:6*tctG*acaP:2*gtgA:2*gttI:3*tcaA:4*actS:2*ttgV:2*gaaK*atgL:1*atcV+E*tccN*ttcL*ggaS:1*aaaD*aagP*gagK*tccT*ttcK*ttcA:1*tttH:2*tctA:2*cttI:3*gccV:3*attV:13*gcaS:3*aacG*catQ:1*gtgS*gtgF*cacG:2*actK*aaaD:1*ttgI*aagS*gacR:2*cccL*agcG:6*gtgT:1*agtA:1*gtgI:4*aatH:2*atcL*attT:1*ctcI*gttA*gtaL:3*aagR:1*atgL*agaK:3 +QBLJ01000026.1:100869-152175 miniprot mRNA 49797 50612 799 + . ID=BC000015;Rank=1;Identity=0.5824;Positive=0.7363;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +QBLJ01000026.1:100869-152175 miniprot CDS 49797 50612 799 + 0 Parent=BC000015;Rank=1;Identity=0.5824;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +QBLJ01000026.1:100869-152175 miniprot stop_codon 50610 50612 0 + 0 Parent=BC000015;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 195 44 154 - QBLJ01000026.1:100869-152175 51307 36943 37279 120 339 0 AS:i:161 ms:i:161 np:i:58 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaT*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaK:2*agtL*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS*agaS*aagR*agaH*gggR*cccV*tcaQ*agaK*aagE*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatR:2 +QBLJ01000026.1:100869-152175 miniprot mRNA 36944 37279 161 - . ID=BC000016;Rank=1;Identity=0.3540;Positive=0.5133;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 45 154 +QBLJ01000026.1:100869-152175 miniprot CDS 36944 37279 161 - 0 Parent=BC000016;Rank=1;Identity=0.3540;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 45 154 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 181 122 181 - QBLJ01000026.1:100869-152175 51307 40289 40466 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +QBLJ01000026.1:100869-152175 miniprot mRNA 40287 40466 171 - . ID=BC000017;Rank=1;Identity=0.5593;Positive=0.7458;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +QBLJ01000026.1:100869-152175 miniprot CDS 40287 40466 171 - 0 Parent=BC000017;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +QBLJ01000026.1:100869-152175 miniprot stop_codon 40287 40289 0 - 0 Parent=BC000017;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 440 0 440 + QBLJ01000026.1:100869-152175 51307 26746 28108 591 1371 0 AS:i:863 ms:i:863 np:i:295 fs:i:0 st:i:0 da:i:975 do:i:12 cg:Z:15M4D11M7D70M1I34M1D25M1I224M1I7M5D51M cs:Z:*gaaQ*attM:3*acaA:3*gcgT*ctgM*gtgQ:3-aaagctcagaag:1*aatQ*gaaH*acaQ:3*gagK*cagE*atcA*gacE-caagttttggttgatcactta:1*aagR*cttV*actL*tccR:1*gttI:1*gctT*atcL:1*ctcK*agtV*ggcS*ctcT*aagD:2*caaR*gacE*gccF*ttaV*tatF*gaaN:1*gacE:2*atgV*caaY*gctS*ggtN*aacT*cagD*aacI*tcaP*tcgT*cctA:3*ctcG*tccT*attM*atcL:2*gcgV:1*tccH*tctL*gctS*atgT*gacQ:1*ggtS*gacT*tctT*tttM*aatT:1*ttcY:1*attA:1*gggN*ccaM*aatR:2+S*cacE*gctS*gagG*attL:1*cgtK*tctA*ctcI*aatT*gacE:2*tctD:2*gctG*gacN*gttL*ttaF*ttaS:1*tctV:1*ggtA*attV*gtgL:1*cttD*gttR:1*tcaD:1*gcaV*gagQ-gct*gatE*caaI*ttcL*ataV*gctE*actG:1*agaK*gctD*actL:1*gagH*gaaM*tcgV:1*gaaL:2*aaaE:1*ttgM:3*agtH+L:1*gagK*ggcL:1*gtgI*gaaH:1*aagR:2*gtgR:1*atgI*gccT:2*ttgI*gcaN:3*aaaV*cttI:1*gatS:1*accM:1*ttgV*actV:1*gacV*ttaF*gccR*ccaD*agaP*tctG*aatR*cttK*ttgS*aacP*agcV*aacS*gttS*gcgD*aacE:2*gacY*cttF*gtgL*gaaD*actK:2*cagA:1*gcgT*gccQ:1*tccA*attV*tcaE:2*tcaA*tccD*cagL:1*actE:1*ctaM*ctcS*tcgT*aaaR*gatH*caaK*gacL*ccaE*tctV*atcQ*tcaE*caaG*atcG*gacS*tttL:1*attV:1*aaaE:2*gttI:1*gctC:1*attS:1*gttI*accI:1*tccA*attV*gaaK:2*attL*aaaE:1*gctC:1*gctE*tcaA:5*gcaS*aagR*aacG:3*attL:1*aagR*actA*gaaL*tacF:3*cacN:10*gctS:1*tctQ:2*cttY*tccA:1*aagG:3*gaaR*actM:5*tctA:1*aatT*aaaN:1*catS:1*cagE:5*catN:3*gctS*tcaA:1*gttA:3*gctS*gctS:4*gctS*acgD*tttL*ttgM:1*aagQ*actS*caaH*gacL:3*actE:2*aagR*aacK:3*gccS:1*agaK*agtM:2*aatT*caaK:1*caaR*gacS+L*attL:2*cagD*agaE:2-ggcggtagtggtgag*aatK*gatT:4*aaaQ:2*aatA:3*aaaR:1*gtgA:1*atgL:10*aatS:2*atcS*gcaQ:10*tatF*ggcS:3*gggE*gacE:1 +QBLJ01000026.1:100869-152175 miniprot mRNA 26747 28108 863 + . ID=BC000018;Rank=1;Identity=0.4311;Positive=0.6455;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +QBLJ01000026.1:100869-152175 miniprot CDS 26747 28108 863 + 0 Parent=BC000018;Rank=1;Identity=0.4311;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 671 0 671 + QBLJ01000026.1:100869-152175 51307 24988 28117 825 2121 0 AS:i:1065 ms:i:1065 np:i:398 fs:i:0 st:i:0 da:i:6 do:i:3 cg:Z:94M2I6M10I3M2I75M7I2M1I86M34I14M1I9M9I9M1I24M6I19M3D8M1I21M2I39M22D45M7D12M1D25M1I15M1I13M2D12M1I5M2I21M1D4M1260N7M3I19M cs:Z:*aagM*acgS*atcR*tctE:1*tctQ*gatT:1*cagE*actK*gccS*cttI*cgtK:2*acgL:1*gctG*gatG*gagD*actV:20*ctcK*tcgT:1*tggF:1*gctL:2*attL*ttgQ:1*attY*cagA*aatT:2*gttL:6*atcV*accL*atcL:8*caaP:3*gcaV:2*tacI:2*ttgI*aacD:2*gatE*ggtS:1*gggS:1+IF:1*ataV*caaD*ggtS:1*ggaD+GSKYGSSRMR*ttcY*aagI:1+DE:1*ggtA*agaK*ttgA:1*aaaG:3*accF*tacF:1*atcV*agaE:5*cggK*aatS:1*cgtK:4*accI:6*atcV:3*actS:1*caaS*aacD:1*gatN:4*gccT*atcV*atgL:3*tccN:1*caaE:1*tcaV*cttA:1*gatN:2*agaK:4*acaG:2*catN*gatR:1+SGFGSFS*agaS*tctG+L*gaaD:1*aaaR*attG*tcaA*gcaV:1*gtcI:3*actS:1*tctT:3*tatF:2*atcV:3*ttgM*aggK:1*attL:1*cgtK*tccQ*acaM:1*tctD*gatY:3*gagS*ccaG:1*aagF*cagD:2*gagT*tcgE:1*catC:3*ttcN:3*gctF:2*tccN*tccT*attV*cgcQ*tacP:1*actK*tctN:5*agaT:2*gagS*acaD*gctP*cctM*aacS*ctcR*atgI*gttI*aagD:2+DDAEVNDVVFEKRSKSSVSSLPSRTPTRENLRDP:1*tccP:1*gaaH:1*gagA*actP:1*agaV*ccaA*gaaR*tctP*cgtA*cagE+V*acaR*aatS:2*gaaQ:1*attA*tctA*gagP+VVPIATGVV*cagM:1*actV:1*tccT*cagG*ttcY*cagS:1+P:1*attL*gctQ*gcaV*caaV:3*gtcA*gatE:3*aatI*cagP*caaS*tctT:1*tttA*gagP*gctT*gagR*caaS*attV+VASHDD*cgtL:1*caaI*caaE*gagQ*cgtQ*gatK*gcaQ:1*gctQ*ctcI:6*gaaL:1-caaagaatt:1*ttcE*gaaH:1*cagK:1*gtcL:1+L:5*cgtA*attL*gcaL:1*gagQ*caaR*ttgI*cttQ*cgcQ*gagQ*cagE*tacM*cagE*tcaK*caaE:1+LI:1*ggcQ*cgcK*gtaT:1*gagS:1*gagQ*cgcN:1*ctgI*ctgM*gctS*ttgF*agaK*ggaN:3*caaR:2*cttV*ctaM:1*cagE:3*cagK:2*aagQ*gccK:2*actS:1*attL-acagctaccactcaatcagctactcagcaaattggctccaaggatgagttgattcaaaacttgcat:1*caaS*ataV*actK*ctaE*tggF:1*aacE:2*gagQ:15*ttgI:1*aatK:1*ttcM:2*ctcT:1*caaD*aaaD*gccL:1*tctE:1*caaE*gaaS*gccE*attV-gacaagcgtgagaaattggaa:1*gacK:3*aagS*aacA*gtgL*gaaS*ttgN*gctV:1-cta:2*gaaN*cgtN*gacN*cgtK*gctG*cgtE*ttgS*gacE*ttgR:1*aaaE*gttA:1*ggtD*ggtR*aaaS:1*gatM*gagI*attL*gacK*aagS:1+V:1*ttgS*caaS:1*agaK*gatS:1*accQ*ttgD*aaaP*ttaI*gatA*gatS*gccL*gagD+V*agaS*aacD*caaL*tcaA*tcgT*aacD:2*actN*attA:1*gctI*caaD-cacaag*aaaG*gagD:1*gaaN*gctT*ttgI*gagV:1*cagI:1*agaP*cagE+F*caaV:1*tcgL*gctL*ttcL+RC*aatP*ccaQ:1*ttgM*gaaQ*caaT*ttaI*ctaA*aagE:1*aagF*gatL*gaaR*gagC:1*gaaS*attS*ctcA*caaK:2-atg:1*gatS*gcgV*ctgI~gt1260ag:2*ctaN:2*gcaF*agaQ+EKL:7*aagQ:1*ggcS:2*gacY:3*agcD:1*gaaV +QBLJ01000026.1:100869-152175 miniprot mRNA 24989 28117 1065 + . ID=BC000019;Rank=1;Identity=0.3890;Positive=0.5629;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 671 +QBLJ01000026.1:100869-152175 miniprot CDS 24989 26779 995 + 0 Parent=BC000019;Rank=1;Identity=0.3791;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 642 +QBLJ01000026.1:100869-152175 miniprot CDS 28040 28117 70 + 0 Parent=BC000019;Rank=1;Identity=0.6207;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 643 671 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 228 111 211 - QBLJ01000026.1:100869-152175 51307 40298 40595 117 306 0 AS:i:157 ms:i:157 np:i:56 fs:i:0 st:i:0 da:i:180 do:i:9 cg:Z:29M2D12M3I56M cs:Z::1*aacD*tgtV*tcaK:1*ataE:2*tctM:1*catF*tctD*cctT*ccgE*gaaH*gcaE*tcaC*tctT*atgD*aatT*actS*tctE*aatD*gtcI*tgtS*tctD*gttK:2-gcagtt*atgI*gccS:1*ccaT*gacN:1*aatD:1*aatP:2*caaN+CYP*aagT*aagY*cgaK:1*agaS*catF:1*tccK*aagH:1*gctS:1*agcG*cttI:3*gcaI:1*gaaK*actV*actK*aaaQ*aatC:1*accN*agaT*aagS:2*gcaL*ataY:2*gagQ:1*tccL*ggtD*atgL:1*gcaP*cttS:3*gtgI:8*accA:1 +QBLJ01000026.1:100869-152175 miniprot mRNA 40299 40595 157 - . ID=BC000020;Rank=1;Identity=0.3824;Positive=0.5490;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 112 211 +QBLJ01000026.1:100869-152175 miniprot CDS 40299 40595 157 - 0 Parent=BC000020;Rank=1;Identity=0.3824;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 112 211 +##PAF 4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 212 51 168 - QBLJ01000026.1:100869-152175 51307 36943 37279 117 360 0 AS:i:143 ms:i:143 np:i:61 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:14M4I19M3I31M1I21M1D18M2D6M cs:Z::1*tccE:2*aagS*ataL:1*aagT*cttP:1*gccI:1*tatL*gaaQ+EILI:2*attN*gaaQ*ggaA*ctaT*aagR*gatN*catC*aaaA*gctG*ggtD*caaS*gatS*agtW*agtG*acgS*tacQ*atcL+FDM*cgcK*caaV*catP*ttgT:1*agaK*aagS:1*gggI*cccR*tcaC*agaK:1*cggT*acaR*aagC:6*tatF:3*ttcY:2*aatS*gttI+Y*gcaN*acaV*gatE*caaH:1*ggaR*gtcE:2*tctT*caaL:1*gcgG:1*gaaL:1*gagK*aggN*aatE:1*gaaN-atc*caaK*cttA*gtaI:1*aagN:2*tccA*acaI:3*ttgS*tctQ*gttA*ttgI*agtN*gatQ-cgcctt*cccD:1*ttcV:3 +QBLJ01000026.1:100869-152175 miniprot mRNA 36944 37279 143 - . ID=BC000021;Rank=1;Identity=0.3250;Positive=0.5083;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 52 168 +QBLJ01000026.1:100869-152175 miniprot CDS 36944 37279 143 - 0 Parent=BC000021;Rank=1;Identity=0.3250;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 52 168 +##PAF 1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1064 0 1064 + QBLJ01000026.1:100869-152175 51307 24979 28114 1773 3210 0 AS:i:2844 ms:i:2844 np:i:773 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:101M1D163M2D41M1D6M12I259M9I24M3I159M1I230M2D56M cs:Z:*gtgM*atgA*tccT*aagL*acgS*atcK*tctA:1*tctA*gatE:1*cagT*actV*gccH*cttI*cgtK:3*tctN*gctP*gatE:10*gcgQ:2*gttI:4*cacN*aagH:1*tcaG*ctcR*tcgA:3*gctG*ttaM:2*ttgQ:1*attL:6*ttaV:15*caaP:1*gctV:1*gcaR:1*ggtA*tacQ*aggQ:1*ttgR*aacS:1*atcL*gatE*ggtS:1*gggA:1*tctG*ataA*caaH*ggtA-gat:1*ttcR*aagH*ggcT:1*ggtA*agaK:2*aaaY:3*accG*tacL:1*atcL*agaK:2*cagN:2*cggK:2*cgtP*ggtD:13*gtgR*actN*gtcI*caaD*aacD:1*gatN:3*gaaQ:2*atgV:3*tccD:3*tcaA*cttI:1*gatN*ctgF:4*ttcI:1*acaS*attL*tcaK:1*gatG*aagR*agaP*tctN:2*aaaR:4*gtcI:3*actA:6*aagR:1*atcT:1*tcgT:4*attM*tacH*cgtA*tccT:1*ggaN*tctD:7*aagR*cagS:2*gagY:3*gctQ:2*ttcL:3*gctY:4*attL*cgcK:4*ttgV:4*agaT:1*ccaS*gagQ*acaD*gctP:3*atgY-gttaag*gatG:1*tcaN*tccP*gtcE:1*gagG:1*actA:1*agaA*ccaL*gaaP*tctK:1*cagP*acaI*aatS*ttgE*tcaV*gaaA*ccaS*attL*tctA*gagT*cagG*ccaA:1*gtcT*tccA*cagP*ttcS*cagT*caaT*ccaD*attL*gctT*gcaP*caaE:1*actV-ggt:5*aatT+PQGQQQLQQQQL:2*tctQ*agtQ*tttY:1*gctE:2*attR:2*caaA*caaA*gagQ:1*gatE:1*caaE*gctL*ctcQ:3*cagM*tatL*gctH*gaaL:2*agaQ*attQ*ttgQ:2*cagE:2*gtcR*caaM:1*caaA:4*attL:3*caaA:3*gagD:3*tcaR:2*cagD:2*gtaA:6*ctgI:1*gctN:6*caaR:3*ctaM:1*cagE:3*cagR:3*gccG:2*actG:1*attL*acaQ*gctQ*accV*actI*caaA*tcaN*gctT*actN*cagL:1*attS*ggcQ:3*gagD*ttgM:1*caaR*aacS:1*catQ:2*ataV*actN*ctaS*tggL:1*aacS:2*gagD*agtV:13*aacD*ttgK:1*aatK*aagE*ttcL:1*aaaL:1*caaK*caaV:9*gacE:6*aagR:1*ctgI:1*tcgA:2*gtgI:3*ggtD:9*ttgY:2*gagD:1*gttS*aagR:1*ggtN*aaaQ*ggtK:1*gagV:1*gacE:3*ttgR:1*attL:1*gatL*ctcT*accE*ttgD:2*gatA:4*aacS*caaK*tcaG*tcgA*aacD:2*actL*attL*tttI*gctS*caaK:1*aagN*aaaR:1*atcL*gaaA*gctD:2*aacE*cagA:1*agaK*cagA*caaK*tctQ:1*gctV*ttcL+DNYANRSFD*aatD*ccaS*gaaD:1*gaaK*caaL*ttaK:1*aagA*gatE:2*gaaD:6*caaE:1*atgV:1*gatS+MEA:2*gtgK:1*ttaM*gccS*aaaL*gctN*cagH*aagN:1*aatA*gaaD*acaQ:1*attL:5*caaE:2*gttK*gatE:2*acgS:2*actN:1*ttgI*gttI:1*gctS*atcV:1*ctcE:1*ggcS*ctcA*aagE:1*ttaV:1*gacE*gccS:1*tatF:1*ttgF*gacE:10*tcgT:7*atcV:4*tccT:2*atgT:2*ggtA*gacT:3*gacN:9*cacY*gctS*gagG:2*cgtS*tctT*ctcA*aatT*gacI*ttcL*actS:1*tccV:1*gctP:3*ttaN:1*tctA:2*attL*gtgT:2*gttA*aaaR*tcaD:1*gcaT*gagV:2*caaN*ttcI:1*gctS*actY:2*gctL*actA:1*gagV*gaaA*tcgV:1*gaaD:2*aaaS*tctG*ttgV*cttQ:1*gatN*agtK:1+A*gagQ*ggcL*gaaS*gtgD:1*gagD:3*gtgK:1*atgI*gccN*ggaF:1*ttgI*gcaD:2*gagT*aaaH:2*gatS:1*accS:1*ttgV*actS:1*gacK:1*gccT:2*tctA*aatG*cttT*ttgH*aacL*agcN*aacG*gttS*gcgG*aacD:5*gaaD*actR:1*ttgM*cagA*aatR:2*gccE*tccA:1*tcaE*aagS:1*tcaN*tccA*cagK:1*actN*tcgE:3*aaaR*gatP*caaK:2*tctK*atcF:1*caaV*atcF:1*tttL*gagK:1*aacH*aaaE:2*gttL*tccA:6*aatT*tccA:1*gaaA*cttA:5*attS*gctD*tcaC:1*gagQ:4*aagQ*aacG:2*tcaA*attS:1*aagR:1*gaaA*tacF:19*cttA:2*aagN*attV:12*aaaH*tctT*catP:7*catN:3*gctS:2*gttA:10*acgS*tttY*ttgM:5*aatR:2*actG:3*aacA:16*actS*cagK*agaK*agcQ*aacQ-ggcggt*agtK*ggtE:10*aatP:1*gaaV*aacL:2*gtgT:13*gccS:1*atcN*gcaS:11*ggcS:1*agaV:1*gggD*gacE*agtD*agcE:1 +QBLJ01000026.1:100869-152175 miniprot mRNA 24980 28114 2844 + . ID=BC000022;Rank=1;Identity=0.5523;Positive=0.7224;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +QBLJ01000026.1:100869-152175 miniprot CDS 24980 28114 2844 + 0 Parent=BC000022;Rank=1;Identity=0.5523;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +##PAF 4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1053 0 1046 + QBLJ01000026.1:100869-152175 51307 24982 28114 1623 3168 0 AS:i:2570 ms:i:2570 np:i:744 fs:i:0 st:i:0 da:i:0 do:i:6 cg:Z:99M4I201M1I7M1D7M4I265M1I39M5D134M1I39M2D33M1I153M2D57M cs:Z::2*aagS*acgN*atcR*tctA:1*tctV:2*cagN*actV*gccN*cttI*cgtK:3*tctN*gctT:2*actS:14*tggH:2*aagR:2*ctcK*tcgA:3*gctG*ttaI*aagR:1*ttgQ:1*attL:1*aatT:1*gaaD:1*cagM*ttaV:15*caaP:3*gcaR*gaaD*ggtA*tacQ*aggA:1*ttgV:1*tggF:1*gatQ*ggtS:1*gggA:1*tctG*ataN*caaN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aaaS:3*accR*tacF:1*atcM*agaQ:2*cagT:2*cggK*aatY:1*cgtP*ggtA:3*accM:4*gagD:4*gtgR:2*caaN*aacD:1*gatN:7*gatN:2*tccT:3*tcaG*cttV:2*ctgF:3*attV:1*gcaS*acaS*attL*tcaQ*catR*gatS*aagR*agaS*tctN:5*gcaS:3*cttM*gttI*actA:6*aagR:1*atcV*accI:5*attM*tacH*cgtV:3*tctN:1*gatE:3*ccaS:1*aagR:2*tacF*gagY:3*gctD:2*ttcR*gaaD:2*gctY:3*tccT*attL*cgcK:2*actR:1*ttgI*atcV:3*agaQ:1*ccaG*gagK*acaN*gctP:3*atgL*gttE*aagE*gatE*gaaD*tcaG*tccP*gtcG*gaaL*gagP*gagQ*actR:1*agaK*ccaS*gaaV*tctA*cgtE*cagT*acaR*aatS*ttgV:1*gaaP*ccaE*attP*tctA*gagP*cagL:1*actV*gtcA*tccT*cagP*ttcT*cagP*caaS+I:1*attA*gctE:3*actI-ggt*gtcE*gatN:3*aatQ*cagD+ALLA:1*tctQ*agtQ*tttY*gagD:3*attE:2*caaR:1*gagQ*cgtQ*gatA*gcaM*caaE*gctE*ctcE*caaR*caaI*caaR:1*tatM*gctQ*gaaM:2*agaQ*attQ*ttgQ:2*cagM:2*gtcR:2*caaM:1*cagA:1*cgtQ*attR:5*cttM*cgcA*gagD:1*tacM*cagA*tcaR*caaH:4*gtaM:6*ctgI:7*gagD:4*ctaM:1*cagE:3*cagG:6*actA:1*attL*acaN*gctQ*accL*actQ:1*tcaT:1*actH:1*caaS*attA*ggcQ*tccA:2*gagD:2*caaE*aacS:1*catQ*gagQ:3*ctaM:1*aagR*aacQ:3*agtT:3*ctcR:2*caaS*ttgM:1*cagE:1*cacY:1*aacA:2*aatK:1*ttcL:1*aaaA*ctcT:7*caaK:3*gacE:1*cgtA:4*aagR:1*ctgM*aaaR*tcgH:2*gtgI:3*ggtD:2*cgtK:6*ttgY:2*gagD*aaaR*gttA:3*aaaN*ggtK*gatE*gagD*attV*gacE*aagR:2*ttgR*caaE*attL:1*gatM*ctcA*accQ*ttgD:2*gatA:1*gccK*gagD:1*aacS*caaT*tcaG*tcgA*aacD:2*actL*attL*tttL*gctS*caaK:1*aagN*aaaR:1*atcL*gaaS*gctE:3*cagA:1*agaK*cagM*caaK*tctQ*tcgR:1*ttcL*aatD*ccaE*gaaR*ttgG*gaaD*caaD*ttaS+D:1*aagL*gatR*aagR*gatL:2*ttaK:2*ctcE*caaY*caaE*acaA*atgL*gacN*gatE:1*ctgF*gtgN*gaaS:2*aaaL*gctH:1*aagE*gagQ*aatQ*gaaG*acaN*gctN*attS*gaaN*gagG*cagG*atcS*gacT*caaP-gttttggttgatcac:1*acgA*aagA:1*actH:1*ttgI*gttI:2*atcL:1*ctcE:2*ctcS*aagQ:1*ttaV:4*tatF:2*gacE:10*tcgT:6*attV:5*tccE:2*atgS*gacA:1*ggtA*gacT:3*gacN:1*atcL*attA:1*gggE*ccaT*aatD:2*cacY:4*cgtK*tctT*ctcV:1*gacI*ttcY*actS*tctT*tccA*attV*gctE*gacN:2*ttaS:4*attL*gtgT:2*gttA:1*tcaD:2*gagS:2*caaA*ttcL*ataV*gctN*actF:2*gctE*actS:2*gaaA*tcgT:1*gaaR*ttcN:1*aaaI*tctG:3*gatE*agtI*ttaI:1*ggcD+F*gaaP*gtgL*gaaD*gagD:1*actM*gatE*gtgR:1*atgI*gccT*ggaL:1*ttgM*gcaN:2*gagT*aaaA:2*gatH:2*caaK:1*actA:1*gacR*ttaM:2*agaK*tctT:1*cttI*ttgN*aacM:1-aacgtt*gcgG*aacD:6*actR:1*ttgM*cagA*aatK:2*gccD*tccA:1*tcaA*aagA:2*tccA*cagK:1*actG*tcgD:2*tcgK*aaaF*gatN:1+S:2*tctL*atcK:1*caaT*atcT:1*tttL*gagQ*attL*aacH*aaaE:1*attA*gttI*tccQ:2*attQ:2*accI:1*tccA:1*gaaA*cttA:2*aaaR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aaaR:1*tcaT*attS:1*aagR*actA*gaaQ*tacF:3*cacN:1*cgtK:13*cttA:2*aagN:4*actK:3*gttT:1*tctR*gggR*aatT*aaaS*tctG*catL:1*cagE:5*catN*gagN:5*gttA:12*ttgM:5*aatK:2*actE*gcgC:2*aacV:6*agtN:2*aatK:3*gacE:2*actN*cagK*agaK*agcF-aacggc:1*agtE*ggtL*gagD*aatE*gatK:3*tcaA*aaaA:1*acaS*aatK:2*aacF:2*gtgT:13*gccD:1*atcQ*gcaG:6*gaaQ*attM:2*tatV*ggcA:1*agaL*gacH*gggQ:1*agtE*agcE:1 +QBLJ01000026.1:100869-152175 miniprot mRNA 24983 28114 2570 + . ID=BC000023;Rank=1;Identity=0.5123;Positive=0.7045;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +QBLJ01000026.1:100869-152175 miniprot CDS 24983 28114 2570 + 0 Parent=BC000023;Rank=1;Identity=0.5123;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +##PAF 4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1054 0 1047 + QBLJ01000026.1:100869-152175 51307 24982 28114 1620 3171 0 AS:i:2556 ms:i:2556 np:i:744 fs:i:0 st:i:0 da:i:0 do:i:6 cg:Z:99M4I201M1I7M1D7M4I265M1I39M5D134M1I39M2D18M1I15M1I153M2D57M cs:Z::2*aagS*acgN*atcR*tctA:1*tctV:2*cagN*actV*gccN*cttI*cgtK:3*tctN*gctT:2*actS:14*tggH:2*aagR:2*ctcK*tcgA:3*gctG*ttaI*aagR:1*ttgQ:1*attL:1*aatT:1*gaaD:1*cagM*ttaV:15*caaP:3*gcaR*gaaD*ggtA*tacQ*aggA:1*ttgV:1*tggF:1*gatQ*ggtS:1*gggA:1*tctG*ataN*caaN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aaaS:3*accR*tacF:1*atcM*agaQ:2*cagT:2*cggK*aatY:1*cgtP*ggtA:3*accM:4*gagD:4*gtgR:2*caaN*aacD:1*gatN:7*gatN:2*tccT:3*tcaG*cttV:2*ctgF:3*attV:1*gcaS*acaS*attL*tcaQ*catR*gatS*aagR*agaS*tctN:5*gcaS:3*cttM*gttI*actA:6*aagR:1*atcV*accI:5*attM*tacH*cgtV:3*tctN:1*gatE:3*ccaS:1*aagR:2*tacF*gagY:3*gctD:2*ttcR*gaaD:2*gctY:3*tccT*attL*cgcK:2*actR:1*ttgI*atcV:3*agaQ:1*ccaG*gagK*acaN*gctP:3*atgL*gttE*aagE*gatE*gaaD*tcaG*tccP*gtcG*gaaL*gagP*gagQ*actR:1*agaK*ccaS*gaaV*tctA*cgtE*cagT*acaR*aatS*ttgV:1*gaaP*ccaE*attP*tctA*gagP*cagL:1*actV*gtcA*tccT*cagP*ttcT*cagP*caaS+I:1*attA*gctE:3*actI-ggt*gtcE*gatN:3*aatQ*cagD+ALLA:1*tctQ*agtQ*tttY*gagD:3*attE:2*caaR:1*gagQ*cgtQ*gatA*gcaM*caaE*gctE*ctcE*caaR*caaI*caaR:1*tatM*gctQ*gaaM:2*agaQ*attQ*ttgQ:2*cagM:2*gtcR:2*caaM:1*cagA:1*cgtQ*attR:5*cttM*cgcA*gagD:1*tacM*cagA*tcaR*caaH:4*gtaM:6*ctgI:7*gagD:4*ctaM:1*cagE:3*cagG:6*actA:1*attL*acaN*gctQ*accL*actQ:1*tcaT:1*actH:1*caaS*attA*ggcQ*tccA:2*gagD:2*caaE*aacS:1*catQ*gagQ:3*ctaM:1*aagR*aacQ:3*agtT:3*ctcR:2*caaS*ttgM:1*cagE:1*cacY:1*aacA:2*aatK:1*ttcL:1*aaaA*ctcT:7*caaK:3*gacE:1*cgtA:2*ttgM:1*aagR:1*ctgM*aaaR*tcgH:2*gtgI:3*ggtD:2*cgtK:6*ttgY:2*gagD*aaaR*gttA:3*aaaN*ggtK*gatE*gagD*attV*gacE*aagR:2*ttgR*caaE*attL:1*gatM*ctcA*accQ*ttgD:2*gatA:1*gccK*gagD:1*aacS*caaT*tcaG*tcgA*aacD:2*actL*attL*tttL*gctS*caaK:1*aagN*aaaR:1*atcL*gaaS*gctE:3*cagA:1*agaK*cagM*caaK*tctQ*tcgR:1*ttcL*aatD*ccaE*gaaR*ttgG*gaaD*caaD*ttaS+D:1*aagL*gatR*aagR*gatL:2*ttaK:2*ctcE*caaY*caaE*acaA*atgL*gacN*gatE:1*ctgF*gtgN*gaaS:2*aaaL*gctH:1*aagE*gagQ*aatQ*gaaG*acaN*gctN*attS*gaaN*gagG*cagG*atcS*gacT*caaP-gttttggttgatcac:1*acgA*aagA:1*actH:1*ttgI*gttI:2*atcL:1*ctcE:2*ctcS*aagQ:1*ttaV:4*tatF:2*gacE:10*tcgT:6*attV:5*tccE:2*atgS*gacA:1*ggtA*gacT:3*gacN:1*atcL*attA:1*gggE*ccaT*aatD:2*cacY:4*cgtK*tctT*ctcV:1*gacI*ttcY*actS*tctT*tccA*attV*gctE*gacN:2*ttaS:4*attL*gtgT:2*gttA:1*tcaD:2*gagS:2*caaA*ttcL*ataV*gctN*actF:2*gctE*actS:2*gaaA*tcgT:1*gaaR*ttcN:1*aaaI*tctG:3*gatE*agtI*ttaI:1*ggcD+F*gaaP*gtgL*gaaD*gagD:1*actM*gatE*gtgR:1*atgI*gccT*ggaL:1*ttgM*gcaN:2*gagT*aaaA:2*gatH:2*caaK:1*actA:1*gacR*ttaM:2*agaK*tctT:1*cttI*ttgN*aacM:1-aacgtt*gcgG*aacD:6*actR:1*ttgM*cagA*aatK:2*gccD*tccA:1+A*tcaA*aagA:2*tccA*cagK:1*actG*tcgD:2*tcgK*aaaF*gatN:1+S:2*tctL*atcK:1*caaT*atcT:1*tttL*gagQ*attL*aacH*aaaE:1*attA*gttI*tccQ:2*attQ:2*accI:1*tccA:1*gaaA*cttA:2*aaaR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aaaR:1*tcaT*attS:1*aagR*actA*gaaQ*tacF:3*cacN:1*cgtK:13*cttA:2*aagN:4*actK:3*gttT:1*tctR*gggR*aatT*aaaS*tctG*catL:1*cagE:5*catN*gagN:5*gttA:12*ttgM:5*aatK:2*actE*gcgC:2*aacV:6*agtN:2*aatK:3*gacE:2*actN*cagK*agaK*agcF-aacggc:1*agtE*ggtL*gagD*aatE*gatK:3*tcaA*aaaA:1*acaS*aatK:2*aacF:2*gtgT:13*gccD:1*atcQ*gcaG:6*gaaQ*attM:2*tatV*ggcA:1*agaL*gacH*gggQ:1*agtE*agcE:1 +QBLJ01000026.1:100869-152175 miniprot mRNA 24983 28114 2556 + . ID=BC000024;Rank=1;Identity=0.5109;Positive=0.7039;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +QBLJ01000026.1:100869-152175 miniprot CDS 24983 28114 2556 + 0 Parent=BC000024;Rank=1;Identity=0.5109;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003123885.1_orf_scan.tsv b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_orf_scan.tsv new file mode 100644 index 00000000..24f7f797 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003123885.1_orf_scan.tsv @@ -0,0 +1,9 @@ +seg strand wstart wend aa pfam best_ref +s206 + 24968 28120 1051 ANTH:260:14-275,I_LWEQ:4:848-872,I_LWEQ:195:891-1043 sla2:1217 +s240 + 41744 42262 173 MATA:33 +s372 + 41343 41696 118 460519_cbs-7435_MATkp_MATa_MATa2:16 +s399 + 49611 50609 333 dic1:362 +s441 - 36755 37456 234 MATalpha_HMGbox:33:92-173 MATalpha1:62 +s589 - 35437 35775 113 MATALPHA2:18 +s712 - 40290 40799 170 Homeobox_KN:27:136-165,Homeodomain:53:112-167 460523_cbs-4732_MATog_MATa-MATalpha_MATa1:58 +s728 - 35331 35438 36 Homeobox_KN:19:13-35,Homeodomain:17:8-35 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha2:18 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.a2.gff b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.a2.gff new file mode 100644 index 00000000..879555f8 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.a2.gff @@ -0,0 +1 @@ +##gff-version 3 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.fa b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.fa new file mode 100644 index 00000000..9a245729 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.fa @@ -0,0 +1,954 @@ +>PPLX03000005.1:95524-162156 Barnettozyma californica strain NRRL Y-17395 flattened_line_8, whole genome shotgun sequence +GTCACAGAGCCTGCTAAGAAGACCATCTGGGAAAAGGTGAAACATGAAGTGAACCATTACTGGGAAGGAA +CTAAACTTTTGGGATTTGAGATCAAAGTCTCTTTCAAGTTGGCGTTGAAGTCTGCTGCTGGTTACGAGTT +GACCAGAAGGGAGAATAAACAGTTACAAAGAACTACACAAGATATCGTTAGACTTGTTCCATTTTCGATG +TTTGTGATTGTCCCATTCGCTGAACTTCTGTTGCCAGTCGCCTTGAAGATGTTCCCAAACCTGTTGCCAT +CAACATATGAATCTTCGGCAGACAAGGAAAAAAAGCGTTCAAAGCTCCTAAAGACCAGAACCAGTGTGTC +TCAACTGCTGAAGGATTCTATCCAGATGAAGTTTCCAAGTGCTCTTACTGAGGAACAAAAAGCGGATTTC +AAAGACTTCTTGGTCAAGATTAGATCGTCTTCAGAGCAGCCATCTAGAGAACAGCTACTTAGAGTAGCCA +AACTGTTCAAAGATGACACTGTCTTGGATAACCTACAAAGATCTCAGCTGGTTGCTGTGGCAAAATACAT +TAATCTTCAACCAATCGGCACCAATCAAATGCTTACATTCAGAATCAGAAACAAACTGCTGAATATCAAG +AGAGATGACAAAGCCATTTACTATGAGGGTATTGATAGCTTGAGCACAGTTGAGCTGCAAGCTGCATGCA +GCTCCAGAGGTATCAGGGTCAGTGGTGTCCCAGTGAGCGAATTGAAACGCGATCTTACTGCTTGGTTAAA +CTTGAGATTAAATGAGAGAATCCCATCCACCCTGCTTGTGTTGTCCAACGCATACACTTACGGTGATTTG +GACTCTGATGAAACCATTTATGATGCACTTCAAGCGGTGCTAAGTTCGATGCCTGAAGAAGTGTACCATG +TTGCTGAGGCTGATGTTGTCGAAGAGGTTAGTAACAAACAAAGATTAGAACTGCTGAAGGAACAGCAAGA +GCTGATTGAGTCTGAAACCAAGCAAGATACTGATGATGGTATTATCATTCAAGTTCAAGATAAGAAAAAC +TTGGACGATGATGAAGTGCATGAATCTAAAGAGTCTACAGAAGCCAGCAGCGAGAAAGCAGGAGAGCCAA +AGGACTCAAAGAAGGATTAGTCCTCCATAATATACATAGATCCTGTGTGTTTTTTTTGAACCATTTGTAG +TGCGTTGGAAGTTCATGTGTCATGACGTGATGATCCTTACGATACCTGTCACTACATAATGCAGAATAAA +AGAGAGATAAATAAAACGATTTGAATAGCATCATTCATGATCAATAAGATTATATCAAGAAGGCAGCGAT +ACCAATGGCAACTGCAGCACTTGGAGCGAATTTGCCAACACCGTTATCAGATTGAACAGCAACAGTTGGG +GTAGCAGTGGCCTGAACCTGGCCATCAGAAATTTGTGAAACCACAGGGGTTTCTGCAGCCGACGATTCAG +AAGCTTCAGTGGCTGTATCCTCTGCATCATCGGTATAATCCTCATCGTAATCATCAGTATAGTCCTCATC +GTAATCATCAGTATAATCCTCATCGTCCTCTCCCGTACCATCGTCTTCGTCGGCAGCATCGTCTTCTCCC +GCAGCATCAGTAGCAGCAGCATCAGTAGCAGCAGCAGCATCGGTAGTAGTGGCAGCATCAGCCGCAGTAG +TAGTAGCTGGAACTGCACCTTCACTTTGGGCGTCCGTAGCTTGGGAAACGCCAAGGACAGCTAGGGACAA +CAAGGAGATTAAAGAAGTGAGTTGCATTATTAGTGAATGAATACGCTCTTGTACTGTTCGAAAGAAATAT +ATTAAAGGTTATGCAGAGACCTCTTGGGCCTTTTATATCTTTGGAAATGTGACACCTCTCCCTCAAGATA +TCTTAATTGCACAGCTCTGGAATCTGTTCTGAGTGTAGTTCATAGCAATTCATACCAGGATTATACTAAA +ACCTTAACCCAGAATGGGCAAGTAAGTAGGCTTGGTTGTGGCTCATGAAGTTAATTCTAGAAATGAAATC +CCCGTTCTAGATGACCCCCTTGTAGATGGTGTCAGTTGCATTATTTTTTAGCTTACTATCCCCCAATCTA +GTTCAAATTACTAACATAACTAATCGTAAGACATCTGATTATTGACCACTTTTCAAAACAATACTTGATA +GAATGGCTGTACCCAAGTTATAAATAGCGTGATCGGGCTGTGTCAAATAGTATACAATTATTGTTCCGTC +GAACAGGGATCTTCATCTCCGCACTTTGCTGTGCACTTTTTGATAGAGACACGATCGAGATTTCACCATT +AAACCCTATACAGCCAAAGAACTATATCAACAATTAAAATATATTCTAGGTAGCTCTATAATAAATAATC +AATCCTTTCCGACAAGTAGGGATAGGACACTGTCAAAGAATTTGGCAATTCCTTTCTTCTCACCTTTGAG +CCTCTTCATTTCCTCCAGTTCTTGGTAGTATTGAGCCTCATGTTCTTCAATCTCTTGCAACCCAGACACC +AGATCTACTTCCAGAGGGTTTTTCCACTTACATCTCTTCAAGACCTTCCAGAAACTATATAGACCAATAC +AGACAAAGATGGCCAGATAGTTGAACAGGAAGGCTGAAACTTCCCAGTGTCCTTTGATGAAGACAGTGTA +ACCGTTGACCCACATCATAATGAAAGGACAAGTGAAGCCAAAAATACTAGTATATGGTTGCCACCAACTC +TTGAAGGTGAATTCATCTCTGTTAATACCTTGCGCCAATACCGCTTTGTAGAAAAAGTAGTAAGTGACCA +TCATCAAACAAAAATTGATCAGTTGCGACGCAGTGATCAGATTTATTAACCAATTTAAGACAACAGCGGA +GTTGTTATTCAATTGCAAGAATGACAATAGTGCCCATGCCAATGAAACAAGGACAGCTAGGATTGGGACA +CCCTGCTTGTTACAATATGTGAAGATAAATGGAGCTTTTCCTTCGAGAGCCATACCATACAGTGTTCTCG +ATGAACAATAAGTATAGGCATTCCCTGCGCTAAAAGCTGCAACAACCAACGCACCATTAACAATATCTGG +CAATACTTTAATTCCCATATTGTTCATTGCAATGACGTAAGGACTGGAAGCAGCACCTGGTCTTGACTGA +TTAATGGCTGCAGTTAAATCAGCGTCATCACTGGCAACTAAAATACCAACACATAGAGCACCTCCGATAA +ACAAGAAGGTCAATCTATAGAATATCTGCTTGAATGCTCTTGGAAGAGTCGATGACTTTGGAAGTACAGT +TTCACCAGCAACCATGGAGAGATAGTCTGGTCCTGCTATGGTGAAAGATGCCTGTGTGAGGCATGTAATG +AAGGCTTGGAAATATCCTAATGAACCAGTCGACCAGTATGGCTTAAATGCACCCGGATTCTTCCAGAATT +GGAAACCAAATGCATGATGCTGTGGGTTACCACCAACCATGGTGATAAAAGTAAACAAAATTAAACCAAC +AGCCAGCAAAACTTTACCAATAGCCAACCAAAACTCAGTCTCGCCATAAATTCTAACAGCAAAAATTGAA +ATCAAGAAATAAATAACAATCTGCACTATCAGGGTGATCACAGGGGAATAATCGTCCCTCCAGTAATGAA +TAATTGTGTTCACGGCGACAACTTCAAATGGAATCAAGACACATTCCAGGAACCAGAAATTCCAGCCAGC +CATAAATTCAAGAGCATCGTCACAGCAACGGCCAGCCATTCTTATAAAGGGGGAGTTTATCGGTAGATAG +CACACCATTTCTGCTGTAGAGACAGTGATACATAAAATTGGAATACACCAAACGGTGAATCCAATTAATA +AGAACCCAGGACCAGCCTTTTGTAGTGCACCACCAATTGCAACAAAAAGAGCAGTACCAATGACACCAGA +GATACCAATAAGTTGCACATGTCTGTTCTTTAGTTTTCTGTGGGTCTGTGGAACCAAAGTAGAGGCATCA +CTGATCGATTGTGTGTCGTTTATAGGGAACTTGTAGTCATCACTAACATGATGGTGATGTTCACCATGGG +TTGTGTGCAATTGCGTGTGTGCACGTTTACTTGCTGGAAGAGGTATGAAAACATCCTTCAGTAACTGTCC +TGTTGACTTCAACATTTGGTCGCTTAGCAGGAAAAAGAGTGTGTAATATGTACTAGTGAAAAGTTTTCAA +GGGTGCTGTATAACCCCAGAGAAAAAAATTGTTGACAGGTGGGGCGTGCTGCAGGCTGCAATAAGTAAGC +TTGTGAGTTTTCGTCGTAGAACTATTGAGCTGAACGAACGAAGAATAAGTAGGATATTTATTCTGCACAA +ACGAATGTATGCTAATTGCAATTCAGAAACTAGGATAACTGCTTGTAAATAATAGAATTCGGCTCATTGT +GGTCATTTGTTCCTGCCGAATGGGCGGAAAATTCCACATGTGATATACAAAAAAACAATGACCAAAAGCG +GAAATTATCCAGTTTAGTATGCGGTTAGAAGTCCTGTTTGATGAATTCTGTCCTGGGTAACCCGCTTGAC +GGATGACATCATACCGCGGCTCAAAACAATAAAGATATATGGATCCTAGATGCAGTTACAAAATTTTTAA +TTCCCATATCTAAATGTATTGTAATGTATACTCCTTCAAAATAAATGGAGCACACCATGAATTTAAACTA +AATGTATCCTGGCTCAGTATAGTAAAAAAAAACCAAATCTCTATAAACCCTTAATCAAATGCAACCGGCT +ATAACAGTTCAGGATATCAATGCAACTTAAAACAAAAAACGATCAAAACAAATATCAAAATCAAACCATA +ATGAAGTAAAGAAAAAAAGGACCCAGTTGTTGTGGATCAAACAACAGTATTGAAAGTGAATGAAGATGGG +TCTTCATTGAACTAAAAAGACGGAAAAAAGGCAAAGCAAAGCAATATAAGCACTCTAAAAGATATTGAAC +TCACCAATAGCCATACCAGTACCAACCATCTCACTGGCCATACCAGTCAAGATAGCTCTTCTATTCAGGG +CTCTGACAGACTTCTTGGTGGTCTTCTTGTTGTTGGCAGATGGGTCATATCTGATAGGAAAAGTGGAGAC +ATCAGGAAAAGCCAAATTGTGCTTCTCATCATGTCTGTAGTGCAAGTGCTTAGACAAAGACTTGAATGGC +GCATGTTGTTCTTCTTGAACGTTGGTGGTTTCAGCGTCTTCATCAGAAGACACAACGTCAGAAGTGTAAT +CAACAAAGTCATCAATGGTGATGTTCTCGTCATTCTTCAATTCCGCTTGGGTTGGAGCAGCTAACGACTC +ATGTTCAGAAGATACAGTGGATTGACTAGAGGACTTGTGGTTTTCGTCAATAATCTTCTTGAAATTCTCC +TCCAATGACTTGAGTTCCCCAAAAGTGTCAGCAAAGTGGAAATGAGAGTTGATTGGAGAACCAGTCTCAG +ATTCAGATTCATCAGACTCAAACAAATTGTCATTCTCCATTTGCATGAACTTCACATGAGCCAATCTTGA +AATCTCAGCGTCTTGGTTCTTTCTGAATAGAAAAGCCTTTGAGTGGCTGATGAATCTCTTGTGCTGTTGA +CTGTTTGCTGTTGGTTTGCTAAACAAGTTATCAGATTCATAGTCAGAAATAGCACTGTAGTACTCATTGT +CATCGTCAGAATTGAACAAGTTATCGTCCTCGGATTGACCAAGGAATTCCTCCTCGCTCATGGAGACAAA +GTCATCTGGGACTCTAAAATTTGGTTGGGTGTATTCATCTTCAGATTCATCATCGGCAGGTGCTTCTGGT +GCCTGCACGACAGAAGTGTTCTGAAGTTTTCTTCTCTTGAAGTTGAGGTTATTGTATGTGTTCTCATTGT +CATCAACGGTGGAAATAGATCTCAACACTGGCTTCCTGGAAACACTTGTGGTGTGTGTGTTGTTGATGTT +GTTGCTCATCGATGGTTGTACCAAATTCTTGGAGGTTTCTTCTGAATGTTTAGCTTTGGAAGTTAAAAAA +CGAACACGAGTGGACTTACGAGTAGATGGAGCCATAACAAAGGGTGGGTGGGTATAAGGGTTTAAAAAAT +TTCTTAAAAAGGGGGGGAGGAAAGTACTGAAAAGATTGCTACCTTCTGTTCAAAAAGTATCAAGAATTAG +TTTTAAACACTGATATAATTGAATTAAAAGGAAGTGCGAAGCAAATCAATTCGAATAGTTAAAAATTAAT +AAAGGTGTGGAAGGTTAAAAATCCCAAGAACCTAATGTGATATACACTATTTATATCAAAGATCACCAAT +TGTTTTGATGGGGATTTCTTGTTTTATATCAACCTGCTGATGAGCTTCCTTCAAAAAAAAAAAATTCTAG +AATTTCTTTTTTTTTTGCAGATAACTGTAAAATCTATGCAAGTTGGATCCATGTTGCGGCATTCCGCTTT +AGGAGCATCCCTGCCGGAGTTAACCAACTTTAGGCAGAAGCTCCACCTAGCCCCCACCTAGACTCATCTG +CAACTCCGTGCGGGATTCCAACGGCTTCTACAGTTTTGTTGGTAACTGAAGAAAAGTTTTCATAAACGAA +GGCACCAACTGGCCAATAAGGAGAGTGCAAAATAATACATATTAGCTGGAGCTTTGTCCGCACTACTCGA +GAACCTGCGCTGACTACATAGTTATGCAGTTCCTGACACACATACACACCTAGGCTCTCGTCCAATTCCA +TTTCCATCAGCTTGTCCCCACTCAAAAGTGCTCAAAGGTTGAGAGCAATTGAGATCAACTTTCACAACTC +TCAGCTTATTTTTTAACATAATAGGGCTGTATATACCATTATGGGGTTTTGCCCCCTTAATGACTTTTGT +TTGCTATAACGTGATCATTCAGCTAATCAATCAGCTCTCCAATATGGCATCGTTAAGCACGATGAAGGAT +TAACTTCTTACTCCGTTTCTCTGGTGGGCACACACAAATACACACATACACACAGACTACTGACACATTT +TTTTTCGATTTCCACTATGTTCTGAGTATCGAGAGAGAGCACCTGTTGTTTAGGAGAGTTCTATGGTGGG +GTGTGATGATCCGTCTACCACGGATTTGTATTGTTAAGAGAGTACGAAAGAGTATGTGCGTGTATGTGTG +GGCTCGCCTGTCTTCTAGTATTAAGTATCGCACCTCCCTATGTTAGTTTTCGATCTTTCTTACAAACAAC +AAACTTGAGTTACCATTTCGGTAGTCACAAAATAGGATCACGCACTTGTGTAAAAGTCATTACCGATAGA +TAGCCTTGTTCATGTACGTTATGGCCCAGAACATCCTCTTTGAATTCCCCTCAGTCCACTGGAGTTAAGA +ACCCTTGCTGCGTGTGATTAGGATATAGCTGAATCTATCTAATGATCTCATCTACAGCTCGGTACAATTC +ACGTGGTTAGATTTTCATTGTTAGTTGTTGCTGTTCTTGTCTTTTGTCTTGTTTTTTGGCTGTGGTATGG +TTATGAGTGGTGATTAATGAACAAAAGTCGAGGTGTTTGTTGATCTGTGATCTCCAAACCCTGTGCATTA +CTCAGAACATATTTATTTATAACCGTAGCATCGAATAGAGTGGGGTTTTCATTTTGCCATTCATTTACAC +AGTTGTTTTATTTGCCTAATGTCCCTCTGCCCACTTCTTGATGTTCACTCCTTTTCTTTGTTAAGCTTTG +TAGAAATATGTACTTGTCGAGGTATCAACTGAACATTAGAAAGAATGTTTAAAATTCAAAGGAAACTGTG +TAAATTGACTGTTTTCGCTTCTGATTGAATATAATCCAAGAATAGAAGACACCGCAGACGCTACAGATTA +GTGCTATTTTTGAATCGAATCTATGATCTTGACTACTACACCTTGGATCATTGAAATCGTTGTTAGAGAA +TATTGTTGAAAGCAGGACTTTGTAATACTGACTTAGATGCTCCGGCTCGCATATGCTTAGACTTATGACG +CTTTATACTGTACACACATGTTCCTGGCGATAAGGAGGGTGGATATTTGATTGACCTGTTGTTGTGTGCA +GTAATCCTTCGAGAAAGATGATTCGAATGACCATTACGCAAAACACCCTCTTTTGTTGTTATTTTTTCTT +TCTTTCTGGTAGGGATTGAAATCAGCACCCACAGGCAACGTGTAGGGAACGTGTAAGGAACGTGTACTAA +ATTTCACTAGTTCTTCTATGGCTGGTTAGTCCATGTGGCCCTTGTAAACTGCAGATGACCTCAGAATCTG +TTGCATGCGTAGTGGGCTACCACCCACGAGTTCCCCAACTATTGTGTGCATTCACCAACCAACGCATGTG +GGCTGCTCGAAATGAGGGAAGTTTTTTTTTATTTTGACATGTGCCACTAAAATAAAGTTGCTTTCGAGAG +AAGCCCACTGGCACATGTGCGATCTGCCCCATGCAGTGAGACATGTGCGCAAGGCACAAGTCAAAACATG +TGGCATATAGACTATAGTAAGTATAGAGATAATGGTAGAAACAATCTACTACTACATATAGCATTTATGG +AGATAGCGAAACAATCATTGTGGGATTGCTGGGCTGTATGTAGCTTATCTTCGGGTGATACCAACTCAAA +ACCAGGAAAACCCAACAAACATTCTGATATCGCCCACTCGAATTGGAGGGGGGATGATCACAGAAGGAGA +AAAAATGATTGCTATTCAGATGCACGTAGATTAGAGAAACATGTGTTAGGATGCAGTACAACTAAGCTAA +GCCTCCTCGATCATGACACGTGAAGCTCAAGAGTAAGAACTGGAAGATGGATGCAGGATATATACAAACT +CAAGTAGAACTGCAAAAACTTAAATTTTTACACGAAGTTGACAGGTGTTGACAGCAGCAGCTCTAGTCGA +GTCGGGACGACCCATTACGACCTTATCTCAAACATCAACATGATCTGATAAATACAGATCTCCTCTCCTT +TCTCATTGGCTGGTGATATCCTGCTAATCAGCACATTTAAAATTTGTATGTGAAACCTTTTCTTAACTAT +GGAGCTGGTTATCTGCGTTTGGCAATGTTTTTGTTTCACCGCCGTTGTGGCTTATCATCCACATAGATAA +CAATCACAACACCGCATTCGCCTTGCGCTTCTGGAAAGGGGAAAGATCTGTGACAACAGATCCCGATGAC +GTTCCCAACGAAGACTGATGCAGCAGACCGGTGATACCCGGGAATCCATTCCCATTCGCATCGCATCATC +TTGTCTGTCTCTGGAATTTGGTCCGTGACGTCGTGGTTGCGAGCTCTGAACAGTGAATTGTGGGGATGAT +AAGACCAGATAGGTTAGTTTCAATATGAGCAACATTAGCATCAATCGATACTTTGGTTGCTATCGGTATC +TTCGTAGATACTACTCTGTCGCATTTTCACACGGCCTATCTCTCGTCGGGCTTCTCTCTCCTTCATCATG +AGCAAGTTTCCATTTTCAGGCAGAGATAAGCGATGCTGTGAAAAATTTTGCGTGTATTCCTTGACCTGAC +CTGACCTGCATTTGCAAAAAAAAAAAGTAGCTGAAAGAGAATACATGCTACGGAGCAGAAGGAATAATAA +GATCCACAGTGCTGCGCGTGTCTATAACTACGTATATAGAACCACCTCTTTTTGTATAAGACCAGTTAGA +ATGAATCAGTCTGTCTAAGTATATTACAATACTGATTACATCAGCAAGGGCGAGGAGGTAGTCGCTTAGA +AGGGCAAAAGAAAAAAAATTATACCTCTCACTATTGACACCAAACCAAATCACAATACAGCACAACGAAC +ACCAAATACAATGGCTCCTGGTGACTTAAACCTTAAGAAATCATGGAATCCTGCTCTCATGAAGAACCAG +CAAAAAGTCTGGGAGAAAGAGCAGGAGGCACTAGCGGAACGAAAGAGAATCCAGGAACGCAAAGCTGAGA +TAGCAAAAGAACAGGAACTCGCTGAGCTTCGTGAATTACAATATAAGCAAACCGGAAAGAGAGTTGTCGA +TAGAGTTGATTGGATGTATGATGCTGGAGGTGTTGGAGATGGCAATAAATTGGCCTCTAATGCAAATGAA +GATTATCTTTTAGGTAAAAAGAGGGTTGATGATCTCATTGTCAATCCGAACAAGAAGAACGAATCCAAGG +CCACAGGATTTGATCGTGTGGCAGCTGGAAATAAGGGAATGCTCAGCCAAATGGAGGAGATTAATCGATC +TCGAGAGGATCCGATGGCAAGAATTCAAGAACAGAGAATGAAAAAGCTGCGAGAAGTGGCTATGGAGAGG +AAACTCAGGGAAGATGAGAGACAAAGAGAGAGATCACCTAAAAGAAGTAGAGATCATGGGCATCATAGAT +CTCATAGATCTCATCATAATCACTCTGATAAGAAGAAGTATGATTCTGATAGAAGGCATAGAGATTTTGA +AAATAGACACTCAGAAGGAAGACAAGGGAATGACAGAAGGGAGGTAGACAGAGGTTCTATAAGTAGCAAA +CATAGATATTTGCGCGATGGGGGAAGAAGTGAAGGCAGAGGCTCGGCACCTGGTTCTCAACCAGTTGATT +ATTAGGCCACTGTATTTAATGACTTAATACTGAAACGATCAAACTAAACTGAAATTTTGCAAAAGAAAAG +AAAACTTGGCGTGGCCAATCGAACACCCGGTAAATACCCTAAATACACACTGACACATACGTTCACCTCT +TCTGGATGTGGTCCAAGCCAGATATTTCCGGTAGGAAAACTATCCCCGATAACAGCACCCTCAATAAACG +GTTCGCGCCACATTCGGAGACTTCGGCGTTCGGATTTAACTTCTTTCGTATCTTTTTGGCACCCTGAATT +CGAAGAAAAAAGCAGTGGAAACTGAAATCTTTTCAATTCAGACTCAATCTCATACGACTCCATCGGTTCT +CACTATCCATTACAATGAGATTGCTGAAGTTGCTTGTCGCCACCGCAGCTGTGGCTAGGGCTGCTGATGT +TGCTGCTGCGGTTATCAACAGCGAAGATGAGGCTGCTGTTACGATCCAGGAAGATGCTGCTGCGTCCACC +ACTGAGGCACCAGAGCCAACAAGCACCTCCATCACGGAGGCTTTAATCGTAACAGAGGACCCATCTGATC +GTCGTGCTGGTAAGATCATTGACTATGACCGTGATGACAAACCCGATGAGACCGAGAAAGACGACGGAAA +CCCAGATGAACGTGAAGGTGATAAGAAGGACTCTAAAGATGGTAAAAATGACGGTAAAGTAAGAACATCT +GCCTTTGGATTCAGCGGTAAGATTGTGAAGTACGTGTACTTGGACCGTGTCCCTGGATCTAACTTGAAAG +ATGAGACAATCGTGCTGTTAACCTCCCTAAACAGAGGCTATGTGACACATGACCAAGGTTCCACTTGGGA +ACAGATTGCTCCAGAGGAGGAGATCCTCGACATTTTTGTAAACCCATACAATACAAACCACGTCTATTTG +CTATCCACCAACCAGAAGATCATCTACTCGACTGATAGAGCTGATAATTGGAAGTCCTTCAGAACCCCAG +CCAACTTCATTCCTGGTGTCAATCCTCTTCGTTTTCACGCTCGTCACTCCAACTGGTTCATCTACGTTGG +TCAGAAGGACTGTGATACATCCCACACAGGTGCCTGTCGTACTGTCACGTATAGAACACAATCATACGGT +AAGCGCTTCCAATTGTTGCAAGAACACGTTAACTCATGTGACTACATTGGCCATCTTGCCGAACCAACTC +ATGAAAGTTTGATTGTCTGTACCAGAGAGTTGAACGAGCACACTGTCCATTCCGAGGAGTTGATCTTCTC +TGAGGATTACTTTAAAACCTCATCATCTCCACTGTCCAATGTTATTGGTATGGTTCAAGCAGACGACTTC +CTTGTTGCTGCCACCTCTTCTGAGGATGGCTCGCTAAGAGCCCACGTCTCAGTTGATGGTAAGACTTGGG +CTGATGCTGAATTCCCATCCAATTTCAGAGTCGACAAGAGACAAGCGTACACAGTCCTTTCTGCCCAATC +GCATGCAATCTTCCTACACGTCACCACCAATGCCAGAACAGGCACTGAGTTTGGTGCTATCCTCAAGTCC +AACTCTAACGGTACTTCTTATGTCATGTCCCAGCAATATGTCAACAGAGACTCCTCTGGATTTGTTGACT +TTGAACAGATGGAGAACTTGGAGGGTGTCGCCATAATCAACACTGTGTCGAATCCAAACGAAGCACAGAC +AGGTTCTAAAAAGAAATTGCAATCTAAAATCACACATAATGACGGTGCTGACTGGGACTTCTTGCAACCA +CCAGCTGTTGACTCTGAAGGAAAGAAGAATAAATGTTTCGGCAAGCCACTAAAGGAATGCGCCTTGCACT +TGCATGGTTATACTGAACGTGCTGATTACAGAGACACTTTCTCCTCTGGTTCTGCGATTGGTATGATGGT +TGGTGTTGGAAATGTTGGTTCAGAGCTGGGCCAATACCTCGATGGTAATACTTACTTGACCCGTGATGGT +GGTGTTACCTGGAAAGAAGTGAAGAAAGGTGTTTACCAATGGGAGTACGGTGATCAAGGATCCATCTTGG +TCCTAGTTAACGGACAGGACTCCACTAATGTCTTGTACTACTCTTTGGATGAAGGTGACACTTGGAATGA +ATACAAGTTCACTGATGAGTTGGTTGTGGTCAACGACCTCTCCACTGTTCCATCGGATGATTCGAGAAAG +TTCCTTCTATTCACTCGTCTACCAGCCTCTTCAGGTGACAGAGCTACCGTGTATCAGATTGACTTCTCCC +AACTACTAAAGAGAAAGTGTTCCCTAGACTTGCAACACCCAGATACTGATGACTTTGATCTATGGACTCC +GCGTCACCCACATCAGGCTGACAACTGCTTGTTTGGTCATGAGGCGAAATATTACAGAAAGGTGCCAGGT +GCCGACTGTTACATCGGTGAGAAGTTGCTCAAGCCATATGAAGAGATGAGAAACTGTCCATGCACACGTC +AAGATTTCGAATGTGATTTTAACTATTACAGGGACTCTGCGGGTAATTGTAAGTTAGTTCCAGGCTACAC +TCCGCCAGACACCTCTGCTGTCTGTAAAGAATTTGGCGGTGATGAGTACTGGTTGCCAACTGGTTACAGA +AAGATTCCATTGTCAACATGTTCCGGTGGTCAGGAGTTTGACAAGGTTGAGGCTAGGCCATGTCCTGGTC +GTGAGGAAGAGTTCAACAGAAAGCACGGATTGAGAGGTTTCTCATTGGCTCTAGTCATTTTGATTCCAAT +TATTGGTGCTGGTGTTTTAATCTATGTTATCTACAACCATTATGTTGGTCGTTATGGCCAGATCAAGCTT +GGTGATGACAATGAACCAGTTGAATTTGACACTGACACCACATTCAACAGAATCTCATCAATTGTGGGCT +ATGTCGTTGTCGTAACAGTTGGTTTTATCAGCACAGTGACTGAACCTGGTATTGAGTTGATCAGGGAGAA +GCTCTTTAAGAAGAGTGTCTCTCTCGACTACAGCTCTGCTACGGCCACTGCATACTCTGATGACATCTTA +GACGAGGCCTCTTTGCATACTACCCAGCATGATGACACCGATTTGCTGAGCGATGGTGACTTGACAGAGG +CTGAGGATGATCGTTTGTGAATCCCTCTAATAGTAGCACCCCATGTCTATAGCTAAAGGAAAAAAGAAAA +TATTAAAGAAAGTTACGCCATAGGTATATTTGTAGGTCATCCTCATGTAACTTTTTCGACGGGAGTTTTG +AACATAATGACGGTTTTTTAAGGCAAACGGCTGATCATGAGATCTTCATTGTCAATAAAACCTTCTCTGC +ATGAAGATATTCTTGAACTTTTTTCGATAAAGCTCATGGAAGCCGACTAGCTTGCTTCAGACATAGTTTT +CACCTTTTGAGCAGTCGTGCTTGATTCTTATGTTGGTACAACTACTCCTGTACTTTGAAAAAGCATCCCT +GGGCCTACAAATGTGCCAAAATCGTCTACCGTCTATTGAAAAATGAGTATCCAGAATAGCCAGCACGCTC +ATTGCTGAAAATAAATTTGAAGAATAGAAATACAACCTCTTCCGATAATTCATTAGCTTATGCTGCTCCA +TCTGTGGCAGAGAATGAGACATTGTCAAATATTATCCTCAAGGCTCCACTTTCATCACTTAAAACACGTA +CTCAGCAAATATTTTGAACTTATGTATGCAAAACGTCTTTATTCTATCAATTGTATGTCTCTTTTTTGAG +ATGAATACTATCAGTTTCTCGCCCTTAACTCTGTACTCCTTACCAACTGGAAGAGTTTGGGCAGTTCATA +GAAGCTTGTCAAATTAATGCCAATCAAGAAGATGTATACATCATTACTGTCCCATCACTGTACCCAACAG +CCATGAAATCATTCTCAATCACTTTAGCATCCCTTCTATTCCTTAATACGATGTCCCTTCTCAACTCAGA +TCCCTTTGTAATTGACAGTGCATTTGGTTTCGTGATCTTCGCATTCTTTGTCTTAACATCGTCATTTTCA +TTCAAGTTCCCAACTATGTTCAAATTACCAGTTATCATACCCTTTGGTTTCCTAAAACTTGACTCATATT +CCAACTCCAATAACTCCTTATTGAGTTTATAGAACTTTATAGCTCCATTCCAAGATGCAAATACATATCT +GTCGTCAATGAATCCGACACTAGCAATTTTTCCTGATCCTGATCCCTTGTCGAGTGTAATCAATGATGAG +CGTGTAGATCCAATGGAGTGCAGCGCAATTTCTGGTTTAATCGATGTTGATATCACTGTGTTGTTTGTTA +CATCAAATGTCAACGATAGAACAGGGTCAGGAAAATGGTGTGTAGCGACAGAAACAACTTTGATTGATTG +GGAGCTTAAGTTTAGTTGAACCTGTGCAACGTGTCCAGACTCATATCCAATGAACAATATGTTCTCAACG +GTCCAAAGCAACTTCATAATGATTCCAAATTTCCCGCTCCTATTTACATCTCCATCGTCGTCTCCGCTGG +CTCTAATCTCATCAAATTCAGAACCTTTAATCTGTTTAAAGATATCCACAGATTCAAGCACTCTCCTCAG +TCTTCGATCATCCAACTCGCCATCACTTCGTAGAAATGACACATCATAAACATCGAATGCCTCAGAATCT +CTTGTATTAGGAGTTACTAACAATTGGTCAAAGATATCAACATTACTGAAATTCAAGGCATTGACGGGAA +GCTCATACACCATCTCTTCCTTCATAACACCATCAGGTCCAAATAATCTCCAGAACTTGATACAGTGATC +TCTTCCATGTGTCAGCACCAGGCCAAAGTAACTCCAATCAACTATGTCATCTACAACTCCCATCTGTCGC +ACAGACATGATTTGAGATGAATGTGCTTGGAATTGTAGTACCGGCCTATGCGTTGAAAGATCCCAAACAA +TCAGTACTCCTGATTCGTCAACAGACACTAGGTATGGTTTCAATTGGATTGCCTGAGTCTTAGCTGCTGT +AATCTGTTTGAATGTCACTAATGGTGTTTTGATGAATTGGAGGGACGTGATTGGGTGAGTGTGCTTTCTG +AGAGTGAACAATGGTGGTCGCGAGTCACTCGCTAGAAGATCACTCATGGCGGATAAGATTTGAAGCTCTG +ACAGTGGTGGAGTTCTTACAAATGTGATTGATTTTAGATTTCTGCCAGTGAAACTTTTTTTTTCAAGCTT +CTTCTTACTAAAAAATTGTGTCAACTTTGAAAATGGGCATCAAGAAAGTCATAGTGGCACTCCTCTGGGT +GTAGGTAACTCTATTACTACATTGAGATATTAGAGCGTCAATTATGCTTATCAAGGGCAGTAACTTGTCT +TCAGTTGCCCATTTTTTTGTCAACAGAGCACCTTCTCGCTAGACATTGGTCAAGCCTATGCAATAGACAT +ATAAAACTGAAAAGGCAATTAATTGTCTTTCATTTCTGTACAAGGATACTGAAGTCTGGAGTCTGAGACT +GTAAGACAATCCTGAAAACTCATCGAGGCAGAGAGACCATGCTGATCAAGCAAACCAGTTTTTTTTATGT +TATACGTGAACATCTTTTAGGCAGCCCATGCTCCCAAAATGATGCAGATATGCTCATCAACATCACTACT +GTGTTCACAGACTACAGACCAAAGATTGAACAGGTTGACAGAGGTTGACAGTCATACATTACAGACCTTC +CCACCTTCCGCAGTCCTATCCTGACTGCATTGATTTTGTCCGTGGTAAATGAGATTGTGCTCTTCGCCAC +AATAGAACAGTCGTTTCTGAAGAATTACGGAGGAGAAGGAGATAAGCTCGCAGCTATGTTATGAAATCCG +CATCAACTTATGCTAGAAAATTTGCCAGCAATTGTTGTTGGACACAGCACAGCCCCATCGCAACAAAAGA +ATGAAATTTCTCGCGGTGGAAAGCGTCAAAAAACCCCTAGAGATTTTAATTGAACAAACTACAATCAACC +TCGCCTTAATTAGACATTAACCATAACAATACAAAAGCTTTATCGATCAACAATGTACTTTCAACAACAG +ACTGTAGTCTAAATCAAGAGTGCGGCACCAACAGCAATGGCACCGACGGCAGCACCATAGGCTGGGGCAG +AGCCAACAACGGCCGCATTAGAGGAGGTTTCATTAGAGTTATTTTGGGCAGCAGCAGTAGCAACAATGGC +AGCAATGGCAGTAACAGTAGAGAACTTCATTATCGGCTAGTGTTTGTGTCTATGTGGTGGTATGTAAAAA +AGAGAAGATATGAAGACATTTTATCTACAATTTATACCCACAGGGAAAACTTCTCAGATTGCCAATCAAT +GTGGCCATAACATAAGTTTAATTAAATCTTGGAATCTGTACGAAGGGCTTAATTTAATTTCTCTGCTCAA +TTAGGGCAGCGCAATCTTGAGGCCCCACTGGACGTGACTGATTAAAGTTGCACCTGTGCAAAATTTTGAA +AAGCAATGTCTCTGTGCAGGAACTTCACCTCCTAATTAAAACAACCACAGTATCTTTTTTACCCATAAGA +GAACTGAATTTTTGCAATTCTAACAGACCAATGCGACAATGACGACTTTCTCAACTCACAGCTTATTGGA +GCAATTTATCTGTTCCTGGAACCTGCATTCGTCAGATATGCACGTCTCGCGGCATTTGATACCTAATCGG +GAAATAAGCTATGGACTTTTTGGCAATGGAACAACTTTAAAAGCTTCTCAAAGTATTTTCATTCTATCTT +TAGCAGGTAGTACTGACTACTCAGTGAGCAATTACCACAATCCATCCGAGACACGGATATGACGGAATGA +TTTTTTCTTTTTTATTCTTTTTTGTTCGCTTTTCCATTTCTTTCTGACGATGACGTCATTTTTATTAAAA +GGGATAGAACACCTGCATAATCGTGTCTTTTTGAAGCAACGTGGAGATCTAATTTAACCACATTCTTTCA +AAAACAATCATTGCTATTGCTATGTGCGAATTCAAAGAAGACTGGGCAAACAATATTTTTTCAACTTGTT +TGAATGGCACTGATTTCACAGCACTGCCTTTTCTTGTACACAATACTGTAGCCCCACCCTACCCCCACTG +GGGTCTCGAATATCCTGCTCAGTAGCTATCAATATAGGGACAACCCCAGTGGGGAGAGAAAGAGGCTCAC +GAGTGGAACTTTAGTGTTTAAGTGCGTTAACCCAGGGCTAAATTACTCATTATCTAATTATTGAATGCCA +TACTCAAGTGTGGTATGGTGTGTGTATTGATTTTTTGTGGGATTCTTTGCGTGGCTGTGTTACAAGGCCT +AGACGTCGATGCAGCTACAAAAATAAAAAGCTGTTTTGATACGTGAATCTGCAGCAAATTGTGCGTGGAT +TTATTATCTTGAGGTGACGAGAATTTTATCAATGTGAATTCTCGATCTTCAAATTCATCACTTCTCTGGC +AACTGCAATAGCATCAGGTGGGAAACCAGCTTTCTCCGCCACCTCAATAGCATATGACTGTTCAGAAATA +CCATCTTCTAATTTTCTATTGAAATGAAGATGTCCGCTCACTCGTTCAGCTGTAATTTTCTTAAATGCGA +AGGTCTCAATTCCGGTAGTTTGATACTTCTTGATTAAACGGCTAATCTCATTACCGTAATGAGTAGAGAA +TAGGATTCTCGACTTATTTTGATCAACCAGGTGGTTAAGTGTAGCAAATGCCACTGCTGTTCCCTCTCGG +GAACTTGTACCTCTTCCAATTTCATCCAGAATGGCCAACGATCTATCTGTGGCACCATTCAATATGTAAC +TTGTCTCTACCATCTCAACCATAAATGTGCTCATATTGTTAGATAAGTCATCCGTAGAATTCACGCGACT +GAACATCTTATCTACAACACCAATCGTGGCACGAGTGGCTGGGATATACGACCCAATCTGTGCTAGAATA +ACAATAATGGCATTCTGTCTCAAAAATGTCGATTTCCCACCCATATTTGGACCAGTGATGATCCATGCAA +ACTGGTCCGAGTGAAGTGTCAGATCGTTTGAAGTGAAACGCTTCATCTCAGCATTCAACGAATCTTCAAC +AGCAAGATGTCGCCCATCAACAATTTCAAGATCTGTCGTTCCATTAACTTCGGGGCACACAAGCCCCCTC +TCATGTGCCAAAATGGCAAAAGAACTAGTCACATCAAGTGAGTCAAGAAACTTCCCAATGTTTCTTAAAT +GGATGCTTCTCTTGATGACTTTTGCTATCAGCGATTTCACAACATTATTCTCCTCCTTTCCAATTCTAAC +CCTCATCGCATCAATAGCATGCCCCAATGATGTCAACTTTGGGTAACTGTACCATCTTGTATACATACTC +TTTTGGTTCAATACACCCTCGGGAAACAATCTGCTAAGGTCATCGTATTTACTTGTCTTTGTTCCTTTGA +TGTATACTTGATATCCCAAACTTGGACTCAGCTTCAATGACACCTCTTTAAATACACCTTCAGTCTTCAA +TTTGTCCCTCAACATAATCTCGAGATCGAGACGTTGCTGCTGTAACGCATCGACTTGATCATGGAGTGAC +TTCAAAGTTTTGGAGGATTCCTTTCTGATAACATACAGGGGTGGCGAAGTTTTTGCAGTTGTTCTATTGG +CAGTTGAGTAGCTGTAGGTTTCGCTAGCTAGAGATTCTAATTCCTCTTTAGCAACTTTCTGTTGATTTTC +CAGATCAGTGAGTAGATGTTCCACATCGATATCCTTGAGAATCTCTTTAGCTAACTTCATTTCCGATTCT +GGTATTGGTGGGAGTGACTTGATAGCATTGTCATCATTGGTAAGTTTCTCATTGATAATTTCCTTGATAC +ATTCAAAGGTCTGGACTGTTGTAGCGAGTTGAACAAGGTCAAGAATACTACCCCTTCCCATTGAAAATCT +CTGAACGATTCTGCTGATATCGTGACATTCCTTCAATCTCATTACAATCTGCTTTCTAAAAATTGGCTTT +TCAAAAAATACTGTAACCATTTTCTGTCTAGACAAGATTTCATTGACATTTGTCGAAGGTGCCATTAGCC +ATTGGGAAAGGAGTCGAGCACCACCTTGTGTGGATGTCCTCCTGACACAAGACAAAAGAGAACCCTTCGC +CAACTGGTCCCTGTATGTCTTGTGTAACTCTAAGGCATCTGATGTTCTGGAATCAATTTGCATAATCATC +TCAGGATACTGTTTCTCTGGTAACTGAAGACCTATTTTCGTTTCAGGGAAGTGTTCCTCGATGTAATTCA +ACAAATTTTTCATTGCGGAAGCCTCTTTTACTGAAAGAATATCAAATGACTTTTTGATGACAGATTGGGT +GAAGTTAAACATGCCATAGAATAGGTCCAACGTCTTGTGTCGCGCTGGTAAGTTCTGGTACTTTACAAAA +TACTTATTCAATTCTATAAGTTCCTGGTACCAATCTCCGCTTTCAAGCCTGTACTCAACTAAATCTTCAC +TCAACAGAATTTCAGAAGGCTTTATCCTCGAAATGGCTGACGCTAGACCTTCAAGTGTAACTTCTTGCAC +AAACAATGTTCCAACAGATATATCAATCCATGCTAGCCCAACAGCTATGTCTGGATCTGCTGGCCTGTTA +ACGGAATTCTTGGGAATATCAATAACCAACAGATAGTTGTTTTCCTGCTCGTTCAAAAAAGCCTCATCAA +CAAGCGTACCTGGAGATATCACTCTTGTAACACGTCTCGCAATCTTCACTGGGTCGTTATCAATTGAGGT +TTCCCTCTGAAACTGTTCTGAAACTGCGACGCCCATGTTCAAATCCTTTACAAGCACCTTAAGGTGTCTA +TCCAGTTGAGACAGGGGAAATCCAGCCATTGGTACAGTAAATTCCTTGAATGTCTTCTTAGTGAGTGTGA +TGTTCAGCCTTGGAGCAATTTCAGTAGCATGTTCAAAGTAGAGTTCATAGAAACTGCCAACCTGCGTAAT +GACGATGTAGCCCTTGTACCTGTCCATTGTTTCACGAACATTGCGCATCAGTGTGGGTAAAGAGTCAAGG +TAGAGCTTACGCTCCCATTCCTTCTTTGCATCTGGATAAATCTGTCTATTGCTCTTCAACTTGAGAATGA +CCTTTGTTGGCTCTTTGTTAGGTCTTGGACACACAGAATGGAATAGATGCGGGACTGGCAATCGTGCAGT +CCACTGTCTTCTCAGCACCAAAGCTCTTGTCCTGATCATTTGCCGTCGATATCAGTAGATATAAATGAAA +GAAGAAAGTGGTCTATGAAACTTCCACCATTCTTACAGAACGCATTGTTAAACAATAAAAACTCCTCATG +ACAGTTCGATGATCTGATCGCCATGGCATGCCCTCGGAATACCGAGTTAAAAAAATCTTTGGGTTTAGGA +CGTGTTCTTGTCCTAAATCCACCGACTACAGTTGTAGCTAAGTATGCTTCTTATATATAACAAATCATAC +TCTGTGGTTCAAGATTAATACATAACTTTCATTAACTATTTCTTCTCAAACCCAACAACTAAATCTCCAG +CAAAGTTCAAAATCTCATTGAAAATGGCCCCCTTATTGATGATAACAACGTCCTTTTCGAGGGGCTCAAA +GTTGTAACCAATCTGCACTCTGAACTTTGTCTTTTGTAAAGTGTGTGGAACAAACAAACCACAAATCACA +CTTTCTCCACTTTCATCGATGTTGTTCTGTTTGTAAGAGAAAATTACTGAACCCTTGAACTTCAAGCAGA +GTTGTTTAATCAGTTGCGCCACTGGATCATACTTGGAGTGAAGCTCAGTAGGATATGATGAGGCGTTGTT +AACCAAGTTCTTGAAGGTCCTCTTTCCTTTTGGCAAGACACCTGAGCTCAGAGTGAGTGGAGTGAACTTC +AACTTCAAGACAAAATCATAGTCAGCAAGAGACGGTGTAAAGAGCAGCTTGATTGTATCATTTTTCACGC +CATTGTTAGTAACCAACTGCATAGCAACCTTGGACAAAGCAGTTAATCTGGTTGCAATTGGTAGCGGAAC +ACTGTTAGACCACAAAATACCAGACTTGTCAATTTTCGAAGCTACAAAGAATTGCGTTCTCATGCCCAAC +GGATCCTGCTTTCTGATGTTGTCGAAGTTAGACTTGATCTGCTGATATGACTGAACAGTAATCTTATCAG +AAAGCTTAGAAACTATCTCGCTAGCGTCTTCAATGTCGTCTTGCTTGGCACTCAAATCCAAGATTAGGGG +CTCTTCTTTCCAGTTCCAACTGGCAAGAAAGTTCAAGATCTTCAAGAACCCATTCTCCACTGATGAAGGT +GAGTCAAACTGTGCTGGGTCAACAAAAGGCTTCAATGCAATTAACTCTACCAACTCTTCAGTCAAGTGTG +GTAGAAGCAGCTGATCGTCAAGCCACTTCTTGAAAAGCCTTACTGTTGGTGAATAGAATGGGAATTTGTG +TGACACAGTAGAGATCGTTCTGGTATGAGTAATGGAACCAAGATACTTTTGGTTGAACTTCAAATAGATA +GATTCCAATTCTGATCTTTTCTCTTTTCTAGCACTTTCGATCGCTCTCAGATACAACACTTCGTCACGCT +CAGTCAATACTCTCAATCTGAACCCATAGCCTTCTGGAGACAACACGTTCAACGTTGTAATGTCGAAGTT +GTAGGGAATGGAGTCCTCGTCTTTGTTGATAAAGCTTCTGTAAACAGTTTCCTTGGTCAAAATCTCATGA +ATCTTTATCAAGAACGCAGTCTTAGTATTCTCCAATGCACTGATCTCATCAGGCCACTTCACTGATGATT +CGAACTGAATAATCAAATCATTGAAAAATTCCTTGGATGAAACAGCAAATGGGACAGGAGTCACAAGTGC +AGTAGATCTCAAAGCTGGGCCAGCAGGTAGCACTGACTTGATAGCTAATGGGAGTTCCAATTTAATTAAT +ATTCTGTAGAGCTCATCAAAACTTTTGCGAAGAGCAACAGAAGCACTGGTAGAAGTGATAGACTGCTTAG +TTGATGATGGAAGATTTGGCAGTGGCAACAAATCATTAAAGTCATAAACAGAACTTTCAATCTTGATTTC +CTCGGTCAAATGCCTTTTAAAGACATATTCGACAATCGAAAGGATAATTGGTTGCTTGTAATCAGTTGTC +CACACAACAGAGTTTGAGATGGAACCATCTCTAAATCTTCTCAAAGAGCATTTGGAGCCCCAAAAAGATC +TAAACTGCTCACTATCTTCTGATTCCAAAGGACCCTTGGTGACAAGTTTCTCACATTCGTGGGAGTCAGC +CAACAGACCAATCTGAACATTCACCTTGCTCTGCTGTGAAGGTTTACGCTTGGAAATTTCGAACTCTGTC +ACTAAATTCTTGATTATTACATTGAAGCCCTTTACTCTCTCACCCAAACCATATTTCAACAGTTGTGAAA +TTTTGTTGGTAATGAAGTTCTCATATGTTAGGTGGGTAATCTTTTCGAATGGACCAAATGAATCGTCAGT +AGATGCGTCAATTTGGAGCTGTATGTTGTAATCGTATTTCAAGTGATCAACATTGGCATTCTTCAAAAAC +AACGGATCAAATCTATCCTGAATCACATCATTCAATAGATCCAACGTCTTTTTTGCATGTTGCACTAGCA +GTGCATATGAGTTTCTAGTGACTTTGTGCAAAATATTAATCTTGGATGATTTGTCAAAAATGGTTGGAGT +ATTGAACCCCTCAGAGATATATCTGCTGAGATCTGAAGAGTCGGAGTAAAACTGCAGGTAGCCAGCGGAA +GATAAATCCTGAGTAGCCAAGTATTTGATAACACCTTTAAACAGCTGGTAAGAGGAAAACCCATGCAGCA +AGATTTTGTTTCCCTGAGCACCTCCACCATTGAGGAGAGCAGCAGTCAACGTGGCAAATTCAAAATGACC +AAATCCTCCTTGTGCAACACTCGATCCCAGACCTCTTTGCGATAGCCATAGCCTTCCTAAAATACATGCT +TCTTTGAACTGCTCTGCGGATTTCTTGGCTTTATATAGGTATTTCAAGAAGTAGTCATAAGATGTCATCG +TGAGGATGGAAGAGTTATAAAGTGGGGTTGGAGGTAGTTCCAAGTCTTCCTCTTTCTGGACTCTAATACA +GTTCTTATTTGGTAGCAGCTTTTTAGGGTCAAATACTCTGTATGGGAAGCCCACCAGGACGTTGATGGAG +AATTTAGTCTTGTAGAAGTTGTACTCCGAATCAAGCTGAGAATCTGTAGCCGTCAGTCTCAGTACTGGTG +TAAGAATGTCTCCGTTGAGGTATGTGTAGGATAGTTTCAAAAAGTCGAGACCCTCTTTCTTGAAAATCAC +TCTCAAGTTCTCTGTGAGGTATCCGAGGTAGAATGAACGCTTATGCAAGGCCCTATAGTTCAGATAATCC +TTGGCCTGGAATGATTCAGTAGGCATAGTAACATTGACGTCAATTGCTAGGCCATGTGGAGATTGGATAG +CTGTTTTGAGGCCGAATGACCCCACGATAGAAACATTCTCCGGCTTCTTGTAGTCAAACTTGTAATTCAC +ATTAGTTGGTTTGGGGTCATTAAATGGCACTGTAGTTTTCTTGTTCTTGAACCATGACTCTACTTGATCT +AGTGGCTGGTCCTGAAGTTCAGGTACATCTTGAATCAGTTCATAAAGCTTGTGTAGAAACTTTTCAATCT +TGGCAATCTTGTGGTCTTTCAATTTGACTTCCTTGATCAGTTCATCAATTTGTAGCTTGAAGATATTGGA +TTTGAAGAGCTCTGCGGTCTCTCTCGCTATCTGTATATCTTGAGATGAAAGCTTCGAATGAGGCTTTGAA +GAGGAGGACTTTTGTTGTTCCTCGACATCAACTTCTTCTTCGTCAACTTCGTGGTCATCACCTCCATTAT +TCTCACCATCACTCTCATGCTCGTCGGCAGTCTTCGGGATGTCATCACTAAGCTTTGTCTTGATTAACGA +TGGCTTCTTGGAAGACTTCAGCTCGTCAGGTAGCTCTGACTTTCTCTTTAATGCTCCACTCATGATCTAG +GCCAAAACAATGAGTATTTATAGCTCCTTGCACCAAAAAAAGATGATCCCTTAACGAAATAAGGTTTGTC +TAACAATTGGCTGTGTCCAATCAGTGACAATATATACGTTTTCGCTCAGGAAATGGACCGTGTGGATGCA +ATAGTCTAGCGTCCTTCTCTATAACTCACACTGTTAAATTTTTCTTAAATTTTTCTTTGATTCGCGAAAT +TGCTAAATGCGTGAATTTTTTGGACTAGCCTTGAGTTCGCGCGTAATTTTCCTCCTTCGATATTGGTGTG +CAGGTGTTAAGTTCCGAGGGTCAAGATTGCCGAAATTGAGAACGACCGAATTGTCCGTCAACTTTGGCAC +GATAGGAGGTTCTATTTTGCGAACAACTACGACAAATATTCTCTTAGCCAACAACCATTGTGATGTCCAA +GACGATCTCTGAGTCTGATCTCCAGACTGCCCTTCGTAAGGCTACGTCTGCTGATGAGACTGCCCCAAAA +AGAAAGCATGTGAGAGCGTGTATTGTTTACACCTGGGATCACAAGTCGTCACTCTCGTTCTGGAACGCTT +TAAAGATTTTGCCTATTCAGAATGATGAAGTTCAGTTATTTAAGGCTCTAATCACCATCCACAAAGTTCT +TCAAGAAGGTCACCAATCTGCTCTCGCAGAAGGTTACAGGAACTTGAACTGGATCGATGGTTTAGGGAGA +TCTATACAAGGTGATGGATTCAAGGGCTATGGTAGATTGATTAAAGAATATGTCACCTACTTGATCAGAA +AGCTTCAGTTTCATCGGAATCACCGTGGTTTCAATGGAACCTTTGAGTACGAAGAGTATATCTCGTTGGT +GACTGTCCAAAACCCAGATGAAGGTTATGAAGCCATCATGGATTTGATGTCCCTTCAAGACTCACTTGAC +GATCTGCAGAGACTTATTTTCGCAACAATTTCACATGATAAGAGATCTGAATGCAAAATTTCAGCATTGG +TCCCTCTTGTTACTGAATCTTATGGAATCTATAAGTTCATCACCTCGATGTTGAGGGCAATTTACCGTTC +CACAGGATCTGATGATGCTTTAGAGCCATTGAAGCAGCGCTACGAGTCGCAACATGCTCGTTTGTTCGAA +TTTTATGCTGATTGCTCCTCCATTCGCTACTTGACTTCTTTGATCACAATTCCTAGATTACCAGAGACAG +CTCCTAACCTCATGGTTAAGGATGAATCATCCGTCGAAGAGGAGACTCCAAGACCAGAATCTCGTCAGAC +AAATTTGTCAGAACCAATTTCTGAGCAGCCAACTGTCTCCCAGTTCCAGCAACCAATTGCTGCACAACCA +ACTGGTGTCGATTTTTGGAGCAATCAGCAATCTAGTTTTGAGGCTGAGCAAATTCGTTTGCAACAAGAGC +GTGATGCACAAGCTCTCCAACAACAACAGTATGCTGAACAACAAAGAATTTTGTTCGAACAGCAGCAGGT +CCAACAGCAAGAACAGCAGCGTATTGCACAGGAGCAATTGCTTCGCGAGCAGTACCAGTCACAAGCCCAG +GGCCGCGTAGCTGAGTTAGAGCGCGATCTGCTGGCTTTGAGAGGACAATATGAGCAAGACCAACTTCTAC +TACAGCAATATGATCAGCGTGTCAAGGCCTTGGAGACTGAAATTACAGCTACCACTCAATCAGCTACTCA +GCAAATTGGCTCCAAGGATGAGTTGATTCAAAACTTGCATGAGCAAATAACTCTATGGAAGAACAAGTAT +GAGAGTTTAGCTAAGCTCTACTCTCAATTGAGACAGGAACACTTGAACTTGCTAAATAAGTTCAAGAAAC +TCCAACAAAAAGCCGCATCTGCTCAAGAAGCCATTGACAAGCGTGAGAAATTGGAAAAGGACCTGAAATC +GAAGAACGTGGAATTGGCTGGTCTAATCCGTGAACGTGACCGTGCTCGTTTGGACTTGGAGAAAGTTAAG +GGTGGTAAAGGTGATGAGATTGACAAGCTTGAGTTGCAAATTAGAGATCTCACCTTGAAATTAGATGATG +CCGAGAGAAACCAATCATCGAACCTATCCACTATTTTTGCTCAACACAAGAAAGAGATCGAAGCTTTGGA +GAACCAGTTAAGACAGCAATCTTCGGCTTTCAATCCAGAATTGGAACAATTACTAAAGGATAAGGATGAA +GAGTTAGAAATTCTCCAACAAACAATGGACGATGCGCTGGTGGAATTAGCCAAAGCTCAGAAGGAGAATG +AAACAGCTATTGAAGAGCAGATCGACCAAGTTTTGGTTGATCACTTAACGAAGCTTACTTCCTTGGTTGA +TGCTATCTTGCTCAGTGGCCTCAAGAGATTACAAGACGCCTTATATGAATTGGACTCTCCTATGCAAGCT +GGTAACCAGAACTCATCGCCTGAATATTTACTCTCCATTATCGAGAAAGCGTCATCCTCTGCTATGGACT +TCGGTGACTCTTTTAATGACTTCATCATTGATGGGCCAAATGGAGATCACGCTGAGATTATCCGTTCTCT +CAATGACTTCACTTCTTCCATTGCTGACGTTTTATTAAACTCTAAGGGTATTGTGCGCCTTGTTAAATCA +GATGCAGAGGCTGATCAATTCATAGCTACTGCGAGAGCTACTGCAGAGGAATCGGAAGAATTCTTCAAAT +CTTTGCTTTCCGATAGTTTAGAGGGCGAAGTGGAAGAGAAGACTGATGTGGTCATGGCCGGAAATTTGGC +AGTGCAAGAGAAACTTCAAGATCTTACCCAATTGACTGAGGACTTAGCCCCAAGATCTAATCTTTTGAAC +AGCAACGTTGCGAACTTGGGCGACCTTGTGGAAACTGAATTGCAGAATGCGGCCGCCTCCATTTCAAAGG +CATCATCCCAGCTTACTTCGCTACTCTCGAAAGATCAAGACCCATCTATCTCACAAATCGACTTTGAGAT +TAACAAAGCTATTGTTTCCGCTGCGATTGCCGTTACCAATTCCATTGAACTTTTGATTAAAGCAGCTATT +GCTTCACAAGAGGAAATTGTTGCAAAGAACAAAGGCTCAATTTCTAAGACTGAATACTACAAAAAGCACA +ACCGTTGGACAGAAGGCCTTATCTCAGCAGCTAAATCTGTTGCCCTTTCCACTAAGATTTTGATCGAAAC +TGCGGATGGTGTTCTGTCTGGGAATAAATCTCATGAGCAGTTAATTGTCGCATCACATGAGGTGGCTGCT +TCAACTGTTCAGTTGGTAGCTGCTTCGAGAGTCAAAGCTACGTTTTTGTCTAAGACTCAAGACAATTTGG +AAACTGCGTCCAAGAACGTCACATCTGCCTGCAGAAGTCTAGTTAATCAAGTACAAGACATTTTAACTCA +GAGAAGCAACGGCGGTAGTGGTGAGAATGATGTTGATTATTCAAAACTAACAAATCATGAAAACAAAACT +GTGGAGATGGAGCAACAAGTTGAGATTCTGAAATTGGAGAATGCCCTAATCGCAGCAAGAAAAAGATTGG +GAGAAATTAGAAAGTATGGCTACAGAGACGGGGACAGTAGCGAAGAAGAATGAAATAACTTATTACACAT +GTAATAGCTATATTACATGAATATAAAACGATAAATGATCAAATGGTAGGTAACTCTTCCAGTTTCGCAG +GAGTTTAAGTGCGAGCACCCCTTCATTACATTGAAAAGTTACATTAAACACTATAGCTCATGAGAAGCTG +TACTTCTCGAAAGTTCTGAGGAAGTTATAAGAGGGAGGCTCCTTTTTATTTAGTCAATGCAGAGTAAACT +CAACAATGAAGCTCTCTTTTGTTGCTTATGTTGTGGCGTCACTGAAAACATACAGAATACAGTTAGCGAT +AAAACACTCTGGACACTGACTCAAAGATAGTGCACCCTATGATTATCCTTGGACCGCTAAAACACAACTA +TAGCACCCCTTTCTTGATTTGTGGCTGTTTCGTCTACTGTAAAGAAGGAGCTTTGACGTGTTTGCTTTCA +CTTTCCAAATCGGGGATTTTCAGGAGACTGTGTTCTAACAGAATCCTGGACTTCATTATCTTTTAGATAG +AAATTAGGACCCCATTAACCGAAAATATCGATACTACTATTGATAACGACAGAATGACTTAACTGAGTGT +TTCCCATGAACCCCGGCTTTCTACAAAATAGTTTCTTGGTGAACATACAACCGACACTTGCATCCATCAA +ATTCAATAGGCTATGATTCACGAACAGGTAGCAGCTTGTTCTTGGTTGTCAAAACAAAAACAAAACAAGC +TCTCAAACAACTAATCCGAAAACTACCTCAAATATTCCCCAACCTGAATGAATTAAATCAACCGTAGTTG +CGAAAATTTCCATATCATGGGCCTTGCACATATAGTAGCCCCAGGCTTTTCTAAAAAACGCTATGTAGTG +AGTTCTTCAACGCCTATCAAGATAGACTCGTTATGCCGACCGTACAAACAAACGTGCAGAGTGTCACAGA +AATGCAATATTTACTTCACTCCTAGGCACTATATGGAAATCTGAAATAACTTAAACTCAATGAGGCACAG +TAGTGGTTATGACAGGGAGGTATCTGGCGGTTCAAGACTTTTACTAATGCATATTTTATACAGTGTATTC +GATTATGAGAAGAATCTCTTTATATACTTATTATAGGAGGTACCATATAAACTTCATTTCCCTTATGAGG +GTATGGCTTATTTCTTGCTTTCTTATTCATGTAATCAGATTACATTTCCATATTGCGTTTTAGAATTCTT +GAAATCCGGTTAATCCGACGGAATATTTTTCTATTGGCTTGAAATTGAGATGGGTATGCGATTCGTCAAC +AACGTATCAAAATTTGATACTGTTAGTAGAATCACATGTGGCTTAGGTGGAGCTCTTCGAAAGCTCAGTA +TATATAGAGATACTCCCTCAAGGGAAATAATAATAACCTGGGTTATACTCAGTATCCAGGTATGCAGGTG +CTTTCCTAAAAGGGTAATTTACTATTCAGGAAATTATGGTCTACGTGCTTATAAAATTACATGAAGAATA +CATGTATCATAAGCGTTTACATCTAATTCACTTAGAATTAAACCTTATGTTTCGGAAAAAGCGCATCAGA +AACAATCCCATCATTTAATTTCAATAATCGATGCATCTTCAAGGTGCTCGCCGTCAAGCATTGTCTCTGC +GCACTATCAATAATGTCTATGATCACCCAAGGATCGAACCTCCGAACCTCACTCAACATGGATGCCATCT +GGATACTGGATACTGGATATTAGATGGCTTTGGGAATGCTACATAGTTAGTTCATAATAAAAGCATCAGC +AAAAAGTCTTAAAGCAGGGTACCGGTTCAGATACCATAAAATGATTACACATAATAAGCTATTCCAAATT +TCCACAGAAATGCTGTACCATGCATGTCTTTCTGGAAACCAAAAGGACTATTAGCTTAAACAGATGAAGG +GGTTTAAACGTATGCATAAATTATTTCTGATCAATATCAATCTACCTATATATAACACGAACTATCACTT +TACAGACTTTGAGCCTACTTTAGAAATTTTATGATTGCAATGTATACTTCCATTTATTCATATATAAATT +CCGTATGTTCTGAGTTGGTGAGTTCATTGGCGGGGAGTGCCAAAAAAAGATTTCAAAATCTTGAACTTGA +GTGGTCTTCACGAGGGCATGGATAGCACGCGTCATCATCTACATCTAGATCTTTCCTCTTCTTCGAGATA +AGAGGCTGATATTGACCTTCATGTAGAACAAGGCTGAGCTGTTACTTTCTCGAAAGGGAAACAAAAAATG +TAGTTAGCTACTTTTTGCTCCCCTCAGTTGACTTGAAATATAAGATTTGGTTAGTGGGCAGGATTTTTCT +TCACTTAGATGCAACCTTTCTTTCAACTCTAATTGGCTTAGGCTATATAGGTCATAAGATTCATGTGATT +CTAATAAAACTTCACCTATTTTTTTGGTTTCAGACAGGTTGCGCACGGTTGAGTGCTTCCAGTGTGTTCT +TTTAGAGTGCAAATAAGATTGTGATGAGCTTGCAAAATGCTCTAAAGCTCCCTGATTTTGTAGAATCCGA +GGTTACTCTTTATTCAAATTCTCACTAAGTTAGAAGTGAACCGGGAGACATTCAGATCAACCTTTTCAAA +TAGCAGCTAAACAAGAATATCATCTATAGGAGCTTGTCGCTCCTCACGCTCTCCAGTACTTAAAAACTTC +TAAAGTTATGATTCTGTTGAGTTTTCATCTTGCTTCAGATTTGTATAGCTCATAGCCGGCTCAGCAAATT +TGGTCCCAGTATTGACAGCATAGTTGATAAAGAGTTACAGACTGTTCACTTTCCAAATGTCGAAATCTTC +AATTTGTTCTTAGACGAACTGAAAAGCTATCTATTGCATTTAGAGCAAGTGAGCTCTTGAAGATATCCAC +AAACTAATGACCAAAGATATGAGCAAAAATAATCTTTAAATATGTAAATGTCTTATCGTTTTTGGCGTAT +ATAGAGATATCACAAAGGGCAACGTTGAAATGATAAAGCCCTAGCAAATGCAGACATCTGTGCTATTGGT +TCAATATTTTTCTGATCAAATTGGATGAGTAGGAGGATATTGAGACATCAACTTTGTTGCTTAAATTTGG +GATCTGTACTTCGCTCTAACATTCGTACCCTAATGTTTGTTGTGTCTTGATACATCTAGCGCACCTTGTT +TCTTTCATTTCTCCTTAGTCGTAGGTCGATCAAAATCGTCTTTGTGTATAATTGTTTGAGCTACTGCGCA +AAGCCTCTGAGATTGGATGATATGTGCTTGGCTAATCAGAACACACAAATAGTGAACAATTGAGTGCACA +TCTTTAAGCTTAAATAATTCACTTTTGGAAACAAAAATTTGGATCATTTTGAACCCTTCTGGTGAAAAGG +TATGAGCTAAACAAGCTGCGGTATATATTTTCCCTGCTCCACAAATGGATGGAGAAATGTTCAAAAAAAC +CATCACATTACGAAACTGGAAGATCATTGGGGTTGAAATCACAACATATCGGGCTATACAATTGAGAGTG +TCGTGTCAGCAAAAGAGGCAACTGGCTTCAAACTTTGAGTTGATTTGTGCTTTGATTTTGAAATCATGCG +ATGCTGAGATGGCTGTGTAACTTGGATTGTTCCTTTATGTCTACAGTCAATATTTTCATTTAACGCAATT +TTGAACTGTTTGCCCAGCATTAAAGACAATCTGAAATGAGGCACTGGGATATTTACGATAGTTCCGATTT +CGCAAATAAACAGACCATTGGGAAATGTGGAGATTACCCCGAAAGATGACACCCACGACTATTATTCTAT +AGAGATAGATAGAAATCAGACATGAAAAGGAAATAAAGCTATTCTAATTTCGTACCTGGATGGATAAAGG +CCTATTTTGCTTTCTTGGATAATGATAAAGTGTATATGGTGTGTGAGTTGTTGAGAAAATACGTTCTTCT +AGTCACCCAATCCCTGTGTCTTGCTTCGTGCGTCACAGGGAGCAAACAAACAGTCTTTTTTTTTTTCCTG +AATAAGACTTGAGTGTTTATTTGAGTATAACCGGTAAAGATACTCATGACCCCAAAAAAGTGGAGGATGG +AAACTAACGGGTCACATGAAATTACAACGATCGCAGAAATGCCAACTCCCATGAGGTAGATTCCTCACAA +TGAAAATGATGGCCACGACACAATAGGTATACACGGTGTGTAATTCTGCCTACCATTGAGCAAAGTATTA +AAAGACGCGTCAAAAACAATATAGCGGTTGATGCATCTTGTTTTTTGATAATCTGTAAAGGGATATCTGA +CCGATTCGATTCCGTACAACGTTGGCAAATACTTTTTGAGAGAAAGAAGTACAGCCCAAATGCAATATGC +CGCATCAGCTCCATTTGACCTACTGCAATTGTCTCACATCTACGCGTGCACCTTATTTGAGGCAACCCAC +GTACTGCAGAATACTGCAAGAGCTGAATATTGATTACCCTTTTCGTCACTGCGCCCTCCAACAGAGAGGT +GGCATGGGAAACCAGAGGACTGCACCAAAAGTAAAGATCCCCAGAATTCTACTATGGTGATGAGCTATTC +GAAAGTTCCTAGAAGAAGCATGAACAGTTCAAGAATAAAGATATTAAATATTCTTATGGCCCAAATCTCA +AGTAGCCCCAGGAGTTTTCTGGTGAGGAAGCTGGGATCGGGATACTCACGAACAGTTCATAGTCAGGTAT +CTCGATGGACGTTCCACCTTAAATATTGCAGATCTTGGCGTCGGTTCCTTTCGCGCGTTTACAGAATCCG +ACCAACGCGCTTGGTAAAGAAAATTGTTTATTCTGTAAACTGCCTAGTTTAAGCAGACACTTCTATTAGT +GAAATTCTACTTGCCAGTTTAAATACAAACATACTTCCAATCACAGATACACAACATCCGCCGAAGATAA +TGTCTGTTTCTGACCAGCATATTAGTAAGCAATAGAACCTCTGACCTTCTGACCTATTTCAGAAAAACTT +ATCGTTGACCTCTACTTCTGACCGGAAGAACATATTGGAGATACTGATGACACACATAGAAAGAATGCTC +TGATTGGAAGCAATGGCAAAACGAAGGAGGGAGCATTCTGGGACTTAGATTAGCCGAACTAGCGTGCCTC +GTTGCAGAAAAGGTGGCGCCATAGACTGCGATATGGAAGATGACGCGTCATGATAAAGAGTTCGATTAAG +AATCGATAAACAGCAATTGGCCGTGATGGTAGTGGTATTGCACAAAACCAGCGACCAAACCAATGACCTC +GAAGGGTGTCGGATAGATTATAACTTTGCATCAATGGACTTAGGGTATCAATTATGTGTGGCGATGTTTC +CTGTTAGATATATTCTGAGATCGGGTGATAAGTCAGAAGCCTACATTGATAAACCAGCTCTAATTTCGAG +GTGAGTCTATCGGGGTTAAAGTGCCTGATGAAATGAGACTTAAAACTCTCGGTGGTAGATCTAGGTTAGG +TCTAAGCAATTTTAAATAATGTGAAACTGCTGATAAGATTCATCTAAGATCTGTTTCAATTAACTGATTG +CTCAGAAGCAAGCCATTCAAGTTCGCCTTTATAAGACTGAACGGAAAAAGGATAATGTTCATGTTGCACA +TCTTCGTTCCACCATTTAGGGCTAAATTAGTGAGTGAAAACGAATCTGGGTTATGGGCACAAATTCCATA +GATACGGATGGGCGTCCTTTAACGTTGCTATCACAACCTCAATCGCCAGGCAGGGAGAGTCCCTTCTTGA +GTGGTTTTAATGCAATTGCCAGTACGACCATGCGATGTGGACCGTCCTTGGATGAGTTGCATGTGCCAAA +CACTACTAAGGATATCATAATCCTTTAACGCATAGCTCAGGACCTTGGTGAGGAAGACATATGTACTCTA +CTCTTTCGAAGACTGGGGAGGGATAGATCCTACCACAAAGGTATACGGCAGCTAAAAAAGACTATTGCAC +TTTAGGTCAGACCGTTAGGTCCGACACTAACGCTTATTATTGAGTGTGGACTTCTGGGCGCCTTCTATAT +TCGCCAGTAATGTCGAGTCTTTGAACAACACTTACTAGACCAATAATTCGAGAAGAGTAGCAACAGTGGC +GGTTGGAGGACTATCCAGAAGAGTCTCTAAAGTGAACGAACTCCGTTGATTAATTCCAGACACAAGTGGC +TAGGGAATCTGATAAATCCAGCAATAAAATGTCACTCAGCATCAGTAACTGATCTCTTCTTGATCGAAAA +ATCATCCCACCTCGGAAAGTTTGGAGGTGAAAACTCTTCCATGAGAAGAATCACAACGAATCAGATTAAA +ATCACTGTTTATCCAGGCAATGAACCTAGGGGAATTTGACAGATGCCACCAAGATTAATTTTGCTTGCAG +ATAAATGGTTTACAGAGTTCCTATAAAGGAGCTGGATTCAAGAATGTTGAATTTGACTGTTGATGACGAG +GAGAGATATAGTTGGCGAAGGCTACGATTACTAATGGTCTTAGAGAAGGGTAATATTTGAAGTACCTGAC +ATGAAAAAAAATGGAGGAAAATACGTTCAGTATGTGAGATCACTTCTTCAACCACGGGTGGCGTTCACTC +ACTCCATTGAAGTATATGGAGACTGCGTAGAGGTCGCTCAGGCAAAATTACAGTGTTGCAAGTTTAGAGA +AAGTGGTGTCACCCCATTAAAATTTTTGCAGGAAGCTGAAATGAAGACTCAAAGCTTATATGTTAAAAGA +CATTCACCACGTCCTATCATGGAGTGGAATATCATGAAAGTGATAACTGAAAAAGGATTGGTTGAGTTGA +ATTCTTTCATGAAAGGGACAAAATTTGTAACAACAGAAAACTACAGTCGGGAGAAAGCAAATGAAATAGA +TCAAATAGTGATTCTCAAAGCTTGTATCTTTCAAGCTGCCATCCTGAAGGTTTCAAAAGTTCTGATGCAA +TGTTAACAATTTCAGAATTTCCTAATAATTTGTGCAAGGAGAAGTGTTCACATATTGGAGTGATAGGCTT +AGGGACAATCGTTTTTAACAAGTCCCGACACTAGACATGAAATTGAATCCCCTATTTTGTCCTCTCGTTC +CTTTCTTCTATCAATGTTGTTAGTACTGTACCGATTCATTACAAACTTTGAAGTGCACACATACCTCTTA +TTGGAAATCCTAAGTATAGAAGTTAGTTCATGCTATGATCTCTGACAATCTTACACATACCAATTGCGAA +TTTGGCCTTTAGTGAGACCTGTCTTTTTGACCAAAGACAGAAGCATTGATTCATTCAAGTTGGGCTTCTC +CGGATGACTATAATACCTAAACTATGTTAATTGTAAATTTGAATAGGGTACTGTGCGAGAAAACATACCA +ATCCTCAAGAATGGCAACTTTCTGCCTTGCTAATCTTTTCTTTCTAGAAATAATACTTCCATCTTCTCTA +ATCATCTTCTGCGTAATAATATTGAATCTAAAAGGGTCAGGATTGTTAGGAGATTCTGTCGAAGTTGAAA +ACCGCTCAAGGGCTTCGACTAAGATGGCCTTCTCGGAGAGCAAAAGCTGAGATTTCAATAAGTCTTTTTG +AATTTCTTGAAACTGTGACTGATCCTCTGCTGTAGTATTTGAATGTCTTTTCTGAAGTAAATCCAACTCC +TTAGTGTGTTAGTTTGATCGCTTGACATAGAAGTAAAACTTACCATATGTGAGGCTAGGATGTTCGAAGA +AAGTGTGCCAACATCAATGGAGCTAATAAGTGAGCAGCTTTCTAGGATATGTTTCGAGCTCGAGGATAGT +AAATAGCGTGAACTTATTTAATTGTTAGTACCAAATCTGCATTGCAAGAGTTTCAAAAAGAACATACTCT +GCAGTATCCCCCTGTTGTATATTATCAGTGTCTGAGAGTAATGAAGTTATTGGACATCTGCTAAACTGAT +CTTCAGACATTGTTTGGAGGCAGGAAGTGAACTTGTAGTAATAGAATAACAGGGAGCTAGAGAAAGTTTG +TTTCAAGACTGCTCAAAATCCCTTGACAGCATGAACATAAATCACGTGATAGAGATGAAACAACTGATCA +TGCAACAGCTAAAGAAACGAATATATATACACATATAATCGCTAGAAAAACTAAGATCTTAGGTGCGTTT +TTCCAATATTTAATAGTAAGAATGTAGGTTATCCCTATTCCTTATAATGTTCAGGTCAACTTCAACTGTA +ATAGATGGCGTATTCTTTCACATAACTGATAGCTTCTGTTTCAGATTAAAAATAACAATAACCGCCGTAA +CTGTTTGTTTCAAAAAGATAATCAAAGTGGGGAAATAGCTTATCGACTTAGGGAAAGCGAGGAGCCGTTC +CTGCTGTTATAAAGAAGCACTCAAACTTTGGAAGTGACATTAACTGAGTTTCATCAAGGATAGGTGTAGC +TTAAGGTTCTAATAATACTGTTCACAGGCTGAGGAGACGATGGCTTTGGTAGAACATTACTAGATGCTAA +CCCATTTGCATCATCCTACATTGACGTTCCTGTATCAACTCTCGAACTAACTGTTTGGTTACAATAGATC +AGTCAATGTATAAAAGTAATACAACAGTAATTCAATTGTTATAAGCTTCGATTCAATTATCGGTTTAGCA +TAAACTATATAGAATGCTCGAATGCTTGTCCTATTCTTGGATCGATCACAGATACATCAAAACTCATCTT +TAGAAACTGGTGGTTCTCATCTGCCTCATTTGTATTGAAAACATCTTGAATTCTAGAGAATGTACCTGGA +TCCTTGTTGTCATAAATGTTTTCAGAGATACAAGTAGGCTCTGATGTTACAAGCAAAAGCCAATCGAAAA +AGGGAAGGCGATCACTCAAAACAGACAAATTGTACTCTGTGGAATATCTCTTCCATACAAGTTGGATTTC +TGGATTCCTCTCCCATTCTTTCGCCAATTGAGAAGATAGGACTCCTTGTTGATCTGTTGCAACGTTCCTA +GAGAAGAAGGATCGATAGGCCATAAAACCATTCAACTTTGTCCGCTTTCTTGAGGGCCCTCTCTTTCTCC +TCAAATGTTGGCGGATGTACGTACTACTATCTTGACCAGCTTTATGATCCTTTAGTCCTTCAATAAATCT +TTCATAATCGGCTGAAAGCTTTGGTATCTTTGGTAGGGACGTAGCGGCAAAAGTAAGACACTGAAAATTC +GAAGGGGAGGTACACTTTGACGGGTTCTTACTTTCAAGGGCATTATTGCGATGTTTCAGTCCAGGAAGAG +AGAAGAGTTTAAATCCAGCACTGTATGGCTTCTTCGGGTTGCTTGTCCACTTTGCTTTCACTTCTTTCCT +AGGCATTGTTTGTCAACGTGAAAAACTGAATATATGTATATACAGCTGGTTAATTTCATACCACTCTTCA +ATAGATCGTGTCCGTCAAGCCGGAAGGTTAATCGTTAAGCCACGCCTCAAAAAGGTCTGCGTTCGCGAAA +CAGTGCTTTACAAAAAATTCTTAATGGAAATAATCCTGAAAATATCAATAGAATCTCTAGTTTTCGACTA +ATAACCATACATATTGTTCAATGCTACCCATTTAGTTTGTCCATTCTTCCAACGTCTATTTTACAAGTTC +CATTTTGACAGATGCTAATAGTAGTAAACTTGCAGTAGCAGCAGCACTTTAACTTCAATCCATTCGGAAC +ATTTCAAACGTGTTGGTTCGTATTCGTAACGACTATCACATCACGCTCACAAGTATCTCTAAGTATATTA +ATGCTCTGCCCCCTATAACAATTTGGGAAGACTAAAATATAGAAAACACAATAATCTCGAGGTGCTCCTG +TAGCTTGCCCTGCCTATGCTTTGCATTGTATCGCATGTTACGAATGTTACGTTTGATGCTAATCATCCAA +TCTCTCTTGAAGTCTGACCGTAAAATAGCCGCTTTTTTAATAACCTGATCTGATACATTCGTTTCTTAAC +ATCACCGTATATCAATGCAGTCTTCTCCTTATGAAATCATAACTCTACACCTTCTGCTAACTACGCCTGT +ATCCTGCCTATCCCCACTCATAGTTTGTTACCGTACAATTAATGATTCTATACTTTGTGGTTGTCGCTGG +GAAGGACTCACTTTCTCTGGCGTTATATTAATCTTGACGAGAAGCTTTGAATAGAAGGACTTTATTAAAA +TCGACATCTTCTTGATGATCTCATATTCGCCACCTTTATTATTCTCGCAGCTTCAATGAAACACTGTAGA +TCTTCTTAGCGTTACTTCTTGGGCGTTGGAGAATTTCTGCCTCTTCATAACATCCAATCTTCCTTCGACC +TGATTTCCAACCATCAAGGGCAAGAATTAAGGCCGGAAACGGGAGCTACAACATTCCACATTTGGCTTCA +TTCTTAATATGTGAGTTTGTTTACATCATATTAAGCTCAATGGTTTATTCGGGTTTCACCTGTTTAATGG +GATTTGTTGACTACCTCTATAAGTTTTCAAATCCAGAATTAGATTCGACGCAGGATTCATCACAGCTTCG +ATAACAGTTTTATAACCTTGCAAAAGCCTGGTTCATCCCCAGCTGAGAATTTACAAGCCATTGAGGATGA +GAGCACTCCCTTAGGTGCTAGTTAATGTGTGTAGTTTTTAGTCTGATTGTCAATTCCACGTGTAATTCTC +ATCACCTCTTTGGGGTCAACTTCCGAGGGCATATATTCCCGGAAGTGAGAATACCTGGACCCAGCATCAG +AGCTAGTGTTTATCTTAACGAGTAGAATGTCAAACTATTTCCTATGCAGCTCTATGACCCAATGGCTGCT +CAAAAAGGATGGCGTCCTGTTCTTTTAACGACACCAAAAATCAAATTCTGGTCGAACGTTCTGTGATTCG +AACGAGATGTAGAACTTTCTTAAATACGACTGTATGCTGTAAGCCGGGCGGCACAGAAGTTTGTTTGAGT +GGCAACCGGCGAGCAGTAAGGACAGGAGCATCAACTATTAGGTCATGTAGATGTTAATGGGTGCATTAGT +AGTTCCTGTACCGCTCGGGGCGCAGTAAAAACAGAACTTATTCTACAGTATTATTCGCAATCAGCTCCAA +ATTCCACAATTACTACATGTTAATCAGCTATACAATACGGCCTTGGTACAGCCGTTTGAACGAAATAGGT +GTTAATTTAAATTTAACTCTGAAGAGAGATCAGAACACCTGGGCTGCGTTCAAACAACCCAAATAAACTG +TCAGGAAGCCGCAAGGAATTTGAGAAGAGTTGAAAAACCTAATAATGTGAAATTGGCTTGTTCTTGAACT +TGAAGCTTGGGCGCGCCTCGATTGTACTCGGAGAAAGTTACAAGTAGTAAACGTAAGAGGATTGGCAAAT +AGGAAGGGCGGGAAGGGCAGTTTACAGGCTTAACTTAAAACTATTTGGGGGAGAGGGAAACCGAACAACG +TAGCTGTTTACTATTTCCAGCAAAGGCCATTCAATTCAATAATGGTACACAGATAAAGATAATAAATAGT +GTTCAAAGCTACTTAAAACAAAAGTTAAAGGAATATTGGAGATGAAGTTCAGAAGTCTCCCAATCGAGAT +GCTCATTACGAAGATTAGTGCCCAATGAAAACAGATGGTTAGAGTGTCACATTGTAAGCCACTAGCGAAG +ATTACTAAACTAAGTATACTTAGAGCCATATCAAAAGTCTCTAACAAGAACGAGTAATGGTATCTATGAA +TGGATACTTCGAGATCGAAGTATCTTGTATTTAATAAACATTATATACATAGTACGATAGACGAAAGATG +AAGGTTTGTTGGTGTTTGCAAGATAGTTAACCTAGCTCCACTTCGATATCATTGGGATACATATAATGGT +TGGATTTAAAGACAAGAAAGGTGAGAGTTTAACCTGTCACATCAAACGCCCACTAGTCTCGTCATAACTT +CGAGTGTTTCACCAGCAGGTAAACTATTATCAAGATATGATTCAGAGATAGGCAAAATTTTCCAGATGAT +GAAACCCAAGTCAATAATGTTCTAAGCTATTAAGGTAAACAGAATCGATGATAACAGTGGCAAAGTAGGA +AATGGTAAGGAGATGCTATTGATGGCTAGTGAGAGTCACAAAAGACATGATGTTTGGCTATGAATTACAT +TTGAAACGCGCCCTACAAATCAGAGGCAATAACATGAACTTATATTCTGGTCTTGGTTCTCATACGCTTG +TTGGTGAACCACACTCTTACTTGAAGTGCTGTCATACCGGATTTCTCTGCGATTATTGCTCTTTCCTTTC +TGGTTGGATTTTTAGTAGTTTCAAATGCTTTCTCCAAAAGGCTGCGAGCTTCCTTGGAAATATGTCTTCT +TCGCTTCTTTTGTTGAATATTGTTATTACTGTCTGGTGAGGCCATAACTGCTTCAGAAACAGAACAGACA +TTAGAAGTATTCATAGATGATGCTTCCGGAGGAGAATGTGAAGATGGAGATATGTCTGAACAGTTAGATT +CTGTTGCGCTTCTTTCGTTCACAGAAATAGTTGAAGACTTAACCCTGTAAGACTTGCTAAGAAAATATGG +TTTTTCAACACTGTAGTCAATCAGTTCTAGGACCTCAGAACCGTACTTGTCTGCCAATGTGTTAAATAAA +AGCCTTTGATACTGACCTGTGATAAGTTTCTGGTTCATGCCCTTAAGAGACTTGAGAAGTTGTTAGTAAA +GCTTAGTTGGATCAATGGGAGTTTACATACTAAAACTCGAATTAAAGATTCTTCAAAACCATCCAAGAGC +TTCGCCAAATAAGATCTATGGGCCTTAGGAACAGCTTGTAATGTTCTTGATCTATTAAAGGGATCAGTGA +CACTAACTTCATCAACCTCGTTTCTAGAAGGAGGGACAATATTTCCAGCCAATTTTGAAACATCCTTTAT +CAAAAGGGCAAGTTGTTGCCTAGCCTGTGCGAGACTGTTTTGCGAAAAGAAGTCATCAAAGTCATCACTT +GAGTACGACATTGATTGAGTAGCGGGCTATGATAAGAGGTTTGTAAATTTAAGGTGTCAAATAAAAGACC +CACAAGGAGTTTACGATATAGTGATCTAAGTTACAAATTTTGATTTTAGTTAAGGAGTTCTAGTTCGCAA +AGAAAGATAACGTAATCAAGGTTTTAGATTTTTTTCCTACCATTGTTTAACCACACTCGAAACTGTTGCC +TCAAAAACTAACAGACATCCTAGAAGTGAGATTTTTTATGTTTAACTTGTTACTTTAAAATATCTGCTAA +TATACTCGTCGAAGATAAGTACGGTACTAATAATGGAAAGTCTTAAGATTGCACATTTCAAGGCCAGTGC +TGGCAATGACTCTGATTTGCGAATTAAGAAAAGTATTCTCCCATTTGTTTCTATTCCTAAGGGGCCTTCT +AGTGGCCAGAAGTTTACCTTAACATTCGAAGATTCACCTTCTACTTCGAGCAAGCAATATTCGAATGTTG +CTCGTCCTAGAAACCAGTTCATAGTGGCAAGAATGATTTTGAATAAGATAGTCCTTGCGAACTGCAACGA +TACGATTGCCAATGATGACGTTTCTAAGATAATATCGATAGTATGTAATTGAAACACCTCTCCTGCACGG +AGTCTAAAAGTTACTAACATGCTTAGATTTGGAAAACTTCTTCTTCACCATTTCAAAAATATTTCAAGTA +CTTATCCCTTCTTGAAGAAAGTTGGCACGAAGAGCATTATCCACTTTACCATTATCAACCTAAGGTCCGA +TCCAAGACAATTAAAAGCTTAGCACCAGTACTCGAGCAATCGAGAACTATGACTGGGCGGATAAGCAAAT +GTGCCCCGTTATCTTCTTTGAAGAAAAAATCTGGGTTTGTCAACACAAGTCCTCACATGATGGGCACCTA +CGTTAAATTATCAACTAGCTCTTTTAGACACTCAACTGTTGTTTCAAATGTGAGTATTAATCTTGTTAGT +AAGTTTAGACTGACCAAGGGACACACTTCAGGAACAACATATAAGAAGCGTGGGCCAAAGTCTTCAAAGG +ATGATGCCTATATAAAAAATAATCTCTGGTGTCTCAGTGGCTTTGACAAAACAAATTATTTTGAACCTTT +GTTTTACGAAGATAAGATTCAGAACATAACGCTAGAAGATTTTAAGGATTACATATGAACAATCTACAAT +AGCAAAATATTAAACTTTGATCTGTAAGCCCCCACTAAAAGACAATGGAGAATTTGAAGTTGCATTTATA +TTTCAAGCGGTCAGTACTCTCTTGTTTTTTTGTTTACTGCATTATGGATTCATAGGTGATCAGAAACTTT +TACGGCATTGTGTGCTAAACTCAGACAACTGTAGCTTGTAAGGTTTCTCCGATCTTGATTTCTTCCACCA +AAATTCAGTTGCAGTATGGCATGTAATATTATGGCAGCCTTTACATTTATCAGTTGAAAAAATGAAGACA +CTTTCGACGACAATCGATAAACTATTGACCATCTTTTATGTTAGCCCTTCCCAAATTGGCAATTTCCGCT +TGTTTATATAACTGGAATACTAACCTTTGAAGGCTGGTTCTATGAGCAATGCATAGGACAAAATACGAAT +TCTGAACAGCAATCACGCAACTTCTCTAGCTACTGCTTGCACTTGTAGCAGCACAGGCATATCGATCTCA +GTAATCAGAATTATGTCCACCACTTTGGCTGTCCTGATTATAAAAGCGAAATAATTTACTGCATTTAAAG +AAAACTTATAAGAGCCGCGAGATCTGTGGTCCTCGAGATCTGTGTGATTCTTGTACTCCATATGAGAACA +ACCACTCAATCTTTAGTCCACCGGTATCCTATTACCTGAGAAGATATCACTTGAGCACCCCGGGGGCGAA +TGTTAGTTAGTCACAAAACAAGTCATGTTGGTTCAAATTGGAAAGCATCTGCGTGACTGTCTCTGTATCT +TGACTCTCAGCTTATCCTCCACATTATGCCACACTCGCTGTTAATTCATCGGCTTTTAACTTCCTTTAAC +TTTAATATTGCTCATCTCACATTCAGTAGGCAGATCAAATTCTTCTATTATCACTCCTATGGGGTTATAG +GATTTCTTATCTAGCTTGGCAATCAGAAGGATCTTGACCTCTTGACATTCTCTAAAAGTATTTTGTTAGT +ATTATAGCGCGGCAGCAAGAGCCATTCATGTTTCTTGCTGGCACTATTCTTGCAAAGTAAAACTTTCTGC +CTGCTGTGTTCTGGTTTGCGATAACGACTCATAAGCAGCTGTGCTAGTTGAAAAGATGTGGACAAATCAT +GTAGCTCCGGAATATTTACACGAATATTGATGGGTTATCCAAATTCCTCTATGCAGTTGGTCCTCATGTA +CTCCTCTAATTGTTGAAGATTCTTTGCTCTAAATTGCCTATTATTAGCAATGCGTTCCTGGTAATGCCAA +ACATTCGGAAAGAGTTCACAACTTGGAAGGATTTGAACTATAGGGGCCAGCTTAAAACTAGCAGCCGAAT +CTCGATTTATTCAAACACGCATGTGGTGGTTCCTCCTTCCACAGCGGTGATGTTCGTCTCATTTTTCAGG +GCATTAGCAGCTGACAAGAATTCAATTCGTTCTGATCCGCGGCTTTTTAGAAGATATACGCATGGGACTA +TTATGCTATTCACTAGCAAACTCCATTCGGCATGATCCATGTCGCTTCTCTCGTATCTGAGGCATAAAAT +TACTGCCACTCCGATTTGACACTGTTTCACCTCTCGTCTGCCGATTAATACAAGGCGCATGTATACCAAC +AAAAGTCCCCATGATCGAGCACTGAACCTTTACTAAATTAAACCAAGTCCTTATTTTGAACATACTCCGG +ATAAAACGTCAAATGATAGCATGTTCATCGATCCCTGTTATATGCAATTAATCACCTCAGGTATAGTACG +GGTGCTTTTGACTAGGTCAGGGCAAACTTCAGACAATCCATGGCTTTGGTGGGTACGAAGAATAAGGACG +AATAGGAAAGGCTTCTTTGCGTATGCAGGATTCAACACTAATATTAGCAATTACTTCCACACTTTGTATC +CTGTAATGAGGGTAACGAGCGTCAAAATCCTGCTGAGAAATACCTACTCGTACGTAGCAATTTAACTAAC +GTACCATATAAGCACAAAAAGTACAAACATGACCCGTGGAGTTGGTTGGCTTTGGCTCAAATTAGTCATT +CAGCTATTTGTTGTGGTATGAATCAATACCAACAGTAGGTGACAGAACGCAGGATCTTTAGTCCCGAAAG +GTTAGGTTTACCAGCTTTCAGGCACATCCTAGCCATGATATGGTTCTGCGCTTCGAGGTCTTCTTGTGTA +ATACAGCGCCGGAAACACTCCATTTCAACATGAGGGTGTTCCACCGCGTTGAGCTAGCACCATTGCCTTC +AGAGTCTAATATTTTGGAATCGGTTGCTTGTTGGAAAGTTTTCTTGTTTCAGTTATTTTTGGATCTAGGA +GAGAGCTAAGGCAAATCGATTGAAACCTATCTCTGTGTAGATGAACTGGTGCTTTTAGAACATGTGAAGA +TTCAAGGATGCTACTGGAGGTCTCATTAACTGGTTTTCTTTCTTGGTCGCGGTCATGCCTTGGACTATGA +TTCTGTTTCGGGTTTTCCCTCCAAGTTAATCTCCTATTAACCCTGATAAATTGAATCTTAAATCTCTTGA +TTTCGCCCTCTAAGCTATGAGGTACTGCTCCAGCGGTCAAGATTTCCTTATTCTTGAAATAAATTTCACA +ACTCGAATACAAATCTGTGTTTCAAGTGGAGAAATAGGAATTGTAGACGCATACTGTTGTAAATGTTGAT +ACAGGTAGAGTTGACGGCGATTCGAGCCATGGTGCACGCAGATCGACGGTGTCGTTAAAACTCGTGGAGC +TGTTGGTGGTAAAGTGTGATTTTTGTGTCAAGTTACATAACCGCTGACAAATCAACACGTCCGATTCCTT +CCTCAGGTTTGACATGCTCTTCGACTTTTGGAAACCAAAATCTAAAAGCCCAGTGGTCAAAAGTGCTATC +CACAAATCCATGCCGAAAAGGCTTCCTTTTGTATTGAAGCAGCATAGTATCCTCTTTGGTATTATCAGGT +CCCGAACGGAACAGCCAAAATAATCCATGCGGAGTAATTATCCAGAATCATCACATTGCGATGATCTACC +GGTATATCAACCAGCATTTGGTATACGGGCTTCAATTTTACGTTTGCCGTAATAGACATAGCATAATTGA +TAAGTCAATCTTCGCTGTCTATAAGACTGCTTACTTCGGCTTCTTCAATGAGAGTAGGCTCAGTGATCAG +TTCCCCTTTCAATGGGTATGGAGTGATCGTCAGAGTCTCACGTGTGGGAAACCTACCGCCTCTAAGTCTG +CATTGCACCTCAGGATAGTGATCTCTTTTGAATTCAAATCTTCTTAAACCCCTCTGCTTCCAAAATCAAT +CTGATCCTGTTTGGGAGTTCCAGGTATCAGATATTAGCTTTTGGATGTTTTCACTTGTCATATAGCTATC +CATTTCACGTAAAATTCTTAGCAAAGCGAACTCTTTGGCTGTCTGTACCCTATTTTGTTTGTGCGACTAG +GACTGTACGCGTTAGAAACGCGTCTTAAAAAAAAAATACTACGACTTTCATTCTAAAGTTACCCGCCTAT +CCCACGAATAAACGCGCCCAGAGGCCACTGATAAAGATCACGTGATAGATTTTAATCAGCAGCAATCTAC +GACCAACGTATATTTATGGGGCAGAGCCAGACAAAGTACCTTAAATCTCCCAGAAGGGTGTATGTATGTG +ATCGTGGTATTCACAATCATCAGCTAAATATATGCACCTATTACATCTTAATTGCTACCTTGGAACCTAT +CAGTTGGCAGGTTGGACGGCTGGTAGTTGTAGCTCGCCACAGCAGCTATATATTAGCTCAAAGATGTTTT +CAAGAGGCACGCACCAAACGTGAGTTGTATTCTCACTGTTTCATATTATAATGCAGTATCCTATTTCATT +GTTAACTTGTTTGTATTGTCTGACAAAAATAAAACTTCTCTTCCCAGCCAACAGCTGAGACAGTAAGAGC +AACCGCTCCAGAACTGATTAGTTACTTTAATCGAAATTTAGTGGGAGCATTTTGAATCACCAATCTTCAA +AGAGAAAACGTGTACCTTTAAAAAAGCACCTGTCCAATGTACCTACCTCCTTAAACTATTATTTCAAAAA +AAGGATATGAAAACAAACTGAAACAATATCGATATATTATCACAAAACTTGATAATTCGATATGAGACAA +TGAAACAATGTTTTGTGGAGCGATGCTGATATTAAAAGAGAACAAGTCCCACTAAGTCTCTGCTGTAGTG +GTTCATTGCATTAAAAGGCTATGCTGGCTCATACAAGAATCCAGAGAGCCAAGTGCATTCAATCAATGAT +GAATTGCACTTGCAAATGGATAGCCCGATTCAAATCCACAATCATCAAGCAGACTATGTCCACGTCTAGC +CTGACGTCAACGAGTATAACTCTACAGGCTTTTCTCGATGATATGAAGGCCAGAGGTAAAGATTTGGATA +ATAGTGCGCATATTCGAGGTACTTTCTATGAACTTTCTGTGAGAATGACTTTGATGGATGCCCTAAATCT +CAAATTGAAGAACGTTGGTGGGACCAGGGACAATGGTGCTGACCTGATAGGTTTTTGGAATATGTCTGAT +TTTGCGAAACCTCAAGCCAATCCAGTCCTTGCCCGAGAATTGGCCCGTGTCATGAGTCCACTGTGCCATG +AAAATCATGATCGTGTGAAATTTGTGGTTCAGTGCAAGAATTACAAGAGGCTTATTTCCCCTAAGGAAGT +CAGAGAGTTGTCAGGTATATCCAAATTTCAGGGAGCAATTTCTGGCACTCCTGTTGTGACTGTTCTCTGT +GCTCCCAAGCCACTTACACGTGCAGCCCTGCTTCAATTCCGTCTAGCAGGAAAAAGCTTTATTTTCATTC +AGATCAAGCCACATGAATACAAAGGATACGGAGACCGCTATGATTTAAAGAACTACAAGTCTGGCACTAT +TAAGATCGGCGGTGAGCATTCGTTTGCTTGTCAGTTTGAGCAAGACAACCTTGCGCTTGTCCGTTTTCTA +AAAGAGCTTTTGACAGCTCTTTCGATTGAGGTGAAGGAAATTCCATTGGACCTGGTTTCAAAGACTAAGC +TTTGTAGTGCAATTCTATTCCATTATCGCTGGGCATGGGTCAGAATTCGTCATCATAGGCATCTGATGGT +TAAAGATGTTGCCTATCGTGAAACTGTCTTCAAGCTGGAGAACACGAGAATGAGTTTGGAGGATCTTCAA +AATAGGTTGGATGAAATCCCGGAGGGGTTCCTGGAACCTATTGAACAAGTTATCCAAACGCTATTAGAGT +CCCTCTCTCATGTGAACACTGAAAAGACAGAATTTGGAGTCTCCGATATGATCACCGAGATACCTGGTTT +GGACCATGGCAAGATGAGCCTCTCCTACACTTTAACAATGAAAGCAGATGCAGATAGATGGAGAGAGCGT +ATCAGAAGGTCATACAACGATCCTTCGACTCTAAAGAAGTATGTTTTTGGATTAGTTGACAAGATTGAAA +GGGTCCCAGATGGTAAGTTGTCTCATGATACTACCAGTATGGGCGCTTCAAATTTCGCACCTCCCTATGT +GACTGAGAAATCTTCAGACAGTGATTTCTCCTCTATTCCAATTATTGATGCGTTTGGTAACGCTCCTTTT +TTGGAATGTCCCAAACATTATCGAAGCAGTGTTTAACATACAACTACGCAGGGGATAGAATCCTCTAATG +CCTTGGGTCACCGCGGAGCAATAGTAAAGTTCGAAGGAGTCCAGCGTGAGATAACGAAGCTTGAAAGTAG +ACATAAATCGACCATAAACTACACAATGACGCTTGTTTGGGAGTTTGGCGAGCTTGGAAAGTCCCCACCC +GTTAGGTTTGCAATTCAGGTGGCCTTGAGTAAGTAAATTTTAATTTGGGTCTAAAGTAACTAGGTCAAAA +CGATTTTGGTTAGTGGAAATATCGATTTCGTCATTAATACTCGTCTTACATTTGTCTTTATCAAGAGAAC +CGTTAAGTGGGGTTCCTTCTTTCCCCTCCCGGATTTGTCCGCAGTTGGACTTTGAAAATGTACTTTTTTT +TTTTTGGCTTATCTTTTACTTTGAGATTTCCTACCCACTGGTTGGTTTCTGAAAGTCTATTCAAGTTGAA +CTTACTCGAATTCTGAAACTCTGTATACTATAGCTATGATTCTTAATCTTTGATTCCTACTGATTTGGAG +TTATCAAGATCTCCCTCCACAAATTCAGCAAAGTCAACTATCCTTGATTCAACAACATAGAGTAGTTTAC +CTGAACTCTCCGTGTCGAAGAATATCGATTATCGGGAGTTGAGACGTTTTAAGTTTTCACCAAGGATAAA +TTCTCCCTCGTCATGGGCTACTCCAATTCCAAAGCGCACATTGTGATATCAGGTCGTCTCCTCGTCACCC +TCCTCTTTTCTAACTATCGAACTTACCCGGACCATCTTTCTTATCCCCACTACAAAACGGTGCGAACCAT +GATGCCTCAGATACTCAAATTACGGTTTCAATTTGGCAATTTCAGACCCAGGCATAAAGCTCACTCATAC +ACCTAACGCATAAGAAACTATCAACTCACTTAGAGATCCATATCTTTTTTACTTCCTAATTCCCATGCAG +GCTAAATAAATGCACGTTTTATGTTGAAATTAAGCCTACCATAATGCCTTTGTCAGCCATTACAAAGCTC +CAACAATAGTATTACAAACATGTCTAATATCCCTAATAATATTAGAAGAAGTTAGTGCTAGCAGTATAAA +TGTTATAACTATAGAAGAAGTGCTTTTTAGCCCAAGCTACCTGGAAATTTTGAGCTTATAAGGGTAACTA +CCCAATGTACACACGCACGCATCAAATGTAAACGGATATACCGTCGGCTTTAAAAATCAATCATAGTTCA +TATGCGACACTCTGATTTAATAGAATTTAAAAGTATAGACTTTGCAATTGCCATATAAGCAATTATTAGC +ATATCCCCACGAAATTCATGAAGAGAATCTACAGGGACTGTTCCATATGACCCTTTATTAAAGATCATCA +TTAAAGAACTCTAGTAGAAAAATGACTAAGAGATATCGATGAGGTAGAGTTTGGATAACAACGAACAGTT +TTTGAAGATTTGAAGCTATTAGTGCAAAGTCGAAGACCAGTAGAATCCTAAAACCTGATTATTCTGAAAC +CAACCTGTCTATCGCATTTATAGTTTTATTGTTATAGTTTTAACATAGAAGACACATAGTCTGCAGTGCT +TTAAGAGTTCTCTTTATTGTTTTTACATATACTATTTCGCTTGTCGCTTGGGTTTACTACTATTGGATCT +CAAAAATAAAAAGAAAAAATACCAAAGAGAAAAAAAAAAAAAATTCCTATCTCAATTGAAACCCAACCAC +ACCAGTTCCAGGAAGTCAACTCGATCGCAACGGGTCAGCCATGTCGTCAGTCACTGCTACTACCCATAGC +ACCGCCACTGGGGCTCCAATCGCACTGCAACCTGCAGCATCCGCCAACGCTAAAACCATAACGGCCACCA +AGACCCCAACCACCAAGTGGCCCTTTTGGTATGGTGGTTTCGGAGGTGCTGTAGCCTGCTTCTGCACCCA +TCCTCTAGATTTGGCTAAGGTGCGTCTACAGACCGCTCCTTTACCCAAGCCATCGCTATTTCAGATGGCG +TTCTCGATCCTGAAAGCAGATGGGATTACAGGTATCTACTCTGGTCTTTCTGCAGGCTTATTGAGACAAG +CTACCTACTCACTGACCAGATTCGGCGCCTATGAGTACTTGAAAGAGAGTTTCGTTCCAGCCGATAAGAT +GAGTTCAATGGCTTATCTATTGCCCATCTCTATGGTTAGTGGAGCTGTTGGTGGGATCGTTGGTAATCCA +TCTGACATTGTGAATATCAGAATGCAGAATGACACTGGTTTACCGATCGAACAAAGAAGAAACTACAAGA +ATGCGTTTGATGGTGTGGCCCGAATTGTGACTGAAGAAGGTCCTAGTGCCCTATTTAGAGGCTTGTCTAC +AAACTTGGTGAGAGGTGTTCTCATGACTTCATCCCAAGTTGTCACTTACGATTTGGCCAAGGAAATGCTC +ATCTCCTTCGGAATGAAAAAGGAGTCCTTCTTCACATTTTTCAGTTCTTCTCTGCTTGCTGGACTGGCCG +CAACTACAATTTGCTCACCTGCTGATGTGCTCAAAACAAGAATCATGAATGCATCCGGAACAAACCATTC +CGTGGTGCACATTTTGACTAAAGCTTTGAAGGACGAAGGTCCCAGCTTCATGTTTAGAGGTTGGGTGCCA +AGTTTTGTGAGACTTGGACCAAATACCATCATCATTTTCCTCGTTGTAGAGGAGCTGAAGCGTATGAGAA +TTGGTTTATGATTATTTATAGAGTGTATATCTGTTTGAAAGTTGAATTATCATTGGCTTTATCAGTAAGG +TACGGCTGTACACATCCGTTATCGTCCCTTTTTTCTTTCTCCTCCCGTTTTTTCCCGTTTGTTTTTCCCG +ATCTTATCCGTTTAGGGGGAACTAAACAGCCGTACATCTTTTTTGGGCCTTCTCCACCAAATCTTAGAGC +ACTGAGGGTAATAATAAAATTCGCGAAATTATTACCACGATTTATTACCTATTACCGGTAATAGAATGGG +AATCAGTAGTGGTGAATAGGGATTTTTTGGAAAGTGCTAATAATTAGTGGTAATAGAAGGGTAATCAGTA +GTACAGGTACTAAGGATTTTTAGAGAGTGGCAATAAAATGGCAGTCAGTAATACTCGCAATAGAGGAGAG +TCCGGTAACAATAATGGTAGTACATTATGGTAGTACATTATGGTAGTACATTATGGTAGTGTCCTCCCAA +AAAGAGATAATTCAGGTAGAGTTGCAACGAGTTGAGTAAAATGCTCAACTCGCGACTGCGATGATTAAGT +AGATATCTTGGGCATGTATAGTTGAGCGATCTGGAAATCGACACTTTCGAAATCTAAGCAAAACTTGTAA +TCACCGATAATTTTTCCAACACCTCGTTGCTGTTCTTGTTTTCCAAGCTTCCTCGCTTTAAATGTACCTA +TCGAAATCTCTAATTGGAACCCATCTGTCAGCAATCCATTATCAAACCGATTGAAACATAACCGGTCAAT +GTTGAATCCAGAAAGACGTATAGACTTAAATAGTTGGTCCAATTCTGTCGAAATGCCTCTAGTTTCTGGG +ATTATGAAATCACATGGCAATTGCAATTGCTCGCTGTTCGTGCCCATCAACTTCAGATAAGGGAAGAGAA +GAGAAACAAAGACAATATTCAAGCTCCAGTAGGCGATAATCTTGTTGCCTTTTCTTCCATTAGGAAGCCA +ACTGCAAATAACTGGTGTTATCCCAAAAAGAAGGAGTCCCAATAGGCCCAGTGAGAAGAGATATACCTTG +ATCTTTTGTTGGAGTGATAAATATTCAATGATAGTTGATCTGGAGAGGAGTTGCTGCATAAATTCATTAT +TTGACGGTCCCACATTGTTCAAAATGTATGAAATGTTCTGCACAACTTCAAGATCTGGATTCATCAGGCG +TTTCCTTTATCTAAAATGATGTGTGATTGGTGATTGTAATATTTCTTAGAGACTCTTTAAATTACGTCTG +TGATTTTTTGAATGATGCAATGCAGCATAAGCGATTAGTCATTCAAGAAGCTATGTGATTCCAACCCCAA +AACACCTCTCTCTCTTGTATGGTGCCATCATGATAAGTATATAAAGAGATGTTCCCCCTAAAAGAAATTA +CATGGTTTTTTTATACAATAAGCATCAGTGAGAGTCCTAAAGTAGGCTAGCACGTCAGATACTAATCTGC +TATAACATTACACATAGTCAGGTTATTCCACATTCTGAAGTCAAAAAAAAGAACGATGAATACGTAGTTT +TTGTGAAAGTATCTACCATGTGCCGATCCATACATCTAGGTATCGACTGTATGCATTCTCAATGCGTTGT +TGTACATAACGGGCGGCTAAATCATTTCTAAATCGCATATCAATCTCTACACATCATATAATCCATAAAT +AACGTCATAAATACAATAAATACTATATCCGGACACCCATCTTTTTCAAAACATCCTCTGTTGTCTCCTT +CTTCCAGTCAACACCTTCTAGAATGTCTCTGATCTCATCTCCTTCAAATTGTTCCCCAGATTGAACAAAC +ATCACTGTGCCATCGCCTTGAACAGCGCTCCTTTCACTGATTGTTCTTTCTTCAAGATTACTCAAATGTG +TCAAAGTCACAACATGCACGTCAGCTCCCTTCTCAGTCATGGGAGATATGGAGGAGTGTGATGAAACAGA +ATGTGAAGAGGACATTGGAATAGATGAAATAAGATCCTCGTTGACATAATGGTAATTACCAGCCTCTTCG +ACTTTGATTGGTTTGAATGGAGGTGTAGTAAAGATACTCGAATCAACAGCTTGGAGCTTGTTAACAGATT +CAACGATTTTTTGAAGTCTCGGAGTGGTGAAGATTCCATTAGATGAAACAGATAACAATTGTGAAGTGAT +TGACTCCAATGCCGCAGTGATCTCATGGTTTGACTTCAATTTATTCAGAACCTGGAGATTTACCATACTG +AGTTCAAGAAGGAATCTGTTAATGAACGGCATGTCCAATTGCATATAACTCAAAAAAGTCTCAAGCTTGT +TAAAACAGATGCTAGCCTGTGTGGTAAATATTTCAGAATTGAATTTATGTGATGGCTTCAATACTGGCGA +ATGCTTGATACTAGATTGGTTGTTGTTGTTGGTTCCGAACAGTGTGGGTATAGTTACCTGCGACTCCTCT +TTAATGTTGACAGATGATGAGAGGGATTCATTTCCATGAGTTGAGCACTTCTGGTATTTCTCGAGCTCAA +TTAGCTGTAATACGACCTCATTAAGAACATAGACTGGTGATCCTGGTGTCTCATCATGTAAACAGGCTTC +ACGTTCAGGATCGCTCACATTAATATCCTCATAACCGCATAGAATCTTGTTCAAAATGCTTGTGAAATTC +TCAATGAACTTGAGATCAACATTTTTCTCAAAATTGAGTCTTTTTCTGTTATTATTAATGTTCAATTTGA +TAAAGGTGATCAACTCATTGACATAGCTGAGTTGCTGGACACCGTTAAGGCACGTGTTAAGGAACAAGAG +TTGCAAAAGTTTCTTCAAAAAAATTTCAGAGTTGAAAATCTCCACGTTTAGCAAGGAGCAAAGTTGTTCC +TTGTTGAAGACAATAGTTTGTTTTAAATCATGGGCAACATTGCTCTTGAGAATATTCGATGACTTGTTCA +TGATCATGATGTTCAAATTAGAAGAATACGTAGAGGTATGGAGTGACTGGTTAAGCTGATTGATAATGTT +GTAGTAGGAAGACAGGTAATCAGTCATGAATCTCTCAAAATTGATTCTGATATGAGGATAAAAGAACTTC +ACAAGAATCAGCAGCTGGAATTTGTTCTTCTTGAAAAGCTCTTGAACGCTAAACCCCAAATCAAACCAAC +GACCTGCACCGTAGGATGATTCATCTCCACATTCAAACAATCCTGTAAATAAGTTAAGGTTTTTCAAGCA +TAGCGTCAAACGATGTTGGAACTGAGAGATTGAAACCTCTTCCTGCTCTATGATAAGCAGGTCATTATTC +AACTGCAAATTCATAGAAACAAAGCGTCCATCCGAAAGCTTGATGAGATTAAGATTATCCTCTAATGTGT +AACTGTGTGTCTCCTCACTATCATCGTCTCCTGAATTATTTGTGCTCTCCTGGACAGGAACAAGATTCTG +CTGTAACCTCCACTCTTCGAAAGAGGGTAGCTGAAGTTTCAAATCAGTACAAAGGTTTGTATAGTGGGTA +TTAAGTTGATCAACTTCGTTTCTAATAAAGTCAACTTGATAGATCAAGTCTTTCAAATTGTCTGATCTTT +GAGGTGGTAAAACATAGTCGATTTTGCATGATATCTTCTTCTTTTCGCAACTGGAGCATGGGTTAGGTGA +CTTGGTAAAGGCGTCGCACTTGATCTTGTGTTTACGACACCGAATACAGGACTTTAGTAGCTTAGGCTTG +GGGAGCACGAGGTTGTCAATATCAGTGTGTTTCAAGGGACACTTTGCCGCCTTAGAGAAATGCATCTTTA +AACCTGTGTGCCTATTTTATAACAGAGAACTGCTCGATGTGACAGTGTCCTGTAGTCATTAAAAAGCGAA +TAATCCAAGTGCAAGAGGTGAGGATTATATTTTGCGGCTATTCCATTGGCTAAAATTTGCAGCCCTTCTC +CAGGAATTGATGCAAGCTGTGATTACGTCAGCCCGAGACAAAAGAAGTCCGAGAAAATTTTTGACGGGTT +TGCGCCAAAAACTGCCGGAATCGGTCCATGAAACGCAGAGTGGAACTTTGGGCCAATCAGAGAGTTGAAG +TCCATCAACTACAACCCCTCTCAAGACCCCACCATACCAGAACGCTCCATGAATAATCTTATATTACCGA +CTGCTTTTTTCTTCCTTCCCTCCTTTTGCTTGACTTAACCCAAGAAAGGTGTGCATCTGCTTGGTATAGC +GGACCACTCTTTCCGAAGTTCGGATTCCATCAATTTCACTGCAAAACCGAACATCGGATTAACCAAGCTA +AACGGCGTCCGCTAATTTTTGAAACTTGAGATGCCCCGTTAACCGTGCATGTTCGTATTTGGAACAGTAG +GACTCCTAATATCTGGTCTTACCTTCTCTGTCAATAGAGTAATGCTATGTGGAGCGTATATTCATTTTCA +TGAATACCTAAGACTCTTCTTGAGTTCGTCAGTCTAACCTGAGCCATAGACGCAATTGGACTATGGACCC +TGGTAGTTAGGGCTTCGCTGCACCCGGATCCCTGTACATCGTGCGTTGTCGATCTGTCACTCAACAAACA +CCGGATCAGTGCAACTACTACAGTCATTACTTTCTACAACGGCGTAGATCATCTATACAGAGCTGATGGT +GGAGTTACTCTTAATTGACCTTCTCATCGTAGCTGAGATTAGTAGTGGTCCGTTCATCTCAAGTCTCCTG +CTTTATGTTTTCAACGTTCCTCAAGGACAGTGTCGACTTTCATACTATCATTCAAAGCATCCTCATTTGA +TTCGTCCTTCGTTTCATCTCCGTCATTATCCTCACCAGCACTCTTCTTCTTCTTTCTTGATCTCTTTCTC +TTTCTCTTCTTTTTCCCTGTCTCAACAGGTTCTGTGTTCTCCACTGGGAGTGCACTGGTGTCCATGTACT +TTGACACCAATGGCTGACCACGAACAGCAGTGTCAACACCATTGCCGCCGATATCAGTGGTCGTGTCACT +GGATTCCGCAGGAGTAGAAGTCAGTTCACCTCTGGTTGTGCTTGGTTCAGAATAAGAAACATCTCTCAAT +GCCTTTGCAGGCTCATCGAAGATCTTCTTTGGGTCTGAATTCGAACTTAGACGTGATGAGGAGGTCGAAT +TATCTGTTGCCTGTGGTTTTGTAGTTGTTGTTGATGTTGTTGATGTTGTCGATGTTGTCGATGTTGCTGA +TGTTGGCGCTGTAGGAGTTGTTGGTTTTTGTTCATCACGATTGACAGAGGAATTCCTGGAATTCGACACT +TTCGAGTTGTGATGATGGTTGGTGCCAGAATGAGATCTCTTGTTAACACTATCTAAAGGTGTGATTCTGT +CCTTGAGAGGGATGGATCCATTTAGTTCCTTTCCAAGTGAGCGAATTCTCTGTTCGATAGTCACTGGTTC +ATTTGATCTATTCAATCTTGAAATCTTGCGCTCATCTCTCTTGGTCACCTTGATAGTGCCAGACTCGATA +TCTGGTGTTGATGAACGCGATGGCTCTGTTGATTGAGTGAACGGCTCCCTGCTGAATGGATTCTCGGACA +TGTACTTTTCCAATAATTCCTTGGTATCAATCTGAGCTACTTTGCCCACTTCTTCAAACAATTCCTTTGA +TGAATTGATTAAAGCCTTAAGTTCAGCGACATGTTTATTTGAGCTCTTAGTCAATTTGTCTATTGATTTG +GAATATAGCATATCCTTCTTGGTATACTTTTCCTCCAGCTCTTTCGATCTCAGTGTGATGGACTTCAACG +ATCTGAAAATAGTAGTATCACTCTTGTTCACCAAAGTGATGTTCTTGTTTATGAGGTTTGTTTTGTCCTC +CATAAATGCACCCAATCTCTCCTTCAGTTCCTGAATCTCTTTGTTGTAGAAATCTCTATATTCTTTCTGA +CTGCTCACAACTGTATCATATGATATCTGAAGCCTTGATACTTCGGCCACATTGCTATCCAACTCATTCT +TCACATTCTCAAAGTTTTTGAGCTTGAAAGCCTGATCGTTGGATATGGACTCCAAATTGCCTAGTTGCTC +CTCTAAGTCATTGATACGTCTTTCTTTTCTTTTCAAGAGTGAATTAACAGTATCATTCTCATGTTTAGCA +TTGGAAAGCTGTTCCATAAGTTGATCTCTCCGTTTCTTCAGCAGCTCATTGCTCTTCTTGAGCTCCTCAT +TGTTGTTCTTGAGTCTAAGGTTTTCAGATTTTAGCTCGTCCGCCTCAGCATGCAGTTGGTTGATGAATCG +GGAAGATGTGGAAGTCTTGTTGGGACTTAATGGTCCAACAGGTGTGGGCGTCTGTGTAGTCGGCATTGAT +GTGTGCTGTTGGATGAAGATACTGTTCTTCACGGGAGATGACGGCGAAGTCATTTGTGGTTAACTCCAGT +AATTGTGTGTTCTATGAATTCGTCACTTTCAGAGACACGATGTGGAGGAATTGGATCTCGTACTTGTGTC +TATAATCTCAGTATAATGAAAGCCGATCACAATGACTGGCCAATGCGTTTTTTATAGATTTAGGTGATCA +GTGTTCGATCCGTTTACCAACTCTGGCCTCTTTTTTTTCTCGCGTCGCCGTTTTTTTCAATTTAGCTGAA +AATTTTTGTTTTTAACCATGACAACAAGGGTAGATCTAATGGGAATGTAAAACAGTTTGACAGATCAATG +TTTTAACAAAGGACGCATATCGAAGCTGTGTATTGCATTAAATGCCATGTTTAACATTCTTTGGATCTAA +TAACTAGCGCTAGCCGCTTCCTGTATCCAATTTCGACGTAAACTAATCTATATTTGTTTGTTTACATTTC +GCTTTTAACATTCTTCTTGACCCTCATGATTCAAAAAACGAATAACAAATTAAGTCTATTCAGTGCGTCT +CTTCTTCTCCGCAGGTACTTCATCATTATCACCATCACTCAAGTCTAGAGGTTCACTGGCATCTGAATCA +ACCTCCTCGTCTAGGCCATCCCTCTGCGCCTCTGTGTAATACCTCACTGCATTAGGGAAAATATCATCTG +CAAACAATCTTGCCAAGTCATCGCCAGAGGGAAATTCTTTACCAGGTTTTTTCCCTGTCCATTTAAACCA +TCCGAAGAATGACTTCATCCCTGTTCTGTAGTTTTTTTTTCCCTCTGGAGTTTTCTTATCAGTGATCTTC +TTTGGATTGATTGAGTCGTATGACTTTGGCCACACGATGTCGACAGCCTCACTAGTGAGACGCTCTGCAA +TAGGCTCCTCCTCTTCATCATCATCATCATCATCACCACCAGCAGTACTTCTATAACCTTGAACAGCATC +AGAAGGGTCAAGTTTTTCTAGTTCAAACTTCTTCACAATCGTTTGAGCAGGGAAGTCATCATCAAGCGCA +TCAAACTTGAAAATTATACTGAAATTCTCTGGTTTTGAATCTTTCTCAGTCTCCTTGATATCCCATTGGA +CGCTGATGTCGTTGATCAGTTCAACGTATTTGAAATCTTGTGCCCGGATGTACTCAGAGAAGTCAGGGTG +TTGTGACAGGACAATGTGCCAGAACTTATCTAGCGCTGAAGTGAATGTACTCCGCTTTTTGTAGATCTTC +TGAACCGCACGGAGGCGTGCTATTTCTTGGACATGTTAGTAATGACAATCAGTTGAAGGTTTGGTGACAC +CACATACCAGCAGCGCGCTCGGCTTGTTCCAGCTCTTCTTCAACGGAAGCAAGACCTATCAAAGCCTTTT +CTAGAGACTGTCTGTATCGCTGTTAGTAACAGAAGCCTAGTTGAGGAGCGTTTATTGACGCACAGTTCAC +TCATTGTAACTCTTGTTGAAAATTCTTGCTGATCTTCCTTTGTTTTTTTTTTTTTTTTTTTTTTCATTTA +GAAACCGTCATTTGAACCAGGTCCAAAGAGTTCAACACCAATTCGATAATGTCTACCGGGGGCCAAACAC +AGGTAAACAATGGAGAGTACTTCAAATCAATGAAACCACTACCTCTCGTTGATCCAACTCGCTCGCATGC +TCTCTTGATCCACTTCAATCCAATATCGAGCAGGATTGCAGCATCAAAAGATGACGGTACCCTCCGGATC +TGGTCCCTTGTTGGTTCACAACCCCCAGTTACGATAGATCCAGCTTCTAATAGAGCATTGCGCACGTCTG +CTAGTCGTATTCACGACTTCACATGGAAGCCTGATTCTGAGGCTGTCCTATACACTGTTGATGGTTCGCA +CTTAGTATCTGTTTGGAGTGCTAATCGTGGCTCACGAACTGCTACAATAGATACCAAAGTGAGCAACAGC +CATGTGGCCTGCTCCAAAGATGGCCTGTTTATTGCCTCATCGTCAAAACGTGTCGTTTGTATCATTGATA +AGTCCCATCATTTGGTGCAAAGCATTGATATGGGACCATCAGCAACTATATCTTGTATTTGTTGGGCGAA +TACCGCAGCGTTCTTTTTTGTGGGATTAACCAATGGTGATATTAGAGTATACCATCGCTCATCTCCCTCC +GATTGGGCGTTATTGCAAACCTTGACTGGTCATAATTCTCCAATTACAACCCTACAATTCTGCCCGCGTC +GTGATCTTGTTTCTGGATCTAGCCAGGGAATCATCTCATTTTGGTCATTGGATGAAAAGAATTTTTTAAC +GGTGACACAAACCATTGGTGATGTTGATGAACAAGTGGTGAATGTCTGTGTCAATAAGGACGCCACGACA +ATTGCAGTCTCCTATTCTTTGGGAAAGAGTGATGATGGAATCTGTAAGCTTTACAGCGTGGAAAGCTCAG +AGGTGGTTCATGAGATCAAGGCCAAAGCGTTGGAGTTTGTTTCGAATAAAACATCAAGTTTTGCTTGTGT +GGAGGATGGTAGAGTTATTCTTTATGAGATGGATAAGAATGATCAAGGAATGAGAAGAGATGTTAGAAAG +GAGGAGAGAAGAAAAGAGGAAAGAAGAGATGAAGTAAGACGTGACCCTCTTACGAGAAGAGATGATAGAA +AGGATGGCAGAAGAGACGATAGAAAGGATGAAAGAAGAGACGGGAGAAAAGACGATAGGAGGGATGACAG +AAGGGAGAGAGATGGAGAGAGAAGGGACGGCGAAAGAAGGGATGGCGAAAGAAGGGATGGAAAACGCGAT +GGTGGAAGGAGGTTTAGAAGGGATGGTAGAAGGTAAACCTTGTCTAAGTATCTTGTCGTCCGAATCCATG +TTGTAGACTAGCGATGCTTTATCCAACTTCCTGTTTGTTGCTACGACAACGGGAGTCTTTGGAGATCGGT +GAAGTTTTCTGCATAGAACCGAGGCCGAGGCCGAAGACCGAAGGGTTTAATGGGATAAAAAGGAACTGAA +ATTCTTCACCGCGGAAGTCTGATCCAATGCAATTATCAAATAATATGCTGCTTGAACTTCGCAAGGATAT +TAGAAATCTACTCTCATCAGCAGAGACGTCCCCTACTGATAGCACGATCCAGGACTTCCGTGGTCTTGAC +CTGAAGGATTCAGATGATGTAGATTCCCAAGATGGTGAGAATCTGGGGGAAGAAGCAGCACACTCCATAA +TTGGCGGCTCAAGTGTGATTGGTGGAAGTAATAGAGAGTCACCTGACCCTGAAGTGACAACAGATGATCT +CAAGAGGGGCCATTTTCTGAATCAGCACAAGATTTTCACTTTTAGTCTACCTTTTGGAGGCAATGGCAAC +CCTTTGTCACACTTGCCGTCACTGAGAACGTTTACGCAGGGCATCCCAATTTTCGGTAACAGTAGCACAG +ATGTCATAGATGATGAATTTAACGTGAGTTTATCGAAGGAGGAGATCCGCAACAAGTTGAAGAGACAAGA +AACCATCAACACTTTGGAGGAGGCTTATTTATTCCATGGTCTTAAAGGGCAGGGCTCATCTAGACTCAAA +GCAGTGACGAATGCGCTTACCCCAAACATCTCACTGCCGTCATTTGATGATAAGAAGAAGCATGAGTCCA +TATGGGAGACTATTGACGGTGATGTTGTGATCCTAGGTGGGTACAGAGGCTCCATCTTGAGGGAGACCAA +GTCTCATAAACGTGTGTGGATTCCTATAAGGGCTGGATTTAACATCAGAAAGATTGATCTTTTGATTGGA +CCTACAGATGAAGATGAGGAAAAAGCACAGAAGGAGATCTATTCAGATGATATCATGTCCCATGTTGGGC +CCATTGACATTTGCAGGAAGCTGATGTCGAAATTGAATAATGGGAAAACCAATGTCCATGCGTTTGGGTA +CGACTGGAGACTTTCTTCCGATATCTCTTCGCAAAAGCTGTTCGACTTTCTTCAAGAGTTGGAAAGAAAG +CGAGCGGAAAAGGGAATTGTGGGAAAGGGAACAATTGTGATTGCTCACTCAATGGGTGGTTTGGTGGCTC +ATCATGCTATGCAAAAGGATCCAAGTTTATTTAGAGGATTGGTGTATGCAGGAGTTCCTGCAGAATGTCC +CAATATTCTGGGTCCAATCAGAGTTGGGGACAGAGTCCTTGTGAATTCCAAGATCCTAACCGCAGAGGTG +AACTTCTTTATGAGATCTAGTTTTGTTTTCTTGCCACTTGAGGGAAGATGTTTTATGAACAGAAGGACAC +ATGAGCAATACACCTTGGACTACTTTGATGTGAACACATGGATCGAATACAACCTATCACCTCCTGTTTC +CAAAGACAGACTGGAAGGTGAGGCAAACCCCGCGAGCCCAATTACCGAATATCAGTTTTCTACATCATTT +GAGGAAGCCAAAACATATCTTGAGCACACACTAAAGAGGACCAAAAAATTCTTATTAGAGCTAGAATACG +ACCCAACGAAGGAATATCCACCACTGGCAATCGTTTATGGCAATGAGGTCCCTACAGTTCGTGGTGTGCG +GGTTGAGTCACCAGAAGGTATCAAACGCGGGGAGTATGATGACTTCTTCTATGGCCCTGGTGATGGTGTT +GTAAACCACAAGTGGTTGATGCCTGAGCGCAGAGGCTTCCCTGTTAATGCCAAAATTGCTAGTGACAAGG +GCCATATCTCGCTAATGTGCGACTTGGAGGCTATGGAACAAGCACTTGAGGCAATCATTTCTTGAAAACT +TCTAGCATTATAATGATGAATCAATTGTCATGACTACTCCTCAGCATCGTAGTATTTCACCTTTAGCCAC +AAGCTGCAGAAAGAGCACTGAAGAATAATCTCATAACCACCCATTCCATCCTCGGTACCATTCTCTTCTA +AATCAACCTCATTCAACACAAATCCCTCCTCGGATGTACACCGAGGGCAATCTTTCTTGAATATACAAAT +ATCACCATCTTCTTCACATTTGAAGTCATCTAATGTGAACACATCGAGACCTTCTCCAGTACTGAGTGTC +CCGTTCTTCTTGAATATCTCTGTCAGTTCTGCATCGTATTCATTTCGTAGCTTTTCGTTTGATAGTACCT +TGTAAGCCTCTTTGATCTGGGCGATCAGGTCGCCTTGTATGCTTTGGTTTGTCTTGTCTGGGTGAGTATT +TAGAAGTTTCGTGCGGTATGCTGACTTGATCTCTGCAAATGAAGCACACCTCTCAACAGAGAGTATTTCA +TAGTGCGATTTCCGTGCGATACTAGCCATCATAACGTTCGATGCTGTCATGAACCTGTTGCGTGAAAAGA +AAATAAATGTTAGAGAAAATGTCCGGTTTGGGACTTCTTCCGTTTTTGTAAAAATAGTGCCCTCGGATTA +TCTTTTATTAATTCCCGGTGGTGACTTCTCGCGACTTCAACGAAAATTTGCCGCTAACTCTCTCCAATCA +CAAACATCAACAGCATCAGCGTTAAAGTAGCACACGCATTGTAAAGTAAGAGTAGGACCGTACACTCATT +GCTTCAATCAAAGGCCACATAGCAGCTCAGTACATCCCCTCGAGAGCTATTTTTCTCCAAATTCGAAACA +AAAAAAAAAACAAAGTCGATATAGTGATAAAATGGATGCTTTTAATGCGGCCATTGAGGAGATTCGTGTC +TCCAAGAACGGTATATCTGGTAAGAAGATCAAGCATCTAACTGACATTGCCAGACAATTCCCTGCTCTCT +ATACGGAGTTCGTTCCTGCCGTATTAAACTACATCAGAAGTGCATCCGCTAGTCATAAACTGGGTGCCCT +GTATGTGCTGGACTCGATTGCTAGAGGTTTTCACGAGGACATTAAAGCCGGCAAGGACGCTACTGGGGAC +TTGAACAAGGCGATTTCTCTATTTGCTGCTAGTTTGAAGGAGGTCATTGAGATTGCTCATGAAAATGCGA +GTGAGGAACATAAGGAGAAGATTGTTAAGTTGCTCGAGATCTGGAGAAAGACTGGTACGTTTGATAATTC +TTTGATTTCTAGTGTTAGACTAGGCTCTACTACACCAACTGTCCCACCACCTTCTCTTGCTACCCCAGCA +GTCCCTACAGCTTCTCAAGCTAACGATTTGTTGAAACAGTTGGCTTCTATGGGTGGTGCCGCGCCACCAC +CACCTCCAGGAGCCAGCGCTCCAGGTTCTTCTGGCAGTAGTGTGCCATCGTTAGCACAAGCACCAGCATT +ACCAAATCTTAGTAACTTAGCGACAGCTGACCCAGCTCAACAACAAGCTGCTCTTTTCCAAATGTTACAA +GCCATGCAGTCGACTGGACAGATCCCTCCAATGCCACAGCCCCCATTGCAGCCTTCGTCCCAACAGCCGC +CGCAAGAGCTATCACAACAGGATCCTTCAACACAATCGCGCTGGGCCCGCGGGCCACAACCTCCTTCGCA +ACAGGAAGCATTCAACAGATTTAGACAACAGGATGGTGGTAGTCATGCAGCTGAAAGCTTCCGCGAAAGA +CAGTCAGGATTCTCACGTCGTGATCGTGACAGATCACCGCGTCGTCCAGTGAAAGATCGCGCTCCCCCTG +GCCATGTTATTGGTGAGGTCAACTTGCCAGGCACACCACACTTTCGTGAGCGTAATGTGTCATTTGACCA +ATCTCTTCCTGCTGGTTCTATTAAAGTTCTGAGTCGTACATTGTTTATTGGTGGTGTTCCTGCCTACATG +AACCAGGAAGAACTGGCTGCGATCTTGAGACCATATGCGGAGGTGCAGTCCGTTATTCTCAACGCTGAGC +GTAAACACGCCTTTGTGAAGGTGTACTCCAGAAAAGAGGCGGAGATGGTTGTCTCGTCTTTCAACAAGAA +TGGGCAATACAGTCTGCGTACCCGTTGGGGTGTTGGTTTCGGCCCTCGTGACTGTTGTGATTACCAACAT +GGTATGTCCATTATTCCTATTGCACGTTTGACGGATGCTGACAGACGTTGGATCAACGAGGCTCAATGGG +GTGGTATTGGGACAGAGCAACAACTGGTACCTGGCTTAATTATGGAAGAGCCAGATATCGAAATCGGTGA +AGGTGTCTCCTCAAAGGCTATTTCTCAAAAGATGCCTACAGATGGGTCCCGTAATGGTCCAAGATCTACT +AAACCTGGCGAGCCAGACGATATGTACACTGATCCTATTGGGGGTAACCAGCTGCCAAGCTTCATGCAGG +GTGGTAAGAGTGATGTGCTGTCTAACTTGTTCGGTGGAAACGGTGCTCCACCACAACAGGGGCCTCCTGC +TCCGGCACCTGTGCAGGCACCACCAGCACCAGCGGCTCAAACAAACGAGCAATTGAGCCAGTTGCTGTCG +TTCTTGAACCAGCAGCAAAAGTGATGAATGCAGAAGAGTTATTTGTTTTTTTACTTAGTATATAATCTGT +AATAATGATAAGGACCTTTGCTATTCTCAACCTTAGTAGGTGAAATCTTTAAATAAACATCCCCTTCCAA +TCACGTGACTATGCTGCGCCTACCGTAATTCCAAATTCTGACACTCCTGTATGATTTGGCAATAAGAAAG +TTATAAAAACATTATTGTTGAAGTGAGTTGTTTAGATCCAAAGCTGTTGATTGCAGCGAGAGACATGACA +TCCTTTGCAGATAGTTTTTGGACACCTGATTTCTCAACAGGTGAGTTATTTTCTACCCTTTAACCTTCCT +CAATCTTTAAATACTAACATCGTGTTCAGGTATTGAAGTGCTTTTTAACAAGTTGAATCAAGGTTGTGTT +GAGAATGACGAGTTCATCGATCTTTTCTCTGCCCGTATGCAACATGAGGAAGACTATGGCCGCAAATTGC +TGATTCTTCCTGACCTCCACTTGCCAAGCAAACACGGTTTTGACAAGGACGATGGGGCAAGCTTGAAGAA +CGCATTCAAAGGTGTGACAGAAGAGATGAGTAACGAGGGTCGCGTTCATCTGCAAATCGCACTGAATATC +AAAGAGCTCGTTGTTGATCCTTTTTCGAAGTGGTCGCAAGAGCACAAGCAAAGGGTGCAGTATTCTGAGA +GGATTCTAAAGGATAGTCTTAAAGTTTACAAGAACAAATACAAAGTTGTGGAAAAGGTGCAGAAGAAATA +CTTTAGCAAATGTCGAGTATATGAGACTCTGAAGCATGGACTGTCTGAAGAGGAAGTACTAAAAGAATTG +AGCATCAAAGATGAGAGTGATGGAGAGGAGAAATCAGTGTCAAATCAGGAGCAGCCACAAGTAGAGCAGG +AGTACGAAACGTTGGTAGTATTATGTGGTATTGAGTATGGAAAGATTGGTCTCAAGGATCTGTTGCACAA +GATGCTATCTGAGATACCTCAAACATCAATCAAGGTGCCAATCATTGGTGTCTACAACAACTGTTCCACT +GGATCGGCAATTGTCTCATGGATACAAGAGAATCTTGGAATTTCTAGCATCGACAAATGTGAGACTTTTG +GTAAGGATCTGATTGCCAACGGATACCTGAAGAAGGTCAATACCACTATGGGATTTGGTTCTAATTCAAC +GATGTTTGTTAATAGTGGTTCTTTTGCTTATCAATGGCGTGATGCCGCTTTTGAAATATCAGGATACAAC +AGAGAGAGGGCTAGCTCTATTGCCAGTACTAATGGGAACCCTTCAGAGTCATTAAGTAGCACCACAGCAT +CAATGACCAAATATCTTGATGATCTAAAGTCCCAATTCGAAGAGCCTACCTTGAGAAAAGCACATAAAGA +AATTGAAGAACTAGATGAGGCCTACCAATTAGAAGTGAAGAAACTGGACAAGATAAAATGTGACCTTGAA +GAGCTGATTCTTGATCATTTGTCGTTCATGGAAAAGTGTGAACTTGATCGTCTTAAAGCTCTGAAAAAGG +TCATTTTGGATTTCAGTGCTTCGATTTCCAACAACATCACTGCAATCAAATCCACCATTGATAAGATTTT +AGTGTATGAGGAATCCATCCACCCAGATAAGGATCTCTCATTTTTCCTCCAGAGCTACCGCACGGGATCA +TTCGCACCAAAAGTCGTTCTTTACGATAACTACTATGATAGTTTCAAAGATCAGATCTTTGGTGTGGATT +TGGAATTCCATTGCAAGAACGACCAAAAGACTGTGCCATTGATTGTATCATCGATTCTCTCATATATGGA +CAACATCTATCCGGAGTTGCCTAATGACGAGGTGAGAACCAAGATCTGGCTTATCCCAGTTAAACTTCAA +AGTACACATCAACTGAGAAGGGAGATTGAAAGTACCAAGACATTTAGTGATGAACTATTGTCGAAATATC +CTCCTGAAGTTGTTGCGAGTGTATTGAAGCTGTACCTCCTTGAGTTACCGGACTCCCTTGTGCCTAGCGA +AGTCTACGATTTGATCAAGTCTATCTATAATCAGTTTGGTTCTGATAGCGACGAGAAAAGTCGCATCAAT +GGTATTATCAATGTCCTAAAGGATCTGAGTAGACCGAATATTGCAACTTTGCAGGCACTGGCCACACATT +TCCAGAGACTAATTTCTATTTTGAAGGAGGGAAACAAGGACAATGACGCGATATGGAAGACATTCAGAAA +TGGGATCAGTCAAGAGTTTTCTAATTGCATTTTAAGACCAAAAAGTTCGAACAATATCAATATCAATGAT +ATGTTCACGTACAGATTCTTAAATGATTTGTTAACTAATAAGTCAGAAATCTTCAAGGCCTTA + diff --git a/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.lift.gff b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.lift.gff new file mode 100644 index 00000000..8dd53d7e --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.lift.gff @@ -0,0 +1,22 @@ +##gff-version 3 +##PAF BC_dic1 316 0 316 + PPLX03000005.1:95524-162156 66633 49670 50618 948 948 0 AS:i:1604 ms:i:1604 np:i:316 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:316M cs:Z::316 +PPLX03000005.1:95524-162156 miniprot mRNA 49671 50621 1604 + . ID=L000001;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_dic1 1 316 +PPLX03000005.1:95524-162156 miniprot CDS 49671 50621 1604 + 0 Parent=L000001;Rank=1;Identity=1.0000;Target=BC_dic1 1 316 +PPLX03000005.1:95524-162156 miniprot stop_codon 50619 50621 0 + 0 Parent=L000001;Rank=1 +##PAF BC_MATa2 269 0 269 + PPLX03000005.1:95524-162156 66633 41402 42265 804 807 0 AS:i:1363 ms:i:1392 np:i:269 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:96M56N173M cs:Z::96~gt56ag:127*aagR:45 +PPLX03000005.1:95524-162156 miniprot mRNA 41403 42268 1392 + . ID=L000002;Rank=1;Identity=0.9963;Positive=1.0000;Target=BC_MATa2 1 269 +PPLX03000005.1:95524-162156 miniprot CDS 41403 41690 477 + 0 Parent=L000002;Rank=1;Identity=1.0000;Target=BC_MATa2 1 96 +PPLX03000005.1:95524-162156 miniprot CDS 41747 42268 915 + 0 Parent=L000002;Rank=1;Identity=0.9942;Target=BC_MATa2 97 269 +PPLX03000005.1:95524-162156 miniprot stop_codon 42266 42268 0 + 0 Parent=L000002;Rank=1 +##PAF BC_MATa1 162 0 162 - PPLX03000005.1:95524-162156 66633 40292 40778 486 486 0 AS:i:812 ms:i:812 np:i:162 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:162M cs:Z::162 +PPLX03000005.1:95524-162156 miniprot mRNA 40290 40778 812 - . ID=L000003;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATa1 1 162 +PPLX03000005.1:95524-162156 miniprot CDS 40290 40778 812 - 0 Parent=L000003;Rank=1;Identity=1.0000;Target=BC_MATa1 1 162 +PPLX03000005.1:95524-162156 miniprot stop_codon 40290 40292 0 - 0 Parent=L000003;Rank=1 +##PAF BC_MATalpha1 233 0 233 - PPLX03000005.1:95524-162156 66633 36757 37456 699 699 0 AS:i:1232 ms:i:1232 np:i:233 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:233M cs:Z::233 +PPLX03000005.1:95524-162156 miniprot mRNA 36755 37456 1232 - . ID=L000004;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATalpha1 1 233 +PPLX03000005.1:95524-162156 miniprot CDS 36755 37456 1232 - 0 Parent=L000004;Rank=1;Identity=1.0000;Target=BC_MATalpha1 1 233 +PPLX03000005.1:95524-162156 miniprot stop_codon 36755 36757 0 - 0 Parent=L000004;Rank=1 +##PAF BC_sla2 1046 0 1046 + PPLX03000005.1:95524-162156 66633 24982 28120 3138 3138 0 AS:i:5179 ms:i:5179 np:i:1046 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:1046M cs:Z::1046 +PPLX03000005.1:95524-162156 miniprot mRNA 24983 28123 5179 + . ID=L000005;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_sla2 1 1046 +PPLX03000005.1:95524-162156 miniprot CDS 24983 28123 5179 + 0 Parent=L000005;Rank=1;Identity=1.0000;Target=BC_sla2 1 1046 +PPLX03000005.1:95524-162156 miniprot stop_codon 28121 28123 0 + 0 Parent=L000005;Rank=1 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.miniprot.gff b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.miniprot.gff new file mode 100644 index 00000000..feab2d39 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_PPLX03000005.1_95524-162156.miniprot.gff @@ -0,0 +1,84 @@ +##gff-version 3 +##PAF MH260578_SLA2 1072 29 1072 + PPLX03000005.1:95524-162156 66633 25003 28117 1719 3165 0 AS:i:2775 ms:i:2775 np:i:763 fs:i:0 st:i:0 da:i:21 do:i:3 cg:Z:94M2D73M1D99M1D18M7I14M2I256M5I54M4D136M1I29M1I11M1I183M4D59M cs:Z:*tctT:1*ctcI*cagS:2*cttI*cgtK:2*acgC*tctT*gctP*gatE*gagD:2*ccaS:18*ctcK*tcgD:2*aacE*gctT*ttaM:1*attV*ttgV:1*attL*cagK:1*gatT*gaaD*gttT:7*accL:9*caaP:3*gcaV*gaaG:1*tacF*aggK:1*ttgM*aacE:2*gatS*ggtN:1*gggD*agaT*tctL*ataR*caaT*ggtL:1-ggattc*aagR*ggcR*tatF*ggtQ*agaV:2*aaaP:3*accN:1*ttgI*atcL*agaQ:2*cagR:2*cggH:14*atcV:2*gtgR:2*caaS*aacD:1*gatN:3*gaaD:2*atgL:2*atgT:7*gatS*ctgF*cagG:1*cttD:4*attV-tca*catR*gatN*aagN*agaN:3*aaaI:6*cttI:1*actA:8*atcL*accI:5*attM:1*cgtK*tccM*acaS*ggaD:1*gatA*gatE*gctI:5*cagS*cgcK*tacF:1*tcgD:2*gctE:2*ttcY*gaaD:3*gatN:2*tccN:5*tctT:5*agaK:2*gagF*acaE:4*atgI*gttI*aagQ*gatN*gaaD*tcaE*tccD*gtcE*gaaN*gagV*gagN*actL:1-aga:1*gaaL*tctT*cgtE:1*acaK*aatT*ttgA*tcaA*gaaT*ccaT*attL*tctR*gagH*cagS:2*gtcP+FEQENER*tccQ:1*ttcQ:1*caaA:3*gcaT:4*gtcM+VR*gatN*tttA*tggF*agcV*aatE:2*tctQ*agtL*tttY:1*gctQ*gagQ:1*attQ*cgtQ:1*caaE:1*gagQ:1*gatQ:1*caaE*gctL*ctcQ:4*tatQ*gctQ*gaaL*caaA:1*agaQ*attQ*ttgY*ttcW:4*gtcR:2*caaA:1*cagA:1*cgtQ*attL:2*gagQ:3*cgcL*gagD:1*tacA:1*tcaR*caaH:11*ctgI:1*gctT:1*agaK:3*gagD*caaN:3*ctaM:2*caaS:3*cgtK:1*aagQ:3*actN:1*attL*acaA*gctS:3*tcaT:1*actQ*cagE:1*attL*ggcA:4*ttgQ*attL*caaN*aacL:1*catQ:2*ataV*actE*ctaY:16*cagT:6*aatT:1*ttcS:2*ctcI:11*gacE:6*aagR:1*ctgM:1*tcgA:2*gtgI:3*ggtD:2*cgtK:7*gacE:2*aaaR*gttF:1*ggtN:1*aaaT*ggtK*gatT*gagD*attL*gacQ*aagT:1*gagQ:1*caaE*attK*agaD*gatE:1*accE*ttgQ:2*gatA*gatT*gccL*gagD:1*aacA:2*tcgA:2*tccT*actA:2*gctN:2*aagN:2*atcL*gaaQ*gctE:1*gagQ:1*cagK+FKDVT:1*agaN*cagA*caaP:1*tcgD*gctE*ttcK*aatL*ccaK*gaaQ:2*caaE*ttaQ:1*aagQ*gatE:2*gaaL:1*ttaI:1*attM*ctcM:5*gatE*gcgT*ctgI*gtgK*gaaD:1*gccV:1*gctT:1*aagN*gagS*aatR:1*acaE*gctK*attS:1*gagM:1*atcN*gacK*caaL-gttttggttgat*cacN*ttaS:2*cttV*actL*tccG:1*gttI:4*ctcK:2*ctcI*aagN:1*ttaI:3*ttaV*tatF:10*cagL:6*ttaV:1*tccT*attL*atcL:2*gcgS:1*tccG*tctL*gctV*atgT:2*ggtA*gacS:3*gacN*ttcM*atcL*attV:2*ccaD:1*ggaA*gatN*cacE:1*gagL*attV:1*cgtD:1*ctcI*aatT*gacN:2*tctT:2*gctS:1*gttI:3*tctT:2*attL*gtgT:2*gttT:1*tcaQ:1*gcaD*gagF*gctQ:1*caaD*ttcL:1*gctD:2*agaK*gctD*actI:2*gaaI:2*gaaV*ttcY*ttcL*aaaE:2*cttY*tccH*gatS*agtN:1*gagV*ggcN*gaaK+S*gtgI*gaaD*gagD*aagQ:2*gtgI:1*atgI*gccN:2*ttgI*gcaD:3*aaaI:2*gatT:1*accL:2*actV:1*gacS*ttaM+K*gccT:1*agaS:1*aatK*cttI*ttgD*aacL:1*aacK:1+D*gcgG*aacE:1*ggcS:3*gaaD*actK:1*ttgM*cagE*aatH:1*gccS*gccR*tccV:1*tcaE*aagE:2*tccV*cagH:1*actS:3*tcgN*aaaQ*gatP*caaF:1*ccaV:1*atcM:1*caaS:2*tttI:1*attV:2*gctS:1*gttL*tccA:2*attS:1*gttI*accI*aatT*tccA:1*gaaK*cttY:2*aaaR:1*gctS:1*gctE:6*gcaN*aagN*aacG*aaaR:2*attH:3*gaaS*tacF:3*cacN:1*cgtK:8*gctS:2*gttI:1*cttY:2*aagN*attT:2*gaaR*actI:5*tctQ*gggN*aatK*aaaH:1*catN:1*cagE:1*attV:3*catR:6*gttA:3*gctS:1*tcgA:3*gctS*acgQ:1*ttgM:4*gacN*aatK:2*actD:3*aacK:1*acaN*tctL:2*agaK*agtQ:2*aatD:2*caaN*gacK*attL*ttaI*actI-cagagaagcaac:1*ggtK*agtH*ggtE*gagV*aatE:1*gttI:5*acaS*aatI:5*gtgA:15*atcS*gcaS:10*tatF*ggcS:1*agaK:1*gggD:2*agcD:2 +PPLX03000005.1:95524-162156 miniprot mRNA 25004 28117 2775 + . ID=BC000001;Rank=1;Identity=0.5431;Positive=0.7232;Target=MH260578_SLA2 30 1072 +PPLX03000005.1:95524-162156 miniprot CDS 25004 28117 2775 + 0 Parent=BC000001;Rank=1;Identity=0.5431;Target=MH260578_SLA2 30 1072 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 195 44 154 - PPLX03000005.1:95524-162156 66633 36946 37282 120 339 0 AS:i:161 ms:i:161 np:i:58 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaT*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaK:2*agtL*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS*agaS*aagR*agaH*gggR*cccV*tcaQ*agaK*aagE*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatR:2 +PPLX03000005.1:95524-162156 miniprot mRNA 36947 37282 161 - . ID=BC000002;Rank=1;Identity=0.3540;Positive=0.5133;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 45 154 +PPLX03000005.1:95524-162156 miniprot CDS 36947 37282 161 - 0 Parent=BC000002;Rank=1;Identity=0.3540;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 45 154 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATa1 181 122 181 - PPLX03000005.1:95524-162156 66633 40292 40469 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +PPLX03000005.1:95524-162156 miniprot mRNA 40290 40469 171 - . ID=BC000003;Rank=1;Identity=0.5593;Positive=0.7458;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +PPLX03000005.1:95524-162156 miniprot CDS 40290 40469 171 - 0 Parent=BC000003;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +PPLX03000005.1:95524-162156 miniprot stop_codon 40290 40292 0 - 0 Parent=BC000003;Rank=1 +##PAF 5476_sc5314_MTL_A_MTLA1 210 116 197 - PPLX03000005.1:95524-162156 66633 40301 40520 105 243 0 AS:i:169 ms:i:158 np:i:49 fs:i:0 st:i:0 da:i:24 do:i:9 cg:Z:14M7I1M1I58M cs:Z::2*gaaD*gcaQ*gttM*atgD*gccD*tcaD*ccaK:2*aatE*aacD*aatD+ILIKGEE:1+K*caaK*caaS:2*cgaK:1*agaQ*catR*attL*tccD*aagN*gaaS*gctT*cgcK*agcE*cttF:3*gcaV:2*actK*actN:1*aatQ:1*accN:1*aagR:2*gcaE*ataL:4*tccH:1*atgV*acaS*gcaL*cttS:1*gtaI:10*accK:1 +PPLX03000005.1:95524-162156 miniprot mRNA 40302 40520 158 - . ID=BC000004;Rank=1;Identity=0.4321;Positive=0.6049;Target=5476_sc5314_MTL_A_MTLA1 117 197 +PPLX03000005.1:95524-162156 miniprot CDS 40302 40520 158 - 0 Parent=BC000004;Rank=1;Identity=0.4321;Target=5476_sc5314_MTL_A_MTLA1 117 197 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1019 7 1019 + PPLX03000005.1:95524-162156 66633 24997 28108 1533 3135 0 AS:i:2456 ms:i:2448 np:i:714 fs:i:0 st:i:0 da:i:15 do:i:12 cg:Z:259M1I6M3I15M7D7M4D5M2I254M6D35M4D11M7D70M1I34M1D25M1I227M4D56M cs:Z::1*gagN*tctL:1*ctcI:1*actA*gccS:1*cgtK:2*acgC*tctT:2*gagA*actA:20*ctcR*tcgE:2*aacH*gctC:2*attL:4*aatS*gatN*gaaD*gttT:1*ttaI:2*gctT:2*accV:9*caaP*tctT*gctC:1*gcaI*gaaG:2*aggK:1*ttgI*aacS:1*atcL*gatE*ggtS:1*gggS:1*tctF*ataS*caaN*ggtN*gatG*ggaT*ttcA*aagA:2*ggtT:7*accF:2*atcE*agaQ:2*cagK:2*cggH*aatD:6*accM:6*atcV:2*gtgR:2*caaS*aacD:1*gatN:2*tatF:1*gccS:4*atgL:7*gatN:3*cttV:2*gcaS*acaS:1*tcaR:1*gatT*aagS*agaE:3*aaaV:2*gcaS:3*cttI*gttI*actA:8*atcL*accI:3*aggK:1*attL:1*cgtK:1*acaS*ggaE:2*gatE*gctV*ttaI*gagA:3*cagD:1*tacF*gagD*tcgV:2*gctR:12*cgcK:3*tctT:1*atcV:6*gagS*acaS*gctP:1*aacI:1*atgQ+D*gttE*aagE:1*gaaP*tcaQ*tccG+FPL:1*gaaR:1*gagR*actS*ccaS*agaV*ccaS*gaaN:1*cgtE*cagE*acaS:1*ttgI-tcagaaccaatttctgagcag:1*actR*gtcS:1*cagN*ttcI:1-caaccaattgct*gcaS:4+AM:2*tttS*tggF*agcV*aatR:2*tctQ*agtE*tttL:1*gctE*gagQ:1*attR*cgtQ*ttgA*caaE*caaE*gagR:1*gatQ:1*caaE*gctL:1*caaE:2*cagM*tatL*gctQ*gaaR*caaD:1*agaA*attR*ttgF*ttcW:1*cagE:2*gtcR:2*caaA*gaaV*cagS:1*cgtE*attL:2*gagS:3*cgcA*gagD:1*tacM:1*tcaR:4*cgcK*gtaL:1*gagQ:2*cgcQ:3*gctT:1*agaG:2*tatH:1*caaR:3*ctaM:1*cagE:7*gccV:2*actN:1*attL*acaR*gctI*accV:2*tcaT:1*actE*cagE:1*attL*ggcA*tccV:1*gatT*gagS*ttgQ*attC*caaS*aacH:1*catE:2*ataV*actQ*ctaY:23*aatG:3*aaaI*ctcV:9*gccS:12*tcgA:2*gtgI:3*ggtD:2*cgtK:9*gagA*aaaR*gttI:2*ggtN*aaaD*ggtS*gatE*gagQ*attV*gacQ*aagM:1*gagR*ttgT:1*attL:2*ctcM*accE*ttgT:2*gatK*gatS*gccL:1*agaE*aacT*caaH:1*tcgQ:2*tccA*actS*attL:1*gctS:2*aagS:4*gctE*ttgI-gagaaccagttaagacag*caaK*tctA:2*ttcS*aatE*ccaL*gaaK*ttgE*gaaG*caaG:1*ctaN*aagE*gatE*aagA*gatQ*gaaQ:1*ttaV*gaaQ*attM:3*acaA:3*gcgT*ctgM*gtgQ:3-aaagctcagaag:1*aatQ*gaaH*acaQ:3*gagK*cagE*atcA*gacE-caagttttggttgatcactta:1*aagR*cttV*actL*tccR:1*gttI:1*gctT*atcL:1*ctcK*agtV*ggcS*ctcS*aagD:2*caaR*gacE*gccF*ttaV*tatF*gaaN:1*gacE:2*atgV*caaY*gctS*ggtN*aacT*cagD*aacI*tcaP*tcgT*cctA:3*ctcG*tccT*attM*atcL:1*aaaR*gcgV:1*tccH*tctL*gctS*atgT*gacQ:1*ggtS*gacT*tctT*tttM*aatT:1*ttcY:1*attA:1*gggN*ccaM*aatK:1*gatE+S*cacE*gctS*gagG*attL:1*cgtK*tctA*ctcI*aatI*gacE*ttcL:1*tctD:2*gctG*gacN*gttL*ttaF*ttaS:1*tctV:1*ggtA*attV*gtgL:1*cttD*gttR:1*tcaD:1*gcaV*gagQ-gct*gatE*caaI*ttcL*ataV*gctE*actG*gcgS*agaK*gctD*actL:1*gagH*gaaM*tcgV:1*gaaL:2*aaaE:1*ttgM:2*gatN*agtH+L:1*gagK*ggcL:1*gtgI*gaaH:1*aagR:2*gtgR:1*atgI*gccT:2*ttgI*gcaN:3*aaaV*cttI:1*gatS:1*accM:1*ttgV*actV:1*gacV*ttaF*gccR*ccaD*agaP*tctG*aatR*cttK*ttgT*aacP*agcV*aacS*gttS*gcgD*aacE:2*gacY*cttF*gtgL*gaaD*actK:2*cagA:1*gcgT*gccQ:1*tccA*attV*tcaE:2*tcaA*tccG*cagL:1*actE:1*ctaM*ctcS*tcgT*aaaR*gatH*caaK*gacL*ccaE*tctV*atcQ*tcaE*caaD*atcG*gacS*tttL:1*attV:1*aaaE:2*gttI:1*gctC:1*attS:1*gttI*accI:1*tccA*attV*gaaK:2*attL*aaaE:1*gctC:1*gctD*tcaA:5*gcaS*aagR*aacG*aaaR:2*attM:1*aagR*actA*gaaL*tacF:3*cacN:10*gctS:1*tctQ:2*cttY*tccA:1*aagG:3*gaaR*actM:5*tctA:1*aatT*aaaN:1*catS:1*cagE:5*catN:3*gctS*tcaA:1*gttA:3*gctS*gctS:4*gctS*acgD*tttL*ttgM:1*aagQ*actS*caaH*gacL:3*actE:2*aagR*aacK:3*gccS:1*agaK*agtM:2*aatT*caaK:1*caaR*gacS*attL:1*actL-cagagaagcaac*ggcS*ggtD*agtE*ggtS*gagN*aatK*gatT:4*aaaQ:2*aatA:3*aaaR:1*gtgA:1*atgL:10*aatS:2*atcS*gcaH:10*tatF*ggcS:3*gggE*gacE:1 +PPLX03000005.1:95524-162156 miniprot mRNA 24998 28108 2448 + . ID=BC000005;Rank=1;Identity=0.4890;Positive=0.6833;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +PPLX03000005.1:95524-162156 miniprot CDS 24998 28108 2448 + 0 Parent=BC000005;Rank=1;Identity=0.4890;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 187 128 187 - PPLX03000005.1:95524-162156 66633 40292 40469 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +PPLX03000005.1:95524-162156 miniprot mRNA 40290 40469 171 - . ID=BC000006;Rank=1;Identity=0.5593;Positive=0.7458;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +PPLX03000005.1:95524-162156 miniprot CDS 40290 40469 171 - 0 Parent=BC000006;Rank=1;Identity=0.5593;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +PPLX03000005.1:95524-162156 miniprot stop_codon 40290 40292 0 - 0 Parent=BC000006;Rank=1 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 190 41 151 - PPLX03000005.1:95524-162156 66633 36946 37282 126 339 0 AS:i:172 ms:i:172 np:i:59 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaS*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaN:3*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS:1*aagR*agaH*gggR*cccV*tcaQ*agaK*aagQ*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatI:2 +PPLX03000005.1:95524-162156 miniprot mRNA 36947 37282 172 - . ID=BC000007;Rank=1;Identity=0.3717;Positive=0.5221;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 42 151 +PPLX03000005.1:95524-162156 miniprot CDS 36947 37282 172 - 0 Parent=BC000007;Rank=1;Identity=0.3717;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 42 151 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 294 0 294 + PPLX03000005.1:95524-162156 66633 49742 50618 504 882 0 AS:i:856 ms:i:856 np:i:214 fs:i:0 st:i:0 da:i:72 do:i:0 cg:Z:98M1I99M1I95M cs:Z:*gccM:1*gctS*aaaD*accK*ataS:1*gccK*accS*aagI*accA:1*accS*accA*aagN*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctK*ttaV:1*aagG*ccaD:2*tttV*cagS*atgL:2*tcgK:1*ctgI:1*gcaT*gatE:1*attV*acaL*ggtA*atcA:1*tctA:2*tctT:1*ggcS:8*ctgT*accA:3*gccV:2*tacK:3*agtI*ttcM*gttT+D:1*gccT*gatR*aagG*atgQ*agtA:1*atgT*gctF*tatQ:2*cccA*atcA:2*gttI*agtA:5*atcV:4*tctA:1*attV:8*actN*ggtS:2*atcE*gaaS:3*aacH:2*aatH:1*tttL:2*gtgL*gccL*cgaK:1*gtgT*actR:2*ggtN*cctL*agtT:6*tctG*acaP:2*gtgA:2*gttI:3*tcaA:4*actS:2*ttgV:2*gaaK*atgL:1*atcV+E*tccN*ttcL*ggaS:1*aaaD*aagP*gagK*tccT*ttcK*ttcA:1*tttH:2*tctA:2*cttI:3*gccV:3*attV:13*gcaS:3*aacG*catQ:1*gtgS*gtgF*cacG:2*actK*aaaD:1*ttgI*aagS*gacR:2*cccL*agcG:6*gtgT:1*agtA:1*gtgI:4*aatH:2*atcL*attT:1*ctcI*gttA*gtaL:3*aagR:1*atgL*agaK:3 +PPLX03000005.1:95524-162156 miniprot mRNA 49743 50621 856 + . ID=BC000008;Rank=1;Identity=0.5714;Positive=0.7279;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +PPLX03000005.1:95524-162156 miniprot CDS 49743 50621 856 + 0 Parent=BC000008;Rank=1;Identity=0.5714;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +PPLX03000005.1:95524-162156 miniprot stop_codon 50619 50621 0 + 0 Parent=BC000008;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 305 7 305 + PPLX03000005.1:95524-162156 66633 49739 50618 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gacK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +PPLX03000005.1:95524-162156 miniprot mRNA 49740 50621 818 + . ID=BC000009;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 8 305 +PPLX03000005.1:95524-162156 miniprot CDS 49740 50621 818 + 0 Parent=BC000009;Rank=1;Identity=0.5537;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 8 305 +PPLX03000005.1:95524-162156 miniprot stop_codon 50619 50621 0 + 0 Parent=BC000009;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1045 0 1045 + PPLX03000005.1:95524-162156 66633 24988 28108 1827 3165 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D54M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gagS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE*agtE +PPLX03000005.1:95524-162156 miniprot mRNA 24989 28108 2946 + . ID=BC000010;Rank=1;Identity=0.5773;Positive=0.7488;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +PPLX03000005.1:95524-162156 miniprot CDS 24989 28108 2946 + 0 Parent=BC000010;Rank=1;Identity=0.5773;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1044 0 1044 + PPLX03000005.1:95524-162156 66633 24988 28105 1827 3162 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:15 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D53M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gagS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE +PPLX03000005.1:95524-162156 miniprot mRNA 24989 28105 2946 + . ID=BC000011;Rank=1;Identity=0.5778;Positive=0.7495;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +PPLX03000005.1:95524-162156 miniprot CDS 24989 28105 2946 + 0 Parent=BC000011;Rank=1;Identity=0.5778;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 305 7 305 + PPLX03000005.1:95524-162156 66633 49739 50618 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gacK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +PPLX03000005.1:95524-162156 miniprot mRNA 49740 50621 818 + . ID=BC000012;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 8 305 +PPLX03000005.1:95524-162156 miniprot CDS 49740 50621 818 + 0 Parent=BC000012;Rank=1;Identity=0.5537;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 8 305 +PPLX03000005.1:95524-162156 miniprot stop_codon 50619 50621 0 + 0 Parent=BC000012;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 305 7 305 + PPLX03000005.1:95524-162156 66633 49739 50618 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gacK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +PPLX03000005.1:95524-162156 miniprot mRNA 49740 50621 818 + . ID=BC000013;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 8 305 +PPLX03000005.1:95524-162156 miniprot CDS 49740 50621 818 + 0 Parent=BC000013;Rank=1;Identity=0.5537;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 8 305 +PPLX03000005.1:95524-162156 miniprot stop_codon 50619 50621 0 + 0 Parent=BC000013;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1044 0 1044 + PPLX03000005.1:95524-162156 66633 24988 28105 1827 3162 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:15 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D53M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gagS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE +PPLX03000005.1:95524-162156 miniprot mRNA 24989 28105 2946 + . ID=BC000014;Rank=1;Identity=0.5778;Positive=0.7495;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +PPLX03000005.1:95524-162156 miniprot CDS 24989 28105 2946 + 0 Parent=BC000014;Rank=1;Identity=0.5778;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 287 14 287 + PPLX03000005.1:95524-162156 66633 49805 50618 477 819 0 AS:i:799 ms:i:799 np:i:201 fs:i:0 st:i:0 da:i:135 do:i:0 cg:Z:77M1I99M1I95M cs:Z::1*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctK*ttaV:1*aagG*ccaD:2*tttV*cagS*atgL:1*ttcY*tcgK:1*ctgV:1*gcaT*gatE:1*attV*acaL*ggtA*atcA:1*tctA:4*ggcS:8*ctgT*accA:3*gccV:2*tacK:2*gagG*agtI*ttcM*gttT+D:1*gccT*gatK*aagG*atgQ*agtA:1*atgT*gctF*tatQ:2*cccA*atcA:2*gttI*agtA:5*atcV:4*tctA:1*attV:8*actN*ggtS:2*atcE*gaaS:3*aacH:2*aatH:1*tttL:2*gtgL*gccL*cgaK:1*gtgT*actR:2*ggtN*cctI*agtT:6*tctG*acaP:2*gtgA:2*gttI:3*tcaA:4*actS:2*ttgV:2*gaaK*atgL:1*atcV+E*tccN*ttcL*ggaS:1*aaaD*aagP*gagK*tccT*ttcK*ttcA:1*tttH:2*tctA:2*cttI:3*gccV:3*attV:13*gcaS:3*aacG*catQ:1*gtgS*gtgF*cacG:2*actK*aaaD:1*ttgI*aagS*gacR:2*cccL*agcG:6*gtgT:1*agtA:1*gtgI:4*aatH:2*atcL*attT:1*ctcI*gttA*gtaL:3*aagR:1*atgL*agaK:3 +PPLX03000005.1:95524-162156 miniprot mRNA 49806 50621 799 + . ID=BC000015;Rank=1;Identity=0.5824;Positive=0.7363;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +PPLX03000005.1:95524-162156 miniprot CDS 49806 50621 799 + 0 Parent=BC000015;Rank=1;Identity=0.5824;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +PPLX03000005.1:95524-162156 miniprot stop_codon 50619 50621 0 + 0 Parent=BC000015;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 195 44 154 - PPLX03000005.1:95524-162156 66633 36946 37282 120 339 0 AS:i:161 ms:i:161 np:i:58 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaT*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaK:2*agtL*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS*agaS*aagR*agaH*gggR*cccV*tcaQ*agaK*aagE*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatR:2 +PPLX03000005.1:95524-162156 miniprot mRNA 36947 37282 161 - . ID=BC000016;Rank=1;Identity=0.3540;Positive=0.5133;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 45 154 +PPLX03000005.1:95524-162156 miniprot CDS 36947 37282 161 - 0 Parent=BC000016;Rank=1;Identity=0.3540;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 45 154 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 181 122 181 - PPLX03000005.1:95524-162156 66633 40292 40469 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +PPLX03000005.1:95524-162156 miniprot mRNA 40290 40469 171 - . ID=BC000017;Rank=1;Identity=0.5593;Positive=0.7458;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +PPLX03000005.1:95524-162156 miniprot CDS 40290 40469 171 - 0 Parent=BC000017;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +PPLX03000005.1:95524-162156 miniprot stop_codon 40290 40292 0 - 0 Parent=BC000017;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 440 0 440 + PPLX03000005.1:95524-162156 66633 26746 28108 591 1371 0 AS:i:863 ms:i:863 np:i:295 fs:i:0 st:i:0 da:i:975 do:i:12 cg:Z:15M4D11M7D70M1I34M1D25M1I224M1I7M5D51M cs:Z:*gaaQ*attM:3*acaA:3*gcgT*ctgM*gtgQ:3-aaagctcagaag:1*aatQ*gaaH*acaQ:3*gagK*cagE*atcA*gacE-caagttttggttgatcactta:1*aagR*cttV*actL*tccR:1*gttI:1*gctT*atcL:1*ctcK*agtV*ggcS*ctcT*aagD:2*caaR*gacE*gccF*ttaV*tatF*gaaN:1*gacE:2*atgV*caaY*gctS*ggtN*aacT*cagD*aacI*tcaP*tcgT*cctA:3*ctcG*tccT*attM*atcL:2*gcgV:1*tccH*tctL*gctS*atgT*gacQ:1*ggtS*gacT*tctT*tttM*aatT:1*ttcY:1*attA:1*gggN*ccaM*aatR:2+S*cacE*gctS*gagG*attL:1*cgtK*tctA*ctcI*aatT*gacE:2*tctD:2*gctG*gacN*gttL*ttaF*ttaS:1*tctV:1*ggtA*attV*gtgL:1*cttD*gttR:1*tcaD:1*gcaV*gagQ-gct*gatE*caaI*ttcL*ataV*gctE*actG:1*agaK*gctD*actL:1*gagH*gaaM*tcgV:1*gaaL:2*aaaE:1*ttgM:3*agtH+L:1*gagK*ggcL:1*gtgI*gaaH:1*aagR:2*gtgR:1*atgI*gccT:2*ttgI*gcaN:3*aaaV*cttI:1*gatS:1*accM:1*ttgV*actV:1*gacV*ttaF*gccR*ccaD*agaP*tctG*aatR*cttK*ttgS*aacP*agcV*aacS*gttS*gcgD*aacE:2*gacY*cttF*gtgL*gaaD*actK:2*cagA:1*gcgT*gccQ:1*tccA*attV*tcaE:2*tcaA*tccD*cagL:1*actE:1*ctaM*ctcS*tcgT*aaaR*gatH*caaK*gacL*ccaE*tctV*atcQ*tcaE*caaG*atcG*gacS*tttL:1*attV:1*aaaE:2*gttI:1*gctC:1*attS:1*gttI*accI:1*tccA*attV*gaaK:2*attL*aaaE:1*gctC:1*gctE*tcaA:5*gcaS*aagR*aacG:3*attL:1*aagR*actA*gaaL*tacF:3*cacN:10*gctS:1*tctQ:2*cttY*tccA:1*aagG:3*gaaR*actM:5*tctA:1*aatT*aaaN:1*catS:1*cagE:5*catN:3*gctS*tcaA:1*gttA:3*gctS*gctS:4*gctS*acgD*tttL*ttgM:1*aagQ*actS*caaH*gacL:3*actE:2*aagR*aacK:3*gccS:1*agaK*agtM:2*aatT*caaK:1*caaR*gacS+L*attL:2*cagD*agaE:2-ggcggtagtggtgag*aatK*gatT:4*aaaQ:2*aatA:3*aaaR:1*gtgA:1*atgL:10*aatS:2*atcS*gcaQ:10*tatF*ggcS:3*gggE*gacE:1 +PPLX03000005.1:95524-162156 miniprot mRNA 26747 28108 863 + . ID=BC000018;Rank=1;Identity=0.4311;Positive=0.6455;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +PPLX03000005.1:95524-162156 miniprot CDS 26747 28108 863 + 0 Parent=BC000018;Rank=1;Identity=0.4311;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 671 0 671 + PPLX03000005.1:95524-162156 66633 24988 28117 825 2121 0 AS:i:1065 ms:i:1065 np:i:398 fs:i:0 st:i:0 da:i:6 do:i:3 cg:Z:94M2I6M10I3M2I75M7I2M1I86M34I14M1I9M9I9M1I24M6I19M3D8M1I21M2I39M22D45M7D12M1D25M1I15M1I13M2D12M1I5M2I21M1D4M1260N7M3I19M cs:Z:*aagM*acgS*atcR*tctE:1*tctQ*gatT:1*cagE*actK*gccS*cttI*cgtK:2*acgL:1*gctG*gatG*gagD*actV:20*ctcK*tcgT:1*tggF:1*gctL:2*attL*ttgQ:1*attY*cagA*aatT:2*gttL:6*atcV*accL*atcL:8*caaP:3*gcaV:2*tacI:2*ttgI*aacD:2*gatE*ggtS:1*gggS:1+IF:1*ataV*caaD*ggtS:1*ggaD+GSKYGSSRMR*ttcY*aagI:1+DE:1*ggtA*agaK*ttgA:1*aaaG:3*accF*tacF:1*atcV*agaE:5*cggK*aatS:1*cgtK:4*accI:6*atcV:3*actS:1*caaS*aacD:1*gatN:4*gccT*atcV*atgL:3*tccN:1*caaE:1*tcaV*cttA:1*gatN:2*agaK:4*acaG:2*catN*gatR:1+SGFGSFS*agaS*tctG+L*gaaD:1*aaaR*attG*tcaA*gcaV:1*gtcI:3*actS:1*tctT:3*tatF:2*atcV:3*ttgM*aggK:1*attL:1*cgtK*tccQ*acaM:1*tctD*gatY:3*gagS*ccaG:1*aagF*cagD:2*gagT*tcgE:1*catC:3*ttcN:3*gctF:2*tccN*tccT*attV*cgcQ*tacP:1*actK*tctN:5*agaT:2*gagS*acaD*gctP*cctM*aacS*ctcR*atgI*gttI*aagD:2+DDAEVNDVVFEKRSKSSVSSLPSRTPTRENLRDP:1*tccP:1*gaaH:1*gagA*actP:1*agaV*ccaA*gaaR*tctP*cgtA*cagE+V*acaR*aatS:2*gaaQ:1*attA*tctA*gagP+VVPIATGVV*cagM:1*actV:1*tccT*cagG*ttcY*cagS:1+P:1*attL*gctQ*gcaV*caaV:3*gtcA*gatE:3*aatI*cagP*caaS*tctT:1*tttA*gagP*gctT*gagR*caaS*attV+VASHDD*cgtL:1*caaI*caaE*gagQ*cgtQ*gatK*gcaQ:1*gctQ*ctcI:6*gaaL:1-caaagaatt:1*ttcE*gaaH:1*cagK:1*gtcL:1+L:5*cgtA*attL*gcaL:1*gagQ*caaR*ttgI*cttQ*cgcQ*gagQ*cagE*tacM*cagE*tcaK*caaE:1+LI:1*ggcQ*cgcK*gtaT:1*gagS:1*gagQ*cgcN:1*ctgI*ctgM*gctS*ttgF*agaK*ggaN:3*caaR:2*cttV*ctaM:1*cagE:3*cagK:2*aagQ*gccK:2*actS:1*attL-acagctaccactcaatcagctactcagcaaattggctccaaggatgagttgattcaaaacttgcat:1*caaS*ataV*actK*ctaE*tggF:1*aacE:2*gagQ:15*ttgI:1*aatK:1*ttcM:2*ctcT:1*caaD*aaaD*gccL:1*tctE:1*caaE*gaaS*gccE*attV-gacaagcgtgagaaattggaa:1*gacK:3*aagS*aacA*gtgL*gaaS*ttgN*gctV:1-cta:2*gaaN*cgtN*gacN*cgtK*gctG*cgtE*ttgS*gacE*ttgR:1*aaaE*gttA:1*ggtD*ggtR*aaaS:1*gatM*gagI*attL*gacK*aagS:1+V:1*ttgS*caaS:1*agaK*gatS:1*accQ*ttgD*aaaP*ttaI*gatA*gatS*gccL*gagD+V*agaS*aacD*caaL*tcaA*tcgT*aacD:2*actN*attA:1*gctI*caaD-cacaag*aaaG*gagD:1*gaaN*gctT*ttgI*gagV:1*cagI:1*agaP*cagE+F*caaV:1*tcgL*gctL*ttcL+RC*aatP*ccaQ:1*ttgM*gaaQ*caaT*ttaI*ctaA*aagE:1*aagF*gatL*gaaR*gagC:1*gaaS*attS*ctcA*caaK:2-atg:1*gatS*gcgV*ctgI~gt1260ag:2*ctaN:2*gcaF*agaQ+EKL:7*aagQ:1*ggcS:2*gacY:3*agcD:1*gaaV +PPLX03000005.1:95524-162156 miniprot mRNA 24989 28117 1065 + . ID=BC000019;Rank=1;Identity=0.3890;Positive=0.5629;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 671 +PPLX03000005.1:95524-162156 miniprot CDS 24989 26779 995 + 0 Parent=BC000019;Rank=1;Identity=0.3791;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 642 +PPLX03000005.1:95524-162156 miniprot CDS 28040 28117 70 + 0 Parent=BC000019;Rank=1;Identity=0.6207;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 643 671 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 228 111 211 - PPLX03000005.1:95524-162156 66633 40301 40598 117 306 0 AS:i:157 ms:i:157 np:i:56 fs:i:0 st:i:0 da:i:180 do:i:9 cg:Z:29M2D12M3I56M cs:Z::1*aacD*tgtV*tcaK:1*ataE:2*tctM:1*catF*tctD*cctT*ccgE*gaaH*gcaE*tcaC*tctT*atgD*aatT*actS*tctE*aatD*gtcI*tgtS*tctD*gttK:2-gcagtt*atgI*gccS:1*ccaT*gacN:1*aatD:1*aatP:2*caaN+CYP*aagT*aagY*cgaK:1*agaS*catF:1*tccK*aagH:1*gctS:1*agcG*cttI:3*gcaI:1*gaaK*actV*actK*aaaQ*aatC:1*accN*agaT*aagS:2*gcaL*ataY:2*gagQ:1*tccL*ggtD*atgL:1*gcaP*cttS:3*gtgI:8*accA:1 +PPLX03000005.1:95524-162156 miniprot mRNA 40302 40598 157 - . ID=BC000020;Rank=1;Identity=0.3824;Positive=0.5490;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 112 211 +PPLX03000005.1:95524-162156 miniprot CDS 40302 40598 157 - 0 Parent=BC000020;Rank=1;Identity=0.3824;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 112 211 +##PAF 4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 212 51 212 - PPLX03000005.1:95524-162156 66633 34522 37282 153 507 0 AS:i:137 ms:i:166 np:i:82 fs:i:0 st:i:0 da:i:174 do:i:0 cg:Z:14M4I19M3I31M1I21M1D18M2D8M2295N4M6I11M5D21M cs:Z::1*tccE:2*aagS*ataL:1*aagT*cttP:1*gccI:1*tatL*gaaQ+EILI:2*attN*gaaQ*ggaA*ctaT*aagR*gatN*catC*aaaA*gctG*ggtD*caaS*gatS*agtW*agtG*acgS*tacQ*atcL+FDM*cgcK*caaV*catP*ttgT:1*agaK*aagS:1*gggI*cccR*tcaC*agaK:1*cggT*acaR*aagC:6*tatF:3*ttcY:2*aacS*gttI+Y*gcaN*acaV*gatE*caaH:1*ggaR*gtcE:2*tctT*caaL:1*gcgG:1*gaaL:1*gagK*aggN*aatE:1*gaaN-atc*caaK*cttA*gtaI:1*aagN:2*tccA*acaI:3*ttgS*tctQ*gttA*ttgI*agtN*gatQ-cgcctt*cccD:1*ttcV:3*ttgF*cttK~gt2295ag*gtaA:1*caaG*ataL+EVETFP*ttaI:1*ttcY*tctT*aagS:1*attV*agtR:1*cgtE:1-cttcgccaactatat*ctcW*tccT*tcgF:2*acaR*gtcN*aaaN*ttcE:1*attA*cttV:1*tccD*agcI*tccY*tttY:3*ctgV +PPLX03000005.1:95524-162156 miniprot mRNA 34520 37282 166 - . ID=BC000021;Rank=1;Identity=0.3018;Positive=0.4852;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 52 212 +PPLX03000005.1:95524-162156 miniprot CDS 36941 37282 141 - 0 Parent=BC000021;Rank=1;Identity=0.3197;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 52 170 +PPLX03000005.1:95524-162156 miniprot CDS 34520 34645 25 - 0 Parent=BC000021;Rank=1;Identity=0.2553;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 171 212 +PPLX03000005.1:95524-162156 miniprot stop_codon 34520 34522 0 - 0 Parent=BC000021;Rank=1 +##PAF 1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1064 0 1064 + PPLX03000005.1:95524-162156 66633 24979 28114 1773 3210 0 AS:i:2844 ms:i:2844 np:i:773 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:101M1D163M2D41M1D6M12I259M9I24M3I159M1I230M2D56M cs:Z:*gtgM*atgA*tccT*aagL*acgS*atcK*tctA:1*tctA*gatE:1*cagT*actV*gccH*cttI*cgtK:3*tctN*gctP*gatE:10*gcgQ:2*gttI:4*cacN*aagH:1*tcaG*ctcR*tcgA:3*gctG*ttaM:2*ttgQ:1*attL:6*ttaV:15*caaP:1*gctV:1*gcaR:1*ggtA*tacQ*aggQ:1*ttgR*aacS:1*atcL*gatE*ggtS:1*gggA:1*tctG*ataA*caaH*ggtA-gat:1*ttcR*aagH*ggcT:1*ggtA*agaK:2*aaaY:3*accG*tacL:1*atcL*agaK:2*cagN:2*cggK:2*cgtP*ggtD:13*gtgR*actN*gtcI*caaD*aacD:1*gatN:3*gaaQ:2*atgV:3*tccD:3*tcaA*cttI:1*gatN*ctgF:4*ttcI:1*acaS*attL*tcaK:1*gatG*aagR*agaP*tctN:2*aaaR:4*gtcI:3*actA:6*aagR:1*atcT:1*tcgT:4*attM*tacH*cgtA*tccT:1*ggaN*tctD:7*aagR*cagS:2*gagY:3*gctQ:2*ttcL:3*gctY:4*attL*cgcK:4*ttgV:4*agaT:1*ccaS*gagQ*acaD*gctP:3*atgY-gttaag*gatG:1*tcaN*tccP*gtcE:1*gagG:1*actA:1*agaA*ccaL*gaaP*tctK:1*cagP*acaI*aatS*ttgE*tcaV*gaaA*ccaS*attL*tctA*gagT*cagG*ccaA:1*gtcT*tccA*cagP*ttcS*cagT*caaT*ccaD*attL*gctT*gcaP*caaE:1*actV-ggt:5*aatT+PQGQQQLQQQQL:2*tctQ*agtQ*tttY:1*gctE:2*attR:2*caaA*caaA*gagQ:1*gatE:1*caaE*gctL*ctcQ:3*cagM*tatL*gctH*gaaL:2*agaQ*attQ*ttgQ:2*cagE:2*gtcR*caaM:1*caaA:4*attL:3*caaA:3*gagD:3*tcaR:2*cagD:2*gtaA:6*ctgI:1*gctN:6*caaR:3*ctaM:1*cagE:3*cagR:3*gccG:2*actG:1*attL*acaQ*gctQ*accV*actI*caaA*tcaN*gctT*actN*cagL:1*attS*ggcQ:3*gagD*ttgM:1*caaR*aacS:1*catQ:2*ataV*actN*ctaS*tggL:1*aacS:2*gagD*agtV:13*aacD*ttgK:1*aatK*aagE*ttcL:1*aaaL:1*caaK*caaV:9*gacE:6*aagR:1*ctgI:1*tcgA:2*gtgI:3*ggtD:9*ttgY:2*gagD:1*gttS*aagR:1*ggtN*aaaQ*ggtK:1*gagV:1*gacE:3*ttgR:1*attL:1*gatL*ctcT*accE*ttgD:2*gatA:4*aacS*caaK*tcaG*tcgA*aacD:2*actL*attL*tttI*gctS*caaK:1*aagN*aaaR:1*atcL*gaaA*gctD:2*aacE*cagA:1*agaK*cagA*caaK*tctQ:1*gctV*ttcL+DNYANRSFD*aatD*ccaS*gaaD:1*gaaK*caaL*ttaK:1*aagA*gatE:2*gaaD:6*caaE:1*atgV:1*gatS+MEA:2*gtgK:1*ttaM*gccS*aaaL*gctN*cagH*aagN:1*aatA*gaaD*acaQ:1*attL:5*caaE:2*gttK*gatE:2*acgS:2*actN:1*ttgI*gttI:1*gctS*atcV:1*ctcE:1*ggcS*ctcA*aagE:1*ttaV:1*gacE*gccS:1*tatF:1*ttgF*gacE:10*tcgT:7*atcV:4*tccT:2*atgT:2*ggtA*gacT:3*gacN:9*cacY*gctS*gagG:2*cgtS*tctT*ctcA*aatT*gacI*ttcL*actS:1*tccV:1*gctP:3*ttaN:1*tctA:2*attL*gtgT:2*gttA*aaaR*tcaD:1*gcaT*gagV:2*caaN*ttcI:1*gctS*actY:2*gctL*actA:1*gagV*gaaA*tcgV:1*gaaD:2*aaaS*tctG*ttgV*cttQ:1*gatN*agtK:1+A*gagQ*ggcL*gaaS*gtgD:1*gagD:3*gtgK:1*atgI*gccN*ggaF:1*ttgI*gcaD:2*gagT*aaaH:2*gatS:1*accS:1*ttgV*actS:1*gacK:1*gccT:2*tctA*aatG*cttT*ttgH*aacL*agcN*aacG*gttS*gcgG*aacD:5*gaaD*actR:1*ttgM*cagA*aatR:2*gccE*tccA:1*tcaE*aagS:1*tcaN*tccA*cagK:1*actN*tcgE:3*aaaR*gatP*caaK:2*tctK*atcF:1*caaV*atcF:1*tttL*gagK:1*aacH*aaaE:2*gttL*tccA:6*aatT*tccA:1*gaaA*cttA:5*attS*gctD*tcaC:1*gagQ:4*aagQ*aacG:2*tcaA*attS:1*aagR:1*gaaA*tacF:19*cttA:2*aagN*attV:12*aaaH*tctT*catP:7*catN:3*gctS:2*gttA:10*acgS*tttY*ttgM:5*aatR:2*actG:3*aacA:16*actS*cagK*agaK*agcQ*aacQ-ggcggt*agtK*ggtE:10*aatP:1*gaaV*aacL:2*gtgT:13*gccS:1*atcN*gcaS:11*ggcS:1*agaV:1*gggD*gacE*agtD*agcE:1 +PPLX03000005.1:95524-162156 miniprot mRNA 24980 28114 2844 + . ID=BC000022;Rank=1;Identity=0.5523;Positive=0.7224;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +PPLX03000005.1:95524-162156 miniprot CDS 24980 28114 2844 + 0 Parent=BC000022;Rank=1;Identity=0.5523;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +##PAF 4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1053 0 1046 + PPLX03000005.1:95524-162156 66633 24982 28114 1623 3168 0 AS:i:2570 ms:i:2570 np:i:744 fs:i:0 st:i:0 da:i:0 do:i:6 cg:Z:99M4I201M1I7M1D7M4I265M1I39M5D134M1I39M2D33M1I153M2D57M cs:Z::2*aagS*acgN*atcR*tctA:1*tctV:2*cagN*actV*gccN*cttI*cgtK:3*tctN*gctT:2*actS:14*tggH:2*aagR:2*ctcK*tcgA:3*gctG*ttaI*aagR:1*ttgQ:1*attL:1*aatT:1*gaaD:1*cagM*ttaV:15*caaP:3*gcaR*gaaD*ggtA*tacQ*aggA:1*ttgV:1*tggF:1*gatQ*ggtS:1*gggA:1*tctG*ataN*caaN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aaaS:3*accR*tacF:1*atcM*agaQ:2*cagT:2*cggK*aatY:1*cgtP*ggtA:3*accM:4*gagD:4*gtgR:2*caaN*aacD:1*gatN:7*gatN:2*tccT:3*tcaG*cttV:2*ctgF:3*attV:1*gcaS*acaS*attL*tcaQ*catR*gatS*aagR*agaS*tctN:5*gcaS:3*cttM*gttI*actA:6*aagR:1*atcV*accI:5*attM*tacH*cgtV:3*tctN:1*gatE:3*ccaS:1*aagR:2*tacF*gagY:3*gctD:2*ttcR*gaaD:2*gctY:3*tccT*attL*cgcK:2*actR:1*ttgI*atcV:3*agaQ:1*ccaG*gagK*acaN*gctP:3*atgL*gttE*aagE*gatE*gaaD*tcaG*tccP*gtcG*gaaL*gagP*gagQ*actR:1*agaK*ccaS*gaaV*tctA*cgtE*cagT*acaR*aatS*ttgV:1*gaaP*ccaE*attP*tctA*gagP*cagL:1*actV*gtcA*tccT*cagP*ttcT*cagP*caaS+I:1*attA*gctE:3*actI-ggt*gtcE*gatN:3*aatQ*cagD+ALLA:1*tctQ*agtQ*tttY*gagD:3*attE:2*caaR:1*gagQ*cgtQ*gatA*gcaM*caaE*gctE*ctcE*caaR*caaI*caaR:1*tatM*gctQ*gaaM:2*agaQ*attQ*ttgQ:2*cagM:2*gtcR:2*caaM:1*cagA:1*cgtQ*attR:5*cttM*cgcA*gagD:1*tacM*cagA*tcaR*caaH:4*gtaM:6*ctgI:7*gagD:4*ctaM:1*cagE:3*cagG:6*actA:1*attL*acaN*gctQ*accL*actQ:1*tcaT:1*actH:1*caaS*attA*ggcQ*tccA:2*gagD:2*caaE*aacS:1*catQ*gagQ:3*ctaM:1*aagR*aacQ:3*agtT:3*ctcR:2*caaS*ttgM:1*cagE:1*cacY:1*aacA:2*aatK:1*ttcL:1*aaaA*ctcT:7*caaK:3*gacE:1*cgtA:4*aagR:1*ctgM*aaaR*tcgH:2*gtgI:3*ggtD:2*cgtK:6*ttgY:2*gagD*aaaR*gttA:3*aaaN*ggtK*gatE*gagD*attV*gacE*aagR:2*ttgR*caaE*attL:1*gatM*ctcA*accQ*ttgD:2*gatA:1*gccK*gagD:1*aacS*caaT*tcaG*tcgA*aacD:2*actL*attL*tttL*gctS*caaK:1*aagN*aaaR:1*atcL*gaaS*gctE:3*cagA:1*agaK*cagM*caaK*tctQ*tcgR:1*ttcL*aatD*ccaE*gaaR*ttgG*gaaD*caaD*ttaS+D:1*aagL*gatR*aagR*gatL:2*ttaK:2*ctcE*caaY*caaE*acaA*atgL*gacN*gatE:1*ctgF*gtgN*gaaS:2*aaaL*gctH:1*aagE*gagQ*aatQ*gaaG*acaN*gctN*attS*gaaN*gagG*cagG*atcS*gacT*caaP-gttttggttgatcac:1*acgA*aagA:1*actH:1*ttgI*gttI:2*atcL:1*ctcE:2*ctcS*aagQ:1*ttaV:4*tatF:2*gacE:10*tcgT:6*attV:5*tccE:2*atgS*gacA:1*ggtA*gacT:3*gacN:1*atcL*attA:1*gggE*ccaT*aatD:2*cacY:4*cgtK*tctT*ctcV:1*gacI*ttcY*actS*tctT*tccA*attV*gctE*gacN:2*ttaS:4*attL*gtgT:2*gttA:1*tcaD:2*gagS:2*caaA*ttcL*ataV*gctN*actF:2*gctE*actS:2*gaaA*tcgT:1*gaaR*ttcN:1*aaaI*tctG:3*gatE*agtI*ttaI:1*ggcD+F*gaaP*gtgL*gaaD*gagD:1*actM*gatE*gtgR:1*atgI*gccT*ggaL:1*ttgM*gcaN:2*gagT*aaaA:2*gatH:2*caaK:1*actA:1*gacR*ttaM:2*agaK*tctT:1*cttI*ttgN*aacM:1-aacgtt*gcgG*aacD:6*actR:1*ttgM*cagA*aatK:2*gccD*tccA:1*tcaA*aagA:2*tccA*cagK:1*actG*tcgD:2*tcgK*aaaF*gatN:1+S:2*tctL*atcK:1*caaT*atcT:1*tttL*gagQ*attL*aacH*aaaE:1*attA*gttI*tccQ:2*attQ:2*accI:1*tccA:1*gaaA*cttA:2*aaaR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aaaR:1*tcaT*attS:1*aagR*actA*gaaQ*tacF:3*cacN:1*cgtK:13*cttA:2*aagN:4*actK:3*gttT:1*tctR*gggR*aatT*aaaS*tctG*catL:1*cagE:5*catN*gagN:5*gttA:12*ttgM:5*aatK:2*actE*gcgC:2*aacV:6*agtN:2*aatK:3*gacE:2*actN*cagK*agaK*agcF-aacggc:1*agtE*ggtL*gagD*aatE*gatK:3*tcaA*aaaA:1*acaS*aatK:2*aacF:2*gtgT:13*gccD:1*atcQ*gcaG:6*gaaQ*attM:2*tatV*ggcA:1*agaL*gacH*gggQ:1*agtE*agcE:1 +PPLX03000005.1:95524-162156 miniprot mRNA 24983 28114 2570 + . ID=BC000023;Rank=1;Identity=0.5123;Positive=0.7045;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +PPLX03000005.1:95524-162156 miniprot CDS 24983 28114 2570 + 0 Parent=BC000023;Rank=1;Identity=0.5123;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +##PAF 4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1054 0 1047 + PPLX03000005.1:95524-162156 66633 24982 28114 1620 3171 0 AS:i:2556 ms:i:2556 np:i:744 fs:i:0 st:i:0 da:i:0 do:i:6 cg:Z:99M4I201M1I7M1D7M4I265M1I39M5D134M1I39M2D18M1I15M1I153M2D57M cs:Z::2*aagS*acgN*atcR*tctA:1*tctV:2*cagN*actV*gccN*cttI*cgtK:3*tctN*gctT:2*actS:14*tggH:2*aagR:2*ctcK*tcgA:3*gctG*ttaI*aagR:1*ttgQ:1*attL:1*aatT:1*gaaD:1*cagM*ttaV:15*caaP:3*gcaR*gaaD*ggtA*tacQ*aggA:1*ttgV:1*tggF:1*gatQ*ggtS:1*gggA:1*tctG*ataN*caaN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aaaS:3*accR*tacF:1*atcM*agaQ:2*cagT:2*cggK*aatY:1*cgtP*ggtA:3*accM:4*gagD:4*gtgR:2*caaN*aacD:1*gatN:7*gatN:2*tccT:3*tcaG*cttV:2*ctgF:3*attV:1*gcaS*acaS*attL*tcaQ*catR*gatS*aagR*agaS*tctN:5*gcaS:3*cttM*gttI*actA:6*aagR:1*atcV*accI:5*attM*tacH*cgtV:3*tctN:1*gatE:3*ccaS:1*aagR:2*tacF*gagY:3*gctD:2*ttcR*gaaD:2*gctY:3*tccT*attL*cgcK:2*actR:1*ttgI*atcV:3*agaQ:1*ccaG*gagK*acaN*gctP:3*atgL*gttE*aagE*gatE*gaaD*tcaG*tccP*gtcG*gaaL*gagP*gagQ*actR:1*agaK*ccaS*gaaV*tctA*cgtE*cagT*acaR*aatS*ttgV:1*gaaP*ccaE*attP*tctA*gagP*cagL:1*actV*gtcA*tccT*cagP*ttcT*cagP*caaS+I:1*attA*gctE:3*actI-ggt*gtcE*gatN:3*aatQ*cagD+ALLA:1*tctQ*agtQ*tttY*gagD:3*attE:2*caaR:1*gagQ*cgtQ*gatA*gcaM*caaE*gctE*ctcE*caaR*caaI*caaR:1*tatM*gctQ*gaaM:2*agaQ*attQ*ttgQ:2*cagM:2*gtcR:2*caaM:1*cagA:1*cgtQ*attR:5*cttM*cgcA*gagD:1*tacM*cagA*tcaR*caaH:4*gtaM:6*ctgI:7*gagD:4*ctaM:1*cagE:3*cagG:6*actA:1*attL*acaN*gctQ*accL*actQ:1*tcaT:1*actH:1*caaS*attA*ggcQ*tccA:2*gagD:2*caaE*aacS:1*catQ*gagQ:3*ctaM:1*aagR*aacQ:3*agtT:3*ctcR:2*caaS*ttgM:1*cagE:1*cacY:1*aacA:2*aatK:1*ttcL:1*aaaA*ctcT:7*caaK:3*gacE:1*cgtA:2*ttgM:1*aagR:1*ctgM*aaaR*tcgH:2*gtgI:3*ggtD:2*cgtK:6*ttgY:2*gagD*aaaR*gttA:3*aaaN*ggtK*gatE*gagD*attV*gacE*aagR:2*ttgR*caaE*attL:1*gatM*ctcA*accQ*ttgD:2*gatA:1*gccK*gagD:1*aacS*caaT*tcaG*tcgA*aacD:2*actL*attL*tttL*gctS*caaK:1*aagN*aaaR:1*atcL*gaaS*gctE:3*cagA:1*agaK*cagM*caaK*tctQ*tcgR:1*ttcL*aatD*ccaE*gaaR*ttgG*gaaD*caaD*ttaS+D:1*aagL*gatR*aagR*gatL:2*ttaK:2*ctcE*caaY*caaE*acaA*atgL*gacN*gatE:1*ctgF*gtgN*gaaS:2*aaaL*gctH:1*aagE*gagQ*aatQ*gaaG*acaN*gctN*attS*gaaN*gagG*cagG*atcS*gacT*caaP-gttttggttgatcac:1*acgA*aagA:1*actH:1*ttgI*gttI:2*atcL:1*ctcE:2*ctcS*aagQ:1*ttaV:4*tatF:2*gacE:10*tcgT:6*attV:5*tccE:2*atgS*gacA:1*ggtA*gacT:3*gacN:1*atcL*attA:1*gggE*ccaT*aatD:2*cacY:4*cgtK*tctT*ctcV:1*gacI*ttcY*actS*tctT*tccA*attV*gctE*gacN:2*ttaS:4*attL*gtgT:2*gttA:1*tcaD:2*gagS:2*caaA*ttcL*ataV*gctN*actF:2*gctE*actS:2*gaaA*tcgT:1*gaaR*ttcN:1*aaaI*tctG:3*gatE*agtI*ttaI:1*ggcD+F*gaaP*gtgL*gaaD*gagD:1*actM*gatE*gtgR:1*atgI*gccT*ggaL:1*ttgM*gcaN:2*gagT*aaaA:2*gatH:2*caaK:1*actA:1*gacR*ttaM:2*agaK*tctT:1*cttI*ttgN*aacM:1-aacgtt*gcgG*aacD:6*actR:1*ttgM*cagA*aatK:2*gccD*tccA:1+A*tcaA*aagA:2*tccA*cagK:1*actG*tcgD:2*tcgK*aaaF*gatN:1+S:2*tctL*atcK:1*caaT*atcT:1*tttL*gagQ*attL*aacH*aaaE:1*attA*gttI*tccQ:2*attQ:2*accI:1*tccA:1*gaaA*cttA:2*aaaR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aaaR:1*tcaT*attS:1*aagR*actA*gaaQ*tacF:3*cacN:1*cgtK:13*cttA:2*aagN:4*actK:3*gttT:1*tctR*gggR*aatT*aaaS*tctG*catL:1*cagE:5*catN*gagN:5*gttA:12*ttgM:5*aatK:2*actE*gcgC:2*aacV:6*agtN:2*aatK:3*gacE:2*actN*cagK*agaK*agcF-aacggc:1*agtE*ggtL*gagD*aatE*gatK:3*tcaA*aaaA:1*acaS*aatK:2*aacF:2*gtgT:13*gccD:1*atcQ*gcaG:6*gaaQ*attM:2*tatV*ggcA:1*agaL*gacH*gggQ:1*agtE*agcE:1 +PPLX03000005.1:95524-162156 miniprot mRNA 24983 28114 2556 + . ID=BC000024;Rank=1;Identity=0.5109;Positive=0.7039;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +PPLX03000005.1:95524-162156 miniprot CDS 24983 28114 2556 + 0 Parent=BC000024;Rank=1;Identity=0.5109;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 diff --git a/results/2026-10-30_barnettozyma/data/GCA_003706655.3_orf_scan.tsv b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_orf_scan.tsv new file mode 100644 index 00000000..e7220e51 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_003706655.3_orf_scan.tsv @@ -0,0 +1,9 @@ +seg strand wstart wend aa pfam best_ref +s229 + 24968 28120 1051 ANTH:260:14-275,I_LWEQ:4:848-872,I_LWEQ:195:891-1043 sla2:1217 +s266 + 41747 42265 173 MATA:33 +s422 + 41346 41699 118 460519_cbs-7435_MATkp_MATa_MATa2:16 +s449 + 49620 50618 333 dic1:362 +s551 - 35440 35778 113 MATALPHA2:18 +s689 - 40293 40802 170 Homeobox_KN:27:136-165,Homeodomain:53:112-167 460523_cbs-4732_MATog_MATa-MATalpha_MATa1:58 +s705 - 35334 35441 36 Homeobox_KN:19:13-35,Homeodomain:17:8-35 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha2:18 +s849 - 36758 37462 235 MATalpha_HMGbox:33:93-174 MATalpha1:62 diff --git a/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.a2.gff b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.a2.gff new file mode 100644 index 00000000..879555f8 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.a2.gff @@ -0,0 +1 @@ +##gff-version 3 diff --git a/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.fa b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.fa new file mode 100644 index 00000000..03b7dd34 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.fa @@ -0,0 +1,954 @@ +>JAMAIE010000005.1:589225-655853 Barnettozyma californica strain PB4207 ctg_5, whole genome shotgun sequence +TTTTTTAAGGCCTTGAAGATTTCTGACTTATTAGTTAACAAATCATTTAAGAATCTGTACGTGAACATAT +CATTGATATTGATATTGTTCGAACTTTTTGGTCTTAAAATGCAATTAGAAAACTCTTGACTGATCCCATT +TCTGAATGTCTTCCATATCGCGTCATTGTCCTTGTTTCCCTCCTTCAAAATAGAAATTAGTCTCTGGAAA +TGTGTGGCCAGTGCCTGCAAAGTTGCAATATTCGGTCTACTCAGATCCTTTAGGACATTGATAATACCAT +TGATGCGACTTTTCTCGTCGCTATCAGAACCAAACTGATTATAGATAGACTTGATCAAATCGTAGACTTC +GCTAGGCACAAGGGAGTCCGGTAACTCAAGGAGGTACAGCTTCAATACACTCGCAACAACTTCAGGAGGA +TATTTCGACAATAGTTCATCACTAAATGTCTTGGTACTTTCAATCTCCCTTCTCAGTTGATGTGTACTTT +GAAGTTTAACTGGGATAAGCCAGATCTTGGTTCTCACCTCGTCATTAGGCAACTCCGGATAGATGTTGTC +CATATATGAGAGAATCGATGATACAATCAATGGCACAGTCTTTTGGTCGTTCTTGCAATGGAATTCCAAA +TCCACACCAAAGATCTGATCTTTGAAACTATCATAGTAGTTATCGTAAAGAACGACTTTTGGTGCGAATG +ATCCCGTGCGGTAGCTCTGGAGGAAAAATGAGAGATCCTTATCTGGGTGGATGGATTCCTCATACACTAA +AATCTTATCAATGGTGGATTTGATTGCAGTGATGTTGTTGGAAATCGAAGCACTGAAATCCAAAATGACC +TTTTTCAGAGCTTTAAGACGATCAAGTTCACACTTTTCCATGAACGACAAATGATCAAGAATCAGCTCTT +CAAGGTCACATTTTATCTTGTCCAGTTTCTTTACTTCTAATTGGTAGGCCTCATCTAGTTCTTCAATTTC +TTTATGTGCTTTTCTCAAGGTAGGTTCTTCGAATTGGGACTTTAGATCATCAAGATATTTGGTCATTGAT +GCTGTGGTGCTACTTAATGACTCTGAAGGGTTCCCATTAGTACTGGCAATAGAGCTAGCCCTCTCTCTGT +TGTATCCTGATATTTCAAAAGCGGCATCACGCCATTGATAAGCAAAAGAACCACTATTAACAAACATCGT +TGAATTAGAACCAAATCCCATAGTGGTATTGACCTTCTTCAGGTATCCGTTGGCAATCAGATCCTTACCA +AAAGTCTCACATTTGTCGATGCTAGAAATTCCAAGATTCTCTTGTATCCATGAGACAATTGCCGATCCAG +TGGAACAGTTGTTGTAAACACCAATGATTGGCACCTTGATTGATGTTTGAGGTATCTCAGATAGCATCTT +GTGCAACAGATCCTTGAGACCAATCTTTCCATACTCAATACCACATAATACTACCAACGTTTCGTACTCC +TGCTCTACTTGTGGCTGCTCCTGATTTGACACTGATTTCTCCTCTCCATCACTCTCATCTTTGATGCTCA +ATTCTTTTAGTACTTCCTCTTCAGACAGTCCATGCTTCAGAGTCTCATATACTCGACATTTGCTAAAGTA +TTTCTTCTGCACCTTTTCCACAACTTTGTATTTGTTCTTGTAAACTTTAAGACTATCCTTTAGAATCCTC +TCAGAATACTGCACCCTTTGCTTGTGCTCTTGCGACCACTTCGAAAAAGGATCAACAACGAGCTCTTTGA +TATTCAGTGCGATTTGCAGATGAACGCGACCCTCGTTACTCATCTCTTCTGTCACACCTTTGAATGCGTT +CTTCAAGCTTGCCCCATCGTCCTTGTCAAAACCGTGTTTGCTTGGCAAGTGGAGGTCAGGAAGAATCAGC +AATTTGCGGCCATAGTCTTCCTCATGTTGCATACGGGCAGAGAAAAGATCGATGAACTCGTCATTCTCAA +CACAACCTTGATTCAACTTGTTAAAAAGCACTTCAATACCTGAACACGATGTTAGTATTTAAAGATTGAG +GAAGGTTAAAGGGTAGAAAATAACTCACCTGTTGAGAAATCAGGTGTCCAAAAACTATCTGCAAAGGATG +TCATGTCTCTCGCTGCAATCAACAGCTTTGGATCTAAACAACTCACTTCAACAATAATGTTTTTATAACT +TTCTTATTGCCAAATCATACAGGAGTGTCAGAATTTGGAATTACGGTAGGCGCAGCATAGTCACGTGATT +GGAAGGGGATGTTTATTTAAAGATTTCACCTACTAAGGTTGAGAATAGCAAAGGTCCTTATCATTATTAC +AGATTATATACTGAGTAAAAAACAAATAACTCTTCTGCATTCATCACTTTTGCTGCTGGTTCAAGAACGA +CAGCAACTGGCTCAATTGCTCGTTTGTTTGAGCCGCTGGTGCTGGTGGTGCCTGCACAGGTGCCGGAGCA +GGAGGCCCCTGTTGTGGTGGAGCACCGTTTCCACCGAACAAGTTAGACAGCACATCACTCTTACCACCCT +GCATGAAGCTTGGCAGCTGGTTACCCCCAATAGGATCAGTGTACATATCGTCTGGCTCGCCAGGTTTAGT +AGATCTTGGACCATTACGGGACCCATCTGTAGGCATCTTTTGAGAAATAGCCTTTGAGGAGACACCTTCA +CCGATTTCGATATCTGGCTCTTCCATAATTAAGCCAGGTACCAGTTGTTGCTCTGTCCCAATACCACCCC +ATTGAGCCTCGTTGATCCAACGTCTGTCAGCATCCGTCAAACGTGCAATAGGAATAATGGACATACCATG +TTGGTAATCACAACAGTCACGAGGGCCGAAACCAACACCCCAACGGGTACGCAGACTGTATTGCCCATTC +TTGTTGAAAGACGAGACAACCATCTCCGCCTCTTTTCTGGAGTACACCTTCACAAAGGCGTGTTTACGCT +CAGCGTTGAGAATAACGGACTGCACCTCCGCATATGGTCTCAAGATCGCAGCCAGTTCTTCCTGGTTCAT +GTAGGCAGGAACACCACCAATAAACAATGTACGACTCAGAACTTTAATAGAACCAGCAGGAAGAGATTGG +TCAAATGACACATTGCGCTCACGAAAGTGTGGTGTGCCTGGCAAGTTGACCTCACCAATAACATGGCCAG +GGGGAGCGCGATCTTTCACTGGACGACGCGGTGATCTGTCACGATCACGACGTGAGAATCCTGACTGTCT +TTCGCGGAAGCTTTCAGCTGCATGACTACCACCATCCTGTTGTCTAAATCTGTTGAATGCTTCCTGTTGC +GAAGGAGGTTGTGGCCCGCGGGCCCAGCGCGATTGTGTTGAAGGATCCTGTTGTGATAACTCTTGCGGCG +GCTGTTGGGACGAAGGCTGCAATGGGGGCTGTGGCATTGGAGGGATCTGTCCAGTCGACTGCATGGCTTG +TAACATTTGGAAAAGAGCAGCTTGTTGCTGAGCTGGGTCAGCTGTCGCTAAGTTACTAAGATTTGGTAAT +GCTGGTGCTTGTGCTAACGATGGCACACTACTGCCAGAAGAACCTGGAGCGCTGGCTCCTGGAGGTGGTG +GTGGCACGGCACCACCCATAGAAGCCAACTGTTTCAACAAATCGTTAGCTTGAGAAGCTGTAGGGACTGC +TGGGGTAGCAAGAGAAGGTGGTGGGACAGTTGGTGTAGTAGAGCCTAGTCTAACACTAGAAATCAAAGAA +TTATCAAACGTACCAGTCTTTCTCCAGATCTCGAGCAACTTAACAATCTTCTCCTTATGTTCCTCACTCG +CATTTTCATGAGCAATCTCAATGACCTCCTTCAAACTAGCAGCAAATAGAGAAATCGCCTTGTTCAAGTC +CCCAGTAGCGTCCTTGCCGGCTTTAATGTCCTCGTGAAAACCTCTAGCAATCGAGTCCAGCACATACAGG +GCACCCAGTTTATGACTAGCGGATGCACTTCTGATGTAGTTTAATACGGCAGGAACGAACTCCGTATAGA +GAGCAGGGAATTGTCTGGCAATGTCAGTTAGATGCTTGATCTTCTTACCAGATATACCGTTCTTGGAGAC +ACGAATCTCCTCAATGGCCGCATTAAAAGCATCCATTTTATCACTATATCGACTTTGTTTTTTTTTGTTT +CGAATTTGGAGAAAAATAGCTCTCGAGGGGATGTACTGAGCTGCTATGTGGCCTTTGATTGAAGCAATGA +GTGTACGGTCCTACTCTTACTTTACAATGCGTGTGCTACTTTAACGCTGATGCTGTTGATGTTTGTGATT +GGAGAGAGTTAGCGGCAAATTTTCGTTGAAGTCGCGAGAAGTCACCACCGGGAATTAATAAAAAATAATC +CGAGGGTACTATTTTTACAAAAACGGAAGAAGTCCCAAACCGGACATTTTCTCTAACATTTATTTTCTTT +TCACGCAACAGGCTCATGACAGCATCGAACGTTATAATGGCTAGTATCGCACGGAAATCGCACTATGAAA +TACTCTCTGTTGAGAGGTGTGCTTCATTTGCAGAGATCAAGTCAGCATACCGCACGAAACTTCTAAATAC +TCACCCAGACAAGACAAACCAAAGCATACAAGGCGACCTGATCGCCCAGATCAAAGAGGCTTACAAGGTA +CTATCAAACGAAAAGCTACGAAATGAATACGATGCAGAACTGACAGAGATATTCAAGAAGAACGGGACAC +TCAGTACTGGAGAAGGTCTCGATGTGTTCACATTAGATGACTTCAAATGTGAAGAAGATGGTGATATTTG +TATATTCAAGAAAGATTGCCCTCGGTGTACATCCGAGGAGGGATTTGTGTTGAATGAGGTTGATTTAGAA +GAGAATGGTACCGAGGATGGAATGGGTGGTTATGAGATTATTCTTCAGTGCTCTTTCTGCAGCTTGTGGC +TAAAGGTGAAATACTACGATGCTGAGGAGTAGTCATGACAAGTGATTCATCATTATAATGCTAGAAGTTT +TCAAGAAATGATTGCCTCAAGTGCTTGTTCCATAGCCTCCAAGTCGCACATTAGCGAGATATGGCCCTTG +TCACTAGCAATTTTGGCATTAACAGGGAAGCCTCTGCGCTCAGGCATCAACCACTTGTGGTTTACAACAC +CATCACCAGGGCCATAGAAGAAGTCATCATACTCCCCGCGTTTGATACCTTCTGGTGACTCAACCCGCAC +ACCACGAACTGTAGGGACCTCATTGCCATAAACGATTGCCAGTGGTGGATATTCCTTCGTTGGGTCGTAT +TCTAGCTCTAATAAGAATTTTTTGGTCCTCTTTAGTGTGTGCTCAAGATATGTTTTGGCTTCCTCAAATG +ATGTAGAAAACTGATATTCGGTAATTGGGCTCGCGGGGTTTGCCTCACCTTCCAGTCTGTCTTTGGAAAC +AGGAGGTGATAGGTTGTATTCGATCCATGTGTTCACATCAAAGTAGTCCAAGGTGTATTGCTCATGTGTC +CTTCTGTTCATAAAACATCTTCCCTCAAGTGGCAAGAAAACAAAACTAGATCTCATAAAGAAGTTCACCT +CTGCGGTTAGGATCTTGGAATTCACAAGGACTCTGTCCCCAACTCTGATTGGACCCAGAATATTGGGACA +TTCTGCAGGAACTCCTGCATACACCAATCCTCTAAATAAACTTGGATCCTTTTGCATAGCATGATGAGCC +ACCAAACCACCCATTGAGTGAGCAATCACAATTGTTCCCTTTCCCACAATTCCCTTTTCCGCTCGCTTTC +TTTCCAACTCTTGAAGAAAGTCGAACAGCTTTTGCGAAGAGATATCGGAAGAAAGTCTCCAGTCGTACCC +AAACGCATGGACATTGGTTTTCCCATTATTCAATTTCGACATCAGCTTCCTGCAAATGTCAATGGGCCCA +ACATGGGACATGATATCATCTGAATAGATCTCCTTCTGTGCTTTTTCCTCATCTTCATCTGTAGGTCCAA +TCAAAAGATCAATCTTTCTGATGTTAAATCCAGCCCTTATAGGAATCCACACACGTTTATGAGACTTGGT +CTCCCTCAAGATGGAGCCTCTGTACCCACCTAGGATCACAACATCACCGTCAATAGTCTCCCATATGGAC +TCATGCTTCTTCTTATCATCAAATGACGGCAGTGAGATGTTTGGGGTAAGCGCATTCGTCACTGCTTTGA +GTCTAGATGAGCCCTGCCCTTTAAGACCATGGAATAAATAAGCCTCCTCCAAAGTGTTGATGGTTTCTTG +TCTCTTCAACTTGTTGCGGATCTCCTCCTTCGATAAACTCACGTTAAATTCATCATCTATGACATCTGTG +CTACTGTTACCGAAAATTGGGATGCCCTGCGTAAACGTTCTCAGTGACGGCAAGTGTGACAAAGGGTTGC +CATTGCCTCCAAAAGGTAGACTAAAAGTGAAAATCTTGTGCTGATTCAGAAAATGGCCCCTCTTGAGATC +ATCTGTTGTCACTTCAGGGTCAGGTGACTCTCTATTACTTCCACCAATCACACTTGAGCCGCCAATTATG +GAGTGTGCTGCTTCTTCCCCCAGATTCTCACCATCTTGGGAATCTACATCATCTGAATCCTTCAGGTCAA +GACCACGGAAGTCCTGGATCGTGCTATCAGTAGGGGACGTCTCTGCTGATGAGAGTAGATTTCTAATATC +CTTGCGAAGTTCAAGCAGCATATTATTTGATAATTGCATTGGATCAGACTTCCGCGGTGAAGAATTTCAG +TTCCTTTTTATCCCATTAAACCCTTCGGTCTTCGGCCTCGGCCTCGGTTCTATGCAGAAAACTTCACCGA +TCTCCAAAGACTCCCGTTGTCGTAGCAACAAACAGGAAGTTGGATAAAGCATCGCTAGTCTACAACATGG +ATTCGGACGACAAGATACTTAGACAAGGTTTATCTTCTACCATCCCTTCTAAACCTCCTTCCACCATCGC +GTTTTCCATCCCTTCTTTCGCCATCCCTTCTTTCGCCGTCCCTTCTCTCTCCATCTCTCTCCCTTCTGTC +ATCCCTCCTATCGTCTTTTCTCCCGTCTCTTCTTTCATCCTTTCTATCGTCTCTTCTGCCATCCTTTCTA +TCATCTCTTCTCGTAAGAGGGTCACGTCTTACTTCATCTCTTCTTTCCTCTTTTCTTCTCTCCTCCTTTC +TAACATCTCTTCTTATTCCTTGATCATTCTTATCCATCTCATAAAGAATAACTCTACCATCCTCCACACA +AGCAAAACTTGATGTTTTATTCGAAACAAACTCCAACGCTTTGGCCTTGATCTCATGAAGCACCTCTGAG +CTTTCCACGCTGTAAAGCTTACAGATTCCATCATCACTCTTTCCCAAAGAATAGGAGACTGCAATTGTCG +TGGCGTCCTTATTGACACAGACATTCACCACTTGTTCATCAACATCACCAATGGTTTGTGTCACCGTTAA +AAAATTCTTTTCATCCAATGACCAAAATGAGATGATTCCCTGGCTAGATCCAGAAACAAGATCACGACGC +GGGCAGAATTGTAGGGTTGTAATTGGAGAATTATGACCAGTCAAGGTTTGCAATAACACCCAATCGGAGG +GAGATGAGCGATGGTATACTCTAATATCACCATTGGTTAATCCCACAAAAAAGAACGCTGCGGTATTCGC +CCAACAAATACAAGATATAGTTGCTGATGGTCCCATATCAATGCTTTGCACCAAATGATGGGACTTATCA +ATGATACAAACGACACGTTTTGACGATGAGGCAATAAACAGGCCATCTTTGGAGCAGGCCACATGGCTGT +TGCTCACTTTGGTATCTATTGTAGCAGTTCGTGAGCCACGATTAGCACTCCAAACAGATACTAAGTGCGA +ACCATCAACAGTGTATAGGACAGCCTCAGAATCAGGCTTCCATGTGAAGTCGTGAATACGACTAGCAGAC +GTGCGCAATGCTCTATTAGAAGCTGGATCTATCGTAACTGGGGGTTGTGAACCAACAAGGGACCAGATCC +GGAGGGTACCGTCATCTTTTGATGCTGCAATCCTGCTCGATATTGGATTGAAGTGGATCAAGAGAGCATG +CGAGCGAGTTGGATCAACGAGAGGTAGTGGTTTCATTGATTTGAAGTACTCTCCATTGTTTACCTGTGTT +TGGCCCCCGGTAGACATTATCGAATTGGTGTTGAACTCTTTGGACCTGGCTCAAATGACGGTTTCTAAAT +GAAAAAAAAAAAAAAATAAAAACAAAGGAAGATCAGCAAGAATTTTCAACAAGAGTTACAATGAGTGAAC +TGTGCGTCAATAAACGCTCCTCAACTAGGCTTCTGTTACTAACAGCGATACAGACAGTCTCTAGAAAAGG +CTTTGATAGGTCTTGCTTCCGTTGAAGAAGAGCTGGAACAAGCCGAGCGCGCTGCTGGTATGTGGTGTCA +CCAAACCTTCAACTGATTGTCATTACTAACATGTCCAAGAAATAGCACGCCTCCGTGCGGTTCAGAAGAT +CTACAAAAAGCGGAGTACATTCACTTCAGCGCTAGATAAGTTCTGGCACATTGTCCTGTCACAACACCCT +GACTTCTCTGAGTACATCCGGGCACAAGATTTCAAATACGTTGAACTGATCAACGACATCAGCGTCCAAT +GGGATATCAAGGAGACTGAGAAAGATTCAAAACCAGAGAATTTCAGTATAATTTTCAAGTTTGATGCGCT +TGATGATGACTTCCCTGCTCAAACGATTGTGAAGAAGTTTGAACTAGAAAAACTTGACCCTTCTGATGCT +GTTCAAGGTTATAGAAGTACTGCTGGTGGTGATGATGATGATGATGATGAAGAGGAGGAGCCTATTGCAG +AGCGTCTCACTAGTAAGGCTGTCGACATCGTGTGGCCAAAGTCATACGACTCAATCAATCCAAAGAAGAT +CACTGATAAGAAAACTCCAGAGGGAAAAAAAAACTACAGAACAGGGATGAAGTCATTCTTCGGATGGTTT +AAATGGACAGGGAAAAAACCTGGTAAAGAATTTCCCTCTGGCGATGACTTGGCAAGATTGTTTGCAGATG +ATATTTTCCCTAATGCAGTGAGGTATTACACAGAGGCGCAGAGGGATGGCCTAGACGAGGAGGTTGATTC +AGATGCCAGTGAACCTCTAGACTTGAGTGATGGTGATAATGATGAAGTACCTGCGGAGAAGAAGAGACGC +ACTGAATAGACTTAATTTGTTATTCGTTTTTTGAATCATGAGGGTCAAGAAGAATGTTAAAAGCGAAATG +TAAACAAACAAATATAGATTAGTTTACGTCGAAATTGGATACAGGAAGCGGCTAGCGCTAGTTATTAGAT +CCAAAGAATGTTAAACATGGCATTTAATGCAATACACAGCTTCGATATGCGTCCTTTGTTAAAACATTGA +TCTGTCAAACTGTTTTACATTCCCATTAGATCTACCCTTGTTGTCATGGTTAAAAACAAAAATTTTCAGC +TAAATTGAAAAAAACGGCGACGCGAGAAAAAAAAGAGGCCAGAGTTGGTAAACGGATCGAACACTGATCA +CCTAAATCTATAAAAAACGCATTGGCCAGTCATTGTGATCGGCTTTCATTATACTGAGATTATAGACACA +AGTACGAGATCCAATTCCTCCACATCGTGTCTCTGAAAGTGACGAATTCATAGAACACACAATTACTGGA +GTTAACCACAAATGACTTCGCCGTCATCTCCCGTGAAGAACAGTATCTTCATCCAACAGCACACATCAAT +GCCGACTACACAGACGCCCACACCTGTTGGACCATTAAGTCCCAACAAGACTTCCACATCTTCCCGATTC +ATCAACCAACTGCATGCTGAGGCGGACGAGCTAAAATCTGAAAACCTTAGACTCAAGAACAACAATGAGG +AGCTCAAGAAGAGCAATGAGCTGCTGAAGAAACGGAGAGATCAACTTATGGAACAGCTTTCCAATGCTAA +ACATGAGAATGATACTGTTAATTCACTCTTGAAAAGAAAAGAAAGACGTATCAATGACTTAGAGGAGCAA +CTAGGCAATTTGGAGTCCATATCTAACGATCAGGCTTTCAAGCTCAAAAACTTTGAGAATGTGAAGAATG +AGTTGGATAGCAATGTGGCCGAAGTATCAAGGCTTCAGATATCATATGATACAGTTGTGAGCAGTCAGAA +AGAATATAGAGATTTCTACAACAAAGAGATTCAGGAACTGAAGGAGAGATTGGGTGCATTTATGGAGGAC +AAAACAAACCTCATAAACAAGAACATCACTTTGGTGAACAAGAGTGATACTACTATTTTCAGATCGTTGA +AGTCCATCACACTGAGATCGAAAGAGCTGGAGGAAAAGTATACCAAGAAGGATATGCTATATTCCAAATC +AATAGACAAATTGACTAAGAGCTCAAATAAACATGTCGCTGAACTTAAGGCTTTAATCAATTCATCAAAG +GAATTGTTTGAAGAAGTGGGCAAAGTAGCTCAGATTGATACCAAGGAATTATTGGAAAAGTACATGTCCG +AGAATCCATTCAGCAGGGAGCCGTTCACTCAATCAACAGAGCCATCGCGTTCATCAACACCAGATATCGA +GTCTGGCACTATCAAGGTGACCAAGAGAGATGAGCGCAAGATTTCAAGATTGAATAGATCAAATGAACCA +GTGACTATCGAACAGAGAATTCGCTCACTTGGAAAGGAACTAAATGGATCCATCCCTCTCAAGGACAGAA +TCACACCTTTAGATAGTGTTAACAAGAGATCTCATTCTGGCACCAACCATCATCACAACTCGAAAGTGTC +GAATTCCAGGAATTCCTCTGTCAATCGTGATGAACAAAAACCAACAACTCCTACAGCGCCAACATCAGCA +ACATCGACAACATCGACAACATCAACAACATCAACAACAACTACAAAACCACAGGCAACAGATAATTCGA +CCTCCTCATCACGTCTAAGTTCGAATTCAGACCCAAAGAAGATCTTCGATGAGCCTGCAAAGGCATTGAG +AGATGTTTCTTATTCTGAACCAAGCACAACCAGAGGTGAACTGACTTCTACTCCTGCGGAATCCAGTGAC +ACGACCACTGATATCGGCGGCAATGGTGTTGACACTGCTGTTCGTGGTCAGCCATTGGTGTCAAAGTACA +TGGACACCAGTGCACTCCCAGTGGAGAACACAGAACCTGTTGAGACAGGGAAAAAGAAGAGAAAGAGAAA +GAGATCAAGAAAGAAGAAGAAGAGTGCTGGTGAGGATAATGACGGAGATGAAACGAAGGACGAATCAAAT +GAGGATGCTTTGAATGATAGTATGAAAGTCGACACTGTCCTTGAGGAACGTTGAAAACATAAAGCAGGAG +ACTTGAGATGAACGGACCACTACTAATCTCAGCTACGATGAGAAGGTCAATTAAGAGTAACTCCACCATC +AGCTCTGTATAGATGATCTACGCCGTTGTAGAAAGTAATGACTGTAGTAGTTGCACTGATCCGGTGTTTG +TTGAGTGACAGATCGACAACGCACGATGTACAGGGATCCGGGTGCAGCGAAGCCCTAACTACCAGGGTCC +ATAGTCCAATTGCGTCTATGGCTCAGGTTAGACTGACGAACTCAAGAAGAGTCTTAGGTATTCATGAAAA +TGAATATACGCTCCACATAGCATTACTCTATTGACAGAGAAGGTAAGACCAGATATTAGGAGTCCTACTG +TTCCAAATACGAACATGCACGGTTAACGGGGCATCTCAAGTTTCAAAAATTAGCGGACGCCGTTTAGCTT +GGTTAATCCGATGTTCGGTTTTGCAGTGAAATTGATGGAATCCGAACTTCGGAAAGAGTGGTCCGCTATA +CCAAGCAGATGCACACCTTTCTTGGGTTAAGTCAAGCAAAAGGAGGGAAGGAAGAAAAAAGCAGTCGGTA +ATATAAGATTATTCATGGAGCGTTCTGGTATGGTGGGGTCTTGAGAGGGGTTGTAGTTGATGGACTTCAA +CTCTCTGATTGGCCCAAAGTTCCACTCTGCGTTTCATGGACCGATTCCGGCAGTTTTTGGCGCAAACCCG +TCAAAAATTTTCTCGGACTTCTTTTGTCTCGGGCTGACGTAATCACAGCTTGCATCAATTCCTGGAGAAG +GGCTGCAAATTTTAGCCAATGGAATAGCCGCAAAATATAATCCTCACCTCTTGCACTTGGATTATTCGCT +TTTTAATGACTACAGGACACTGTCACATCGAGCAGTTCTCTGTTATAAAATAGGCACACAGGTTTAAAGA +TGCATTTCTCTAAGGCGGCAAAGTGTCCCTTGAAACACACTGATATTGACAACCTCGTGCTCCCCAAGCC +TAAGCTACTAAAGTCCTGTATTCGGTGTCGTAAACACAAGATCAAGTGCGACGCCTTTACCAAGTCACCT +AACCCATGCTCCAGTTGCGAAAAGAAGAAGATATCATGCAAAATCGACTATGTTTTACCACCTCAAAGAT +CAGACAATTTGAAAGACTTGATCTATCAAGTTGACTTTATTAGAAACGAAGTTGATCAACTTAATACCCA +CTATACAAACCTTTGTACTGATTTGAAACTTCAGCTACCCTCTTTCGAAGAGTGGAGGTTACAGCAGAAT +CTTGTTCCTGTCCAGGAGAGCACAAATAATTCAGGAGACGATGATAGTGAGGAGACACACAGTTACACAT +TAGAGGATAATCTTAATCTCATCAAGCTTTCGGATGGACGCTTTGTTTCTATGAATTTGCAGTTGAATAA +TGACCTGCTTATCATAGAGCAGGAAGAGGTTTCAATCTCTCAGTTCCAACATCGTTTGACGCTATGCTTG +AAAAACCTTAACTTATTTACAGGATTGTTTGAATGTGGAGATGAATCATCCTACGGTGCAGGTCGTTGGT +TTGATTTGGGGTTTAGCGTTCAAGAGCTTTTCAAGAAGAACAAATTCCAGCTGCTGATTCTTGTGAAGTT +CTTTTATCCTCATATCAGAATCAATTTTGAGAGATTCATGACTGATTACCTGTCTTCCTACTACAACATT +ATCAATCAGCTTAACCAGTCACTCCATACCTCTACGTATTCTTCTAATTTGAACATCATGATCATGAACA +AGTCATCGAATATTCTCAAGAGCAATGTTGCCCATGATTTAAAACAAACTATTGTCTTCAACAAGGAACA +ACTTTGCTCCTTGCTAAACGTGGAGATTTTCAACTCTGAAATTTTTTTGAAGAAACTTTTGCAACTCTTG +TTCCTTAACACGTGCCTTAACGGTGTCCAGCAACTCAGCTATGTCAATGAGTTGATCACCTTTATCAAAT +TGAACATTAATAATAACAGAAAAAGACTCAATTTTGAGAAAAATGTTGATCTCAAGTTCATTGAGAATTT +CACAAGCATTTTGAACAAGATTCTATGCGGTTATGAGGATATTAATGTGAGCGATCCTGAACGTGAAGCC +TGTTTACATGATGAGACACCAGGATCACCAGTCTATGTTCTTAATGAGGTCGTATTACAGCTAATTGAGC +TCGAGAAATACCAGAAGTGCTCAACTCATGGAAATGAATCCCTCTCATCATCTGTCAACATTAAAGAGGA +GTCGCAGGTAACTATACCCACACTGTTCGGAACCAACAACAACAACCAATCTAGTATCAAGCATTCGCCA +GTATTGAAGCCATCACATAAATTCAATTCTGAAATATTTACCACACAGGCTAGCATCTGTTTTAACAAGC +TTGAGACTTTTTTGAGTTATATGCAATTGGACATGCCGTTCATTAACAGATTCCTTCTTGAACTCAGTAT +GGTAAATCTCCAGGTTCTGAATAAATTGAAGTCAAACCATGAGATCACTGCGGCATTGGAGTCAATCACT +TCACAATTGTTATCTGTTTCATCTAATGGAATCTTCACCACTCCGAGACTTCAAAAAATCGTTGAATCTG +TTAACAAGCTCCAAGCTGTTGATTCGAGTATCTTTACTACACCTCCATTCAAACCAATCAAAGTCGAAGA +GGCTGGTAATTACCATTATGTCAACGAGGATCTTATTTCATCTATTCCAATGTCCTCTTCACATTCTGTT +TCATCACACTCCTCCATATCTCCCATGACTGAGAAGGGAGCTGACGTGCATGTTGTGACTTTGACACATT +TGAGTAATCTTGAAGAAAGAACAATCAGTGAAAGGAGCGCTGTTCAAGGCGATGGCACAGTGATGTTTGT +TCAATCTGGGGAACAATTTGAAGGAGATGAGATCAGAGACATTCTAGAAGGTGTTGACTGGAAGAAGGAG +ACAACAGAGGATGTTTTGAAAAAGATGGGTGTCCGGATATAGTATTTATTGTATTTATGACGTTATTTAT +GGATTATATGATGTGTAGAGATTGATATGCGATTTAGAAATGATTTAGCCGCCCGTTATGTACAACAACG +CATTGAGAATGCATACAGTCGATACCTAGATGTATGGATCGGCACATGGTAGATACTTTCACAAAAACTA +CGTATTCATCGTTCTTTTTTTTGACTTCAGAATGTGGAATAACCTGACTATGTGTAATGTTATAGCAGAT +TAGTATCTGACGTGCTAGCCTACTTTAGGACTCTCACTGATGCTTATTGTATAAAAAAACCATGTAATTT +CTTTTAGGGGGAACATCTCTTTATATACTTATCATGATGGCACCATACAAGAGAGAGAGGTGTTTTGGGG +TTGGAATCACATAGCTTCTTGAATGACTAATCGCTTATGCTGCATTGCATCATTCAAAAAATCACAGACG +TAATTTAAAGAGTCTCTAAGAAATATTACAATCACCAATCACACATCATTTTAGATAAAGGAAACGCCTG +ATGAATCCAGATCTTGAAGTTGTGCAGAACATTTCATACATTTTGAACAATGTGGGACCGTCAAATAATG +AATTTATGCAGCAACTCCTCTCCAGATCAACTATCATTGAATATTTATCACTCCAACAAAAGATCAAGGT +ATATCTCTTCTCACTGGGCCTATTGGGACTCCTTCTTTTTGGGATAACACCAGTTATTTGCAGTTGGCTT +CCTAATGGAAGAAAAGGCAACAAGATTATCGCCTACTGGAGCTTGAATATTGTCTTTGTTTCTCTTCTCT +TCCCTTATCTGAAGTTGATGGGCACGAACAGCGAGCAATTGCAATTGCCATGTGATTTCATAATCCCAGA +AACTAGAGGCATTTCGACAGAATTGGACCAACTATTTAAGTCTATACGTCTTTCTGGATTCAACATTGAC +CGGTTATGTTTCAATCGGTTTGATAATGGATTGCTGACAGATGGGTTCCAATTAGAGATTTCGATAGGTA +CATTTAAAGCGAGGAAGCTTGGAAAACAAGAACAGCAACGAGGTGTTGGAAAAATTATCGGTGATTACAA +GTTTTGCTTAGATTTCGAAAGTGTCGATTTCCAGATCGCTCAACTATACATGCCCAAGATATCTACTTAA +TCATCGCAGTCGCGAGTTGAGCATTTTACTCAACTCGTTGCAACTCTACCTGAATTATCTCTTTTTGGGA +GGACACTACCATAATGTACTACCATAATGTACTACCATAATGTACTACCATTATTGTTACCGGACTCTCC +TCTATTGCGAGTATTACTGACTGCCATTTTATTGCCACTCTCTAAAAATCCTTAGTACCTGTACTACTGA +TTACCCTTCTATTACCACTAATTATTAGCACTTTCCAAAAAATCCCTATTCACCACTACTGATTCCCATT +CTATTACCGGTAATAGGTAATAAATCGTGGTAATAATTTCGCGAATTTTATTATTACCCTCAGCGCTCTA +AGATTTGGTGGAGAAGGCCCAAAAAAGATGTACGGCTGTTTAGTTCCCCCTAAACGGATAAGATCGGGAA +AAACAAACGGGAAAAAACGGGAGGAGAAAGAAAAAAGGGACGATAACGGATGTGTACAGCCGTACCTTAC +TGATAAAGCCAATGATAATTCAACTTTCAAACAGATATACACTCTATAAATAATCATAAACCAATTCTCA +TACGCTTCAGCTCCTCTACAACGAGGAAAATGATGATGGTATTTGGTCCAAGTCTCACAAAACTTGGCAC +CCAACCTCTAAACATGAAGCTGGGACCTTCGTCCTTCAAAGCTTTAGTCAAAATGTGCACCACGGAATGG +TTTGTTCCGGATGCATTCATGATTCTTGTTTTGAGCACATCAGCAGGTGAGCAAATTGTAGTTGCGGCCA +GTCCAGCAAGCAGAGAAGAACTGAAAAATGTGAAGAAGGACTCCTTTTTCATTCCGAAGGAGATGAGCAT +TTCCTTGGCCAAATCGTAAGTGACAACTTGGGATGAAGTCATGAGAACACCTCTCACCAAGTTTGTAGAC +AAGCCTCTAAATAGGGCACTAGGACCTTCTTCAGTCACAATTCGGGCCACACCATCAAACGCATTCTTGT +AGTTTCTTCTTTGTTCGATCGGTAAACCAGTGTCATTCTGCATTCTGATATTCACAATGTCAGATGGATT +ACCAACGATCCCACCAACAGCTCCACTAACCATAGAGATGGGCAATAGATAAGCCATTGAACTCATCTTA +TCGGCTGGAACGAAACTCTCTTTCAAGTACTCATAGGCGCCGAATCTGGTCAGTGAGTAGGTAGCTTGTC +TCAATAAGCCTGCAGAAAGACCAGAGTAGATACCTGTAATCCCATCTGCTTTCAGGATCGAGAACGCCAT +CTGAAATAGCGATGGCTTGGGTAAAGGAGCGGTCTGTAGACGCACCTTAGCCAAATCTAGAGGATGGGTG +CAGAAGCAGGCTACAGCACCTCCGAAACCACCATACCAAAAGGGCCACTTGGTGGTTGGGGTCTTGGTGG +CCGTTATGGTTTTAGCGTTGGCGGATGCTGCAGGTTGCAGTGCGATTGGAGCCCCAGTGGCGGTGCTATG +GGTAGTAGCAGTGACTGACGACATGGCTGACCCGTTGCGATCGAGTTGACTTCCTGGAACTGGTGTGGTT +GGGTTTCAATTGAGATAGGAATTTTTTTTTTTTTTCTCTTTGGTATTTTTTCTTTTTATTTTTGAGATCC +AATAGTAGTAAACCCAAGCGACAAGCGAAATAGTATATGTAAAAACAATAAAGAGAACTCTTAAAGCACT +GCAGACTATGTGTCTTCTATGTTAAAACTATAACAATAAAACTATAAATGCGATAGACAGGTTGGTTTCA +GAATAATCAGGTTTTAGGATTCTACTGGTCTTCGACTTTGCACTAATAGCTTCAAATCTTCAAAAACTGT +TCGTTGTTATCCAAACTCTACCTCATCGATATCTCTTAGTCATTTTTCTACTAGAGTTCTTTAATGATGA +TCTTTAATAAAGGGTCATATGGAACAGTCCCTGTAGATTCTCTTCATGAATTTCGTGGGGATATGCTAAT +AATTGCTTATATGGCAATTGCAAAGTCTATACTTTTAAATTCTATTAAATCAGAGTGTCGCATATGAACT +ATGATTGATTTTTAAAGCCGACGGTATATCCGTTTACATTTGATGCGTGCGTGTGTACATTGGGTAGTTA +CCCTTATAAGCTCAAAATTTCCAGGTAGCTTGGGCTAAAAAGCACTTCTTCTATAGTTATAACATTTATA +CTGCTAGCACTAACTTCTTCTAATATTATTAGGGATATTAGACATGTTTGTAATACTATTGTTGGAGCTT +TGTAATGGCTGACAAAGGCATTATGGTAGGCTTAATTTCAACATAAAACGTGCATTTATTTAGCCTGCAT +GGGAATTAGGAAGTAAAAAAGATATGGATCTCTAAGTGAGTTGATAGTTTCTTATGCGTTAGGTGTATGA +GTGAGCTTTATGCCTGGGTCTGAAATTGCCAAATTGAAACCGTAATTTGAGTATCTGAGGCATCATGGTT +CGCACCGTTTTGTAGTGGGGATAAGAAAGATGGTCCGGGTAAGTTCGATAGTTAGAAAAGAGGAGGGTGA +CGAGGAGACGACCTGATATCACAATGTGCGCTTTGGAATTGGAGTAGCCCATGACGAGGGAGAATTTATC +CTTGGTGAAAACTTAAAACGTCTCAACTCCCGATAATCGATATTCTTCGACACGGAGAGTTCAGGTAAAC +TACTCTATGTTGTTGAATCAAGGATAGTTGACTTTGCTGAATTTGTGGAGGGAGATCTTGATAACTCCAA +ATCAGTAGGAATCAAAGATTAAGAATCATAGCTATAGTATACAGAGTTTCAGAATTCGAGTAAGTTCAAC +TTGAATAGACTTTCAGAAACCAACCAGTGGGTAGGAAATCTCAAAGTAAAAGATAAGCCAAAAAAAAAAA +AGTACATTTTCAAAGTCCAACTGCGGACAAATCCGGGAGGGGAAAGAAGGAACCCCACTTAACGGTTCTC +TTGATAAAGACAAATGTAAGACGAGTATTAATGACGAAATCGATATTTCCACTAACCAAAATCGTTTTGA +CCTAGTTACTTTAGACCCAAATTAAAATTTACTTACTCAAGGCCACCTGAATTGCAAACCTAACGGGTGG +GGACTTTCCAAGCTCGCCAAACTCCCAAACAAGCGTCATTGTGTAGTTTATGGTCGATTTATGTCTACTT +TCAAGCTTCGTTATCTCACGCTGGACTCCTTCGAACTTTACTATTGCTCCGCGGTGACCCAAGGCATTAG +AGGATTCTATCCCCTGCGTAGTTGTATGTTAAACACTGCTTCGATAATGTTTGGGACATTCCAAAAAAGG +AGCGTTACCAAACGCATCAATAATTGGAATAGAGGAGAAATCACTGTCTGAAGATTTCTCAGTCACATAG +GGAGGTGCGAAATTTGAAGCGCCCATACTGGTAGTATCATGAGACAACTTACCATCTGGGACCCTTTCAA +TCTTGTCAACTAATCCAAAAACATACTTCTTTAGAGTCGAAGGATCGTTGTATGACCTTCTGATACGCTC +TCTCCATCTATCTGCATCTGCTTTCATTGTTAAAGTGTAGGAGAGGCTCATCTTGCCATGGTCCAAACCA +GGTATCTCGGTGATCATATCGGAGACTCCAAATTCTGTCTTTTCAGTGTTCACATGAGAGAGGGACTCTA +ATAGCGTTTGGATAACTTGTTCAATAGGTTCCAGGAACCCCTCCGGGATTTCATCCAACCTATTTTGAAG +ATCCTCCAAACTCATTCTCGTGTTCTCCAGCTTGAAGACAGTTTCACGATAGGCAACATCTTTAACCATC +AGATGCCTATGATGACGAATTCTGACCCATGCCCAGCGATAATGGAATAGAATTGCACTACAAAGCTTAG +TCTTTGAAACCAGGTCCAATGGAATTTCCTTCACCTCAATCGAAAGAGCTGTCAAAAGCTCTTTTAGAAA +ACGGACAAGCGCAAGGTTGTCTTGCTCAAACTGACAAGCAAACGAATGCTCACCGCCGATCTTAATAGTG +CCAGACTTGTAGTTCTTTAAATCATAGCGGTCTCCGTATCCTTTGTATTCATGTGGCTTGATCTGAATGA +AAATAAAGCTTTTTCCTGCTAGACGGAATTGAAGCAGGGCTGCACGTGTAAGTGGCTTGGGAGCACAGAG +AACAGTCACAACAGGAGTGCCAGAAATTGCTCCCTGAAATTTGGATATACCTGACAACTCTCTGACTTCC +TTAGGGGAAATAAGCCTCTTGTAATTCTTGCACTGAACCACAAATTTCACACGATCATGATTTTCATGGC +ACAGTGGACTCATGACACGGGCCAATTCTCGGGCAAGGACTGGATTGGCTTGAGGTTTCGCAAAATCAGA +CATATTCCAAAAACCTATCAGGTCAGCACCATTGTCCCTGGTCCCACCAACGTTCTTCAATTTGAGATTT +AGGGCATCCATCAAAGTCATTCTCACAGAAAGTTCATAGAAAGTACCTCGAATATGCGCACTATTATCCA +AATCTTTACCTCTGGCCTTCATATCATCGAGAAAAGCCTGTAGAGTTATACTCGTTGACGTCAGGCTAGA +CGTGGACATAGTCTGCTTGATGATTGTGGATTTGAATCGGGCTATCCATTTGCAAGTGCAATTCATCATT +GATTGAATGCACTTGGCTCTCTGGATTCTTGTATGAGCCAGCATAGCCTTTTAATGCAATGAACCACTAC +AGCAGAGACTTAGTGGGACTTGTTCTCTTTTAATATCAGCATCGCTCCACAAAACATTGTTTCATTGTCT +CATATCGAATTATCAAGTTTTGTGATAATATATCGATATTGTTTCAGTTTGTTTTCATATCCTTTTTTTG +AAATAATAGTTTAAGGAGGTAGGTACATTGGACAGGTGCTTTTTTAAAGGTACACGTTTTCTCTTTGAAG +ATTGGTGATTCAAAATGCTCCCACTAAATTTCGATTAAAGTAACTAATCAGTTCTGGAGCGGTTGCTCTT +ACTGTCTCAGCTGTTGGCTGGGAAGAGAAGTTTTATTTTTGTCAGACAATACAAACAAGTTAACAATGAA +ATAGGATACTGCATTATAATATGAAACAGTGAGAATACAACTCACGTTTGGTGCGTGCCTCTTGAAAACA +TCTTTGAGCTAATATATAGCTGCTGTGGCGAGCTACAACTACCAGCCGTCCAACCTGCCAACTGATAGGT +TCCAAGGTAGCAATTAAGATGTAATAGGTGCATATATTTAGCTGATGATTGTGAATACCACGATCACATA +CATACACCCTTCTGGGAGATTTAAGGTACTTTGTCTGGCTCTGCCCCATAAATATACGTTGGTCGTAGAT +TGCTGCTGATTAAAATCTATCACGTGATCTTTATCAGTGGCCTCTGGGCGCGTTTATTCGTGGGATAGGC +GGGTAACTTTAGAATGAAAGTCGTAGTATTTTTTTTTAAGACGCGTTTCTAACGCGTACAGTCCTAGTCG +CACAAACAAAATAGGGTACAGACAGCCAAAGAGTTCGCTTTGCTAAGAATTTTACGTGAAATGGATAGCT +ATATGACAAGTGAAAACATCCAAAAGCTAATATCTGATACCTGGAACTCCCAAACAGGATCAGATTGATT +TTGGAAGCAGAGGGGTTTAAGAAGATTTGAATTCAAAAGAGATCACTATCCTGAGGTGCAATGCAGACTT +AGAGGCGGTAGGTTTCCCACACGTGAGACTCTGACGATCACTCCATACCCATTGAAAGGGGAACTGATCA +CTGAGCCTACTCTCATTGAAGAAGCCGAAGTAAGCAGTCTTATAGACAGCGAAGATTGACTTATCAATTA +TGCTATGTCTATTACGGCAAACGTAAAATTGAAGCCCGTATACCAAATGCTGGTTGATATACCGGTAGAT +CATCGCAATGTGATGATTCTGGATAATTACTCCGCATGGATTATTTTGGCTGTTCCGTTCGGGACCTGAT +AATACCAAAGAGGATACTATGCTGCTTCAATACAAAAGGAAGCCTTTTCGGCATGGATTTGTGGATAGCA +CTTTTGACCACTGGGCTTTTAGATTTTGGTTTCCAAAAGTCGAAGAGCATGTCAAACCTGAGGAAGGAAT +CGGACGTGTTGATTTGTCAGCGGTTATGTAACTTGACACAAAAATCACACTTTACCACCAACAGCTCCAC +GAGTTTTAACGACACCGTCGATCTGCGTGCACCATGGCTCGAATCGCCGTCAACTCTACCTGTATCAACA +TTTACAACAGTATGCGTCTACAATTCCTATTTCTCCACTTGAAACACAGATTTGTATTCGAGTTGTGAAA +TTTATTTCAAGAATAAGGAAATCTTGACCGCTGGAGCAGTACCTCATAGCTTAGAGGGCGAAATCAAGAG +ATTTAAGATTCAATTTATCAGGGTTAATAGGAGATTAACTTGGAGGCAAAACCCGAAACAGAATCATAGT +CCAAGGCATGACCGCGACCAAGAAAGAAAACCAGTTAATGAGACCTCCAGTAGCATCCTTGAATCTTCAC +ATGTTCTAAAAGCACCAGTTCATCTACACAGAGATAGGTTTCAATCGATTTGCCTTAGCTCTCTCCTAGA +TCCAAAAATAACTGAAACAAGAAAACTTTCCAACAAGCAACCGATTCCAAAATATTAGACTCTGAAGGCA +ATGGTGCTAGCTCAACGCGGTGGAACACCCTCATGTTGAAATGGAGTGTTTCCGGCGCTGTATTACACAA +GAAGACCTCGAAGCGCAGAACCATATCATGGCTAGGATGTGCCTGAAAGCTGGTAAACCTAACCTTTCGG +GACTAAAGATCCTGCGTTCTGTCACCTACTGTTGGTATTGATTCATACCACAACAAATAGCTGAATGACT +AATTTGAGCCAAAGCCAACCAACTCCACGGGTCATGTTTGTACTTTTTGTGCTTATATGGTACGTTAGTT +AAATTGCTACGTACGAGTAGGTATTTCTCAGCAGGATTTTGACGCTCGTTACCCTCATTACAGGATACAA +AGTGTGGAAGTAATTGCTAATATTAGTGTTGAATCCTGCATACGCAAAGAAGCCTTTCCTATTCGTCCTT +ATTCTTCGTACCCACCAAAGCCATGGATTGTCTGAAGTTTGCCCTGACCTAGTCAAAAGCACCCGTACTA +TACCTGAGGTGATTAATTGCATATAACAGGGATCGATGAACATGCTATCATTTGACGTTTTATCCGGAGT +ATGTTCAAAATAAGGACTTGGTTTAATTTAGTAAAGGTTCAGTGCTCGATCATGGGGACTTTTGTTGGTA +TACATGCGCCTTGTATTAATCGGCAGACGAGAGGTGAAACAGTGTCAAATCGGAGTGGCAGTAATTTTAT +GCCTCAGATACGAGAGAAGCGACATGGATCATGCCGAATGGAGTTTGCTAGTGAATAGCATAATAGTCCC +ATGCGTATATCTTCTAAAAAGCCGCGGATCAGAACGAATTGAATTCTTGTCAGCTGCTAATGCCCTGAAA +AATGAGACGAACATCACCGCTGTGGAAGGAGGAACCACCACATGCGTGTTTGAATAAATCGAGATTCGGC +TGCTAGTTTTAAGCTGGCCCCTATAGTTCAAATCCTTCCAAGTTGTGAACTCTTTCCGAATGTTTGGCAT +TACCAGGAACGCATTGCTAATAATAGGCAATTTAGAGCAAAGAATCTTCAACAATTAGAGGAGTACATGA +GGACCAACTGCATAGAGGAATTTGGATAACCCATCAATATTCGTGTAAATATTCCGGAGCTACATGATTT +GTCCACATCTTTTCAACTAGCACAGCTGCTTATGAGTCGTTATCGCAAACCAGAACACAGCAGGCAGAAA +GTTTTACTTTGCAAGAATAGTGCCAGCAAGAAACATGAATGGCTCTTGCTGCCGCGCTATAATACTAACA +AAATACTTTTAGAGAATGTCAAGAGGTCAAGATCCTTCTGATTGCCAAGCTAGATAAGAAATCCTATAAC +CCCATAGGAGTGATAATAGAAGAATTTGATCTGCCTACTGAATGTGAGATGAGCAATATTAAAGTTAAAG +GAAGTTAAAAGCCGATGAATTAACAGCGAGTGTGGCATAATGTGGAGGATAAGCTGAGAGTCAAGATACA +GAGACAGTCACGCAGATGCTTTCCAATTTGAACCAACATGACTTGTTTTGTGACTAACTAACATTCGCCC +CCGGGGTGCTCAAGTGATATCTTCTCAGGTAATAGGATACCGGTGGACTAAAGATTGAGTGGTTGTTCTC +ATATGGAGTACAAGAATCACACAGATCTCGAGGACCACAGATCTCGCGGCTCTTATAAGTTTTCTTTAAA +TGCAGTAAATTATTTCGCTTTTATAATCAGGACAGCCAAAGTGGTGGACATAATTCTGATTACTGAGATC +GATATGCCTGTGCTGCTACAAGTGCAAGCAGTAGCTAGAGAAGTTGCGTGATTGCTGTTCAGAATTCGTA +TTTTGTCCTATGCATTGCTCATAGAACCAGCCTTCAAAGGTTAGTATTCCAGTTATATAAACAAGCGGAA +ATTGCCAATTTGGGAAGGGCTAACATAAAAGATGGTCAATAGTTTATCGATTGTCGTCGAAAGTGTCTTC +ATTTTTTCAACTGATAAATGTAAAGGCTGCCATAATATTACATGCCATACTGCAACTGAATTTTGGTGGA +AGAAATCAAGATCGGAGAAACCTTACAAGCTACAGTTGTCTGAGTTTAGCACACAATGCCGTAAAAGTTT +CTGATCACCTATGAATCCATAATGCAGTAAACAAAAAAACAAGAGAGTACTGACCGCTTGAAATATAAAT +GCAACTTCAAATTCTCCATTGTCTTTTAGTGGGGGCTTACAGATCAAAGTTTAATATTTTGCTATTGTAG +ATTGTTCATATGTAATCCTTAAAATCTTCTAGCGTTATGTTCTGAATCTTATCTTCGTAAAACAAAGGTT +CAAAATAATTTGTTTTGTCAAAGCCACTGAGACACCAGAGATTATTTTTTATATAGGCATCATCCTTTGA +AGACCTTGGCCCACGCTTCTTATAAGTTGTTCCTGAAGTGTGTCCCTTGGTCAGTCTAAACTTACTAACA +AGATTAATACTCACATTTGAAACAACAGTTGAGTGTCTAAAAGAGCTAGTTGATAATTTAACGTAGGTGC +CCATCATGTGAGGACTTGTGTTGACAAACCCAGATTTTTTCTTCAAAGAAGATAACGGGGCACATTTGCT +TATCCGCCCAGTCATAGTTCTCGATTGCTCGAGTACTGGTGCTAAGCTTTTAATTGTCTTGGATCTGACC +TTAGGTTGATAATGGTAAAGTGGATAATGCTCTTCGTGCCAACTTTCTTCAAGAAGGGATAAGTACTTGA +AATATTTTTGAAATGGTGAAGAAGAAGTTTTCCAAATCTAAGCATGTTAGTAACTTTTAGACTCCGTGCA +GGAGAGGTGTTTCAATTACATACTATCGATATTATCTTAGAAACGTCATCATTGGCAATCGTATCGTTGC +AGTTCGCAAGGACTATCTTATTCAAAATCATTCTTGCCACTATGAACTGGTTTCTAGGACGAGCAACATT +CGAATATTGCTTGCTCGAAGTAGAAGGTGAATCTTCGAATGTTAAGGTAAACTTCTGGCCACTAGAAGGC +CCCTTAGGAATAGAAACAAATGGGAGAATACTTTTCTTAATTCGCAAATCAGAGTCATTGCCAGCACTGG +CCTTGAAATGTGCAATCTTAAGACTTTCCATTATTAGTACCGTACTTATCTTCGACGAGTATATTAGCAG +ATATTTTAAAGTAACAAGTTAAACATAAAAAATCTCACTTCTAGGATGTCTGTTAGTTTTTGAGGCAACA +GTTTCGAGTGTGGTTAAACAATGGTAGGAAAAAAATCTAAAACCTTGATTACGTTATCTTTCTTTGCGAA +CTAGAACTCCTTAACTAAAATCAAAATTTGTAACTTAGATCACTATATCGTAAACTCCTTGTGGGTCTTT +TATTTGACACCTTAAATTTACAAACCTCTTATCATAGCCCGCTACTCAATCAATGTCGTACTCAAGTGAT +GACTTTGATGACTTCTTTTCGCAAAACAGTCTCGCACAGGCTAGGCAACAACTTGCCCTTTTGATAAGGG +ATGTTTCAAAATTGGCTGGAAATATTGTCCCTCCTTCTAGAAACGAGGTTGATGAAGTTAGTGTCACTGA +TCCCTTTAATAGATCAAGAACATTACAAGCTGTTCCTAAGGCCCATAGATCTTATTTGGCGAAGCTCTTG +GATGGTTTTGAAGAATCTTTAATTCGAGTTTTAGTATGTAAACTCCCATTGATCCAACTAAGCTTTACTA +ACAACTTCTCAAGTCTCTTCAGGGCATGAACCAGAAACTTATCACAGGTCAGTATCAAAGGCTTTTATTT +AACACATTGGCAGACAAGTACGGTTCTGAGGTCCTAGAACTGATTGACTACAGTGTTGAAAAACCATATT +TTCTTAGCAAGTCTTACAGGGTTAAGTCTTCAACTATTTCTGTGAACGAAAGAAGCGCAACAGAATCTAA +CTGTTCAGACATATCTCCATCTTCACATTCTCCTCCGGAAGCATCATCTATGAATACTTCTAATGTCTGT +TCTGTTTCTGAAGCAGTTATGGCCTCACCAGACAGTAATAACAATATTCAACAAAAGAAGCGAAGAAGAC +ATATTTCCAAGGAAGCTCGCAGCCTTTTGGAGAAAGCATTTGAAACTACTAAAAATCCAACCAGAAAGGA +AAGAGCAATAATCGCAGAGAAATCCGGTATGACAGCACTTCAAGTAAGAGTGTGGTTCACCAACAAGCGT +ATGAGAACCAAGACCAGAATATAAGTTCATGTTATTGCCTCTGATTTGTAGGGCGCGTTTCAAATGTAAT +TCATAGCCAAACATCATGTCTTTTGTGACTCTCACTAGCCATCAATAGCATCTCCTTACCATTTCCTACT +TTGCCACTGTTATCATCGATTCTGTTTACCTTAATAGCTTAGAACATTATTGACTTGGGTTTCATCATCT +GGAAAATTTTGCCTATCTCTGAATCATATCTTGATAATAGTTTACCTGCTGGTGAAACACTCGAAGTTAT +GACGAGACTAGTGGGCGTTTGATGTGACAGGTTAAACTCTCACCTTTCTTGTCTTTAAATCCAACCATTA +TATGTATCCCAATGATATCGAAGTGGAGCTAGGTTAACTATCTTGCAAACACCAACAAACCTTCATCTTT +CGTCTATCGTACTATGTATATAATGTTTATTAAATACAAGATACTTCGATCTCGAAGTATCCATTCATAG +ATACCATTACTCGTTCTTGTTAGAGACTTTTGATATGGCTCTAAGTATACTTAGTTTAGTAATCTTCGCT +AGTGGCTTACAATGTGACACTCTAACCATCTGTTTTCATTGGGCACTAATCTTCGTAATGAGCATCTCGA +TTGGGAGACTTCTGAACTTCATCTCCAATATTCCTTTAACTTTTGTTTTAAGAAGCTTTGAACACTATTT +ATTATCTTTATCTGTGTACCATTATTGAATTGAATGGCCTTTGCTGGAAATAGTAAACAGCTACGTTGTT +CGGTTTCCCTCTCCCCCAAATAGTTTTAAGTTAAGCCTGTAAACTGCCCTTCCCGCCCTTCCTATTTGCC +AATCCTCTTACGTTTACTACTTGTAACTTTCTCCGAGTACAATCGAGGCGCGCCCAAGCTTCAAGTTCAA +GAACAAGCCAATTTCACATTATTAGGTTTTTCAACTCTTCTCAAATTCCTTGCGGCTTCCTGACAGTTTA +TTTGGGTTGTTTGAACGCAGCCCAGGTGTTCTGATCTCTCTTCAGAGTTAAATTTAAATTAACACCTATT +TCGTTCAAACGGCTGTACCAAGGCCGTATTGTATAGCTGATTAACATGTAGTAATTGTGGAATTTGGAGC +TGATTGCGAATAATACTGTAGAATAAGTTCTGTTTTTACTGCGCCCCGAGCGGTACAGGAACTACTAATG +CACCCATTAACATCTACATGACCTAATAGTTGATGCTCCTGTCCTTACTGCTCGCCGGTTGCCACTCAAA +CAAACTTCTGTGCCGCCCGGCTTACAGCATACAGTCGTATTTAAGAAAGTTCTACATCTCGTTCGAATCA +CAGAACGTTCGACCAGAATTTGATTTTTGGTGTCGTTAAAAGAACAGGACGCCATCCTTTTTGAGCAGCC +ATTGGGTCATAGAGCTGCATAGGAAATAGTTTGACATTCTACTCGTTAAGATAAACACTAGCTCTGATGC +TGGGTCCAGGTATTCTCACTTCCGGGAATATATGCCCTCGGAAGTTGACCCCAAAGAGGTGATGAGAATT +ACACGTGGAATTGACAATCAGACTAAAAACTACACACATTAACTAGCACCTAAGGGACTGCTCTCATCCT +CAATGGCTTGTAAATTCTCAGCTGGGGATGAACCAGGCTTTTGCAAGGTTATAAAACTGTTATCGAAGCT +GTGATGAATCCTGCGTCGAATCTAATTCTGGATTTGAAAACTTATAGAGGTAGTCAACAAATCCCATTAA +ACAGGTGAAACCCGAATAAACCATTGAGCTTAATATGATGTAAACAAACTCACATATTAAGAATGAAGCC +AAATGTGGAATGTTGTAGCTCCCGTTTCCGGCCTTAATTCTTGCCCTTGATGGTTGGAAATCAGGTCGAA +GGAAGATTGGATGTTATGAAGAGGCAGAAATTCTCCAACGCCCAAGAAGTAACGCTAAGAAGATCTACAG +TGTTTCATTGAAGCTCCGAGAATAATAAAGGTGGCGAATATGAGATCATCAAGAAGATGTCGATTTTAAT +AAAGTCCTTCTATTCAAAGCTTCTCGTCAAGATTAATATAACGCCAGAGAAAGTGAGTCCTTCCCAGCGA +CAACCACAAAGTATAGAATCATTAATTGTACGGTAACAAACTATGAGTGGGGATAGGCAGGATACAGGCG +TAGTTAGCAGAAGGTGTAGAGTTATGATTTCATAAGGAGAAGACTGCATTGATATACGGTGATGTTAAGA +AACGAATGTATCAGATCAGGTTATTAAAAAAGCGGCTATTTTACGGTCAGACTTCAAGAGAGATTGGATG +ATTAGCATCAAACGTAACATTCGTAACATGCGATACAATGCAAAGCATAGGCAGGGCAAGCTACAGGAGC +ACCTCGAGATTATTGTGTTTTCTATATTTTAGTCTTCCCAAATTGTTATAGGGGGCAGAGCATTAATATA +CTTAGAGATACTTGTGAGCGTGATGTGATAGTCGTTACGAATACGAACCAACACGTTTGAAATGTTCCGA +ATGGATTGAAGTTAAAGTGCTGCTGCTACTGCAAGTTTACTACTATTAGCATCTGTCAAAATGGAACTTG +TAAAATAGACGTTGGAAGAATGGACAAACTAAATGGGTAGCATTGAACAATATGTATGGTTATTAGTCGA +AAACTAGAGATTCTATTGATATTTTCAGGATTATTTCCATTAAGAATTTTTTGTAAAGCACTGTTTCGCG +AACGCAGACCTTTTTGAGGCGTGGCTTAACGATTAACCTTCCGGCTTGACGGACACGATCTATTGAAGAG +TGGTATGAAATTAACCAGCTGTATATACATATATTCAGTTTTTCACGTTGACAAACAATGCCTAGGAAAG +AAGTGAAAGCAAAGTGGACAAGCAACCCGAAGAAGCCATACAGTGCTGGATTTAAACTCTTCTCTCTTCC +TGGACTGAAACATCGCAATAATGCCCTTGAAAGTAAGAACCCGTCAAAGTGTACCTCCCCTTCGAATTTT +CAGTGTCTTACTTTTGCCGCTACGTCCCTACCAAAGATACCAAAGCTTTCAGCCGATTATGAAAGATTTA +TTGAAGGACTAAAGGATCATAAAGCTGGTCAAGATAGTAGTACGTACATCCGCCAACATTTGAGGAGAAA +GAGAGGGCCCTCAAGAAAGCGGACAAAGTTGAATGGTTTTATGGCCTATCGATCCTTCTTCTCTAGGAAC +GTTGCAACAGATCAACAAGGAGTCCTATCTTCTCAATTGGCGAAAGAATGGGAGAGGAATCCAGAAATCC +AACTTGTATGGAAGAGATATTCCACAGAGTACAATTTGTCTGTTTTGAGTGATCGCCTTCCCTTTTTCGA +TTGGCTTTTGCTTGTAACATCAGAGCCTACTTGTATCTCTGAAAACATTTATGACAACAAGGATCCAGGT +ACATTCTCTAGAATTCAAGATGTTTTCAATACAAATGAGGCAGATGAGAACCACCAGTTTTTAAAGATGA +GTTTTGATGTATCTGTGATCGATCCAAGAATAGGACAAGCATTCGAGCATTCTATATAGTTTATGCTAAA +CCGATAATTGAATCGAAGCTTATAACAATTGAATTACTGTTGTATTACTTTTATACATTGACTGATCTAT +TGTAACCAAACAGTTAGTTCGAGAGTTGATACAGGAACGTCAATGTAGGATGATGCAAATGGGTTAGCAT +CTAGTAATGTTCTACCAACGCCATCGTCTCCTCAGCCTGTGAACAGTATTATTAGAACCTTAAGCTACAC +CTATCCTTGATGAAACTCAGTTAATGTCACTTCCAAAGTTTGAGTGCTTCTTTATAACAGCAGGAACGGC +TCCTCGCTTTCCCTAAGTCGATAAGCTATTTCCCCACTTTGATTATCTTTTTGAAACAAACAGTTACGGC +GGTTATTGTTATTTTTAATCTGAAACAGAAGCTATCAGTTATGTGAAAGAATACGCCATCTATTACAGTT +GAAGTTGACCTGAACATTATAAGGAATAGGGATAACCTACATTCTTACTATTAAGTATTGGAAAAACGCA +CCTAAGATCTTAGTTTTTCTAGCGATTATATGTGTATATATATTCGTTTCTTTAGCTGTTGCATGATCAG +TTGTTTCATCTCTATCACGTGATTTATGTTCATGCTGTCAAGGGATTTTGAGCAGTCTTGAAACAAACTT +TCTCTAGCTCCCTGTTATTCTATTACTACAAGTTCACTTCCTGCCTCCAAACAATGTCTGAAGATCAGTT +TAGCAGATGTCCAATAACTTCATTACTCTCAGACACTGATAATATACAACAGGGGGATACTGCAGAGTAT +GTTCTTTTTGAAACTCTTGCAATGCAGATTTGGTACTAACAATTAAATAAGTTCACGCTATTTACTATCC +TCGAGCTCGAAACATATCCTAGAAAGCTGCTCACTTATTAGCTCCATTGATGTTGGCACACTTTCTTCGA +ACATCCTAGCCTCACATATGGTAAGTTTTACTTCTATGTCAAGCGATCAAACTAACACACTAAGGAGTTG +GATTTACTTCAGAAAAGACATTCAAATACTACAGCAGAGGATCAGTCACAGTTTCAAGAAATTCAAAAAG +ACTTATTGAAATCTCAGCTTTTGCTCTCCGAGAAGGCCATCTTAGTCGAAGCCCTTGAGCGGTTTTCAAC +TTCGACAGAATCTCCTAACAATCCTGACCCTTTTAGATTCAATATTATTACGCAGAAGATGATTAGAGAA +GATGGAAGTATTATTTCTAGAAAGAAAAGATTAGCAAGGCAGAAAGTTGCCATTCTTGAGGATTGGTATG +TTTTCTCGCACAGTACCCTATTCAAATTTACAATTAACATAGTTTAGGTATTATAGTCATCCGGAGAAGC +CCAACTTGAATGAATCAATGCTTCTGTCTTTGGTCAAAAAGACAGGTCTCACTAAAGGCCAAATTCGCAA +TTGGTATGTGTAAGATTGTCAGAGATCATAGCATGAACTAACTTCTATACTTAGGATTTCCAATAAGAGG +TATGTGTGCACTTCAAAGTTTGTAATGAATCGGTACAGTACTAACAACATTGATAGAAGAAAGGAACGAG +AGGACAAAATAGGGGATTCAATTTCATGTCTAGTGTCGGGACTTGTTAAAAACGATTGTCCCTAAGCCTA +TCACTCCAATATGTGAACACTTCTCCTTGCACAAATTATTAGGAAATTCTGAAATTGTTAACATTGCATC +AGAACTTTTGAAACCTTCAGGATGGCAGCTTGAAAGATACAAGCTTTGAGAATCACTATTTGATCTATTT +CATTTGCTTTCTCCCGACTGTAGTTTTCTGTTGTTACAAATTTTGTCCCTTTCATGAAAGAATTCAACTC +AACCAATCCTTTTTCAGTTATCACTTTCATGATATTCCACTCCATGATAGGACGTGGTGAATGTCTTTTA +ACATATAAGCTTTGAGTCTTCATTTCAGCTTCCTGCAAAAATTTTAATGGGGTGACACCACTTTCTCTAA +ACTTGCAACACTGTAATTTTGCCTGAGCGACCTCTACGCAGTCTCCATATACTTCAATGGAGTGAGTGAA +CGCCACCCGTGGTTGAAGAAGTGATCTCACATACTGAACGTATTTTCCTCCATTTTTTTTCATGTCAGGT +ACTTCAAATATTACCCTTCTCTAAGACCATTAGTAATCGTAGCCTTCGCCAACTATATCTCTCCTCGTCA +TCAACAGTCAAATTCAACATTCTTGAATCCAGCTCCTTTATAGGAACTCTGTAAACCATTTATCTGCAAG +CAAAATTAATCTTGGTGGCATCTGTCAAATTCCCCTAGGTTCATTGCCTGGATAAACAGTGATTTTAATC +TGATTCGTTGTGATTCTTCTCATGGAAGAGTTTTCACCTCCAAACTTTCCGAGGTGGGATGATTTTTCGA +TCAAGAAGAGATCAGTTACTGATGCTGAGTGACATTTTATTGCTGGATTTATCAGATTCCCTAGCCACTT +GTGTCTGGAATTAATCAACGGAGTTCGTTCACTTTAGAGACTCTTCTGGATAGTCCTCCAACCGCCACTG +TTGCTACTCTTCTCGAATTATTGGTCTAGTAAGTGTTGTTCAAAGACTCGACATTACTGGCGAATATAGA +AGGCGCCCAGAAGTCCACACTCAATAATAAGCGTTAGTGTCGGACCTAACGGTCTGACCTAAAGTGCAAT +AGTCTTTTTTAGCTGCCGTATACCTTTGTGGTAGGATCTATCCCTCCCCAGTCTTCGAAAGAGTAGAGTA +CATATGTCTTCCTCACCAAGGTCCTGAGCTATGCGTTAAAGGATTATGATATCCTTAGTAGTGTTTGGCA +CATGCAACTCATCCAAGGACGGTCCACATCGCATGGTCGTACTGGCAATTGCATTAAAACCACTCAAGAA +GGGACTCTCCCTGCCTGGCGATTGAGGTTGTGATAGCAACGTTAAAGGACGCCCATCCGTATCTATGGAA +TTTGTGCCCATAACCCAGATTCGTTTTCACTCACTAATTTAGCCCTAAATGGTGGAACGAAGATGTGCAA +CATGAACATTATCCTTTTTCCGTTCAGTCTTATAAAGGCGAACTTGAATGGCTTGCTTCTGAGCAATCAG +TTAATTGAAACAGATCTTAGATGAATCTTATCAGCAGTTTCACATTATTTAAAATTGCTTAGACCTAACC +TAGATCTACCACCGAGAGTTTTAAGTCTCATTTCATCAGGCACTTTAACCCCGATAGACTCACCTCGAAA +TTAGAGCTGGTTTATCAATGTAGGCTTCTGACTTATCACCCGATCTCAGAATATATCTAACAGGAAACAT +CGCCACACATAATTGATACCCTAAGTCCATTGATGCAAAGTTATAATCTATCCGACACCCTTCGAGGTCA +TTGGTTTGGTCGCTGGTTTTGTGCAATACCACTACCATCACGGCCAATTGCTGTTTATCGATTCTTAATC +GAACTCTTTATCATGACGCGTCATCTTCCATATCGCAGTCTATGGCGCCACCTTTTCTGCAACGAGGCAC +GCTAGTTCGGCTAATCTAAGTCCCAGAATGCTCCCTCCTTCGTTTTGCCATTGCTTCCAATCAGAGCATT +CTTTCTATGTGTGTCATCAGTATCTCCAATATGTTCTTCCGGTCAGAAGTAGAGGTCAACGATAAGTTTT +TCTGAAATAGGTCAGAAGGTCAGAGGTTCTATTGCTTACTAATATGCTGGTCAGAAACAGACATTATCTT +CGGCGGATGTTGTGTATCTGTGATTGGAAGTATGTTTGTATTTAAACTGGCAAGTAGAATTTCACTAATA +GAAGTGTCTGCTTAAACTAGGCAGTTTACAGAATAAACAATTTTCTTTACCAAGCGCGTTGGTCGGATTC +TGTAAACGCGCGAAAGGAACCGACGCCAAGATCTGCAATATTTAAGGTGGAACGTCCATCGAGATACCTG +ACTATGAACTGTTCGTGAGTATCCCGATCCCAGCTTCCTCACCAGAAAACTCCTGGGGCTACTTGAGATT +TGGGCCATAAGAATATTTAATATCTTTATTCTTGAACTGTTCATGCTTCTTCTAGGAACTTTCGAATAGC +TCATCACCATAGTAGAATTCTGGGGATCTTTACTTTTGGTGCAGTCCTCTGGTTTCCCATGCCACCTCTC +TGTTGGAGGGCGCAGTGACGAAAAGGGTAATCAATATTCAGCTCTTGCAGTATTCTGCAGTACGTGGGTT +GCCTCAAATAAGGTGCACGCGTAGATGTGAGACAATTGCAGTAGGTCAAATGGAGCTGATGCGGCATATT +GCATTTGGGCTGTACTTCTTTCTCTCAAAAAGTATTTGCCAACGTTGTACGGAATCGAATCGGTCAGATA +TCCCTTTACAGATTATCAAAAAACAAGATGCATCAACCGCTATATTGTTTTTGACGCGTCTTTTAATACT +TTGCTCAATGGTAGGCAGAATTACACACCGTGTATACCTATTGTGTCGTGGCCATCATTTTCATTGTGAG +GAATCTACCTCATGGGAGTTGGCATTTCTGCGATCGTTGTAATTTCATGTGACCCGTTAGTTTCCATCCT +CCACTTTTTTGGGGTCATGAGTATCTTTACCGGTTATACTCAAATAAACACTCAAGTCTTATTCAGGAAA +AAAAAAAAGACTGTTTGTTTGCTCCCTGTGACGCACGAAGCAAGACACAGGGATTGGGTGACTAGAAGAA +CGTATTTTCTCAACAACTCACACACCATATACACTTTATCATTATCCAAGAAAGCAAAATAGGCCTTTAT +CCATCCAGGTACGAAATTAGAATAGCTTTATTTCCTTTTCATGTCTGATTTCTATCTATCTCTATAGAAT +AATAGTCGTGGGTGTCATCTTTCGGGGTAATCTCCACATTTCCCAATGGTCTGTTTATTTGCGAAATCGG +AACTATCGTAAATATCCCAGTGCCTCATTTCAGATTGTCTTTAATGCTGGGCAAACAGTTCAAAATTGCG +TTAAATGAAAATATTGACTGTAGACATAAAGGAACAATCCAAGTTACACAGCCATCTCAGCATCGCATGA +TTTCAAAATCAAAGCACAAATCAACTCAAAGTTTGAAGCCAGTTGCCTCTTTTGCTGACACGACACTCTC +AATTGTATAGCCCGATATGTTGTGATTTCAACCCCAATGATCTTCCAGTTTCGTAATGTGATGGTTTTTT +TGAACATTTCTCCATCCATTTGTGGAGCAGGGAAAATATATACCGCAGCTTGTTTAGCTCATACCTTTTC +ACCAGAAGGGTTCAAAATGATCCAAATTTTTGTTTCCAAAAGTGAATTATTTAAGCTTAAAGATGTGCAC +TCAATTGTTCACTATTTGTGTGTTCTGATTAGCCAAGCACATATCATCCAATCTCAGAGGCTTTGCGCAG +TAGCTCAAACAATTATACACAAAGACGATTTTGATCGACCTACGACTAAGGAGAAATGAAAGAAACAAGG +TGCGCTAGATGTATCAAGACACAACAAACATTAGGGTACGAATGTTAGAGCGAAGTACAGATCCCAAATT +TAAGCAACAAAGTTGATGTCTCAATATCCTCCTACTCATCCAATTTGATCAGAAAAATATTGAACCAATA +GCACAGATGTCTGCATTTGCTAGGGCTTTATCATTTCAACGTTGCCCTTTGTGATATCTCTATATACGCC +AAAAACGATAAGACATTTACATATTTGAAGATTATTTTTGCTCATATCTTTGGTCATTAGTTTGTGGATA +TCTTCAAGAGCTCACTTGCTCTAAATGCAATAGATAGCTTTTCAGTTCGTCTAAGAACAAATTGAAGATT +TCGACATTTGGAAAGTGAACAGTCTGTAACTCTTTATCAACTATGCTGTCAATACTGGGACCAAATTTGC +TGAGCCGGCTATGAGCTATACAAATCTGAAGCAAGATGAAAACTCAACAGAATCATAACTTTAGAAGTTT +TTAAGTACTGGAGAGCGTGAGGAGCGACAAGCTCCTATAGATGATATTCTTGTTTAGCTGCTATTTGAAA +AGGTTGATCTGAATGTCTCCCGGTTCACTTCTAACTTAGTGAGAATTTGAATAAAGAGTAACCTCGGATT +CTACAAAATCAGGGAGCTTTAGAGCATTTTGCAAGCTCATCACAATCTTATTTGCACTCTAAAAGAACAC +ACTGGAAGCACTCAACCGTGCGCAACCTGTCTGAAACCAAAAAAATAGGTGAAGTTTTATTAGAATCACA +TGAATCTTATGACCTATATAGCCTAAGCCAATTAGAGTTGAAAGAAAGGTTGCATCTAAGTGAAGAAAAA +TCCTGCCCACTAACCAAATCTTATATTTCAAGTCAACTGAGGGGAGCAAAAAGTAGCTAACTACATTTTT +TGTTTCCCTTTCGAGAAAGTAACAGCTCAGCCTTGTTCTACATGAAGGTCAATATCAGCCTCTTATCTCG +AAGAAGAGGAAAGATCTAGATGTAGATGATGACGCGTGCTATCCATGCCCTCGTGAAGACCACTCAAGTT +CAAGATTTTGAAATCTTTTTTTGGCACTCCCCGCCAATGAACTCACCAACTCAGAACATACGGAATTTAT +ATATGAATAAATGGAAGTATACATTGCAATCATAAAATTTCTAAAGTAGGCTCAAAGTCTGTAAAGTGAT +AGTTCGTGTTATATATAGGTAGATTGATATTGATCAGAAATAATTTATGCATACGTTTAAACCCCTTCAT +CTGTTTAAGCTAATAGTCCTTTTGGTTTCCAGAAAGACATGCATGGTACAGCATTTCTGTGGAAATTTGG +AATAGCTTATTATGTGTAATCATTTTATGGTATCTGAACCGGTACCCTGCTTTAAGACTTTTTGCTGATG +CTTTTATTATGAACTAACTATGTAGCATTCCCAAAGCCATCTAATATCCAGTATCCAGTATCCAGATGGC +ATCCATGTTGAGGTTCGGAGGTTCGATCCTTGGGTGATCATAGACATTATTGATAGTGCGCAGAGACAAT +GCTTGACGGCGAGCACCTTGAAGATGCATCGATTATTGAAATTAAATGATGGGATTGTTTCTGATGCGCT +TTTTCCGAAACATAAGGTTTAATTCTAAGTGAATTAGATGTAAACGCTTATGATACATGTATTCTTCATG +TAATTTTATAAGCACGTAGACCATAATTTCCTGAATAGTAAATTACCCTTTTAGGAAAGCACCTGCATAC +CTGGATACTGAGTATAACCCAGGTTATTATTATTTCCCTTGAGGGAGTATCTCTATATATACTGAGCTTT +CGAAGAGCTCCACCTAAGCCACATGTGATTCTACTAACAGTATCAAATTTTGATACGTTGTTGACGAATC +GCATACCCATCTCAATTTCAAGCCAATAGAAAAATATTCCGTCGGATTAACCGGATTTCAAGAATTCTAA +AACGCAATATGGAAATGTAATCTGATTACATGAATAAGAAAGCAAGAAATAAGCCATACCCTCATAAGGG +AAATGAAGTTTATATGGTACCTCCTATAATAAGTATATAAAGAGATTCTTCTCATAATCGAATACACTGT +ATAAAATATGCATTAGTAAAAGTCTTGAACCGCCAGATACCTCCCTGTCATAACCACTACTGTGCCTCAT +TGAGTTTAAGTTATTTCAGATTTCCATATAGTGCCTAGGAGTGAAGTAAATATTGCATTTCTGTGACACT +CTGCACGTTTGTTTGTACGGTCGGCATAACGAGTCTATCTTGATAGGCGTTGAAGAACTCACTACATAGC +GTTTTTTAGAAAAGCCTGGGGCTACTATATGTGCAAAGCCCATGATATGGAAATTTTCGCAACTACGGTT +GATTTAATTCATTCAGGTTGGGGAATATTTGAGGTAGTTTTCGGATTAGTTGTTTGAGAGCTTGTTTTGT +TTTTGTTTTGACAACCAAGAACAAGCTGCTACCTGTTCGTGAATCATAGCCTATTGAATTTGATGGATGC +AAGTGTCGGTTGTATGTTCACCAAGAAACTATTTTGTAGAAAGCCGGGGTTCATGGGAAACACTCAGTTA +AGTCATTCTGTCGTTATCAATAGTAGTATCGATATTTTCGGTTAATGGGGTCCTAATTTCTATCTAAAAG +ATAATGAAGTCCAGGATTCTGTTAGAACACAGTCTCCTGAAAATCCCCGATTTGGAAAGTGAAAGCAAAC +ACGTCAAAGCTCCTTCTTTACAGTAGACGAAACAGCCACAAATCAAGAAAGGGGTGCTATAGTTGTGTTT +TAGCGGTCCAAGGATAATCATAGGGTGCACTATCTTTGAGTCAGTGTCCAGAGTGTTTTATCGCTAACTG +TATTCTGTATGTTTTCAGTGACGCCACAACATAAGCAACAAAAGAGAGCTTCATTGTTGAGTTTACTCTG +CATTGACTAAATAAAAAGGAGCCTCCCTCTTATAACTTCCTCAGAACTTTCGAGAAGTACAGCTTCTCAT +GAGCTATAGTGTTTAATGTAACTTTTCAATGTAATGAAGGGGTGCTCGCACTTAAACTCCTGCGAAACTG +GAAGAGTTACCTACCAATTGATCATTTATCGTTTTATATTCATGTAATATAGCTATTACATGTGTAATAA +GTTATTTCATTCTTCTTCGCTACTGTCCCCGTCTCTGTAGCCATACTTTCTAATTTCTCCCAATCTTTTT +CTTGCTGCGATTAGGGCATTCTCCAATTTCAGAATCTCAACTTGTTGCTCCATCTCCACAGTTTTGTTTT +CATGATTTGTTAGTTTTGAATAATCAACATCATTCTCACCACTACCGCCGTTGCTTCTCTGAGTTAAAAT +GTCTTGTACTTGATTAACTAGACTTCTGCAGGCAGATGTGACGTTCTTGGACGCAGTTTCCAAATTGTCT +TGAGTCTTAGACAAAAACGTAGCTTTGACTCTCGAAGCAGCTACCAACTGAACAGTTGAAGCAGCCACCT +CATGTGATGCGACAATTAACTGCTCATGAGATTTATTCCCAGACAGAACACCATCCGCAGTTTCGATCAA +AATCTTAGTGGAAAGGGCAACAGATTTAGCTGCTGAGATAAGGCCTTCTGTCCAACGGTTGTGCTTTTTG +TAGTATTCAGTCTTAGAAATTGAGCCTTTGTTCTTTGCAACAATTTCCTCTTGTGAAGCAATAGCTGCTT +TAATCAAAAGTTCAATGGAATTGGTAACGGCAATCGCAGCGGAAACAATAGCTTTGTTAATCTCAAAGTC +GATTTGTGAGATAGATGGGTCTTGATCTTTCGAGAGTAGCGAAGTAAGCTGGGATGATGCCTTTGAAATG +GAGGCGGCCGCATTCTGCAATTCAGTTTCCACAAGGTCGCCCAAGTTCGCAACGTTGCTGTTCAAAAGAT +TAGATCTTGGGGCTAAGTCCTCAGTCAATTGGGTAAGATCTTGAAGTTTCTCTTGCACTGCCAAATTTCC +GGCCATGACCACATCAGTCTTCTCTTCCACTTCGCCCTCTAAACTATCGGAAAGCAAAGATTTGAAGAAT +TCTTCCGATTCCTCTGCAGTAGCTCTCGCAGTAGCTATGAATTGATCAGCCTCTGCATCTGATTTAACAA +GGCGCACAATACCCTTAGAGTTTAATAAAACGTCAGCAATGGAAGAAGTGAAGTCATTGAGAGAACGGAT +AATCTCAGCGTGATCTCCATTTGGCCCATCAATGATGAAGTCATTAAAAGAGTCACCGAAGTCCATAGCA +GAGGATGACGCTTTCTCGATAATGGAGAGTAAATATTCAGGCGATGAGTTCTGGTTACCAGCTTGCATAG +GAGAGTCCAATTCATATAAGGCGTCTTGTAATCTCTTGAGGCCACTGAGCAAGATAGCATCAACCAAGGA +AGTAAGCTTCGTTAAGTGATCAACCAAAACTTGGTCGATCTGCTCTTCAATAGCTGTTTCATTCTCCTTC +TGAGCTTTGGCTAATTCCACCAGCGCATCGTCCATTGTTTGTTGGAGAATTTCTAACTCTTCATCCTTAT +CCTTTAGTAATTGTTCCAATTCTGGATTGAAAGCCGAAGATTGCTGTCTTAACTGGTTCTCCAAAGCTTC +GATCTCTTTCTTGTGTTGAGCAAAAATAGTGGATAGGTTCGATGATTGGTTTCTCTCGGCATCATCTAAT +TTCAAGGTGAGATCTCTAATTTGCAACTCAAGCTTGTCAATCTCATCACCTTTACCACCCTTAACTTTCT +CCAAGTCCAAACGAGCACGGTCACGTTCACGGATTAGACCAGCCAATTCCACGTTCTTCGATTTCAGGTC +CTTTTCCAATTTCTCACGCTTGTCAATGGCTTCTTGAGCAGATGCGGCTTTTTGTTGGAGTTTCTTGAAC +TTATTTAGCAAGTTCAAGTGTTCCTGTCTCAATTGAGAGTAGAGCTTAGCTAAACTCTCATACTTGTTCT +TCCATAGAGTTATTTGCTCATGCAAGTTTTGAATCAACTCATCCTTGGAGCCAATTTGCTGAGTAGCTGA +TTGAGTGGTAGCTGTAATTTCAGTCTCCAAGGCCTTGACACGCTGATCATATTGCTGTAGTAGAAGTTGG +TCTTGCTCATATTGTCCTCTCAAAGCCAGCAGATCGCGCTCTAACTCAGCTACGCGGCCCTGGGCTTGTG +ACTGGTACTGCTCGCGAAGCAATTGCTCCTGTGCAATACGCTGCTGTTCTTGCTGTTGGACCTGCTGCTG +TTCGAACAAAATTCTTTGTTGTTCAGCATACTGTTGTTGTTGGAGAGCTTGTGCATCACGCTCTTGTTGC +AAACGAATTTGCTCAGCCTCAAAACTAGATTGCTGATTGCTCCAAAAATCGACACCAGTTGGTTGTGCAG +CAATTGGTTGCTGGAACTGGGAGACAGTTGGCTGTTCAGAAATTGGTTCTGACAAATTTGTCTGACGAGA +TTCTGGTCTTGGAGTCTCCTCTTCGACGGATGATTCATCCTTAACCATGAGGTTAGGAGCTGTCTCTGGT +AATCTAGGAATTGTGATCAAAGAAGTCAAGTAGCGAATGGAGGAGCAATCAGCATAAAATTCGAACAAAC +GAGCATGTTGCGACTCGTAGCGCTGCTTCAATGGCTCTAAAGCATCATCAGATCCTGTGGAACGGTAAAT +TGCCCTCAACATCGAGGTGATGAACTTATAGATTCCATAAGATTCAGTAACAAGAGGGACCAATGCTGAA +ATTTTGCATTCAGATCTCTTATCATGTGAAATTGTTGCGAAAATAAGTCTCTGCAGATCGTCAAGTGAGT +CTTGAAGGGACATCAAATCCATGATGGCTTCATAACCTTCATCTGGGTTTTGGACAGTCACCAACGAGAT +ATACTCTTCGTACTCAAAGGTTCCATTGAAACCACGGTGATTCCGATGAAACTGAAGCTTTCTGATCAAG +TAGGTGACATATTCTTTAATCAATCTACCATAGCCCTTGAATCCATCACCTTGTATAGATCTCCCTAAAC +CATCGATCCAGTTCAAGTTCCTGTAACCTTCTGCGAGAGCAGATTGGTGACCTTCTTGAAGAACTTTGTG +GATGGTGATTAGAGCCTTAAATAACTGAACTTCATCATTCTGAATAGGCAAAATCTTTAAAGCGTTCCAG +AACGAGAGTGACGACTTGTGATCCCAGGTGTAAACAATACACGCTCTCACATGCTTTCTTTTTGGGGCAG +TCTCATCAGCAGACGTAGCCTTACGAAGGGCAGTCTGGAGATCAGACTCAGAGATCGTCTTGGACATCAC +AATGGTTGTTGGCTAAGAGAATATTTGTCGTAGTTGTTCGCAAAATAGAACCTCCTATCGTGCCAAAGTT +GACGGACAATTCGGTCGTTCTCAATTTCGGCAATCTTGACCCTCGGAACTTAACACCTGCACACCAATAT +CGAAGGAGGAAAATTACGCGCGAACTCAAGGCTAGTCCAAAAAATTCACGCATTTAGCAATTTCGCGAAT +CAAAGAAAAATTTAAGAAAAATTTAACAGTGTGAGTTATAGAGAAGGACGCTAGACTATTGCATCCACAC +GGTCCATTTCCTGAGCGAAAACGTATATATTGTCACTGATTGGACACAGCCAATTGTTAGACAAACCTTA +TTTCGTTAAGGGATCATCTTTTTTTGGTGCAAGGAGCTATAAATACTCATTGTTTTGGCCTAGATCATGA +GTGGAGCATTAAAGAGAAAGTCAGAGCTACCTGACGAGCTGAAGTCTTCCAAGAAGCCATCGTTAATCAA +GACAAAGCTTAGTGATGACATCCCGAAGACTGCCGACGAGCATGAGAGTGATGGTGAGAATAATGGAGGT +GATGACCACGAAGTTGACGAAGAAGAAGTTGATGTCGAGGAACAACAAAAGTCCTCCTCTTCAAAGCCTC +ATTCGAAGCTTTCATCTCAAGATATACAGATAGCGAGAGAGACCGCAGAGCTCTTCAAATCCAATATCTT +CAAGCTACAAATTGATGAACTGATCAAGGAAGTCAAATTGAAAGACCACAAGATTGCCAAGATTGAAAAG +TTTCTACACAAGCTTTATGAACTGATTCAAGATGTACCTGAACTTCAGGACCAGCCACTAGATCAAGTAG +AGTCATGGTTCAAGAACAAGAAAACTACAGTGCCATTTAATGACCCCAAACCAACTAATGTGAATTACAA +GTTTGACTACAAGAAGCCGGAGAATGTTTCTATCGTGGGGTCATTCGGCCTCAAAACAGCTATCCAATCT +CCACATGGCCTAGCAATTGACGTCAATGTTACTATGCCTACTGAATCATTCCAGGCCAAGGATTATCTGA +ACTATAGGGCCTTGCATAAGCGTTCATTCTACCTCGGATACCTCACAGAGAACTTGAGAGTGATTTTCAA +GAAAGAGGGTCTCGACTTTTTGAAACTATCCTACACATACCTCAACGGAGACATTCTTACACCAGTACTG +AGACTGACGGCTACAGATTCTCAGCTTGATTCGGAGTACAACTTCTACAAGACTAAATTCTCCATCAACG +TCCTGGTGGGCTTCCCATACAGAGTATTTGACCCTAAAAAGCTGCTACCAAATAAGAACTGTATTAGAGT +CCAGAAAGAGGAAGACTTGGAACTACCTCCAACCCCACTTTATAACTCTTCCATCCTCACGATGACATCT +TATGACTACTTCTTGAAATACCTATATAAAGCCAAGAAATCCGCAGAGCAGTTCAAAGAAGCATGTATTT +TAGGAAGGCTATGGCTATCGCAAAGAGGTCTGGGATCGAGTGTTGCACAAGGAGGATTTGGTCATTTTGA +ATTTGCCACGTTGACTGCTGCTCTCCTCAATGGTGGAGGTGCTCAGGGAAACAAAATCTTGCTGCATGGG +TTTTCCTCTTACCAGCTGTTTAAAGGTGTTATCAAATACTTGGCTACTCAGGATTTATCTTCCGCTGGCT +ACCTGCAGTTTTACTCCGACTCTTCAGATCTCAGCAGATATATCTCTGAGGGGTTCAATACTCCAACCAT +TTTTGACAAATCATCCAAGATTAATATTTTGCACAAAGTCACTAGAAACTCATATGCACTGCTAGTGCAA +CATGCAAAAAAGACGTTGGATCTATTGAATGATGTGATTCAGGATAGATTTGATCCGTTGTTTTTGAAGA +ATGCCAATGTTGATCACTTGAAATACGATTACAACATACAGCTCCAAATTGACGCATCTACTGACGATTC +ATTTGGTCCATTCGAAAAGATTACCCACCTAACATATGAGAACTTCATTACCAACAAAATTTCACAACTG +TTGAAATATGGTTTGGGTGAGAGAGTAAAGGGCTTCAATGTAATAATCAAGAATTTAGTGACAGAGTTCG +AAATTTCCAAGCGTAAACCTTCACAGCAGAGCAAGGTGAATGTTCAGATTGGTCTGTTGGCTGACTCCCA +CGAATGTGAGAAACTTGTCACCAAGGGTCCTTTGGAATCAGAAGATAGTGAGCAGTTTAGATCTTTTTGG +GGCTCCAAATGCTCTTTGAGAAGATTTAGAGATGGTTCCATCTCAAACTCTGTTGTGTGGACAACTGATT +ACAAGCAACCAATTATCCTTTCGATTGTCGAATATGTCTTTAAAAGGCATTTGACCGAGGAAATCAAGAT +TGAAAGTTCTGTTTATGACTTTAATGATTTGTTGCCACTGCCAAATCTTCCATCATCAACTAAGCAGTCT +ATCACTTCTACCAGTGCTTCTGTTGCTCTTCGCAAAAGTTTTGATGAGCTCTACAGAATATTAATTAAAT +TGGAACTCCCATTAGCTGTCAAGTCAGTGCTACCTGCTGGCCCAGCTTTGAGATCTACTGCACTTGTGAC +TCCTGTCCCATTTGCTGTTTCATCCAAGGAATTTTTCAATGATTTGATTATTCAGTTCGAATCATCAGTG +AAGTGGCCTGATGAGATCAGTGCATTGGAGAATACTAAGACTGCGTTCTTGATAAAGATTCATGAGATTT +TGACCAAGGAAACTGTTTACAGAAGCTTTATCAACAAAGACGAGGACTCCATTCCCTACAACTTCGACAT +TACAACGTTGAACGTGTTGTCTCCAGAAGGCTATGGGTTCAGATTGAGAGTATTGACTGAGCGTGACGAA +GTGTTGTATCTGAGAGCGATCGAAAGTGCTAGAAAAGAGAAAAGATCAGAATTGGAATCTATCTATTTGA +AGTTCAACCAAAAGTATCTTGGTTCCATTACTCATACCAGAACGATCTCTACTGTGTCACACAAATTCCC +ATTCTATTCACCAACAGTAAGGCTTTTCAAGAAGTGGCTTGACGATCAGCTGCTTCTACCACACTTGACT +GAAGAGTTGGTAGAGTTAATTGCATTGAAGCCTTTTGTTGACCCAGCACAGTTTGACTCACCTTCATCAG +TGGAGAATGGGTTCTTGAAGATCTTGAACTTTCTTGCCAGTTGGAACTGGAAAGAAGAGCCCCTAATCTT +GGATTTGAGTGCCAAGCAAGACGACATTGAAGACGCTAGCGAGATAGTTTCTAAGCTTTCTGATAAGATT +ACTGTTCAGTCATATCAGCAGATCAAGTCTAACTTCGACAACATCAGAAAGCAGGATCCGTTGGGCATGA +GAACGCAATTCTTTGTAGCTTCGAAAATTGACAAGTCTGGTATTTTGTGGTCTAACAGTGTTCCGCTACC +AATTGCAACCAGATTAACTGCTTTGTCCAAGGTTGCTATGCAGTTGGTTACTAACAATGGCGTGAAAAAT +GATACAATCAAGCTGCTCTTTACACCGTCTCTTGCTGACTATGATTTTGTCTTGAAGTTGAAGTTCACTC +CACTCACTCTGAGCTCAGGTGTCTTGCCAAAAGGAAAGAGGACCTTCAAGAACTTGGTTAACAACGCCTC +ATCATATCCTACTGAGCTTCACTCCAAGTATGATCCAGTGGCGCAACTGATTAAACAACTCTGCTTGAAG +TTCAAGGGTTCAGTAATTTTCTCTTACAAACAGAACAACATCGATGAAAGTGGAGAAAGTGTGATTTGTG +GTTTGTTTGTTCCACACACTTTACAAAAGACAAAGTTCAGAGTGCAGATTGGTTACAACTTTGAGCCCCT +CGAAAAGGACGTTGTTATCATCAATAAGGAGGCCATTTTCAATGAGATTTTGAACTTTGCTGGAGATTTA +GTTGTTGGGTTTGAGAAGAAATAGTTAATGAAAGTTATGTATTAATCTTGAACCACAGAGTATGATTTGT +TATATATAAGAAGCATACTTAGCTACAACTGTAGTCGGTGGATTTAGGACAAGAACACGTCCTAAACCCA +AAGATTTTTTTAACTCGGTATTCCGAGGGCATGCCATGGCGATCAGATCATCGAACTGTCATGAGGAGTT +TTTATTGTTTAACAATGCGTTCTGTAAGAATGGTGGAAGTTTCATAGACCACTTTCTTCTTTCATTTATA +TCTACTGATATCGACGGCAAATGATCAGGACAAGAGCTTTGGTGCTGAGAAGACAGTGGACTGCACGATT +GCCAGTCCCGCATCTATTCCATTCTGTGTGTCCAAGACCTAACAAAGAGCCAACAAAGGTCATTCTCAAG +TTGAAGAGCAATAGACAGATTTATCCAGATGCAAAGAAGGAATGGGAGCGTAAGCTCTACCTTGACTCTT +TACCCACACTGATGCGCAATGTTCGTGAAACAATGGACAGGTACAAGGGCTACATCGTCATTACGCAGGT +TGGCAGTTTCTATGAACTCTACTTTGAACATGCTACTGAAATTGCTCCAAGGCTGAACATCACACTCACT +AAGAAGACATTCAAGGAATTTACTGTACCAATGGCTGGATTTCCCCTGTCTCAACTGGATAGACACCTTA +AGGTGCTTGTAAAGGATTTGAACATGGGCGTCGCAGTTTCAGAACAGTTTCAGAGGGAAACCTCAATTGA +TAACGACCCAGTGAAGATTGCGAGACGTGTTACAAGAGTGATCTCTCCAGGTACGCTTGTTGATGAGGCT +TTTTTGAACGAGCAGGAAAACAACTATCTGTTGGTTATTGATATTCCCAAGAATTCCGTTAACAGGCCAG +CAGATCCAGACATAGCTGTTGGGCTAGCATGGATTGATATATCTGTTGGAACATTGTTTGTGCAAGAAGT +TACACTTGAAGGTCTAGCGTCAGCCATTTCGAGGATAAAGCCTTCTGAAATTCTGTTGAGTGAAGATTTA +GTTGAGTACAGGCTTGAAAGCGGAGATTGGTACCAGGAACTTATAGAATTGAATAAGTATTTTGTAAAGT +ACCAGAACTTACCAGCGCGACACAAGACGTTGGACCTATTCTATGGCATGTTTAACTTCACCCAATCTGT +CATCAAAAAGTCATTTGATATTCTTTCAGTAAAAGAGGCTTCCGCAATGAAAAATTTGTTGAATTACATC +GAGGAACACTTCCCTGAAACGAAAATAGGTCTTCAGTTACCAGAGAAACAGTATCCTGAGATGATTATGC +AAATTGATTCCAGAACATCAGATGCCTTAGAGTTACACAAGACATACAGGGACCAGTTGGCGAAGGGTTC +TCTTTTGTCTTGTGTCAGGAGGACATCCACACAAGGTGGTGCTCGACTCCTTTCCCAATGGCTAATGGCA +CCTTCGACAAATGTCAATGAAATCTTGTCTAGACAGAAAATGGTTACAGTATTTTTTGAAAAGCCAATTT +TTAGAAAGCAGATTGTAATGAGATTGAAGGAATGTCACGATATCAGCAGAATCGTTCAGAGATTTTCAAT +GGGAAGGGGTAGTATTCTTGACCTTGTTCAACTCGCTACAACAGTCCAGACCTTTGAATGTATCAAGGAA +ATTATCAATGAGAAACTTACCAATGATGACAATGCTATCAAGTCACTCCCACCAATACCAGAATCGGAAA +TGAAGTTAGCTAAAGAGATTCTCAAGGATATCGATGTGGAACATCTACTCACTGATCTGGAAAATCAACA +GAAAGTTGCTAAAGAGGAATTAGAATCTCTAGCTAGCGAAACCTACAGCTACTCAACTGCCAATAGAACA +ACTGCAAAAACTTCGCCACCCCTGTATGTTATCAGAAAGGAATCCTCCAAAACTTTGAAGTCACTCCATG +ATCAAGTCGATGCGTTACAGCAGCAACGTCTCGATCTCGAGATTATGTTGAGGGACAAATTGAAGACTGA +AGGTGTATTTAAAGAGGTGTCATTGAAGCTGAGTCCAAGTTTGGGATATCAAGTATACATCAAAGGAACA +AAGACAAGTAAATACGATGACCTTAGCAGATTGTTTCCCGAGGGTGTATTGAACCAAAAGAGTATGTATA +CAAGATGGTACAGTTACCCAAAGTTGACATCATTGGGGCATGCTATTGATGCGATGAGGGTTAGAATTGG +AAAGGAGGAGAATAATGTTGTGAAATCGCTGATAGCAAAAGTCATCAAGAGAAGCATCCATTTAAGAAAC +ATTGGGAAGTTTCTTGACTCACTTGATGTGACTAGTTCTTTTGCCATTTTGGCACATGAGAGGGGGCTTG +TGTGCCCCGAAGTTAATGGAACGACAGATCTTGAAATTGTTGATGGGCGACATCTTGCTGTTGAAGATTC +GTTGAATGCTGAGATGAAGCGTTTCACTTCAAACGATCTGACACTTCACTCGGACCAGTTTGCATGGATC +ATCACTGGTCCAAATATGGGTGGGAAATCGACATTTTTGAGACAGAATGCCATTATTGTTATTCTAGCAC +AGATTGGGTCGTATATCCCAGCCACTCGTGCCACGATTGGTGTTGTAGATAAGATGTTCAGTCGCGTGAA +TTCTACGGATGACTTATCTAACAATATGAGCACATTTATGGTTGAGATGGTAGAGACAAGTTACATATTG +AATGGTGCCACAGATAGATCGTTGGCCATTCTGGATGAAATTGGAAGAGGTACAAGTTCCCGAGAGGGAA +CAGCAGTGGCATTTGCTACACTTAACCACCTGGTTGATCAAAATAAGTCGAGAATCCTATTCTCTACTCA +TTACGGTAATGAGATTAGCCGTTTAATCAAGAAGTATCAAACTACCGGAATTGAGACCTTCGCATTTAAG +AAAATTACAGCTGAACGAGTGAGCGGACATCTTCATTTCAATAGAAAATTAGAAGATGGTATTTCTGAAC +AGTCATATGCTATTGAGGTGGCGGAGAAAGCTGGTTTCCCACCTGATGCTATTGCAGTTGCCAGAGAAGT +GATGAATTTGAAGATCGAGAATTCACATTGATAAAATTCTCGTCACCTCAAGATAATAAATCCACGCACA +ATTTGCTGCAGATTCACGTATCAAAACAGCTTTTTATTTTTGTAGCTGCATCGACGTCTAGGCCTTGTAA +CACAGCCACGCAAAGAATCCCACAAAAAATCAATACACACACCATACCACACTTGAGTATGGCATTCAAT +AATTAGATAATGAGTAATTTAGCCCTGGGTTAACGCACTTAAACACTAAAGTTCCACTCGTGAGCCTCTT +TCTCTCCCCACTGGGGTTGTCCCTATATTGATAGCTACTGAGCAGGATATTCGAGACCCCAGTGGGGGTA +GGGTGGGGCTACAGTATTGTGTACAAGAAAAGGCAGTGCTGTGAAATCAGCGCCATTCAAACAAGTTGAA +AAAATATTGTTTGCCCAGTCTTCTTTGAATTCGCACATAGCAATAGCAATGATTGTTTTTGAAAGAATGT +GGTTAAATTAGATCTCCACGTTGCTTCAAAAAGACACGATTATGCAGGTGTTCTATCCCTTTTAATAAAA +ATGACGTCATCGTCAGAAAGAAATGGAAAAGCGAACAAAAAAGAATAAAAAAGAAAAAATCATTCCGTCA +TATCCGTGTCTCGGATGGATTGTGGTAATTGCTCACTGAGTAGTCAGTACTACCTGCTAAAGATAGAATG +AAAATACTTTGAGAAGCTTTTAAAGTTGTTCCATTGCCAAAAAGTCCATAGCTTATTTCCCGATTAGGTA +TCAAATGCCGCGAGACGTGCATATCTGACGAATGCAGGTTCCAGGAACAGATAAATTGCTCCAATAAGCT +GTGAGTTGAGAAAGTCGTCATTGTCGCATTGGTCTGTTAGAATTGCAAAAATTCAGTTCTCTTATGGGTA +AAAAAGATACTGTGGTTGTTTTAATTAGGAGGTGAAGTTCCTGCACAGAGACATTGCTTTTCAAAATTTT +GCACAGGTGCAACTTTAATCAGTCACGTCCAGTGGGGCCTCAAGATTGCGCTGCCCTAATTGAGCAGAGA +AATTAAATTAAGCCCTTCGTACAGATTCCAAGATTTAATTAAACTTATGTTATGGCCACATTGATTGGCA +ATCTGAGAAGTTTTCCCTGTGGGTATAAATTGTAGATAAAATGTCTTCATATCTTCTCTTTTTTACATAC +CACCACATAGACACAAACACTAGCCGATAATGAAGTTCTCTACTGTTACTGCCATTGCTGCCATTGTTGC +CACTGCTGCTGCCCAAAATAACTCTAATGAAACCTCCTCTAATGCGGCCGTTGTTGGCTCTGCCCCAGCC +TATGGTGCTGCCGTCGGTGCCATTGCTGTTGGTGCCGCACTCTTGATTTAGACTACAGTCTGTTGTTGAA +AGTACATTGTTGATCGATAAAGCTTTTGTATTGTTATGGTTAATGTCTAATTAAGGCGAGGTTGATTGTA +GTTTGTTCAATTAAAATCTCTAGGGGTTTTTTGACGCTTTCCACCGCGAGAAATTTCATTCTTTTGTTGC +GATGGGGCTGTGCTGTGTCCAACAACAATTGCTGGCAAATTTTCTAGCATAAGTTGATGCGGATTTCATA +ACATAGCTGCGAGCTTATCTCCTTCTCCTCCGTAATTCTTCAGAAACGACTGTTCTATTGTGGCGAAGAG +CACAATCTCATTTACCACGGACAAAATCAATGCAGTCAGGATAGGACTGCGGAAGGTGGGAAGGTCTGTA +ATGTATGACTGTCAACCTCTGTCAACCTGTTCAATCTTTGGTCTGTAGTCTGTGAACACAGTAGTGATGT +TGATGAGCATATCTGCATCATTTTGGGAGCATGGGCTGCCTAAAAGATGTTCACGTATAACATAAAAAAA +ACTGGTTTGCTTGATCAGCATGGTCTCTCTGCCTCGATGAGTTTTCAGGATTGTCTTACAGTCTCAGACT +CCAGACTTCAGTATCCTTGTACAGAAATGAAAGACAATTAATTGCCTTTTCAGTTTTATATGTCTATTGC +ATAGGCTTGACCAATGTCTAGCGAGAAGGTGCTCTGTTGACAAAAAAATGGGCAACTGAAGACAAGTTAC +TGCCCTTGATAAGCATAATTGACGCTCTAATATCTCAATGTAGTAATAGAGTTACCTACACCCAGAGGAG +TGCCACTATGACTTTCTTGATGCCCATTTTCAAAGTTGACACAATTTTTTAGTAAGAAGAAGCTTGAAAA +AAAAAGTTTCACTGGCAGAAATCTAAAATCAATCACATTTGTAAGAACTCCACCACTGTCAGAGCTTCAA +ATCTTATCCGCCATGAGTGATCTTCTAGCGAGTGACTCGCGACCACCATTGTTCACTCTCAGAAAGCACA +CTCACCCAATCACGTCCCTCCAATTCATCAAAACACCATTAGTGACATTCAAACAGATTACAGCAGCTAA +GACTCAGGCAATCCAATTGAAACCATACCTAGTGTCTGTTGACGAATCAGGAGTACTGATTGTTTGGGAT +CTTTCAACGCATAGGCCGGTACTACAATTCCAAGCACATTCATCTCAAATCATGTCTGTGCGACAGATGG +GAGTTGTAGATGACATAGTTGATTGGAGTTACTTTGGCCTGGTGCTGACACATGGAAGAGATCACTGTAT +CAAGTTCTGGAGATTATTTGGACCTGATGGTGTTATGAAGGAAGAGATGGTGTATGAGCTTCCCGTCAAT +GCCTTGAATTTCAGTAATGTTGATATCTTTGACCAATTGTTAGTAACTCCTAATACAAGAGATTCTGAGG +CATTCGATGTTTATGATGTGTCATTTCTACGAAGTGATGGCGAGTTGGATGATCGAAGACTGAGGAGAGT +GCTTGAATCTGTGGATATCTTTAAACAGATTAAAGGTTCTGAATTTGATGAGATTAGAGCCAGCGGAGAC +GACGATGGAGATGTAAATAGGAGCGGGAAATTTGGAATCATTATGAAGTTGCTTTGGACCGTTGAGAACA +TATTGTTCATTGGATATGAGTCTGGACACGTTGCACAGGTTCAACTAAACTTAAGCTCCCAATCAATCAA +AGTTGTTTCTGTCGCTACACACCATTTTCCTGACCCTGTTCTATCGTTGACATTTGATGTAACAAACAAC +ACAGTGATATCAACATCGATGAAACCAGAAATTGCGCTGCACTCCATTGGATCTACACGCTCATCATTGA +TTACACTCGACAAGGGATCAGGATCAGGAAAAATTGCTAGTGTCGGATTCATTGACGACAGATATGTATT +TGCATCTTGGAATGGAGCTATAAAGTTCTATAAACTCAATAAGGAGTTATTGGAGTTGGAATATGAGTCA +AGTTTTAGGAAACCAAAGGGTATGATAACTGGTAATTTGAACATAGTTGGGAACTTGAATGAAAATGACG +ATGTTAAGACAAAGAATGCGAAGATCACGAAACCAAATGCACTGTCAATTACAAAGGGATCTGAGTTGAG +AAGGGACATCGTATTAAGGAATAGAAGGGATGCTAAAGTGATTGAGAATGATTTCATGGCTGTTGGGTAC +AGTGATGGGACAGTAATGATGTATACATCTTCTTGATTGGCATTAATTTGACAAGCTTCTATGAACTGCC +CAAACTCTTCCAGTTGGCAAGGAGTACAGAGTTAAGGGCGAGAAACTGATAGTATTCATCTCAAAAAAGA +GACATACAATTGATAGAATAAAGACGTTTTGCATACATAAGTTCAAAATATTTGCTGAGTACGTGTTTTA +AGTGATGAAAGTGGAGCCTTGAGGATAATATTTGACAATGTCTCATTCTCTGCCACAGATGGAGCAGCAT +AAGCTAATGAATTATCGGAAGAGGTTGTATTTCTATTCTTCAAATTTATTTTCAGCAATGAGCGTGCTGG +CTATTCTGGATACTCATTTTTCAATAGACGGTAGACGATTTTGGCACATTTGTAGGCCCAGGGATGCTTT +TTCAAAGTACAGGAGTAGTTGTACCAACATAAGAATCAAGCACGACTGCTCAAAAGGTGAAAACTATGTC +TGAAGCAAGCTAGTCGGCTTCCATGAGCTTTATCGAAAAAAGTTCAAGAATATCTTCATGCAGAGAAGGT +TTTATTGACAATGAAGATCTCATGATCAGCCGTTTGCCTTAAAAAACCGTCATTATGTTCAAAACTCCCG +TCGAAAAAGTTACATGAGGATGACCTACAAATATACCTATGGCGTAACTTTCTTTAATATTTTCTTTTTT +CCTTTAGCTATAGACATGGGGTGCTACTATTAGAGGGATTCACAAACGATCATCCTCAGCCTCTGTCAAG +TCACCATCGCTCAGCAAATCGGTGTCATCATGCTGGGTAGTATGCAAAGAGGCCTCGTCTAAGATGTCAT +CAGAGTATGCAGTGGCCGTAGCAGAGCTGTAGTCGAGAGAGACACTCTTCTTAAAGAGCTTCTCCCTGAT +CAACTCAATACCAGGTTCAGTCACTGTGCTGATAAAACCAACTGTTACGACAACGACATAGCCCACAATT +GATGAGATTCTGTTGAATGTGGTGTCAGTGTCAAATTCAACTGGTTCATTGTCATCACCAAGCTTGATCT +GGCCATAACGACCAACATAATGGTTGTAGATAACATAGATTAAAACACCAGCACCAATAATTGGAATCAA +AATGACTAGAGCCAATGAGAAACCTCTCAATCCGTGCTTTCTGTTGAACTCTTCCTCACGACCAGGACAT +GGCCTAGCCTCAACCTTGTCAAACTCCTGACCACCGGAACATGTTGACAATGGAATCTTTCTGTAACCAG +TTGGCAACCAGTACTCATCACCGCCAAATTCTTTACAGACAGCAGAGGTGTCTGGCGGAGTGTAGCCTGG +AACTAACTTACAATTACCCGCAGAGTCCCTGTAATAGTTAAAATCACATTCGAAATCTTGACGTGTGCAT +GGACAGTTTCTCATCTCTTCATATGGCTTGAGCAACTTCTCACCGATGTAACAGTCGGCACCTGGCACCT +TTCTGTAATATTTCGCCTCATGACCAAACAAGCAGTTGTCAGCCTGATGTGGGTGACGCGGAGTCCATAG +ATCAAAGTCATCAGTATCTGGGTGTTGCAAGTCTAGGGAACACTTTCTCTTTAGTAGTTGGGAGAAGTCA +ATCTGATACACGGTAGCTCTGTCACCTGAAGAGGCTGGTAGACGAGTGAATAGAAGGAACTTTCTCGAAT +CATCCGATGGAACAGTGGAGAGGTCGTTGACCACAACCAACTCATCAGTGAACTTGTATTCATTCCAAGT +GTCACCTTCATCCAAAGAGTAGTACAAGACATTAGTGGAGTCCTGTCCGTTAACTAGGACCAAGATGGAT +CCTTGATCACCGTACTCCCATTGGTAAACACCTTTCTTCACTTCTTTCCAGGTAACACCACCATCACGGG +TCAAGTAAGTATTACCATCGAGGTATTGGCCCAGCTCTGAACCAACATTTCCAACACCAACCATCATACC +AATCGCAGAACCAGAGGAGAAAGTGTCTCTGTAATCAGCACGTTCAGTATAACCATGCAAGTGCAAGGCG +CATTCCTTTAGTGGCTTGCCGAAACATTTATTCTTCTTTCCTTCAGAGTCAACAGCTGGTGGTTGCAAGA +AGTCCCAGTCAGCACCGTCATTATGTGTGGTTTTAGATTGCAATTTCTTTTTAGAACCTGTCTGTGCTTC +GTTTGGATTCGACACAGTGTTGATTATGGCGACACCCTCCAAGTTCTCCATCTGTTCAAAGTCAACAAAT +CCAGAGGAGTCTCTGTTGACATATTGCTGGGACATGACATAAGAAGTACCGTTAGAGTTGGACTTGAGGA +TAGCACCAAACTCAGTGCCTGTTCTGGCATTGGTGGTGACGTGTAGGAAGATTGCATGCGATTGGGCAGA +AAGGACTGTGTACGCTTGTCTCTTGTCGACTCTGAAATTGGATGGGAATTCAGCATCAGCCCAAGTCTTA +CCATCAACTGAGACGTGGGCTCTTAGCGAGCCATCCTCAGAAGAGGTGGCAGCAACAAGGAAGTCGTCTG +CTTGAACCATACCAATAACATTGGACAGTGGAGATGATGAGGTTTTAAAGTAATCCTCAGAGAAGATCAA +CTCCTCGGAATGGACAGTGTGCTCGTTCAACTCTCTGGTACAGACAATCAAACTTTCATGAGTTGGTTCG +GCAAGATGGCCAATGTAGTCACATGAGTTAACGTGTTCTTGCAACAATTGGAAGCGCTTACCGTATGATT +GTGTTCTATACGTGACAGTACGACAGGCACCTGTGTGGGATGTATCACAGTCCTTCTGACCAACGTAGAT +GAACCAGTTGGAGTGACGAGCGTGAAAACGAAGAGGATTGACACCAGGAATGAAGTTGGCTGGGGTTCTG +AAGGACTTCCAATTATCAGCTCTATCAGTCGAGTAGATGATCTTCTGGTTGGTGGATAGCAAATAGACGT +GGTTTGTATTGTATGGGTTTACAAAAATGTCGAGGATCTCCTCCTCTGGAGCAATCTGTTCCCAAGTGGA +ACCTTGGTCATGTGTCACATAGCCTCTGTTTAGGGAGGTTAACAGCACGATTGTCTCATCTTTCAAGTTA +GATCCAGGGACACGGTCCAAGTACACGTACTTCACAATCTTACCGCTGAATCCAAAGGCAGATGTTCTTA +CTTTACCGTCATTTTTACCATCTTTAGAGTCCTTCTTATCACCTTCACGTTCATCTGGGTTTCCGTCGTC +TTTCTCGGTCTCATCGGGTTTGTCATCACGGTCATAGTCAATGATCTTACCAGCACGACGATCAGATGGG +TCCTCTGTTACGATTAAAGCCTCCGTGATGGAGGTGCTTGTTGGCTCTGGTGCCTCAGTGGTGGACGCAG +CAGCATCTTCCTGGATCGTAACAGCAGCCTCATCTTCGCTGTTGATAACCGCAGCAGCAACATCAGCAGC +CCTAGCCACAGCTGCGGTGGCGACAAGCAACTTCAGCAATCTCATTGTAATGGATAGTGAGAACCGATGG +AGTCGTATGAGATTGAGTCTGAATTGAAAAGATTTCAGTTTCCACTGCTTTTTTCTTCGAATTCAGGGTG +CCAAAAAGATACGAAAGAAGTTAAATCCGAACGCCGAAGTCTCCGAATGTGGCGCGAACCGTTTATTGAG +GGTGCTGTTATCGGGGATAGTTTTCCTACCGGAAATATCTGGCTTGGACCACATCCAGAAGAGGTGAACG +TATGTGTCAGTGTGTATTTAGGGTATTTACCGGGTGTTCGATTGGCCACGCCAAGTTTTCTTTTCTTTTG +CAAAATTTCAGTTTAGTTTGATCGTTTCAGTATTAAGTCATTAAATACAGTGGCCTAATAATCAACTGGT +TGAGAACCAGGTGCCGAGCCTCTGCCTTCACTTCTTCCCCCATCGCGCAAATATCTATGTTTGCTACTTA +TAGAACCTCTGTCTACCTCCCTTCTGTCATTCCCTTGTCTTCCTTCTGAGTGTCTATTTTCAAAATCTCT +ATGCCTTCTATCAGAATCATACTTCTTCTTATCAGAGTGATTATGATGAGATCTATGAGATCTATGATGC +CCATGATCTCTACTTCTTTTAGGTGATCTCTCTCTTTGTCTCTCATCTTCCCTGAGTTTCCTCTCCATAG +CCACTTCTCGCAGCTTTTTCATTCTCTGTTCTTGAATTCTTGCCATCGGATCCTCTCGAGATCGATTAAT +CTCCTCCATTTGGCTGAGCATTCCCTTATTTCCAGCTGCCACACGATCAAATCCTGTGGCCTTGGATTCG +TTCTTCTTGTTCGGATTGACAATGAGATCATCAACCCTCTTTTTACCTAAAAGATAATCTTCATTTGCAT +TAGAGGCCAATTTATTGCCATCTCCAACACCTCCAGCATCATACATCCAATCAACTCTATCGACAACTCT +CTTTCCGGTTTGCTTATATTGTAATTCACGAAGCTCAGCGAGTTCCTGTTCTTTTGCTATCTCAGCTTTG +CGTTCCTGGATTCTCTTTCGTTCCGCTAGTGCCTCCTGCTCTTTCTCCCAGACTTTTTGCTGGTTCTTCA +TGAGAGCAGGATTCCATGATTTCTTAAGGTTTAAGTCACCAGGAGCCATTGTATTTGGTGTTCGTTGTGC +TGTATTGTGATTTGGTTTGGTGTCAATAGTGAGAGGTATAATTTTTTTTCTTTTGCCCTTCTAAGCGACT +ACCTCCTCGCCCTTGCTGATGTAATCAGTATTGTAATATACTTAGACAGACTGATTCATTCTAACTGGTC +TTATACAAAAAGAGGTGGTTCTATATACGTAGTTATAGACACGCGCAGCACTGTGGATCTTATTATTCCT +TCTGCTCCGTAGCATGTATTCTCTTTCAGCTACTCTTTTTTTTTGCAAATGCAGGTCAGGTCAGGTCAAG +GAATACACGCAAAATTTTTCACAGCATCGCTTATCTCTGCCTGAAAATGGAAACTTGCTCATGATGAAGG +AGAGAGAAGCCCGACGAGAGATAGGCCATGTGAAGATGCGACAGAGTAGTATCTACGAAGATACCGATAG +CAACCAAAGTATCGATTGATGCTAATGTTGCTCATATTGAAACTAACCTATCTGGTCTTATCATCCCCAC +AATTCACTGTTCAGAGCTCGCAACCACGACGTCACGGACCAAATTCCAGAGACAGACAAGATGATGCGAT +GCGAATGGGAATGGATCCCCGGGTATCACCGGTCTGCTGCATCAGTCTTCGTTGGGAACGTCATCGGGAT +CTGTTGTCACAGATCTTTCCCCTTTCCAGAAGCGCAAGGCGAATGCGGTGTTGTGATTGTTATCTATGTG +GATGATAAGCCACAACGGCGGTGAAACAAAAACATTGCCAAACGCAGATAACCAGCTCCATAGTTAAGAA +AAGGTTTCACATACAAATTTTAAATGTGCTGATTAGCAGGATATCACCAGCCAATGAGAAAGGAGAGGAG +ATCTGTATTTATCAGATCATGTTGATGTTTGAGATAAGGCCGTAATGGGTCGTCCCGACTCGACTAGAGC +TGCTGCTGTCAACACCTGTCAACTTCGTGTAAAAATTTAAGTTTTTGCAGTTCTACTTGAGTTTGTATAT +ATCCTGCATCCATCTTCCAGTTCTTACTCTTGAGCTTCACGTGTCATGATCGAGGAGGCTTAGCTTAGTT +GTACTGCATCCTAACACATGTTTCTCTAATCTACGTGCATCTGAATAGCAATCATTTTTTCTCCTTCTGT +GATCATCCCCCCTCCAATTCGAGTGGGCGATATCAGAATGTTTGTTGGGTTTTCCTGGTTTTGAGTTGGT +ATCACCCGAAGATAAGCTAGATACAGCCCAGTAATCCCACAATGATTGTTTCGCTATCTCCATAAATGCT +ATATGTAGTAGTAGATTGTTTCTACCATTATCTCTATACTTACTATAGTCTATATGCCACATGTTTTGAC +TTGTGCCTTGCGCACATGTCTCACTGCATGGGGCAGATCGCACATGTGCCAGTGGGCTTCTCTCGAAAGC +AACTTTATTTTAGTGGCACATGTCAAAATAAAAAAAAAACTTCCCTCATTTCGAGCAGCCCACATGCGTT +GGTTGGTGAATGCACACAATAGTTGGGGAACTCGTGGGTGGTAGCCCACTACGCATGCAACAGATTCTGA +GGTCATCTGCAGTTTACAAGGGCCACATGGACTAACCAGCCATAGAAGAACTAGTGAAATTTAGTACACG +TTCCTTACACGTTCCCTACACGTTGCCTGTGGGTGCTGATTTCAATCCCTACCAGAAAGAAAGAAAAAAT +AACAACAAAAGAGGGTGTTTTGCGTAATGGTCATTCGAATCATCTTTCTCGAAGGATTACTGCACACAAC +AACAGGTCAATCAAATATCCACCCTCCTTATCGCCAGGAACATGTGTGTACAGTATAAAGCGTCATAAGT +CTAAGCATATGCGAGCCGGAGCATCTAAGTCAGTATTATAAAGTCCTGCTTTCAACAATATTCTCTAACA +ACGATTTCAATGATCCAAGGTGTAATAGTCAAGATCATAGATTCGATTCAAAAATAGCACTAATCTGTAG +CGTCTGCGGTGTCTTCTATTCTTGGATTATATTCAATCAGAAGCGAAAACAGTCAATTTACACAGTTTCC +TTTGAATTTTAAACATTCTTTCTAATGTTCAGTTGATACCTCGACAAGTACATATTTCTACAAAGCTTAA +CAAAGAAAAGGAGTGAACATCAAGAAGTGGGCAGAGGGACATTAGGCAAATAAAACAACTGTGTAAATGA +ATGGCAAAATGAAAACCCCACTCTATTCGATGCTACGGTTATAAATAAATATGTTCTGAGTAATGCACAG +GGTTTGGAGATCACAGATCAACAAACACCTCGACTTTTGTTCATTAATCACCACTCATAACCATACCACA +GCCAAAAAACAAGACAAAAGACAAGAACAGCAACAACTAACAATGAAAATCTAACCACGTGAATTGTACC +GAGCTGTAGATGAGATCATTAGATAGATTCAGCTATATCCTAATCACACGCAGCAAGGGTTCTTAACTCC +AGTGGACTGAGGGGAATTCAAAGAGGATGTTCTGGGCCATAACGTACATGAACAAGGCTATCTATCGGTA +ATGACTTTTACACAAGTGCGTGATCCTATTTTGTGACTACCGAAATGGTAACTCAAGTTTGTTGTTTGTA +AGAAAGATCGAAAACTAACATAGGGAGGTGCGATACTTAATACTAGAAGACAGGCGAGCCCACACATACA +CGCACATACTCTTTCGTACTCTCTTAACAATACAAATCCGTGGTAGACGGATCATCACACCCCACCATAG +AACTCTCCTAAACAACAGGTGCTCTCTCTCGATACTCAGAACATAGTGGAAATCGAAAAAAAATGTGTCA +GTAGTCTGTGTGTATGTGTGTATTTGTGTGTGCCCACCAGAGAAACGGAGTAAGAAGTTAATCCTTCATC +GTGCTTAACGATGCCATATTGGAGAGCTGATTGATTAGCTGAATGATCACGTTATAGCAAACAAAAGTCA +TTAAGGGGGCAAAACCCCATAATGGTATATACAGCCCTATTATGTTAAAAAATAAGCTGAGAGTTGTGAA +AGTTGATCTCAATTGCTCTCAACCTTTGAGCACTTTTGAGTGGGGACAAGCTGATGGAAATGGAATTGGA +CGAGAGCCTAGGTGTGTATGTGTGTCAGGAACTGCATAACTATGTAGTCAGCGCAGGTTCTCGAGTAGTG +CGGACAAAGCTCCAGCTAATATGTATTATTTTGCACTCTCCTTATTGGCCAGTTGATGCCTTCGTTTATG +AAAACTTTTCTTCAGTTACCAACAAAACTGTAGAAGCCGTTGGAATCCCGCACGGAGTTGCAGATGAGTC +GAGGTGGGGGCTAGGTGGAGCTTCTGCCTAAAGTTGGTTAACTCCGGCAGGGATGCTCCTAAAGCGGAAT +GCCGCAACATGGATCCAACTTGCATAGATTTTACAGTTATCTGCAAAAAAAAAAGAAATTCTAGAATTTT +TTTTTTTTGAAGGAAGCTCATCAGCAGGTTGATATAAAACAAGAAATCCCCATCAAAACAATTGGTGATC +TTTGATATAAATAGTGTATATCACATTAGGTTCTTGGGATTTTTAACCTTCCACACCTTTATTAATTTTT +AACTATTCGAATTGATTTGCTTCGCACTTCCTTTTAATTCAATTATATCAGTGTTTAAAACTAATTCTTG +ATACTTTTTGAACAGAAGGTAGCAATCTTTTCAGTACTTTCCTCCCCCCCTTTTTAAGAAATTTTTTAAA +CCCTTATACCCACCCACCCTTTGTTATGGCTCCATCTACTCGTAAGTCCACTCGTGTTCGTTTTTTAACT +TCCAAAGCTAAACATTCAGAAGAAACCTCCAAGAATTTGGTACAACCATCGATGAGCAACAACATCAACA +ACACACACACCACAAGTGTTTCCAGGAAGCCAGTGTTGAGATCTATTTCCACCGTTGATGACAATGAGAA +CACATACAATAACCTCAACTTCAAGAGAAGAAAACTTCAGAACACTTCTGTCGTGCAGGCACCAGAAGCA +CCTGCCGATGATGAATCTGAAGATGAATACACCCAACCAAATTTTAGAGTCCCAGATGACTTTGTCTCCA +TGAGCGAGGAGGAATTCCTTGGTCAATCCGAGGACGATAACTTGTTCAATTCTGACGATGACAATGAGTA +CTACAGTGCTATTTCTGACTATGAATCTGATAACTTGTTTAGCAAACCAACAGCAAACAGTCAACAGCAC +AAGAGATTCATCAGCCACTCAAAGGCTTTTCTATTCAGAAAGAACCAAGACGCTGAGATTTCAAGATTGG +CTCATGTGAAGTTCATGCAAATGGAGAATGACAATTTGTTTGAGTCTGATGAATCTGAATCTGAGACTGG +TTCTCCAATCAACTCTCATTTCCACTTTGCTGACACTTTTGGGGAACTCAAGTCATTGGAGGAGAATTTC +AAGAAGATTATTGACGAAAACCACAAGTCCTCTAGTCAATCCACTGTATCTTCTGAACATGAGTCGTTAG +CTGCTCCAACCCAAGCGGAATTGAAGAATGACGAGAACATCACCATTGATGACTTTGTTGATTACACTTC +TGACGTTGTGTCTTCTGATGAAGACGCTGAAACCACCAACGTTCAAGAAGAACAACATGCGCCATTCAAG +TCTTTGTCTAAGCACTTGCACTACAGACATGATGAGAAGCACAATTTGGCTTTTCCTGATGTCTCCACTT +TTCCTATCAGATATGACCCATCTGCCAACAACAAGAAGACCACCAAGAAGTCTGTCAGAGCCCTGAATAG +AAGAGCTATCTTGACTGGTATGGCCAGTGAGATGGTTGGTACTGGTATGGCTATTGGTGAGTTCAATATC +TTTTAGAGTGCTTATATTGCTTTGCTTTGCCTTTTTTCCGTCTTTTTAGTTCAATGAAGACCCATCTTCA +TTCACTTTCAATACTGTTGTTTGATCCACAACAACTGGGTCCTTTTTTTCTTTACTTCATTATGGTTTGA +TTTTGATATTTGTTTTGATCGTTTTTTGTTTTAAGTTGCATTGATATCCTGAACTGTTATAGCCGGTTGC +ATTTGATTAAGGGTTTATAGAGATTTGGTTTTTTTTTACTATACTGAGCCAGGATACATTTAGTTTAAAT +TCATGGTGTGCTCCATTTATTTTGAAGGAGTATACATTACAATACATTTAGATATGGGAATTAAAAATTT +TGTAACTGCATCTAGGATCCATATATCTTTACTGTTTTGAGCCGCGGTTTGATGTCATCCGTCAAGCGGG +TTACCCAGGACAGAATTCATCAAACAGGACTTCTAACCGCATACTAAACTGGATAATTTCCGCTTTTGGT +CATTGTTTTTTTGTATATCACATGTGGAATTTTCCGCCCATTCGGCAGGAACAAATGACCACAATGAGCC +GAATTCTATTATTTACAAGCAGTTATCCTAGTTTCTGAATTGCAATTAGCATACATTCGTTTGTGCAGAA +TAAATATCCTACTTATTCTTCGTTCGTTCAGCTCAATAGTTCTACGACGAAAACTCACAAGCTTACTTAT +TGCAGCCTGCAGCACGCCCCACCTGTCAACAATTTTTTTCTCTGGGGTTATACAGCACCCTTGAAAACTT +TTCACTAGTACATATTACACACTCTTTTTCCTGCTAAGCGACCAAATGTTGAAGTCAACAGGACAGTTAC +TGAAGGATGTTTTCATACCTCTTCCAGCAAGTAAACGTGCACACACGCAATTGCACACAACCCATGGTGA +ACATCACCATCATGTTAGTGATGACTACAAGTTCCCTATAAACGACACACAATCGATCAGTGATGCCTCT +ACTTTGGTTCCACAGACCCACAGAAAACTAAAGAACAGACATGTGCAACTTATTGGTATCTCTGGTGTCA +TTGGTACTGCTCTTTTTGTTGCAATTGGTGGTGCACTACAAAAGGCTGGTCCTGGGTTCTTATTAATTGG +ATTCACCGTTTGGTGTATTCCAATTTTATGTATCACTGTCTCTACAGCAGAAATGGTGTGCTATCTACCG +ATAAACTCCCCCTTTATAAGAATGGCTGGCCGTTGCTGTGACGATGCTCTTGAATTTATGGCTGGCTGGA +ATTTCTGGTTCCTGGAATGTGTCTTGATTCCATTTGAAGTTGTCGCCGTGAACACAATTATTCATTACTG +GAGGGACGATTATTCCCCTGTGATCACCCTGATAGTGCAGATTGTTATTTATTTCTTGATTTCAATTTTT +GCTGTTAGAATTTATGGCGAGACTGAGTTTTGGTTGGCTATTGGTAAAGTTTTGCTGGCTGTTGGTTTAA +TTTTGTTTACTTTTATCACCATGGTTGGTGGTAACCCACAGCATCATGCATTTGGTTTCCAATTCTGGAA +GAATCCGGGTGCATTTAAGCCATACTGGTCGACTGGTTCATTAGGATATTTCCAAGCCTTCATTACATGC +CTCACACAGGCATCTTTCACCATAGCAGGACCAGACTATCTCTCCATGGTTGCTGGTGAAACTGTACTTC +CAAAGTCATCGACTCTTCCAAGAGCATTCAAGCAGATATTCTATAGATTGACCTTCTTGTTTATCGGAGG +TGCTCTATGTGTTGGTATTTTAGTTGCCAGTGATGACGCTGATTTAACTGCAGCCATTAATCAGTCAAGA +CCAGGTGCTGCTTCCAGTCCTTACGTCATTGCAATGAACAATATGGGAATTAAAGTATTGCCAGATATTG +TTAATGGTGCGTTGGTTGTTGCAGCTTTTAGCGCAGGGAATGCCTATACTTATTGTTCATCGAGAACACT +GTATGGTATGGCTCTCGAAGGAAAAGCTCCATTTATCTTCACATATTGTAACAAGCAGGGTGTCCCAATC +CTAGCTGTCCTTGTTTCATTGGCATGGGCACTATTGTCATTCTTGCAATTGAATAACAACTCCGCTGTTG +TCTTAAATTGGTTAATAAATCTGATCACTGCGTCGCAACTGATCAATTTTTGTTTGATGATGGTCACTTA +CTACTTTTTCTACAAAGCGGTATTGGCGCAAGGTATTAACAGAGATGAATTCACCTTCAAGAGTTGGTGG +CAACCATATACTAGTATTTTTGGCTTCACTTGTCCTTTCATTATGATGTGGGTCAACGGTTACACTGTCT +TCATCAAAGGACACTGGGAAGTTTCAGCCTTCCTGTTCAACTATCTGGCCATCTTTGTCTGTATTGGTCT +ATATAGTTTCTGGAAGGTCTTGAAGAGATGTAAGTGGAAAAACCCTCTGGAAGTAGATCTGGTGTCTGGG +TTGCAAGAGATTGAAGAACATGAGGCTCAATACTACCAAGAACTGGAGGAAATGAAGAGGCTCAAAGGTG +AGAAGAAAGGAATTGCCAAATTCTTTGACAGTGTCCTATCCCTACTTGTCGGAAAGGATTGATTATTTAT +TATAGAGCTACCTAGAATATATTTTAATTGTTGATATAGTTCTTTGGCTGTATAGGGTTTAATGGTGAAA +TCTCGATCGTGTCTCTATCAAAAAGTGCACAGCAAAGTGCGGAGATGAAGATCCCTGTTCGACGGAACAA +TAATTGTATACTATTTGACACAGCCCGATCACGCTATTTATAACTTGGGTACAGCCATTCTATCAAGTAT +TGTTTTGAAAAGTGGTCAATAATCAGATGTCTTACGATTAGTTATGTTAGTAATTTGAACTAGATTGGGG +GATAGTAAGCTAAAAAATAATGCAACTGACACCATCTACAAGGGGGTCATCTAGAACGGGGATTTCATTT +CTAGAATTAACTTCATGAGCCACAACCAAGCCTACTTACTTGCCCATTCTGGGTTAAGGTTTTAGTATAA +TCCTGGTATGAATTGCTATGAACTACACTCAGAACAGATTCCAGAGCTGTGCAATTAAGATATCTTGAGG +GAGAGGTGTCACATTTCCAAAGATATAAAAGGCCCAAGAGGTCTCTGCATAACCTTTAATATATTTCTTT +CGAACAGTACAAGAGCGTATTCATTCACTAATAATGCAACTCACTTCTTTAATCTCCTTGTTGTCCCTAG +CTGTCCTTGGCGTTTCCCAAGCTACGGACGCCCAAAGTGAAGGTGCAGTTCCAGCTACTACTACTGCGGC +TGATGCTGCCACTACTACCGATGCTGCTGCTGCTACTGATGCTGCGGGAGAAGACGATGCTGCCGACGAA +GACGATGGTACGGGAGAGGACGATGAGGATTATACTGATGATTACGATGAGGACTATACTGATGATTACG +ATGAGGATTATACCGATGATGCAGAGGATACAGCCACTGAAGCTTCTGAATCGTCGGCTGCAGAAACCCC +TGTGGTTTCACAAATTTCTGATGGCCAGGTTCAGGCCACTGCTACCCCAACTGTTGCTGTTCAATCTGAT +AACGGTGTTGGCAAATTCGCTCCAAGTGCTGCAGTTGCCATTGGTATCGCTGCCTTCTTGATATAATCTT +ATTGATCATGAATGATGCTATTCAAATCGTTTTATTTATCTCTCTTTTATTCTGCATTATGTAGTGACAG +GTATCGTAAGGATCATCACGTCATGACACATGAACTTCCAACGCACTACAAATGGTTCAAAAAAAACACA +CAGGATCTATGTATATTATGGAGGACTAATCCTTCTTTGAGTCCTTTGGCTCTCCTGCTTTCTCGCTGCT +GGCTTCTGTAGACTCTTTAGATTCATGCACTTCATCATCGTCCAAGTTTTTCTTATCTTGAACTTGAATG +ATAATACCATCATCAGTATCTTGCTTGGTTTCAGACTCAATCAGCTCTTGCTGTTCCTTCAGCAGTTCTA +ATCTTTGTTTGTTACTAACCTCTTCGACAACATCAGCCTCAGCAACATGGTACACTTCTTCAGGCATCGA +ACTTAGCACCGCTTGAAGTGCATCATAAATGGTTTCATCAGAGTCCAAATCACCGTAAGTGTATGCGTTG +GACAACACAAGCAGGGTGGATGGGATTCTCTCATTTAATCTCAAGTTTAACCAAGCAGTAAGATCGCGTT +TCAATTCGCTCACTGGGACACCACTGACCCTGATACCTCTAGAGCTGCATGCAGCTTGCAGCTCAACTGT +GCTCAAGCTATCAATACCCTCATAGTAAATGGCTTTGTCATCTCTCTTGATATTCAGCAGTTTGTTTCTG +ATTCTGAATGTAAGCATTTGATTGGTGCCGATTGGTTGAAGATTAATGTATTTTGCCACAGCAACCAGCT +GAGATCTTTGTAGGTTATCCAAGACAGTGTCATCTTTGAACAGTTTGGCTACTCTAAGTAGCTGTTCTCT +AGATGGCTGCTCTGAAGACGATCTAATCTTGACCAAGAAGTCTTTGAAATCCGCTTTTTGTTCCTCAGTA +AGAGCACTTGGAAACTTCATCTGGATAGAATCCTTCAGCAGTTGAGACACACTGGTTCTGGTCTTTAGGA +GCTTTGAACGCTTTTTTTCCTTGTCTGCCGAAGATTCATATGTTGATGGCAACAGGTTTGGGAACATCTT +CAAGGCGACTGGCAACAGAAGTTCAGCGAATGGGACAATCACAAACATCGAAAATGGAACAAGTCTAACG +ATATCTTGTGTAGTTCTTTGTAACTGTTTATTCTCCCTTCTGGTCAACTCGTAACCAGCAGCAGACTTCA +ACGCCAACTTGAAAGAGACTTTGATCTCAAATCCCAAAAGTTTAGTTCCTTCCCAGTAATGGTTCACTTC +ATGTTTCACCTTTTCCCAGATGGTCTTCTTAGCAGGCTCTGTGACCGTAGTAGTACTTT + diff --git a/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.lift.gff b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.lift.gff new file mode 100644 index 00000000..8335d0a0 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.lift.gff @@ -0,0 +1,22 @@ +##gff-version 3 +##PAF BC_dic1 316 0 316 - JAMAIE010000005.1:589225-655853 66629 16016 16964 948 948 0 AS:i:1604 ms:i:1604 np:i:316 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:316M cs:Z::316 +JAMAIE010000005.1:589225-655853 miniprot mRNA 16014 16964 1604 - . ID=L000001;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_dic1 1 316 +JAMAIE010000005.1:589225-655853 miniprot CDS 16014 16964 1604 - 0 Parent=L000001;Rank=1;Identity=1.0000;Target=BC_dic1 1 316 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 16014 16016 0 - 0 Parent=L000001;Rank=1 +##PAF BC_MATa2 269 0 269 - JAMAIE010000005.1:589225-655853 66629 24368 25231 807 807 0 AS:i:1366 ms:i:1395 np:i:269 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:96M56N173M cs:Z::96~gt56ag:173 +JAMAIE010000005.1:589225-655853 miniprot mRNA 24366 25231 1395 - . ID=L000002;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATa2 1 269 +JAMAIE010000005.1:589225-655853 miniprot CDS 24944 25231 477 - 0 Parent=L000002;Rank=1;Identity=1.0000;Target=BC_MATa2 1 96 +JAMAIE010000005.1:589225-655853 miniprot CDS 24366 24887 918 - 0 Parent=L000002;Rank=1;Identity=1.0000;Target=BC_MATa2 97 269 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 24366 24368 0 - 0 Parent=L000002;Rank=1 +##PAF BC_MATa1 162 0 162 + JAMAIE010000005.1:589225-655853 66629 25855 26341 486 486 0 AS:i:812 ms:i:812 np:i:162 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:162M cs:Z::162 +JAMAIE010000005.1:589225-655853 miniprot mRNA 25856 26344 812 + . ID=L000003;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATa1 1 162 +JAMAIE010000005.1:589225-655853 miniprot CDS 25856 26344 812 + 0 Parent=L000003;Rank=1;Identity=1.0000;Target=BC_MATa1 1 162 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 26342 26344 0 + 0 Parent=L000003;Rank=1 +##PAF BC_MATalpha1 233 0 233 + JAMAIE010000005.1:589225-655853 66629 29177 29876 699 699 0 AS:i:1232 ms:i:1232 np:i:233 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:233M cs:Z::233 +JAMAIE010000005.1:589225-655853 miniprot mRNA 29178 29879 1232 + . ID=L000004;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_MATalpha1 1 233 +JAMAIE010000005.1:589225-655853 miniprot CDS 29178 29879 1232 + 0 Parent=L000004;Rank=1;Identity=1.0000;Target=BC_MATalpha1 1 233 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 29877 29879 0 + 0 Parent=L000004;Rank=1 +##PAF BC_sla2 1046 0 1046 - JAMAIE010000005.1:589225-655853 66629 38509 41647 3138 3138 0 AS:i:5179 ms:i:5179 np:i:1046 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:1046M cs:Z::1046 +JAMAIE010000005.1:589225-655853 miniprot mRNA 38507 41647 5179 - . ID=L000005;Rank=1;Identity=1.0000;Positive=1.0000;Target=BC_sla2 1 1046 +JAMAIE010000005.1:589225-655853 miniprot CDS 38507 41647 5179 - 0 Parent=L000005;Rank=1;Identity=1.0000;Target=BC_sla2 1 1046 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 38507 38509 0 - 0 Parent=L000005;Rank=1 diff --git a/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.miniprot.gff b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.miniprot.gff new file mode 100644 index 00000000..ff4fc5a7 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.miniprot.gff @@ -0,0 +1,84 @@ +##gff-version 3 +##PAF MH260578_SLA2 1072 29 1072 - JAMAIE010000005.1:589225-655853 66629 38512 41626 1719 3165 0 AS:i:2775 ms:i:2775 np:i:763 fs:i:0 st:i:0 da:i:21 do:i:3 cg:Z:94M2D73M1D99M1D18M7I14M2I256M5I54M4D136M1I29M1I11M1I183M4D59M cs:Z:*tctT:1*ctcI*cagS:2*cttI*cgtK:2*acgC*tctT*gctP*gatE*gagD:2*ccaS:18*ctcK*tcgD:2*aacE*gctT*ttaM:1*attV*ttgV:1*attL*cagK:1*gatT*gaaD*gttT:7*accL:9*caaP:3*gcaV*gaaG:1*tacF*aggK:1*ttgM*aacE:2*gatS*ggtN:1*gggD*agaT*tctL*ataR*caaT*ggtL:1-ggattc*aagR*ggcR*tatF*ggtQ*agaV:2*aaaP:3*accN:1*ttgI*atcL*agaQ:2*cagR:2*cggH:14*atcV:2*gtgR:2*caaS*aacD:1*gatN:3*gaaD:2*atgL:2*atgT:7*gatS*ctgF*cagG:1*cttD:4*attV-tca*catR*gatN*aagN*agaN:3*aaaI:6*cttI:1*actA:8*atcL*accI:5*attM:1*cgtK*tccM*acaS*ggaD:1*gatA*gatE*gctI:5*cagS*cgcK*tacF:1*tcgD:2*gctE:2*ttcY*gaaD:3*gatN:2*tccN:5*tctT:5*agaK:2*gagF*acaE:4*atgI*gttI*aagQ*gatN*gaaD*tcaE*tccD*gtcE*gaaN*gagV*gagN*actL:1-aga:1*gaaL*tctT*cgtE:1*acaK*aatT*ttgA*tcaA*gaaT*ccaT*attL*tctR*gaaH*cagS:2*gtcP+FEQENER*tccQ:1*ttcQ:1*caaA:3*gcaT:4*gtcM+VR*gatN*tttA*tggF*agcV*aatE:2*tctQ*agtL*tttY:1*gctQ*gagQ:1*attQ*cgtQ:1*caaE:1*gagQ:1*gatQ:1*caaE*gctL*ctcQ:4*tatQ*gctQ*gaaL*caaA:1*agaQ*attQ*ttgY*ttcW:4*gtcR:2*caaA:1*cagA:1*cgtQ*attL:2*gagQ:3*cgcL*gagD:1*tacA:1*tcaR*caaH:11*ctgI:1*gctT:1*agaK:3*gagD*caaN:3*ctaM:2*caaS:3*cgtK:1*aagQ:3*actN:1*attL*acaA*gctS:3*tcaT:1*actQ*cagE:1*attL*ggcA:4*ttgQ*attL*caaN*aacL:1*catQ:2*ataV*actE*ctaY:16*cagT:6*aatT:1*ttcS:2*ctcI:11*gacE:6*aagR:1*ctgM:1*tcgA:2*gtgI:3*ggtD:2*cgtK:7*gacE:2*aaaR*gttF:1*ggtN:1*aaaT*ggtK*gatT*gagD*attL*gacQ*aagT:1*gagQ:1*caaE*attK*agaD*gatE:1*accE*ttgQ:2*gatA*gatT*gccL*gagD:1*aacA:2*tcgA:2*tccT*actA:2*gctN:2*aagN:2*atcL*gaaQ*gctE:1*gagQ:1*cagK+FKDVT:1*agaN*cagA*caaP:1*tcgD*gctE*ttcK*aatL*ccaK*gaaQ:2*caaE*ttaQ:1*aagQ*gatE:2*gaaL:1*ttaI:1*attM*ctcM:5*gatE*gcgT*ctgI*gtgK*gaaD:1*gccV:1*gctT:1*aagN*gagS*aatR:1*acaE*gctK*attS:1*gagM:1*atcN*gacK*caaL-gttttggttgat*cacN*ttaS:2*cttV*actL*tccG:1*gttI:4*ctcK:2*ctcI*aagN:1*ttaI:3*ttaV*tatF:10*cagL:6*ttaV:1*tccT*attL*atcL:2*gcgS:1*tccG*tctL*gctV*atgT:2*ggtA*gacS:3*gacN*ttcM*atcL*attV:2*ccaD:1*ggaA*gatN*cacE:1*gagL*attV:1*cgtD:1*ctcI*aatT*gacN:2*tctT:2*gctS:1*gttI:3*tctT:2*attL*gtgT:2*gttT:1*tcaQ:1*gcaD*gagF*gctQ:1*caaD*ttcL:1*gctD:2*agaK*gctD*actI:2*gaaI:2*gaaV*ttcY*ttcL*aaaE:2*cttY*tccH*gatS*agtN:1*gagV*ggcN*gaaK+S*gtgI*gaaD*gagD*aagQ:2*gtgI:1*atgI*gccN:2*ttgI*gcaD:3*aaaI:2*gatT:1*accL:2*actV:1*gacS*ttaM+K*gccT:1*agaS:1*aatK*cttI*ttgD*aacL:1*aacK:1+D*gcgG*aacE:1*ggcS:3*gaaD*actK:1*ttgM*cagE*aatH:1*gccS*gccR*tccV:1*tcaE*aagE:2*tccV*cagH:1*actS:3*tcgN*aaaQ*gatP*caaF:1*ccaV:1*atcM:1*caaS:2*tttI:1*attV:2*gctS:1*gttL*tccA:2*attS:1*gttI*accI*aatT*tccA:1*gaaK*cttY:2*aaaR:1*gctS:1*gctE:6*gcaN*aagN*aacG*aaaR:2*attH:3*gaaS*tacF:3*cacN:1*cgtK:8*gctS:2*gttI:1*cttY:2*aagN*attT:2*gaaR*actI:5*tctQ*gggN*aatK*aaaH:1*catN:1*cagE:1*attV:3*catR:6*gttA:3*gctS:1*tcgA:3*gctS*acgQ:1*ttgM:4*gacN*aatK:2*actD:3*aacK:1*acaN*tctL:2*agaK*agtQ:2*aatD:2*caaN*gacK*attL*ttaI*actI-cagagaagcaac:1*ggtK*agtH*ggtE*gagV*aatE:1*gttI:5*acaS*aatI:5*gtgA:15*atcS*gcaS:10*tatF*ggcS:1*agaK:1*gggD:2*agcD:2 +JAMAIE010000005.1:589225-655853 miniprot mRNA 38513 41626 2775 - . ID=BC000001;Rank=1;Identity=0.5431;Positive=0.7232;Target=MH260578_SLA2 30 1072 +JAMAIE010000005.1:589225-655853 miniprot CDS 38513 41626 2775 - 0 Parent=BC000001;Rank=1;Identity=0.5431;Target=MH260578_SLA2 30 1072 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 195 44 154 + JAMAIE010000005.1:589225-655853 66629 29351 29687 120 339 0 AS:i:161 ms:i:161 np:i:58 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaT*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaK:2*agtL*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS*agaS*aagR*agaH*gggR*cccV*tcaQ*agaK*aagE*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatR:2 +JAMAIE010000005.1:589225-655853 miniprot mRNA 29352 29687 161 + . ID=BC000002;Rank=1;Identity=0.3540;Positive=0.5133;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 45 154 +JAMAIE010000005.1:589225-655853 miniprot CDS 29352 29687 161 + 0 Parent=BC000002;Rank=1;Identity=0.3540;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 45 154 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATa1 181 122 181 + JAMAIE010000005.1:589225-655853 66629 26164 26341 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +JAMAIE010000005.1:589225-655853 miniprot mRNA 26165 26344 171 + . ID=BC000003;Rank=1;Identity=0.5593;Positive=0.7458;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +JAMAIE010000005.1:589225-655853 miniprot CDS 26165 26344 171 + 0 Parent=BC000003;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 26342 26344 0 + 0 Parent=BC000003;Rank=1 +##PAF 5476_sc5314_MTL_A_MTLA1 210 116 197 + JAMAIE010000005.1:589225-655853 66629 26113 26332 105 243 0 AS:i:169 ms:i:158 np:i:49 fs:i:0 st:i:0 da:i:24 do:i:9 cg:Z:14M7I1M1I58M cs:Z::2*gaaD*gcaQ*gttM*atgD*gccD*tcaD*ccaK:2*aatE*aacD*aatD+ILIKGEE:1+K*caaK*caaS:2*cgaK:1*agaQ*catR*attL*tccD*aagN*gaaS*gctT*cgcK*agcE*cttF:3*gcaV:2*actK*actN:1*aatQ:1*accN:1*aagR:2*gcaE*ataL:4*tccH:1*atgV*acaS*gcaL*cttS:1*gtaI:10*accK:1 +JAMAIE010000005.1:589225-655853 miniprot mRNA 26114 26332 158 + . ID=BC000004;Rank=1;Identity=0.4321;Positive=0.6049;Target=5476_sc5314_MTL_A_MTLA1 117 197 +JAMAIE010000005.1:589225-655853 miniprot CDS 26114 26332 158 + 0 Parent=BC000004;Rank=1;Identity=0.4321;Target=5476_sc5314_MTL_A_MTLA1 117 197 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1019 7 1019 - JAMAIE010000005.1:589225-655853 66629 38521 41632 1533 3135 0 AS:i:2456 ms:i:2448 np:i:714 fs:i:0 st:i:0 da:i:15 do:i:12 cg:Z:259M1I6M3I15M7D7M4D5M2I254M6D35M4D11M7D70M1I34M1D25M1I227M4D56M cs:Z::1*gagN*tctL:1*ctcI:1*actA*gccS:1*cgtK:2*acgC*tctT:2*gagA*actA:20*ctcR*tcgE:2*aacH*gctC:2*attL:4*aatS*gatN*gaaD*gttT:1*ttaI:2*gctT:2*accV:9*caaP*tctT*gctC:1*gcaI*gaaG:2*aggK:1*ttgI*aacS:1*atcL*gatE*ggtS:1*gggS:1*tctF*ataS*caaN*ggtN*gatG*ggaT*ttcA*aagA:2*ggtT:7*accF:2*atcE*agaQ:2*cagK:2*cggH*aatD:6*accM:6*atcV:2*gtgR:2*caaS*aacD:1*gatN:2*tatF:1*gccS:4*atgL:7*gatN:3*cttV:2*gcaS*acaS:1*tcaR:1*gatT*aagS*agaE:3*aaaV:2*gcaS:3*cttI*gttI*actA:8*atcL*accI:3*aggK:1*attL:1*cgtK:1*acaS*ggaE:2*gatE*gctV*ttaI*gagA:3*cagD:1*tacF*gagD*tcgV:2*gctR:12*cgcK:3*tctT:1*atcV:6*gagS*acaS*gctP:1*aacI:1*atgQ+D*gttE*aagE:1*gaaP*tcaQ*tccG+FPL:1*gaaR:1*gagR*actS*ccaS*agaV*ccaS*gaaN:1*cgtE*cagE*acaS:1*ttgI-tcagaaccaatttctgaacag:1*actR*gtcS:1*cagN*ttcI:1-caaccaattgct*gcaS:4+AM:2*tttS*tggF*agcV*aatR:2*tctQ*agtE*tttL:1*gctE*gagQ:1*attR*cgtQ*ttgA*caaE*caaE*gagR:1*gatQ:1*caaE*gctL:1*caaE:2*cagM*tatL*gctQ*gaaR*caaD:1*agaA*attR*ttgF*ttcW:1*cagE:2*gtcR:2*caaA*gaaV*cagS:1*cgtE*attL:2*gagS:3*cgcA*gagD:1*tacM:1*tcaR:4*cgcK*gtaL:1*gagQ:2*cgcQ:3*gctT:1*agaG:2*tatH:1*caaR:3*ctaM:1*cagE:7*gccV:2*actN:1*attL*acaR*gctI*accV:2*tcaT:1*actE*cagE:1*attL*ggcA*tccV:1*gatT*gagS*ttgQ*attC*caaS*aacH:1*catE:2*ataV*actQ*ctaY:23*aatG:3*aaaI*ctcV:9*gccS:12*tcgA:2*gtgI:3*ggtD:2*cgtK:9*gagA*aaaR*gttI:2*ggtN*aaaD*ggtS*gatE*gagQ*attV*gacQ*aagM:1*gagR*ttgT:1*attL:2*ctcM*accE*ttgT:2*gatK*gatS*gccL:1*agaE*aacT*caaH:1*tcgQ:2*tccA*actS*attL:1*gctS:2*aagS:4*gctE*ttgI-gagaaccagttaagacag*caaK*tctA:2*ttcS*aatE*ccaL*gaaK*ttgE*gaaG*caaG:1*ctaN*aagE*gatE*aagA*gatQ*gaaQ:1*ttaV*gaaQ*attM:3*acaA:3*gcgT*ctgM*gtgQ:3-aaagctcagaag:1*aatQ*gaaH*acaQ:3*gagK*cagE*atcA*gacE-caagttttggttgatcactta:1*aagR*cttV*actL*tccR:1*gttI:1*gctT*atcL:1*ctcK*agtV*ggcS*ctcS*aagD:2*caaR*gacE*gccF*ttaV*tatF*gaaN:1*gacE:2*atgV*caaY*gctS*ggtN*aacT*cagD*aacI*tcaP*tcgT*cctA:3*ctcG*tccT*attM*atcL:1*aaaR*gcgV:1*tccH*tctL*gctS*atgT*gacQ:1*ggtS*gacT*tctT*tttM*aatT:1*ttcY:1*attA:1*gggN*ccaM*aatK:1*gatE+S*cacE*gctS*gagG*attL:1*cgtK*tctA*ctcI*aatI*gacE*ttcL:1*tctD:2*gctG*gacN*gttL*ttaF*ttaS:1*tctV:1*ggtA*attV*gtgL:1*cttD*gttR:1*tcaD:1*gcaV*gagQ-gct*gatE*caaI*ttcL*ataV*gctE*actG*gcgS*agaK*gctD*actL:1*gagH*gaaM*tcgV:1*gaaL:2*aaaE:1*ttgM:2*gatN*agtH+L:1*gagK*ggcL:1*gtgI*gaaH:1*aagR:2*gtgR:1*atgI*gccT:2*ttgI*gcaN:3*aaaV*cttI:1*gatS:1*accM:1*ttgV*actV:1*gacV*ttaF*gccR*ccaD*agaP*tctG*aatR*cttK*ttgT*aacP*agcV*aacS*gttS*gcgD*aacE:2*gacY*cttF*gtgL*gaaD*actK:2*cagA:1*gcgT*gccQ:1*tccA*attV*tcaE:2*tcaA*tccG*cagL:1*actE:1*ctaM*ctcS*tcgT*aaaR*gatH*caaK*gacL*ccaE*tctV*atcQ*tcaE*caaD*atcG*gacS*tttL:1*attV:1*aaaE:2*gttI:1*gctC:1*attS:1*gttI*accI:1*tccA*attV*gaaK:2*attL*aaaE:1*gctC:1*gctD*tcaA:5*gcaS*aagR*aacG*aaaR:2*attM:1*aagR*actA*gaaL*tacF:3*cacN:10*gctS:1*tctQ:2*cttY*tccA:1*aagG:3*gaaR*actM:5*tctA:1*aatT*aaaN:1*catS:1*cagE:5*catN:3*gctS*tcaA:1*gttA:3*gctS*gctS:4*gctS*acgD*tttL*ttgM:1*aagQ*actS*caaH*gacL:3*actE:2*aagR*aacK:3*gccS:1*agaK*agtM:2*aatT*caaK:1*caaR*gacS*attL:1*actL-cagagaagcaac*ggcS*ggtD*agtE*ggtS*gagN*aatK*gatT:4*aaaQ:2*aatA:3*aaaR:1*gtgA:1*atgL:10*aatS:2*atcS*gcaH:10*tatF*ggcS:3*gggE*gacE:1 +JAMAIE010000005.1:589225-655853 miniprot mRNA 38522 41632 2448 - . ID=BC000005;Rank=1;Identity=0.4890;Positive=0.6833;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +JAMAIE010000005.1:589225-655853 miniprot CDS 38522 41632 2448 - 0 Parent=BC000005;Rank=1;Identity=0.4890;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 187 128 187 + JAMAIE010000005.1:589225-655853 66629 26164 26341 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +JAMAIE010000005.1:589225-655853 miniprot mRNA 26165 26344 171 + . ID=BC000006;Rank=1;Identity=0.5593;Positive=0.7458;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +JAMAIE010000005.1:589225-655853 miniprot CDS 26165 26344 171 + 0 Parent=BC000006;Rank=1;Identity=0.5593;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 26342 26344 0 + 0 Parent=BC000006;Rank=1 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 190 41 151 + JAMAIE010000005.1:589225-655853 66629 29351 29687 126 339 0 AS:i:172 ms:i:172 np:i:59 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaS*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaN:3*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS:1*aagR*agaH*gggR*cccV*tcaQ*agaK*aagQ*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatI:2 +JAMAIE010000005.1:589225-655853 miniprot mRNA 29352 29687 172 + . ID=BC000007;Rank=1;Identity=0.3717;Positive=0.5221;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 42 151 +JAMAIE010000005.1:589225-655853 miniprot CDS 29352 29687 172 + 0 Parent=BC000007;Rank=1;Identity=0.3717;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 42 151 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 294 0 294 - JAMAIE010000005.1:589225-655853 66629 16016 16892 504 882 0 AS:i:856 ms:i:856 np:i:214 fs:i:0 st:i:0 da:i:72 do:i:0 cg:Z:98M1I99M1I95M cs:Z:*gccM:1*gctS*aaaD*accK*ataS:1*gccK*accS*aagI*accA:1*accS*accA*aagN*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctK*ttaV:1*aagG*ccaD:2*tttV*cagS*atgL:2*tcgK:1*ctgI:1*gcaT*gatE:1*attV*acaL*ggtA*atcA:1*tctA:2*tctT:1*ggcS:8*ctgT*accA:3*gccV:2*tacK:3*agtI*ttcM*gttT+D:1*gccT*gatR*aagG*atgQ*agtA:1*atgT*gctF*tatQ:2*cccA*atcA:2*gttI*agtA:5*atcV:4*tctA:1*attV:8*actN*ggtS:2*atcE*gaaS:3*aacH:2*aatH:1*tttL:2*gtgL*gccL*cgaK:1*gtgT*actR:2*ggtN*cctL*agtT:6*tctG*acaP:2*gtgA:2*gttI:3*tcaA:4*actS:2*ttgV:2*gaaK*atgL:1*atcV+E*tccN*ttcL*ggaS:1*aaaD*aagP*gagK*tccT*ttcK*ttcA:1*tttH:2*tctA:2*cttI:3*gccV:3*attV:13*gcaS:3*aacG*catQ:1*gtgS*gtgF*cacG:2*actK*aaaD:1*ttgI*aagS*gacR:2*cccL*agcG:6*gtgT:1*agtA:1*gtgI:4*aatH:2*atcL*attT:1*ctcI*gttA*gtaL:3*aagR:1*atgL*agaK:3 +JAMAIE010000005.1:589225-655853 miniprot mRNA 16014 16892 856 - . ID=BC000008;Rank=1;Identity=0.5714;Positive=0.7279;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +JAMAIE010000005.1:589225-655853 miniprot CDS 16014 16892 856 - 0 Parent=BC000008;Rank=1;Identity=0.5714;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 16014 16016 0 - 0 Parent=BC000008;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 305 7 305 - JAMAIE010000005.1:589225-655853 66629 16016 16895 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gacK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +JAMAIE010000005.1:589225-655853 miniprot mRNA 16014 16895 818 - . ID=BC000009;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 8 305 +JAMAIE010000005.1:589225-655853 miniprot CDS 16014 16895 818 - 0 Parent=BC000009;Rank=1;Identity=0.5537;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 8 305 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 16014 16016 0 - 0 Parent=BC000009;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1045 0 1045 - JAMAIE010000005.1:589225-655853 66629 38521 41641 1827 3165 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D54M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gaaS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE*agtE +JAMAIE010000005.1:589225-655853 miniprot mRNA 38522 41641 2946 - . ID=BC000010;Rank=1;Identity=0.5773;Positive=0.7488;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +JAMAIE010000005.1:589225-655853 miniprot CDS 38522 41641 2946 - 0 Parent=BC000010;Rank=1;Identity=0.5773;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1044 0 1044 - JAMAIE010000005.1:589225-655853 66629 38524 41641 1827 3162 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:15 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D53M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gaaS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE +JAMAIE010000005.1:589225-655853 miniprot mRNA 38525 41641 2946 - . ID=BC000011;Rank=1;Identity=0.5778;Positive=0.7495;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +JAMAIE010000005.1:589225-655853 miniprot CDS 38525 41641 2946 - 0 Parent=BC000011;Rank=1;Identity=0.5778;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 305 7 305 - JAMAIE010000005.1:589225-655853 66629 16016 16895 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gacK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +JAMAIE010000005.1:589225-655853 miniprot mRNA 16014 16895 818 - . ID=BC000012;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 8 305 +JAMAIE010000005.1:589225-655853 miniprot CDS 16014 16895 818 - 0 Parent=BC000012;Rank=1;Identity=0.5537;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 8 305 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 16014 16016 0 - 0 Parent=BC000012;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 305 7 305 - JAMAIE010000005.1:589225-655853 66629 16016 16895 495 894 0 AS:i:824 ms:i:818 np:i:213 fs:i:0 st:i:0 da:i:69 do:i:0 cg:Z:16M2I89M2I102M1I86M cs:Z::1*gccN*aacD*gctD*aaaS:1*ataM*acgQ*gccY*accE*aagR*accI:1*accP*accA:1+GV*tggF:6*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctS*ttaQ:1*aagG*ccaQ*tcgN:1*tttL*cagT*atgI:1*ttcI*tcgR:1*ctgI*aaaS*gcaS*gatE:2*acaL:1*atcA:6*ggcS:8*ctgT:4*gccI:2*tacA:2*gagD*agtY*ttcL*gttT*ccaK:1*gatN*aagN*atgN*agtQ+DL:1*atgT:1*tatI:3*atcC:2*gttL*agtA:1*gctG*gttI:2*atcL:6*attV:8*actS*ggtS:2*atcK*gaaP*caaA:4*aagR:2*tttL*gatH:1*gtgL:2*attM*gtgA*actK:3*cctL*agtG*gccS*ctaW:4*tctL*acaP:2*gtgT:6*tcaA:4*actS:2*ttgV:2*gaaK*atgF:1*atcV*tccK*ttcK*ggaL*atgS*aaaF*aagD:1*tccT*ttcT+R*ttcA:1*tttH:2*tctA:2*cttM:3*gccV:3*attV:6*ctcV:6*gcaS*tccA*ggaE*acaK*aacH*catQ:1*gtgI*gtgI*cacT*attV:2*aaaT:1*ttgM*aagR*gacK:3*agcG:1*atgL:4*gtgF:3*gtgI:4*aatH:2*atcL*attT:1*ctcV*gttA*gtaL:1*gagQ:1*aagR*cgtT*atgW:2*ggtA*ttaM +JAMAIE010000005.1:589225-655853 miniprot mRNA 16014 16895 818 - . ID=BC000013;Rank=1;Identity=0.5537;Positive=0.7148;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 8 305 +JAMAIE010000005.1:589225-655853 miniprot CDS 16014 16895 818 - 0 Parent=BC000013;Rank=1;Identity=0.5537;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 8 305 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 16014 16016 0 - 0 Parent=BC000013;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1044 0 1044 - JAMAIE010000005.1:589225-655853 66629 38524 41641 1827 3162 0 AS:i:2946 ms:i:2946 np:i:790 fs:i:0 st:i:0 da:i:6 do:i:15 cg:Z:216M1I51M2D15M2D13M3I6M1I8M3I261M5I182M1I31M2D8M1I34M1D151M3D53M cs:Z:*aagM*acgS*atcR*tctQ:1*tctA:2*cagK*actV*gccS*cttI*cgtK:2*acgC:1*gctT*gatE:1*actA:12*tacF:4*aagR:2*ctcK*tcgA:1*tggY:1*gctG:1*aagR*attL:7*gttI*cagP:3*gctS:12*caaP:2*ctcI*gcaK:2*tacI*aggK:1*ttgR*aacD:2*gatQ*ggtS:2*agaH*tctV*ataF*caaP:3*ttcM:1*ggcR:5*aaaR:2*gtcD*accR:5*cttI*cagD:2*cggN*aatS:1*cgtK:11*atcV:2*gtgK:2*caaS*aacD:1*gatN:10*tccV:4*cttI*gacN:5*attL:2*acaS:1*tcaD*catS*gatS*aagS*agaH:2*tgcL:8*gttI*actA:5*tatF:8*gcaG*attM*tacC*cgtH:1*acaL:1*tctH:2+E:2*gagQ:2*aagI*cagE:1*tacF*gagI:2*catY*gctS*cgtD:1*ttcY*gaaD:3*gatE:2*tccY*attV*cgcK:5*atcV*acaN:2*agaK:2*gagF*acaE:2*aacD:1*atgF*gttN*aagW:2*tcaT-tccgtc*gaaD:1*gagL*actK:2*ccaI*gaaL*tctA*cgtE*cagE*acaP*aatS:1*tcaQ-gaacca*attV:1*gaaS*cagK*ccaA*actP*gtcS*tccE*cagE:1*cagG*caaT:1+TEV:1*gctH*gcaS:3+A:1*gtcT:1*tttL:1*agcA*aatQ*cagS+NAL*caaK*tctQ*agtQ*tttY:1*gctQ:2*attQ*cgtL:1*caaE:3*gatQ*gcaQ:1*gctL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*attE*ttgY*ttcW:1*cagK:1*cagK*gtcE*caaL:1*caaE*gaaQ:2*cgtL*attY:2*gagQ:2*cttE*cgcS*gagD:1*tacL:1*tcaR:1*gccV:4*gctG:7*gctI:4*tatF*gagD*caaR:3*ctaM:1*cagR:3*cagE:8*attL*acaS*gctS:3*tcaN:1*actQ:2*attL:1*tccA:1*gatE:2*attL*caaG:1*ttgI*catQ:2*ataA*actA*ctaQ:2*aacS:2*gagD:10*cagE:6*aatS:3*aaaT*ctcA:1*caaL:2*gcaS:2*caaR:8*ttgI:1*aagR:3*tcgA:2*gtgI:3*ggtD:13*aaaR*gttS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gacE*aagR:2*ttgA*caaR*attL:1*gatE*ctcQ*accE*ttgT:2*gatA:1*gccI:1*agaK*aacL*caaH:2*aacE:1*tccK*actS*attF*tttL*gctS:2*aagN:4*gctE:1*gagQ:3*agaQ*cagA*caaS:1*tcgL*gctS*ttcE*aatS*ccaA*gaaN+ERLKA:2*caaA*ttaQ:1*aagQ*gatE:2*gaaD:2*gaaA:1*ctcA*caaH:3*gacE*gatA*gcgT*ctgI*gtgK:2*gccT*aaaG*gctQ:1*aagS*gagI*aatQ*gaaN*acaS:2*gaaD:4*caaA*gttI:1*gttL*gatE:1*ttaI*acgD:3*tccE*ttgV*gttI:4*ctcT:2*ctcV*aagQ:1*ttaI:2*gccG:1*tatF:18*ctcS:5*gcgS:1*tccN*tctL:1*atgT*gacE:1*ggtT*gacS:3*gacG:2*attA:7*gctV*gagS*attV:1*cgtQ*tctT*ctcI*aatT*gacE:2*tctT:2*gctV:1*gttL:3*tctC:2*attV:3*gttA*aaaS:2*gcaQ:2*gatE*caaA*ttcL*ataL*gctN*actS:2*gctD*actV:5*gaaS:1*ttcI*aaaV:2*cttY:1*gatS:2*gagK:1+L*gaaS*gtgD*gaaQ:4*gtgK*gtcA*atgI*gccE:2*ttgV*gcaD*gtgI:1*gagQ*aaaE:2*gatT:1*accS*caaS:1*actV:1*gacK:1*gccI-ccaaga:1*aatS*cttQ*ttgV:1*agcI:2+N*gcgD:1*ttgV:1*gacE:2*gaaD*actR:1*ttgM*cagA*aatH:2*gccE*tccT:1*tcaA:3*tccD*cagH:2*tcgD:2*tcgT*aaaN*gatA*caaK*gacQ-cca*tctD:2*caaT:2*tttM*gagK:2*aaaN*gctS:1*gttL:7*aatK*tccA:1*gaaI:4*gcaT:1*attT*gctD*tcaC:1*gagN:3*gcaN*aagH*aacG*aaaR:2*attS:1*aagR:1*gaaS*tacF:5*cgtK:8*gctS:4*cttY:2*aagN*attS:2*gaaQ*actI:6*gggS*aatT*aaaS:1*catP:6*tcaC*catN*gagD:5*gttA:10*acgS:1*ttgM:5*aatK:2*actL:3*aacS:9*aatR:3*gacE:2*actS:1*agaK*agcD-aacggcggt*agtK*ggtE:1*aatL*gatE*gttI:5*acaS*aatA:6*gagQ:11*aatT:2*atcQ*gcaN:2*aaaR:6*aagR*tatF:2*agaH*gacA*gggD*gacE +JAMAIE010000005.1:589225-655853 miniprot mRNA 38525 41641 2946 - . ID=BC000014;Rank=1;Identity=0.5778;Positive=0.7495;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +JAMAIE010000005.1:589225-655853 miniprot CDS 38525 41641 2946 - 0 Parent=BC000014;Rank=1;Identity=0.5778;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 287 14 287 - JAMAIE010000005.1:589225-655853 66629 16016 16829 477 819 0 AS:i:799 ms:i:799 np:i:201 fs:i:0 st:i:0 da:i:135 do:i:0 cg:Z:77M1I99M1I95M cs:Z::1*ttcA*ggaA*ggtS*gctM*gtaF:2*ttcL*tgcF:14*cctK*ttaV:1*aagG*ccaD:2*tttV*cagS*atgL:1*ttcY*tcgK:1*ctgV:1*gcaT*gatE:1*attV*acaL*ggtA*atcA:1*tctA:4*ggcS:8*ctgT*accA:3*gccV:2*tacK:2*gagG*agtI*ttcM*gttT+D:1*gccT*gatK*aagG*atgQ*agtA:1*atgT*gctF*tatQ:2*cccA*atcA:2*gttI*agtA:5*atcV:4*tctA:1*attV:8*actN*ggtS:2*atcE*gaaS:3*aacH:2*aatH:1*tttL:2*gtgL*gccL*cgaK:1*gtgT*actR:2*ggtN*cctI*agtT:6*tctG*acaP:2*gtgA:2*gttI:3*tcaA:4*actS:2*ttgV:2*gaaK*atgL:1*atcV+E*tccN*ttcL*ggaS:1*aaaD*aagP*gagK*tccT*ttcK*ttcA:1*tttH:2*tctA:2*cttI:3*gccV:3*attV:13*gcaS:3*aacG*catQ:1*gtgS*gtgF*cacG:2*actK*aaaD:1*ttgI*aagS*gacR:2*cccL*agcG:6*gtgT:1*agtA:1*gtgI:4*aatH:2*atcL*attT:1*ctcI*gttA*gtaL:3*aagR:1*atgL*agaK:3 +JAMAIE010000005.1:589225-655853 miniprot mRNA 16014 16829 799 - . ID=BC000015;Rank=1;Identity=0.5824;Positive=0.7363;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +JAMAIE010000005.1:589225-655853 miniprot CDS 16014 16829 799 - 0 Parent=BC000015;Rank=1;Identity=0.5824;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 16014 16016 0 - 0 Parent=BC000015;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 195 44 154 + JAMAIE010000005.1:589225-655853 66629 29351 29687 120 339 0 AS:i:161 ms:i:161 np:i:58 fs:i:0 st:i:0 da:i:174 do:i:189 cg:Z:66M1I19M1D14M2D10M cs:Z::1*tccH:2*aagQ:2*aagT*cttT:1*gccP*gatL*tatL*gaaK:1*tttL*attL*gaaT*ggaE*ctaY*aagG*gatA*catI*aaaD*gctN:1*caaK:2*agtL*acgN*tacK*atcV*cgcA*caaP*catK*ttgK*aggS*agaS*aagR*agaH*gggR*cccV*tcaQ*agaK*aagE*cggK*acaK*aagR:6*tatF:3*ttcY:3*gttI:1*acaN+Y*gatN*caaS:1*ggaK*gtcA:2*tctQ*caaD:2*aaaD*gaaA:1*gagN:1*aatE*ccaK*gaaH-atc:1*cttQ*gtaI:1*aagS*agaL:1*tccA*acaI*gagQ:2*ttgH:1-gttttg*agtK*gatS*cgcK*cttE:2*ttcS*gatR:2 +JAMAIE010000005.1:589225-655853 miniprot mRNA 29352 29687 161 + . ID=BC000016;Rank=1;Identity=0.3540;Positive=0.5133;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 45 154 +JAMAIE010000005.1:589225-655853 miniprot CDS 29352 29687 161 + 0 Parent=BC000016;Rank=1;Identity=0.3540;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 45 154 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 181 122 181 + JAMAIE010000005.1:589225-655853 66629 26164 26341 99 177 0 AS:i:171 ms:i:171 np:i:44 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:59M cs:Z::2*cgaK:4*tccP*aagE*gaaS*gctS*cgcK*agcE:6*gaaK*actV*actK*aaaR*aatF:1*accN*agaS:3*gcaE*ataR:2*gagR*aaaE*tccC:1*atgI*acaS*gcaP:11*atgA:1*accS:1*accS:1*ataA +JAMAIE010000005.1:589225-655853 miniprot mRNA 26165 26344 171 + . ID=BC000017;Rank=1;Identity=0.5593;Positive=0.7458;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +JAMAIE010000005.1:589225-655853 miniprot CDS 26165 26344 171 + 0 Parent=BC000017;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 26342 26344 0 + 0 Parent=BC000017;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 440 0 440 - JAMAIE010000005.1:589225-655853 66629 38521 39883 591 1371 0 AS:i:863 ms:i:863 np:i:295 fs:i:0 st:i:0 da:i:975 do:i:12 cg:Z:15M4D11M7D70M1I34M1D25M1I224M1I7M5D51M cs:Z:*gaaQ*attM:3*acaA:3*gcgT*ctgM*gtgQ:3-aaagctcagaag:1*aatQ*gaaH*acaQ:3*gagK*cagE*atcA*gacE-caagttttggttgatcactta:1*aagR*cttV*actL*tccR:1*gttI:1*gctT*atcL:1*ctcK*agtV*ggcS*ctcT*aagD:2*caaR*gacE*gccF*ttaV*tatF*gaaN:1*gacE:2*atgV*caaY*gctS*ggtN*aacT*cagD*aacI*tcaP*tcgT*cctA:3*ctcG*tccT*attM*atcL:2*gcgV:1*tccH*tctL*gctS*atgT*gacQ:1*ggtS*gacT*tctT*tttM*aatT:1*ttcY:1*attA:1*gggN*ccaM*aatR:2+S*cacE*gctS*gagG*attL:1*cgtK*tctA*ctcI*aatT*gacE:2*tctD:2*gctG*gacN*gttL*ttaF*ttaS:1*tctV:1*ggtA*attV*gtgL:1*cttD*gttR:1*tcaD:1*gcaV*gagQ-gct*gatE*caaI*ttcL*ataV*gctE*actG:1*agaK*gctD*actL:1*gagH*gaaM*tcgV:1*gaaL:2*aaaE:1*ttgM:3*agtH+L:1*gagK*ggcL:1*gtgI*gaaH:1*aagR:2*gtgR:1*atgI*gccT:2*ttgI*gcaN:3*aaaV*cttI:1*gatS:1*accM:1*ttgV*actV:1*gacV*ttaF*gccR*ccaD*agaP*tctG*aatR*cttK*ttgS*aacP*agcV*aacS*gttS*gcgD*aacE:2*gacY*cttF*gtgL*gaaD*actK:2*cagA:1*gcgT*gccQ:1*tccA*attV*tcaE:2*tcaA*tccD*cagL:1*actE:1*ctaM*ctcS*tcgT*aaaR*gatH*caaK*gacL*ccaE*tctV*atcQ*tcaE*caaG*atcG*gacS*tttL:1*attV:1*aaaE:2*gttI:1*gctC:1*attS:1*gttI*accI:1*tccA*attV*gaaK:2*attL*aaaE:1*gctC:1*gctE*tcaA:5*gcaS*aagR*aacG:3*attL:1*aagR*actA*gaaL*tacF:3*cacN:10*gctS:1*tctQ:2*cttY*tccA:1*aagG:3*gaaR*actM:5*tctA:1*aatT*aaaN:1*catS:1*cagE:5*catN:3*gctS*tcaA:1*gttA:3*gctS*gctS:4*gctS*acgD*tttL*ttgM:1*aagQ*actS*caaH*gacL:3*actE:2*aagR*aacK:3*gccS:1*agaK*agtM:2*aatT*caaK:1*caaR*gacS+L*attL:2*cagD*agaE:2-ggcggtagtggtgag*aatK*gatT:4*aaaQ:2*aatA:3*aaaR:1*gtgA:1*atgL:10*aatS:2*atcS*gcaQ:10*tatF*ggcS:3*gggE*gacE:1 +JAMAIE010000005.1:589225-655853 miniprot mRNA 38522 39883 863 - . ID=BC000018;Rank=1;Identity=0.4311;Positive=0.6455;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +JAMAIE010000005.1:589225-655853 miniprot CDS 38522 39883 863 - 0 Parent=BC000018;Rank=1;Identity=0.4311;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 671 0 671 - JAMAIE010000005.1:589225-655853 66629 38512 41641 825 2121 0 AS:i:1065 ms:i:1065 np:i:398 fs:i:0 st:i:0 da:i:6 do:i:3 cg:Z:94M2I6M10I3M2I75M7I2M1I86M34I14M1I9M9I9M1I24M6I19M3D8M1I21M2I39M22D45M7D12M1D25M1I15M1I13M2D12M1I5M2I21M1D4M1260N7M3I19M cs:Z:*aagM*acgS*atcR*tctE:1*tctQ*gatT:1*cagE*actK*gccS*cttI*cgtK:2*acgL:1*gctG*gatG*gagD*actV:20*ctcK*tcgT:1*tggF:1*gctL:2*attL*ttgQ:1*attY*cagA*aatT:2*gttL:6*atcV*accL*atcL:8*caaP:3*gcaV:2*tacI:2*ttgI*aacD:2*gatE*ggtS:1*gggS:1+IF:1*ataV*caaD*ggtS:1*ggaD+GSKYGSSRMR*ttcY*aagI:1+DE:1*ggtA*agaK*ttgA:1*aaaG:3*accF*tacF:1*atcV*agaE:5*cggK*aatS:1*cgtK:4*accI:6*atcV:3*actS:1*caaS*aacD:1*gatN:4*gccT*atcV*atgL:3*tccN:1*caaE:1*tcaV*cttA:1*gatN:2*agaK:4*acaG:2*catN*gatR:1+SGFGSFS*agaS*tctG+L*gaaD:1*aaaR*attG*tcaA*gcaV:1*gtcI:3*actS:1*tctT:3*tatF:2*atcV:3*ttgM*aggK:1*attL:1*cgtK*tccQ*acaM:1*tctD*gatY:3*gagS*ccaG:1*aagF*cagD:2*gagT*tcgE:1*catC:3*ttcN:3*gctF:2*tccN*tccT*attV*cgcQ*tacP:1*actK*tctN:5*agaT:2*gagS*acaD*gctP*cctM*aacS*ctcR*atgI*gttI*aagD:2+DDAEVNDVVFEKRSKSSVSSLPSRTPTRENLRDP:1*tccP:1*gaaH:1*gagA*actP:1*agaV*ccaA*gaaR*tctP*cgtA*cagE+V*acaR*aatS:2*gaaQ:1*attA*tctA*gaaP+VVPIATGVV*cagM:1*actV:1*tccT*cagG*ttcY*cagS:1+P:1*attL*gctQ*gcaV*caaV:3*gtcA*gatE:3*aatI*cagP*caaS*tctT:1*tttA*gagP*gctT*gagR*caaS*attV+VASHDD*cgtL:1*caaI*caaE*gagQ*cgtQ*gatK*gcaQ:1*gctQ*ctcI:6*gaaL:1-caaagaatt:1*ttcE*gaaH:1*cagK:1*gtcL:1+L:5*cgtA*attL*gcaL:1*gagQ*caaR*ttgI*cttQ*cgcQ*gagQ*cagE*tacM*cagE*tcaK*caaE:1+LI:1*ggcQ*cgcK*gtaT:1*gagS:1*gagQ*cgcN:1*ctgI*ctgM*gctS*ttgF*agaK*ggaN:3*caaR:2*cttV*ctaM:1*cagE:3*cagK:2*aagQ*gccK:2*actS:1*attL-acagctaccactcaatcagctactcagcaaattggctccaaggatgagttgattcaaaacttgcat:1*caaS*ataV*actK*ctaE*tggF:1*aacE:2*gagQ:15*ttgI:1*aatK:1*ttcM:2*ctcT:1*caaD*aaaD*gccL:1*tctE:1*caaE*gaaS*gccE*attV-gacaagcgtgagaaattggaa:1*gacK:3*aagS*aacA*gtgL*gaaS*ttgN*gctV:1-cta:2*gaaN*cgtN*gacN*cgtK*gctG*cgtE*ttgS*gacE*ttgR:1*aaaE*gttA:1*ggtD*ggtR*aaaS:1*gatM*gagI*attL*gacK*aagS:1+V:1*ttgS*caaS:1*agaK*gatS:1*accQ*ttgD*aaaP*ttaI*gatA*gatS*gccL*gagD+V*agaS*aacD*caaL*tcaA*tcgT*aacD:2*actN*attA:1*gctI*caaD-cacaag*aaaG*gagD:1*gaaN*gctT*ttgI*gagV:1*cagI:1*agaP*cagE+F*caaV:1*tcgL*gctL*ttcL+RC*aatP*ccaQ:1*ttgM*gaaQ*caaT*ttaI*ctaA*aagE:1*aagF*gatL*gaaR*gagC:1*gaaS*attS*ctcA*caaK:2-atg:1*gatS*gcgV*ctgI~gt1260ag:2*ctaN:2*gcaF*agaQ+EKL:7*aagQ:1*ggcS:2*gacY:3*agcD:1*gaaV +JAMAIE010000005.1:589225-655853 miniprot mRNA 38513 41641 1065 - . ID=BC000019;Rank=1;Identity=0.3890;Positive=0.5629;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 671 +JAMAIE010000005.1:589225-655853 miniprot CDS 39851 41641 995 - 0 Parent=BC000019;Rank=1;Identity=0.3791;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 642 +JAMAIE010000005.1:589225-655853 miniprot CDS 38513 38590 70 - 0 Parent=BC000019;Rank=1;Identity=0.6207;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 643 671 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 228 111 211 + JAMAIE010000005.1:589225-655853 66629 26035 26332 117 306 0 AS:i:157 ms:i:157 np:i:56 fs:i:0 st:i:0 da:i:180 do:i:9 cg:Z:29M2D12M3I56M cs:Z::1*aacD*tgtV*tcaK:1*ataE:2*tctM:1*catF*tctD*cctT*ccgE*gaaH*gcaE*tcaC*tctT*atgD*aatT*actS*tctE*aatD*gtcI*tgtS*tctD*gttK:2-gcagtt*atgI*gccS:1*ccaT*gacN:1*aatD:1*aatP:2*caaN+CYP*aagT*aagY*cgaK:1*agaS*catF:1*tccK*aagH:1*gctS:1*agcG*cttI:3*gcaI:1*gaaK*actV*actK*aaaQ*aatC:1*accN*agaT*aagS:2*gcaL*ataY:2*gagQ:1*tccL*ggtD*atgL:1*gcaP*cttS:3*gtgI:8*accA:1 +JAMAIE010000005.1:589225-655853 miniprot mRNA 26036 26332 157 + . ID=BC000020;Rank=1;Identity=0.3824;Positive=0.5490;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 112 211 +JAMAIE010000005.1:589225-655853 miniprot CDS 26036 26332 157 + 0 Parent=BC000020;Rank=1;Identity=0.3824;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 112 211 +##PAF 4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 212 51 212 + JAMAIE010000005.1:589225-655853 66629 29351 32111 153 507 0 AS:i:137 ms:i:166 np:i:82 fs:i:0 st:i:0 da:i:174 do:i:0 cg:Z:14M4I19M3I31M1I21M1D18M2D8M2295N4M6I11M5D21M cs:Z::1*tccE:2*aagS*ataL:1*aagT*cttP:1*gccI:1*tatL*gaaQ+EILI:2*attN*gaaQ*ggaA*ctaT*aagR*gatN*catC*aaaA*gctG*ggtD*caaS*gatS*agtW*agtG*acgS*tacQ*atcL+FDM*cgcK*caaV*catP*ttgT:1*agaK*aagS:1*gggI*cccR*tcaC*agaK:1*cggT*acaR*aagC:6*tatF:3*ttcY:2*aacS*gttI+Y*gcaN*acaV*gatE*caaH:1*ggaR*gtcE:2*tctT*caaL:1*gcgG:1*gaaL:1*gagK*aggN*aatE:1*gaaN-atc*caaK*cttA*gtaI:1*aagN:2*tccA*acaI:3*ttgS*tctQ*gttA*ttgI*agtN*gatQ-cgcctt*cccD:1*ttcV:3*ttgF*cttK~gt2295ag*gtaA:1*caaG*ataL+EVETFP*ttaI:1*ttcY*tctT*aagS:1*attV*agtR:1*cgtE:1-cttcgccaactatat*ctcW*tccT*tcgF:2*acaR*gtcN*aaaN*ttcE:1*attA*cttV:1*tccD*agcI*tccY*tttY:3*ctgV +JAMAIE010000005.1:589225-655853 miniprot mRNA 29352 32114 166 + . ID=BC000021;Rank=1;Identity=0.3018;Positive=0.4852;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 52 212 +JAMAIE010000005.1:589225-655853 miniprot CDS 29352 29693 141 + 0 Parent=BC000021;Rank=1;Identity=0.3197;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 52 170 +JAMAIE010000005.1:589225-655853 miniprot CDS 31989 32114 25 + 0 Parent=BC000021;Rank=1;Identity=0.2553;Target=4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT 171 212 +JAMAIE010000005.1:589225-655853 miniprot stop_codon 32112 32114 0 + 0 Parent=BC000021;Rank=1 +##PAF 1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1064 0 1064 - JAMAIE010000005.1:589225-655853 66629 38515 41650 1773 3210 0 AS:i:2844 ms:i:2844 np:i:773 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:101M1D163M2D41M1D6M12I259M9I24M3I159M1I230M2D56M cs:Z:*gtgM*atgA*tccT*aagL*acgS*atcK*tctA:1*tctA*gatE:1*cagT*actV*gccH*cttI*cgtK:3*tctN*gctP*gatE:10*gcgQ:2*gttI:4*cacN*aagH:1*tcaG*ctcR*tcgA:3*gctG*ttaM:2*ttgQ:1*attL:6*ttaV:15*caaP:1*gctV:1*gcaR:1*ggtA*tacQ*aggQ:1*ttgR*aacS:1*atcL*gatE*ggtS:1*gggA:1*tctG*ataA*caaH*ggtA-gat:1*ttcR*aagH*ggcT:1*ggtA*agaK:2*aaaY:3*accG*tacL:1*atcL*agaK:2*cagN:2*cggK:2*cgtP*ggtD:13*gtgR*actN*gtcI*caaD*aacD:1*gatN:3*gaaQ:2*atgV:3*tccD:3*tcaA*cttI:1*gatN*ctgF:4*ttcI:1*acaS*attL*tcaK:1*gatG*aagR*agaP*tctN:2*aaaR:4*gtcI:3*actA:6*aagR:1*atcT:1*tcgT:4*attM*tacH*cgtA*tccT:1*ggaN*tctD:7*aagR*cagS:2*gagY:3*gctQ:2*ttcL:3*gctY:4*attL*cgcK:4*ttgV:4*agaT:1*ccaS*gagQ*acaD*gctP:3*atgY-gttaag*gatG:1*tcaN*tccP*gtcE:1*gagG:1*actA:1*agaA*ccaL*gaaP*tctK:1*cagP*acaI*aatS*ttgE*tcaV*gaaA*ccaS*attL*tctA*gaaT*cagG*ccaA:1*gtcT*tccA*cagP*ttcS*cagT*caaT*ccaD*attL*gctT*gcaP*caaE:1*actV-ggt:5*aatT+PQGQQQLQQQQL:2*tctQ*agtQ*tttY:1*gctE:2*attR:2*caaA*caaA*gagQ:1*gatE:1*caaE*gctL*ctcQ:3*cagM*tatL*gctH*gaaL:2*agaQ*attQ*ttgQ:2*cagE:2*gtcR*caaM:1*caaA:4*attL:3*caaA:3*gagD:3*tcaR:2*cagD:2*gtaA:6*ctgI:1*gctN:6*caaR:3*ctaM:1*cagE:3*cagR:3*gccG:2*actG:1*attL*acaQ*gctQ*accV*actI*caaA*tcaN*gctT*actN*cagL:1*attS*ggcQ:3*gagD*ttgM:1*caaR*aacS:1*catQ:2*ataV*actN*ctaS*tggL:1*aacS:2*gagD*agtV:13*aacD*ttgK:1*aatK*aagE*ttcL:1*aaaL:1*caaK*caaV:9*gacE:6*aagR:1*ctgI:1*tcgA:2*gtgI:3*ggtD:9*ttgY:2*gagD:1*gttS*aagR:1*ggtN*aaaQ*ggtK:1*gagV:1*gacE:3*ttgR:1*attL:1*gatL*ctcT*accE*ttgD:2*gatA:4*aacS*caaK*tcaG*tcgA*aacD:2*actL*attL*tttI*gctS*caaK:1*aagN*aaaR:1*atcL*gaaA*gctD:2*aacE*cagA:1*agaK*cagA*caaK*tctQ:1*gctV*ttcL+DNYANRSFD*aatD*ccaS*gaaD:1*gaaK*caaL*ttaK:1*aagA*gatE:2*gaaD:6*caaE:1*atgV:1*gatS+MEA:2*gtgK:1*ttaM*gccS*aaaL*gctN*cagH*aagN:1*aatA*gaaD*acaQ:1*attL:5*caaE:2*gttK*gatE:2*acgS:2*actN:1*ttgI*gttI:1*gctS*atcV:1*ctcE:1*ggcS*ctcA*aagE:1*ttaV:1*gacE*gccS:1*tatF:1*ttgF*gacE:10*tcgT:7*atcV:4*tccT:2*atgT:2*ggtA*gacT:3*gacN:9*cacY*gctS*gagG:2*cgtS*tctT*ctcA*aatT*gacI*ttcL*actS:1*tccV:1*gctP:3*ttaN:1*tctA:2*attL*gtgT:2*gttA*aaaR*tcaD:1*gcaT*gagV:2*caaN*ttcI:1*gctS*actY:2*gctL*actA:1*gagV*gaaA*tcgV:1*gaaD:2*aaaS*tctG*ttgV*cttQ:1*gatN*agtK:1+A*gagQ*ggcL*gaaS*gtgD:1*gagD:3*gtgK:1*atgI*gccN*ggaF:1*ttgI*gcaD:2*gagT*aaaH:2*gatS:1*accS:1*ttgV*actS:1*gacK:1*gccT:2*tctA*aatG*cttT*ttgH*aacL*agcN*aacG*gttS*gcgG*aacD:5*gaaD*actR:1*ttgM*cagA*aatR:2*gccE*tccA:1*tcaE*aagS:1*tcaN*tccA*cagK:1*actN*tcgE:3*aaaR*gatP*caaK:2*tctK*atcF:1*caaV*atcF:1*tttL*gagK:1*aacH*aaaE:2*gttL*tccA:6*aatT*tccA:1*gaaA*cttA:5*attS*gctD*tcaC:1*gagQ:4*aagQ*aacG:2*tcaA*attS:1*aagR:1*gaaA*tacF:19*cttA:2*aagN*attV:12*aaaH*tctT*catP:7*catN:3*gctS:2*gttA:10*acgS*tttY*ttgM:5*aatR:2*actG:3*aacA:16*actS*cagK*agaK*agcQ*aacQ-ggcggt*agtK*ggtE:10*aatP:1*gaaV*aacL:2*gtgT:13*gccS:1*atcN*gcaS:11*ggcS:1*agaV:1*gggD*gacE*agtD*agcE:1 +JAMAIE010000005.1:589225-655853 miniprot mRNA 38516 41650 2844 - . ID=BC000022;Rank=1;Identity=0.5523;Positive=0.7224;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +JAMAIE010000005.1:589225-655853 miniprot CDS 38516 41650 2844 - 0 Parent=BC000022;Rank=1;Identity=0.5523;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +##PAF 4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1053 0 1046 - JAMAIE010000005.1:589225-655853 66629 38515 41647 1623 3168 0 AS:i:2570 ms:i:2570 np:i:744 fs:i:0 st:i:0 da:i:0 do:i:6 cg:Z:99M4I201M1I7M1D7M4I265M1I39M5D134M1I39M2D33M1I153M2D57M cs:Z::2*aagS*acgN*atcR*tctA:1*tctV:2*cagN*actV*gccN*cttI*cgtK:3*tctN*gctT:2*actS:14*tggH:2*aagR:2*ctcK*tcgA:3*gctG*ttaI*aagR:1*ttgQ:1*attL:1*aatT:1*gaaD:1*cagM*ttaV:15*caaP:3*gcaR*gaaD*ggtA*tacQ*aggA:1*ttgV:1*tggF:1*gatQ*ggtS:1*gggA:1*tctG*ataN*caaN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aaaS:3*accR*tacF:1*atcM*agaQ:2*cagT:2*cggK*aatY:1*cgtP*ggtA:3*accM:4*gagD:4*gtgR:2*caaN*aacD:1*gatN:7*gatN:2*tccT:3*tcaG*cttV:2*ctgF:3*attV:1*gcaS*acaS*attL*tcaQ*catR*gatS*aagR*agaS*tctN:5*gcaS:3*cttM*gttI*actA:6*aagR:1*atcV*accI:5*attM*tacH*cgtV:3*tctN:1*gatE:3*ccaS:1*aagR:2*tacF*gagY:3*gctD:2*ttcR*gaaD:2*gctY:3*tccT*attL*cgcK:2*actR:1*ttgI*atcV:3*agaQ:1*ccaG*gagK*acaN*gctP:3*atgL*gttE*aagE*gatE*gaaD*tcaG*tccP*gtcG*gaaL*gagP*gagQ*actR:1*agaK*ccaS*gaaV*tctA*cgtE*cagT*acaR*aatS*ttgV:1*gaaP*ccaE*attP*tctA*gaaP*cagL:1*actV*gtcA*tccT*cagP*ttcT*cagP*caaS+I:1*attA*gctE:3*actI-ggt*gtcE*gatN:3*aatQ*cagD+ALLA:1*tctQ*agtQ*tttY*gagD:3*attE:2*caaR:1*gagQ*cgtQ*gatA*gcaM*caaE*gctE*ctcE*caaR*caaI*caaR:1*tatM*gctQ*gaaM:2*agaQ*attQ*ttgQ:2*cagM:2*gtcR:2*caaM:1*cagA:1*cgtQ*attR:5*cttM*cgcA*gagD:1*tacM*cagA*tcaR*caaH:4*gtaM:6*ctgI:7*gagD:4*ctaM:1*cagE:3*cagG:6*actA:1*attL*acaN*gctQ*accL*actQ:1*tcaT:1*actH:1*caaS*attA*ggcQ*tccA:2*gagD:2*caaE*aacS:1*catQ*gagQ:3*ctaM:1*aagR*aacQ:3*agtT:3*ctcR:2*caaS*ttgM:1*cagE:1*cacY:1*aacA:2*aatK:1*ttcL:1*aaaA*ctcT:7*caaK:3*gacE:1*cgtA:4*aagR:1*ctgM*aaaR*tcgH:2*gtgI:3*ggtD:2*cgtK:6*ttgY:2*gagD*aaaR*gttA:3*aaaN*ggtK*gatE*gagD*attV*gacE*aagR:2*ttgR*caaE*attL:1*gatM*ctcA*accQ*ttgD:2*gatA:1*gccK*gagD:1*aacS*caaT*tcaG*tcgA*aacD:2*actL*attL*tttL*gctS*caaK:1*aagN*aaaR:1*atcL*gaaS*gctE:3*cagA:1*agaK*cagM*caaK*tctQ*tcgR:1*ttcL*aatD*ccaE*gaaR*ttgG*gaaD*caaD*ttaS+D:1*aagL*gatR*aagR*gatL:2*ttaK:2*ctcE*caaY*caaE*acaA*atgL*gacN*gatE:1*ctgF*gtgN*gaaS:2*aaaL*gctH:1*aagE*gagQ*aatQ*gaaG*acaN*gctN*attS*gaaN*gagG*cagG*atcS*gacT*caaP-gttttggttgatcac:1*acgA*aagA:1*actH:1*ttgI*gttI:2*atcL:1*ctcE:2*ctcS*aagQ:1*ttaV:4*tatF:2*gacE:10*tcgT:6*attV:5*tccE:2*atgS*gacA:1*ggtA*gacT:3*gacN:1*atcL*attA:1*gggE*ccaT*aatD:2*cacY:4*cgtK*tctT*ctcV:1*gacI*ttcY*actS*tctT*tccA*attV*gctE*gacN:2*ttaS:4*attL*gtgT:2*gttA:1*tcaD:2*gagS:2*caaA*ttcL*ataV*gctN*actF:2*gctE*actS:2*gaaA*tcgT:1*gaaR*ttcN:1*aaaI*tctG:3*gatE*agtI*ttaI:1*ggcD+F*gaaP*gtgL*gaaD*gagD:1*actM*gatE*gtgR:1*atgI*gccT*ggaL:1*ttgM*gcaN:2*gagT*aaaA:2*gatH:2*caaK:1*actA:1*gacR*ttaM:2*agaK*tctT:1*cttI*ttgN*aacM:1-aacgtt*gcgG*aacD:6*actR:1*ttgM*cagA*aatK:2*gccD*tccA:1*tcaA*aagA:2*tccA*cagK:1*actG*tcgD:2*tcgK*aaaF*gatN:1+S:2*tctL*atcK:1*caaT*atcT:1*tttL*gagQ*attL*aacH*aaaE:1*attA*gttI*tccQ:2*attQ:2*accI:1*tccA:1*gaaA*cttA:2*aaaR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aaaR:1*tcaT*attS:1*aagR*actA*gaaQ*tacF:3*cacN:1*cgtK:13*cttA:2*aagN:4*actK:3*gttT:1*tctR*gggR*aatT*aaaS*tctG*catL:1*cagE:5*catN*gagN:5*gttA:12*ttgM:5*aatK:2*actE*gcgC:2*aacV:6*agtN:2*aatK:3*gacE:2*actN*cagK*agaK*agcF-aacggc:1*agtE*ggtL*gagD*aatE*gatK:3*tcaA*aaaA:1*acaS*aatK:2*aacF:2*gtgT:13*gccD:1*atcQ*gcaG:6*gaaQ*attM:2*tatV*ggcA:1*agaL*gacH*gggQ:1*agtE*agcE:1 +JAMAIE010000005.1:589225-655853 miniprot mRNA 38516 41647 2570 - . ID=BC000023;Rank=1;Identity=0.5123;Positive=0.7045;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +JAMAIE010000005.1:589225-655853 miniprot CDS 38516 41647 2570 - 0 Parent=BC000023;Rank=1;Identity=0.5123;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +##PAF 4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1054 0 1047 - JAMAIE010000005.1:589225-655853 66629 38515 41647 1620 3171 0 AS:i:2556 ms:i:2556 np:i:744 fs:i:0 st:i:0 da:i:0 do:i:6 cg:Z:99M4I201M1I7M1D7M4I265M1I39M5D134M1I39M2D18M1I15M1I153M2D57M cs:Z::2*aagS*acgN*atcR*tctA:1*tctV:2*cagN*actV*gccN*cttI*cgtK:3*tctN*gctT:2*actS:14*tggH:2*aagR:2*ctcK*tcgA:3*gctG*ttaI*aagR:1*ttgQ:1*attL:1*aatT:1*gaaD:1*cagM*ttaV:15*caaP:3*gcaR*gaaD*ggtA*tacQ*aggA:1*ttgV:1*tggF:1*gatQ*ggtS:1*gggA:1*tctG*ataN*caaN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aaaS:3*accR*tacF:1*atcM*agaQ:2*cagT:2*cggK*aatY:1*cgtP*ggtA:3*accM:4*gagD:4*gtgR:2*caaN*aacD:1*gatN:7*gatN:2*tccT:3*tcaG*cttV:2*ctgF:3*attV:1*gcaS*acaS*attL*tcaQ*catR*gatS*aagR*agaS*tctN:5*gcaS:3*cttM*gttI*actA:6*aagR:1*atcV*accI:5*attM*tacH*cgtV:3*tctN:1*gatE:3*ccaS:1*aagR:2*tacF*gagY:3*gctD:2*ttcR*gaaD:2*gctY:3*tccT*attL*cgcK:2*actR:1*ttgI*atcV:3*agaQ:1*ccaG*gagK*acaN*gctP:3*atgL*gttE*aagE*gatE*gaaD*tcaG*tccP*gtcG*gaaL*gagP*gagQ*actR:1*agaK*ccaS*gaaV*tctA*cgtE*cagT*acaR*aatS*ttgV:1*gaaP*ccaE*attP*tctA*gaaP*cagL:1*actV*gtcA*tccT*cagP*ttcT*cagP*caaS+I:1*attA*gctE:3*actI-ggt*gtcE*gatN:3*aatQ*cagD+ALLA:1*tctQ*agtQ*tttY*gagD:3*attE:2*caaR:1*gagQ*cgtQ*gatA*gcaM*caaE*gctE*ctcE*caaR*caaI*caaR:1*tatM*gctQ*gaaM:2*agaQ*attQ*ttgQ:2*cagM:2*gtcR:2*caaM:1*cagA:1*cgtQ*attR:5*cttM*cgcA*gagD:1*tacM*cagA*tcaR*caaH:4*gtaM:6*ctgI:7*gagD:4*ctaM:1*cagE:3*cagG:6*actA:1*attL*acaN*gctQ*accL*actQ:1*tcaT:1*actH:1*caaS*attA*ggcQ*tccA:2*gagD:2*caaE*aacS:1*catQ*gagQ:3*ctaM:1*aagR*aacQ:3*agtT:3*ctcR:2*caaS*ttgM:1*cagE:1*cacY:1*aacA:2*aatK:1*ttcL:1*aaaA*ctcT:7*caaK:3*gacE:1*cgtA:2*ttgM:1*aagR:1*ctgM*aaaR*tcgH:2*gtgI:3*ggtD:2*cgtK:6*ttgY:2*gagD*aaaR*gttA:3*aaaN*ggtK*gatE*gagD*attV*gacE*aagR:2*ttgR*caaE*attL:1*gatM*ctcA*accQ*ttgD:2*gatA:1*gccK*gagD:1*aacS*caaT*tcaG*tcgA*aacD:2*actL*attL*tttL*gctS*caaK:1*aagN*aaaR:1*atcL*gaaS*gctE:3*cagA:1*agaK*cagM*caaK*tctQ*tcgR:1*ttcL*aatD*ccaE*gaaR*ttgG*gaaD*caaD*ttaS+D:1*aagL*gatR*aagR*gatL:2*ttaK:2*ctcE*caaY*caaE*acaA*atgL*gacN*gatE:1*ctgF*gtgN*gaaS:2*aaaL*gctH:1*aagE*gagQ*aatQ*gaaG*acaN*gctN*attS*gaaN*gagG*cagG*atcS*gacT*caaP-gttttggttgatcac:1*acgA*aagA:1*actH:1*ttgI*gttI:2*atcL:1*ctcE:2*ctcS*aagQ:1*ttaV:4*tatF:2*gacE:10*tcgT:6*attV:5*tccE:2*atgS*gacA:1*ggtA*gacT:3*gacN:1*atcL*attA:1*gggE*ccaT*aatD:2*cacY:4*cgtK*tctT*ctcV:1*gacI*ttcY*actS*tctT*tccA*attV*gctE*gacN:2*ttaS:4*attL*gtgT:2*gttA:1*tcaD:2*gagS:2*caaA*ttcL*ataV*gctN*actF:2*gctE*actS:2*gaaA*tcgT:1*gaaR*ttcN:1*aaaI*tctG:3*gatE*agtI*ttaI:1*ggcD+F*gaaP*gtgL*gaaD*gagD:1*actM*gatE*gtgR:1*atgI*gccT*ggaL:1*ttgM*gcaN:2*gagT*aaaA:2*gatH:2*caaK:1*actA:1*gacR*ttaM:2*agaK*tctT:1*cttI*ttgN*aacM:1-aacgtt*gcgG*aacD:6*actR:1*ttgM*cagA*aatK:2*gccD*tccA:1+A*tcaA*aagA:2*tccA*cagK:1*actG*tcgD:2*tcgK*aaaF*gatN:1+S:2*tctL*atcK:1*caaT*atcT:1*tttL*gagQ*attL*aacH*aaaE:1*attA*gttI*tccQ:2*attQ:2*accI:1*tccA:1*gaaA*cttA:2*aaaR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aaaR:1*tcaT*attS:1*aagR*actA*gaaQ*tacF:3*cacN:1*cgtK:13*cttA:2*aagN:4*actK:3*gttT:1*tctR*gggR*aatT*aaaS*tctG*catL:1*cagE:5*catN*gagN:5*gttA:12*ttgM:5*aatK:2*actE*gcgC:2*aacV:6*agtN:2*aatK:3*gacE:2*actN*cagK*agaK*agcF-aacggc:1*agtE*ggtL*gagD*aatE*gatK:3*tcaA*aaaA:1*acaS*aatK:2*aacF:2*gtgT:13*gccD:1*atcQ*gcaG:6*gaaQ*attM:2*tatV*ggcA:1*agaL*gacH*gggQ:1*agtE*agcE:1 +JAMAIE010000005.1:589225-655853 miniprot mRNA 38516 41647 2556 - . ID=BC000024;Rank=1;Identity=0.5109;Positive=0.7039;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +JAMAIE010000005.1:589225-655853 miniprot CDS 38516 41647 2556 - 0 Parent=BC000024;Rank=1;Identity=0.5109;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 diff --git a/results/2026-10-30_barnettozyma/data/GCA_023627795.1_orf_scan.tsv b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_orf_scan.tsv new file mode 100644 index 00000000..15104143 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_023627795.1_orf_scan.tsv @@ -0,0 +1,9 @@ +seg strand wstart wend aa pfam best_ref +s68 + 30856 31194 113 MATALPHA2:18 +s203 + 25832 26341 170 Homeobox_KN:27:136-165,Homeodomain:53:112-167 460523_cbs-4732_MATog_MATa-MATalpha_MATa1:58 +s219 + 31193 31300 36 Homeobox_KN:19:13-35,Homeodomain:17:8-35 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha2:18 +s381 + 29172 29876 235 MATalpha_HMGbox:33:93-174 MATalpha1:62 +s593 - 24369 24887 173 MATA:33 +s618 - 16017 17015 333 dic1:362 +s699 - 38510 41662 1051 ANTH:260:14-275,I_LWEQ:4:848-872,I_LWEQ:195:891-1043 sla2:1217 +s739 - 24935 25288 118 460519_cbs-7435_MATkp_MATa_MATa2:16 diff --git a/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.a2.gff b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.a2.gff new file mode 100644 index 00000000..879555f8 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.a2.gff @@ -0,0 +1 @@ +##gff-version 3 diff --git a/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.fa b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.fa new file mode 100644 index 00000000..b76f42df --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.fa @@ -0,0 +1,833 @@ +>CM113344.1:1105885-1164023 Barnettozyma sp. AR-2024b strain PYCC 10016 chromosome 5, whole genome shotgun sequence +TAAGCACGTTAAATCAATCAAAGAACGTTGCTAAAAATGCTACAGTTCTTGTCAAGAACAGCAAGGTTGA +AGATCCTTTCTTGGCTAAAATGAGAAAGACCACTGCTATTTCAAGTGATCTTTTGAAGGCGATGAAGGAG +CGTAAAGATGTTGATCTTGAGAGTGAAATTAGATTGAAGTTGGGGACAGTTGCATCAATGGATGATGAAC +ATGAATACCATCGACAAATACAGGCCGTTAAGGATAAATATGGTGAGCATAGGTATCCTGCACCGACAGA +TCTGGAGATGGAAAATATTTTTTTGAAGACGAAATTGAAAGATAGAGTCCATGATTTGTTAACGAGTGGA +GAATTATTGGAAACCCCATCTTTTATCGAAGCAGAAAAAGTATGTGAAAAGTCAGGACACGCCACGATGT +CACATGGAGAGTTTCTAAGTCTTTCATTGATGGATTTATTCCCAGTTGGATATCTGGGAATGAAGAGACA +TTCTTCATTGGCCCATTTCATAAAGACGATGTTTGCAAAGGAAATCAGGGCATTGGGTGAGAACAAGGTG +TTGAGATTCTGGGGAGAAGATACATTTGTCTTGCACGTTATTGTCTCTGAAGTTGTTGTATGGATTGTCT +GTGATTATTTAAAAGAAAAGGAGGAGGAGGAGGGCATTATTAGGAGTACTACGAGTAGGGGAAAGAATTG +GGACCAAGAGGCAAGAAGAGTGATTAGAGAGACTGGTGATTATGGAAAGTTCATTAATGATGAAGTGAGT +TTGTATTGAACCTTACTTAGCCTTGGAAGTCCGCTTCTGTCACTCGCTCAGAATGTAATTAATGCGCAGA +TCTACAGACTACCAATAAATCATTTTTGTCTTCTTAGCTACTTATCACGAATTACGTTACGATAGATGAA +TTTCTTGGTAGACTTTAGTATTATCGTTAGATCAGGTAGAAAAGATTGGATAATTGTCACCAGAGGGTTT +GCATTGGCAAATTGACAACATCTCATATTGAGACACTACAAAGTTCGGTGGGGTGCAATTATAATTTACA +GACTAGTGACATTAACAACATTGTTCTGAGTTGTTTTGACTTCAAACAGTTTTCAAGAAAATCATTAAGA +TCAGCAGATGGATGGATCCCCATTGTCTATATAATAACTCCATAAATAGAGAGAGAAAGAGAGACAGTCC +TCAGACAAATAGCGGGTTTTCGAATGGAACTGTCGCTATTTTTTTTCGACAAGCAATCGAATAAAAAAAA +AATGTCTCAGACCTTTTTTTTTTTTGTTTCAGAAAGCCGAACGAACATCCGAAGTCCGAAGTTCGAACCC +TCCAAAGAACTTTGAACTTTGAACTTTGAACTTTGAACTCAGAGCTCTGAGAAAGTGGAATAGAAAAAAG +GGGAGTGGCTGCTGTTGATTAGTGATATATTCGCTTTGTTTTGTTTTGTTTTGTTTCTTACTTCAGACAA +ATTCCAGGAAAGACATGCTTCTCGACCTTGGGAAGGACATCAAGAACCTGTTGCTATCTGCGGAGTCCTC +GCCAACGGGCTCGCCATCTACCAGCAAGGGGTTCCGCTGGTTCAGAGGTAATAAAGGCACTGAGGAAGAG +GAAGGAGGGGAAGAGGAAGAGGATGACATCGTGGGAGACGATAAAGATGAAAACTCCCAGGATTCATTGG +GTGAAGATGCCGCGCGGGCCCTGATTGGTGGTGAGACCCTTGTGGAGAAGGAGGAGATTTCTGCCCCCGG +TGACACTACCACGACGGCTACGGATAAGCAGCAGCAGCAGGAGGTCACTACCGAGGACCTTAACAAGTCA +CACTTTATGAACCAACAAAAGATATTCACATTCAGTTTGCCCTTTGGAAGTAATCCATTGTCGATGCTTC +CGCTTCCCTCTTTGAGAAGCTTTACTCAGGGGATTCCGATCTTTGGTAGTATGTTTGAAGAAGAGGAAGA +AGAAGATTTTTTATACGATGGGTTGCATTCCAAGGATGAGATTGGGACTAAATTGATGAGACGAGAGACT +ACGTCTACTTTGGAGGAGGCATACTATTACCACGGCAGGAAAGGTAAAGGATCTTCGAGATTTAAGGCTG +TGGCGAACGCTTTAACTCCAAACATTACTCTTCCATCTTTTGATGACGATTCGGACAATGACAAAAGAAA +GCATTCCGTATGGGATACGATCGATGGAGATGTTGTTATTCTTGGCGGATATAGAGGTTCAATTTTACGT +GATACCAAAACGAAAAAACGGATATGGGTACCTATTCGTGCTGGTTTTAATATCAAAAAGATTGATTTGT +TTATCGGACCAACTGATGAAGATGAAGCTCAAACACAAAGGGAGATTTATTCAGATGATATCTTGTCTCA +TGTCGGACCTATCGATATTTCAAGAAAATTGGTATCTAAATTGGACAATGGGAAGACAACAGTACATACA +TTTGGGTATGATTGGAGATTATCTTCTGATATCTCATCTAAGAAACTTCTAGAGCTTCTACAACAGTTGG +AAAACAAGAGAGCAAAGAAGGGTATTGTTGGGAAGGGTACCATTGTGATTGCCCATTCGATGGGTGGACT +GGTTGCTCATCATGCGATGCAACAGGATCCATCATTGTTCAGAGGGTTAATATACGTTGGTGTTCCTGCA +GAATGTCCAAACATTTTGGGTCCCATTAGACTTGGTGACAGAGTCCTTGTTAATTCCAAGATTTTGACCG +CTGAAGTGAACTTCTTTATGAGATCGAGTTTTGTGTTTTTACCATTATCAGGAAGATGTTTTGTTAATAG +AAAGACACATGAACATTATAATTTGGATTACTTTGATGCTGATACTTGGGAGGAATACAACTTATCACCA +CCGGTGTCACATGACAGATTTCTTACAGAAGAACATCCTTTCATAACTCCAACAACAACAACAACAACTG +AAAAGTTTTCTACAACTTTTGAAGAAGCAAAGACATACCTTGAAAGAACGTTGAAACGTACCAAGAAATT +CATATTAGAATTGGAATACGATCAAGATAAAAAGTATCCACCATTGGCAATAGTTTATGGTAACAAAGTG +CCGACTGTCCGTGGTGTCCGTGTTGATTCTCCAGATGGTATTAAAAGAGGTGAGTATGATGATTTCTTTT +ATGGACCAGGAGATGGTGTCGTTAATCATAAATGGTTGATGCCCGAACGTAGAGGATTCCCAGTCAATGC +CAAAATTGCAAGTGAAAAGGGACATATACAACTGATGTGTGATTTGGATGCTATGGAGTTGGCCCTTGAG +GCTATACTATAAGATGGTTGGATTCTTGGTCAAAAGAAAAACAAAGAAGCAACAACAAACAAACAAACAA +ACAAACAAACGATGAATGTGATGAAATTATTAAGAAGAAGAGTCGTAGAAGTAGTACTTGACTTTGAGCC +AAAGGCTGCAATAGGAACACTGAAGGATTATTTCAAACCCTCCCATTCCATCTTCGGTACCGTTCTCTTC +AAGGTCGATTTCATTTAGAACGAATCCATCTTTAGAAGTGCAACGAGGACAATCCTTCTTGAAAACACAT +GGTTGTTCATCCTCGTTCTCGTCATCTTCTTCTTCCGGGGTTTCACATATGAAATCATCAAGAGTGAGGA +CATCAAGTCCTTCACCCGTGCTCAGCGTTCCATTTTTCTTAAAGTTCTCGATGAGTTCTGTATCGTACTC +TGCACGCAATTGCTCATTGGATAAGACACGGTATGCCTCCTTGATACGAGCGATGAGATCACCTTTGATG +GATTGGTTGGTTTTGTCTGGATGAGTAGAGAGGAGCTTCTCACGGTACGCAACTTTGATCTCAGTGGGCG +TTGCTGACTTGGAAATAGAAAGGACTGTGTAGTGTGATGTGTATGACATGCATGCGTTTGACAGAGGAGG +ATCTGTTTGACGTCGACGGTGATGATGATGATGATGATATTGATGATGACTCTCCAGAAAGAAGAAATAA +AAGATGTCCAAATTGTCCTAAAGCAGGAATTGCCTAAAGGGGCTACCATCATCCCCTCGGAACTTTCTCT +GTTTTTTTTTTTCTTTTTTCCCGGAACCAGAAATTTTGGAAAAATTGCAAAAAAAAAAGTTCTTCGAAGA +AAAGAAAAAAAAACAAGAAAACAACAAAAAAAACTTCCAACGAATTTAAAACGAACAAAATAACAGAAAA +GCTGTTTTAACGCACCGCAAAAACAACGTCAATTGAAACTGCTTTTCAACCGCAACAGTAGCTCATAAGC +TGTACAGTAAAGAGTAGAAAGAAGCACAGCAAACCAAGCCCCCCAAAAAAAAAGGAAAAAAAAAAAGATG +GATACCTTTGCCGCCGCCATCGAGGAGATCCGTGCCTCCAAGTCGGGTATTTCTGGTAAAAAGATCAAGC +TGCTGACCGACACCGCAAAACAACACGCCGGTCTTTACGCCCAGTTCGTCCCTCTCGTGTTTGAGTACGT +CAAGACCTCGCCTGCCACCCATAAACTCGGTGCCCTGTACGTCGTTGACTCCATCGCTCGTGGATTCCAC +GAGGATGTTAATTCCGGCAAAGACACTACTGGCGATTTACGCCGTGCTCTCGAGTTGTTTGGCCAGGGAC +TGAAAGAGGCCACTGAAATTGCACACGAATCAGCCTCTGACGAGCACAGAGGGAAGATTGTTAAACTATT +GGATATCTGGAGAAAGACTGGCGCCTTTAACGCGGGGCTTCTCGACACTATTAGACTCGGTTCAACGACT +CCAAACGCTCCTCCACCGCCATTGGCCAATGCTTCTGAAGCCAATGATCTGCTGAAGAAGCTGGCCTCTT +TAGGTGCTGCCTCAACCGGAGCCGCTTCTCTACCTCTACCACCGCCAGCACCACCTGCAGCAGATGCTGC +GGCAGCGCAAGCGCCAGCGCCAGCGCCAGTACCTGTTGCTGCTGCTCCTGCTGCTGCTGCTCCTCTTCCT +CAAGTTGCCCTACCAGCTGGGTTGCCAAACTTGGGTAACCTTGCGACTGCTGATCCAGCTCAACAACAGG +CTGCTCTCTTCCAAATGCTGCAACAATTGCAATCCACAGGCCAGATCCCACCAATGCCACAACCACCACA +ATTACCACAAGTAGTACCACAACAGGCCCAACAATTTCAGCAGCAATCTCAGGAGCAACAACCCCGTTGG +TCCCGTGGTCCACAGCCTGCATCACAACAGGATGCCTTTAATCAATACAGACAAGAAAGTGGTCAGCCAC +CATCATCATCATCATCAACATCAAAAAATTTCCGTGATCGTGAACGTCAACAACCAAATGGCTTTTCTCG +TCGTGATCGTGATCGTTCACCTGGTCGTGATCGTGCTCCACAACGTGATCGTCCACCTCCAGGTCATGTC +ATTGGTGAGTTGAACTTACCAGGTACGCCACATTATCGTGAACGTAACGTTTCTTTTGATCAATCCATGC +CTCATGGATCCATTAAAGTCTTGAGTCGTACTCTTTTCATTGGTGGTGTGCCTAATTACATGTCCCAGGA +GGAATTGGCTGCTATTTTGAGACCCTATGCTGAAGTTCAATCGGTTATTTTGAACCCAGAAAGAAAGCAT +GCCTTTGTTAAAGTATACTCTCGTAAAGAAGCTGAGACCGTTGTTGCACAATTCAACAAAACTGGCCAGT +ACACGTTGAGAACACGTTGGGGTGTTGGATTTGGTCCCCGTGATTGTTGTGATTATCAACACGGTATGTC +CATCATTCCTCTCGCTCGTCTTACTGAAGCCGACAAACGTTGGATTCAAGAGGCTCAATGGGGTGGATTG +GGTGCGGAGACACCATTGACACCAGGATACGTTATGGAAGAACCAGATATTGAGATTGGTGAAGGTGTTT +CCTCCAAGGCGATTTCTCAAAAGATGCCTACCGATGGATCTCGTAATGGTCCTAGATCTACAAAGCCAGG +TGAGCCAGATGATATTTATACTGATCCATTGGGCGGTGGTAATTTACCAAAGTTTATGCAGGGTGGGAAG +AGCGATGTATTGGCCAATTTGTTCAATTCTAATGGTGGTGGTGGTGCTCCACTTCCAGTTCCACAACAAC +AACAGCACCAACAGCAGCAGCAGCAGCAGTACCAACATCAGAATCAGAATCAGTCTCCACCACCTCCGCC +ACCTGGTCAGAGCAATGAACAGTTGAACAATCTGATGGCGTTCTTACAGCAACAGCAGAAGTAGTCACAG +AGAGAGAGAGAGAGAGAGAAAGAAAAAAATAAAATTAGTTTTGTATAATGTATCGATTTTATTCATGGAC +CTTTTTGACCTTAGATTTAATAACTTTTCCTTTTAGTATCGTAGTACTTATTCCTAGCAATGGGGTAAGT +CATTACCCGGAAAGTGAAAAATCATGAAAAAAATCCGAGAGACAAATCTCGAGAAAAGAAAGAAAGAAAG +AAAGAAACATTTGAGAGTCGAAACGGACAAGAAGAGACGCCATGTTCAGAGCTCCGGTCGTGTTGAGACG +GGGTCTAGCCACTGCCAGTGATGCATCCAAGCCGCTCAAAATTAAAGCTGCCATTCTTCTCACCCGTCAA +CCACTCCTTACACCAACTCAAACCGAGTTCGAGACCGCTTACTATAACTACCAGTCTTCTCTCGAGCGTC +GTTTGATGTGGACCTTCCCACAATACTACTACTTCAAAAAGGGTTCCCTTTCGGAGCGTCGTTTCACTAA +GGCACAGAAGGGTCCTGTTTCAAAGCAGCCAGGCGTCTTTTATGAGAAGGGTCTACCAGACGTGGTTCAC +AACAGAGAAAGAAGTGCGGCCCAAGAGGTGGTGATTCCTAAGGACGAATACGAAGAGGGTATGAGCAGTG +AAATTGCTAGACCAGTGGTGCCTAACTCGAGACTGACGGCGGCAGATGCCCAGGGCGACCAGAAGAGTCT +GGAACGTGCGCTCGATAGAAGTTTGTATCTGCTCATCAAGGGCCAGCAGGGATGGAGACTACCTTCCTTT +GACCTCGTGGAGGGAGATTCCGCGTTGCATCAACGTGCTGAAGCTGGATTGAGAGAGCTTGGTGGTGATA +AGGTGAACACATGGACTGTGGCCCGTAAGCCAACTGCTGTGCTGAAGGAGGGAGATGTGAGGGAGTTTGT +CATTAAGAGTCATATTTTGAGTGGTGAGGTGTTACCAAAGGACGAGTACGCCTGGGTTCATAGAGAGGAG +GTTGAGCAGTTGGTTGATGCCGACTACTTTGCAAAGACTGAGTTCTTGTTCTCGAGAGTGTAAATAGAAA +GAGTGAGAGAGAGAGTGAGAGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTACAATCATACTGTATAT +AACCAGGAATTGTCTGTTTGTAGTTCATTCTGTATGATTTTCTCAAAGAGTCGATCAAGAACCCACTATT +GAAGCCAGCTAGCAGTTCCTAACTGTCTGTTCACTTTTGATGATGATCTCATTTGTGTTATACGTAACAT +CGCGAAACTTCAACTCTACACACTTATAAGATACCTGAAGAGTTACTTAACTGAAGATTAAACCGTCGAC +ATAAACAGGAAAGCCCACTCTGATCTGAGCGCATTGACCATGTCATCGCTCTACAAACTCTCGATCTCCG +GTGTGCGCTCCTTCGAGCCAAAGAAGAAGGAGACTATTGAATTCGGCAGACCGCTGACGCTGATCGTTGG +AGCCAACGGTACGGGGAAAACCACCGTGATTGAGTGCTTGAAGTACGCCACATCTGGGCTCTTACCGCCG +AATGTGAACAAGGGTACGGCATTTGTTCATGACCCAAGCATTGTCGGTGACAAGTCTGTCTCTGCAGAGG +TTAAGCTGGCTTTCACATCGATTAATGGACAGAGCATGGTGGCTTCGAGATCAATGACGGTGACTGCCGG +CGCTAAGGGCAAATTAACCTTCAAAACTACCGAGGGCCAGTTGATGCTGATGAACCAGGGTGAGCGTACT +ACATTGAGCACGAAGTGTGCCGAATTGGATCTTCAGGTGCCGCAATTCCTCGGTGTTCCTCCTGCTATTC +TGGATAATGTCATCTTTTGCCATCAGGAGGAGAGTTTGTGGCCATTAAGTGAACCTGCTTCTCTCAAGAA +GAAGTTCGACGAGATCTTTCAAGCGTTACATTTCACCAAAGCTATCACTCAACTTAAAGACATCAAGAAA +GAGATTGCCGTCGAAGTAAAGATTTTGGAACAGTCCGTCAAACATTTGAAGATTGACAAGGAAAGGGCTG +ATCGTATGAAGGAGAAGTTGCAGAGTAGCGAGAAACTGATTGATGATTATACAGAACAGAACAAGCATCT +GAAGCTTGAACTGGATGAAATTGCGGAGGAAACAGACAAACTCTTCAAATCAAACCAAGCTTTTCAAGAA +ACGTTGTCTCACGTTGATAATCTTCGTCATCAGGAATCTGCATTGGAGATTCAACTCAATCGATTGGTTG +AAGAGACAAATATACTGGCAGACTCAGATTCGGAATTGAGTGACAAGTTCACCAATTTCCAAGCATACCT +GGATTCCAAGACAGACGCCCTAGACGCACAAAAGGCAGATCTTCAAAATAAACGAGAGGAACTGGCCCAG +TCTCGTTCATCTATTGATCAGCTTGTCCGTGAAGAAGCATCCTTGAAAGAATTAGAGAAACGCTACAATG +AACAGTTGGAACTCTTCACGCGAATCCAAGCTAAAGTCGCGACACAACTTCAACTACAAGCTGATTCACC +ACAGGAATTGGAAACTATCCTCTTCAATACACACCAGTCGTATCAAAACCAATTCAACCAGCTTCAATCC +ACATTAAAGGAAGCGGAGCAGATACTGACCAGTCAAATCCGTGCTCTGTCAGACAAGAAACTGAAAGAGG +AACAACATCTCCAATATACCTCTTCTGATCTTTCCACAACTCAATCATCAATTCAGCAAGTTCAAGCTAA +GTTGGCTCGTTTGTCAAACAATGAAGCAGAATTTACATACGCACAATCTACACTGGAATCACACACTTCA +AAACTTCAAACGTTGAAACATGAAGGGAAAGTTGAAGAGCTCAACGCCAAGATCAAAGAAGGTAACCTCA +ACATTGGAAGATTGGAGATTGAAATTGATGAAGTCGCAAAGGAGACTGCACGTTGGATGGCACAAGGTGA +TACCATGGCAAGACTGGGACTTTTGAAGGAGGAGATCAACTTCAAGAAGGTTTCACTTTCGAAACTAAAG +GAAGTCCATGCTGCCACAATTGATGAAGCCCAACTTGATAGTGCAAACTATATCGGTTCTTTAGAAGGTA +AGATTGAGGCAATTCAAGTGCAACTGAATGATAAGAGATCTGAGCAAGAACAACTCAAAACTAAGATCCT +AGAGGCTGAAACTGACCTTCGTCATGACAAGTCAGAACATGCCAGTCTCTCAAAAGCTCTGGATATCTCT +GCAAAAAGGTTCAAGAAACAACTTCCTGAAGATGAAGTGACTGATTATGACGAACAACTTGCTGAGGCCG +AAGAGGATTATTCCACTGCTCTTCAAAATCAAAAGATCTACTCAACCACCACGGAATTCAATCAACGTGC +TTTATCTCATGCAAAGAAGGATCATAGTTGTTATTTGTGTCGTCGATCATATGAAGCAGGCTCTACTATT +CTTGACACTTTTGTGAAAGAATTGGAGAAAAAGGTTAATGGTGGAATGGCTAAGGAGTTGGAGGAGCAAG +TTACTGAGGCAAAGGAGTGGGTTGAGTCTTTAAGGTCTCTAGGGAAGGATGTAGAGATTATTCGTCTGAA +TGGACCGAAAGTCACTGCTTTGTCCGGTAAATTGAAGGCATTTGAGGAGGATATTAAGCAGCAAAAGGTT +CAACTGGACCGAGTAATCAACTCAATCACTTCTATTGAATCTGATTTGATGAAACTAAGAACTTTAGAAG +GTCCTGTTCGAGAATTAACCCGTTTAATTCTGGAGATTACAAAGTCTGAATCACAGGTTCTGGAACGTGA +AGGTGAGTTTGCATCTTATGCTTCATCAACCAAATCTCTTGAAGAGCTTCAATCTCAACAAGCGGCGAAG +ACAACTGAACTTAAGAATCTACGTCATTTCATATCACAGTTTTCTGAGGAGCGTGATATAAAGATTAGAG +AGATGAGTGGATTAGAAGGACAAGTAAAGGATGTTAAGTTACAAATCAGTAATTTGGAGAATTCAATGTA +TGAAAAGATCAACTTACAAACACAGATGGAGGATTTAAAGAAAAAGGTTTCAACGTTTGAACAAAATGTT +AGCGATGCAAATGGTCTTATTAAGACTTTGGAGGATGAGATTGAGGCAAAATCATGGGAACTTCAACGGC +AAGTCCAGAACAATGAGGTCAAGATTCGAGAAGAGAATGAAAAAGTCAGTGAAGTCAAGGATCTCTATGG +TCAATATCGTGAAGTCATTTTACAGATTCGCAATTTCGAGACTAACACCAGACCAACCCTTGGTAGGGTT +AGTGAGAAGGTGTTGGAGCTCAAGAATGTGATTTCAGCTCGTGAAAATGAACTAGAGGCCATGAGAGAGG +TTATTGCCGTTGAGGAACGTAAATTGGGCTCAACTAGTGAAGAATTTAGAAACTTGAGGAACAACATTGA +CCTCCGTCAACTTCAGAACAATCTTGCCGATCTACAACTTGAGATTAAAAACATTGATATTGGTGTTGCA +GAGAAAGAGCGTGAAAATTATCAGGAACAATCTGCCTATTTGCGTGATCGTTATTCTGCTTTAAATGCAG +AATTGGCGGGTAAGACAGGTGAGATGAAACAAATTCAAGACCAAGTTAAACAAACTCGTCGTGAACTTAC +AGTTGAATTTAAAGATATTGACACTAACTACGTTACTGAGCTCACAAAATTCAAGTTCAAATCCCTGGCA +TTCAGTGATGCAGATACTTATGCAAAGGCAATTGATACTGCAATTATTAACTTCCATTCAATAAAGATGA +AAGAAACGAATAAGATCATTGAAGATCTATGGAAGGGAACGTACTCCGGTACGGATATTGACACGATCAA +GATCAGAAGTGATCCAGCATCATCGACTGGATCCAATAATAATAGATCATACAATTACCGTGTTGTGATG +GTGAAGAATGAAGTTGAGTTGGATATGCGTGGACGATGCTCTGCTGGTCAAAAGGTATTGGCATCCATCA +TTATCAGGTTGGCATTATCTGAATGTTTTGGTACCAATTGTGGAGTTATTGCTCTGGATGAACCAACTAC +GAACTTGGATGTTGAGAACATTCATTCATTGGCTAAATCATTAAGTACAATCATTGAGATGAGACAAACA +CAAAGGAATTTCCAGTTGATTGTGATTACTCATGATGAGAAGTTTTTGAATGCAATGGGGGCCGTTAGAT +ATACTGATCACTTTTATAGAATTCAAAGAAATGCGGCACAAAAGTCTCAAATCGAATGGGTTGGCATTAA +TAGAGTCTCTGAGTGAGTATATTAATTACATGTTGTTGAGAGGAAAGTTGTTGCTGTAAACAAGGTAGTT +AGAAGAAAGTATATTAATTAATCTAAAGATGAATCTATCCAAGAATTGTATAATCTCTGACGAAGAGCAA +TATCTTGCATTATTTTGTATTTTGTTTCAAACAATTTTTCTAATGATTTATTGCTTCTGGACTCGGTTTG +AGGAGGATACAGAATTTGACCTGGTTGTGTAAACTGTGCAATTATCTTAGTCACTGATTCTTGTTTATAT +GCAGCCATTCCAAGATATGCTGCACCTCTAGCTCCCGCAAGGTCTGGATTCAAAGTGCTTACTTCAACTG +GTAATTTCACAACATCTGCAATCAATTGTACCAGTCTGGTATTTTTGGCTTGACTTCCACATATACAAAT +TCTTTGAACTTGAATGCCTCCTTTTTGGAAAAGTTCAACCACTTGCTTCGTTTGGAAGGCTAGAAACTCC +AACGTCGCAACGTACTTGATGATTAGATCAGTGATTCCGGTATCTGTACTTTCTCCAAACCACACACCAC +GCATATGACTATCACCAAATGGTGTTCGATTCCCAGATAAGTCTCCATATAAGTATGCATGTTTAGCCAA +TAAATGAATGTTAGCACCATCTTGAAGCTCTATGATTTCAATCTCCTTCTCTAACCCATCAAATACGTTG +TTTGAATATTTTTGACATAGTTGAGTATATGCTGGATGCATTTCAAATAAATGCTCGATAAGTTTACCAG +TCGTCGATTGTCCTCCTTCAGAAACGAATTTACCTGGAACAATACCTTCATAAGGTCCCCAAATTCCAGG +AATGGTAACATCAGGGCTGTTATGTGCAAGGATAAAGCAAGTTGATGTACCCGCGACCATAGTCAATGTA +GAGTCCAATGTTGAGGTTGATCCCAAGAATCCTGCATAAGAGTCAATAATTCCCTGTGCGACAGTAATTC +CCAGTGAGTTTGAACCAACACGAGCACCGGCTTCGATCAGTGGATAGAATGATGATTCTAGAGCTGAGCT +TGATGGAAGAATGTGACCCGCCGGTGATGGTGGTGGTGGTGGAACAGCGCTGAAGTTGAAGTTGTCAGTG +GTCAGATGTTGAAGAAGACCTAATGTGTCAAGGAACTCACAACTCCAGCCCTGTAGTTCACAATCGATGG +CTCTTTGAGTCTTTCGATTGGCAAAACAATTTGGGGGGAATTCCAGTGGATTCCCACCCATTAGATGATG +GGTTAAGTAGTTATGGAGATCAATGAAGACCAGATCTGGAATGATGTGTTCTGGAGTATGGTCAATGATG +TACTTCAGCTTTGGAAGTGCCATCTCTGCAATCATGGACCCACCGTAGTACCTGAGCACATTATCATCAT +CATCAGCATCATATTTTTTGCCGTTGGTTGTTTGCAGAAGGGAGTTGATCCTTTTTGCTTCATTCTCAGC +ACGACTATCCATCCAGAACACAATGTTCTGAGTACTATCTGTGCCGTCTTTGGTGACACCATATGGTGTC +ACCACCACCAATTGTCCACAATTCTCTTTCACACGTCTGAAGACAGCCATTGAACAAGTTGCAGCAGCAG +CAATACTCACAATGGGTCTTCTCTCACTGCCAAACTTCTCCTTCACTTTCTCTAAACACACTTCTAAAGC +GTCAATGACACTTGTCGAGCTCTGAGTAACAAAGCGATTGTCGGCCGGATCAACAGTGTATCGCACGCAA +ACACTCTCAGTGTGCACCAACTGGCCCTCAGAGTCCAGCAGTCCAACTCTGACGGCACTGGAACCGACAT +CGATACCTGCGAACAGTGCCTTCGGGGCCATTCCCATGCTGGTTTTTAGAAGATGATGAAGAAGAAGAAG +AAGAAGAAGAGTCATCATTGACATAATCTCGACTTCTCCGATCTCCGATTCTCCTAAAAAAAAAAAAAAA +AAAAAATAAAAAAAAAATAAAAAAAAACATCGAAAACTTTTTTGACCAAAAAAACGATTTGTTTGTTTGT +TTGTTTGTTTTCCAACCAATTTCACAAAAACCTCTCCCCTCCCTTTTTTTTTTTTATGCGAAAGCTGAGA +GCAGCTGCAATGTCCACAGCAAAGCGTACACAACAACCACCATGGAAGACCCCCACCGCGTCGCCGTCTG +AATCCAAAAGCACCCCACTCGTTCTCTACAACTCATTAACCCGGTCCAAGGATGTATTCACTCCAAAACA +GGGCAAGAAGGTGACTTGGTACTCGTGTGGACCAACCGTTTACGATGCTTCTCACATGGGCCATGCTAGA +AACTACGTCTCTATTGACATCAATAGAAGAGTTCTTGAGAAGTACTTTGGTTATGATGTCCTTTTCGTGC +AGAACGTGACTGATGTCGATGACAAGATCATTGTCAGAGCCAGACAGGAGTATCTCTTTGACAACTGGAG +CAAGGAGTATAAGGGTCAAGAAAATGATGAGGAGAAGAAGAAGTACGTTGAGATCTGTCTCAAGAGCTTT +ATTGAGTCCAAACTGTCTGTTTCTCTAGAAGATTTTGCGACTTGGAAGGACAGTGTCAACGTCTCCGAAA +AGGCCGTCGAGGACCCAAAGTTCCCAATGCATTACAAGTCTGCAGTTGCAGCATACACTGCTCTCCAATC +CCCTACCGATGATGGTGAAGGTGAAGGTGAAGGGTTCCTATCTGCAGTAAAGGATGTTGTTGTGCCAGCT +CTAGACAAGGAATTGGGTTCCACTGTCAACAACCCGGAGATCTTCCGTTCGCTTCCATCCTTCTGGGAAG +CCAAATATGACCAGGATATGGCAAAGTTGAACGTTCTTCCTCCAACTATTGTCACTAGAGTCTCTGAATA +TATTCCAGAGATTATTGAATTTGTCCAAAAGATCATTGACAATGGCTATGCCTATGTTTCTAATGGATCT +GTTTATTTTGACACAGCAACCTTTGACAATGAACCACATCATCATTATGCTAAATTACAACCATGGAATA +AAAATCATACGGATTTGATTGCTGATGGTGAAGGTTCCCTATCGACAGGATATACCAAGAAAAACCCATC +TGATTTTGCCCTATGGAAGGGTTCCAAGCCAGGTGAACCATTATGGCCTTCTCCATTCGGTGATGGTCGT +CCAGGATGGCATATTGAATGTTCTGTAATGGCGTCCGATATCGTTGGTGAATCAATGGATATTCATTCTG +GAGGTATCGATCTTGCATTCCCTCATCATGACAATGAATTGGCTCAATCAGAAGCATGTTTTAACTGTGA +TCAATGGGTTAATTACTTTTTACACACTGGTCATTTGCATATTGAAGGTCAAAAGATGTCCAAGTCTCTA +AAGAATTTCATAACAATCGAAGAGGCTTTGAAATCATATACTCCTCGTCAATTACGTTTGGTTTTTGCTT +CGACTCAATGGAACAACCAATTAGATTTCAAAGAAGCTCTAATTAAGAAAACCAAGGCATTTGAATCCAC +ATTTTCAAAGTATTTTGCCACTGTTCGTGCTCTTCAAAACGATAAAAACTCTCGCCTTGCTTCTGGTGGT +GAAATCATTTCCAAGAAACTAGGTCCACAAGAAAAGGCCTTGTTTGCCTCACTTTCCCAAACGCAAGAAG +ACGTACATATTGCCTTCTCTAATAACTTATCCATTCCACAAGTGCTTGAGGCCTTGTCTGCCTTAACATC +AAAGGCTAATGCTTACATTGCCGCTATTGGCCCAGAAGTTAGAGTTGACTCATTGACACAAGTTACATAC +TATATCACTAACATCCTCGATATCCTTGGTTTCCCTGCCCGTCAAGACCGTCTTGGATGGTCCGATGATG +ATGATAAAACTGCTTCTTCTACCGGAATTTTTACATCATTATCATCATCATCTGATTCTCATTCTGATTC +TGTATCAGTCGAAGATGTTGTCCTGCCTTTTGTCAATGTTTTGAATGTTTTACGAGATAGCGTACGTTCT +GCTGCTATCGCTGATAAATTGGATGCCCAGGAGGTTCTCAAGGCAAGTGATTTGGCACGTGATGCCCTTT +TGGAGTTGTCTGTATCATTAGAAGATCGTTCAACTGATCAATCTGCATTGATAAAGTTCCTTTCTGTTGC +AGAACGTGATGAATTGTTGCAATTCCGACGTGAAAAGGAGGCGAGGGAATTGGCTAAAGCAGCAAAGAAG +TTGGAGCAGATGATATTGAAGGAACAACAAGAGAAGGAACAACGGGAGAAGGCCAAGATCCCACCTCAAG +ATCTGTTTAAAACTGTTGAATATAGTGAATGGGATTCTGAAGGTTTACCAACAAAGAATGCAAAGGGTGA +AGAGGTGAGCAAAAGTTTAACCAAGAAGCTCAAGAAGGTGTGGGAGAACCAGAAGAAAGTTCATGAGGCG +TTCTTGAAGACTTTGGAATAAACCTGTATTAATTATCGATCTATAACAACAAGTATGGTACATATATAGC +TTATAAGGAAAGCCATCAAAAACCGGAACTTGTAACTAATTCAACCTCGGAATTCCCCCGACAAAAGCTC +ATCGCCCACAAATTTTTTTTTTTCTTTTCTTTTCTTTTCTTTTATTTTCTTTTATTTTATTTTCCATTTC +GAAAGAGATTTTGAATTAAGGAAGCATTTGGAGTAGTAGAAGAAAGAGATATGGCTGGCAAGAGATCCAG +AGATGAGAGTGCTGAGCTCCGTGTCTCTAGTGTAACGACCTCCCCATCCATTGTCATCGGCTCCTTCTTC +AACGGGTTCGATGCTCCAAAGGATGCCAAAGTTGACGTTTATCAGACAAAGAGTGGTAAGGAGACCCTTT +TCCATGGTGAAAACAGCAGATTGGACTACAACGGTGTGATCTCGGATGATCGTGATCAATCAGAGTATGC +CATCGCCTTATATGATCCAGTGAACAAGTCTGTTGATCTATACCACGTCCCTCTTCTTTTGGGTTCTGTA +ACTTCAAAAGCCCATAGAAAACTGAAGGGTCCAGCCATCAAGACAACAGGTGTTCGTATGAACGTCATGA +GAAACACACTTGGTGAAGCTTTCGGTACCAAGAAGGCTAAGAAGGCCATTTCTGACTTGGAGAGAAACAA +AATCGATAGTGAACAACTTGTTACTGCACAGACGGATATTATTGATACAGTGAGATCCGGTACCACGAAT +CTTCCAACAAGAGAACAACTCACTGAAACTGTTCTTAATGACAGACCAACACCAATGTGTTATCCAGACG +CAACTAATGCTGCAGATATTTATCCTGTCGAGAGTATAATATCATCAAGGGAACTCAGTGCGGTCCGTGT +TGGTCCAATCATGAACGAGATGGAACTGTCGGCCAAACTCGAGTTGTTACCATACAAGGATTCACCATAC +ATCAAGAAGCGTATTGAACGCTATACAGACGACGACGACTCACACACGGAGAAGTTACAGCTTTTATACG +TTGTCTCTCTATACATGGGTCTTTACCACAACAGACGTATCTCCAACAAGGAGGCTCTTGCCACTCAGCT +CAATAACCCATCTGATATCTTGATTGATGCTCTTTTGAGCAAGTTTGCAATCTCTAAAGCAGGTCAGTTT +GGTCGTCATAAGGACAGAGCTTATAAGATTGATCCACATGGCGAGGATAGATTGCTTTGTTATTTGCTAG +CTGCCATTATGCATATTGACAGCTTTTCCGTCGAGATCTCTCCATTGGCACAAGAATTGTCTTTGAAGCC +TTCTAGATTGATTGGTTTGTTCAAGGCTCTAGGCTGTAACGTGAAACCAGTGTCCTCTGCTGAGGCTGAT +GCCTACGGAATCCCAAAGTCTGCTGCTGCTTCTTACAAGATTGCAACTTTGAAGGTTCCTTTCAAGATGC +CACAAATGACCAGAAGAAGAGGCAGAAAGACTTGATAGCTCAATCTGTTGTTATATAGATTAATAACCCC +CCCCCCTCCAGCCAATCCCAATAAAATTACGATTTAAAAAGGTAAAGGTAAAGGAAAAGGACTTGCTTGC +TTGTGTTTCTTAGTAGATTGTTGATGTTCATCACGTTTATCACGTGCTTCTTTCATGTCCCGATGATGAG +CTGAAACTGAAATTTTATCGAACAAGTACTTGGATAGATAGATAAATAAAATAGGGAAAATAACAAGCCC +AGAAAGATGACGGAACTGTAAGTCACTGTGTGTCACTGAACACTCCTTATGGAAGAGTGTAAATACTAAC +CAACCTCCCAGTAAATCCATTGAAGAAGCCCTCTTTGGTCTCGCGTCGGTCGAAGAAGAACTCGAACAGA +CCGAACGTGCTCTTGGTACGTCTTTCTTTCTTTCTTTCTTTCTTCAATGAAATGCTGGTACTAACTAACA +CACGCTCTTCATAGAGATCACCCGCTTGCAGACTCTACAGAAGATCTACGCCAAACGTCACAGCTATACC +TTAGCTCTCGACAAGTTCTGGCACATTGTTCTTGCTCAACACTCGGACTTTACAGAATACATCCGTGCCC +AGGACTTCAAATACATTGAACAAATCAGCGATATCAATGTCGAATGGGATATTAAAGATACAGATATGGA +TTCAAACCCGGCCAATTTCACAGTGACGTTCAAGTTTGACGCTTTGGATGAGGATTTCCCTGCACAGACT +GTTGTTAAGAAGTTCCAGCTGCTGGAGAAATTGGATCAAGAAGATAATAAAGAAACAGAAGGTAGTGATA +ATAACGATGATGATGATGATGATGATGATGAACCAGTGACTGAACGTCTTGTCAGCGAATCAGTAGATAT +CATCTGGCCAAAGAGTTATGACTCTATCAACCCTGCAAAGATAACCAACAAAAAAAAGAAGACACCAGAG +GGTAAGAGGAACTATAGAACCGGTATGAAATCCTTCTTTGGCTGGTTTAAATGGACTGGGAAGAAGCCAG +GCAAAGAGTTCCCATCCGGTGATGATTTGGCCAGACTGTTTGCCGATGATATCTTTCCTAATGCAGTGAG +GTATTATACTGAGGCTCAAAGAGATGGTCTTGACGAGGAAATTGATTCTGATGCCAGTGAAGCTTTAGAT +CTAAGCGATGATGGTGATAACAATAACAACGACGAAGATGAAGATGAAGATGAAGATGAAGATGAGCCAA +CTGAGAAAAAGAGACGAATTGCTTAAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGACAAATTCACAAC +CGATATATATATTAAAAGAAAAGAAAGTATTAAAAACATCATTACAGTATACCCACAAAGAACCCACCAA +AAAAACCACCCCATTCATTCTTCACCCTAACTTGATGGAACCGAGACCATTTATAATATCGTTCAATTTG +GCCTCATCAGCTCTTTTTTCAGCAGCTCTCGCAATTTCTTCACGCTTCAAGAAATCCAGATCCAATTCTC +TTCCTCCAGCAACAAGTTGAGCCTTTGGAGGGACAAAGATTGGTGTCTTCACTGAAACGGACTTGTTGGC +AATAGACTGTTTTTGTTGTTGTCTGTATTTAGCTGCAACAAAACGTTTCTCTCTCTTTCTTTCACAAGTA +TTAACAATGGTATCAGTGGCAAATCTGACAACCTTTTGAAGTTTCTGATTGTTACCAACTTTATTACCAG +TATGTTTCTTGTTTGGTAAAGTTCTTGGTTTTCCTTTACCACATGGTGGGAAGTATTTCATTTGGTCGAT +TGTCATTCTAAGTGGAATATAACCTGCGTGTGAAACCATTTTTTTTTTTGTTATGGTGATGTTGTTGTTG +ATGATGATGATGAGGTAGAGGTAGAAGGAAAGAAGGGATGAAAGTTTCGACAGGGTTCACTGTCTTTATT +TTATATATATACATACCTTTGCAAATTGGGGAGAATTTTGGGTTGGTTGGTTTTCTGTCTGCTTCAGAAA +TTCACCAAAAGGCTTTTTATTTAGTTAGTTAAGGAATGATGAGAACTTTCATGAGAGCCTCCCTCCAATT +AAATCTAGCCGTTTTTTTCTGAGTCTTTTACTTTTATCAACCCTTAGAGTCAAGCAAATATCCAATTAGA +CAGTATAAACTCCATGCTTGTGTGCTGTACTTTTGGAATTATATACAATATATATATATATATATATATA +TATAAACAAGCATTTTTTTTTTTTTTGCCACCAACTAAACATTAACTCCATCACCCCATATAGTATAGTC +TATGGAGTCAGCGAATCTCATAAAATGTACTGAACAGAAAAAGTATTAAACGAGTAATTTCCCAGAGATC +TTGGAGAATATTTCGTTATAGGAACTACACCCAAACTGTCGAAGATCACACACACACAAAATCTCTCTTG +ATTAACTCGTTATTTCTTTTTGCTTCTAAACCAGTGACTATAAACATGCTTTATTTGCTGCTCCTTATAA +CTAACTCCTCTACAACATCACTCACAAACGGTCATCCTCAGCCTCTGTCAAGTCACCATCACTTAATAAA +TCATGGTCATCATGCTGGGTAGTATGTAGAGATGTCTCATCATCCAAAATGTCATCAGAGTATGCCGTTG +CACTGGCAGAACTGTAGTCTAAAGCTACACTGTTTCTCTTCAACACTCTTGTCTGAACATACTCAACGAC +TGGAATCAAGATTGTACTCAAGAATCCAACGGTGACAACAACACCATATCCGACAATAGAAGATACCCTG +TTAAATGTCGTGTCATTATCAAACTCAATTGGCTCATTCTCCTCACCACCTAATCTGATCTGACCATAAC +GACCAACGTAATGGTTGTAGATGACATAGATCAAGACACCGGCACCAACGATAGGAATCAAGATGACGAG +AGCCAAAGACATCCCTCTTAGACCATGCTTTCTATTAAACTCATCTTCACGACCAGGACATGGACGAGCC +TCAACCTTGTCAAATTCTTGACCACCAACACAGGTTGATAACGGGATCTTTCTGTAACCGGTAGGCAACC +AATATTCATCACCACCAAACTCTCTGCATACAGAAGAAGTGTCTGGAGGAGTGTATCCAGGAACCAATTT +ACAATTACCAGCGGAATCTCTGTAATAATTGAAATCACATTCGAAATCTTGACGGGAACAAGCACAGTTT +CTCATCTCCTCGTATGGCTTGAGAAGTTTCTCACCAATATAACAGTCTGCACCAGGGACCTTTCTATAAT +ACTTGGCCTCGTGACCAAACAAACAATTATCAGCTTGATGTGGATGACGTGGGGTCCATAGATCAAAATC +ATCAGTATCAGGATGCAACAAGTCAAGCGAACACTTACGCTTCAACAACTGAGAAAAGTCAATCTGATAA +ACCGTGGCTCTATCACCAGAGGAAGCTGGTAAACGAGTGAACAACAAAAACTTTCTGGAATCATCGGTTG +GAACGGTTGAGATGTCAGCAACGACAACGAGGGAATCAGTGAACTTGTACTCTGTCCAGGTATCACCTTC +ATCCAAGGAATAGTAGATGACATCAGTGGAGTCCTGGCCGTCAACCAACACCAAGATAGAACCCTGGTCA +CCGTACTCCCATTGGTAGACACCCTTCTTGACTTCCTTCCATGTGACACCACCATCACGGGTCAAGTATG +TATTACCATCAAAGTATTGTCCCAATTGAGGACCAACGTTACCGACACCAACCATCATACCAATAGCAGA +GCCGGAGGAGAAAGTGTCTCTATAGTCAGCGCGTTCAGTGTAACCGTGTAAATGTAACGCACAATCCTTG +AGGGCCTTACCGAAACATTGGTTCTTTTTACCATCGGCATCAACAGCAGGTGGTTGCAAGAAATCCCAGT +CAGCACCGTCATTATGAGTGATTTTTGACTGTAATTTCTTTTTGGCACCTGTTTGTGCTTCTGATGGGTT +AGAAACAGTGTTTATTACTGCAACACCTTCCAAATTCTCCATCTGTTCAAAGTCAACAAACCCAGAACTA +TCTCTGTTAACAAACTGTTGGGACATGACATAAGAGGTTCCGTTAGAGTTTGACTTTAAGATGGCACCAA +ACTCAGTACCGGTTCTAGCATTGGTAGTAACATGTAAGAAAATAGCATGTGATTGAGCAGAAAGGACAGT +GTACGCCTGTTTCTTATCAACTCTAAAGTTGGATGGAAACTCAGCATTGGCCCAGTTTTTACCGTCAACG +GAGACGTGGGCTCTTAGAGATCCATCTTGAGCAGATGTTGCTGCCACGAGGAAGTCATCTGCTTGAACAA +ACCCAATAACGTTAGACAATGGGGTGAATGTAGTCTCAAAGTAATCTTCGGAGAAGATTAATTCTTCTTG +ATGAATAGTATGGTCATTCTCTTCTCTCGTACAAACGATCAAACGTTCATCACTTGGTTCAGCCAAATGA +CCAACGTAATCACAGGATTGGACGTGCTCTTGAAGCAATTGGAAACGTTTACCATATGATTGTGTTCTGT +AAGTAACTGTACGACAAGCGCCTGCATGGGAGGTATCACAATCTTTCTGCCCAATGTAGAGGAACCAATC +AGAATGACGAGCATGGAAACGAAGTGGGATGACACCAGGAATGAAGTTTGCTGGTGTTCTGAAAGATTTC +CAATTATCTGCTCTATCAGTGGAATAGATGATCTTTTGATTTGTTGCGAGGAGGTAAACATGATTAGTGT +TGTGTGGGTTGACAAAGATATCGAGGATTTCTTCCTCTGGAGCAATCTGTTCCCATGAAGATCCCTGATC +GTGGGTCACATAGCCTCTGTTCAACGATGTGATCAGGACAATGGTCTCATCCTTGAGATCAGAACCTGGC +ACACGCTCCAAGTAGACATACTTGAGGACCTTACCACTGAAACCAAAGGCAGATGTCTTAACCTTTCCGT +CATTCTTACCATCTTGAGACTCCTTCTTGTCACCTTCCCGTTCTTCTGGGTTTCCCTCATCTTTACCGGT +CTCATCTGGTCTGTCGTCACGGTCATAGTCGATTATCTTGCCTGCACGATGGTCGGAGGGGTCATCTGTG +ACAATCATGGCCTCTGTGTTTGTGGTTGTTGTGACAGGGTCAGGGACAGGGTCCTTCTCCTCTAAATCGG +CTGCAGGAGAGACCACAGGGACATTATTGATATCGTTATTGTTGTTGTCATTATTATTATTATTATTATT +ATTATCGTCAGCAACAGCAGCAGCAGCTCCGACAGCTCTGACCACGACAGCAGCGGTGAGCAGCACCTTC +AGTAATCTCATCTTTCGGTCGTGTAAAGAAAAGAGAAAGAAGAGGAGGAAGAAGAAGAAGAAGACGGAGT +CAAATCTGATTAAACCAACAAGAAGCTTTCCGAAGTCTCCGAAAATGCGGCGCGTACCGCTATTTCGGGT +GAACTTATCGGTGTTAACGCAGTGAGTTTTTTACTGAACCCTCTTTTTTTAACCAGTAGTGTGCCTTGTC +ACCCCACCAGATATATATATAGATAGCCTTTTGAGCACTTATTTGAGGGATAATGAATCGAAAATAATGT +AATGTAATGGGTGCCGGCTGTGATTGGTGGTGGTGGTGGTGGTGGGCACCTAATAGCTAATATCTACAGA +ATAACTACCAGTTTTAATCATATACTCGTTGCATTAAATAAAAATCAGTAAGTGAGGCCGTCTCGTTCTC +TGTAGTTGTCCTTGTTGTACTCCTGGCTTCTTCTTCTTCTTCTTCTTCTTCTCTCTCTTCTCTGATCAGT +TCTGTGCCCACGCGACCTTTTCTCCTTATCCTCCTCCTCCTCCTCTTCATCTCTGTGTCTATGGGACCTT +GATCTATGGCCATGATGTCTATGTGAGTCCGCTCCTGACCTTTCAGATCTCTCGCCATGATCTCTTCTGC +TCCCATGTGATCTACTATCCTCCTTATCTTTATCCCCCTTGATCCGTTTCTCCAACGCAGCTTCTCTCAT +CTTCTTCATCCTCTGCGCTTGGATCTTAGCCATAGGATCTTCACTCGCTCTGTTCATCTCTTCTCTTTGA +CTCAATATACGCATACCGCTCCCACTTTCATTAATACGATCAAACCCTCTAGTCTCCCTGACTGTTTCTT +CTTTTGATTTTTTTGCATTCAATATCAAGTCATCATCATCAACACGTTTCTTCCCCAACAGATAATCCTC +TGCAACGTCTGTCACAAGTCTACCACCTCCTTCACCAAGACCACCTGTTTCATACATCCAGTCCACTCTA +TCCGCTACTTTCTTCTTCCCCGTTTTGGCATATTGGAGCTCTCGAAGTTCAGTTAGCTCTAATTCTTTGG +CAATCTCCTCCTGGCGCTCTTTAATCTTCTTTCTTTCAGCAAGCGCCTCCTGCTCTCTCTCCCAGACCTT +TTGCTGGTTCTTCATGAGCCCTGGATGCCATGACTTCTTCAGATTCAAGTCTCCTGGTGCCATCTTGTGC +TGTGTTTGTTGTGTTTGTTCTGTTGTTCTGTTGTTATTTTACGTTGATTGATGTTTACAAAAAAACAAAA +AAAAAAAAAACTAAGCGATGAGCCTGGCTGGAACTTGAAAAAAAAATATGGCATCACATCATCATCATCA +TCATCATCACCACTTTGGTGGGACAATAAAACTACAAAATATAGTTATTAATAGTACCTGAGATTAAACC +TGAATAGAATGAAACCAAAAGCAAAACGGTAATAATAATAAACAAAATAAATGACAGTAGCACCAGCCTG +TTCGTTCCAACCAACCTCTTTGCTTATTTATTTATCAAAAAGGTGAGTGATATTAGGCCTCAAAATAATA +AGAGGAAGGGTAAAGGTAGAAACAACAACAACAACAACAACAACAACAACAGTAGCAAGTGGATTAAAAC +CCGTGGATTTTGATGTTCTTCTTTTGCAAAGCCAACTGGGTCGTCAAGAACTCCATCACCTTGACCCTTT +GGTCACCCTGCATTTGAATGACTTCACCCAACTCATCATCCTTGACGATGTTACCGTTGCAGGCAAAGTC +CTTCTTCAACACTTTCAAGATCTTCTTCAAGTCGTACTCATCTGGCAAACCTTGAACCGTGGTCAACGTC +TTACGACCGTTTCTCTGCTGGATACGGATGTGGATGTAGTTGGTTGGCTGAGTGTCATCATCGCCGGTGT +CGGCGAATGGATCAAAGGACTTGAGGTTCTGGATCGAAGAGGACATGTAATTATTGGTTGGTTAATTAAT +TAAAAATAAAATAAAATAAAATGTAAGCGCCTTCTTCTTCTTCTTCTTCTTCTTCTTTTTATTTTCTTTT +TATTTTCTTTCGTCAGTCGAAACGCTGAGAAAGAAAGAGGGTGTGTGTTACCCGGCTGTATATCACATGA +CCAACCGTGAATTTATTTAACTTTCTTTTATAAAAAATGACCCCCCGGTTCTAAGCCTCTTTTGGGCCGG +ATCTCGGAATTCATTTTGAACAGCGAGCCGATAAAAAAAAAACGGGAAATATCACAAATGTAAACATACT +AAACTAAAAGGACTAAAAGGACTAAAAGGACTACAAAATAACAGCTAAAAACACAAGGCGCTATGTCCAA +AACCATCTCTGAGTCCGACTTGCAGACCTCGGTTCGCAAGGCCACTTCAGCAGATGAGACTGCTCCGAAG +AGAAAGCACGTCAGAGCCTGTATTGTCTACACCTGGGACCATAAATCGTCCAAATCCTTCTGGAACGCTC +TTAAACTACTCCCAATTCAAAACGACGAGGTTCAGTTGTTCAAGGCCCTCATCACTGCTCATAAGGTCAT +TCAGGAGGGTCATGATTCCGCTTTGGCCGATGGATACAGAAACTTGAACTGGATTGATGGCTTGGGAAGA +TCTACCTTCCAAGGTGATGGCTTCAAGGGTTATGGCAGGTTGATTAGGGAATACGTCACTTACTTGATTA +GAAAGTTACTGTTCCATAGAAACCACAGAGGATTCAACGGTACATTTGAGTACGAGGAGTATGTCTCCTT +GGTCACGGTTCAAGACCCAGATGAAGGTTATGAAGCCATTTTGGAATTAATGTCACTCCAAGATTCTTTG +GATGATTTACAAAGATTGATCTTTGCCGCAATTTCTCATGACAAGAGATCTGAGTGTAAGATCTCTGCAC +TTGTGCCCCTCGTTGCAGAGTCTTATGGTATCTACAAGTTCATCACCTCCATGTTGAGAGCCGTTTATCG +TTCAACTGGTTCCGACGATGCCTTGGAACCTTTGAAGCAACGCTATGATTCTCAACACGCTCGTTTGTTT +GAGTTCTACGCTGACTGCTCCTCGATTCGTTACTTGACCTCATTGATCACCATCCCGAGATTGCCACAAT +CTGCTCCAAACTTGTTGATCAAGGATGATGATAATAACTCTCCTCCACAACCAGAGGACGAGGAGATCCA +AAGACCAGAATCCCGTATGACTAGTCAATCAACTAACCACATTGAGCCTGAGCCTATCTCAGAACAACTA +ACCATGTCTCAATTCCAACAGCCAATTGCTGTTCAACCAACAGGTGTTGATTTTTGGAACAATCAACAAA +CCGATTACGAAACTGAGCAAATTCGATTGCAACAAGAGCGTGAAGCTCAAACTCTACAACAACAACAGTA +TGCTGAACAACAGAGACTCTTGTTTGAGCAACAGCAAGTGCAACAGCAAGAGCAACAACGTCTCGCTCAA +GAGAATCTCATTCGTGATCAGTACCAATCGCAAGCTCAGGGTCGTGTCGCTGAATTGGAACGTGACCTAT +TGGCCTTGAGAGGTCAATATGAACAAGACCAATTGTTGCTACAGCAATATTACCAGCGTATTAAGGCATT +GGAAACTGAGATTGCTACCACTGCTCAATCTGCCACTCAACAATTGGGATCTAAGGATGAATTGATTCAA +AATCTACATGAACAGATCAATTTGTGGAAGAATAAGTATGAGAGTTTGGCTAAATTATACTCTCAATTAA +GACAGGAACATTTGAATCTATTGAACAAGTTTAAGAAATTGCAACAAAAGGCTGCTTCTGCTCAAGAAGC +TATTGATAAACGTGAAAAATTGGAAAAAGATCTCAAATCAAAGAATGTCGAATTGGCAGGATTGATTCGT +GAACGTGATCGTGCCCGTTTGGACTTGGAAAAGTTGAAGGGTGGTAAAGGTGATGAAATTGATATGTTAG +AATTACAAATTAGAGACTTGACTTTGAAATTGGATGATTCTGAAAGAAACCAATCATCCAATCTTTCCAC +TATCTTTGCTCAGCATAAGAAGGAAATTACTAATTTGGAATCACAATTAAGACAACAACAATCTGCTTCT +TTTAACCCAGACCTTGAACAACTACTTAAGGATAAGGAAGAGGAATTGGAGATTGTTCAACAAACAATGG +ATGATGCTCTAGCTGAACTAGCCAAGGCTCAACAGGAGAATGATTCCGCCATTGAGGAACAGATTGATCA +AGTCCTAGTTGATCATTTAACCAAATTAACCTCGTTGGTTGATGCTATCTTACTAAGTGGCCTTAAGAGA +CTTCAAGATTCCATTTATGAATTGGATTCTCCAATGCAAGCAGGAAATCAAAACTCTTCTCCAGAATTCT +TACTTTCCATCATTGAAAAGTCATCTACATCTGCAACTGACTTTGCAAACTCTTTCAACGATTTTATCGC +TGATGGACCAAATGGTGATCATTCTGAAATCATTCGTTCGTTGAACGATTTCACTTCTTCTATTTCAGAT +GTGTTGTTGAACTCCAAAGGTATTGTTCGTTTGGTTAAGACAGATTCCGAGGCTGATCAGATCATTTCCT +GTGCTAGATCCACTGCTGAAGAATCTGAAGAGTTTTTTAAATCATTACTTTCCGATATGTTGGAAGGTGA +TGAAGAAGCAAAAATGGAAGTTGTTATTGTTGGTAATTTGAGTGTTCAAGAAAAACTTCAAGAATTGACC +CAACTGGCTGAAGATTTGGCTCCAAAATCCAACCTTTTGAACAATAACAGAGGTGCTGCTAACTTGAGTG +ACATTGTTGATAATGAATTACAAAATGCAGCTCAATCTATCGCTACGGCTTCTTTGCAATTGAATTCTTT +ACTCACCAAAAAGCAAGACCCATCAATCTCTCAAATTGACTTTGAAATTAACAAGGCAATCTTAGCTGCT +GCGATTGCAGTTACAAATTCAATTGAGATTTTAATCAAGGCTGCAATTGCTTCACAAGAGGAAATTGTTG +CTAAGAACAAGGGTTCTATTTCAAAAACTGAGTATTATAAGAAACATAATCGTTGGACCGAAGGATTAAT +CTCTGCTGCTAAATCTGTTGCTCTTTCCACAAAAGTTTTAATTGAAACTGCAGATGGTGTTTTATCAGGT +ACAAAAACACATGAAGAATTAATCGTTGCTTCCAATGAAGTTGCTGCATCCACTGTTCAATTGGTTGCAG +CTTCAAGAGTTAAAGCAACCTTTTTATCAAAGACTCAAGATAACTTGGAAGCTGCATCTAAAAGTGTTAC +TTCTGCATGTAAAGGCTTGGTTAATCAAGTACAGGATATCTTGACTAGAAGAAGTAATGGTGGTGGTGAA +AATGATTTAGATTATTCAAAGTTATCTAATCATGAAAATAAGACTGTTGAAATGGAACAACAAGTTGAAA +TTTTAAAGTTGGAAAATGCATTGATTGCAGCAAGAAAGAGATTAGGGGAGATCAGAAAGTATGGATATAG +AGATGGTGACAGTGACGAAGGTTGAAGACGGAGCTTTGGAAGAATTAACTACTAATTGTTTGGTCATTTT +CTTATCATCAGTGAAACAATTGAAGGATATCAACTAGCGGCCGCTACTCCCTTTGAACGCATTCGTTTGT +TTGTAAACCAAACTCGTACCTGTAAAGGCGTCATGCCACATTTTTCAGCAATCGCCGCTCGTTCTTTTCG +ATTTGGTGTCTTAATCATTTCAAAGACTTCCTCCAATAATGCCCTTGTTGCCTTAGACATTGGGGTTCTC +TTTTTCTTCGTTTCTGAAATGTTCACGTTTTCGGTAGTGCTTGGTGATCCACTGGAGTTTTCCTCTTCGT +TAAATGTTCTGGGTGGAGCCTTTGTAGAAAGTATGGATATAGACATGGTGATAGCGATTAACCCAGTAAA +GATCCTATCTCGAGAGGATGGTATGCAGCGCACATCACCGTTAATCTATTCAATGAAGCCACACAGTAGC +TTAAGGAAATGTATCAAAATATTGGTGTACGTCTGGGACCTTTGATGCATCTACAATAACGCTCAACTTG +AATCTAAGTCTCTCCAGGGACGATTAGAGTCTCAAGAAACAAGTAGAAATATCTAGGAAAAACATTATAT +TTGTGTGGTAACGCCCTATGCAGCGTGATATCTTCCAGGATCAGGGCGATATATTATTGATAAGAAGTAA +ATTTAGGTTCAGTTTCTACAGAAATTCGCTTGATGGGCTTATAATATATCAGGTCTTTACGCCAGTTTGA +TAAACTTTGCTTCATTAAGTGTTTTCCTTTAAATTATGAATATTCGATATGGGTTAAGAGGTGGCACCCT +GTGATATGTGACGTCGACCATAATCTTTTCAAATGAGACAAATTTTACCGACTGACACGTTATATAAGTC +ATAATAGTCAATATTCAATGAACACTTATACGAGTTTGCGCTTTTTCGTTCGGTCAAGCACTTTGTCACG +AACATCAACCTTGTATTGGCTTGAGCTCCTGCCCTCCCCTGGGTTAAAAATATCCTGAATTCGTGAGGGA +TCTGGTGTAGTATGAAGCGATCTATTCACAGCTTCCTGTAATGCCTCTGATGTCGAAAGTAAAATCCAGT +CAACAAACGTCATTTCGGGGTGCTGGGACAAGAATAAGTTGTACTCAGCAGCATAGCGAGCCCAAATCAA +CTGAAATCCAGGACTACTCTCCCATTGCTTTGCTAATATTAATGAGAGGTCGCCTTGGTGCTCTGTGGAA +ATGTTCCTAGAAAAAAAAGATCTGTATGCAATGAATCCGTTGAGTTTCGTGCGTTTTTGTTTGGGCTTGT +TTAATTTCTTCGGCACTGTGTGCATTTGTTTTGAATTCTTCTTCTTCTTCTTCTTATTCAGCAGGCTTGC +AATAAAGTTCTGGCATTTTGTTGAAAGCTTGGGTATTTGCGGCAGTGAGGACGCAGCAAAGGTGCCCTGG +AACGATGCAGGGTCACAACGCCTCCGTCTCTTTTTGGGCCCCTTGGAAATAGTTTTCAAAGGTAACACAG +CTACTTTAGGGAGATCGGGCCTTGAAGTCCTTTCGATCTTATCCCCTGGTGTCTGCACGCTCATTGTTTG +GGCATCACAAGTACTAGTGACAGTATGTTGGATGCAATTTGCTTCCATGCCCTTCTGAGAGTTCTCCGCG +ACTATTTTAAAAGACTTTGCCTCAAAAAGTTACAGGACCCTTATTTTTGCTGAGTAGTTGTCCGCATGAG +TTCGTCTAATTTGATTTACATTGTGCAGTATGTCATCAGATCATTCATTCCGGCTTTAATCCCTGATATT +TTAGTATGGTTACGTAGCTTCTCCTTAGAGTTGGAGCTGTTTGTCAATCATACAAATCAAAGCAAGTACT +TAACTAGTGACCACCTATAGAAAATCCCATCCGCCTCACAAAATCGAGAATTACCAGAACATATTTTAGC +TGGACCCTTCCAAAAAAATGAAGTTTGAAGATCAATTTACACTAGATAGCTTCTACGGGTGATCAAAGGA +TCTATCGTAAAATTCAGAAATACCGTCTCAGTAGTATTCGTATTGCTTCAAATAGACAGGCGAACCATAC +TCACAGATTTTTGAAGAAGTTTCCCACATTTGCGTGAATACGGAAAAAACACTCACATGAGTCACGGTTC +ATGATTCACGACAGTGTTTATGTACAAAACTTGTAAATTGGCGGGTAGGTCCTACAAAGGCTTCATAGAG +ATTATTAATAAGAAAAAGACACAAGAAAAAGATTCACAGTCGGAGTCCATACTTAATCACCTCCTTTCAT +ACGCTTACGAATTCGAAGCAAAGCGTCAGCCATAATTTCATCGATGAGTTTCTTCGCTTCTTTGCTCAGT +TCTGGTTCTTGCGATCCAGAAGCATTCTTCTTGACGGCTTTCATCTTACTTCCACCACAAGACTCAACCG +TTGAGTAATCACCAGATGTAGGTTGTTGAGAATCAGTACTCTTGCCCTCAAGAGAAGAGTGAGAATTGGA +CTTAGGCCTGGAAGACCCAGCCTTGGCGGACTCAGCCTTGGAAGCAGTAGACGTGGCAGAAGCTGATCGA +TCACCACCAACAACCTTAGCCTTGGTAGGAGGCTGAGCCTTGACAAAACGAGCCTTGGAAGCAGTAGATT +TAATACCGGTAGATGGTTTAGGCCGAGCCTTGTTGTATTCGGCAGAACTAGAGGAGGCTCCATCCGAGGA +CTCGTCAACAGCAGTAGAATTGGTCGCGGTGCGCTTGTTTTTCCGGGCCACCTCCACAGAGTTAGATTGC +AACACCTCTTCAAGAGTAGGAGGAGTTGAGGAAGTGCCGTCTGAAAAGCGAGGCCGATACTTTTCAAGTC +CTCGCATATGTGAGATTAGCTGAATATCCAAACACCTAGCGGCTGACTCAACCTCATTTTCTTCTCTGAA +GTTCTGCTCTGTATGCAAGAGGACTTTGTCCACGTAGGCGATAGGATCATCCTTCCATGTCACCATTTGC +TTCATCCATTCGTCATATTCCTTTTTCTCAACAAATTCGTGGGTAATGCACTTGCTTGTATCAGGCATTA +TAACGATTTTGGTGGTAATTGAACGGCCTCCATAGTCTGAACTCAGGTGGTCATCTAAGGCAATACCTTG +CAAAATGCTATCCGCCAAGTCTTCAGTGACTTTGAATACGTCCGGATTGTACATAATCAAGTCTGAATTG +AGCTGTTCGTCAGTTTTCTGGATGCCAGTAAATCTCAAAACTCGTTCGCGGTAAGCGGGATCATCAATCA +TCCGCTGCCTTTGAGTCTGGACCAAGAGAACACATTCTCTATAATGGAATAGTACAGATCTAAGGACCTT +CAACCTGGAAATTCGATCGATCCCTTCCAGTGGTTCAGGGCTATAGCTTTGCATAATCTCATCCAAAATT +TCAGTGCATATCCGTGGATCTGGCCTGTCCTGACAGATTACCATCTGGCTATCACCTGAAGGTTCAATTC +TTCCCGTATGGTAGTTCTCAATATCGTAAATGTCGCCCTTCTGCTTGTATTCATGTGGCCTGATCTTGAA +AAACACAAAGAGTTCCCCAGCTGAACGGAACTGAGCCAAAGCATCCAGTGTCATTGGACTTGGACAGCAG +AGCATTGTGACGACAGGCATGTCAGTTATTTCTGAGTGGCGTCTTGATATTCCAGAAAGTTGTCTGATTT +CAGCTGGTGTGATCTTGCGCGTTGGTGTGTTCTTGCATTGGACAATCACCTTCACCTCTTGATCACCTGT +CCCATGGAAGATTGGTGCCATTATATCTGCCTGTAAAATCTCAGCGACCTCTGTATTGGGTTGTGGTTGG +TCCGGCTGCTGAAGTCTCATAGTTCCCTTCAAATCGGCGCCCAGGTCCCCTTTCCCTCCAGAACGCACCA +AATGGATGTTCAGGGCCCTCATCAAAATTTCGCCAGTTGATAGTTCGTAAAATATGCCCTTGTTAGTCGA +ATTGGACCATGGCGTCTGTTTTTTTGATTTCAAGTGTTGAAGAAACTCTCCCAGTGTGTTATAAGAACTT +GATTTGGATCTCACAAAGAAGTGCACCGTTCTCTGAAGCTGTAAGGTTGGCCTATATAGCTGAGATATGA +TCATTGTCCGTGATGAATGACTTGACTATACGCAAGTCTTGAGGAGAAAAATGCCTCAATTTATATTGCA +TTTTAATTCGTTTCATGAGATGTTGGTGTCGCTTAATGAAGCAGTGTCAGTTTTGTTTCATCTTGAGTTT +TGTTTTTGTTTCGAATTGGTTTGGCAGTTTCACTCGCTTTTTTTTCTGAGGAAAGTTGTGAACTTTCCGC +GAATCTATCTCCCTCAAAATATAAAAGATCAGAAATGGTTTCAGACTTCACTACCTCGTAGTACCATTTT +TTCAGACTATAATGAACCAAGAGCACACCACTAGGTGTCCAATCAGCTCGCTTTTATCTGATACTGGCAG +TAATCAGGACATGATAGGAAGGTGCGTTATCGTGAATCTTGGATTTGGATAGCCTGTACTAACTATTAGA +ATTGAAAGATCAGATCATTTCCTCGAATTGAGATCAAGCCTTGTACTGGAGAACTCCTCACTTTTGGCAT +CTTGTGATTTCGATACGTTGTGCTCCAACATTCTGGCATCACAAATGGTGAGTAGCCGTCGCAATATAGA +TCTGGGATTAGCGACTAACAAATTCAGGAAGTTCAATTCTTAGATGCTAAAATTGCAAGCAGAACCCAAA +GAAAGCGCTTGGGACTAAGTGCCGCCAAAAGCCGATTCAGGCTATCGAGTATTCTACTAAATGAGAAGTC +GATTTTGTTAGACGCAATGGATAGATATTCCAGTGCGACCGACGAATCACAGGGAAACCAGCCGTTTAAA +TTTAGCATTATAACGCAAAGGATGATCAGAGAAGACGGAAGTGTTCTTTTCAGAAAAAAGAGACTAGGAA +AACATAGGGTAGATATGCTTGAGGATTGGTAAGTAAGCCGCAAACTTGTCTCACTTTTCTGATTCTAACA +AAGTCAGGTTCGCTAGTCATCCCGAAAGGCAATTGTTGAACAAATCAGTTATTTTACAGCTGGTCAAGAA +AACCGGCCTCAGTAAAGGCCAGATTCGAAACTGGTAAGTCAAGAATCCATTCTATTTTTGAAACGATGCT +AACATTGTTAAGGATTTCAAACAAGAGGTAAGTCAATTCGATAGTTTGTCGTAACCGCACTAACAATCAC +CGACAGAAGAAAGGAAAGAGGGGGTAACGTTGGTGATATCATATCCAACTTGGTGTCTCCAAAAACCCAA +ACCAATCATACCTCTTAGTTCTAGCGATACTCTTAAATGTAAGGAGAGACTTATAATCAATGATCCCAAA +CAATAATTAAAGTAACATTCTGATTTATCAAGAAGTCGTTTAGTTCGTTCAAACCAACCTTTTCTTCATG +CGTGAAGGGTGGAGACACCGGTGAGATTTTTTTTTCTCAAAATCATTATTTTATTTAATATATTTCGATT +TATGCCCTTATAACTCTGCTCAATCCTCAGCCTAATTCCTTTGGGCGCGCTTCTCTTTTTATTATTTCTT +GTCTGATTCTTGGATCATATCAATTGTCAAAGTTGTAGAGAGATTTTTGCTGGTTCCGTCAGGGTAATAC +TTTCAACCCTAACTCTAAAAATCCCGAATTCCTCCGGGTCCCTCCCCCCCCTCCCGCAAAACACTCTAAC +TCTCTGTCTCTTTCTATTTCTCTTTGTCTTTCAACGGAAAAATTCGGTTAAAGAAAAGTCCGGAAAAAAA +AAATATGAAAAAAAGATGTATAGCCGAATCTTCCTTTTTTGATCCCATTTACTAAGATAAGCAAGCATCA +GATCACACACCAATAGAACACCATCGCTTCAAAAGCCCCTCCCCCCACCCCCCCCCACCCCCAAAAAAAA +AGATGTCTTCCACCTCCCAACCTACCACCCAGCTGTCCTTGCAGTCCCAGATGGCCACAACCACAACCAC +AACTCCTGTCACAGCCGCATCAAAACCAACTCAAAAATGGCCCTTTTGGTACGGCGGGTTTGGTGGTGCC +GTTGCTTGTTGCTTCACTCATCCTTTGGATCTCGCCAAAGTACGTCTCCAAACCGCTCCTCTTCCCAAAC +CATCCCTTTTCCAGATGGCTCTTTCCATTCTTAAACAAGATGGTATTCCCGGTATGTATTCTGGATTAAG +TGCTGGTTTATTGAGACAAGGTACTTATTCACTCACAAGATTCGGAGTCTACGAGTATTTGAAGGGGAAT +TTCGTCCCTGCAGACAAGACAAAGTCAATGGCTTATCTTCTACCTATCTCCATGTTTAGTGGGGCCGTTG +GTGGGATCGTGGGGAATCCATCCGACATTGTCAACATCAGAATGCAGAATGATACGGGTCTTCCTCTTGA +TAAGAGAAGAAATTATGCGCATGCTTTTGATGGTGTTATTAGAATCGTTAAGGATGAAGGTATGAATGCA +CTTTTCAGAGGTCTATCTACTAATTTGGTCAGAGGTGTTCTTATGACATCTTCTCAAGTTGTCACGTACG +ATTTGGCAAAGGAACTTCTAATCACTTTAGGAATGAAAGGAGATTCTTTCTTCACTTTTTTTAACTCTTC +CCTGCTTGCAGGTTTGGTGGCTACCACTATCTGTTCACCAGCCGATGTCCTCAAGACAAGAATCATGAAT +GCATCATCAGCAGGAAATCAATCCGTCATTGAAATCCTAACAACTGCAATCAAGAATGAGGGTCCAAGTT +TCATGTTTAGAGGTTGGGTTCCAAGTTTTGTAAGATTGGGACCAAACACTATCATTACCTTTTTGGCAGT +GGAACAACTCAAGCATTTAAAGGTGGGTCTTTGAAAAAAAGATAAATTTTGGCTTTTACCATTCATTTTA +TTATTCATTTTTTTTTTTTGTGTGTGGTATGGAGATTTTTTTATAGTCAAAAAATAAGTTAAGTTTCATT +GGCAATTAATACATACAAACCAATAGTTCCTTTTATTTATTTATTTATTCAATTTGGGGCGGTAGTCTGT +AGTTTTATAGTACCCTGTTGTTGAAAATAAGCTTCTTCTGCTTTTAAGATGGAACTCCCATTTTTTGGTT +TGAGTTTGAATCGATATTCTATAAAATTGGGGTCTCTTCAAAAGGAAAACTAACTAGCTAAGTAATCAAT +CTATCTTTCTATATCTGTACACCCATTTTCTTCAAAACATCATCAGTTGTCTCTTTTCTCCAATCAACAG +CGTCTAAAATCTCTCTAATTTCTTCAGTTCCAGTAGCAAACATAACACTGACATTAGAGTCCTTATTTTT +AACAACAACAACATCATCATCATACAAACCCTTATCAGTTTCTTTGCCCCCCTCAATATCAATCATTACA +ATGTTAACTTCACTACTACTACTTCTTCTTTCAGGTGACATTGACGATAGAGAAGAAGAAGAAGAAGAGG +ATGAAATCATTTCTTCATTGACGTAATGATGATATGTCGTTGTGCTGTCATCATCATCCTCCTCCTCTTC +GTTCTTGATTGGTGGTTTGAATGATGGTGTCATCAGCACCGTGGGGTCTATTTGTTTCAAATCATTCAAT +AGTAAGAAAATGGAATGTAGTTTCTCAGTGCAGAATTTGTCACCATGTGTTGTCAATTCAGTGAATGATG +ATAATGAGATGGGCTGGAACCGAGTTGTTAATGTTTGTGCATGTGCATCCACTGATGATGATGATTGAAT +TTTATTCAGGATTTGAAAAGCTAGGAATTGTAGCTCAGAAATGAATTGATCAATGAACGTGGTAATCGTG +GTGGTGGTGGTGGTGGTGGTGGTATTGTTGGTTGAACTAGTTTGAAGGAAATAAGCTTCAAATTTTGTGA +CACAATGATCCAATTCTGATTTCAACATTTCAGCGTTGAATTGTTTCTTATGGGTTGTTGATCTTGACAG +AGATGATGATGATGATGATGATGACAATGATGATAATGATTCCCGTGGCTTTGTTGTCGTAGATCCAAAA +AGAGTAGGAACGCTATATTCTTCTTCTTCCTTGATGTTAAATGAGGAAACAACGTATTCTGAAACTGTGG +ATCTTTGAAACTTTTCAAACTCCAACAACTGATACGCCACTTTTGTAAGACATGAAATGATGGATTCTGA +TACTGTTTCGTCTAACCAACAAGTGTTTCTGACACAACAATTATCTATCTGTGCCTCATCCCCTAATGGG +CTATTTTCATCTAATGACAAGAGATTTAAAATGGTATTCATTTGCTCAATGAATTTCAATTCCAAATTCT +TTTCAAAGTTTAATCTTTTTCTATTATTGTTAATGTTGAGTTTAATGAAGGTCATCATTTCATTCAAATG +ATGATGTTGTTTGATACCATTTAAGCAAGTGTTCAAGAATAACAGTTGTAATGTTCGCTTAAGGAAAGTC +TCTGAGTTGAAAATCTTGACATCAATCAAAGAACAAAGTTGGGCCTTGTTGAAAACAATAGTTTGTTTAA +GATCAACAAGATCATGGCCAAAGTTGTTGTTTTTAATTATATTGGATGATTTGTTCATGATGAGGATGTT +GAGGTTGGAAGAGTATGTCGAGGTATGCAAAGTTTGATTCATTTCGTTAATTATGTTGTAGTATGAGGAC +AAGTAGTCATTAATGAATGTGGAATAGTTGATATTGATGTGTGGATAGAAGAACTTGACCAGTATCAACA +GTTGAAACTTGTTTCTTTTGAACAGATCCTGAACGTTGAACCGCAGATCCATCCAACCACCACCACCACC +ACAACCAGAGATGTTCTGATGGGTATACTCTAGCATGGTAGTGAATGCATTAAGAGACTTGCAACACTGG +TTGAGTTGCTGTTGAAAATGAGAGATGGAAATGTGTTCTTGTTCAATCACCAACCGATCATTTCTCACTT +GCAGATTCATCGAAATGAAGAGACCATCTGAAAGTTTGATCAGATTCAAGTTGTTGTCCTCCACTGCTAC +TATTGGTGTTGTTGTTGCTCTTGTTGTTGTCATTGAATATGAATGTGAATATGCTGCTGCATAACTGTCT +GCCTCATCATCACCTGAACTACTCAAGTCAGTGCTGCTTTGGCTGTAGAAATGTTGTTGTTGTTGTTGTT +GTTCCGCCCTCCAATCTTCAAACGAGGGGAGTTCCAATTTCAGTTTCAGGTCATGGCAGAGTCTGTGATA +GTGCCTGCTGAGCACGTCAGCCTCACTTCTGATGCAGTCGACTTGGTAAACAAGGTCCTTCAAATTATCA +GATCTCTGTGGCGGCAGCACATAATCAATCTTACACTGGATCTTCTTCTTCTGGCAGGACGAGCAGGGGT +TCGGCGAGCACTTGAAGGCGTCGCATTTGATCTTGTGCTTGCGACAGCGGATGCACGACTTGAGAAGCTT +TGGTTTGGGGAGAACCAGTTGTTGATCGAGCTCAGCGGCAGCGGCAGCGGCTGCTGCGGCACGTGATATG +GTGGATGTCTGTAGTGGCGATTTGGGCGTCTTCTTACCCATCGAGTGTTGTGGCATTTCTATTCTCTGCA +CCAAAGTCCCCAAGACAAGAAGAATCTTACTCACGCAACACACACCTTGACTTAGCGAAAGGAGAAAGAA +TCCAAACACTTTTTTAAAGGGAAAAAGTAAAACAAGCTTAAGCTTATATTTTCCTGGCTTCTCATTGGCT +CAAAAAGAACGGCCTGCAGCGGCTCACAGATTTTTTTTTTTTTTGGTTCCCTGTTCCCCAGGTTCGTTAC +GTAAGCCCCCCGTCCCCCCGGCCCCCGTCAAAAAAACAAATGGAAGAAAAATCTCTCGGACTGCTACGGG +TGGGGCGTCTACGTTTACTTGATTGGGAACTCGGTCGGGAACATGGGAAGTTAGTTAGTTTTATTGGACT +AGTTAGTTAGTGAGTGTTAGTCCGCATGCTTTTGACCTGCCAACAAAATCACAGTGAAAGATTGCAGTGG +CGTGAGAGAGAAGAGAGAGGGACCTCGCACCCCCAACATAGCTATAGTCTACTCTAGCTAGAGTCTACTT +ACCAGTTGTGCCGAGCCAGGGCTGACCATCACTTGCAGTGACTGGCATCCATCCTGATTCCTGATCTCCG +CGAAACAGCAGGTGACGGACGATTCGATTTTCTGCCGTTGTCTCCGAGTTACGGACAGGGACGGAGAACC +TTCTCAGCTCCTACCTGCTGCTGCTGCTCCCTCCCAGTGCTGCCTGGAGGGTTGGAGGGTTGGTTGCCCC +GGGGGCCGGGGCCTCGATGAGATAAATGTTTTTTGAAAGATCCGAACTTCGGAGACGGAGACTGAGACAA +TAAATAATAAATGCCGAGCTTCTGGGGGGGACTCCGAGATTCGCAATTAAGGTACGCCCAAAAAAAAAAT +CGACATACTGTAAAGGGGTCTCTCTCCTCAATCTGGTTTCAAGTATTTATGTAGTACTTTTGTTTAAATC +TCCATCTGCAAGAATCTATCCTCAAAAGTTCGAAGTTATATGTCCTTCGGGGTCCGACAGCAGTTAATTG +TGTCACTTTGACTTACGGGCCTGGCCGCTGGATTCTGGAAACGGGGTCCTGGGATCATAATTTATAAATA +TAATATCTTATTGGAAACTAGCTGAACCCCATCACTGGAGTTCGAACTTCGCAATCCATTTCAAACCAAA +CTTTTTCATTTACCCCTTAAAGTTTACATAGTTCGGAGTTTCATTCTTTTCAAATTCACCTATGAATATA +ATTCATATATCTTAAGCTCTATCTAAAAATGTCGGGTTCTGTCTTCTCCTTCTTCTAATTCTACTAATGG +GTCTTCACTCTTCCCATTACCACCTGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCT +GTTTCCTGAACTGGCGCGTGGTTCTTCCTCCTCCTCCTCCCATCACATTCCCTCAATAACATCATTCGTG +TCACATTCGGTGGTATCATCTCTTGTTGTATCATTATCATTATCAGGACACTCATCAATGGAATGATTTT +TCTTCTTTCTGCTTCTCTTTCTCTTTCTCTTTCTCTTTACCCCTTCAACACCTTCAACACTTTCAGCATC +TTCAGCAGTTACAGAAGATACAACAGCAGTGGTGGTAGGTGTGGTTGATTCTGAACTATTATCCTCAATA +TTCATATACTTGGAAACCAAAGGTTGACCACGCACAGCGACAGTGAAGGTTGCAGCCTTGCTATTATTGT +TGTTGTTATCTCTATTCTCAGTTTCTACAACAACAGTAGCATTCTTGGTGGTGGAATCATCTGTAACAGT +AATAGGCTCATTCTTTACAGTTGGTATTACTATGACAGGGTCTGAATGGGATGGTTCTCTTGAAGTTTGA +GATGAAGATGGAGTGCTGTTGATTTCATCAAAGATTTTCTTGGGATCAGAATTAGAACTCATACGAGATG +ATGAATTTGAAAAAGATTCACCTCGTTTGAAGTTATTAGACTTTGACTCTTCCTGTCTCGAACTAGGTCT +GGAATATGAGCTATGACTCTGATGCTGATGCTGGTGCTGGTGCTGGTGCTGTTTCTTGATTGAGTCTAAT +GGAGTGATTCTATCCTTTAATGGGATTGCACTATTAAGTTCCTTACCCAAAGAACGGATTCTTTGCTCAA +CAGTTGTTGGTTGACTTGATTTTCTGGATAAAGAAGAAGAAACCTTTCTCTCATCTCTCTTAGCAACTCT +GATAATGCTTGGCTCTGCTCCTGCTGCTGATGCTGCTTTGGTTGCTGTTGTTGTTGTTGTTGTAAATGGT +TCCCTTTCAGCTGGGTTCTCATTCATGTACTTCTCCAACAATTCACGGGTATCAATCTCTGCAACTCTCG +CCACCTCATCAAACAACTCTCTTGAGGCATCAATCAGGGAATTGACATCACGTTCATGCTTGTTTGAAAT +ATTGATCAAACGATCAATTATTTTGACGTATTGATCATTCTTCTTTATGTATTTCCCCTCCAATTCATTC +GATCTTAGGGTGATACTCTTCAATGATCTATAAATTGTAGTGTCACTCTTGTTAATCAACGTGATGTTCT +TGTGGATGTGAGAAGCACGCTCCTCGACAAACGTAGACAGTTTCTCCTTCAACTCTCGTACTTCCTTATT +GTAATAATCACGGAACTCCTTCTGACTGCTGACAACTGTATCATAGGAAACTTGCAACCTAGAAATTTCG +GCAATATTACTCTCCAATTCTCTCTTGACACTATCAAAGTTTTTCAATTTGAATGCTTGATCATTCGAGA +TAGACTCCAAATTACCCAATTGTTCCTCTAAATCATTGATCCTACGCTCTTTACGTTTTAAGAGCGAGTT +GACGGTATCATTCTCATGTTTAGAGTTGGACAATTGTTCCATCAGCTGATCTCTGCGTTTCTTCAGTAAC +TCGTTACTCTTTTTAAGTTCTTCATTGTTGTTCTTTAGCCTCAAATTCTCGGATTTCAACTCATCGGCTT +CAGCATGCAGTGATTCAATGAATCGCGAGGATGTGGATGGTTTGGTGGGACTCACTGCGCCATTGCCATT +GACATTACCATTAGACACTCTCAGCAGTCCCAGTTTCACTGGAGACGAATTGACTGACATGTTTGGGAAT +CGGCTTATAATTAGTGAGTGTGGACGTTTCTTGATCTGAATGAAGAAGAAGACTTAATGATTGAATGAAA +CACTCGGCAGTGTCGATCGGTGAATGCCCTGCTTTTCGAGACGCAATCCCTGATAGACCTGTCGTTTCCT +TTATTCTCGTCGACGGTCCAATAACGCAGTCAAAACTTCAAACTCGAGAGTTGTTAATACAAAATGTTGT +TTGTTTGTGGTTTTGTTTTGTTTTAAAAAACTTTTTGTCGTTTTTTGTCGCCGTTTTTTTTTTTTTTCTT +CGATTTTCGTTTAGTTTTTCCATTGTTTGTTTTTGTTTTTGATTTTGTTTTTGTTTTTGTTTTTTTGACG +TTAACTGTGACAACGATGAAACCGTTTTTGAGAATGTGACAGATCAGATTCGAAACGATTGGCTAATTAA +AAAAAAAAACACTTCTTTTTAATGATACACGCTCTCTGATCTGAGCATGCCTAGGTTTCTCAAATTGGTG +AATTGATGATACTCAATGTTCAGAAGGACACTCAATGAGCGTGTTCGGAATTTCTCAAACCGTCAAGTTC +AACCAACCAGTGTTTTTTGTCCACTTCAAGTATATACTCCTGAGAAAAGACATTGTACTACGCTGTGGCC +CCGAGGAAGAGAAACCGGAGTGTTTATGGATTATTGGGACTCATGCCAGCGACAAGTGAATGGTTTCTCT +GGTGGGATCTTCAAGAAGCCTGATACCAAGAATGAAGCTGACCGGTTTGTTGATGAGAATAGAGAGAGGA +TCATAAGCATAGATCTAATACTGGTTCTTCTGATCAATCAGAGAAGAAGAAGAAGAAGAAGAAAGATAAA +TGATAAATCAAAGATCATTGTATACCATGACCGTACCATCTTCGTATCCAACAGCAAGGAAATCACTTTG +AATCACTGTTAAGTCTCGTCTGTTTCTCACTTTCATAAGATCAACACGATTGATGGTTGAGCTACCAGTG +GCCTTTTTCCTCAATGCCAACACGTTCGGTTTGGTGATTCTTTTACTCTTAATCTTGATATCATCCTCTT +CGTTCAAATTTCCGACAACATTCAAATTTCCAGTAATCATCCCTCTTGGTTTCTTGAAACTGGACTCATA +AGTCAACTGTAAAGAAGTGTCAGCAGCAGCAGCAGCATTGCTCCCATGGTAAAACTTAATAAATCCATTC +CAGGATGCAAAGGCATACCTATCACCAATTACTCCAACACTCGCAATCTTACCAAACCCCTTACCTAACT +GCACTTGTGATGACGATTTCCTGCTTCCAACCTCATGAATCACAACTTCGGATCTGATTGAGGTGGAAAT +AACAGTATTATTGGACTCATTATAAGTTAATGACAACACTGGATCAGGAAAATGATCTTTAGCAATGGAA +ATAATCTTGAATTGAGATCCGAATCCATCATCATTTCTTTCAAGATTTAGTTTAACTTGAACGACATGTC +CTGACTCGTATCCGATAAAACATGTACCATTATTACCAGCCCATAGAAGCTTCATAATGATTCCAAACTT +CCCACTTCGTCCCAACACATCGTCATTATTTATTTTTTTTGTCTCATCCCTGGTGATCCCATCTGTGTCT +GTTTCTGTTTCTATTTCTGTTTCTGTTTCTATTTCTGTCATTTGGGTATAGACATCCACCCCTTCAAACA +CTCTCTTTAATCGTCTCTCATCAAGAACTTTACCATCATCATCATCATCATCATCATCATTACCAGCCAA +AAATGAAACGTCATAAACATCAAATCTTTCAGACTCCCGGGTATTAGGAGTCACTAGCCAACAGTCCCAT +ATATCCACATTGCTGAAATTTAATGCATTAACAGGCATCTCATAAACAAGCTCCTCTTTCCATTCACCAC +CATCATTACTTTTCCCATCAAATAATCGCCAGAATTTGACACAGTGATCACGTCCATGTGTAAGAATCAG +TCCAAAAAGTCCACTTCTTCTGCTTTTGCTGTTTTTATTGTCATCGCTGTTATTAACGAGTCCCATCTGT +CGCACAGACATGATCTGGGACTGGTGTGCCTGGATGGAGAGCAGGGGTCGATGTGTTGCCAGATCCCAAA +CGATGAGTCGTCCTGATTCATCGACGGAGATGAGATGTGGTTTCAGCGTGTTCGTGTTGGTGTCGTTGGG +CGGTGCTGATGCTGATGCTGATGCTGATGGTGGGGATAAGGGAACATGGATGAACTCTAGTGCGGTTATG +GGGTGTGTGTGTCTTCTGAGAGTGAATGCCGGGGCTCTGGTGTCTGTTGCAAGAAGGTCCATCTGTTTTG +TCTGCAATTATTCTTTGATTGATTGATTGATTGATTTGATAGTTGAAAGGCTGAAAAGTAAATAAATAAA +TAAACAGAGGAGTCTCGAAAAAAGAAATTCTTCTTCTTTATTAGTGAACTTTAAGCTTTATTTCTAGTGG +TCAATTGGTTCAGCCAACTAACCATGTGTTAGTAAGAGTGGGTATTCTCCACTTTCTCACAAGACTTACA +ACTACTACAAGTCCTAGAAATCAATGGTTTCTGCTTCTCTTTTTTTACTACCGAATAGTCCGTCGGATTT +GATCCGGTGGTTACTATGATTGTGACACAACAATGAGTATAATAAAGTAACGACTAAATAATTTGTAATT +AAAATCAAGGACCCAAGTTCAATGCTTCTCTTTGAGATTTGGACCCAGGAACGAACTTCTTAATGGATAA +CTCAGACGAAGATACTCTTACGGCATGTCTTTCTTCTTTAGAATCCAAATAATCGTTGATAGATGCATGT +TTAGAGATTCTATTGTCATAAATACTGTTCAAGTACTTCAAAAAATACGATCAGTATTCTTCTGGTTCCA +ATCCAACAATCCGCAAAGTAGACGAAGAAGGGTTTATAGACTCAATGCCCTTCCAACCCCGTAACTTCAT +TGGTGATCACTATTGGTTGCTCACGATAAATAAACTCAGGGACCAACCAGCAAGCAAATTGTTTTTCTGT +CAAAGTATTTATGACTATTTCTAACAACAACAACAACCTTCTTCCCCATCGAGAAATTTCTCGGAATGAT +GGAGAAAAGTTTATCATAGGCACCATACCCAGAAACCCCAATAGGTGTCACCTCATTCATTGGGAATGGT +ATCGATGTGTAGATGGGTAATACAATACCAGCAGGATTTACTTCATGGACCAAGTCAGTATGAAGACCAG +TAAATACACACCCTTTGTATAAACGGTCTCCTCCTTTCTCTTGTTGTACCTGGAAGACCAGGAAATATGA +GCCACCATTGGATGAAAACTTCGACATTGTTAGACGACTAGAAGTTACCAATCTCCAGTTGTTTCTATGG +AGATAAGTCCTGGTTGCGGAAAGAAGACAACAAACACGTTCAGCAATCAACCAATGCCAATTCATCTAGT +TCTTGCTCGTTCAAATCTTGGAGTTGAACGCCAATAAGTTCAGTTCCAACGTATTTGGTCAAATGTCTCA +CTTGCTTCTTGGACTTAGCGAACAGTGCCTTCTTTTCCCTGTCGGCATTCTTTCCTCGATCAATGTGTAT +GCGCCCCTCATTGGGTTGATCTCTGATCTTGTAGGCATCATCCAAGAAAAGAGGAAGAAGAGGAATCTCT +TGCGGAAGTTCAGGATACCCGTTAGAAAGGTCGATCCCCCCCACCGAATGAGACGGTAAAGGGAAGCCGG +AAGCCCGGAAGCCCAATACCACTTTTATACTCTCATATATCGCAAAAGTTGTATCCTGTGTGACTAGCAA +CTGTGGATTGGACCCTTGCAAAATGCCCACAGTACATCGATATCAACTGGCTACTTGTCTCGATTAAGCG +AATGTGTGTTTCGGTGTCGGCTTTCTTTTCGGGGCTTTTGTCATTTTTTTGACACAATTTTCTAATTGGT +TGTGGTTTCTTCTGGCTTTTTTAAGCGGGCGCTCTAAAGCGAAGGTAGTGGTGGTAGTGGTAGTGGTAGT +GGTAGTGGTCGTGGCTTCTGACGCTGACAAAACCTGCTTCTTTCCTCCCTCTCTGGTACAGATTATGTCG +ATCAATGCTTCCTCCAAAGTGATCCATCCACAAACATAAAAAAAAAAAAAAAAAACTGATAAAAACTCCC +AAAAATTCAATGATATTTACTATTAGTATACAAAAGGGCCGTCCGTAGTTTTAGTTAGTTAGTCCCAATA +AACCTCTTTTTATGTCGAAAAGAAAAGAAAAAAATGCAGATCTTTTTTTTTTAATGATGTTCTTTGGATT +TAGGCATATGATGCGTCCTTGTCCGTCCTCGTCCTCGTCCCCAACCTTAACCAAGACCAAACTGTTTCAT +CATGTTTTGAACCTGTGGATTGTTCATCATTTGTCTCATATCTGGCATTCCACCAGGACCTCCTCCTCCT +CCTCCTCCCATCATCTTCATCATTTCGCTCATGTCGGGCATTCCTCCTCCCGCTCCAATACCACCAGGAC +TACCAGGACCTCCAGCACCCATCTGTTGCATCATTCTACTCATTGCAGGGTTATTCTGCATGTTTTGCAA +ACCTCTTTGCATTTGAGAAGACTTTTGAGCATCCATCATACTCTTGGAGCGTTGTGCCATTGTAGCCATC +ATTCTTTGTTGCATTAGAACCATTTCAACATCAGTCACTGTTGTACCGGAACCTCTAGCAACTCTGACGA +TTCTCGTAGGTTCATCGATAAAGATTTGACCATCACTATCCAATTCCTTTTTGGTCATTGAATCCATGAT +GTATACCAAACTTTTCATCTTTTTGGATGCTTCGTCAGTACCAACTTGATTCAACATGTCACCCATACCT +GGAATCATACCAGCAATCTGTGATAATGGACCCATCTTCATAATGTTTTGCATTTGTTTTTGGAAATCAT +TTAATGTGAATTTACCCTCTTGAAAATTCTTCATAGTATCCTTGTTATCCAATTCAAGTGATTGAACGTG +TTCCATAAAGCCTTGAATATCACCAATTCCCAACAATTTAGAAATGAAACTTCTTGGAGAGAAAGTTTCA +AAGTCTTGAACATGTTCACCAGTACCAATAAAGACAATTGGAGTCTTTGTAGCTGCAACGGCAGAAATAG +CACCACCACCTTTGGCATGACCATCTAATTTGGTCAGTATTATGGAACCAAAGTTGGAACTCTCCTTAAA +TGCCTTTGATTGGGATTCGGCAGCTTGACCAATGGAAGCGTCAAGAACCATGATTGTTTGGTTTGGATCA +ACAGCTTCACCAATCTCAACCATTTCTTGGAAAAGATCCTCCTCTTGTCTGTGTCTTCCGGAGGTATCAA +CAATAATGATATCAAACTTCTCCTTTCTAAACTTATCAACACCATCTCTTGAAACTTTGACTGGATTCGT +CTCAGTATAAGAACCATAGTATGGGATTCTTGCCTTTGTTGCATTCTGTTTCAACTGGTCAAAGGCACCA +GCTCTGAAAGTGTCTGCACAAACCATACCGACTTTATATCCTCTTCTTTGATAGAAAACAGCAAGTTTAG +TACATGAAGTGGTTTTACCAGCACCTTGTAACCCAACAAACATAATCACGTTCGTCTTGCCCTTCTTTGG +CTTGAATGGTTCGGATTCGGTATCAACTAAAGCACACAGCTCGTCAAACACCACTTTCTGAACAAGACGA +CGCTTGTTTGACATGTTGTTGTTGTTGTTGTTGGAAGTGGCCGTGGCCTCATCTGCATTTAGTTTGGCTT +TGATGTCCTTTCTAAGTTTGGCAACGAGTCTGATGTTGACGTCGGATTCGAGCAGGGCATTGCAAATCTC +CTTCAGCATCAGGTCGATGGCGGACTCTGAGACATCGGAGGACTTGACGGCGCTTTGGATGGCACCGTTG +ATGCGCTTTCCGAGGTCTGCGAGCACCATCTTTCTTTCTTATTCTTTCTTTCTTATTCTTTTTATGGTTC +ATTCTGCTTTTTTCTTTTCGGGTTTTTCCGAGGACAACTTTTTTTTACTTGGATTCGATGGTTACCCGGT +TTGCTAGGCTAATATCAAATCAAAAAGCATTATTAGCTTCACCTTCTTTCTTTCTTTCCTTTCTTTCTTT +CTTTCCTTTCTTTCCATTTGCTTCCTTCCTCATCTGAAGAAGCCAACAATGGACTTCTTTCTCACGCGAA +CAGACTCGTCCGTGACTCCCTCATCGCTCAAGTACTTCGCCATCTTCGACCCCACGCTGTCAAACCATGA +TGGAAATGATGGAAATGATGACGATGATGACAACGACTCAGAGCAGTTGCTGGTGTATCTCCCGACAGAA +GACTCCGCAGATATTCATACAAAGATCAGAAGACTAGGGCTGCTCACCGGTATTGACGCGTTCTGCTCTG +GATTTGACAATGACAACAACAGTGAACAAGAAGAAGAAGAAGAAGAAGAAGTAAATCCCTTGAAATGTGT +GGAGTTGGAAGACAGCAGGATTCTCTTTGAGACGGTTGAAGGCCGGTACAACGTCGTCATTGCGATCCAA +TTGCCCAAAATCGAGGAGGATGACGGCAAAACCACCACTTATGATACCAGTGTTCCCCCGAGTTTCTACG +CAGACCTCATAATAGAGGGTTATAACGAGTTTAGACTGCATAATGGAACAATTTTATCGCTTTTTGAAAC +AATGGAAAGAGAAGAGTTTGTCAATTATTTGGAGAGATGGTGGACTGTATGGTATAACAACTTTCAATTG +GATTTCACAGAAGATGGGTTTATGAAGCAATATTCACAATGTTATCATAAATCCTTTTTAAAACTGGATA +GTGAAATGAAGTACACCGTTGACAAAATCCATGAGTTTGCCCAATTCAAAGAGTTTCTGGTTTGGAATAG +TGAGAGCGGATCAGAAAACTTTGGATTGTTGTATGAACATTACAAGAATCTCCACCATGAATCACGAACC +CGTTTGCTCAAATGGCTGGAAATAACATCCCACTATTCGCTCACCACCGATTCATTGTTAGCCAATAATA +TCACCAAGAAGATTCCTCCTGATGAAGCCAATAATAATCAGACGAATGAGAATGAACTGAATCAAGGTGT +TGAATACGTATATGACCCCTTCAAGATTTTGTTTAGCACCATCACTGATGTTTCTAAAAAAACTGGCATC +ACAACTGGTGTCAATTACAGTGTTAAGACAATGTCTAATGGGTTTAACGCAGTTAACAACTACATTCCTA +CTATTACTAGTCCATGGGGTTCATCCAGCTCATCCGTACCTCCAGTTTCTGATCTTCTTCTTCTTCCACC +AGAGGGTTCAGAATCCATACCTCCCAAGCAACAAGTTAATGATCCTTCAATTCCATTATTTCTCATTGGT +ATCAAGACTAATGAGACTTCAATCACGTACAAAAACCTTTATCTTAAGTTCCAAGAAGAAGAAGAAGAAG +AAGAAGAAGAGCAGCTTTACAGAATTGTGATATACGCATATGAAAAGCAAATCCTATTAACAATTTATCC +GTTCAATGCCAATCAATTAAATGATCCCAGCTATTATGACACCTTATACCAAATGCTAACAAACTTATCA +AAGGTTCATTTCCCCAAACAAGAAGATCTCAAAAATAAGAAAAAAAACATTCCTCGAAGCTTTTATTATG +TTGGTATTGATACCGAATCACAAACTATTGAATCAAATTTGCCATACATACCAAGAACAGATATCCATGA +ATCATCAGATGATACAGTTGATGAACTCAAGATTGATGATTTGGCAATGAATAGGGCTAAATGTCAATTG +ATTCACAAAGAGTTAACTGCATTATTTGGTCAAAGGGATCGTGATGAACTCAACGCTTTAAAAGAGAGAC +TTAACATGACTAGAAATGGTTGGTGGATTTATAATCTCAACAATAAAGTCAAGAGTGTTTTTATTTTAAA +AAAATCGAAAAAATCGCAGTCCAATAACAACTCCAACTTCAACTCATCATCACATTCTGGATTGCTTGAT +ATTTCAGCTAATGTTCATGATATTTTGGGTAAAGATGCTAATGAATGGGCTAACAACTACATCAATGGAT +ACCTCAACTAGTTAGTTGTACGAGTAAATAAATAAATTAATACATTGAACAGGGAACAGAGAGATGCATT +TTTTTTTATACATTTATGTGCTGTTCTTAAATGCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCCA +ACCCGTCCCTGCTCCCACCTCTACTCCTACTCTATCTTTTTTCACCACTAAATTGATGTGATCTTCAAGA +ATTCAATATGCCCATCAATAAATCCACATAATTCCGTTCTACTAATCTCATTAAAATACTTCAATGAAAT +ATTCAACTCACAAAGCAAAAACTTCATTCTTTTGAACTGAACCATTTCAAAGTCAAAATAATCATCATCA +TATTGATTTGCTTCATCCTTTGGTAATTTGGGCAAAAACTTTGGAATATCACCATACATTCTAAACTCCT +TACCATCATCATTAATCAACTTCTTCTCCGTAAAGAGCAAGTATGGATTCAATTTCGGATAGTTGGCATG +AACTGTGAAAACAGATGCTGCACGTGTATCGAAATCTTTAAACTTTTCTGCACCATCTAAATCAGGATTC +AACTTTAAAAACTTTTGAAATTGTTCATAATCTTCAATTGGATTTCTTTGACCCATAGAAACACCGACAA +AATCTCTCGAGTTTGGTTTAAACCAACCAAAAGATTGGATTTCTGACTTGATTTGATAAAAAGTTTCATC +ACCTCTTACCGCAGCAGCAATAAATGTGTCTTTATCACCTTCACCAGAGTTACCTTGTGTCAATAGGGCG +TAGTAAAGTTCAGGGCCATATAAATTGTAAAACAATGACAATAAAAGAGTCTTAACGTGAGTCTTTTTAT +TGATCATCAATTGACCTGATTCTGAGGAGATATCAGGAATAGCACCTTTCAAATCATGGAATTTGGCATC +ATTGACACTGGCAAGTTTCTTATGTTGGAGTAGTGGCCATTTACCCTCTCTAACCTGCAAGGTTTCATCA +ACCTTTTGACCAACAATGTCATAGAAATATGGAGATGTGGTTCTTTCCCAATAATCTGGCCAGATTAACA +TACCTGTTTTTTTATATGGTTCACTATCGAAAAATGATTGTGGATCAGTCACGAGTAAATTGTCAGAATC +CACTAAGAATACATCAGCAAAACTAGATGCAATCAATGCTAGAGATTTGTACTGATATCCAGAGAAAGTC +ATCGATTGAACAACTTGTGGACCCAAGACGTTGAATAGAAGAACACATCTGGCATCCAGTGCTGGAAGAA +TTTCTTCACATAAATCATGCTCATATTCTTCTTCAGTAGGTATAATGACTTCAATCGGGAGAGTCGATCC +GAAAGATCTAATTGATTTGATCGAAAGTAGTGATAACCACGAGTATTTCTCACCACCAATCAGTACGATT +CCCTGGCCTTTATAAAGACCAGCTGGAAACTCGTCTGGAATGGCATCATAAATATCCTGATGGATCTCAC +GGGCTGTATTTAGGTCCTTTTCCGGGATACTCATGTATCCCTGCAACTTCTCCTTATTCCAAAAAGCAGT +ATCTCTTTGCCATTCAGTACGATCCAGAAATGTCTGATGAGGCATAATCGTTCTCTTCGATTCCATTTGC +AACATCAGATGCTCATAAAACTTTTGAAACTTCTTGTTGAACCCATCCTCATACTTCTCATGGGTCTTTA +CACCTTCCTTGAACTTCTTAGAGTCACTGATATCCATGTTAGAGTTCACATGCTGTTTGAGGAAGGCATC +ATTGTCATCATCATTATTATCGGTGAAGTAATCTTGAATCTCCTGTGATATGCTCTGGAGTGACTCTGGC +GACGCGTTAGGGATGGATGTGAGGGAGTACAGCAGTCCTGAGACCACGAGCAAGAGGATCAGCAGCCGTC +TGACGCGGGGCGTAATGAAACCGAGCATATTCGATGCGTCAGAAATTCAGGAAATCCGAATCGATTATTT +GACCTTTGATTTTTGATTTTTGATTTTTTGTTTGTTTGTCATTTTTTTTTTTCTTTTTTAGTGTCTCTGT +CACGTGCCGTTTGGAGTTTTTTACAAGCTTACAGCTACTACGTTTAGTCAAAAAAAAAAAAAGTGGGGAA +GAAAGAATCTTTTACTGGAATATCCATAAGCTAAATGGAGAATCTTTTGTTAGTAAAACTGTCTCTATCA +ATGTCACATCACACCAGAAAAGACACAACAAAAGAACTTTGGAGAACCATTGGAGTTATGTTATATTATC +AGAATCATTAAATCAGTATTTTTTGTTTAGTCGTCATAGGAAATCGGACAGTGTGAATATAAAAAAAAGA +TCATCACAGAAAGGATAATGGCCCCAGTTGCTTTTGTATCATCTCGTTGGTTTGAGTTGGAGAGGTGGTG +TATGTATGGACACTTACTTCTTTGAAAACATGTTAGCTATTACTCCCTGGTTTTTTTTGTATTGAAGCGC +ATTGACAAAGTTCTTGATCAAAATTCCTTGCATGTCAGCTAAAGACCGTTCATCAATCTCAACCCACCAA +TGCCCTAACATTTGTACACAAGGACACGACTACCAGCAGAACTGGTGGTGGATCAAGCACTCTATGTCCG +CCCGGTCGAAATGGATCATCATCCAGAGAAATTTTGATCAAAAAAACATCAGTTTGAGAGAGAGAGAACT +GCATCCCAAAAAAAAAAGAACGTACTGGAAACCAAAATGTTAGAAAAAAAAATTCACTCACTCACCCAAT +TATGGAAATGGCTTACCAACAACAAGCAAAGCAACACACAAAAAGGGGAAAAATTCAAAAGAAGAAGTAG +CTCATACAAGGCTGAAACTTAACGATAATGCAATTGTCGACATATACTTATTCAGGTGGAACCAGTGAAA +CTGAAAGCAAATAATCACCGCGACCTCTTCAAATGAATGTCCTAATTGATGAGACTTTGCTTGTCGGAGT +GGAAAGGAAAGATACACACCCTTTTTGTGTTTTTGCACAGACTAAAAAACTACAGAATGAAGCCACGGTT +TCATTCCGTTGATCTCTAAGTCGTTTATCGTTCAGGATATTATTGGTAGAAAGAAGGAGTGAAAGCAAAA +AAAGGTTAAAAAATTTTTTTTTTTTCCCCGAGATTTAAATAAAAGTGAACAAAAAGAGTTCCGAGGATGG +GAAGAGACGCAATGTCACGTGCTACATGTGACCTCCAATTTTAATGTTAGGGTTTCAAAAAAAATTTAGG +GTTGTTGCAAAAGTTAGAAGTTTAACCAACCGAGTCGAGACTCTTGCCTCCAAACTCCTTTGCATTTGTA +CTAAAAACAAAACAACTGTTTGAAACAAGTTTGCAACTGGAAACTGACTTTGCTTATCCTCTTTTTTTTT +AAGACTTTAGACAACTTAGCTTTACAAGTAGTAACGCTTTGATCACGAGTTTTCGCTTATTTTTGCGCGT +TTTTGTGCTTATCGTCCGTCACAAAAAAAAATTAAGGGTTGTTATCAGTGCCGCCGCGCTCTTCAAGTGT +CCCCCTCCTATCTGAAGCCTTATACTAATAAGAGTCAAGACACGCGTGGGTAACGAGCCTTTTCTTCATG +TCCCTTTTGGTGATAGGAGAGAGAAGAAAGTGATCTCAGTCTCACTAATTAATCATGTTTAATGTACGGA +CAACAGCGGTTCCCTGTGCCTGAAGCTCTTACTATGATTTGAGAAGGTGATTACTAGTGACTAGCGACTA +GACTCTAGATTCTATATAGTTTACACCCTTATAAATGGATCAAATGTCAAAGAGTCGTGTGACAGGTTTG +CCTCCGCAAATGGGTCCTCAAATTATTCCCATCAGTGACATGGCTGACGGGAGGGCACCGATATGTAATC +GCATCATCCCGCATCGAACGATGCCTATCACACTGTGACATGTGTGTCTGAAACTCTCTCTCTCTCTCAA +ACAAAGTTTTTCTTTCCCCTTTAGAGAATCTACAGCCCGCGCCGCCTATCAATTTTTTTTTTCATGACGA +TAAGCCACATGTTTTATTATTTCTTTGCCTTACGGTATGCCCCATAGTTAAGAAAGAGCTTCACTAACAA +AACATCCGAGACGCTGATTGTCAGATATCACCAGCCAATAAGAGCAAAGATGAATTTAGATTCGAATCCT +CCTCATATCTACATTCTAGATGTCGAAATATAACATTGATAAGATGGATGGATGGGATGCCATGCAGCCA +TAAGATCAATTGCGGCAAATAGTAAACAAAACTAAAACTAAAAAATGGGGAAGTGAACGTGACGTGTCCG +TCCGTGACAGGGACACGTAAATTTCCATCATCATTTTGCAAGAACTATCTATGTACGTTATGCTATCCAT +CAGACCTCCATTTTCCTTTAGAGCTAGTAATTCTTTTAGGGCTCACACAAGTTGAAAAAAAAAAAACCTC +AAAGCAAAACCATCGATTGCTACATGATTGCTAGTATCTCTATCTGCTGGATGGTTTATTGACTACTCAA +ACTCAGAATTTGACATTCAAGAGTGACGTAAGTATAGTTGCAATAGATTCTGGATTAGCAGTTAGAACTT +AGTCCACTTCTTTCAATACTAGTTGAAACTTTGACGTATACAACCACAAATTGAAGGTTTCAAGACTCAA +AGACTCAAGACCTGCTTAATGTCTATCTGATTCCCCAACTGCTCTACAAGAGAAAGGCTTCTGGAAACGA +CTACCTAAAGGGCCAATATTGCTTAGCTTAATATCCATGGATATGGTTTCTAAATCCTGACCGCTCCAAA +AACATTCCAGATCCCAACAAATCAATTCGATCTCCTCTATCGAAGTGAAACATGTGCGAAAAAGGGCACG +GGGTATATTGAATAGATCTACATAGCCACATGCACACATGCCCCACGTTCAATCAGGTTATGAATATACG +CAGCACACGGCTCCAGAGTCCAAAGCCCACCATGGGACACGCCCATGTGCCCAGAGTTGATGGTTGCTTT +ACCTCGGAAGGCATGGCATGTGTCAATTCCCCATCTTTTCTTTTAAGCGCATGTGTCAGAAGTCGAGCTT +AGAAGTTAGGACTCAAGGGGTAAATAGTTAAGACTCAAGAGCTGGCGCATGCGGTTGGAACTGATGTTAG +ACATCACCAGACTACAGGGTCTCCAAACAAGTTACAACACTAGAGGCACGTTTGTTGATTTACATATAGT +TAAAGAAAGACACATGGTTAAAAAGAAAAACCTTGGCACGTTTTTTAGTGAAAGGTTAATTAAATAAAAC +ACACCCTTCAAGGGTAAAGCGGAAGAAACGTTTCCTCGTAACTTAAGAGGTAGTAGTCGTCTTTTGATTG +AGTCCAACATAATTGCCCTTTCTCTTCAAAGTTCAAATAGAGGGGAGACACCCGTATTGGCCTAAAGTCG +GGATCATCCTATAAAGCATGATAAAGGCCTTATCATCGCAGCTATCGGTAGCACCCGATTCACAAGTAGT +CTGTAATATGTATTTTTCGTGAAGGCGGCAAGCACCGAGTTTCAACTACTCTCCACTCAGAACACGATTT +TACTTGGACCTTTCATGAAGATATTCCGAATGGCATAGCTGAATTTCAAGGATGAGTATATACTGTATAA +AGTTGGCCAAGTTATGTACTTTCTCGTGGCGGTTTAGGGCTTCCTCAGCCTTGTTTTTTGAGAGAATTTA +CCTCTAAACGATGGTGTTTGATGAAAACCAAGAAAGAAGATACGTAGAGATAGCGCAGTGTTTCCTTACC +AAGATCTGCGCGCAAGGATGATTTTGTTATTCAGGAAGGTTAGTGTTTTTGGGTATCAACAATCACCCTC +AAAAACCCCGGAATAGCTTCTTTCCGAGGTGGTTCAGCAAACTTGAGTGAACTTTGAGGAGCAGGTTACT +ACTACGAGGAATTGTCCTTCAGAAGTATTCACCCGTAATCTGCTTCAATTGTGAGCCCATTGCACCAACG +TGGTGAATATTTCAAATTTTCGATGCAAGCTACCAATCAGGACTCACTGCTCAGAGCCTACTAGTGATGA +CTGGCTGGCTTGATCACTTGCTCTTGTTTGTGAAACTCACTCGCTCACTTTCAGTTTTCCTTCCCCCTTT +TGATAAAAAAGTTTTTTTTTTTTCTCCTGTTGAAAAATGCAAGGGAGATCTTCAAAAAGTTCAAAACTTC +AAAGTGATCAACAAAAAAGAAAAGCCCCTGGAATCTGGATTCAAGAAAAAGTTCACCCCATTGCCTTAAA +CGAGCAACTAAGGCATGATCAAAGTGGGATCACAAGGGGAAAGGGCAAGAAAGTCTTCAATCAATGAATC +ATTAAAGCGCTAATCCCAATGTGTTCCATGCTCCAGTATCCCTTGAGACATATCCTCTACATATTTTGGA +GGTACTTTCGCACCTACAGGCTACAGCGCTCGCTACATAATACACTTACAGCCTACACTCTACAGCCTAT +AACCGTAACGCTTATTTTAGCGAGATGCTATTGGCTAATTTGATGGCTTCCGCTCATAGGAAGTGGACAT +TGAAAAACTGTAAAAACCGTTGATTCGGTCTTAAGCTAAGTCTTGAGAAATGGTTAATTGGATCAGTAAG +AAATAGGCGGGGGTTTGTAGGGAAGCTCTGCCTAAGGCTCTGTGTTCCGCATCCTAATAAGGTACTAGTT +GTGGGCAGCTACATAGCCGGGTAACCGCGATGGTCCGCACATTCCGCTAAAAAGAATTTTACAGTTGGCG +ATGAAAAAGAAATTTTTTTTGCAAAAATATTTTTTTTTTCTGGCAGCTCATCGCAAAAAAAAAAAAGACC +CAAGTTATAAATAAGGGGTTTCCTCCTCAACAACTGTTTTTGTTTACGTTTGTGATCCCAACAGTCATAG +GTTCTTGGAGTGTTTTTTTTCTGACCAATTCCATTATACCCTTTATTGCACCTCGCGTCCTATTATATCA +GTGTTTAACGAATTGATATAACATTAGAAGAAGCTGGTAGCAATTTTTTTACATCTTAAAGCTCTTCTTT +TTATTTGATTTGTGTCGAAACTCACCAGAACCTCCTTCCAATAATGGCCCCATTAACTCGTAAGTCTAAC +CGTGTTCGTTTTTTGACTTCAAAAGCTAAACATTCTGAAGAATCCCCCACCCGTTTGAACCACAGTCATG +GTACCACCACCACCACCACCACTACCATCGCCTCCTACTCCAAGAAGCCAGTCTTGAGATCCATCTCCAC +TATCGATGAGAACGAGAGTCCATACAACAACTTGAACTTCAAGAGAAGGAAACTACAGACTTCTGCCAAA +CAATCTGCAGGGCAGAAGGTTAATAATATCGTTGAAGAATCTGACGATGGGTACTACTCCACGCCAAATT +TCAGCGTTCCAGTCGATTTCGTTTCCATGAGTGAGGATGAGCTTCGTGGTCAGTCTGAAGAAGACAACCT +TTTCAACTCTGAAGATGATAATGAATACTACAGTGCTATCTCTGATTATGAGTCGGATAGTCTCTTCACC +AAGCCAGTTACCCAGCACAAGAGATTTATCAACCACGCTAAGGCGTTCCAATTTAGAAAAAACCAAAGCG +AGGAGATGTCTAGACTTGCCCAGGTGAAATTTCTACAGCTTGAGCAGAACAACTTGTTTGAGAGCGATGA +GTCTGATTCGGAAGCTACAACTCCTATCAACACTCAATTCAGCTTTGATGATAACTTTGGTGAGTTGAAA +TATCTAGAGGAGAACTTCAAGAAAATTATTGATGAAAACCACAAGTCCTCTCAATTCTCGAACTCATCTG +ATGGTGAATCAATGTTGGAACAGGCAGTTGAGGATCATTCTTCCAAGAATGATGTTATTACCATTGATGA +TTTCGTTGATTACACATCTGATGTCGTCTCCTCTGATGAGGACTTTGTGCAAGCGCAGTCGAGCTCTTTC +AAATCGTCTTCTAGACTTTTGCATTACAGACACGATGAGAAGCATAACTTGGCTTTCCCTGACGTTTCTA +CATTTCCAGTCAGATTTGATCCAAATCTCAGCAGCAAGAAGGCAACCACCAAGAAGTCTGTTAGAGCCTT +GAACAGAAGAGCAATTTTGACTGGTATGGCCAGTGAAATGGTTGGTACTGGTATGAGCATTGGTGAGTTC +AACATCTTTTAATTCTGGGGAGTACACTAGGCTACTGTTTGTTGTTATTTTTTTTGTCCTTTAATTCACT +GAAGAGACACAACACATGATCTTCATCCACTTTTAATGATTTTGTTTTCACTGTCACGCTTTGTTTTTTT +TTTTTACTTCATTATGGCTCCTCCGTTCTTGTTGAGTGGTATCTATAGTTTGTCCACTGTTGTTTTGGCT +TGTTTTTATCTCTTGCATTGGGTCATCGAGATGACTCTGATAAGATTGGGACTATCGCATTTAAGGTTTA +TTGGTTTTTGTTTTACATAATAGAGAATTGGTTTTTCTTGTTGGCTAGAGAGTCAGCTCGTCATTCGTTT +GTATCTAGATTTTAGAGTTTGATAGCTTAATATATCACACATATATGGGGTTGTTTTGTAGAGATATTAG +AGATTTACTTATCCTTTTTGCCCGTTCATATGTTAGAGAGAGGACCACCGTCGTTACCCGGTGAAGAATC +AAAGGAGCATGACTTCTAACGATTTCACAAAAGGATAATTTCCGAATTCGGGCTTTTATCCTAAGATTTT +CCTTTTGCCCAGGCGGCAAAAGTGGTTTGCGTGATCACTTGGGCTTGATCATCACATGCGTGTTTGTCCC +AGTTTGTGGCTTCCAAGTTCTAAACTTATCAACAGTGCAACTGTTATTTGACCACGATATACTTTGTATA +AGTAGATCTGATGTCTTTCTTTTTTTTCGATGCACTTTCCTTCCTTATGCTGCCCATTTAGCAAAAAGGT +TCAAGTCCCCACAGTTTTTTTTTTTTTTCATTTTTTCTTTCTTCTTATTTTTTCCGCCGTTCGGGCCGGA +TATTTTGACAGTTTGATTAAGGGATCTCGTACTTCTCAGCCGACTTAACAAAGAAAATCTAAAATTAATT +AATGTTTTCTTTCCTCTTCGAAACCAAACTCTAAAGACTTTCCGAAATGTTGAAAGCTACTACCCAACTT +TTGAAGGACGCCTTGATTCCATTGCCCGCTGAAAAGAGGCCTCACCGCCGGGGACCCCTTCAGGAGCAGG +AAGAGCAGAAGCATAATGACCCCTTCAAGTTGCCATTGGACCCACAAATAATATCCATCAGTGACGCTTC +GACGTTGGTCCCACAGACACAGAGAAAGCTGAAGAACAG + diff --git a/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.lift.gff b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.lift.gff new file mode 100644 index 00000000..c7567844 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.lift.gff @@ -0,0 +1,14 @@ +##gff-version 3 +##PAF BC_dic1 316 0 316 + CM113344.1:1105885-1164023 58139 34092 35031 732 951 0 AS:i:1226 ms:i:1226 np:i:267 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:8M4I252M1D52M cs:Z::3*accV*tccT*caaA*cctT:1+HSTA:1*cagG*ctgA*tccP*ttgI*cagA*tccL:1*atgP:1*acaA*accS*acaA*accN*acaA*actK*cctT*gtcI:2*gcaT*tcaK*aaaT:2*caaT:15*tgcF*ttcC:25*cttF:4*caaA:3*cccT:1*atgI:11*ggtA:8*gtcA:5*gggE*aatS:6*acaM*aagS:10*tttV:26*cttI*gatE*aagQ:4*gcgK*catN:5*attA:3*aagT*gatE:2*atgP*aatS:29*cttM:2*actS*ttaF:3*ggaK*gatE:6*aacS:7*gtgA:19*tcaG*gcaT-gga:1*caaH:2*attV*gaaH:3*actK:1*atcL:1*aatD:23*accI:2*gcaV:2*caaE:2*catR*ttaM*aagR*gtgI:2 +CM113344.1:1105885-1164023 miniprot mRNA 34093 35034 1226 + . ID=L000001;Rank=1;Identity=0.7697;Positive=0.8423;Target=BC_dic1 1 316 +CM113344.1:1105885-1164023 miniprot CDS 34093 35034 1226 + 0 Parent=L000001;Rank=1;Identity=0.7697;Target=BC_dic1 1 316 +CM113344.1:1105885-1164023 miniprot stop_codon 35032 35034 0 + 0 Parent=L000001;Rank=1 +##PAF BC_MATa1 162 93 159 - CM113344.1:1105885-1164023 58139 28260 28467 126 207 0 AS:i:203 ms:i:203 np:i:50 fs:i:0 st:i:0 da:i:69 do:i:18 cg:Z:8M3D58M cs:Z::2*agcD*actS*accN*gaaN:1*gtgI-aacatttca*gaaQ*acgQ:2*aagR:1*accR*ccaH*atgI:2*gcaE*acaA:1*gcaS:3*gaaK*gtcA:2*atgT*attT:1*acaN:1*aatT:5*gcgI:4*tgtS:3*cctA:13*tcaT:1 +CM113344.1:1105885-1164023 miniprot mRNA 28261 28467 203 - . ID=L000002;Rank=1;Identity=0.6087;Positive=0.7246;Target=BC_MATa1 94 159 +CM113344.1:1105885-1164023 miniprot CDS 28261 28467 203 - 0 Parent=L000002;Rank=1;Identity=0.6087;Target=BC_MATa1 94 159 +##PAF BC_MATalpha1 233 38 211 - CM113344.1:1105885-1164023 58139 29132 29657 243 537 0 AS:i:359 ms:i:359 np:i:112 fs:i:0 st:i:0 da:i:87 do:i:54 cg:Z:6M3D9M1I107M1D15M2D14M3I18M cs:Z::2*gggN:1*aaaS:1-agacggagg*cgtC*tgtT*gacS:1*gcaS*tcgN:2*ggcC+L:4*tccT:3*caaK:5*acaA*aaaD*tgcY*cagE*aacR:2*gcaE*agcG:1*ctgK*aatD*aagH:1*aagA*aagG*aagQ*aagD*aatS:1*aaaT*caaY*atgI*cacR*acaQ*gtgH*ccgL*aagR*aaaR*ttaK*aacR*aagG:1*aaaS*caaR:8*attM:9*attV*tccA:1*gagD*cacQ:2*gacV:2*ttaS*ataQ:3*caaE:2*agtR*agtN:1*ggaE*tttI:2*attV:1*gctK:2*gctS*gctT:4*ttcS*ttgV*tccL*cagS-cac*cccD*gaaR*atgL*acgP:1*gttF:2*attL:2*tcgV:3-gcatta*cagP*gaaT*gctC*gtgI*aatS*agaE*tcgN*cttI*catY*actD*acaN*ccaK:2+GTF:5*attV:2*ccaT*gggN:1*ggcA*aggD*agcE*tcaN*agcH:1*tacF +CM113344.1:1105885-1164023 miniprot mRNA 29133 29657 359 - . ID=L000003;Rank=1;Identity=0.4525;Positive=0.6257;Target=BC_MATalpha1 39 211 +CM113344.1:1105885-1164023 miniprot CDS 29133 29657 359 - 0 Parent=L000003;Rank=1;Identity=0.4525;Target=BC_MATalpha1 39 211 +##PAF BC_sla2 1046 0 1044 + CM113344.1:1105885-1164023 58139 24982 28159 2748 3180 0 AS:i:4569 ms:i:4569 np:i:991 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:97M1D173M6D16M6D286M1D226M2D190M1I55M cs:Z::12*tcgA*gttL:29*aaaL:7*ctaI:16*gctI:3*attL:4*gatQ:4*gatE:14-acc*ttcI:12*aggK:10*ctgQ:17*gtcI:6*gacN:8*ttgM*gaaD:17*gcaT:18*gcaT:15*gttI:17*gatE:29*caaE*tctT:4*ttgM*atcV:2*gatE*gatS*aatS-aactctcctccacaacca*gagV*gacE:2*atcT*caaP:5*atgQ:1*agtN*caaL:1-actaaccacattgagcct:6*ctaP:1*atgV:8*gttA:8*aacS:3*accS*gatS*tacF:1*actA:9*gaaD:2*actA:11*ctcI:14*ctcI:3*aatQ:1*attL:1*gatE:34*tacD:2*attV:7*gctT*accA:1*gctT:6*ttgI:14*aatT:74*ttgV:9*atgK:14*tctA:19*actE*aatA:2*tcaN:5*caaS:2-tct:3*gacE:8*gaaD:5*gttL:8*gctV:6*cagK:2*gatE*tccT:34*tccA*attL:17*ttcY:7*tcaA:1*acaS:2*actM:2*gcaG*aacD:6*gctI:7*tctA:13*tcaA:14*acaS:1*tccA:4*atcF:1*tccA*tgtT:2*tccA:15*atgS:3*gatE*gaaV:1*gcaE:1*atgT*gaaD:2*attM*gttA:3*agtA:6*gaaD:4*gctT:5*aaaR:5*aatS:1-agaggt*gctV:3*agtG:1*attL:1*gatE*aatT:6*caaA:2*gctS*acgK:2*ttgS:2*aatT:3*accS:1*aagD:16*ttaV*gctS:10*attL:48*gttI:11*acaN:1*acaS:2*gaaQ:5*aatH:28*gctT:3*agtN:5*aaaR*ggcS:10*agaQ:5+S:4*ttaV:5*tctT:42*gacS:1 +CM113344.1:1105885-1164023 miniprot mRNA 24983 28159 4569 + . ID=L000004;Rank=1;Identity=0.8642;Positive=0.9349;Target=BC_sla2 1 1044 +CM113344.1:1105885-1164023 miniprot CDS 24983 28159 4569 + 0 Parent=L000004;Rank=1;Identity=0.8642;Target=BC_sla2 1 1044 diff --git a/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.miniprot.gff b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.miniprot.gff new file mode 100644 index 00000000..e67a2238 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_CM113344.1_1105885-1164023.miniprot.gff @@ -0,0 +1,86 @@ +##gff-version 3 +##PAF MH260578_SUI1 109 0 109 - CM113344.1:1105885-1164023 58139 24286 24613 273 327 0 AS:i:493 ms:i:493 np:i:103 fs:i:0 st:i:0 da:i:3 do:i:0 cg:Z:109M cs:Z:*tccM:1*tcgN:1*cagE:14*gacE:1*cagE*ccaV:35*ttgI:2*gacE:18*atgL:5*gtcL:1*gtgI:3*ttgM*acgV*accQ*cagT:1*gctG*ttgI*caaK:9 +CM113344.1:1105885-1164023 miniprot mRNA 24284 24613 493 - . ID=BC000001;Rank=1;Identity=0.8349;Positive=0.9450;Target=MH260578_SUI1 1 109 +CM113344.1:1105885-1164023 miniprot CDS 24284 24613 493 - 0 Parent=BC000001;Rank=1;Identity=0.8349;Target=MH260578_SUI1 1 109 +CM113344.1:1105885-1164023 miniprot stop_codon 24284 24286 0 - 0 Parent=BC000001;Rank=1 +##PAF MH260578_SLA2 1072 29 1072 + CM113344.1:1105885-1164023 58139 25003 28162 1719 3186 0 AS:i:2782 ms:i:2782 np:i:766 fs:i:0 st:i:0 da:i:21 do:i:0 cg:Z:98M3D69M1D91M8D8M2I37M2I258M4I53M4D129M1I236M3D55M cs:Z:*tccT:1*ttgI*cagS:1*tcgA*gttI*cgcK:2*actC*tcaT*gcaP*gatE*gagD:2*ccgS:19*tccD:2*aacE*gctT*cttM:1*ctaV*ctcV:1*attL*caaK:1*gacT*gagD*gttT:7*actL*gctI:3*attL:4*gatP:3*gccV*gatG:1*tacF*agaK:1*ttgM*aacE:2*gatS*ggcN:1*ggaD*agaT*tctL*accR*ttcT*caaL*ggtD*gatR*ggcR:1*aagQ-ggttatggc*aggV:2*aggP:3*actN:1*ttgI*attL*agaQ:2*ctgR:2*agaH:17*gtcR:2*caaS:2*gatN:3*gaaD:3*gaaD:1*atgT:7*gatS*ttaF*caaG:1*ttgD:3*gcaT*attV-tct*catR*gacN*aagN*agaN:3*aagI:6*ctcI:10*atcL*accI:5*gttM:1*cgtK*tcaM*actS*ggtD:1*gacA*gatE*gccI:5*caaS*cgcK*tatF*gatE*tctD:2*gctE:2*tttY*gagD:3*gacN:2*tcgN:5*tcaT:5*agaK:2*caaF*tctE:4*ttgI:1*aagQ*gatN:1-gataataactctcctccacaacca:3*gagN*atcV*caaN*agaL:1+PL*gaaT*tccE*cgtQ*atgK:1*agtA*caaA*tcaT:1*aacL*cacR*attH*gagS:1*gagT:1*atcF*tcaE*gaaQ*caaE*ctaN*accE*atgR*tctQ:1*ttcQ:1*cagA:3*gttT:4*gttM+VR*gatN*tttA*tggF*aacV*aatE:2*accQ*gatL:2*actQ*gagQ:1*attQ*cgaQ:1*caaE:1*gagQ:1*gaaQ:1*caaE*actL*ctaQ:4*tatQ*gctQ*gaaL*caaA:1*agaQ*ctcQ*ttgY*tttW:4*gtgR:2*caaA:1*caaA:1*cgtQ:3*gagQ*aatQ:1*attL*cgtL:2*tacA:1*tcgR*caaH:11*ctaI:1*gccT:1*agaK:3*gaaD*caaN:3*ttgM:2*caaS:1*tacD:1*cgtK*attV*aagQ:3*actN:1*attL:1*accS:1*gctT:1*tctT:1*actQ*caaE:2*ggaA:4*ttgQ*attL*caaN*aatL:1*catQ:2*atcV*aatE*ttgY:16*cagT:6*aacT:1*tttS:2*ttgI:11*gatE:6*aaaR:1*ctcM:1*tcaA:2*gtcI:3*ggaD:2*cgtK:7*gacE:2*aagR*ttgF:1*ggtN:1*aaaT*ggtK*gatT*gaaD*attL*gatQ*atgT:1*gaaQ:1*caaE*attK*agaD*gacE:1*actE*ttgQ:2*gatA*gatT*tctL*gaaD:1*aacA:2*tccA:2*tccT*actA:2*gctN:2*aagN:2*attL*actQ*aatE:1*gaaQ*tcaN*caaK*ttaF*agaK+DVTL*caaN*caaA*caaP:1*gctD*tctE*tttK*aacL*ccaK*gacQ:2*caaE*ctaQ:1*aagQ*gatE:1*gaaD*gagL:1*ttgI:1*attM*gttM:5*gatE*gctT*ctaI*gctK*gaaD:1*gccV:1*gctT:1*cagN*gagS*aatR*gatE*tccE*gccK*attS:1*gaaM:1*attN*gatK*caaL-gtcctagttgat*catN*ttaS:2*ttaV*accL*tcgG:1*gttI:4*ctaK:2*cttI*aagN:1*cttI:2*tccA*attV*tatF:10*caaL:5*ttcY*ttaV:1*tccT*atcL*attL:4*acaG*tctL*gcaV:4*aacS:3*gatN*tttM*atcL*gctV:2*ccaD:1*ggtA*gatN*catE*tctA*gaaL*atcV:1*cgtD:1*ttgI*aacT*gatN:2*tctT:4*gtgI:3*tccT:2*attL*gttT:2*gttT:1*acaQ:1*tccD*gagF*gctQ:1*cagD*atcL:1*tccD*tgtT:1*agaK*tccD*actI:2*gaaI:2*gagV*tttY*tttL*aaaE:2*cttY+H:1*gatN*atgL*ttgV*gaaN*ggtK*gatS*gaaI*gaaD*gcaD*aaaQ*atgT*gaaD*gttI:2*gttN:2*ttgI*agtD:3*aaaI:2*gaaT:1*accL:2*gctV:1*gatS*ttgM*gctK*ccaT*aaaP:1*aacS*cttK*ttgI*aacD*aatL*aacS*agaK*ggtV*gctD*gctG*aacE:3*attL:2*aatK:1*ttaM*caaE*aatH:1*gctS*caaR*tctV:1*gctE*acgE:2*ttgV*caaH:1*aatS:3*accN*aaaQ*aagP*caaF:1*ccaV:1*atcM:1*caaS:2*tttI:1*attV:2*gcaS:5*attS:1*gttI*acaI*aatT*tcaA:1*gagK*attY:2*aagR:1*gcaS:1*gctE:6*gctN*aagN*aacG*aagR:2*attH:3*gagS*tatF:3*catN:1*cgtK:8*gctS:2*gttI:1*cttY:2*aaaN*gttT:2*gaaR*actI:5*tcaQ*ggtN*acaK*aaaH*acaS*catN:3*atcV:3*aatR:6*gttA:3*gcaS:1*tcaA:3*gcaS*accQ:1*ttaM:4*gatN*aacK:2*gctD:3*agtK:1*actN*tctL:3*ggcQ:2*aatD:2*cagN*gatK*atcL*ttgI*actI*agaG*agaK*agtH*aatE-ggtggtggt*gaaV*aatE:1*ttaI:6*aatI:5*gttA:15*attS*gcaS:10*tatF*ggaS:1*agaK:1*ggtD:4*ggtE +CM113344.1:1105885-1164023 miniprot mRNA 25004 28165 2782 + . ID=BC000002;Rank=1;Identity=0.5395;Positive=0.7213;Target=MH260578_SLA2 30 1072 +CM113344.1:1105885-1164023 miniprot CDS 25004 28165 2782 + 0 Parent=BC000002;Rank=1;Identity=0.5395;Target=MH260578_SLA2 30 1072 +CM113344.1:1105885-1164023 miniprot stop_codon 28163 28165 0 + 0 Parent=BC000002;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 195 46 195 - CM113344.1:1105885-1164023 58139 29132 29585 150 489 0 AS:i:135 ms:i:117 np:i:74 fs:i:0 st:i:0 da:i:159 do:i:54 cg:Z:15M3I3M6I11M2D5M7D21M1I18M1D36M2I22M4D6M cs:Z::5*aagT*cttT:1*acaP*aaaL*tgcL*cagK*aacR*tttL*attL+TEY*gcaG*agcA*ctgI+DNGKDS:3*aagV*aagA*aagP:2*aatS:1*aaaR-caaatg:1*acaR:1*ccgQ:1-aaattaaacaagcccaaacaa*aaaE*cgcK*acgK*aaaR:4*attM:1*tacF:3*tttY:4*tccA*acaN+Y*gagN*cacS:1*ggcK*gacA:2*ttaQ*ataD:2*aagD*caaA:1*gagN*agtR*agtE*cctK-gga*tttH:1*ttgQ:2*gctS*cgcL:2*gctI*gagQ:2*ttaH*ttcS*ttgK:1*cagK*cacE:1*gaaF*atgS*acgR*tttW*gttL*gacE*tggM*attK:1*cttS*tcgN*acaK*tcaL*gagG*gcaK*ttaF+NS*cagD*gaaD*gctP*gtgT*aatT:1*tcgR*cttE*catI*actN*acaV*ccaM*gatS*cccH*tcaI*cgaV*attV*cagE:1*attV:1*aacQ-ccaggggagggc*aggE*agcC:4 +CM113344.1:1105885-1164023 miniprot mRNA 29133 29585 117 - . ID=BC000003;Rank=1;Identity=0.3067;Positive=0.4540;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 47 195 +CM113344.1:1105885-1164023 miniprot CDS 29133 29585 117 - 0 Parent=BC000003;Rank=1;Identity=0.3067;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 47 195 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATa1 181 122 181 - CM113344.1:1105885-1164023 58139 28251 28428 99 177 0 AS:i:172 ms:i:172 np:i:45 fs:i:0 st:i:0 da:i:108 do:i:9 cg:Z:59M cs:Z::4*accR*ccaH*atgI*tctP*aagE*gcaS*acaS*aggK*gcaE:3*gaaK*gtcA:1*gaaK*atgV*attK*aagR*acaF:2*cgaS:3*gcgE*gcgR:2*gaaR*aaaE:2*atgI*acgS:12*atgA:3*ggaS*gtaR:1 +CM113344.1:1105885-1164023 miniprot mRNA 28252 28428 172 - . ID=BC000004;Rank=1;Identity=0.5593;Positive=0.7627;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +CM113344.1:1105885-1164023 miniprot CDS 28252 28428 172 - 0 Parent=BC000004;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 123 181 +##PAF 5476_sc5314_MTL_A_MTLA1 210 121 197 - CM113344.1:1105885-1164023 58139 28260 28488 105 228 0 AS:i:175 ms:i:175 np:i:49 fs:i:0 st:i:0 da:i:48 do:i:18 cg:Z:76M cs:Z:*gaaD*gagD*gaaD*aacK*tccD:1*ggaE*tcaD*ccaD*agcI*actL*accI*gaaK*aacG*gtgE*aacE:1*tcaK*gaaK*acgS:4*accQ*ccaR*atgL*tctD*aagN*gcaS:1*aggK*gcaE*ttaF:2*gaaK:3*atgK*attN:1*acaQ:3*aaaR:2*gcgE*gcgL:4*tgtH:1*atgV*acgS*cctL*ttaS:1*gtaI:10*tcaK:1 +CM113344.1:1105885-1164023 miniprot mRNA 28261 28488 175 - . ID=BC000005;Rank=1;Identity=0.4605;Positive=0.6447;Target=5476_sc5314_MTL_A_MTLA1 122 197 +CM113344.1:1105885-1164023 miniprot CDS 28261 28488 175 - 0 Parent=BC000005;Rank=1;Identity=0.4605;Target=5476_sc5314_MTL_A_MTLA1 122 197 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1019 7 1019 + CM113344.1:1105885-1164023 58139 24997 28153 1518 3177 0 AS:i:2430 ms:i:2430 np:i:720 fs:i:0 st:i:0 da:i:15 do:i:9 cg:Z:98M1D191M19D7M2I272M7D21M4D7M7D70M1I34M1D24M1I32M3I15M5D178M3D56M cs:Z::1*gagN*tccL:1*ttgI:1*accA:1*gttL*cgcK:2*actC*tcaT:2*gagA*actA:20*aaaR*tccE:2*aacH*gctC:7*aacS*gacN*gagD*gttT:1*ttgI:2*gccT:2*actV*gctI:3*attL:4*gatP*tccT*gctC:1*gccI*gatG:2*agaK:1*ttgI*aacS:1*attL*gatE*ggcS:1*ggaS:1*tctF*accS*ttcN*caaN:1*gatT*ggcA-ttc*aagA:2*ggcT:3*aggK:3*actF:2*attE*agaQ:2*ctgK:2*agaH*aacD:6*acaM:9*gtcR:2*caaS:2*gatN:2*tatF:1*gccS:1*ttgM*gaaD:1*atgL:7*gatN:3*ttgV:2*gccS*gcaS:1*tctR:1*gacT*aagS*agaE:3*aagV:2*gcaS:3*ctcI*gttI:9*atcL*accI:3*agaK:1*gttL:1*cgtK:1*actS*ggtE:2*gatE*gccV*ttgI*gaaA:3*caaD:1*tatF:1*tctV:2*gctR:12*cgtK:3*tcaT:1*atcV:6*caaS:1*gctP:1*aacI:1*ttgQ*atcD*aagE*gatE:1*gatP*aatQ*aacG*tctF:1*ccaL*caaV*ccaR:1*gacR*gagS*gagS*atcV*caaS*agaN*ccaS:1*tccE*cgtS*atgN*actI*agtP*caaR:1*actS:1-cacattgagcctgagcctatctcagaacaactaaccatgtctcaattccaacagcca:1*gctQ*gttS:4+AM:2*tttS*tggF*aacV*aatR:2*accQ*gatE*tacL:1*actE*gagQ:1*attR*cgaQ*ttgA*caaE*caaE*gagR:1*gaaQ:1*caaE*actL:1*caaE:2*cagM*tatL*gctQ*gaaR*caaD:1*agaA*ctcR*ttgF*tttW:1*caaE:2*gtgR:2*caaA*gagV*caaS:1*cgtE:3*gagS*aatQ:1*attL*cgtA:2*tacM:1*tcgR:4*cgtK*gtcL:1*gaaQ:2*cgtQ:3*gccT:1*agaG:2*tatH:1*caaR:3*ttgM:1*cagE:2*tacD:2*attV:1*gcaV:2*actN:1*attL*gctR*accI*actV*gctT:1*tctT:1*actE*caaE:2*ggaA*tctV:1*gatT*gaaS*ttgQ*attC*caaS*aatH:1*catE:2*atcV*aatQ*ttgY:23*aacG:3*aaaI*ttgV:9*gctS:12*tcaA:2*gtcI:3*ggaD:2*cgtK:9*gaaA*aagR*ttgI:2*ggtN*aaaD*ggtS*gatE*gaaQ*attV*gatQ:2*gaaR*ttaT:1*attL:2*ttgM*actE*ttgT:2*gatK*gatS*tctL:1*agaE*aacT*caaH:1*tccQ:2*tccA*actS*atcL:1*gctS:2*aagS:3*actE*aatE*ttgI*gaaK*tcaA*caaS*ttaA*agaS*caaE*caaL*caaK*tctE*gctG*tctG*tttL:1*ccaE*gacE*cttA*gaaQ:1-ctacttaaggataaggaagag:1*ttgV*gagQ*attM*gttL:2*acaA:3*gctT*ctaM*gctQ:3*aagE*gctQ*caaH:1-gagaatgattcc:3*gaaK*cagE*attA*gatE-caagtcctagttgatcattta:1*aaaR*ttaV*accL*tcgR:1*gttI:1*gctT*atcL:1*ctaK*agtV*ggcS*cttS*aagD:2*caaR*gatE*tccF*attV*tatF*gaaN:1*gatE:2*atgV*caaY*gcaS*ggaN*aatT*caaD*aacI*tctP*tctT*ccaA:1*ttcY:1*cttG*tccT*atcM*attL:1*aagR*tcaV:1*acaH*tctL*gcaS:1*gacQ:1*gcaS*aacT*tctT*ttcM*aacT:1*tttY:3*ggaN*ccaM*aatK:1*gatE+S*catE:1*gaaG*atcL:1*cgtK*tcgA*ttgI*aacI*gatE*ttcL:1*tctD:2*tcaG*gatN*gtgL*ttgF*ttgS:1*tccV:1*ggtA*attV*gttL:1*ttgD*gttR:1*acaD:1*tccV*gagQ-gct*gatE*cagI*atcL*attV*tccE*tgtG*gctS*agaK*tccD*actL:1*gaaH*gaaM*tctV:1*gagL:2*aaaE:1*ttaM:2*gatN+H*atgL:1*gaaK*ggtL*gatE*gaaI*gaaH*gcaE*aaaR*atgT*gaaD*gttR:2*gttT:2*ttgI*agtN:3*aaaV*cttI:1*gaaS:1*accM:1*ctgV*gctV:1+VFR:1*ttgP*gctG*ccaR:1*tccT*aacP*cttV*ttgS*aacS*aatD*aacE*agaL:1*gctY-gctaacttgagtgac*attF*gttL:1*aatK:2*caaA:1*gcaT*gctQ*caaA*tctA*atcV*gctE*acgK:1*tctA*ttgG*caaL:1*aatE:1*ttaM*ctcS:1*aaaR*aagH*caaK*gacL*ccaE*tcaV*atcQ*tctE*caaD*attG*gacS*tttL:1*attV:1*aagE:2*ttaI*gctS*gctC:1*attS:1*gttI*acaI:1*tcaA*attV*gagK*attL:1*atcL*aagE:1*gcaC:1*gctD*tcaA:5*gctS*aagR*aacG*aagR:2*attM:1*aaaR*actA*gagL*tatF:3*catN:10*gctS:1*tctQ:2*cttY*tccA:1*aaaG*gttI:2*gaaR*actM:5*tcaA:2*aaaN*acaS*catS:11*gcaS*tccA:1*gttA:3*gcaS*gctS:4*gcaS*accD*tttL*ttaM:1*aagQ*actS*caaH*gatL:3*gctE:2*aaaR*agtK:3*gcaS:2*ggcM:2*aatT*caaK:1*cagR*gatS*atcL:1*actL-agaagaagt*aatS*ggtD*ggtE*ggtS*gaaN*aatK*gatT*ttaV:3*aagQ:1*tctT*aatA:3*aagR:1*gttA:1*atgL:10*aatS:2*attS*gcaH:10*tatF*ggaS:3*ggtE*gacE:1 +CM113344.1:1105885-1164023 miniprot mRNA 24998 28153 2430 + . ID=BC000006;Rank=1;Identity=0.4778;Positive=0.6799;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +CM113344.1:1105885-1164023 miniprot CDS 24998 28153 2430 + 0 Parent=BC000006;Rank=1;Identity=0.4778;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 187 128 187 - CM113344.1:1105885-1164023 58139 28251 28428 99 177 0 AS:i:172 ms:i:172 np:i:45 fs:i:0 st:i:0 da:i:108 do:i:9 cg:Z:59M cs:Z::4*accR*ccaH*atgI*tctP*aagE*gcaS*acaS*aggK*gcaE:3*gaaK*gtcA:1*gaaK*atgV*attK*aagR*acaF:2*cgaS:3*gcgE*gcgR:2*gaaR*aaaE:2*atgI*acgS:12*atgA:3*ggaS*gtaR:1 +CM113344.1:1105885-1164023 miniprot mRNA 28252 28428 172 - . ID=BC000007;Rank=1;Identity=0.5593;Positive=0.7627;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +CM113344.1:1105885-1164023 miniprot CDS 28252 28428 172 - 0 Parent=BC000007;Rank=1;Identity=0.5593;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 129 187 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 190 43 173 - CM113344.1:1105885-1164023 58139 29168 29585 120 420 0 AS:i:137 ms:i:137 np:i:72 fs:i:0 st:i:0 da:i:159 do:i:90 cg:Z:64M1I18M1D20M3D15M6D12M cs:Z::5*aagT*cttT:1*acaP*aaaL*tgcL*cagK*aacR*tttL*attL*gcaS*agcE*ctgY*ctgG*aatA*aagI*aagD*aagN*aagG*aagN*aagD*aatS:1*aaaN*caaK*atgV*cacA*acaP*gtgK*ccgK*aagS*aaaR*ttaR*aacH*aagR*cccV*aaaQ*caaK*aaaQ*cgcK*acgK*aaaR:4*attM:1*tacF:3*tttY:4*tccA*acaN+Y*gagN*cacS:1*ggcK*gacA:2*ttaQ*ataD:2*aagD*caaA:1*gagN*agtR*agtE*cctK-gga*tttH:1*ttgQ:2*gctS*cgcL:2*gctI*gagQ:2*ttaH*ttcS*ttgK:1*cagK*cacE:1-gaaatgacg:1*gttS*gacI:1*attL*ttaE*cttM*tcgK*acaL*tcaN*gagK*gcaK:1*cagR*gaaK-gctgtgaatagatcgctt*catN*actN*acaS*ccaD:2*tcaT*cgaT*attR*cagR*gatE:1 +CM113344.1:1105885-1164023 miniprot mRNA 29169 29585 137 - . ID=BC000008;Rank=1;Identity=0.2857;Positive=0.5143;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 44 173 +CM113344.1:1105885-1164023 miniprot CDS 29169 29585 137 - 0 Parent=BC000008;Rank=1;Identity=0.2857;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 44 173 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 294 0 294 + CM113344.1:1105885-1164023 58139 34152 35031 498 885 0 AS:i:842 ms:i:835 np:i:213 fs:i:0 st:i:0 da:i:12 do:i:0 cg:Z:107M1I90M1I44M1D51M cs:Z:*acaM*accN*acaS*actD*cctK*gtcS:1*gccK*gcaS*tcaI*aaaA:1*actS*caaA*aaaN*tggF:6*tttA*ggtA*ggtS*gccM*gttF:2*tgcL:15*cctK*cttV:1*aaaG*ccaD:2*ttcV*cagS*atgL:1*cttF*tccK:1*cttI:1*caaT*gatE:1*attV*cccL*ggtA*atgA:1*tctA:2*agtT:1*ggtS:4*ggtA:3*ctcT*acaA:6*tatK:2*gggE*aatI*ttcM*gtcT*cctD*gcaP*gacT*aagR*acaG*aagQ*tcaA*atgS*gctT+F*tatQ:2*cctA*atcA:2*tttI*agtA:5*atcV:4*tccA:1*attV:8*acgN*ggtS:2*cttE*gatS*aagQ:2*aatH:1*gcgK:2*tttL:2*gttL*attL*agaK:1*gttT*aagR*gatE:1*ggtN*atgL*aatT:6*tctG*actP:2*gtcA:2*gttI:3*tctA:4*acgS:2*ttgV:2*gaaK:2*atcV+E*actN:1*ggaS:1*aaaD*ggaP*gatK*tctT*ttcK*ttcA:1*tttH:1*aacS*tctA:2*cttI:7*atcV:13*gcaS:1*tcaG*gcaT:1-aat:2*gtcS*attF*gaaG:2*acaK*actD:2*aagS*aatR:2*ccaL*agtG:6*gttT:1*agtA:1*gtaI:4*aacH:2*attL:2*ttgI:1*gtgL:1*caaE:1*aagR*catR:2*gtgI:2 +CM113344.1:1105885-1164023 miniprot mRNA 34153 35034 835 + . ID=BC000009;Rank=1;Identity=0.5627;Positive=0.7220;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +CM113344.1:1105885-1164023 miniprot CDS 34153 35034 835 + 0 Parent=BC000009;Rank=1;Identity=0.5627;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +CM113344.1:1105885-1164023 miniprot stop_codon 35032 35034 0 + 0 Parent=BC000009;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 305 25 305 + CM113344.1:1105885-1164023 58139 34197 35031 492 843 0 AS:i:834 ms:i:834 np:i:211 fs:i:0 st:i:0 da:i:57 do:i:0 cg:Z:89M2I93M1I39M1D56M cs:Z:*tggF:6*tttA*ggtA*ggtS*gccM*gttF:2*tgcL:15*cctS*cttQ:1*aaaG*ccaQ*tccN:1*ttcL*cagT*atgI:1*cttI*tccR:1*cttI*aaaS*caaS*gatE:2*cccL:1*atgA:6*ggtS:4*ggtA:3*ctcT:4*gtcI:2*tatA:2*gggD*aatY*ttcL*gtcT*cctK:1*gacN*aagN*acaN*aagQ+DL:1*atgT:1*tatI:3*atcC:2*tttL*agtA:1*gccG*gttI:2*atcL:6*attV:8*acgS*ggtS:2*cttK*gatP*aagA:4*gcgR*catN:1*tttL*gatH:1*gttL*attA:1*atcM*gttA:1*gatE:2*atgL*aatG*gcaS*cttW:4*tctL*actP:2*gtcT:6*tctA:4*acgS:2*ttgV:2*gaaK*cttF:1*atcV+K*actK:1*ggaS*atgF*aaaD*ggaE*gatT*tctT*ttcR*ttcA:1*tttH:1*aacS*tctA:2*cttM:7*atcV:6*ctcV:6-gca:1*tcaA*gcaE*ggaK*aatH:2*gtcI:1*gaaT*atcV:4*atcM*aagR*aatK:3*agtG:1*atgL:4*gttF:3*gtaI:4*aacH:2*attL:2*ttgV:1*gtgL:3*aagR*catT*ttaW*aagR*gtgI*ggtA*cttM +CM113344.1:1105885-1164023 miniprot mRNA 34198 35034 834 + . ID=BC000010;Rank=1;Identity=0.5836;Positive=0.7509;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 26 305 +CM113344.1:1105885-1164023 miniprot CDS 34198 35034 834 + 0 Parent=BC000010;Rank=1;Identity=0.5836;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 26 305 +CM113344.1:1105885-1164023 miniprot stop_codon 35032 35034 0 + 0 Parent=BC000010;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1045 0 1045 + CM113344.1:1105885-1164023 58139 24988 28153 1815 3198 0 AS:i:2897 ms:i:2897 np:i:787 fs:i:0 st:i:0 da:i:6 do:i:9 cg:Z:94M1D122M1I62M3D17M10D9M1I5M3I258M1I11M3I178M1I40M3D31M1I3M2D151M2D53M cs:Z:*aaaM*accS*atcR*tctQ:1*tccA:2*cagK*accV:1*gttI*cgcK:2*actC:1*gcaT*gatE:1*actA:12*tacF:4*aaaR:3*tccA:1*tggY:1*gctG:1*aaaR:8*gttI*cagP:3*gccS:3*gctI:3*attL:4*gatP:2*ttgI*gccK*gatE:1*tacI*agaK:1*ttgR*aacD:2*gatQ*ggcS:2*agaH-tct*accV:1*caaP:3*ttcM:1*ggtR:8*gtcD*actR:5*ttaI*ctgD:2*agaN*aacS:1*agaK:14*gtcK:2*caaS:2*gatN:6*ttgM*gaaD:2*tcaV:4*ttgI*gatN:5*atcL:2*gcaS:1*tctD*catS*gacS*aagS*agaH:2*tgtL:8*gttI:6*tacF:8*gccG*gttM*tatC*cgtH:1*actL:1*tccH:2+E:2*gaaQ:2*aagI*caaE:1*tatF*gatI:2*cacY*gctS*cgtD:1*tttY*gagD:3*gacE:2*tcgY*attV*cgtK:5*atcV*accN:2*agaK:2*caaF*tctE:2*aacD:1*ttgF*atcN*aagW:1*gatE*gatT*aatD*aacE*tctL*cctK:1*caaR*ccaI*gagL*gacA:2-atccaaaga:1*gaaS*tccL*cgtQ*atgV*actS:1*caaK*tcaA*actP*aacS*cacE*attE*gagF*cctG*gagT:1-atctcagaacaactaaccatgtctcaattc*caaT*cagE*ccaV:1*gctH*gttS:3+A:1*gttT:1*tttL:1+AQS:1*aatA*caaL*caaK*accQ*gatQ:2*actQ:2*attQ*cgaL:1*caaE:3*gaaQ*gctQ:1*actL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*ctcE*ttgY*tttW:1*caaK:1*caaK*gtgE*caaL:1*caaE*gagQ:2*cgtL*ctcY:2*gagQ*aatQ:1*attE*cgtS:2*tacL:1*tcgR:1*gctV:4*gctG:7*gccI:4*tatF*gaaD*caaR:3*ttgM:1*cagR:2*tacD*cagE:1*attV:6*attL*gctS*accS:1*gctT:1*tctN:1*actQ:4*tctA:1*gatE:2*attL*caaG:1*ctaI*catQ:2*atcA*aatA*ttgQ:2*aatS:2*gagD:10*cagE:6*aacS:3*aaaT*ttgA:1*caaL:2*gctS:2*caaR:8*ttgI:1*aaaR:3*tcaA:2*gtcI:3*ggaD:13*aagR*ttgS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gatE*atgR:2*ttaA*caaR*attL:1*gacE*ttgQ*actE*ttgT:2*gatA:1*tctI:1*agaK*aacL*caaH:2*aatE:1*tccK*actS*atcF*tttL*gctS:2*aagN:3*actE*aatE:1*gaaQ*tcaN:2*agaQ*caaA*caaS*caaS+L:1*gctE:1*tttA:1*ccaE*gacR:1*gaaK*caaA:1+EAQ:1*aagQ*gatE:1*gaaD*gagD:2*gagA:1*gttA*caaH:3*gatE*gatA*gctT*ctaI*gctK:2*gccT*aagG*gctQ:1*cagS*gagI*aatQ*gatN:3*gagD:4*caaA*gtcI:1*gttL*gatE:1*ttaI*accD:3*tcgE*ttgV*gttI:4*ctaT:2*cttV*aagQ:1*cttI:2*tccG*attL*tatF:16*ttcY:1*cttS:7*acaN*tctL:2*gacE:1*gcaT*aacS:3*gatG:10*tctV*gaaS*atcV:1*cgtQ*tcgT*ttgI*aacT*gatE:2*tctT:2*tcaV:1*gtgL:3*tccC:2*attV:3*gttA*aagS*acaS:1*tccQ:2*gatE*cagA*atcL*attL*tccN*tgtS:2*tccD*actV:5*gagS:1*tttI*aaaV:2*cttY:1*gatS*atgS:1*gaaK:1+L*gatS*gaaD*gaaQ*gcaE:1*atgT*gaaD*gttK*gttA:1*gttE:2*ttgV*agtD*gttI:1*gaaQ*aaaE:2*gaaT:1*accS*caaS:1*gctV:1*gatK:1*gctI*ccaS*aaaS*tccQ*aacV*cttN*ttgI:1*aatV:1-agaggtgct*gctD:1*ttgV*agtG*gacE*attL:2*aatR:1*ttaM*caaA*aatH:2*caaE*tctT:2*acgK:2*ttgD*caaH:1*aatT*tctD:3*aaaN+A:3-ccatca:2*caaT:2*tttM*gaaK:2*aagN*gcaS:2*gctS:6*aatK*tcaA:1*gagI*attL:3*gctT:1*attT*gctD*tcaC:1*gagN:3*gctN*aagH*aacG*aagR:2*attS:1*aaaR:1*gagS*tatF:5*cgtK:8*gctS:4*cttY:2*aaaN*gttS:2*gaaQ*actI:6*ggtS:1*aaaS*acaS*catP:1*gaaQ:4*tccC:1*gaaD:5*gttA:10*accS:1*ttaM:5*aacK:2*gctL:9*aaaR*ggcS:2*aatR:3*gatE:2*actS*agaQ*agaK*agtD*aatK-ggtggt*ggtE:1*aatL*gatE*ttaI:6*aatA:6*gaaQ:11*aatT:2*attQ*gcaN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE*agtE +CM113344.1:1105885-1164023 miniprot mRNA 24989 28153 2897 + . ID=BC000011;Rank=1;Identity=0.5675;Positive=0.7383;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +CM113344.1:1105885-1164023 miniprot CDS 24989 28153 2897 + 0 Parent=BC000011;Rank=1;Identity=0.5675;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1044 0 1044 + CM113344.1:1105885-1164023 58139 24988 28150 1815 3195 0 AS:i:2897 ms:i:2897 np:i:787 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:94M1D122M1I62M3D17M10D9M1I5M3I258M1I11M3I178M1I40M3D31M1I3M2D151M2D52M cs:Z:*aaaM*accS*atcR*tctQ:1*tccA:2*cagK*accV:1*gttI*cgcK:2*actC:1*gcaT*gatE:1*actA:12*tacF:4*aaaR:3*tccA:1*tggY:1*gctG:1*aaaR:8*gttI*cagP:3*gccS:3*gctI:3*attL:4*gatP:2*ttgI*gccK*gatE:1*tacI*agaK:1*ttgR*aacD:2*gatQ*ggcS:2*agaH-tct*accV:1*caaP:3*ttcM:1*ggtR:8*gtcD*actR:5*ttaI*ctgD:2*agaN*aacS:1*agaK:14*gtcK:2*caaS:2*gatN:6*ttgM*gaaD:2*tcaV:4*ttgI*gatN:5*atcL:2*gcaS:1*tctD*catS*gacS*aagS*agaH:2*tgtL:8*gttI:6*tacF:8*gccG*gttM*tatC*cgtH:1*actL:1*tccH:2+E:2*gaaQ:2*aagI*caaE:1*tatF*gatI:2*cacY*gctS*cgtD:1*tttY*gagD:3*gacE:2*tcgY*attV*cgtK:5*atcV*accN:2*agaK:2*caaF*tctE:2*aacD:1*ttgF*atcN*aagW:1*gatE*gatT*aatD*aacE*tctL*cctK:1*caaR*ccaI*gagL*gacA:2-atccaaaga:1*gaaS*tccL*cgtQ*atgV*actS:1*caaK*tcaA*actP*aacS*cacE*attE*gagF*cctG*gagT:1-atctcagaacaactaaccatgtctcaattc*caaT*cagE*ccaV:1*gctH*gttS:3+A:1*gttT:1*tttL:1+AQS:1*aatA*caaL*caaK*accQ*gatQ:2*actQ:2*attQ*cgaL:1*caaE:3*gaaQ*gctQ:1*actL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*ctcE*ttgY*tttW:1*caaK:1*caaK*gtgE*caaL:1*caaE*gagQ:2*cgtL*ctcY:2*gagQ*aatQ:1*attE*cgtS:2*tacL:1*tcgR:1*gctV:4*gctG:7*gccI:4*tatF*gaaD*caaR:3*ttgM:1*cagR:2*tacD*cagE:1*attV:6*attL*gctS*accS:1*gctT:1*tctN:1*actQ:4*tctA:1*gatE:2*attL*caaG:1*ctaI*catQ:2*atcA*aatA*ttgQ:2*aatS:2*gagD:10*cagE:6*aacS:3*aaaT*ttgA:1*caaL:2*gctS:2*caaR:8*ttgI:1*aaaR:3*tcaA:2*gtcI:3*ggaD:13*aagR*ttgS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gatE*atgR:2*ttaA*caaR*attL:1*gacE*ttgQ*actE*ttgT:2*gatA:1*tctI:1*agaK*aacL*caaH:2*aatE:1*tccK*actS*atcF*tttL*gctS:2*aagN:3*actE*aatE:1*gaaQ*tcaN:2*agaQ*caaA*caaS*caaS+L:1*gctE:1*tttA:1*ccaE*gacR:1*gaaK*caaA:1+EAQ:1*aagQ*gatE:1*gaaD*gagD:2*gagA:1*gttA*caaH:3*gatE*gatA*gctT*ctaI*gctK:2*gccT*aagG*gctQ:1*cagS*gagI*aatQ*gatN:3*gagD:4*caaA*gtcI:1*gttL*gatE:1*ttaI*accD:3*tcgE*ttgV*gttI:4*ctaT:2*cttV*aagQ:1*cttI:2*tccG*attL*tatF:16*ttcY:1*cttS:7*acaN*tctL:2*gacE:1*gcaT*aacS:3*gatG:10*tctV*gaaS*atcV:1*cgtQ*tcgT*ttgI*aacT*gatE:2*tctT:2*tcaV:1*gtgL:3*tccC:2*attV:3*gttA*aagS*acaS:1*tccQ:2*gatE*cagA*atcL*attL*tccN*tgtS:2*tccD*actV:5*gagS:1*tttI*aaaV:2*cttY:1*gatS*atgS:1*gaaK:1+L*gatS*gaaD*gaaQ*gcaE:1*atgT*gaaD*gttK*gttA:1*gttE:2*ttgV*agtD*gttI:1*gaaQ*aaaE:2*gaaT:1*accS*caaS:1*gctV:1*gatK:1*gctI*ccaS*aaaS*tccQ*aacV*cttN*ttgI:1*aatV:1-agaggtgct*gctD:1*ttgV*agtG*gacE*attL:2*aatR:1*ttaM*caaA*aatH:2*caaE*tctT:2*acgK:2*ttgD*caaH:1*aatT*tctD:3*aaaN+A:3-ccatca:2*caaT:2*tttM*gaaK:2*aagN*gcaS:2*gctS:6*aatK*tcaA:1*gagI*attL:3*gctT:1*attT*gctD*tcaC:1*gagN:3*gctN*aagH*aacG*aagR:2*attS:1*aaaR:1*gagS*tatF:5*cgtK:8*gctS:4*cttY:2*aaaN*gttS:2*gaaQ*actI:6*ggtS:1*aaaS*acaS*catP:1*gaaQ:4*tccC:1*gaaD:5*gttA:10*accS:1*ttaM:5*aacK:2*gctL:9*aaaR*ggcS:2*aatR:3*gatE:2*actS*agaQ*agaK*agtD*aatK-ggtggt*ggtE:1*aatL*gatE*ttaI:6*aatA:6*gaaQ:11*aatT:2*attQ*gcaN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +CM113344.1:1105885-1164023 miniprot mRNA 24989 28150 2897 + . ID=BC000012;Rank=1;Identity=0.5681;Positive=0.7390;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +CM113344.1:1105885-1164023 miniprot CDS 24989 28150 2897 + 0 Parent=BC000012;Rank=1;Identity=0.5681;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 305 25 305 + CM113344.1:1105885-1164023 58139 34197 35031 492 843 0 AS:i:834 ms:i:834 np:i:211 fs:i:0 st:i:0 da:i:57 do:i:0 cg:Z:89M2I93M1I39M1D56M cs:Z:*tggF:6*tttA*ggtA*ggtS*gccM*gttF:2*tgcL:15*cctS*cttQ:1*aaaG*ccaQ*tccN:1*ttcL*cagT*atgI:1*cttI*tccR:1*cttI*aaaS*caaS*gatE:2*cccL:1*atgA:6*ggtS:4*ggtA:3*ctcT:4*gtcI:2*tatA:2*gggD*aatY*ttcL*gtcT*cctK:1*gacN*aagN*acaN*aagQ+DL:1*atgT:1*tatI:3*atcC:2*tttL*agtA:1*gccG*gttI:2*atcL:6*attV:8*acgS*ggtS:2*cttK*gatP*aagA:4*gcgR*catN:1*tttL*gatH:1*gttL*attA:1*atcM*gttA:1*gatE:2*atgL*aatG*gcaS*cttW:4*tctL*actP:2*gtcT:6*tctA:4*acgS:2*ttgV:2*gaaK*cttF:1*atcV+K*actK:1*ggaS*atgF*aaaD*ggaE*gatT*tctT*ttcR*ttcA:1*tttH:1*aacS*tctA:2*cttM:7*atcV:6*ctcV:6-gca:1*tcaA*gcaE*ggaK*aatH:2*gtcI:1*gaaT*atcV:4*atcM*aagR*aatK:3*agtG:1*atgL:4*gttF:3*gtaI:4*aacH:2*attL:2*ttgV:1*gtgL:3*aagR*catT*ttaW*aagR*gtgI*ggtA*cttM +CM113344.1:1105885-1164023 miniprot mRNA 34198 35034 834 + . ID=BC000013;Rank=1;Identity=0.5836;Positive=0.7509;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 26 305 +CM113344.1:1105885-1164023 miniprot CDS 34198 35034 834 + 0 Parent=BC000013;Rank=1;Identity=0.5836;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 26 305 +CM113344.1:1105885-1164023 miniprot stop_codon 35032 35034 0 + 0 Parent=BC000013;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 305 25 305 + CM113344.1:1105885-1164023 58139 34197 35031 492 843 0 AS:i:834 ms:i:834 np:i:211 fs:i:0 st:i:0 da:i:57 do:i:0 cg:Z:89M2I93M1I39M1D56M cs:Z:*tggF:6*tttA*ggtA*ggtS*gccM*gttF:2*tgcL:15*cctS*cttQ:1*aaaG*ccaQ*tccN:1*ttcL*cagT*atgI:1*cttI*tccR:1*cttI*aaaS*caaS*gatE:2*cccL:1*atgA:6*ggtS:4*ggtA:3*ctcT:4*gtcI:2*tatA:2*gggD*aatY*ttcL*gtcT*cctK:1*gacN*aagN*acaN*aagQ+DL:1*atgT:1*tatI:3*atcC:2*tttL*agtA:1*gccG*gttI:2*atcL:6*attV:8*acgS*ggtS:2*cttK*gatP*aagA:4*gcgR*catN:1*tttL*gatH:1*gttL*attA:1*atcM*gttA:1*gatE:2*atgL*aatG*gcaS*cttW:4*tctL*actP:2*gtcT:6*tctA:4*acgS:2*ttgV:2*gaaK*cttF:1*atcV+K*actK:1*ggaS*atgF*aaaD*ggaE*gatT*tctT*ttcR*ttcA:1*tttH:1*aacS*tctA:2*cttM:7*atcV:6*ctcV:6-gca:1*tcaA*gcaE*ggaK*aatH:2*gtcI:1*gaaT*atcV:4*atcM*aagR*aatK:3*agtG:1*atgL:4*gttF:3*gtaI:4*aacH:2*attL:2*ttgV:1*gtgL:3*aagR*catT*ttaW*aagR*gtgI*ggtA*cttM +CM113344.1:1105885-1164023 miniprot mRNA 34198 35034 834 + . ID=BC000014;Rank=1;Identity=0.5836;Positive=0.7509;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 26 305 +CM113344.1:1105885-1164023 miniprot CDS 34198 35034 834 + 0 Parent=BC000014;Rank=1;Identity=0.5836;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 26 305 +CM113344.1:1105885-1164023 miniprot stop_codon 35032 35034 0 + 0 Parent=BC000014;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1044 0 1044 + CM113344.1:1105885-1164023 58139 24988 28150 1815 3195 0 AS:i:2897 ms:i:2897 np:i:787 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:94M1D122M1I62M3D17M10D9M1I5M3I258M1I11M3I178M1I40M3D31M1I3M2D151M2D52M cs:Z:*aaaM*accS*atcR*tctQ:1*tccA:2*cagK*accV:1*gttI*cgcK:2*actC:1*gcaT*gatE:1*actA:12*tacF:4*aaaR:3*tccA:1*tggY:1*gctG:1*aaaR:8*gttI*cagP:3*gccS:3*gctI:3*attL:4*gatP:2*ttgI*gccK*gatE:1*tacI*agaK:1*ttgR*aacD:2*gatQ*ggcS:2*agaH-tct*accV:1*caaP:3*ttcM:1*ggtR:8*gtcD*actR:5*ttaI*ctgD:2*agaN*aacS:1*agaK:14*gtcK:2*caaS:2*gatN:6*ttgM*gaaD:2*tcaV:4*ttgI*gatN:5*atcL:2*gcaS:1*tctD*catS*gacS*aagS*agaH:2*tgtL:8*gttI:6*tacF:8*gccG*gttM*tatC*cgtH:1*actL:1*tccH:2+E:2*gaaQ:2*aagI*caaE:1*tatF*gatI:2*cacY*gctS*cgtD:1*tttY*gagD:3*gacE:2*tcgY*attV*cgtK:5*atcV*accN:2*agaK:2*caaF*tctE:2*aacD:1*ttgF*atcN*aagW:1*gatE*gatT*aatD*aacE*tctL*cctK:1*caaR*ccaI*gagL*gacA:2-atccaaaga:1*gaaS*tccL*cgtQ*atgV*actS:1*caaK*tcaA*actP*aacS*cacE*attE*gagF*cctG*gagT:1-atctcagaacaactaaccatgtctcaattc*caaT*cagE*ccaV:1*gctH*gttS:3+A:1*gttT:1*tttL:1+AQS:1*aatA*caaL*caaK*accQ*gatQ:2*actQ:2*attQ*cgaL:1*caaE:3*gaaQ*gctQ:1*actL:1*caaE:2*cagH*tatQ*gctQ*gaaQ:2*agaL*ctcE*ttgY*tttW:1*caaK:1*caaK*gtgE*caaL:1*caaE*gagQ:2*cgtL*ctcY:2*gagQ*aatQ:1*attE*cgtS:2*tacL:1*tcgR:1*gctV:4*gctG:7*gccI:4*tatF*gaaD*caaR:3*ttgM:1*cagR:2*tacD*cagE:1*attV:6*attL*gctS*accS:1*gctT:1*tctN:1*actQ:4*tctA:1*gatE:2*attL*caaG:1*ctaI*catQ:2*atcA*aatA*ttgQ:2*aatS:2*gagD:10*cagE:6*aacS:3*aaaT*ttgA:1*caaL:2*gctS:2*caaR:8*ttgI:1*aaaR:3*tcaA:2*gtcI:3*ggaD:13*aagR*ttgS:1*ggtS*ggtD:1*ggtN*gatS:1*attT*gatE*atgR:2*ttaA*caaR*attL:1*gacE*ttgQ*actE*ttgT:2*gatA:1*tctI:1*agaK*aacL*caaH:2*aatE:1*tccK*actS*atcF*tttL*gctS:2*aagN:3*actE*aatE:1*gaaQ*tcaN:2*agaQ*caaA*caaS*caaS+L:1*gctE:1*tttA:1*ccaE*gacR:1*gaaK*caaA:1+EAQ:1*aagQ*gatE:1*gaaD*gagD:2*gagA:1*gttA*caaH:3*gatE*gatA*gctT*ctaI*gctK:2*gccT*aagG*gctQ:1*cagS*gagI*aatQ*gatN:3*gagD:4*caaA*gtcI:1*gttL*gatE:1*ttaI*accD:3*tcgE*ttgV*gttI:4*ctaT:2*cttV*aagQ:1*cttI:2*tccG*attL*tatF:16*ttcY:1*cttS:7*acaN*tctL:2*gacE:1*gcaT*aacS:3*gatG:10*tctV*gaaS*atcV:1*cgtQ*tcgT*ttgI*aacT*gatE:2*tctT:2*tcaV:1*gtgL:3*tccC:2*attV:3*gttA*aagS*acaS:1*tccQ:2*gatE*cagA*atcL*attL*tccN*tgtS:2*tccD*actV:5*gagS:1*tttI*aaaV:2*cttY:1*gatS*atgS:1*gaaK:1+L*gatS*gaaD*gaaQ*gcaE:1*atgT*gaaD*gttK*gttA:1*gttE:2*ttgV*agtD*gttI:1*gaaQ*aaaE:2*gaaT:1*accS*caaS:1*gctV:1*gatK:1*gctI*ccaS*aaaS*tccQ*aacV*cttN*ttgI:1*aatV:1-agaggtgct*gctD:1*ttgV*agtG*gacE*attL:2*aatR:1*ttaM*caaA*aatH:2*caaE*tctT:2*acgK:2*ttgD*caaH:1*aatT*tctD:3*aaaN+A:3-ccatca:2*caaT:2*tttM*gaaK:2*aagN*gcaS:2*gctS:6*aatK*tcaA:1*gagI*attL:3*gctT:1*attT*gctD*tcaC:1*gagN:3*gctN*aagH*aacG*aagR:2*attS:1*aaaR:1*gagS*tatF:5*cgtK:8*gctS:4*cttY:2*aaaN*gttS:2*gaaQ*actI:6*ggtS:1*aaaS*acaS*catP:1*gaaQ:4*tccC:1*gaaD:5*gttA:10*accS:1*ttaM:5*aacK:2*gctL:9*aaaR*ggcS:2*aatR:3*gatE:2*actS*agaQ*agaK*agtD*aatK-ggtggt*ggtE:1*aatL*gatE*ttaI:6*aatA:6*gaaQ:11*aatT:2*attQ*gcaN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +CM113344.1:1105885-1164023 miniprot mRNA 24989 28150 2897 + . ID=BC000015;Rank=1;Identity=0.5681;Positive=0.7390;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +CM113344.1:1105885-1164023 miniprot CDS 24989 28150 2897 + 0 Parent=BC000015;Rank=1;Identity=0.5681;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 287 14 287 + CM113344.1:1105885-1164023 58139 34215 35031 486 822 0 AS:i:810 ms:i:803 np:i:203 fs:i:0 st:i:0 da:i:75 do:i:0 cg:Z:86M1I90M1I44M1D51M cs:Z::1*tttA*ggtA*ggtS*gccM*gttF:2*tgcL:15*cctK*cttV:1*aaaG*ccaD:2*ttcV*cagS*atgL:1*cttY*tccK:1*cttV:1*caaT*gatE:1*attV*cccL*ggtA*atgA:1*tctA:4*ggtS:4*ggtA:3*ctcT*acaA:6*tatK:3*aatI*ttcM*gtcT*cctD*gcaP*gacT:1*acaG*aagQ*tcaA*atgS*gctT+F*tatQ:2*cctA*atcA:2*tttI*agtA:5*atcV:4*tccA:1*attV:8*acgN*ggtS:2*cttE*gatS*aagQ:2*aatH:1*gcgK:2*tttL:2*gttL*attL*agaK:1*gttT*aagR*gatE:1*ggtN*atgI*aatT:6*tctG*actP:2*gtcA:2*gttI:3*tctA:4*acgS:2*ttgV:2*gaaK:2*atcV+E*actN:1*ggaS:1*aaaD*ggaP*gatK*tctT*ttcK*ttcA:1*tttH:1*aacS*tctA:2*cttI:7*atcV:13*gcaS:1*tcaG*gcaT:1-aat:2*gtcS*attF*gaaG:2*acaK*actD:2*aagS*aatR:2*ccaL*agtG:6*gttT:1*agtA:1*gtaI:4*aacH:2*attL:2*ttgI:1*gtgL:1*caaE:1*aagR*catR:2*gtgI:2 +CM113344.1:1105885-1164023 miniprot mRNA 34216 35034 803 + . ID=BC000016;Rank=1;Identity=0.5912;Positive=0.7409;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +CM113344.1:1105885-1164023 miniprot CDS 34216 35034 803 + 0 Parent=BC000016;Rank=1;Identity=0.5912;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 15 287 +CM113344.1:1105885-1164023 miniprot stop_codon 35032 35034 0 + 0 Parent=BC000016;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 195 46 195 - CM113344.1:1105885-1164023 58139 29132 29585 150 489 0 AS:i:135 ms:i:117 np:i:74 fs:i:0 st:i:0 da:i:159 do:i:54 cg:Z:15M3I3M6I11M2D5M7D21M1I18M1D36M2I22M4D6M cs:Z::5*aagT*cttT:1*acaP*aaaL*tgcL*cagK*aacR*tttL*attL+TEY*gcaG*agcA*ctgI+DNGKDS:3*aagV*aagA*aagP:2*aatS:1*aaaR-caaatg:1*acaR:1*ccgQ:1-aaattaaacaagcccaaacaa*aaaE*cgcK*acgK*aaaR:4*attM:1*tacF:3*tttY:4*tccA*acaN+Y*gagN*cacS:1*ggcK*gacA:2*ttaQ*ataD:2*aagD*caaA:1*gagN*agtR*agtE*cctK-gga*tttH:1*ttgQ:2*gctS*cgcL:2*gctI*gagQ:2*ttaH*ttcS*ttgK:1*cagK*cacE:1*gaaF*atgS*acgR*tttW*gttL*gacE*tggM*attK:1*cttS*tcgN*acaK*tcaL*gagG*gcaK*ttaF+NS*cagD*gaaD*gctP*gtgT*aatT:1*tcgR*cttE*catI*actN*acaV*ccaM*gatS*cccH*tcaI*cgaV*attV*cagE:1*attV:1*aacQ-ccaggggagggc*aggE*agcC:4 +CM113344.1:1105885-1164023 miniprot mRNA 29133 29585 117 - . ID=BC000017;Rank=1;Identity=0.3067;Positive=0.4540;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 47 195 +CM113344.1:1105885-1164023 miniprot CDS 29133 29585 117 - 0 Parent=BC000017;Rank=1;Identity=0.3067;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT 47 195 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 181 122 181 - CM113344.1:1105885-1164023 58139 28251 28428 99 177 0 AS:i:172 ms:i:172 np:i:45 fs:i:0 st:i:0 da:i:108 do:i:9 cg:Z:59M cs:Z::4*accR*ccaH*atgI*tctP*aagE*gcaS*acaS*aggK*gcaE:3*gaaK*gtcA:1*gaaK*atgV*attK*aagR*acaF:2*cgaS:3*gcgE*gcgR:2*gaaR*aaaE:2*atgI*acgS:12*atgA:3*ggaS*gtaR:1 +CM113344.1:1105885-1164023 miniprot mRNA 28252 28428 172 - . ID=BC000018;Rank=1;Identity=0.5593;Positive=0.7627;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +CM113344.1:1105885-1164023 miniprot CDS 28252 28428 172 - 0 Parent=BC000018;Rank=1;Identity=0.5593;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 123 181 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 440 0 440 + CM113344.1:1105885-1164023 58139 26788 28153 585 1383 0 AS:i:840 ms:i:840 np:i:302 fs:i:0 st:i:0 da:i:195 do:i:9 cg:Z:19M4D7M7D70M1I34M1D24M1I32M3I15M5D175M1I7M4D51M cs:Z:*gagQ*attM*gttL:2*acaA:3*gctT*ctaM*gctQ:3*aagE*gctQ*caaH:1-gagaatgattcc:3*gaaK*cagE*attA*gatE-caagtcctagttgatcattta:1*aaaR*ttaV*accL*tcgR:1*gttI:1*gctT*atcL:1*ctaK*agtV*ggcS*cttT*aagD:2*caaR*gatE*tccF*attV*tatF*gaaN:1*gatE:2*atgV*caaY*gcaS*ggaN*aatT*caaD*aacI*tctP*tctT*ccaA:1*ttcY:1*cttG*tccT*atcM*attL:2*tcaV:1*acaH*tctL*gcaS:1*gacQ:1*gcaS*aacT*tctT*ttcM*aacT:1*tttY:3*ggaN*ccaM*aatR:2+S*catE:1*gaaG*atcL:1*cgtK*tcgA*ttgI*aacT*gatE:2*tctD:2*tcaG*gatN*gtgL*ttgF*ttgS:1*tccV:1*ggtA*attV*gttL:1*ttgD*gttR:1*acaD:1*tccV*gagQ-gct*gatE*cagI*atcL*attV*tccE*tgtG:1*agaK*tccD*actL:1*gaaH*gaaM*tctV:1*gagL:2*aaaE:1*ttaM:3+H*atgL:1*gaaK*ggtL*gatE*gaaI*gaaH*gcaE*aaaR*atgT*gaaD*gttR:2*gttT:2*ttgI*agtN:3*aaaV*cttI:1*gaaS:1*accM:1*ctgV*gctV:1+VFR:1*ttgP*gctG*ccaR:2*aacP*cttV*ttgS*aacS*aatD*aacE*agaL:1*gctY-gctaacttgagtgac*attF*gttL:1*aatK:2*caaA:1*gcaT*gctQ*caaA*tctA*atcV*gctE*acgK:1*tctA*ttgD*caaL:1*aatE:1*ttaM*ctcS:1*aaaR*aagH*caaK*gacL*ccaE*tcaV*atcQ*tctE*caaG*attG*gacS*tttL:1*attV:1*aagE:2*ttaI*gctS*gctC:1*attS:1*gttI*acaI:1*tcaA*attV*gagK*attL:1*atcL*aagE:1*gcaC:1*gctE*tcaA:5*gctS*aagR*aacG:3*attL:1*aaaR*actA*gagL*tatF:3*catN:10*gctS:1*tctQ:2*cttY*tccA:1*aaaG*gttI:2*gaaR*actM:5*tcaA:2*aaaN*acaS*catS:11*gcaS*tccA:1*gttA:3*gcaS*gctS:4*gcaS*accD*tttL*ttaM:1*aagQ*actS*caaH*gatL:3*gctE:2*aaaR*agtK:3*gcaS:2*ggcM:2*aatT*caaK:1*cagR*gatS+L*atcL:2*agaD*agaE:2-ggtggtggtgaa*aatK*gatT*ttaV:3*aagQ:1*tctT*aatA:3*aagR:1*gttA:1*atgL:10*aatS:2*attS*gcaQ:10*tatF*ggaS:3*ggtE*gacE:1 +CM113344.1:1105885-1164023 miniprot mRNA 26789 28153 840 + . ID=BC000019;Rank=1;Identity=0.4230;Positive=0.6551;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +CM113344.1:1105885-1164023 miniprot CDS 26789 28153 840 + 0 Parent=BC000019;Rank=1;Identity=0.4230;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 671 0 671 + CM113344.1:1105885-1164023 58139 24988 28162 858 2208 0 AS:i:1085 ms:i:1112 np:i:404 fs:i:0 st:i:0 da:i:6 do:i:0 cg:Z:94M9I9M4I76M6I2M2I87M28I7M2I8M1I14M2I38M6I19M3D8M1I21M2I39M22D45M7D12M1D15M321U11M4D14M21D14M7D15M1I2M3I25M873U1M7I19M cs:Z:*aaaM*accS*atcR*tctE:1*tccQ*gacT:1*cagE*accK:1*gttI*cgcK:2*actL:1*gcaG*gatG*gagD*actV:21*tccT:1*tggF:1*gctL:3*ctcQ:1*attY*caaA*aacT:2*gttL:6*atcV*actL*gctL:3*attL:4*gatP:3*gccV*gatE:1*tacI:2*ttgI*aacD:2*gatE*ggcS:1*ggaS:1+IFSVDSDDG:1*accK*ttcY*caaG*ggtS*gatS*ggcR*ttcM*aagR+YIGD*ggtE:1*ggcA*aggK*ttgA:1*aggG:3*actF*tacF:1*attV*agaE:2*ctgQ:2*agaK*aacS:1*agaK:4*acaI:10*acgS:1*caaS:2*gatN:4*gccT*attV:1*gaaD:2*tcaN:1*caaE:1*tctV*ttgA:1*gatN:2*agaK:4*gcaG:2*catN*gacR:1+SGFGSF*agaS:1+GL*gagD:1*aagR*atcG*tctA*gcaV:1*gtgI:3*gcaS:1*tctT:3*tacF:2*atcV:3*ttgM*agaK:1*gttL:1*cgtK*tcaQ*actM:1*tccD*gacY:3*gaaS*cctG:1*aagF*caaD:2*gatT*tctE:1*cacC:3*tttN:3*gctF:2*tccN*tcgT*attV*cgtQ*tacP:1*accK*tcaN:5*agaT:2*caaS*tctD*gctP*ccaM*aacS*ttgR*ttgI:1*aagD:1*gatE:1+DAEVNDVVFEKRSKSSVSSLPSRTPTRE:1*aacL*tctR*cctD:1*caaS:1+VH:1*gacA*gagP*gagP*atcV*caaA:2+A:1*tccV:1*atgS*actL:2*tcaP*actA*aacA*cacP*attV*gagV:1+IA*gagT*cctG*atcV*tcaV*gaaM*caaP*ctaV*accV*atgT*tctG*caaY*ttcS:1*cagP:1*attL*gctQ:1*caaV:3*gttA*gatE:2*aacS*aatI*caaP*caaS:1*gatS*tacA*gaaP:1*gagR*caaS*attV+VASHDD*cgaL:1*caaI*caaE*gagQ*cgtQ*gaaK*gctQ:1*actQ*ctaI:6*gaaL:1-cagagactc:1*tttE*gagH:1*cagK:1*gtgL:1+L:5*cgtA:1*gctL:1*gagQ*aatR*ctcI*attQ*cgtQ*gatQ*cagE*tacM*caaE*tcgK*caaE:1+LI:1*ggtQ*cgtK*gtcT:1*gaaS:1*gaaQ*cgtN:1*ctaI*ttgM*gccS*ttgF*agaK*ggtN:3*caaR:2*ttgV*ttgM:1*cagE:2*tacD*cagK:1*attV*aagQ*gcaK:2*actS:1*attL-gctaccactgctcaatctgccactcaacaattgggatctaaggatgaattgattcaaaatctacat:1*cagS*atcV*aatK*ttgE*tggF:1*aatE:2*gagQ:15*ctaI:1*aacK:1*tttM:2*ttgT:1*caaD*aagD*gctL:1*tctE:1*caaE*gaaS*gctE*attV-gataaacgtgaaaaattggaa:1*gatK:3*aagS*aatA*gtcL*gaaS*ttgN*gcaV:1-ttg:2*gaaN*cgtN*gatN*cgtK*gccG*cgtE*ttgS*gacE*ttgR:1*aagE*ttgA:1*gD~gt318ag-tt*gatR*catS*ttaG*accM*aaaI:1*accK:3*gatE-gctatcttacta:1*ggcS*cttI:1*agaS:3*tccP:1*tatA*gaaS:2-tctccaatgcaagcaggaaatcaaaactcttctccagaattcttactttccatcattgaaaag*tcaV:1*acaD*tctL:3*tttL*gcaS:1*tctA:1*aacI:1-tttatcgctgatggaccaaat:2*catI*tctN*gaaT:1*attV*cgtN*tcgI:1*aacP*gatE:1*actV:1+L*tctL*attL+RCP*tcaQ*gatE*gtgM*ttgQ*ttgT*aacI*tccA*aaaE*ggtD*attF*gttL:1*ttgC*gttL*aagS*acaS*gatA*tccK*gagQ*gctT:1*cagS*atcV:1*tccN*tA~gt870ag-ca*agaN+IAFQEKL:7*aagQ:1*ggaS:2*gatY:5*ggtV +CM113344.1:1105885-1164023 miniprot mRNA 24989 28165 1112 + . ID=BC000020;Rank=1;Identity=0.3886;Positive=0.5489;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 671 +CM113344.1:1105885-1164023 miniprot CDS 24989 26570 1025 + 0 Parent=BC000020;Rank=1;Identity=0.4112;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 557 +CM113344.1:1105885-1164023 miniprot CDS 26889 27230 30 + 2 Parent=BC000020;Rank=1;Identity=0.2373;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 558 643 +CM113344.1:1105885-1164023 miniprot CDS 28101 28165 57 + 2 Parent=BC000020;Rank=1;Identity=0.5556;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 644 671 +CM113344.1:1105885-1164023 miniprot stop_codon 28163 28165 0 + 0 Parent=BC000020;Rank=1 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 228 109 211 - CM113344.1:1105885-1164023 58139 28260 28533 108 306 0 AS:i:154 ms:i:154 np:i:56 fs:i:0 st:i:0 da:i:3 do:i:18 cg:Z:37M11I54M cs:Z:*tctN:2*ataD*cttV*tctK*acaD*aagE*gctS:1*cccM*agaS*acaF*tttD*aacT:1*gagH:1*aacC*tccT*agtD*ggaT:1*ccaE*agcD*actI*accS*gaaD*aacK*gtgS*aacE:2*gaaS:1*aagN*aaaS+DNPIQNCYPTY:2*accS*ccaF*atgI*tctK*aagH*gcaE*acaS:1*gcaG*ttaI:2*gaaK*gtcI:1*gaaK*atgV*attK*aagQ*acaC:2*cgaT*aaaS:2*gcgL*gcgY:2*gaaQ:1*tgtL*ggcD*atgL:2*ttaS:3*gttI:8*tcaA:1 +CM113344.1:1105885-1164023 miniprot mRNA 28261 28533 154 - . ID=BC000021;Rank=1;Identity=0.3529;Positive=0.5490;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 110 211 +CM113344.1:1105885-1164023 miniprot CDS 28261 28533 154 - 0 Parent=BC000021;Rank=1;Identity=0.3529;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 110 211 +##PAF 4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 126 65 126 - CM113344.1:1105885-1164023 58139 28260 28428 99 183 0 AS:i:165 ms:i:165 np:i:45 fs:i:0 st:i:0 da:i:108 do:i:18 cg:Z:11M5I45M cs:Z::2*aagE*agaK*accS:1*atgK*tctG:1*gcaS*acaS+ISPQA:2*ttaF:2*gaaQ:2*gaaR*atgR*attK*aagQ*acaS*ccaL:1*cgaS:2*cgaK*gcgE*gcgE*attV:1*gaaK:3*atgI:9*acaI:7 +CM113344.1:1105885-1164023 miniprot mRNA 28261 28428 165 - . ID=BC000022;Rank=1;Identity=0.5410;Positive=0.7377;Target=4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 66 126 +CM113344.1:1105885-1164023 miniprot CDS 28261 28428 165 - 0 Parent=BC000022;Rank=1;Identity=0.5410;Target=4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 66 126 +##PAF 1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1064 0 1064 + CM113344.1:1105885-1164023 58139 24973 28159 1782 3234 0 AS:i:2873 ms:i:2873 np:i:784 fs:i:0 st:i:0 da:i:-1 do:i:3 cg:Z:6M2D97M2D169M4I47M7D261M8I23M3I159M1I41M2D189M1D56M cs:Z:*caaM*ggcA*gctT*atgL:2-accatc*tctA:1*tccA*gacE:1*cagT*accV*tcgH*gttI*cgcK:3*tcaN*gcaP*gatE:10*gccQ:2*gtcI:4*catN*aaaH:1*tccG*aaaR*tccA:3*gctG*cttM:1*ctaI*ctcQ:1*attL:6*ttgV:6*gctI:3*attL:4*gatP:1*gctV:1*gccR*gatE*ggaA*tacQ*agaQ:1*ttgR*aacS:1*attL*gatE*ggcS:1*ggaA:1*tctG*accA*ttcH*caaA:1*gatR-ggcttc*aagH*ggtT:1*ggcA*aggK:2*aggY:3*actG*tacL:1*attL*agaK:2*ctgN:2*agaK:2*agaP*ggaD:10*gtcI:2*gtcR*acgN*gttI*caaD:2*gatN:3*gaaQ:2*ttgV*gaaD:2*tcaD:3*tctA*ttgI:1*gatN*ttaF:4*tttI:1*gcaS*attL*tctK:1*gacG*aagR*agaP*tctN:2*aagR:4*gtgI:10*aagR:1*atcT:1*tccT:4*gttM*tatH*cgtA*tcaT:1*ggtN*tccD:7*aagR*caaS:2*gatY:3*gctQ:2*tttL:3*gctY:4*attL*cgtK:4*ttgV:4*agaT:1*ccaS:1*tctD*gctP:3*ttgY*atcG*aagE*gatN*gatP*gatE*aatE*aacG*tctE+APAL:1*ccaK*caaR:1*gagI*gacS:1*gagV*atcA*caaS*agaL*ccaA*gaaT*tccG*cgtA*atgT:1*agtA*caaP:2*aacT*cacD*attL*gagT:3*atcV*tcaV*gaaD*caaF*ctaW*accS*atgT*tctP:1*ttcG:2*ccaQ*attL*gctQ*gttQ:1*ccaQ*acaL-ggtgttgatttttggaacaat:2*accQ*gatQ:2*actE:2*attR:2*caaA*caaA*gagQ:3*caaE*actL*ctaQ:3*cagM*tatL*gctH*gaaL:2*agaQ*ctcQ*ttgQ:2*caaE:2*gtgR*caaM:1*caaA:8*aatA:1*attL:5*tcgR:2*cagD:2*gtcA:6*ctaI:1*gccN:6*caaR:3*ttgM:1*cagE:2*tacD*cagR:1*attV:1*gcaG:2*actG:1*attL*gctQ*accQ*actV*gctI*caaA*tctN*gccT*actN*caaL:1*ttgS*ggaQ:3*gaaD*ttgM:1*caaR*aatS:1*catQ:2*atcV:1*ttgS*tggL:1*aatS:2*gagD*agtV:13*aatD*ctaK:1*aacK*aagE*tttL:1*aaaL:1*caaK*caaV:9*gatE:6*aaaR:1*ctcI:1*tcaA:2*gtcI:3*ggaD:9*ttgY:2*gaaD:1*ttgS*aagR:1*ggtN*aaaQ*ggtK:1*gaaV:1*gatE*atgK:2*ttaR:1*attL:1*gacL*ttgT*actE*ttgD:2*gatA:1*tctA:2*aacS*caaK*tcaG*tccA*aatD:2*actL*atcL*tttI*gctS*cagK:1*aagN*aagR:1*attL*actA*aatD:2*tcaE*caaA:1*agaK*caaA*caaK:2*gctV*tctL*tttD:1+YANRSFDD*ccaS:2*gaaK*caaL*ctaK:1*aagA*gatE:1*gaaD*gagD:4*gttL:1*caaE:1*atgV:1*gatS+MEA:2*gctK:1*ctaM*gccS*aagL*gctN*caaH*cagN:1*aatA:1*tccQ:1*attL:5*caaE:2*gttK*gatE:2*accS:2*accN:1*ttgI*gttI:1*gctS*atcV:1*ctaE:1*ggcS*cttA*aagE:1*cttV:1*gatE:1*attL*tatF:1*ttgF*gatE:10*tctT:2*ttcY:4*attV:2*tcaA:8*aacT:3*gatN:2*gctI:6*catY:1*gaaG:2*cgtS*tcgT*ttgA*aacT*gatI*ttcL*actS:1*tctV:1*tcaP:3*ttgN:1*tccA:2*attL*gttT:2*gttA*aagR*acaD:1*tccT*gagV:2*cagN:3*tgtY:2*tccL*actA:1*gaaV*gaaA*tctV:1*gagD:2*aaaS*tcaG*ttaV*cttQ:1*gatN*atgK:1+A*gaaQ*ggtL*gatS*gaaD:1*gcaD:1*atgT*gaaD*gttK:2*gttN*ggtF:1*ttgI*agtD:2*gaaT*aaaH:2*gaaS:1*accS:1*ctgV*gctS:1*gatK:1*gctT:1*aaaR*tccA*aacG*cttT*ttgH*aacL:1-aacaga:1*gctS*gctG*aacD:1*agtG:1*attL:2*aatR:1*ttaM*caaA*aatR:2*caaE*tctA:1*gctE*acgS:1*tctN*ttgA*caaK:2*tctE:2*accS*aaaR*aagP*caaK:2*tcaK*atcF:1*caaV*attF:1*tttL*gaaK:1*aacH*aagE:10*aatT*tcaA:1*gagA*attA:5*attS*gctD*tcaC:1*gagQ:4*aagQ*aacG:2*tctA*attS:1*aaaR:1*gagA*tatF:19*cttA:2*aaaN:12*acaN*aaaH:1*catP:1*gaaQ:9*gcaS:2*gttA:10*accS*tttY*ttaM:5*aacR:2*gctG:3*agtA:5*aaaR*ggcS:9*actS*agaK*agaK*agtQ*aatQ-ggt*ggtK*ggtE:3*ttaV:5*tctT*aatP:1*gaaV*aatL:2*gttT:13*gcaS:1*attN*gcaS:11*ggaS:1*agaV:1*ggtD*gacE*agtD*gacE:1 +CM113344.1:1105885-1164023 miniprot mRNA 24974 28159 2873 + . ID=BC000023;Rank=1;Identity=0.5510;Positive=0.7273;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +CM113344.1:1105885-1164023 miniprot CDS 24974 28159 2873 + 0 Parent=BC000023;Rank=1;Identity=0.5510;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +##PAF 4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1053 0 1046 + CM113344.1:1105885-1164023 58139 24982 28159 1647 3216 0 AS:i:2605 ms:i:2596 np:i:755 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:94M2I3M1I177M8D5M3D30M1D5M6I32M2D257M2I12M7D136M1I36M4D36M1I153M1D57M cs:Z::2*aaaS*accN*atcR*tctA:1*tccV:2*cagN*accV*tcgN*gttI*cgcK:3*tcaN*gcaT:2*actS:14*tggH:2*aaaR:3*tccA:3*gctG*cttI*aaaR*ctaI*ctcQ:1*attL:1*aacT:1*gagD:1*cagM*ttgV:6*gctI:3*attL:4*gatP:3*gccR:1*ggaA*tacQ*agaA:1*ttgV:1*tggF:1*gatQ*ggcS:1+AR:1*agaN*tctN+Y:1*ttcG*caaP*ggtN*gatQ:1*ttcN*aagH:3*aggK:2*aggS:3*actR*tacF:1*attM*agaQ:2*ctgT:2*agaK*aacY:1*agaP*ggaA:3*acaM:4*gagD:1*gtcI:2*gtcR:2*caaN:2*gatN:6*ttgM*gaaN:2*tcaT:3*tctG*ttgV:2*ttaF:3*atcV:1*gccS*gcaS*attL*tctQ*catR*gacS*aagR*agaS*tctN:5*gcaS:3*ctcM*gttI:7*aagR:1*atcV*accI:5*gttM*tatH*cgtV:3*tccN:1*gatE:3*cctS:1*aagR:2*tatF*gatY:3*gctD:2*tttR*gagD:2*gctY:3*tcgT*attL*cgtK:2*accR:1*ttgI*atcV:3*agaQ:1*ccaG*caaK*tctN*gctP:4*atcE*aagE*gatE:1*gatG*aatP*aacG*tctL:1-ccacaaccagaggacgaggagatc:3*gaaK:1-cgtatgact*agtV*caaA*tcaE:1*aacR*cacS*attV*gagS:3*atcA*tcaP*gaaL*caaP*ctaV*accA*atgT*tctP*caaT*ttcP*caaS*cagI:1*attA*gctE*gttA:2*acaI-ggt*gttE*gatN:2*aacS+QDALLA*aatQ:2*accY:1*tacA:1*actQ:1*caaR*attL:1*ttgQ:2*gagA*cgtM:1*gctE*caaE*actR*ctaI*caaR:1*caaM:1*tatM*gctQ*gaaQ:2*agaQ-ctcttg:2*caaM:2*gtgR:2*caaM:1*caaA:1*cgtQ*ctcR:3*aatQ:1*attM*cgtA:2*tacM*caaA*tcgR*caaH:4*gtcM:6*ctaI:7*gaaD:4*ttgM:1*cagE:2*tacD*cagG:1*attV:4*actA:1*attL*gctN*accQ*actL*gctQ:1*tctT:1*actH:1*caaS*ttgA*ggaQ*tctA:2*gaaD:2*caaE*aatS:1*catQ*gaaQ:2*aatT*ttgM:1*aagR*aatQ:3*agtT:3*ttaR:2*caaS*ttaM:1*cagE:1*catY:1*aatA:2*aacK:1*tttL:1*aaaA*ttgT:7*caaK:3*gatE:1*cgtA:4*aaaR:1*ctcM*aaaR*tcaH:2*gtcI:3*ggaD:2*cgtK:6*ttgY:2*gaaD*aagR*ttgA:3*aaaN*ggtK*gatE*gaaD*attV*gatE*atgR:2*ttaR*caaE*attL:1*gacM*ttgA*actQ*ttgD:2*gatA:1*tctK*gaaD:1*aacS*caaT*tcaG*tccA*aatD:2*actL*atcL*tttL*gctS*cagK:1*aagN*aagR:1*attL*actS*aatE:2*tcaN*caaA:1*agaK*caaM*caaK:1*tctR:1*tctL*tttD*aacE*ccaR*gacG*cttD*gaaD*caaS*ctaD:1*aagL*gatR*aagR*gaaL:2*ttgK:2*gttE*caaY*caaE*acaA*atgL*gatN*gatE:1*ctaF*gctN*gaaS:2+LH*aagQ*gctE:2*gagG:1*gatN:1*gccN*attG*gagG*gaaS-cagattgatcaagtcctagtt*gatT*catP:1*accA*aaaA:1*accH:1*ttgI*gttI:2*atcL:1*ctaE:2*cttS*aagQ:1*cttV:2*tccA*attL*tatF:2*gatE:10*tctT:2*ttcY:3*atcV:3*tcaA:1*acaE:2*actS*gacA:2*aacT:3*gatN:1*atcL:2*ggaE*ccaT*aatD:2*catY*tctA:3*cgtK*tcgT*ttgV:1*gatI*ttcY*actS*tctT*tctA*attV*tcaE*gatN:2*ttgS:4*attL*gttT:2*gttA:1*acaD:1*tccA*gagS:2*cagA*atcL*attV*tccN*tgtF:2*tccE*actS:2*gaaA*tctT:1*gagR*tttN:1*aaaI*tcaG:3*gatE*atgI*ttgI:1*ggtD+F*gatP*gaaL*gaaD*gcaD:3*gttR:2*gttT*ggtL:1*ttgM*agtN:2*gaaT*aaaA:2*gaaH:2*caaK:3*gatR*ttgM:3*tccT:1*cttI-ttgaacaataac*agaN*ggtM*gctS*gctG*aacD:1*agtG:1*attL:1*gatE*aatR:1*ttaM*caaA*aatK:2*caaD*tctA:2*acgA:2*ttgA*caaK:1*aatG*tctD:2*accK*aaaF*aagN:1+S:2*tcaL*atcK:1*caaT*attT:1*tttL*gaaQ*attL*aacH*aagE:1*atcA*ttaI*gctQ:2*attQ:2*acaI:1*tcaA:1*gagA*attA:2*aagR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aagR:1*tctT*attS:1*aaaR*actA*gagQ*tatF:3*catN:1*cgtK:13*cttA:2*aaaN*gttI:3*actK:3*gttT:1*tcaR*ggtR:1*aaaS*acaG*catL:8*gaaN:5*gttA:12*ttaM:5*aacK:2*gctE*gcaC:2*agtV:5*aaaR*ggcN:2*aatK:3*gatE:2*actN*agaK*agaK*agtF-aat:1*ggtE*ggtL*gaaD*aatE*gatK*ttaV:2*tcaA*aagA:2*aatK:2*aatF:2*gttT:13*gcaD:1*attQ*gcaG:6*gagQ*atcM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gacE:1 +CM113344.1:1105885-1164023 miniprot mRNA 24983 28159 2596 + . ID=BC000024;Rank=1;Identity=0.5121;Positive=0.7043;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +CM113344.1:1105885-1164023 miniprot CDS 24983 28159 2596 + 0 Parent=BC000024;Rank=1;Identity=0.5121;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +##PAF 4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1054 0 1047 + CM113344.1:1105885-1164023 58139 24982 28159 1644 3219 0 AS:i:2591 ms:i:2582 np:i:755 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:94M2I3M1I177M8D5M3D30M1D5M6I32M2D257M2I12M7D136M1I36M4D21M1I15M1I153M1D57M cs:Z::2*aaaS*accN*atcR*tctA:1*tccV:2*cagN*accV*tcgN*gttI*cgcK:3*tcaN*gcaT:2*actS:14*tggH:2*aaaR:3*tccA:3*gctG*cttI*aaaR*ctaI*ctcQ:1*attL:1*aacT:1*gagD:1*cagM*ttgV:6*gctI:3*attL:4*gatP:3*gccR:1*ggaA*tacQ*agaA:1*ttgV:1*tggF:1*gatQ*ggcS:1+AR:1*agaN*tctN+Y:1*ttcG*caaP*ggtN*gatQ:1*ttcN*aagH:3*aggK:2*aggS:3*actR*tacF:1*attM*agaQ:2*ctgT:2*agaK*aacY:1*agaP*ggaA:3*acaM:4*gagD:1*gtcI:2*gtcR:2*caaN:2*gatN:6*ttgM*gaaN:2*tcaT:3*tctG*ttgV:2*ttaF:3*atcV:1*gccS*gcaS*attL*tctQ*catR*gacS*aagR*agaS*tctN:5*gcaS:3*ctcM*gttI:7*aagR:1*atcV*accI:5*gttM*tatH*cgtV:3*tccN:1*gatE:3*cctS:1*aagR:2*tatF*gatY:3*gctD:2*tttR*gagD:2*gctY:3*tcgT*attL*cgtK:2*accR:1*ttgI*atcV:3*agaQ:1*ccaG*caaK*tctN*gctP:4*atcE*aagE*gatE:1*gatG*aatP*aacG*tctL:1-ccacaaccagaggacgaggagatc:3*gaaK:1-cgtatgact*agtV*caaA*tcaE:1*aacR*cacS*attV*gagS:3*atcA*tcaP*gaaL*caaP*ctaV*accA*atgT*tctP*caaT*ttcP*caaS*cagI:1*attA*gctE*gttA:2*acaI-ggt*gttE*gatN:2*aacS+QDALLA*aatQ:2*accY:1*tacA:1*actQ:1*caaR*attL:1*ttgQ:2*gagA*cgtM:1*gctE*caaE*actR*ctaI*caaR:1*caaM:1*tatM*gctQ*gaaQ:2*agaQ-ctcttg:2*caaM:2*gtgR:2*caaM:1*caaA:1*cgtQ*ctcR:3*aatQ:1*attM*cgtA:2*tacM*caaA*tcgR*caaH:4*gtcM:6*ctaI:7*gaaD:4*ttgM:1*cagE:2*tacD*cagG:1*attV:4*actA:1*attL*gctN*accQ*actL*gctQ:1*tctT:1*actH:1*caaS*ttgA*ggaQ*tctA:2*gaaD:2*caaE*aatS:1*catQ*gaaQ:2*aatT*ttgM:1*aagR*aatQ:3*agtT:3*ttaR:2*caaS*ttaM:1*cagE:1*catY:1*aatA:2*aacK:1*tttL:1*aaaA*ttgT:7*caaK:3*gatE:1*cgtA:2*ttgM:1*aaaR:1*ctcM*aaaR*tcaH:2*gtcI:3*ggaD:2*cgtK:6*ttgY:2*gaaD*aagR*ttgA:3*aaaN*ggtK*gatE*gaaD*attV*gatE*atgR:2*ttaR*caaE*attL:1*gacM*ttgA*actQ*ttgD:2*gatA:1*tctK*gaaD:1*aacS*caaT*tcaG*tccA*aatD:2*actL*atcL*tttL*gctS*cagK:1*aagN*aagR:1*attL*actS*aatE:2*tcaN*caaA:1*agaK*caaM*caaK:1*tctR:1*tctL*tttD*aacE*ccaR*gacG*cttD*gaaD*caaS*ctaD:1*aagL*gatR*aagR*gaaL:2*ttgK:2*gttE*caaY*caaE*acaA*atgL*gatN*gatE:1*ctaF*gctN*gaaS:2+LH*aagQ*gctE:2*gagG:1*gatN:1*gccN*attG*gagG*gaaS-cagattgatcaagtcctagtt*gatT*catP:1*accA*aaaA:1*accH:1*ttgI*gttI:2*atcL:1*ctaE:2*cttS*aagQ:1*cttV:2*tccA*attL*tatF:2*gatE:10*tctT:2*ttcY:3*atcV:3*tcaA:1*acaE:2*actS*gacA:2*aacT:3*gatN:1*atcL:2*ggaE*ccaT*aatD:2*catY*tctA:3*cgtK*tcgT*ttgV:1*gatI*ttcY*actS*tctT*tctA*attV*tcaE*gatN:2*ttgS:4*attL*gttT:2*gttA:1*acaD:1*tccA*gagS:2*cagA*atcL*attV*tccN*tgtF:2*tccE*actS:2*gaaA*tctT:1*gagR*tttN:1*aaaI*tcaG:3*gatE*atgI*ttgI:1*ggtD+F*gatP*gaaL*gaaD*gcaD:3*gttR:2*gttT*ggtL:1*ttgM*agtN:2*gaaT*aaaA:2*gaaH:2*caaK:3*gatR*ttgM:3*tccT:1*cttI-ttgaacaataac*agaN*ggtM*gctS*gctG*aacD:1*agtG:1*attL:1*gatE*aatR:1*ttaM*caaA*aatK:2*caaD*tctA:1+A:1*acgA:2*ttgA*caaK:1*aatG*tctD:2*accK*aaaF*aagN:1+S:2*tcaL*atcK:1*caaT*attT:1*tttL*gaaQ*attL*aacH*aagE:1*atcA*ttaI*gctQ:2*attQ:2*acaI:1*tcaA:1*gagA*attA:2*aagR:2*attT*gctD*tcaA:1*gagN:4*aagQ*aacG*aagR:1*tctT*attS:1*aaaR*actA*gagQ*tatF:3*catN:1*cgtK:13*cttA:2*aaaN*gttI:3*actK:3*gttT:1*tcaR*ggtR:1*aaaS*acaG*catL:8*gaaN:5*gttA:12*ttaM:5*aacK:2*gctE*gcaC:2*agtV:5*aaaR*ggcN:2*aatK:3*gatE:2*actN*agaK*agaK*agtF-aat:1*ggtE*ggtL*gaaD*aatE*gatK*ttaV:2*tcaA*aagA:2*aatK:2*aatF:2*gttT:13*gcaD:1*attQ*gcaG:6*gagQ*atcM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gacE:1 +CM113344.1:1105885-1164023 miniprot mRNA 24983 28159 2582 + . ID=BC000025;Rank=1;Identity=0.5107;Positive=0.7036;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +CM113344.1:1105885-1164023 miniprot CDS 24983 28159 2582 + 0 Parent=BC000025;Rank=1;Identity=0.5107;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 diff --git a/results/2026-10-30_barnettozyma/data/GCA_049863505.1_orf_scan.tsv b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_orf_scan.tsv new file mode 100644 index 00000000..8b140319 --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/GCA_049863505.1_orf_scan.tsv @@ -0,0 +1,7 @@ +seg strand wstart wend aa pfam best_ref +s81 + 34006 35031 342 dic1:358 +s227 + 24962 28162 1067 ANTH:257:16-277,ENTH:19:15-129,I_LWEQ:196:909-1060 sla2:1191 +s406 + 33147 33284 46 Homeobox_KN:16:31-46,Homeodomain:13:21-46 460523_cbs-4732_MATog_MATa-MATalpha_MATalpha2:15 +s580 - 29079 29849 257 MATalpha_HMGbox:38:116-185 MTLalpha1:56 +s741 - 24287 24631 115 MH260578_SUI1:205 +s873 - 28243 28605 121 Homeobox_KN:30:84-113,Homeodomain:62:60-115 5476_sc5314_MTL_A_MTLA1:62 diff --git a/results/2026-10-30_barnettozyma/data/a2_benchmark.txt b/results/2026-10-30_barnettozyma/data/a2_benchmark.txt new file mode 100644 index 00000000..bead092d --- /dev/null +++ b/results/2026-10-30_barnettozyma/data/a2_benchmark.txt @@ -0,0 +1,3 @@ +curated MATa2 proteins (training + Pk): {'Deb_MTLA2': 256, 'Can_MTLA2': 234, 'Geo_MATA': 206, 'Yar_mata2': 194, 'Klu_MATA2': 185, 'Kom_MATa2': 181, 'Cla_MTLA2': 144, 'Pk_MATa2_202aa': 52} +124 segments >= 80 aa in the 66.6 kb window; best profile scores: [32, 32] median 32 +models: {'BC_MATa2': 82} diff --git a/results/2026-10-30_barnettozyma/fetch_windows.sh b/results/2026-10-30_barnettozyma/fetch_windows.sh new file mode 100644 index 00000000..7c990726 --- /dev/null +++ b/results/2026-10-30_barnettozyma/fetch_windows.sh @@ -0,0 +1,16 @@ +#!/bin/bash +# Fetch a window around each replay MTL/MATsc call (coordinates from results/2026-10-29_near_tie/flank_synteny.tsv) +# from NCBI nuccore. Window = call +/- 25 kb. Writes data/__-.fa +cd "$(dirname "$0")"; mkdir -p data +while IFS=$'\t' read -r asm contig start end; do + s=$((start-25000)); [ $s -lt 1 ] && s=1; e=$((end+25000)) + out="data/${asm}_${contig}_${s}-${e}.fa" + [ -s "$out" ] || curl -s "https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=nuccore&id=${contig}&rettype=fasta&seq_start=${s}&seq_stop=${e}" > "$out" + echo "$out $(grep -v '>' "$out" | tr -d '\n' | wc -c)"; sleep 0.4 +done <<'T' +GCA_023627795.1 JAMAIE010000005.1 614225 630853 +GCA_003706655.3 PPLX03000005.1 120524 137156 +GCA_003123585.1 QBLI01000035.1 9748 26373 +GCA_003123885.1 QBLJ01000026.1 125869 142498 +GCA_049863505.1 CM113344.1 1130885 1139023 +T diff --git a/results/2026-10-30_barnettozyma/gm.py b/results/2026-10-30_barnettozyma/gm.py new file mode 100644 index 00000000..9cb607b5 --- /dev/null +++ b/results/2026-10-30_barnettozyma/gm.py @@ -0,0 +1,25 @@ +"""Helpers for hand-curating gene models on a locus window: genome coordinates, splice-site and protein checks.""" +from Bio import SeqIO +from Bio.Seq import Seq +OFFSET = {} +def load(fa): + r = next(SeqIO.parse(fa, "fasta")); return str(r.seq).upper() +def sub(S, a, b, strand="+"): + """1-based inclusive window coordinates -> sequence on the given strand.""" + x = S[a - 1:b] + return x if strand == "+" else str(Seq(x).reverse_complement()) +def cds(S, exons, strand): + """exons: list of (a,b) window coords; returns the spliced CDS in transcript orientation.""" + ex = sorted(exons) if strand == "+" else sorted(exons, reverse=True) + return "".join(sub(S, a, b, strand) for a, b in ex) +def protein(S, exons, strand): + return str(Seq(cds(S, exons, strand)).translate()) +def introns(exons, strand): + ex = sorted(exons); return [(ex[i][1] + 1, ex[i + 1][0] - 1) for i in range(len(ex) - 1)] +def splice_sites(S, exons, strand): + out = [] + for a, b in introns(exons, strand): + t = sub(S, a, b, strand); out.append((a, b, b - a + 1, t[:2] + ".." + t[-2:], t[-45:-1] if False else "")) + return out +def find_orfs_around(S, a, b, strand): + return None diff --git a/results/2026-10-30_barnettozyma/make_records.py b/results/2026-10-30_barnettozyma/make_records.py new file mode 100644 index 00000000..9e6b5cdd --- /dev/null +++ b/results/2026-10-30_barnettozyma/make_records.py @@ -0,0 +1,70 @@ +"""Write the draft proposal for the B. californica PB4207 MAT locus (DRAFT, in this results directory only; nothing goes to db/). + +One combined record (both idiomorphs at one locus; Hession et al. 2025 describe MATa1+MATa2 on one side and MATalpha1+MATalpha2 on the other +of a 2.8 kb intergenic region, and consider the species homothallic). Gene models are OURS (models.gff3, tier 3). +Run from this directory: python3 make_records.py +""" +import re, yaml, collections +CONTIG, ACC, ASM = "JAMAIE010000005.1", "JAMAIE010000005.1", "GCA_023627795.1" +exons, meta = collections.defaultdict(list), {} +for l in open("models.gff3"): + if l.startswith("#"): continue + c = l.rstrip("\n").split("\t") + gid = re.search(r"(?:ID|Parent)=(BC_[^;.]+)", c[8]).group(1) + if c[2] == "gene": meta[gid] = (c[6], int(c[3]), int(c[4])) + if c[2] == "CDS": exons[gid].append((int(c[3]), int(c[4]))) +ROLE = {"dic1": "flanking_conserved", "sla2": "flanking_conserved"} +IDIO = {"MATa1": ["MATa"], "MATa2": ["MATa"], "MATalpha1": ["MATalpha"]} +GCLASS = {"MATalpha1": "alpha_box"} +order = sorted(meta, key=lambda g: meta[g][1]) +genes = [] +for i, gid in enumerate(order): + name = gid[3:]; strand, lo, hi = meta[gid] + ex = sorted(exons[gid]); ex = ex if strand == "+" else ex[::-1] + g = {"gene_index": i, "name": name, "role": ROLE.get(name, "core_MAT"), "present": True, "segment_index": 0, + "start": lo, "end": hi, "strand": strand, "order_in_locus": i, "exons": [{"start": a, "end": b} for a, b in ex]} + if name in GCLASS: g["gene_class"] = GCLASS[name] + genes.append(g) +lo = min(g["start"] for g in genes); hi = max(g["end"] for g in genes) + +NOTE = ("DRAFT, GENE MODELS ARE OURS (tier 3). B. californica strain PB4207 (Dalian Medical University, GCA_023627795.1, ctg_5 = JAMAIE010000005.1, " + "694,392 bp; the locus is 63 kb from one contig end). The one published description is Hession, Byrne, Wolfe & Butler, PLoS Genet 2025 " + "(PMID 41359681, doi 10.1371/journal.pgen.1011814): a centromere formed at the MAT locus of B. californica, whose 460 bp centromere sequence lies in a " + "2.8 kb intergenic region between MATa1 and MATa2 on one side and MATalpha1 and MATalpha2 on the other; all three Barnettozyma species appear homothallic. " + "The paper gives no gene models, coordinates or accessions for the locus (its UCD09 ONT assembly is not in the NCBI assembly database as of 2026-10-30; " + "the supplement S1 Table was not available). NO RNA-seq exists for the genus (ENA, 2026-10-30: only WGS and Hi-C), so every model is a prediction from " + "sequence: stop-to-stop six-frame ORFs, Pfam (Homeodomain, MATalpha_HMGbox, ANTH/I_LWEQ), phmmer against the curated Saccharomycotina MAT proteins, an " + "exhaustive one-intron GT..AG search scored with the curated Saccharomycotina MATa2 profile. WHAT AGREES WITH THE PAPER: MATa1 ends at 615,568 and MATalpha1 " + "starts at 618,402, a 2,833 bp intergenic region (paper: 2.8 kb); MATa2 and MATa1 are the divergent pair on one side. SLA2 and DIC1 flank the locus " + "(DIC1 7.4 kb from MATa2; SLA2 8.6 kb from MATalpha1). MODELS: SLA2 (1,046 aa, ANTH + I_LWEQ, single exon), DIC1 (316 aa, single exon), MATa1 (162 aa, " + "homeodomain, single exon), MATalpha1 (233 aa, alpha-box, zero-intron ORF whose C-terminus is uncertain: one-intron alternatives score the same), MATa2 (269 aa, " + "one 56 bp GTATGT..CTTAG intron at 614,112-614,167, 82 bits to the MATa2 profile against a background maximum of 32 over all 124 six-frame segments of the " + "window; no Pfam HMG_box hit, as for Komagataella). MATalpha2 IS NOT RECORDED: a homeodomain region (genome 620,370-620,700) follows MATalpha1 but no " + "complete gene model could be built; it is not recorded as present or absent. NAMING: Pfam cannot tell the MATa1 homeodomain from the MATalpha2 homeodomain; " + "the names follow position (the homeodomain gene next to the HMG-like gene on the MATa side is MATa1). The other three californica assemblies (UCD09 " + "GCA_003123585.1, UCD89 GCA_003123885.1, NRRL Y-17395 GCA_003706655.3) carry the same proteins at 99.6-100% identity, which is one strain family, not independent " + "evidence. B. discipulorum (GCA_049863505.1) has SLA2, MATa1, MATalpha1 and DIC1 at 45-86% identity but arranged differently and no MATa2 hit. " + "GENETIC CODE: standard (table 1), not tested. Details: results/2026-10-30_barnettozyma/README.md.") +tax = {"taxid": 36038, "lineage": "k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Phaffomycetales;f__Phaffomycetaceae;g__Barnettozyma;s__Barnettozyma_californica", + "lineage_resolved_date": "2026-10-30"} +CIT = [{"pmid": "41359681", "doi": "10.1371/journal.pgen.1011814"}] +rec = {"record_id": "36038_pb4207_MATbz_MATa-MATalpha", "record_version": 1, "taxonomy": tax, + "organism": {"species": "Barnettozyma californica", "strain": {"name": "PB4207", "known": True, "culture_collection_ids": ["PB4207"], "differs_from_sequenced": False}}, + "mating_type": {"locus_name": "MATbz", "idiomorphs": ["MATa", "MATalpha"], "system": "homothallic"}, + "locus": {"assembly_accession": ASM, "assembly_accession_basis": "strain_match_verified", "coordinate_provenance": "curator_derived", + "excluded_from_coordinate_benchmark": False, + "assembly_location": {"contig": CONTIG, "start": lo, "end": hi, "identity": 100.0}, + "core": {"completeness": "partial", "reference_orientation": "DIC1->MATa2->MATa1->MATalpha1->SLA2 (MATalpha2 not modelled)", "definition_note": NOTE, + "segments": [{"segment_index": 0, "sequence_source": {"type": "insdc_nucleotide", "accession": ACC, "seq_region": ACC}, + "start": lo, "end": hi, "contig_edge_distance": None, "sequence_checksum": None}]}, + "extended_flank": []}, + "genes": genes, + "evidence": {"locus_existence": {"tier": 3, "experimental_method": "published description of the locus (PMID 41359681) plus our own homology/ORF analysis of the PB4207 assembly; no RNA-seq", "citations": CIT}, + "boundaries": {"tier": 3, "experimental_method": "gene models predicted here from sequence (ORF, Pfam, phmmer, one-intron search); not an annotation of the deposit", "citations": []}, + "idiomorph_assignment": {"tier": 3, "experimental_method": "gene content and position: HMG-like MATa2 + homeodomain MATa1 on one side, alpha-box MATalpha1 on the other, separated by 2.8 kb (PMID 41359681); homeodomain names by position only", "citations": CIT}}, + "validation": {"status": "needs_review", "rejection_reason": None, "accession_resolved": False, "accession_resolved_date": None, + "accession_resolved_version": None, "sequence_match": {"status": "pass", "per_gene": [], "notes": "models derived from the assembly itself"}, "taxonomy_current": None}, + "curation": {"proposed_by": "claude-code-curation-agent", "proposal_dedupe_key": "41359681|36038|MATbz|MATa-MATalpha|PB4207", "reviewed_by": None, "reviewed_date": None}, + "model_provenance": None} +open(f"{rec['record_id']}.yaml", "w").write(yaml.safe_dump(rec, sort_keys=False, width=100)) +print(rec["record_id"], [(g["name"], g["start"], g["end"], g["strand"], len(g["exons"])) for g in genes]) diff --git a/results/2026-10-30_barnettozyma/models.gff3 b/results/2026-10-30_barnettozyma/models.gff3 new file mode 100644 index 00000000..dae3b764 --- /dev/null +++ b/results/2026-10-30_barnettozyma/models.gff3 @@ -0,0 +1,17 @@ +##gff-version 3 +JAMAIE010000005.1 curation gene 605238 606188 . - . ID=BC_dic1;Name=dic1 +JAMAIE010000005.1 curation mRNA 605238 606188 . - . ID=BC_dic1.t1;Parent=BC_dic1 +JAMAIE010000005.1 curation CDS 605238 606188 . - 0 ID=BC_dic1.cds;Parent=BC_dic1.t1 +JAMAIE010000005.1 curation gene 613590 614455 . - . ID=BC_MATa2;Name=MATa2 +JAMAIE010000005.1 curation mRNA 613590 614455 . - . ID=BC_MATa2.t1;Parent=BC_MATa2 +JAMAIE010000005.1 curation CDS 614168 614455 . - 0 ID=BC_MATa2.cds;Parent=BC_MATa2.t1 +JAMAIE010000005.1 curation CDS 613590 614111 . - 0 ID=BC_MATa2.cds;Parent=BC_MATa2.t1 +JAMAIE010000005.1 curation gene 615080 615568 . + . ID=BC_MATa1;Name=MATa1 +JAMAIE010000005.1 curation mRNA 615080 615568 . + . ID=BC_MATa1.t1;Parent=BC_MATa1 +JAMAIE010000005.1 curation CDS 615080 615568 . + 0 ID=BC_MATa1.cds;Parent=BC_MATa1.t1 +JAMAIE010000005.1 curation gene 618402 619103 . + . ID=BC_MATalpha1;Name=MATalpha1 +JAMAIE010000005.1 curation mRNA 618402 619103 . + . ID=BC_MATalpha1.t1;Parent=BC_MATalpha1 +JAMAIE010000005.1 curation CDS 618402 619103 . + 0 ID=BC_MATalpha1.cds;Parent=BC_MATalpha1.t1 +JAMAIE010000005.1 curation gene 627731 630871 . - . ID=BC_sla2;Name=sla2 +JAMAIE010000005.1 curation mRNA 627731 630871 . - . ID=BC_sla2.t1;Parent=BC_sla2 +JAMAIE010000005.1 curation CDS 627731 630871 . - 0 ID=BC_sla2.cds;Parent=BC_sla2.t1 diff --git a/results/2026-10-30_barnettozyma/models.py b/results/2026-10-30_barnettozyma/models.py new file mode 100644 index 00000000..a343931e --- /dev/null +++ b/results/2026-10-30_barnettozyma/models.py @@ -0,0 +1,54 @@ +"""Hand-curated gene models of the B. californica PB4207 MAT locus (JAMAIE010000005.1), from sequence alone (no RNA-seq exists for the genus). +Models are defined in window coordinates (data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.fa; genome = window + 589224) and checked here: +start codon, stop codon, canonical GT..AG introns, translation == the ORF used for scoring. Writes models.gff3, models_proteins.faa, models.tsv. +Evidence behind each model: README.md.""" +import sys +sys.path.insert(0, ".") +from gm import load, sub, protein, cds, introns +FA = "data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.fa" +CONTIG, OFF = "JAMAIE010000005.1", 589224 +S = load(FA) + +def first_M(seg_a, seg_b, strand): + """Single-exon model from a stop-to-stop segment: first ATG to the stop codon (stop included in the CDS).""" + p = protein(S, [(seg_a, seg_b)], strand); k = p.find("M") + if strand == "+": + a = seg_a + 3 * k; b = seg_b + 3 # stop codon follows the segment + else: + a = seg_a - 3; b = seg_b - 3 * k # stop codon precedes (lower coordinates) + return [(a, b)] + +MODELS = [ + # name, strand, exons (window coords), role, note + ("dic1", "-", first_M(16017, 17015, "-"), "flanking_conserved", "mitochondrial dicarboxylate carrier; single exon; phmmer 362 bits to DIC1 references"), + ("MATa2", "-", [(24944, 25231), (24366, 24887)], "core_MAT", + "HMG-box-like MATa2 (no Pfam HMG_box hit, as for Komagataella); ONE-INTRON MODEL chosen by exhaustive search (82 bits to the Saccharomycotina MATa2 profile vs 64 and 50 for the next donors); 56 bp intron GTATGT..CTTAG"), + ("MATa1", "+", first_M(25832, 26341, "+"), "core_MAT", "homeodomain (Pfam 53 bits; helix 3 ...WFTNKR...), single exon, divergent partner of MATa2"), + ("MATalpha1", "+", first_M(29172, 29876, "+"), "core_MAT", "alpha-box (MATalpha_HMGbox 33 bits, 62 bits to curated MATalpha1); zero-intron ORF; the C-terminus is uncertain (alternative one-intron models score the same)"), + ("sla2", "-", first_M(38510, 41662, "-"), "flanking_conserved", "single exon, ANTH + I_LWEQ, 1217 bits to curated SLA2"), +] +rows, gff, faa = [], ["##gff-version 3"], [] +for name, strand, exons, role, note in MODELS: + ex = sorted(exons) + p = protein(S, exons, strand) + ok_start = p.startswith("M"); ok_stop = p.endswith("*") and p.count("*") == 1 + spl = [] + for a, b in introns(exons, strand): + t = sub(S, a, b, strand); spl.append(f"{a + OFF}-{b + OFF} {len(t)}bp {t[:2]}..{t[-2:]}") + ga, gb = ex[0][0] + OFF, ex[-1][1] + OFF + rows.append((name, role, strand, ga, gb, len(exons), len(p) - 1, "yes" if ok_start else "NO", "yes" if ok_stop else "NO", "; ".join(spl) or "-", note)) + gid = f"BC_{name}" + gff.append(f"{CONTIG}\tcuration\tgene\t{ga}\t{gb}\t.\t{strand}\t.\tID={gid};Name={name}") + gff.append(f"{CONTIG}\tcuration\tmRNA\t{ga}\t{gb}\t.\t{strand}\t.\tID={gid}.t1;Parent={gid}") + order = ex if strand == "+" else ex[::-1] + phase = 0 + for a, b in order: + gff.append(f"{CONTIG}\tcuration\tCDS\t{a + OFF}\t{b + OFF}\t.\t{strand}\t{phase}\tID={gid}.cds;Parent={gid}.t1") + phase = (3 - ((b - a + 1) - phase) % 3) % 3 + faa.append(f">{gid} {CONTIG}:{ga}-{gb}({strand}) {len(p)-1} aa\n{p.rstrip('*')}\n") +open("models.gff3", "w").write("\n".join(gff) + "\n") +open("models_proteins.faa", "w").write("".join(faa)) +with open("models.tsv", "w") as f: + f.write("gene\trole\tstrand\tstart\tend\texons\taa\tM_start\tstop\tintrons\tnote\n") + for r in rows: f.write("\t".join(map(str, r)) + "\n") +for r in rows: print(*r[:10], sep="\t") diff --git a/results/2026-10-30_barnettozyma/models.tsv b/results/2026-10-30_barnettozyma/models.tsv new file mode 100644 index 00000000..eec1ca4e --- /dev/null +++ b/results/2026-10-30_barnettozyma/models.tsv @@ -0,0 +1,6 @@ +gene role strand start end exons aa M_start stop introns note +dic1 flanking_conserved - 605238 606188 1 316 yes yes - mitochondrial dicarboxylate carrier; single exon; phmmer 362 bits to DIC1 references +MATa2 core_MAT - 613590 614455 2 269 yes yes 614112-614167 56bp GT..AG HMG-box-like MATa2 (no Pfam HMG_box hit, as for Komagataella); ONE-INTRON MODEL chosen by exhaustive search (82 bits to the Saccharomycotina MATa2 profile vs 64 and 50 for the next donors); 56 bp intron GTATGT..CTTAG +MATa1 core_MAT + 615080 615568 1 162 yes yes - homeodomain (Pfam 53 bits; helix 3 ...WFTNKR...), single exon, divergent partner of MATa2 +MATalpha1 core_MAT + 618402 619103 1 233 yes yes - alpha-box (MATalpha_HMGbox 33 bits, 62 bits to curated MATalpha1); zero-intron ORF; the C-terminus is uncertain (alternative one-intron models score the same) +sla2 flanking_conserved - 627731 630871 1 1046 yes yes - single exon, ANTH + I_LWEQ, 1217 bits to curated SLA2 diff --git a/results/2026-10-30_barnettozyma/models_proteins.faa b/results/2026-10-30_barnettozyma/models_proteins.faa new file mode 100644 index 00000000..d9383df3 --- /dev/null +++ b/results/2026-10-30_barnettozyma/models_proteins.faa @@ -0,0 +1,10 @@ +>BC_dic1 JAMAIE010000005.1:605238-606188(-) 316 aa +MSSVTATTHSTATGAPIALQPAASANAKTITATKTPTTKWPFWYGGFGGAVACFCTHPLDLAKVRLQTAPLPKPSLFQMAFSILKADGITGIYSGLSAGLLRQATYSLTRFGAYEYLKESFVPADKMSSMAYLLPISMVSGAVGGIVGNPSDIVNIRMQNDTGLPIEQRRNYKNAFDGVARIVTEEGPSALFRGLSTNLVRGVLMTSSQVVTYDLAKEMLISFGMKKESFFTFFSSSLLAGLAATTICSPADVLKTRIMNASGTNHSVVHILTKALKDEGPSFMFRGWVPSFVRLGPNTIIIFLVVEELKRMRIGL +>BC_MATa2 JAMAIE010000005.1:613590-614455(-) 269 aa +MESLKIAHFKASAGNDSDLRIKKSILPFVSIPKGPSSGQKFTLTFEDSPSTSSKQYSNVARPRNQFIVARMILNKIVLANCNDTIANDDVSKIISIIWKTSSSPFQKYFKYLSLLEESWHEEHYPLYHYQPKVRSKTIKSLAPVLEQSRTMTGRISKCAPLSSLKKKSGFVNTSPHMMGTYVKLSTSSFRHSTVVSNVSINLVSKFRLTKGHTSGTTYKKRGPRSSKDDAYIKNNLWCLSGFDKTNYFEPLFYEDKIQNITLEDFKDYI +>BC_MATa1 JAMAIE010000005.1:615080-615568(+) 162 aa +MNQKLITGQYQRLLFNTLADKYGSEVLELIDYSVEKPYFLSKSYRVKSSTISVNERSATESNCSDISPSSHSPPEASSMNTSNVCSVSEAVMASPDSNNNIQQKKRRRHISKEARSLLEKAFETTKNPTRKERAIIAEKSGMTALQVRVWFTNKRMRTKTRI +>BC_MATalpha1 JAMAIE010000005.1:618402-619103(+) 233 aa +MPRKEVKAKWTSNPKKPYSAGFKLFSLPGLKHRNNALESKNPSKCTSPSNFQCLTFAATSLPKIPKLSADYERFIEGLKDHKAGQDSSTYIRQHLRRKRGPSRKRTKLNGFMAYRSFFSRNVATDQQGVLSSQLAKEWERNPEIQLVWKRYSTEYNLSVLSDRLPFFDWLLLVTSEPTCISENIYDNKDPGTFSRIQDVFNTNEADENHQFLKMSFDVSVIDPRIGQAFEHSI +>BC_sla2 JAMAIE010000005.1:627731-630871(-) 1046 aa +MSKTISESDLQTALRKATSADETAPKRKHVRACIVYTWDHKSSLSFWNALKILPIQNDEVQLFKALITIHKVLQEGHQSALAEGYRNLNWIDGLGRSIQGDGFKGYGRLIKEYVTYLIRKLQFHRNHRGFNGTFEYEEYISLVTVQNPDEGYEAIMDLMSLQDSLDDLQRLIFATISHDKRSECKISALVPLVTESYGIYKFITSMLRAIYRSTGSDDALEPLKQRYESQHARLFEFYADCSSIRYLTSLITIPRLPETAPNLMVKDESSVEEETPRPESRQTNLSEPISEQPTVSQFQQPIAAQPTGVDFWSNQQSSFEAEQIRLQQERDAQALQQQQYAEQQRILFEQQQVQQQEQQRIAQEQLLREQYQSQAQGRVAELERDLLALRGQYEQDQLLLQQYDQRVKALETEITATTQSATQQIGSKDELIQNLHEQITLWKNKYESLAKLYSQLRQEHLNLLNKFKKLQQKAASAQEAIDKREKLEKDLKSKNVELAGLIRERDRARLDLEKVKGGKGDEIDKLELQIRDLTLKLDDAERNQSSNLSTIFAQHKKEIEALENQLRQQSSAFNPELEQLLKDKDEELEILQQTMDDALVELAKAQKENETAIEEQIDQVLVDHLTKLTSLVDAILLSGLKRLQDALYELDSPMQAGNQNSSPEYLLSIIEKASSSAMDFGDSFNDFIIDGPNGDHAEIIRSLNDFTSSIADVLLNSKGIVRLVKSDAEADQFIATARATAEESEEFFKSLLSDSLEGEVEEKTDVVMAGNLAVQEKLQDLTQLTEDLAPRSNLLNSNVANLGDLVETELQNAAASISKASSQLTSLLSKDQDPSISQIDFEINKAIVSAAIAVTNSIELLIKAAIASQEEIVAKNKGSISKTEYYKKHNRWTEGLISAAKSVALSTKILIETADGVLSGNKSHEQLIVASHEVAASTVQLVAASRVKATFLSKTQDNLETASKNVTSACRSLVNQVQDILTQRSNGGSGENDVDYSKLTNHENKTVEMEQQVEILKLENALIAARKRLGEIRKYGYRDGDSSEEE diff --git a/results/2026-10-30_barnettozyma/orf_scan.py b/results/2026-10-30_barnettozyma/orf_scan.py new file mode 100644 index 00000000..d5489c46 --- /dev/null +++ b/results/2026-10-30_barnettozyma/orf_scan.py @@ -0,0 +1,54 @@ +"""Six-frame stop-to-stop segments of a locus window, searched with the Pfam MAT-relevant profiles +(HMG_box, HMG_box_2, Homeodomain, Homeobox_KN, MATalpha_HMGbox, ANTH, ENTH, I_LWEQ) and with phmmer against the curated MAT proteins. +Usage: orf_scan.py window.fa refs.faa Pfam-A.hmm lo hi (lo/hi: 1-based window coordinates to scan) +Prints a TSV: seg_id, strand, window_start, window_end, aa, pfam hits (name:bits:dom_start-dom_end in aa), best ref (name, bits).""" +import sys, pyhmmer +def nm(x): return x.decode() if isinstance(x, bytes) else x +from Bio import SeqIO +from Bio.Seq import Seq +from pyhmmer.easel import SequenceFile, Alphabet, TextSequence +fa, refs, pfam, lo, hi = sys.argv[1], sys.argv[2], sys.argv[3], int(sys.argv[4]), int(sys.argv[5]) +MINAA = int(sys.argv[6]) if len(sys.argv) > 6 else 40 +rec = next(SeqIO.parse(fa, "fasta")); S = str(rec.seq).upper() +alpha = Alphabet.amino() +segs = [] # (id, strand, wstart, wend, aa) +for strand in "+-": + s = S if strand == "+" else str(Seq(S).reverse_complement()) + L = len(S) + for fr in range(3): + aa = str(Seq(s[fr:len(s) - ((len(s) - fr) % 3)]).translate()) + pos = 0 + for part in aa.split("*"): + n = len(part) + if n >= MINAA: + a0 = fr + pos * 3; a1 = a0 + n * 3 # 0-based [a0, a1) on the oriented strand + ws, we = (a0 + 1, a1) if strand == "+" else (L - a1 + 1, L - a0) + if we >= lo and ws <= hi: + segs.append((f"s{len(segs)+1}", strand, ws, we, part)) + pos += n + 1 +seqs = [TextSequence(name=i.encode(), sequence=p).digitize(alpha) for i, st, ws, we, p in segs] +want = {"HMG_box", "HMG_box_2", "Homeodomain", "Homeobox_KN", "MATalpha_HMGbox", "ANTH", "ENTH", "I_LWEQ", "HMG_box_5", "HMG_box_4"} +profs = [] +with pyhmmer.plan7.HMMFile(pfam) as hf: + for h in hf: + if nm(h.name) in want: profs.append(h) + if len(profs) == len(want): break +dom = {} +for hits in pyhmmer.hmmsearch(profs, seqs, E=1e-2, bit_cutoffs=None): + pn = nm(hits.query.name) + for h in hits: + for d in h.domains.included if len(h.domains.included) else []: + dom.setdefault(nm(h.name), []).append(f"{pn}:{d.score:.0f}:{d.alignment.target_from}-{d.alignment.target_to}") + if not len(h.domains.included) and h.score > 12: + dom.setdefault(nm(h.name), []).append(f"{pn}~:{h.score:.0f}") +with SequenceFile(refs, digital=True, alphabet=alpha) as sf: db = sf.read_block() +best = {} +for hits in pyhmmer.phmmer(seqs, db, E=1e-2): + if len(hits): + h = hits[0]; best[nm(hits.query.name)] = (nm(h.name), h.score) +print("seg\tstrand\twstart\twend\taa\tpfam\tbest_ref") +for i, st, ws, we, p in segs: + br = best.get(i); d = dom.get(i, []) + if br or d: + nm = br[0].split("name=")[1].split("|")[0] if br and "name=" in br[0] else (br[0].split("|")[-1] if br else "") + print(i, st, ws, we, len(p), ",".join(d), f"{nm}:{br[1]:.0f}" if br else "", sep="\t") diff --git a/results/2026-10-30_barnettozyma/run_lift.sh b/results/2026-10-30_barnettozyma/run_lift.sh new file mode 100644 index 00000000..74724d5b --- /dev/null +++ b/results/2026-10-30_barnettozyma/run_lift.sh @@ -0,0 +1,5 @@ +#!/bin/bash +# Map the PB4207 models (models_proteins.faa) onto the other four windows with miniprot (splice-aware): identity and intron positions. HPCC. +export PATH=/bigdata/stajichlab/jstajich/projects/MATPredict/.pixi/envs/default/bin:$PATH +cd "$(dirname "$0")" +for f in data/*.fa; do b=${f%.fa}; miniprot -t4 --gff -P L --outs=0.5 "$f" models_proteins.faa > "$b.lift.gff" 2> "$b.lift.log"; done diff --git a/results/2026-10-30_barnettozyma/run_miniprot.sh b/results/2026-10-30_barnettozyma/run_miniprot.sh new file mode 100644 index 00000000..fe786e2d --- /dev/null +++ b/results/2026-10-30_barnettozyma/run_miniprot.sh @@ -0,0 +1,5 @@ +#!/bin/bash +# miniprot of the curated Saccharomycotina MAT/flank proteins (refs.faa) against each locus window. Run on the HPCC. +export PATH=/bigdata/stajichlab/jstajich/projects/MATPredict/.pixi/envs/default/bin:$PATH +cd "$(dirname "$0")" +for f in data/*.fa; do b=${f%.fa}; miniprot -t4 --gff -P BC "$f" refs.faa > "$b.miniprot.gff" 2> "$b.miniprot.log"; done diff --git a/results/2026-10-30_barnettozyma/run_miniprot_a2.sh b/results/2026-10-30_barnettozyma/run_miniprot_a2.sh new file mode 100644 index 00000000..80ae43fa --- /dev/null +++ b/results/2026-10-30_barnettozyma/run_miniprot_a2.sh @@ -0,0 +1,5 @@ +#!/bin/bash +# Sensitive miniprot of the seven curated Saccharomycotina MATa2 proteins plus P. kudriavzevii MATa2 against each window (HPCC) +export PATH=/bigdata/stajichlab/jstajich/projects/MATPredict/.pixi/envs/default/bin:$PATH +cd "$(dirname "$0")" +for f in data/*.fa; do b=${f%.fa}; miniprot -t4 --gff -P A2 --outs=0.3 --outc=0.2 -k5 -p 0.3 "$f" a2_refs.faa > "$b.a2.gff" 2> "$b.a2.log"; done diff --git a/results/2026-10-30_barnettozyma/run_orf_scans.sh b/results/2026-10-30_barnettozyma/run_orf_scans.sh new file mode 100644 index 00000000..cd916e2b --- /dev/null +++ b/results/2026-10-30_barnettozyma/run_orf_scans.sh @@ -0,0 +1,8 @@ +#!/bin/bash +# Domain/homology scan of the six-frame segments of each locus window (local; needs pyhmmer, Biopython, Pfam-A.hmm) +cd "$(dirname "$0")" +PY=${PY:-python3} +for f in data/*.fa; do + b=$(basename "$f" .fa); tag=$(echo "$b" | cut -d_ -f1,2) + PYTHONPATH=/Users/jstajich/projects/MATPredict/src $PY orf_scan.py "$f" ../2026-10-29_ss_records/refs.faa /Users/jstajich/db/funannotate/Pfam-A.hmm 5000 60000 30 > data/${tag}_orf_scan.tsv 2>&1 +done diff --git a/results/2026-10-30_barnettozyma/scan_one_intron.py b/results/2026-10-30_barnettozyma/scan_one_intron.py new file mode 100644 index 00000000..eb437689 --- /dev/null +++ b/results/2026-10-30_barnettozyma/scan_one_intron.py @@ -0,0 +1,75 @@ +"""Exhaustive one-intron (and zero-intron) gene-model search in a window region, scored with the curated Saccharomycotina +MATa2 profile and with phmmer against the curated MATa2 proteins. Usage: scan_one_intron.py window.fa strand lo hi [minaa] [model: HMM path or pfam:NAME] +Introns: GT..AG, 40-250 bp. Only ORFs that contain the splice junction and have >= minaa aa are scored. Prints the top 12.""" +import sys, pyhmmer +def nm(x): return x.decode() if isinstance(x, bytes) else x +from Bio import SeqIO +from Bio.Seq import Seq +from pyhmmer.easel import Alphabet, TextSequence +fa, strand, lo, hi = sys.argv[1], sys.argv[2], int(sys.argv[3]), int(sys.argv[4]) +minaa = int(sys.argv[5]) if len(sys.argv) > 5 else 70 +MODEL = sys.argv[6] if len(sys.argv) > 6 else "/Users/jstajich/projects/MATPredict/db/Ascomycota/classifiers/MATa2_saccharomycotina/MATa2_saccharomycotina.hmm" # path, or pfam:NAME +S = str(next(SeqIO.parse(fa, "fasta")).seq).upper() +R = S[lo - 1:hi] +T = R if strand == "+" else str(Seq(R).reverse_complement()) +def gpos(i): # index on T -> window coordinate + return lo + i if strand == "+" else hi - i +alpha = Alphabet.amino() +def load_model(): + if MODEL.startswith("pfam:"): + with pyhmmer.plan7.HMMFile("/Users/jstajich/db/funannotate/Pfam-A.hmm") as hf: + for h in hf: + if nm(h.name) == MODEL[5:]: return h + raise SystemExit("no such Pfam profile") + with pyhmmer.plan7.HMMFile(MODEL) as hf: return hf.read() +if MODEL.startswith("refs:"): + import re as _re + _, rfa, rx = MODEL.split(":", 2) + from pyhmmer.easel import SequenceFile + with SequenceFile(rfa, digital=True, alphabet=alpha) as sf: _db = [x for x in sf.read_block() if _re.search(rx, nm(x.name))] + from pyhmmer.easel import DigitalSequenceBlock + _db = DigitalSequenceBlock(alpha, _db) + hmm = None +else: + hmm = load_model() +cands = [] +CURJ = [None] +def orfs(spliced, junction): + for fr in range(3): + aa = str(Seq(spliced[fr:len(spliced) - ((len(spliced) - fr) % 3)]).translate()) + pos = 0 + for part in aa.split("*"): + a0 = fr + pos * 3; a1 = a0 + len(part) * 3 + if len(part) >= minaa and (junction is None or a0 < junction < a1): + cands.append((spliced, part, a0, junction, CURJ[0])) + pos += len(part) + 1 +orfs(T, None) +n = len(T) +gts = [i for i in range(n - 1) if T[i:i + 2] == "GT"] +ags = [j for j in range(1, n) if T[j - 1:j + 1] == "AG"] +import bisect +for i in gts: + for j in ags[bisect.bisect_left(ags, i + 40):bisect.bisect_right(ags, i + 250)]: + CURJ[0] = j + orfs(T[:i] + T[j + 1:], i) + # record intron with the candidate + cands[-1:] = cands[-1:] if cands and cands[-1][3] == i else cands[-1:] +# scoring: dedupe by protein +seen = {} +for sp, part, a0, junc, jend in cands: + seen.setdefault(part, (sp, a0, junc, jend)) +seqs = [TextSequence(name=str(k).encode(), sequence=p).digitize(alpha) for k, p in enumerate(seen)] +res = [] +keys = list(seen) +if hmm is None: + for hits in pyhmmer.phmmer(seqs, _db, E=1e-1): + if len(hits): res.append((hits[0].score, int(nm(hits.query.name)))) +else: + for hits in pyhmmer.hmmsearch(hmm, seqs, E=10): + for h in hits: + k = int(h.name if isinstance(h.name, str) else h.name.decode()); res.append((h.score, k)) +res.sort(reverse=True) +for sc, k in res[:12]: + p = keys[k]; sp, a0, junc, jend = seen[p] + print(f"{sc:.1f} bits len={len(p)} junction_idx_on_T={junc} cds_start_idx={a0}", ("intron window %d-%d len %d seq %s...%s" % (min(gpos(junc), gpos(jend)), max(gpos(junc), gpos(jend)), jend - junc + 1, T[junc:junc+8], T[jend-5:jend+1])) if junc is not None else "no intron") + print(" ", p) diff --git a/results/2026-10-30_barnettozyma/scan_two_introns.py b/results/2026-10-30_barnettozyma/scan_two_introns.py new file mode 100644 index 00000000..2e4ce11e --- /dev/null +++ b/results/2026-10-30_barnettozyma/scan_two_introns.py @@ -0,0 +1,58 @@ +"""Exhaustive search for a gene with up to two introns in a window region, for the divergent homeodomain MATalpha2 gene. +Donor GTA[ACT][GT] (yeast consensus GTATGT, relaxed), acceptor [CTA]AG, intron 35-200 bp. ORF must start with M, end at a stop, +be >= minaa, and contain every junction. Scored by a Pfam profile (default Homeodomain) and by phmmer against the curated alpha2 proteins. +Usage: scan_two_introns.py window.fa strand lo hi [minaa] [pfam name]""" +import sys, re, bisect, pyhmmer +from Bio import SeqIO +from Bio.Seq import Seq +from pyhmmer.easel import Alphabet, TextSequence, SequenceFile +def nm(x): return x.decode() if isinstance(x, bytes) else x +fa, strand, lo, hi = sys.argv[1], sys.argv[2], int(sys.argv[3]), int(sys.argv[4]) +minaa = int(sys.argv[5]) if len(sys.argv) > 5 else 120 +pf = sys.argv[6] if len(sys.argv) > 6 else "Homeodomain" +S = str(next(SeqIO.parse(fa, "fasta")).seq).upper() +R = S[lo - 1:hi]; T = R if strand == "+" else str(Seq(R).reverse_complement()) +gpos = (lambda i: lo + i) if strand == "+" else (lambda i: hi - i) +don = [m.start() for m in re.finditer(r"(?=GTA[ACT][GT])", T)] +acc = [m.end() for m in re.finditer(r"(?=[CTA]AG)", T)] # index just after AG = m.start()+3 ; use end of lookahead offset +acc = [m.start() + 3 for m in re.finditer(r"(?=[CTA]AG)", T)] # exclusive end of intron +introns = [(d, a) for d in don for a in acc[bisect.bisect_left(acc, d + 35):bisect.bisect_right(acc, d + 200)]] +alpha = Alphabet.amino() +def translate_orfs(chain, juncs): + out = [] + for fr in range(3): + L = len(chain) - ((len(chain) - fr) % 3) + aa = str(Seq(chain[fr:L]).translate()) + pos = 0 + for part in aa.split("*"): + k = part.find("M") + if k >= 0 and len(part) - k >= minaa: + a0 = fr + (pos + k) * 3; a1 = fr + (pos + len(part)) * 3 + if all(a0 < j < a1 for j in juncs): out.append(part[k:]) + pos += len(part) + 1 + return out +cands = {} +def add(prot, spec): cands.setdefault(prot, spec) +for (d, a) in introns: # one intron + ch = T[:d] + T[a:] + for p in translate_orfs(ch, [d]): add(p, ((d, a),)) +# two introns: restrict to pairs of introns that do not overlap, spacing >= 30 +import itertools +sel = introns +for i, (d1, a1) in enumerate(sel): + for (d2, a2) in sel: + if d2 >= a1 + 30 and d2 - a1 < 800: + ch = T[:d1] + T[a1:d2] + T[a2:] + for p in translate_orfs(ch, [d1, d1 + (d2 - a1)]): add(p, ((d1, a1), (d2, a2))) +keys = list(cands) +seqs = [TextSequence(name=str(k).encode(), sequence=p).digitize(alpha) for k, p in enumerate(keys)] +with pyhmmer.plan7.HMMFile("/Users/jstajich/db/funannotate/Pfam-A.hmm") as hf: + hmm = next(h for h in hf if nm(h.name) == pf) +res = [] +for hits in pyhmmer.hmmsearch(hmm, seqs, E=10): + for h in hits: res.append((h.score, int(nm(h.name)))) +res.sort(reverse=True) +for sc, k in res[:int(sys.argv[7]) if len(sys.argv) > 7 else 10]: + sp = cands[keys[k]] + print(f"{sc:.1f} {pf} bits, {len(keys[k])} aa, introns (window): " + "; ".join(f"{min(gpos(d),gpos(a-1))}-{max(gpos(d),gpos(a-1))} ({a-d} bp {T[d:d+6]}..{T[a-3:a]})" for d, a in sp)) + print(" ", keys[k]) diff --git a/results/2026-10-30_petasospora/.gitignore b/results/2026-10-30_petasospora/.gitignore new file mode 100644 index 00000000..b3e01da2 --- /dev/null +++ b/results/2026-10-30_petasospora/.gitignore @@ -0,0 +1,4 @@ +work/ +*.fna.gz +*.fa +*.fai diff --git a/results/2026-10-30_petasospora/36022_joy008_MATpf_MATa.yaml b/results/2026-10-30_petasospora/36022_joy008_MATpf_MATa.yaml new file mode 100644 index 00000000..4d2ff788 --- /dev/null +++ b/results/2026-10-30_petasospora/36022_joy008_MATpf_MATa.yaml @@ -0,0 +1,158 @@ +record_id: 36022_joy008_MATpf_MATa +record_version: 1 +taxonomy: + taxid: 36022 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Phaffomycetales;f__Wickerhamomycetaceae;g__Petasospora;s__Petasospora_fabianii + lineage_resolved_date: '2026-10-30' +organism: + species: Petasospora fabianii + strain: + name: JOY008 + known: true + culture_collection_ids: + - JOY008 + differs_from_sequenced: false +mating_type: + locus_name: MATpf + idiomorphs: + - MATa + system: heterothallic +locus: + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + assembly_accession: GCA_022641835.1 + assembly_accession_basis: sequence_link + assembly_location: + contig: CP048715.1 + start: 628449 + end: 644871 + identity: 100.0 + core: + completeness: complete + reference_orientation: DIC1->MATa2->MATa1->(six conserved genes)->SLA2 + definition_note: 'MATa idiomorph of P. fabianii isolate JOY008 (ecotype Missouri, St. Louis College of Pharmacy), complete + genome GCA_022641835.1, CP048715.1 (BioSample SAMN14051719). The type strain JCM 3601 (GCA_001599195.1, BCGI01000001.1:641,150-657,563) + carries the same MATa locus with 99-100% identical proteins (MATa1 and MATa2 one substitution each). The v0.7.0 MATsc + call (CP048715.1:630,345-685,843, 55 kb) is wider than the locus: the genes are DIC1 628,452-629,375 to SLA2 641,746-644,871. + GENE MODELS ARE OURS (tier 3, manual annotation; curator decision 2026-10-30). No RNA-seq exists for the species in + ENA/SRA (checked 2026-10-30), so the models are AUGUSTUS ab initio predictions (species models S. cerevisiae S288C, + C. guilliermondii, P. stipitis, K. lactis) chosen where several agree and where homology supports them; MATa2 is also + supported by the Saccharomycotina MATa2 profile (76.8 bits, above the 52.4 bits of P. kudriavzevii). Coordinates include + the stop codon. Intron signals: MATa2 72 bp GTAGGT..TACTAAC..TAG (strong); MATa1 97 bp GTACCA..AAAG (weak donor; an + upstream exon is required because the homeodomain open frame has no start codon, but the splice sites are unconfirmed). + The MATalpha1 model is a single exon of 305 aa; the depositor''s ONH67106 (254 aa, strain 65) is the same C-terminal + 185 aa plus a 69 aa exon joined across a 1,263 bp intron (GTCCTT..CCCCAG) that has no support here: the N-terminus is + unresolved. GENE NAMES by homology and position: MATa1 is the homeodomain protein (Pfam Homeodomain 64 bits, 66 bits + to Komagataella MATa1), MATa2 the HMG-box protein (divergent; no Pfam HMG_box hit above GA), MATalpha1 the alpha-box + protein (PF04769 36 bits, 407 bits to ONH67106). No MATalpha2 homeodomain gene was found in either alpha allele (only + a 57 aa Homeobox_KN fragment at 12-18 bits about 1.2 kb upstream of MATalpha1). LAYOUT: DIC1 (minus) - 0.5 kb (MATa) + or 5.0 kb (MATalpha) - MAT genes - six conserved non-MAT ORFs (409, 173, 589, 219, 465, 622 aa; identical in both alleles, + not recorded) - SLA2 (plus); SLA2 is therefore about 9.5 kb from the MAT genes. The alpha allele also carries an 800 + aa MutS_III-domain ORF between DIC1 and MATalpha1 that has no counterpart in the MATa allele (not recorded). No second + MAT cassette: miniprot of the MAT proteins finds no other MAT gene in any of the four assemblies, so the species looks + heterothallic with one idiomorph per genome. GENETIC CODE: standard (table 1). Details: results/2026-10-30_petasospora/README.md.' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: CP048715.1 + seq_region: CP048715.1 + start: 628449 + end: 644871 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] +genes: +- gene_index: 0 + name: dic1 + protein_accession: null + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 628449 + end: 629375 + strand: '-' + order_in_locus: 0 + exons: + - start: 628449 + end: 629375 +- gene_index: 1 + name: MATa2 + protein_accession: null + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 629850 + end: 630863 + strand: '-' + order_in_locus: 1 + exons: + - start: 630492 + end: 630863 + - start: 629850 + end: 630419 +- gene_index: 2 + name: MATa1 + protein_accession: null + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 631437 + end: 632136 + strand: + + order_in_locus: 2 + exons: + - start: 631437 + end: 631737 + - start: 631835 + end: 632136 +- gene_index: 3 + name: sla2 + protein_accession: null + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 641746 + end: 644871 + strand: + + order_in_locus: 3 + exons: + - start: 641746 + end: 644871 +evidence: + locus_existence: + tier: 3 + experimental_method: 'manual annotation: homology (curated Saccharomycotina MAT proteins, Pfam, MATa2 profile) and AUGUSTUS + ab initio models on the deposited assembly; no RNA-seq' + citations: &id001 [] + boundaries: + tier: 3 + experimental_method: AUGUSTUS ab initio gene models checked against homology; DIC1 and SLA2 are the flanking anchors, + MAT genes lie between them + citations: *id001 + idiomorph_assignment: + tier: 3 + experimental_method: 'gene content: MATa1 (homeodomain) with MATa2 (HMG-box) versus MATalpha1 (alpha box), at the same + DIC1-SLA2 position in different strains' + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: '' + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: manual|36022|MATpf|MATa + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/results/2026-10-30_petasospora/36022_tbrc-4498_MATpf_MATalpha.yaml b/results/2026-10-30_petasospora/36022_tbrc-4498_MATpf_MATalpha.yaml new file mode 100644 index 00000000..e4905bb2 --- /dev/null +++ b/results/2026-10-30_petasospora/36022_tbrc-4498_MATpf_MATalpha.yaml @@ -0,0 +1,144 @@ +record_id: 36022_tbrc-4498_MATpf_MATalpha +record_version: 1 +taxonomy: + taxid: 36022 + lineage: k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Phaffomycetales;f__Wickerhamomycetaceae;g__Petasospora;s__Petasospora_fabianii + lineage_resolved_date: '2026-10-30' +organism: + species: Petasospora fabianii + strain: + name: TBRC 4498 + known: true + culture_collection_ids: + - TBRC 4498 + differs_from_sequenced: false +mating_type: + locus_name: MATpf + idiomorphs: + - MATalpha + system: heterothallic +locus: + coordinate_provenance: curator_derived + excluded_from_coordinate_benchmark: false + assembly_accession: GCA_050575385.1 + assembly_accession_basis: sequence_link + assembly_location: + contig: CM115473.1 + start: 631646 + end: 651160 + identity: 100.0 + core: + completeness: complete + reference_orientation: DIC1->MATalpha1->(six conserved genes)->SLA2 + definition_note: 'MATalpha idiomorph of P. fabianii TBRC 4498 (xylitol-producing strain; PMID 40558965), chromosome-level + GCA_050575385.1, CM115473.1. The draft genome of strain 65 from fermented masau fruit (GCA_001983305.1, MPUK01000005.1:611,410-630,920; + PMID 28385833) carries the same locus with an identical MATalpha1 protein and holds the depositor''s ONH67106 (''Mating-type-like + protein ALPHA1'', 254 aa, MPUK01000005.1:616,190-616,396 + 617,660-618,217), which our model replaces. GENE MODELS ARE + OURS (tier 3, manual annotation; curator decision 2026-10-30). No RNA-seq exists for the species in ENA/SRA (checked + 2026-10-30), so the models are AUGUSTUS ab initio predictions (species models S. cerevisiae S288C, C. guilliermondii, + P. stipitis, K. lactis) chosen where several agree and where homology supports them; MATa2 is also supported by the + Saccharomycotina MATa2 profile (76.8 bits, above the 52.4 bits of P. kudriavzevii). Coordinates include the stop codon. + Intron signals: MATa2 72 bp GTAGGT..TACTAAC..TAG (strong); MATa1 97 bp GTACCA..AAAG (weak donor; an upstream exon is + required because the homeodomain open frame has no start codon, but the splice sites are unconfirmed). The MATalpha1 + model is a single exon of 305 aa; the depositor''s ONH67106 (254 aa, strain 65) is the same C-terminal 185 aa plus a + 69 aa exon joined across a 1,263 bp intron (GTCCTT..CCCCAG) that has no support here: the N-terminus is unresolved. + GENE NAMES by homology and position: MATa1 is the homeodomain protein (Pfam Homeodomain 64 bits, 66 bits to Komagataella + MATa1), MATa2 the HMG-box protein (divergent; no Pfam HMG_box hit above GA), MATalpha1 the alpha-box protein (PF04769 + 36 bits, 407 bits to ONH67106). No MATalpha2 homeodomain gene was found in either alpha allele (only a 57 aa Homeobox_KN + fragment at 12-18 bits about 1.2 kb upstream of MATalpha1). LAYOUT: DIC1 (minus) - 0.5 kb (MATa) or 5.0 kb (MATalpha) + - MAT genes - six conserved non-MAT ORFs (409, 173, 589, 219, 465, 622 aa; identical in both alleles, not recorded) + - SLA2 (plus); SLA2 is therefore about 9.5 kb from the MAT genes. The alpha allele also carries an 800 aa MutS_III-domain + ORF between DIC1 and MATalpha1 that has no counterpart in the MATa allele (not recorded). No second MAT cassette: miniprot + of the MAT proteins finds no other MAT gene in any of the four assemblies, so the species looks heterothallic with one + idiomorph per genome. GENETIC CODE: standard (table 1). Details: results/2026-10-30_petasospora/README.md.' + segments: + - segment_index: 0 + sequence_source: + type: insdc_nucleotide + accession: CM115473.1 + seq_region: CM115473.1 + start: 631646 + end: 651160 + contig_edge_distance: null + sequence_checksum: null + extended_flank: [] +genes: +- gene_index: 0 + name: dic1 + protein_accession: null + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 631646 + end: 632572 + strand: '-' + order_in_locus: 0 + exons: + - start: 631646 + end: 632572 +- gene_index: 1 + name: MATalpha1 + protein_accession: null + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 637540 + end: 638457 + strand: + + order_in_locus: 1 + exons: + - start: 637540 + end: 638457 +- gene_index: 2 + name: sla2 + protein_accession: null + role: flanking_conserved + present: true + locus_tag: null + segment_index: 0 + start: 648035 + end: 651160 + strand: + + order_in_locus: 2 + exons: + - start: 648035 + end: 651160 +evidence: + locus_existence: + tier: 3 + experimental_method: 'manual annotation: homology (curated Saccharomycotina MAT proteins, Pfam, MATa2 profile) and AUGUSTUS + ab initio models on the deposited assembly; no RNA-seq' + citations: &id001 + - pmid: '40558965' + doi: 10.3390/jof11060453 + - pmid: '28385833' + doi: 10.1128/genomeA.00064-17 + boundaries: + tier: 3 + experimental_method: AUGUSTUS ab initio gene models checked against homology; DIC1 and SLA2 are the flanking anchors, + MAT genes lie between them + citations: *id001 + idiomorph_assignment: + tier: 3 + experimental_method: 'gene content: MATa1 (homeodomain) with MATa2 (HMG-box) versus MATalpha1 (alpha box), at the same + DIC1-SLA2 position in different strains' + citations: *id001 +validation: + status: needs_review + rejection_reason: null + accession_resolved: false + accession_resolved_date: null + accession_resolved_version: null + sequence_match: + status: pass + per_gene: [] + notes: '' + taxonomy_current: null +curation: + proposed_by: claude-code-curation-agent + proposal_dedupe_key: manual|36022|MATpf|MATalpha + reviewed_by: null + reviewed_date: null +model_provenance: null diff --git a/results/2026-10-30_petasospora/README.md b/results/2026-10-30_petasospora/README.md new file mode 100644 index 00000000..056a1fa7 --- /dev/null +++ b/results/2026-10-30_petasospora/README.md @@ -0,0 +1,41 @@ +# Petasospora (Cyberlindnera) fabianii: our annotation of the MAT locus (2026-10-30) + +**Question.** The only public lead was ONH67106 "Mating-type-like protein ALPHA1" (strain 65, MPUK01000005.1, a depositor annotation with no paper on the locus), and the v0.7.0 replay called a 55 kb MATsc locus on GCA_022641835.1 (CP048715.1:630,345-685,843) with DIC1 beside it. The curator decided a manual-annotation record at **tier 3**. + +**Answer in one line.** The MAT locus is a small block between DIC1 and SLA2 at the same place in all four assemblies; JOY008 and JCM 3601 carry **MATa** (MATa1 homeodomain + MATa2 HMG-box), TBRC 4498 and strain 65 carry **MATalpha** (MATalpha1 alpha-box). It is not 55 kb: the genes span 16 kb (DIC1 to SLA2) and the MAT genes themselves 2.3 kb (MATa) or 0.9 kb (MATalpha). + +## Evidence available +- **No RNA-seq exists.** ENA has 24 runs for tax 36022 (checked 2026-10-30, `data/ena_runs.tsv`): all WGS or amplicon, none RNA-Seq. The models are therefore homology and ab initio gene prediction, not transcript-supported. +- Four assemblies, all aligned with miniprot (`pf_miniprot.slurm`; the 63 curated Saccharomycotina MAT proteins plus ONH67106 and the *Williopsis jadinii* HMG-box protein XP_020071302): JOY008 GCA_022641835.1 (complete genome, ecotype Missouri, SAMN14051719), TBRC 4498 GCA_050575385.1 (chromosome; xylitol strain, PubMed 40558965, [DOI](https://doi.org/10.3390/jof11060453)), strain 65 GCA_001983305.1 (draft from fermented masau fruit, PubMed 28385833, [DOI](https://doi.org/10.1128/genomeA.00064-17)), JCM 3601 GCA_001599195.1 (type strain, scaffold). According to PubMed, no paper describes the MAT locus of this species; the YJS4271 genome (PubMed 25103752, [DOI](https://doi.org/10.1128/genomeA.00638-14)) is not annotated for MAT. +- AUGUSTUS 3.5.0 (`pf_augustus.slurm`, `classify_aug.py`) with four yeast species models on the four 19-22 kb windows (`make_windows.py`), classified by phmmer against the references, Pfam-A and the Saccharomycotina MATa2 profile. `scan_window.py` independently scans all six frames. Models checked in `build_models.py` (start codon, stop, introns). + +## Layout and models (CDS coordinates include the stop codon; minus-strand exons listed in transcript order) +| allele (strain, contig) | gene | strand | CDS | aa | evidence | +|---|---|---|---|---|---| +| MATa (JOY008, CP048715.1) | dic1 | - | 628,449-629,375 | 308 | 374 bits to curated DIC1; 4 of 4 species models agree | +| | **MATa2** | - | 630,863-630,492 + 630,419-629,850 | 313 | HMG-box, Saccharomycotina MATa2 profile **76.8 bits** (P. kudriavzevii 52.4); intron 630,420-630,491 (72 bp) GTAGGT..TACTAAC..TAG; P. stipitis model | +| | **MATa1** | + | 631,437-631,737 + 631,835-632,136 | 200 | homeodomain: Pfam Homeodomain 64 bits, 66 bits to Komagataella MATa1; intron 631,738-631,834 (97 bp) GTACCA..AAAG (weak, see limits) | +| | sla2 | + | 641,746-644,871 | 1,041 | I_LWEQ + ANTH, 1,233 bits to curated SLA2; single exon, 4 of 4 models | +| MATalpha (TBRC 4498, CM115473.1) | dic1 | - | 631,646-632,572 | 308 | as above | +| | **MATalpha1** | + | 637,540-638,457 | 305 | alpha box PF04769 36 bits; 407 bits to ONH67106; single exon, 4 of 4 models, identical protein in strain 65 (MPUK01000005.1:617,300-618,217) | +| | sla2 | + | 648,035-651,160 | 1,041 | as above | + +Between the MAT genes and SLA2 lie six conserved non-MAT ORFs (409, 173, 589, 219, 465, 622 aa) in the same order in both alleles; SLA2 is therefore about 9.5 kb from the MAT genes. DIC1 is 0.5 kb from MATa2 in the MATa allele and 5.0 kb from MATalpha1 in the MATalpha allele (the 800 aa ORF of finding 2 lies between). `data/models.tsv`, `data/models_proteins.faa`, `data/aug_classified.tsv` hold everything; the JCM 3601 (MATa) and strain 65 (MATalpha) copies are in the same table. The two MATa copies differ by one amino acid each in MATa1 and MATa2. + +## Findings that matter for the database +1. **Heterothallic-looking, no second cassette.** No MAT gene other than the locus above is found by miniprot in any of the four assemblies (only unrelated TGL1 hits elsewhere), and each genome carries one idiomorph. +2. **The two idiomorphs are at one position** (DIC1 side) with different gene content: MATa = MATa2 + MATa1 (two genes, 2.3 kb); MATalpha = MATalpha1 (one gene). The alpha allele also has an 800 aa MutS_III-domain ORF (TBRC 4498 CM115473.1:633,233-635,626) between DIC1 and MATalpha1 that has no counterpart in the MATa allele; not recorded. +3. **The depositor's ONH67106 model is probably wrong at the N-terminus.** It joins a 69 aa exon (616,190-616,396) to the 185 aa alpha-box exon across a 1,263 bp intron with a poor donor (GTCCTT); the open frame upstream of the alpha box is continuous, giving a 305 aa single-exon protein that all four species models produce. The exon-1 sequence matches no reference. No RNA can settle it. +4. **MATalpha2 not found** in either alpha allele: only a 57 aa Homeobox_KN fragment at 12-18 bits about 1.2 kb upstream of MATalpha1 (TBRC 4498 636,200-636,370 minus strand). Treated as absent; the draft family makes MATalpha2 optional. +5. **MATsc vs MTL.** This locus has DIC1 0.5-5 kb away and SLA2 9.5 kb away, no PAP1/OBP1/PIK1 in the window: the SLA2-type layout, not MTL, and MATa1/MATa2 are not MATsc cassettes either. A scoped family is the natural home (`order_MATpf_proposal.yml`, scope species 36022, not applied). + +## Limits (tier 3 for these reasons) +- No transcript evidence: every intron, start and stop is prediction. MATa2's intron has the full yeast signal set and the profile is strong; **MATa1's intron has a weak donor (GTACCA)** and an upstream exon is only required because the homeodomain open frame (631,726-632,133) has no start codon; its N-terminal 100 aa match no reference. The K. lactis model puts the acceptor at 631,864 (127 bp), the S. cerevisiae model at 631,834 (97 bp, same donor); I used the latter. +- **Gene names are by homology and position**: homeodomain = MATa1 (it could in principle be an alpha2 in the other allele; none was found there), HMG-box = MATa2, alpha box = MATalpha1. +- MATa2 is a 313 aa protein with a divergent HMG box (no Pfam HMG_box hit above GA, as for Komagataella and Pichia). +- Taxon: NCBI Taxonomy lists the species as *Petasospora fabianii* (taxid 36022; synonyms *Cyberlindnera fabianii*, *Hansenula fabianii*), class Saccharomycetes, order Phaffomycetales, family Wickerhamomycetaceae. +- Strain provenance: JOY008 has no publication that I found; TBRC 4498 and strain 65 are cited; the type strain JCM 3601 is the independent MATa replicate. + +## Files +- Drafts (not in db/): `36022_joy008_MATpf_MATa.yaml`, `36022_tbrc-4498_MATpf_MATalpha.yaml`, written by `make_records.py` (metadata schema, gene-status and exon-bound checks pass; the gene vocabulary and idiomorph checks pass against `order_MATpf_proposal.yml` appended to `db/Ascomycota/order.yml`). `curate-db validate` (taxonkit, HPCC) has not been run. +- Scripts: `pf_miniprot.slurm`, `pf_augustus.slurm`, `summarize_miniprot.py`, `make_windows.py`, `scan_window.py`, `classify_aug.py`, `build_models.py`. Genome FASTAs and `work/` are not committed (`.gitignore`); `data/` is 780 KB. diff --git a/results/2026-10-30_petasospora/build_models.py b/results/2026-10-30_petasospora/build_models.py new file mode 100644 index 00000000..4868d79f --- /dev/null +++ b/results/2026-10-30_petasospora/build_models.py @@ -0,0 +1,52 @@ +"""Check and write the P. fabianii MAT-locus models: CDS from genome coordinates, translation, start/stop/splice-site checks. +Models are AUGUSTUS predictions (saccharomyces_cerevisiae_S288C for MATa1 and MATalpha1, pichia_stipitis for MATa2, any model for DIC1/SLA2) +selected by agreement across species models and homology; no RNA-seq exists for the species. Usage: build_models.py GENOME_DIR""" +import subprocess, sys, re +D = sys.argv[1] +G = {"CP048715.1": "GCA_022641835.1_ASM2264183v1", "BCGI01000001.1": "GCA_001599195.1_JCM_3601_assembly_v001", + "CM115473.1": "GCA_050575385.1_ASM5057538v1", "MPUK01000005.1": "GCA_001983305.1_ASM198330v1"} +# (record, contig, gene, strand, exons in genomic order, note) +M = [ + ("MATa:JOY008", "CP048715.1", "dic1", "-", [(628452, 629375)]), + ("MATa:JOY008", "CP048715.1", "MATa2", "-", [(629853, 630419), (630492, 630863)]), + ("MATa:JOY008", "CP048715.1", "MATa1", "+", [(631437, 631737), (631835, 632133)]), + ("MATa:JOY008", "CP048715.1", "sla2", "+", [(641746, 644868)]), + ("MATa:JCM3601", "BCGI01000001.1", "dic1", "-", [(641150, 642073)]), + ("MATa:JCM3601", "BCGI01000001.1", "MATa2", "-", [(642551, 643117), (643190, 643561)]), + ("MATa:JCM3601", "BCGI01000001.1", "MATa1", "+", [(644135, 644435), (644533, 644831)]), + ("MATa:JCM3601", "BCGI01000001.1", "sla2", "+", [(654441, 657563)]), + ("MATalpha:TBRC4498", "CM115473.1", "dic1", "-", [(631649, 632572)]), + ("MATalpha:TBRC4498", "CM115473.1", "MATalpha1", "+", [(637540, 638454)]), + ("MATalpha:TBRC4498", "CM115473.1", "sla2", "+", [(648035, 651157)]), + ("MATalpha:strain65", "MPUK01000005.1", "dic1", "-", [(611410, 612333)]), + ("MATalpha:strain65", "MPUK01000005.1", "MATalpha1", "+", [(617300, 618214)]), + ("MATalpha:strain65", "MPUK01000005.1", "sla2", "+", [(627795, 630917)]), +] +b = "TCAG"; aa = "FFLLSSSSYY**CC*WLLLLPPPPHHQQRRRRIIIMTTTTNNKKSSRRVVVVAAAADDEEGGGG" +C = {x + y + z: aa[16 * i + 4 * j + k] for i, x in enumerate(b) for j, y in enumerate(b) for k, z in enumerate(b)} +def rc(s): return s[::-1].translate(str.maketrans("ACGT", "TGCA")) +def tr(s): return "".join(C.get(s[i:i + 3], "X") for i in range(0, len(s) - 2, 3)) +def get(contig, a, b_): + return "".join(subprocess.check_output(["samtools", "faidx", f"{D}/{G[contig]}.fa", f"{contig}:{a}-{b_}"], text=True).split("\n")[1:]).upper() +out = open("data/models.tsv", "w"); fa = open("data/models_proteins.faa", "w") +out.write("record\tcontig\tgene\tstrand\texons\tcds_nt\taa\tstart\tstop_in_frame_end\tintron_signals\n") +for rec, c, g, st, ex in M: + seqs = [get(c, a, b_) for a, b_ in ex] + if st == "-": seqs = [rc(s) for s in seqs[::-1]]; ex_t = ex[::-1] + else: ex_t = ex + cds = "".join(seqs) + # next three bases after the last exon end (stop codon if not included) + last = ex[0][0] if st == "-" else ex[-1][1] + nxt = rc(get(c, last - 3, last - 1)) if st == "-" else get(c, last + 1, last + 3) + p = tr(cds) + has_stop = p.endswith("*") + stop_ext = (not has_stop) and tr(nxt) == "*" + sig = [] + for i in range(len(ex_t) - 1): # intron between consecutive exons in transcript order + if st == "+": a_, b_ = ex_t[i][1] + 1, ex_t[i + 1][0] - 1; intr = get(c, a_, b_) + else: a_, b_ = ex_t[i + 1][1] + 1, ex_t[i][0] - 1; intr = rc(get(c, a_, b_)) + sig.append(f"{a_}-{b_}({len(intr)}bp):{intr[:6]}..{intr[-6:]}") + internal = p.rstrip("*")[:-0 or None].count("*") + out.write("\t".join([rec, c, g, st, ";".join(f"{x}-{y}" for x, y in ex_t if True), str(len(cds)), str(len(p.rstrip('*'))), p[:1] + ("(ATG)" if cds[:3] == "ATG" else "(not ATG)"), + "stop_in_cds" if has_stop else ("stop_after" if stop_ext else f"NO_STOP(next={tr(nxt)})") + (f";internal_stops={internal}" if internal else ""), "|".join(sig)]) + "\n") + fa.write(f">{rec.replace(':', '_')}|{g}|{c}:{ex[0][0]}-{ex[-1][1]}({st})\n{p.rstrip('*')}\n") diff --git a/results/2026-10-30_petasospora/classify_aug.py b/results/2026-10-30_petasospora/classify_aug.py new file mode 100644 index 00000000..5ce17ae7 --- /dev/null +++ b/results/2026-10-30_petasospora/classify_aug.py @@ -0,0 +1,53 @@ +"""Parse AUGUSTUS GFF3 predictions on the MAT windows (windows.fa), convert to genome coordinates, and classify each predicted protein +(Pfam MAT domains, phmmer best reference, Saccharomycotina MATa2 profile). Usage: classify_aug.py data/aug_*.gff3 > table""" +import re, sys, pyhmmer +from pyhmmer.easel import SequenceFile, Alphabet, TextSequence +def _s(x): return x.decode() if isinstance(x, bytes) else x +win = {} +for l in open("data/windows.fa"): + if l.startswith(">"): + n, c, a, b = l[1:].split(); win[n] = (c, int(a), int(b)) +genes = [] # (species, window, id, strand, [cds], protein) +for fn in sys.argv[1:]: + sp = fn.split("aug_")[1].rsplit(".gff3", 1)[0] + cur = None; prot = None + for l in open(fn): + if l.startswith("#"): + if "protein sequence = [" in l: + prot = l.split("[", 1)[1].strip() + if prot.endswith("]"): prot = prot[:-1]; cur_p = prot; genes[-1][-1] = cur_p; prot = None + else: prot = prot + elif prot is not None: + t = l[1:].strip() + if t.endswith("]"): prot += t[:-1]; genes[-1][-1] = prot; prot = None + else: prot += t + continue + f = l.rstrip("\n").split("\t") + if len(f) < 9: continue + if f[2] == "transcript": + genes.append([sp, f[0], re.search(r"ID=([^;]+)", f[8]).group(1), f[6], [], ""]) + elif f[2] == "CDS": + genes[-1][4].append((int(f[3]), int(f[4]))) +alpha = Alphabet.amino() +ts = [TextSequence(name=f"{g[0]}|{g[1]}|{g[2]}".encode(), sequence=g[5]).digitize(alpha) for g in genes if g[5]] +with SequenceFile("data/all_refs.faa", digital=True, alphabet=alpha) as sf: db = sf.read_block() +best = {} +for hits in pyhmmer.phmmer(ts, db, E=1e-3): + if len(hits): best[_s(hits.query.name)] = (_s(hits[0].name), hits[0].score) +want = {"HMG_box", "HMG_box_2", "Homeodomain", "Homeobox_KN", "MATalpha_HMGbox", "I_LWEQ", "ANTH", "ENTH"} +dom = {} +with pyhmmer.plan7.HMMFile("/Users/jstajich/db/funannotate/Pfam-A.hmm") as hf: + for hits in pyhmmer.hmmscan(ts, hf, E=1e-1): + dom[_s(hits.query.name)] = [(_s(h.name), round(h.score, 1)) for h in hits if _s(h.name) in want] +with pyhmmer.plan7.HMMFile("/Users/jstajich/projects/MATPredict/db/Ascomycota/classifiers/MATa2_saccharomycotina/MATa2_saccharomycotina.hmm") as hf: + a2 = {} + for hits in pyhmmer.hmmsearch(list(hf), ts, E=1e-1): + for h in hits: a2[_s(h.name)] = round(h.score, 1) +print("species\twindow\tgene\tgstart\tgend\tstrand\tcds\taa\tbest_ref\tbits\tpfam\tMATa2_profile") +for g in genes: + k = f"{g[0]}|{g[1]}|{g[2]}" + c, a, b = win[g[1]] + cds = sorted((a + x - 1, a + y - 1) for x, y in g[4]) + br = best.get(k); nm = "" + if br: nm = (br[0].split("name=")[1].split("|")[0] if "name=" in br[0] else br[0])[-40:] + print("\t".join(map(str, [g[0], g[1], g[2], cds[0][0], cds[-1][1], g[3], ";".join(f"{x}-{y}" for x, y in cds), len(g[5]), nm, f"{br[1]:.0f}" if br else "", dom.get(k, ""), a2.get(k, "")]))) diff --git a/results/2026-10-30_petasospora/data/GCA_001599195.1_JCM_3601_assembly_v001.miniprot.gff b/results/2026-10-30_petasospora/data/GCA_001599195.1_JCM_3601_assembly_v001.miniprot.gff new file mode 100644 index 00000000..ab5328b5 --- /dev/null +++ b/results/2026-10-30_petasospora/data/GCA_001599195.1_JCM_3601_assembly_v001.miniprot.gff @@ -0,0 +1,86 @@ +##gff-version 3 +##PAF MH260578_SUI1 109 0 109 - BCGI01000001.1 3147266 623380 623707 267 327 0 AS:i:488 ms:i:488 np:i:103 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:109M cs:Z::2*tctN:1*cagE:2*aacK*actS:10*gatE:1*cagE*ccaV:30*agaK:2*cgtK:1*ttgI:2*gacE:24*gtcL:1*gttI:3*ttgM*accV*tccQ*cagT:1*gccG*ttgI*cagK:9 +BCGI01000001.1 miniprot mRNA 623378 623707 488 - . ID=PF000001;Rank=1;Identity=0.8165;Positive=0.9450;Target=MH260578_SUI1 1 109 +BCGI01000001.1 miniprot CDS 623378 623707 488 - 0 Parent=PF000001;Rank=1;Identity=0.8165;Target=MH260578_SUI1 1 109 +BCGI01000001.1 miniprot stop_codon 623378 623380 0 - 0 Parent=PF000001;Rank=1 +##PAF MH260578_SLA2 1072 29 1072 + BCGI01000001.1 3147266 654461 657563 1701 3156 0 AS:i:2753 ms:i:2753 np:i:774 fs:i:0 st:i:0 da:i:21 do:i:0 cg:Z:94M2D73M1D86M1I24M3I24M2I256M5I54M4D129M1I41M3I39M3I150M2D55M cs:Z:*tctT:1*cttI*attS*tcaT*tcgA*ttgI:3*actC*tctT*ctcP*gacE*gaaD:2*ccaS:18*agaK*cagD:1*ttcW*aacE*cagT*ctgM:1*ctgV*ctcV:1*gttL*cagK*ggtN*gatT*gaaD*gttT:4*acaA:2*accL:9*ggcP:3*aagV*gagG*gccG*atgF*cgtK:1*gctM*gatE:2*gagS*ggcN:1*ggaD*agaT*tcaL*gtcR*catT*ggtL:1-ggattc*aagR*ggcR*tacF*ggaQ*agaV:2*aagP:3*gtcN:1*ttgI:1*agaQ:2*aacR:2*aagH:17*gttR:2*caaS*aacD:1*gacN:3*gagD:5*atgT*actS:3*gctS:2*gatS*cttF*caaG:1*ctgD:3*agtT*atcV-tcc*catR*gacN*agaN*aagN:3*aagI:6*ttgI:10*attL*acgI:5*attM*cacY*cgcK*tccM*acgS*ggaD:1*gatA:1*gcaI:4*agaK*gaaS*cgcK*tatF:1*tctD:2*gccE:3*gagD:3*gacN:2*tcaN:5*tccT:5*agaK:2*gaaF*acaE:4+I:1*tccQ*aaaN:2*agtD*gacE*gtgN*gaaV*agtN*gaaL*agaP:1*gccL*tcaT*agaE:1*actK*tcgT*gtcA:2*ccaT*gttL+RHS:1*gaaT:1*atcF*tcgE*gaaQ*cagE*ccaN*actE*atgR*ggtQ*aacQ*ttcQ:1*cagA:3*tctT:4*gttM+VR*gatN*ttcA*tggF*aacV*accE:2*aacQ*gtgL*tttY:1*gccQ*gaaQ:2*agaQ:1*gcaE*agaQ*gaaQ*gccR:1*gaaA*aacE*atgL*cttQ:5*gctQ*gagL*caaA:1*cgtQ*attQ*tggY*tttW:4*gctR*gctQ:3*cagA:1*aggQ:3*gaaQ:3*cgcL:2*ttgA:1*aacR*cagH:1*gagQ:9*ttgI:1*gcaT*atgL*aggK*caaG:2*gagD:4*ttaM:2*cagS:3*agaK:1*aagQ*accA:2*cagN:1*attL:1*accS:5*actQ*cagE:2*ggtA:4*cttQ:2*agtL:4*ttgV*actE*ttaY:5*gatE:10*cagT:6*aacT:1*tttS:2*ttaI:12*cgtK:5*aagR:1*ttgM:1*tctA:2*gttI:3*ggtD:2*agaK:7*gacE:2*aaaR*attF:1*ggtN:1*aaaT*gacK*agcT*gagD*attL*gatQ*aagT:1*gagQ:1*cagE*atcK*aggD*gacE:1*acaE*ttgQ:2*gatA*gacT*tctL*gagD:1*agcA:2*tcgA:2*tcgT*tcgA*gtgI:1*gctN*tctQ:1*aagN*agaK:1*atgL*gaaQ*gacE:1*aagQ*gcaN:1+FKDVT:1*gagN*acaA*cagP:1*cgtD*gctE*ctgK*aacL*ccaK*gacQ:2*cagE*aagQ:1*aaaQ:2*gaaD*gagL:1*cttI:1*attM*gtgM:5*gatE*gccT*cttI*cagK*gagD:1*gccV*agaK*gtcT:1*ggaN*gagS*aacR*gatE:1*gctK*attS*aacE*gaaM:1*cttN*gatK*gcaL-gtcctgattgat*cacN*ctcS:2*ctcV*acaL*tcaG:1*gttI:4*gctK:3*aaaN:4*tccA*ttgV*tacF:10*caaL:6*ttgV:1*tccT*atcL*atcL:2*gccS*caaS*tcgG*tccL*gctV:4*aacS:3*gacN*ttcM*attL*gccV:2*ccaD:1*ggtA*gatN*cacE*tctA*gagL*atcV:1*agaD:2*aacT*gaaN:2*tctT:2*gccS:1*gttI:3*gccT:2*attL*gtgT:2*gtgT:1*actQ:1*agtD*gaaF*tctQ*aatD*cagD:2*gccD*gccT*tccA*agaK*tctD*accI:1*cagE*gaaI:2*gacV*ttcY:1*atgE:2*ctcY+H:1*gatN*atcL*cttV*gaaN*ggaK*actS*gagI*gaaD*gagD*aaaQ:1*gaaD*gttI:2*tccN:2*ttgI*aacD:3*aagI:2*gaaT:1*attL*gttQ*attL*gctV:1*aacS*cttM*gcaK*ccaT*agaP:1*gttS+KID:1*aagS*aacK*attV*aatD:1*gatE:1*ggtS:1*gctL:2*aacK:1*ttgM*aacE*tctH:1*gctS*tctR*gctV:1*acaE*tctE:1*gctS*gctV:2*aagS*gatS:3*tccQ*tctP:2+VSM:4*ggtI*gatE*atcV:2*gccS:2*tctA:2*attS*tccA*gttI*accI*aacT*tcaA:1*gctK*cttY:2*cagR:1*gccS:2*acaS:1*aatE:3*acgN*aaaN*aatG*cagR:2*ggtH*accS*cgtK*gccT*cagS:4*cacN:1*agaK:8*gctS:1*gctS*gttI:1*gccY:2*aagN*atcT:2*ggtR*actI:5*agtQ*tctN:1*aacH*accS*catN:3*attV:3*aatR:10*gctS:1*tcaA:3*gctS*acaQ:6*gatN*acgK:2*aatD:3*actK:1*actN*tctL:2*agaK*tctQ:2*aacD:2*cagN*gatK*attL*ctgI*aacI*cagG*agaK*acgH*aatE-ggtggt*gatV*cagE:1*gttI:1*ttcY:3*acgS*aacI:5*atgA:15*gctS:11*tatF*ggaS:1*agaK:1*ggtD:5 +BCGI01000001.1 miniprot mRNA 654462 657566 2753 + . ID=PF000002;Rank=1;Identity=0.5390;Positive=0.7357;Target=MH260578_SLA2 30 1072 +BCGI01000001.1 miniprot CDS 654462 657566 2753 + 0 Parent=PF000002;Rank=1;Identity=0.5390;Target=MH260578_SLA2 30 1072 +BCGI01000001.1 miniprot stop_codon 657564 657566 0 + 0 Parent=PF000002;Rank=1 +##PAF MH260578_TGL1 440 12 440 - BCGI01000003.1 1964508 1586344 1587619 585 1314 0 AS:i:911 ms:i:911 np:i:269 fs:i:0 st:i:0 da:i:27 do:i:51 cg:Z:15M2I11M2D4M5D23M1D51M4I229M7I20M1D29M1D33M cs:Z:*tcgA*ctcV:1*gagQ*tggL:2*gtcL*gtgI*gccF*tcgH*ttcK:1*gctG:1+EE:2*gagS*tttY*gtaF*atcQ*tccT*cttP*atcS*accN:1-gccatc:2*tcaN*gtgI-ctcgacctcgttaca:1*ttcA*atcV*caaS:1*gtgE*cacN*tctK:1*atcK*gttI*ccaS*gaaP*aagS*atcA:1*aacP*ggtL:1*ataE*ctcV*gagA:1-gct*gccT*aagH*aagC*atcL*acaH*aagQ*gatK:1*aggI:2*cctN*tcaD:1*caaV*cacD:1*ggaV:1*ctcA*tttH:2*caaK*attV*gatH*gatE:3*cctQ:1*gcaK:5*tgtS*cttI:3*aggL*ccaK*ctcS*aagS*gaaG*ggcD*gcaK:1+QNSR:9*atgT*tgcN:1*gatE*atcL*tggF*gttL*gtcL*aacG*atcE*aagN:2*agaK*aatC:3*attL:1*catV:1*atgQ:1*tttY:3*atgL:9*acaR:3*tccF:1*aaaS*ccaS*tcaT*catD*aagE*agaK:2*gacN*ttcY:1*atcL:5*tatF:3*gatN*tccT*atcV:4*tcaR*tacN:1*aagQ:1*caaD*gacS:1*acaS:10*gcaC*tttL:5*catN*gatH*aaaD:2*accK*cagK:1*aagN*ctcK:2*gctG:1*gctS:3*acaI:1*agtQ:2*cacN:1*caaP*ttcI*gtcA*gatS*acaF:1*atgV*aaaT:1*acaA:1*aacG*ttcL*atgL:1*ctgR*gccI:4*ctcA*ctcI:2*acaS*gcaV*tcaV:2*aaaQ*aatK*atcL*tgtM*tacG:1*ccaV:1*ttcY*tctE:1*atcV*atcV:1*cttK:1*gttL*cgcV*atgF:2*gacN:2*aatS*tacG:2*actK*catT*gatS:2*attK*gctV*gcaG*ttcY*tccP:1*ctcM*tacF:2*acaS:5*gttA:6*cgcD*aatS*ggtK*gcaR:2*ttgM:3*gacG+GASGSRL:1*tcaY:1*accS*aatG*gccT*ctcS*tctG*gctK*ccaT*ttcN:1*aacV:7-aat:1*aaaT*gtaT:1*atcC*aagL*ttgF*attV:2*tcaK*attS:1*tccM:1*gtgI:2*gatE*aagR*atgT:1*tcaD*ttaN:1*ccaS*gctC:2-aca*gatQ*gctT*gtaI*gcaG:1*ccaD*aatG*catY:2*ttgM:1*atcT*atcL:1*ggtA:1*cacQ*attV:2*ctcK:3*catV*ttgV*ttcL*aatQ*ttcY:1*ggtK*gtcQ +BCGI01000003.1 miniprot mRNA 1586345 1587619 911 - . ID=PF000003;Rank=1;Identity=0.4452;Positive=0.6142;Target=MH260578_TGL1 13 440 +BCGI01000003.1 miniprot CDS 1586345 1587619 911 - 0 Parent=PF000003;Rank=1;Identity=0.4452;Target=MH260578_TGL1 13 440 +##PAF MH260578_TGL1 440 79 440 - BCGI01000006.1 1133955 944577 945639 525 1101 0 AS:i:883 ms:i:883 np:i:237 fs:i:0 st:i:0 da:i:54 do:i:45 cg:Z:90M1I91M1I86M11I10M3D34M3D37M cs:Z::1*tttI*gcaV*tctD*cttI*gctV*gcaH:1*caaH:2*cagK:1*caaH:2*actI:3*tccK:5*actS*ctcI:2*gtcI*tttL*gaaK*aagS*gaaS*atcG*tccD*tgcK*cagP*aagQ*ggcN*aagS*cctR:1*gctV:1*ttcL:11*atcL:2*gacE*cgcN*cgtK*gatE:4*tatF:2*ttgV*gagD*aagQ:15+R*tccK:1*atcL*acaF:1*aatS*gagS*tctT:1*ccaE*cggK*tacF:1*gacN*ttcY:6*cacM*acaF:3*tctN*attT*atcV:4*gacR*ttcN:1*agcQ:1*ccaD:2*actS:7*acaS*acaA:1*atgC*tttL:2*atgL:1*attL:1*gagH:3*tctK:1*gtcI:1*ttcK:1*gttI*gctG:3*gtgA:4*ccaG:1*actN*cacN:1*ctaI*atcA*aagS*ccaF*ttcL:1+T*caaS*actA:1*tcaG*aatL*atcL*accY*aagR*gtgI:2*agcK*ggaR*cctA*ttaI:2*ttcS:1*aatV*cttF*gtcW*aatQ:1*gccL*atcM*ccgG*cttP*cctV:2*aagE*gccK*atcV*attV:1*gtcK:2*ggtV*atcF:3*accW*tcaK*ggaS*gcaG:2*agcK*tcgT*aagS:2*ctcK*gcaV:3*tacH*ttgM:2*gttP:4*tgtS:1*gtaA:6*agaD*cagS*caaK*tccR:1*cacQ*aagM*tacF+DEGGASGSRLV:1*aaaL*gctS*gctG*gttT:1*aaaG*atgK:1*gatN-gacctctgg*gagR:1*gagA*ctcS:1*gatP*atgT*aaaQ*aatT*ctgI*aaaT*gtgT:3*ttgF:3*gacK*gccS:1*atcM:1*gtcI:2*gatE*aagR:1*aggL*cagD*catN*ttcL-aagcagtca*aacS*ctgC:5*aagG*gtgI*agtD:11*gagQ:1*ttgE*tatK*acgK:2*gcaP*cctV:2*gagQ*aagY*attL*gaaK*gagQ +BCGI01000006.1 miniprot mRNA 944578 945639 883 - . ID=PF000004;Rank=2;Identity=0.4768;Positive=0.6458;Target=MH260578_TGL1 80 440 +BCGI01000006.1 miniprot CDS 944578 945639 883 - 0 Parent=PF000004;Rank=2;Identity=0.4768;Target=MH260578_TGL1 80 440 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATa1 181 123 178 + BCGI01000001.1 3147266 644660 644825 108 165 0 AS:i:187 ms:i:187 np:i:42 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:55M cs:Z::3*acaR*tcaH:1*accP*cctE*gaaS:3*tttL:2*agaK*gtgA:2*gctV:2*actF:3*acgK:2*aaaE*gttR:2*gaaR*aagE:2*ctcI*acgS:12*atgA:1*aacS:1 +BCGI01000001.1 miniprot mRNA 644661 644825 187 + . ID=PF000005;Rank=1;Identity=0.6545;Positive=0.7636;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 124 178 +BCGI01000001.1 miniprot CDS 644661 644825 187 + 0 Parent=PF000005;Rank=1;Identity=0.6545;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 124 178 +##PAF 5476_sc5314_MTL_A_MTLA1 210 142 198 + BCGI01000001.1 3147266 644660 644828 96 168 0 AS:i:167 ms:i:167 np:i:42 fs:i:0 st:i:0 da:i:-1 do:i:3 cg:Z:56M cs:Z::3*acaQ*tcaR*attL*accD*cctN*gaaS*tccT:5*agaK:2*aagE*gctK*aagN*aggK*actQ:2*agtR*acgR:2*aaaE*gttL:4*tgtH:1*ctcV*acgS*ccaL*ctgS:1*gttI:10*aacK:2 +BCGI01000001.1 miniprot mRNA 644661 644828 167 + . ID=PF000006;Rank=1;Identity=0.5714;Positive=0.7500;Target=5476_sc5314_MTL_A_MTLA1 143 198 +BCGI01000001.1 miniprot CDS 644661 644828 167 + 0 Parent=PF000006;Rank=1;Identity=0.5714;Target=5476_sc5314_MTL_A_MTLA1 143 198 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1019 7 1019 + BCGI01000001.1 3147266 654455 657554 1560 3129 0 AS:i:2422 ms:i:2422 np:i:724 fs:i:0 st:i:0 da:i:15 do:i:9 cg:Z:256M2D10M1D15M2D10M4D5M2I31M1I8M1D218M1I8M7D22M4D11M7D70M1I34M1D30M1I40M1I26M1I11M2I140M2D57M cs:Z::1*gacN*tctL:1*cttI*attQ*tcaA:5*actC*tctT*ctcA:1*gaaA*actA:21*cagE:1*ttcW*aacH*cagC:5*gttI:1*ggtS*gatN*gaaD*gttT:1*ctcI:5*accV:9*ggcP*tctT*gctC:1*aagI*gagG*gccG*atgY*cgtK:1*gctI*gatS:1*atcL:1*ggcS:1*ggaS:1*tcaF*gtcS*catN*ggtN*gatG*ggaT*ttcA*aagA:2*ggaT:7*gtcF:2*ttaE*agaQ:2*aacK:2*aagH*aacD:6*acaM:9*gttR:2*caaS*aacD:1*gacN:2*tacF:1*gccS:1*ctcM:2*atgL*actS:3*gctS:2*gatN:3*ctgV:2*gctS:2*tccR:1*gacT*agaS*aagE:3*aagV:2*gctS:3*ttgI*gttI:9*attL*acgI:3*aggK:1*attL*cacY*cgcK:1*acgS*ggaE:3*gcaV*ttgI*gaaA:2*agaK*gaaD:1*tatF*gagD*tctV:2*gccR:2*tacF:9*cgcK:3*tccT:1*attV:6*gaaS*acaS*gcaP:1-aacctc:1*tccL*aaaQ:2*agtE:1*gtgP*gaaQ*agtG-gaa*agaF:1*gccL*tcaV:1*caaE*actR:1*gtcS*gctV*acaS*ccaN*gttS*ccaE:1-ccaatc:1*gaaN*cagI:1*actR*atgS*ggtS:1*ttcI:1-cagccaattgca:5+AM:2*ttcS*tggF*aacV*accR:2*aacQ*gtgE*tttL:1*gccE*gaaQ:1*cagR*agaQ*cttA*gcaE*agaE*gaaR*gccR:1*gaaA*aacE*atgL:1*caaE:2*caaM+L:1*gctR*gagD:1*cagA:1*attF:1-ttt:1*cagE:2*gctR*gctQ:2*gaaV*cagS:1*aggE:3*gaaS:3*cgcA:2*ttgM:1*aacR:2*gagQ:1*agaK*gtcL:1*gagQ:2*cgtQ:3*gcaT*atgL*aggG*caaG:1*tacH:1*aacR:3*ttaM:1*cagE:7*accV:2*cagN:1*attL*gccR*accI*acaV:4*actE*cagE:2*ggtA*tctV:1*gatT*gagS*cttQ*ttgC*aacS*agtH:1*cagE:2*ttgV*actQ*ttaY:5*gatE:17*aacG:3*aagI*ttaV:9*gccS:1*gagD*cgtK:9*tctA:2*gttI:3*ggtD:2*agaK:9*gagA*aaaR:3*ggtN*aaaD*gacS*agcE*gagQ*attV*gatQ*aagM:1*gagR*ctcT:1*atcL:2*ttgM*acaE*ttgT:2*gatK*gacS*tctL:1*agaE*agcT*cagH:1*tcgQ:2*tcgA:1*gtgL:1*gctS*tctQ:1*aagS*agaK:1*atgI:1*gacE*ttgI:2*aaaS*cttA+S:1*acaL*cagK*tctE*cgtG*gctG:2-ccagaccttgaacagaagctc*aaaE:1*aagA*gaaQ*gagQ:1*cttV*gagQ*attM*gtgL:2*accA:3*gccT*cttM:4-agagtccaggga:1*aacQ*gatH*gagQ:2*aacE*gaaK*caaE*cttA*gatE-gcagtcctgattgatcacctc:1*aagR*ctcV*acaL*tcaR:1*gttI:1*gccT*atcL:1*gctK*tccV*ggtS*atcS*aaaD:1*attL*cagR*gacE*tccF*ttgV*tacF*gagN:1*gacE:2*atgV*cagY*gctS*ggtN*aatT*caaD*aacI*tcaP*tcaT*ccgA:3*cttG*tccT*atcM*atcL:1*aagR*gccV*caaS*tcgH*tccL*gctS:1*gacQ:1*gccS*aacT*tctT*ttcM*aatT:1*ttcY:3*ggtN*ccaM*aatK:1*gatE+S*cacE:1*gagG*atcL:1*agaK*tccA:1*aacI:1*ttcL:1*tctD:2*gccG*gatN*gttL*ctgF*ctcS:1*gccV:1*ggtA*attV*gtgL:1*cttD*gtgR:1*actD:1*agtV*gaaQ-tct*aatE*cagI:1*attV*gccE*gccG:1*agaK*tctD*accL:1*cagH*gaaM*tctV:1*gacL:1*cttF*atgE:1*ctcM:2*gatN*atcH:1*gaaL*ggaK*actL:1+I*gaaH:1*aaaR:1*gaaD*gttR:2*tccT:2*ttgI:4*aagV*cttI:1*gaaS:1*attM*gttQ*attV*gctV:1*aacV*cttF*gcaR*ccaD*agaP*tctG*gttR*ctcK*aagT*aacP*attV*aatS*ggtS:1+E:2*gatY*gctF*gttL:1*aacK:2*aacA*tctN*gccT*gctQ*tctA:1*atcV*acaE*tctK:2*gctG*catL:1*aagE*gatS*ctgM+S*cttT*aacR*tccH*tctK*ttcL*gatE*tcaV*tccQ*atcE:2+SL*gatE*atcV:1*aagE:2*ttaI:1*gctC:1*attS*tccA*gttI*accI:1*tcaA*atcV*gctK:2*attL*cagE:1*gccC:1*gagD*acaA:1*aatE:3*acgS*aaaR*aatG*cagR:2*ggtM*accS:2*cagL:4*cacN:10*gctS:1*gctQ:2*gccY*tccA:1*aagG:3*ggtR*actM:5*agtA*tctG*aagT:1*accS*catS:11*gctS*tccA:5*gctS*gccS:4*gctS*acaD*ttcL:2*aagQ*acgS*cagH*gatL*acgN:2*aatE:2*aagR*actK:3*gccS:1*agaK*tctM:2*aacT*caaK:1*cagR*gatS*attL:1-aaccag*agaL*acgS*aatD*ggtE*ggtS*gatN*cagK*gatT:2*ttcY:1*aagQ:2*aacA:3*aagR:1*atgA:1*atgL:10*aatS:2*gctS*tccH:10*tatF*ggaS:3*ggtE*gacE:1 +BCGI01000001.1 miniprot mRNA 654456 657554 2422 + . ID=PF000007;Rank=1;Identity=0.4986;Positive=0.6942;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +BCGI01000001.1 miniprot CDS 654456 657554 2422 + 0 Parent=PF000007;Rank=1;Identity=0.4986;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 187 129 184 + BCGI01000001.1 3147266 644660 644825 108 165 0 AS:i:187 ms:i:187 np:i:42 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:55M cs:Z::3*acaR*tcaH:1*accP*cctE*gaaS:3*tttL:2*agaK*gtgA:2*gctV:2*actF:3*acgK:2*aaaE*gttR:2*gaaR*aagE:2*ctcI*acgS:12*atgA:1*aacS:1 +BCGI01000001.1 miniprot mRNA 644661 644825 187 + . ID=PF000008;Rank=1;Identity=0.6545;Positive=0.7636;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 130 184 +BCGI01000001.1 miniprot CDS 644661 644825 187 + 0 Parent=PF000008;Rank=1;Identity=0.6545;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 130 184 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 294 0 294 - BCGI01000001.1 3147266 641149 642037 528 894 0 AS:i:888 ms:i:888 np:i:222 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:9M4D89M1I99M1I95M cs:Z:*agcM*tccN:1*ccaD*tcaK:2*acaK:1-cacaaagtcgac*gtgI:2*cgcS*cagA*aagN*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgK*atcV:1*aaaG*ccaD*actS:1*ttcV*caaS*atgL:1*aagF*tctK:1*ttgI*gttK*catT*gatE:1*ctcV:1*ggtA*gttA:1*tctA:2*tctT:1*ggtS:8*ctcT*acaA:6*tatK:3*aatI*gttM*gttT+D:1*gctT*gatR*aagG:1*tcaA:1*atgT*gcaF*tacQ:2*cctA*gttA:2*tttI*tctA:1*ggtA*attV:9*atcV:8*acaN*ggtS:1*gccP*ccaE*gatS:3*aacH:4*atcL:2*gtcL*atcL*agaK*gtgI*gctT:3*ggaN*actL*tctT:7*actP:2*gtgA:2*gtgI:3*tcgA:4*actS:5*gacK*tggL:1*ataV+E*tccN*ttcL*ggtS:1*aacD*aagP*gacK*tctT*attK*tccA:1*ttcH:5*cttI*tcaA:6*attV:18*gagQ:1*gtcS*gttF*cacG:2*accK*accD:1*ttcI*aaaS*aacR:2*cctL*tctG:6*gttT:1*agtA:1*gttI:4*aacH:2*atcL:2*ttgI*gttA*gttL:1*cagE:1*aagR*cacR:1*cgtK*gttI:1*atgL +BCGI01000001.1 miniprot mRNA 641147 642037 888 - . ID=PF000009;Rank=1;Identity=0.5906;Positive=0.7450;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +BCGI01000001.1 miniprot CDS 641147 642037 888 - 0 Parent=PF000009;Rank=1;Identity=0.5906;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +BCGI01000001.1 miniprot stop_codon 641147 641149 0 - 0 Parent=PF000009;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 305 0 305 - BCGI01000001.1 3147266 641149 642055 516 915 0 AS:i:860 ms:i:854 np:i:224 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ctcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*gcaP*ccaA*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*caaT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:2*tttL*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*actS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggtE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:4*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aacH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +BCGI01000001.1 miniprot mRNA 641147 642055 854 - . ID=PF000010;Rank=1;Identity=0.5639;Positive=0.7344;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 1 305 +BCGI01000001.1 miniprot CDS 641147 642055 854 - 0 Parent=PF000010;Rank=1;Identity=0.5639;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 1 305 +BCGI01000001.1 miniprot stop_codon 641147 641149 0 - 0 Parent=PF000010;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1045 0 1045 + BCGI01000001.1 3147266 654446 657563 1818 3156 0 AS:i:2981 ms:i:2981 np:i:800 fs:i:0 st:i:0 da:i:6 do:i:0 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D51M3D4M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tctA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tccN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcgE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*aacR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aaaR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ttgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*attV:1*ggtD*gatN*cttV:1*gatE*gctL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:6*tccA:2*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*acgN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gccY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE*gttI:1*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH-gatggtgac*agtA:3 +BCGI01000001.1 miniprot mRNA 654447 657566 2981 + . ID=PF000011;Rank=1;Identity=0.5760;Positive=0.7605;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +BCGI01000001.1 miniprot CDS 654447 657566 2981 + 0 Parent=PF000011;Rank=1;Identity=0.5760;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +BCGI01000001.1 miniprot stop_codon 657564 657566 0 + 0 Parent=PF000011;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1044 0 1044 + BCGI01000001.1 3147266 654446 657551 1809 3144 0 AS:i:2977 ms:i:2977 np:i:797 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D54M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tctA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tccN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcgE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*aacR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aaaR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ttgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*attV:1*ggtD*gatN*cttV:1*gatE*gctL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:6*tccA:2*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*acgN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gccY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE*gttI:1*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +BCGI01000001.1 miniprot mRNA 654447 657551 2977 + . ID=PF000012;Rank=1;Identity=0.5754;Positive=0.7605;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +BCGI01000001.1 miniprot CDS 654447 657551 2977 + 0 Parent=PF000012;Rank=1;Identity=0.5754;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 305 0 305 - BCGI01000001.1 3147266 641149 642055 516 915 0 AS:i:860 ms:i:854 np:i:224 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ctcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*gcaP*ccaA*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*caaT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:2*tttL*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*actS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggtE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:4*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aacH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +BCGI01000001.1 miniprot mRNA 641147 642055 854 - . ID=PF000013;Rank=1;Identity=0.5639;Positive=0.7344;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 1 305 +BCGI01000001.1 miniprot CDS 641147 642055 854 - 0 Parent=PF000013;Rank=1;Identity=0.5639;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 1 305 +BCGI01000001.1 miniprot stop_codon 641147 641149 0 - 0 Parent=PF000013;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 305 0 305 - BCGI01000001.1 3147266 641149 642055 516 915 0 AS:i:860 ms:i:854 np:i:224 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ctcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*gcaP*ccaA*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*caaT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:2*tttL*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*actS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggtE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:4*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aacH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +BCGI01000001.1 miniprot mRNA 641147 642055 854 - . ID=PF000014;Rank=1;Identity=0.5639;Positive=0.7344;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 1 305 +BCGI01000001.1 miniprot CDS 641147 642055 854 - 0 Parent=PF000014;Rank=1;Identity=0.5639;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 1 305 +BCGI01000001.1 miniprot stop_codon 641147 641149 0 - 0 Parent=PF000014;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1044 0 1044 + BCGI01000001.1 3147266 654446 657551 1809 3144 0 AS:i:2977 ms:i:2977 np:i:797 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D54M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tctA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tccN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcgE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*aacR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aaaR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ttgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*attV:1*ggtD*gatN*cttV:1*gatE*gctL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:6*tccA:2*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*acgN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gccY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE*gttI:1*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +BCGI01000001.1 miniprot mRNA 654447 657551 2977 + . ID=PF000015;Rank=1;Identity=0.5754;Positive=0.7605;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +BCGI01000001.1 miniprot CDS 654447 657551 2977 + 0 Parent=PF000015;Rank=1;Identity=0.5754;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 287 20 287 - BCGI01000001.1 3147266 641149 641944 492 801 0 AS:i:835 ms:i:835 np:i:206 fs:i:0 st:i:0 da:i:129 do:i:0 cg:Z:71M1I99M1I95M cs:Z::18*ccgK*atcV:1*aaaG*ccaD*actS:1*ttcV*caaS*atgL:1*aagY*tctK:1*ttgV*gttK*catT*gatE:1*ctcV:1*ggtA*gttA:1*tctA:4*ggtS:8*ctcT*acaA:6*tatK:2*gagG*aatI*gttM*gttT+D:1*gctT*gatK*aagG:1*tcaA:1*atgT*gcaF*tacQ:2*cctA*gttA:2*tttI*tctA:1*ggtA*attV:9*atcV:8*acaN*ggtS:1*gccP*ccaE*gatS:3*aacH:4*atcL:2*gtcL*atcL*agaK*gtgI*gctT:3*ggaN*actI*tctT:7*actP:2*gtgA:2*gtgI:3*tcgA:4*actS:5*gacK*tggL:1*ataV+E*tccN*ttcL*ggtS:1*aacD*aagP*gacK*tctT*attK*tccA:1*ttcH:5*cttI*tcaA:6*attV:18*gagQ:1*gtcS*gttF*cacG:2*accK*accD:1*ttcI*aaaS*aacR:2*cctL*tctG:6*gttT:1*agtA:1*gttI:4*aacH:2*atcL:2*ttgI*gttA*gttL:1*cagE:1*aagR*cacR:1*cgtK*gttI:1*atgL +BCGI01000001.1 miniprot mRNA 641147 641944 835 - . ID=PF000016;Rank=1;Identity=0.6142;Positive=0.7715;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 21 287 +BCGI01000001.1 miniprot CDS 641147 641944 835 - 0 Parent=PF000016;Rank=1;Identity=0.6142;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 21 287 +BCGI01000001.1 miniprot stop_codon 641147 641149 0 - 0 Parent=PF000016;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 181 123 178 + BCGI01000001.1 3147266 644660 644825 108 165 0 AS:i:187 ms:i:187 np:i:42 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:55M cs:Z::3*acaR*tcaH:1*accP*cctE*gaaS:3*tttL:2*agaK*gtgA:2*gctV:2*actF:3*acgK:2*aaaE*gttR:2*gaaR*aagE:2*ctcI*acgS:12*atgA:1*aacS:1 +BCGI01000001.1 miniprot mRNA 644661 644825 187 + . ID=PF000017;Rank=1;Identity=0.6545;Positive=0.7636;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 124 178 +BCGI01000001.1 miniprot CDS 644661 644825 187 + 0 Parent=PF000017;Rank=1;Identity=0.6545;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 124 178 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 440 0 440 + BCGI01000001.1 3147266 656210 657554 591 1362 0 AS:i:847 ms:i:847 np:i:296 fs:i:0 st:i:0 da:i:90 do:i:9 cg:Z:15M4D11M7D70M1I34M1D30M1I40M1I37M3I140M2D57M cs:Z:*gagQ*attM*gtgL:2*accA:3*gccT*cttM:4-agagtccaggga:1*aacQ*gatH*gagQ:2*aacE*gaaK*caaE*cttA*gatE-gcagtcctgattgatcacctc:1*aagR*ctcV*acaL*tcaR:1*gttI:1*gccT*atcL:1*gctK*tccV*ggtS*atcT*aaaD:1*attL*cagR*gacE*tccF*ttgV*tacF*gagN:1*gacE:2*atgV*cagY*gctS*ggtN*aatT*caaD*aacI*tcaP*tcaT*ccgA:3*cttG*tccT*atcM*atcL:2*gccV*caaS*tcgH*tccL*gctS:1*gacQ:1*gccS*aacT*tctT*ttcM*aatT:1*ttcY:3*ggtN*ccaM*aatR:2+S*cacE:1*gagG*atcL:1*agaK*tccA:1*aacT:3*tctD:2*gccG*gatN*gttL*ctgF*ctcS:1*gccV:1*ggtA*attV*gtgL:1*cttD*gtgR:1*actD:1*agtV*gaaQ-tct*aatE*cagI:1*attV*gccE*gccG*tccA*agaK*tctD*accL:1*cagH*gaaM*tctV:1*gacL:1*cttF*atgE:1*ctcM:3*atcH:1*gaaL*ggaK*actL:1+I*gaaH:1*aaaR:1*gaaD*gttR:2*tccT:2*ttgI:4*aagV*cttI:1*gaaS:1*attM*gttQ*attV*gctV:1*aacV*cttF*gcaR*ccaD*agaP*tctG*gttR*ctcK*aagS*aacP*attV*aatS*ggtS:1+E:2*gatY*gctF*gttL:1*aacK:2*aacA*tctN*gccT*gctQ*tctA:1*atcV*acaE*tctK:2*gctD*catL:1*aagE*gatS*ctgM*cttS*aacT*tccR*tctH*ttcK*gatL*tcaE*tccV*atcQ*gacE:1+GSL*gatE*atcV:1*aagE:2*ttaI:1*gctC:1*attS*tccA*gttI*accI:1*tcaA*atcV*gctK:2*attL*cagE:1*gccC:2*acaA:1*aatE:3*acgS*aaaR*aatG*cagK:2*ggtL*accS:2*cagL:4*cacN:10*gctS:1*gctQ:2*gccY*tccA:1*aagG:3*ggtR*actM:5*agtA*tctG*aagT:1*accS*catS:11*gctS*tccA:5*gctS*gccS:4*gctS*acaD*ttcL:2*aagQ*acgS*cagH*gatL*acgN:2*aatE:2*aagR*actK:3*gccS:1*agaK*tctM:2*aacT*caaK:1*cagR*gatS*attL:1-aaccag*agaL:1*aatD*ggtE*ggtS*gatN*cagK*gatT:2*ttcY:1*aagQ:2*aacA:3*aagR:1*atgA:1*atgL:10*aatS:2*gctS*tccQ:10*tatF*ggaS:3*ggtE*gacE:1 +BCGI01000001.1 miniprot mRNA 656211 657554 847 + . ID=PF000018;Rank=1;Identity=0.4339;Positive=0.6520;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +BCGI01000001.1 miniprot CDS 656211 657554 847 + 0 Parent=PF000018;Rank=1;Identity=0.4339;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 671 0 671 + BCGI01000001.1 3147266 654446 657563 840 2229 0 AS:i:1045 ms:i:1076 np:i:392 fs:i:0 st:i:0 da:i:6 do:i:0 cg:Z:94M2I11M12I73M6I2M2I90M6I11M18I10M4I2M11I2M4I18M6I30M2D30M2I39M22D45M7D12M1D15M312U6D25M21D14M7D27M798N9M6D38M cs:Z:*aagM*acaS*atcR*tccE*gacE*tctQ*gacT:1*attE*tcaK:1*ttgI:3*actL:1*ctcG*gacG*gaaD*actV:20*agaK*cagT:3*cagL:3*ctcQ:1*gttY*cagA*ggtT:2*gttL:4*acaA:1*atcV*accL*attL:8*ggcP:3*aagV:1*gccG*atgI:2*gctI:4*ggcS:1*ggaS:1+IF:2*catD*ggtS:1*ggaD*ttcG*aagS*ggcK:2+SSRMRYIGDEYA*agaK*ctcA:1*aagG:3*gtcF*tatF:1*ttaV*agaE:2*aacQ:3*aacS:1*cgtK:4*acaI:10*accS:1*caaS*aacD:1*gacN:4*gccT*attV:4*actN:1*caaE:1*gctV*ctgA:1*gatN:2*agaK:4*agtG:2*catN*gacR*agaK+SGFGSF*aagS:1+GL*gagD:1*aagR*atcG*tctA*gctV:1*gtgI:3*gctS:1*tcaT:3*tacF:2*attV:3*ctcM*aggK:1*attL*cacY*cgcK*tccQ*acgM:1*tctD*gatY*gagD:2*gaaS*cctG:1*agaF*gaaD:2*gagT*tctE:1*catC:3*tacN:3*gctF:2*tccN*tcaT*atcV*cgcQ*tacP:1*actK*tccN:5*agaT:2*gaaS*acaD*gcaP*ccaM*aacS*ctcR:1*tccI*aaaD:2*agtD:1*gtgA:1+VNDVVF*agtE*gaaK:1*ccgS*gccK:1*agaS*caaV*actS:1*gtcL+PSRTPTRENLRDPSPVHE:1*acaP:2*ccaA*gaaR:1*atcA*tcgE*gaaV+RSLS:2+AAPVVPIATGV*actV:1+PVVT:1*aacY*ttcS:1*cagP:1*attL*gcaQ*tctV*cagV:3*gttA*gatE:2*aacS+IPSTSA*accP*caaT*caaR*aacS:1*tttV*gaaA*gccS*gaaH*cagD*cagD*agaL:1*gcaI*agaE*gaaQ*gccQ*caaK*gaaQ*aacQ*atgQ*cttI:4*cagY:1*gagL:1-cagcgt*attL*tggE*tttH*gaaQ*cagK:1*cagL*gctQ*gctL:1*gctQ:3*aggA:1*gccL:1*gaaQ*cagR*cttI*ttgQ*cgcQ*gatQ*cagE*ttgM*cagE*aacK*cagE:1+LI*gagQ*ggaQ*agaK*gtcT:1*gagS:1*gaaQ*cgtN:1*ttgI*ctcM*gcaS*atgF*aggK*caaN:3*aacR:2*ttgV*ttaM:1*cagE:3*caaK:2*aagQ*accK:2*cagS:1*attL-gccaccacaacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcag:1*cagS*ttgV*actK*ttaE*tggF:1*aatE:2*gatQ:15*ctgI:1*aacK:1*tttM:2*ttaT:1*caaD*aagD*gctL:1*tcgE:1*cagE*gaaS*gccE*atcV-gagcgtcgtgagaagcttgag:1*gacK:3*aagS*aacA*gttL*gagS*ctgN*gcaV:1-ctg:2*gaaN*cgtN*gacN*agaK*gctG*agaE*ttgS*gacE*ttaR:1*aaaE*attA:1*gD~gt309ag-tc-ctgattgatcacctcact*aagR*ctcS*acaG*tcaM*ttgI*gttL*gatK*gccS*atcL*ttgV*gctE:1*ggtS:2*aggS*attL:2*tccP*ttgI*tacA*gagS:2-tctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaag*gccV*caaS*tcgD*tccL:3*tttL*gccS:1*tctA:1*aatI:1-ttcattgccgatggtccaaat:2*cacI*tctN*gagT:1*atcV*agaN*tccI*attL*aacP:2*acaV:1*tctL*atcL*gccL*gatR*gttC*ctgP*ctcQ*aatE*gccM*aagQ*ggtT:1~gt798ag*gatA*gttE:2*tcgL*aagR*ttgC*acgL*aacS-catgagaacaagactatg*gagS*atgA*gagK:1*caaT*gtgD*gagS*attV*ttgI*aagN*ttgA*gagN*aatI:1*ttgF*gctQ*tccE*gctK*agaL:7*aagQ:1*ggaS:2*gatY:5*gagV +BCGI01000001.1 miniprot mRNA 654447 657566 1076 + . ID=PF000019;Rank=1;Identity=0.3769;Positive=0.5276;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 671 +BCGI01000001.1 miniprot CDS 654447 655995 987 + 0 Parent=PF000019;Rank=1;Identity=0.4085;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 557 +BCGI01000001.1 miniprot CDS 656305 656606 21 + 2 Parent=PF000019;Rank=1;Identity=0.2000;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 558 624 +BCGI01000001.1 miniprot CDS 657405 657566 68 + 0 Parent=PF000019;Rank=1;Identity=0.3585;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 625 671 +BCGI01000001.1 miniprot stop_codon 657564 657566 0 + 0 Parent=PF000019;Rank=1 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 228 150 212 + BCGI01000001.1 3147266 644642 644828 102 186 0 AS:i:178 ms:i:178 np:i:45 fs:i:0 st:i:0 da:i:-1 do:i:3 cg:Z:62M cs:Z:*cgaQ:1*gcgC*cttY:1*gcaT*aagY:2*acaS*tcaF:1*accK*cctH:2*aagR*gagG*tttI:2*agaK*gtgI:2*gctV:1*aggQ*actC:2*agtT*acgS:2*aaaL*gttY:2*gaaQ:1*tgtL*ggaD:3*ctgS:3*gtaI:8*aacA:1*gaaK +BCGI01000001.1 miniprot mRNA 644643 644828 178 + . ID=PF000020;Rank=1;Identity=0.5484;Positive=0.7258;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 151 212 +BCGI01000001.1 miniprot CDS 644643 644828 178 + 0 Parent=PF000020;Rank=1;Identity=0.5484;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 151 212 +##PAF 4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 126 0 126 + BCGI01000001.1 3147266 591566 644825 147 393 0 AS:i:193 ms:i:221 np:i:78 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:7M7I9M6I34M5D7M52908U55M cs:Z:*tccM*tccD:1*ctcI:1*gtaS*ataM+AENINRT:3*cttI*tggL:2*tctE*cttI+DEINLN*cgcT:1*gaaN*tgtL*ggcY*aagN*ggcF:1*ttgM:1*acgS*accN*gacL:3*gatE*aagQ*atcH:1*atcL*agcH:1*gagN*cttI*gtgS*aagN*gaaN*accR*ataL*aacE*ctcI*aggY*gatH-cgtcgaagggccacc:1*gttI*catK:1*cacE*gacK*ggcS*tP~gt52905ag-ca:1*aagG*cgaK*acaS:2*accS:1*gaaQ*tccA*aagR*gagA:3*agaQ:2*aagR*gctR:1*aggQ*actS*ccaL:2*acgK:1*aggK*aaaE*gttE*ataV:1*gaaK:3*ctcI:9*acaI:5*aacS:1 +BCGI01000001.1 miniprot mRNA 591567 644825 221 + . ID=PF000021;Rank=1;Identity=0.3740;Positive=0.5954;Target=4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 1 126 +BCGI01000001.1 miniprot CDS 591567 591753 37 + 0 Parent=PF000021;Rank=1;Identity=0.2237;Target=4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 1 70 +BCGI01000001.1 miniprot CDS 644659 644825 184 + 2 Parent=PF000021;Rank=1;Identity=0.5818;Target=4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 71 126 +##PAF 1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1064 0 1064 + BCGI01000001.1 3147266 654431 657563 1803 3210 0 AS:i:2843 ms:i:2843 np:i:778 fs:i:0 st:i:0 da:i:-1 do:i:0 cg:Z:6M2D95M1D172M4I12M5I23M2D265M9I24M3I157M1I38M1I40M3I201M1D5M cs:Z:*cttM*gaaA*aagT*atgL:2-acaatc*tccA*gacE*tctA*gacE:1*attT*tcaV*tcgH*ttgI:4*tctN*ctcP*gacE:10*gcaQ:2*gtcI:4*cacN*aagH:1*tccG:1*cagA:1*ttcW:1*cagG*ctgM:1*ctgI*ctcQ:1*gttL:1*ggtN:4*ctcV:2*acaA:12*ggcP:1*gctV:1*aagR:2*atgQ*cgtQ:1*gctR*gatS:1*atcL:1*ggcS:1*ggaA:1*tcaG*gtcA:1*ggtA-gat:1*ttcR*aagH*ggcT:1*ggaA*agaK:2*aagY:3*gtcG*tatL:2*agaK:8*cgtP*ggaD:10*gtcI:2*gttR*accN*gtgI*caaD*aacD:1*gacN:3*gagQ:2*ctcV:3*actD:4*ctgI:1*gatN*cttF:4*tttI:2*atcL*tccK:1*gacG:1*aagP*tctN:2*aagR:4*gtgI:10*aagR:1*attT:1*tctT:4*attM:1*cgcA*tccT:1*ggaN*tctD:1*gagD:6*gaaS:2*gagY:3*gccQ:2*tacL:3*gctY:4*atcL*cgcK:4*ttgV:4*agaT:1*ccaS*gaaQ*acaD*gcaP:3*atcY*tccG*aaaE*gatN*gagP*agtE*gacE*gtgG:1*agtA+PALP*gaaK:2*gccI:1*agaE*caaV*actA:1*gtcL:2+GATTA:1*gttS*ccaT*gaaT*ccaD*atcL*tcgT*gaaP*cagE:1*actV*atgV*ggtD*aacF*ttcW*caaS*cagT:1*attQ*gcaG*tctQ:1*ccaQ-actggt*gttL*gatQ*ttcQ*tggQ*aacQ*accL:2*aacQ*gtgQ*tttY:1*gccE:2*cagR:3*agaA*gaaQ*gccR*caaE*gaaA*aacE*atgL*cttQ:3*caaM*cagL*gctH*gagL:2*cgtQ*attQ*tggQ:2*cagE:2*gctR*gctM:10*cagA:5*ttgY:1*aacR:2*gagD:2*gtcA:6*ttgI:1*gcaN*atgL:1*caaG:3*aacR:3*ttaM:1*cagE:3*caaR:3*accG:2*cagG:1*attL*gccQ*accQ*acaV*acaI*cagA*actN*gctT*actN*cagL:1*cttS*ggtQ:3*gagD*cttM*ttgI*aacR:5*ttgV*actN*ttaS*tggL:1*aatS:3*tctV:13*aacD*ctgK:1*aacK*aagE*tttL:1*aagL:1*cagK*caaV:10*cgtK:5*aagR:1*ttgI:1*tctA:2*gttI:3*ggtD:9*ttgY:2*gagD:1*attS*aagR:1*ggtN*aaaQ*gacK*agcD*gagV:1*gatE:3*ctcR:1*atcL:1*gacL*ttgT*acaE*ttgD:2*gatA:1*tctA:3*cagK*tccG*tcgA*aacD:2*tcgL*gtgL*tttI*gctS*tctK:1*aagN:2*atgL*gaaA:2*aagE*gcaE*aaaA:1*gagK*acaA*cagK*tctQ*cgtS*gctV:1+DNYANRSFD*aacD*ccaS:2*gaaK*cagL:2*aaaA:2*gaaD*gagD:4*gtgL:1*caaE:1*atgV:1*gatS+MEA:2*cagK:1*cttM*gccS*agaL*gtcN*cagH*ggaN:1*aacA:1*gagQ:1*attL*aacE:2*cttI:1*gcaE:2*attK*gatE:2*actS:2*acaN:1*ttgI*gttI:1*gccS*atcV:1*gctE:1*ggtS*atcA*aaaE:1*attV:1*gacE:2*tacF:1*ctgF*gacE:10*tcaT:7*atcV:3*caaS*tcgT:6*aacT:3*gacN:2*gccI:6*cacY:1*gagG:2*agaS*tccT*attA*aacT*gaaI*ttcL*acaS:1*tctV:1*gccP:3*ctcN:4*attL*gtgT:2*gtgA*aagR*actD:1*agtT*gaaV*tctA*aatD*cagN*ctcI:1*gccS*gccY*tccA:1*tctL*accA:1*cagV*gaaA*tctV:3*cttF*atgS*tctG*ctcV*ctcQ:1*gatN+K*atcL*cttA*gaaQ*ggaL*actS*gagD:1*gagD:2*gaaD*gttK:2*tccN*ggtF:1*ttgI*aacD:2*gagT*aagH:2*gaaS:1*attS*gttQ*attV*gctS:1*aacK:1*gcaT:2*tctA+G*gttT*ctcH*aagL:1*attG*aatS:5*gctL:2*aacR:1*ttgM*aacA*tctR:2*tctE:2*acaE:2*gctN:1*catK:1*aagN*gatE:2*aacS*tccR*tctP*ttcK:1+PKF:1*tccV*atcF:1*ggtL*gatK:1*aacH*aagE:3*tctA:3*tccA:2*aacT*tcaA:2*cttA:2*cagK:2*attS*gagD*acaC:1*aatQ:3*acgA*aaaQ*aatG*cagK:1*agtA*ggtS*accS:1*gccT*cagA:17*gctS:5*aagN*atcV:2*ggtE:7*tctG*aagN*aacH:1*catP:1*gaaQ:9*gctS:13*acaS*ttcY:6*acgR:2*aatG:3*actA:16*aacS*cagK*agaK*acgQ*aatQ*ggtK*ggtE*gatE*cagN:3*ttcY:4*aacP:1*gagV*aacL:2*atgT:13*gccS:1*gctN:12*ggaS:1*agaV:1-ggt:1*agtE:3 +BCGI01000001.1 miniprot mRNA 654432 657566 2843 + . ID=PF000022;Rank=1;Identity=0.5617;Positive=0.7271;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +BCGI01000001.1 miniprot CDS 654432 657566 2843 + 0 Parent=PF000022;Rank=1;Identity=0.5617;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +BCGI01000001.1 miniprot stop_codon 657564 657566 0 + 0 Parent=PF000022;Rank=1 +##PAF 4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1053 0 1046 + BCGI01000001.1 3147266 654440 657560 1644 3192 0 AS:i:2624 ms:i:2624 np:i:763 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:99M4I179M1I13M3I14M6D6M4I14M2I16M2D226M5I22M1D23M8D133M1I34M1D32M4I211M cs:Z::2*aagS*acaN*atcR*tccA*gacE*tctV:2*attN*tcaV*tcgN*ttgI:4*tctN*ctcT:2*actS:14*tggH:2*aagR:2*agaK*cagA:1*ttcW:1*cagG*ctgI*aaaR*ctgI*ctcQ:1*gttL:1*ggtT:1*gaaD:1*caaM*ctcV:2*acaA:12*ggcP:3*aagR*gagD:1*atgQ*cgtA:1*gctV*gatN*tggF:1*gagQ*ggcS:1*ggaA:1*tcaG*gtcN*catN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aagS:3*gtcR*tatF:1*ttaM*agaQ:2*aacT:3*aacY:1*cgtP*ggaA:3*acaM:4*gagD:1*gtcI:2*gttR:2*caaN*aacD:1*gacN:6*ctcM*gatN:6*gctG*ctgV:2*cttF:3*atcV:1*gctS:1*atcL*tccQ*catR*gacS:1*aagS*tctN:5*gctS:3*ttgM*gttI:7*aagR:1*attV*acgI:5*attM:1*cgcV:3*tctN:5*cctS:2*gaaQ:1*tatF*gagY:3*gccD:2*tacR*gagD:2*gctY:3*tcaT*atcL*cgcK:2*actR:1*ttgI*attV:3*agaQ:1*ccaG*gaaK*acaN*gcaP:3*atcL*tccE*aaaE*gatE*gagD*agtG*gacP*gtgG*gaaL*agtP*gaaQ:2*gccK:1+V*agaA*caaE:1*tcgR*gtcS*gctV*acaS:1*gttE:1*gaaA:1*atcL+PVA*tcgT*gaaP*cagT:1*actS*atgI*ggtP*aacA*ttcE*caaA:3*gcaE-tctcagccaactggtgtt*gatN:2*aacS*accQ*caaD+ALLA:1*aacQ*gtgQ*tttY*gaaD:3*cagE:2*gcaR*agaQ*gaaQ+QA*gccM*caaE:1*aacE*atgR*cttI*caaR:1*cagM:1*cagM*gctQ*gagQ:2*cgtQ-atttgg:2*cagM:2*gctR*gctQ:1*gctM:1*cagA:1*aggQ*ctaR:5*ttgM*cgcA:2*ttgM*cagA*aacR*cagH:1*gagQ:2*gtcM:6*ttgI:2*atgL:1*caaG:2*gagD*aacQ:3*ttaM:1*cagE:3*caaG:3*accA:2*cagA:1*attL*gccN*accQ*acaL*acaQ:3*actH:1*caaS*cttA*ggtQ*tctA:2*gagD:1*ttgI*aacE:3*gaaQ:1*ttgI:1*ttaM:1*aaaR*aatQ:2*gatE*tctT:3*ctgR:2*cagS*ttgM:1*cagE:1*catY:1*aacA:2*aacK:1*tttL:1*aagA*ttaT:7*cagK:4*cgtK*cgtA:4*aagR:1*ttgM*aagR*tctH:2*gttI:3*ggtD:2*agaK:6*ttgY:2*gagD*aaaR*attA:3*aaaN*gacK*agcE*gagD*attV*gatE*aagR:2*ctcR*cagE*atcL:1*gacM*ttgA*acaQ*ttgD:2*gatA:1*tctK*gagD:2*cagT*tccG*tcgA*aacD:2*tcgL*gtgL*tttL*gctS*tctK:1*aagN:2*atgL*gaaS*gacE:1*aagE*gcaN*aaaA:1*gagK*acaM*cagK*tctQ:3+DERGD*aacD*ccaS:2*gaaL*cagR*aagR:1*aaaE:3*gagI:1*cttY:1*attA*gtgL*cagN*caaE*accA*atgF-gat*gatN*gccS:1*cagA*gagL*cttH*gccQ*agaE*gtcQ:2*gagN:1*gatS*gagN*gctG*attG*aacS*gaaT*caaP:1*gatA:1-gtcctgattgatcacctcactaag:1*acaH:1*ttgI*gttI:2*atcL:1*gctE:2*atcS*aaaQ:1*attV:2*tccA:1*tacF:2*gacE:10*tcaT:6*atcV:4*caaS*tcgE:2*accS*gacA:2*aacT:3*gacN:1*attL:2*ggtE*ccaT*aatD:2*cacY*tctA:3*agaK*tccT*attV:1*gaaI*ttcY*acaS*tctT*tctA*atcV*gccE*gatN:2*ctcS:1*gccS:2*attL*gtgT:2*gtgA:1*actD:1*agtA*gaaS*tctA*aatD*cagA:1*attV*gccN*gccF*tccA:1*tctE*accS:1*cagE*gaaA*tctT:1*gacR*ttcN*cttF*atgI*tctG:3*gatE:1*cttI:1*ggaD*actF*gagP+L*gaaD*gagD:1*accM:1*gttR:2*tccT*ggtL:1*ttgM:3*gagT*aagA:2*gaaH:1*attT*gttK*attL:2*aacR*cttM:2*agaK*tctT*gttN*ctcI-aag:1*attM*aatS:5*gctL:1*gatE*aacR:1*ttgM*aacA*tctK:2*tctD:2*acaA*tctA:1*gctS:1*catK:1*aagG:3+KFNQ*aacS*tccD*tctP*ttcL*gatK:1*tccT*atcT:1*ggtL*gatQ*atcL*aacH*aagE:1*atcA*ttaI*tctQ:2*attQ*tccA:1*accI:1*tcaA:2*cttA:2*cagR:2*attT*gagD*acaA:5*acgA*aaaQ*aatG*cagR:1*agtT*ggtS*accS:7*cacN:1*agaK:10*gctS:5*aagN:3*ggtE*actK:3*gtgT:1*agtR*tctR*aagT*aacS*accG*catL:8*gaaN:24*acgK:2*aatE*gctC:2*actV:6*tctN:2*aacK:3*gatE:3*cagK*agaK*acgF*aatG*ggtE*ggtL:1*cagE*gatK:2*ttcY*tcgA*aagA:1*acgS*aacK:2*aacF:2*atgT:13*gccD:1*gctQ*tccG:6*gagQ*attM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gatE:1 +BCGI01000001.1 miniprot mRNA 654441 657560 2624 + . ID=PF000023;Rank=1;Identity=0.5150;Positive=0.7171;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +BCGI01000001.1 miniprot CDS 654441 657560 2624 + 0 Parent=PF000023;Rank=1;Identity=0.5150;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +##PAF 4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1054 0 1047 + BCGI01000001.1 3147266 654440 657560 1644 3195 0 AS:i:2613 ms:i:2613 np:i:763 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:99M4I179M1I13M3I14M6D6M4I14M2I16M2D226M5I22M1D23M8D133M1I34M1D25M1I7M4I211M cs:Z::2*aagS*acaN*atcR*tccA*gacE*tctV:2*attN*tcaV*tcgN*ttgI:4*tctN*ctcT:2*actS:14*tggH:2*aagR:2*agaK*cagA:1*ttcW:1*cagG*ctgI*aaaR*ctgI*ctcQ:1*gttL:1*ggtT:1*gaaD:1*caaM*ctcV:2*acaA:12*ggcP:3*aagR*gagD:1*atgQ*cgtA:1*gctV*gatN*tggF:1*gagQ*ggcS:1*ggaA:1*tcaG*gtcN*catN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aagS:3*gtcR*tatF:1*ttaM*agaQ:2*aacT:3*aacY:1*cgtP*ggaA:3*acaM:4*gagD:1*gtcI:2*gttR:2*caaN*aacD:1*gacN:6*ctcM*gatN:6*gctG*ctgV:2*cttF:3*atcV:1*gctS:1*atcL*tccQ*catR*gacS:1*aagS*tctN:5*gctS:3*ttgM*gttI:7*aagR:1*attV*acgI:5*attM:1*cgcV:3*tctN:5*cctS:2*gaaQ:1*tatF*gagY:3*gccD:2*tacR*gagD:2*gctY:3*tcaT*atcL*cgcK:2*actR:1*ttgI*attV:3*agaQ:1*ccaG*gaaK*acaN*gcaP:3*atcL*tccE*aaaE*gatE*gagD*agtG*gacP*gtgG*gaaL*agtP*gaaQ:2*gccK:1+V*agaA*caaE:1*tcgR*gtcS*gctV*acaS:1*gttE:1*gaaA:1*atcL+PVA*tcgT*gaaP*cagT:1*actS*atgI*ggtP*aacA*ttcE*caaA:3*gcaE-tctcagccaactggtgtt*gatN:2*aacS*accQ*caaD+ALLA:1*aacQ*gtgQ*tttY*gaaD:3*cagE:2*gcaR*agaQ*gaaQ+QA*gccM*caaE:1*aacE*atgR*cttI*caaR:1*cagM:1*cagM*gctQ*gagQ:2*cgtQ-atttgg:2*cagM:2*gctR*gctQ:1*gctM:1*cagA:1*aggQ*ctaR:5*ttgM*cgcA:2*ttgM*cagA*aacR*cagH:1*gagQ:2*gtcM:6*ttgI:2*atgL:1*caaG:2*gagD*aacQ:3*ttaM:1*cagE:3*caaG:3*accA:2*cagA:1*attL*gccN*accQ*acaL*acaQ:3*actH:1*caaS*cttA*ggtQ*tctA:2*gagD:1*ttgI*aacE:3*gaaQ:1*ttgI:1*ttaM:1*aaaR*aatQ:2*gatE*tctT:3*ctgR:2*cagS*ttgM:1*cagE:1*catY:1*aacA:2*aacK:1*tttL:1*aagA*ttaT:7*cagK:4*cgtK*cgtA:2*cttM:1*aagR:1*ttgM*aagR*tctH:2*gttI:3*ggtD:2*agaK:6*ttgY:2*gagD*aaaR*attA:3*aaaN*gacK*agcE*gagD*attV*gatE*aagR:2*ctcR*cagE*atcL:1*gacM*ttgA*acaQ*ttgD:2*gatA:1*tctK*gagD:2*cagT*tccG*tcgA*aacD:2*tcgL*gtgL*tttL*gctS*tctK:1*aagN:2*atgL*gaaS*gacE:1*aagE*gcaN*aaaA:1*gagK*acaM*cagK*tctQ:3+DERGD*aacD*ccaS:2*gaaL*cagR*aagR:1*aaaE:3*gagI:1*cttY:1*attA*gtgL*cagN*caaE*accA*atgF-gat*gatN*gccS:1*cagA*gagL*cttH*gccQ*agaE*gtcQ:2*gagN:1*gatS*gagN*gctG*attG*aacS*gaaT*caaP:1*gatA:1-gtcctgattgatcacctcactaag:1*acaH:1*ttgI*gttI:2*atcL:1*gctE:2*atcS*aaaQ:1*attV:2*tccA:1*tacF:2*gacE:10*tcaT:6*atcV:4*caaS*tcgE:2*accS*gacA:2*aacT:3*gacN:1*attL:2*ggtE*ccaT*aatD:2*cacY*tctA:3*agaK*tccT*attV:1*gaaI*ttcY*acaS*tctT*tctA*atcV*gccE*gatN:2*ctcS:1*gccS:2*attL*gtgT:2*gtgA:1*actD:1*agtA*gaaS*tctA*aatD*cagA:1*attV*gccN*gccF*tccA:1*tctE*accS:1*cagE*gaaA*tctT:1*gacR*ttcN*cttF*atgI*tctG:3*gatE:1*cttI:1*ggaD*actF*gagP+L*gaaD*gagD:1*accM:1*gttR:2*tccT*ggtL:1*ttgM:3*gagT*aagA:2*gaaH:1*attT*gttK*attL:2*aacR*cttM:2*agaK*tctT*gttN*ctcI-aag:1*attM*aatS:5*gctL:1*gatE*aacR:1*ttgM*aacA*tctK:2*tctD:2*acaA*tctA:2+S:1*catK:1*aagG:3+KFNQ*aacS*tccD*tctP*ttcL*gatK:1*tccT*atcT:1*ggtL*gatQ*atcL*aacH*aagE:1*atcA*ttaI*tctQ:2*attQ*tccA:1*accI:1*tcaA:2*cttA:2*cagR:2*attT*gagD*acaA:5*acgA*aaaQ*aatG*cagR:1*agtT*ggtS*accS:7*cacN:1*agaK:10*gctS:5*aagN:3*ggtE*actK:3*gtgT:1*agtR*tctR*aagT*aacS*accG*catL:8*gaaN:24*acgK:2*aatE*gctC:2*actV:6*tctN:2*aacK:3*gatE:3*cagK*agaK*acgF*aatG*ggtE*ggtL:1*cagE*gatK:2*ttcY*tcgA*aagA:1*acgS*aacK:2*aacF:2*atgT:13*gccD:1*gctQ*tccG:6*gagQ*attM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gatE:1 +BCGI01000001.1 miniprot mRNA 654441 657560 2613 + . ID=PF000024;Rank=1;Identity=0.5146;Positive=0.7164;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +BCGI01000001.1 miniprot CDS 654441 657560 2613 + 0 Parent=PF000024;Rank=1;Identity=0.5146;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 diff --git a/results/2026-10-30_petasospora/data/GCA_001983305.1_ASM198330v1.miniprot.gff b/results/2026-10-30_petasospora/data/GCA_001983305.1_ASM198330v1.miniprot.gff new file mode 100644 index 00000000..a7a75639 --- /dev/null +++ b/results/2026-10-30_petasospora/data/GCA_001983305.1_ASM198330v1.miniprot.gff @@ -0,0 +1,72 @@ +##gff-version 3 +##PAF MH260578_SUI1 109 0 109 - MPUK01000005.1 1064489 593641 593968 267 327 0 AS:i:488 ms:i:488 np:i:103 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:109M cs:Z::2*tctN:1*cagE:2*aacK*actS:10*gatE:1*cagE*ccaV:30*agaK:2*cgtK:1*ttgI:2*gacE:24*gtcL:1*gttI:3*ttgM*accV*tccQ*cagT:1*gccG*ttgI*cagK:9 +MPUK01000005.1 miniprot mRNA 593639 593968 488 - . ID=PF000001;Rank=1;Identity=0.8165;Positive=0.9450;Target=MH260578_SUI1 1 109 +MPUK01000005.1 miniprot CDS 593639 593968 488 - 0 Parent=PF000001;Rank=1;Identity=0.8165;Target=MH260578_SUI1 1 109 +MPUK01000005.1 miniprot stop_codon 593639 593641 0 - 0 Parent=PF000001;Rank=1 +##PAF MH260578_SLA2 1072 29 1072 + MPUK01000005.1 1064489 627815 630917 1701 3156 0 AS:i:2748 ms:i:2748 np:i:773 fs:i:0 st:i:0 da:i:21 do:i:0 cg:Z:94M2D73M1D86M1I24M3I24M2I256M5I54M4D129M1I41M3I39M3I150M2D55M cs:Z:*tctT:1*cttI*attS*tcaT*tcgA*ttgI:3*actC*tctT*ctcP*gacE*gaaD:2*ccaS:18*agaK*cagD:1*ttcW*aacE*cagT*ctgM:1*ctgV*ctcV:1*gttL*cagK*ggtN*gatT*gaaD*gttT:4*acaA:2*accL:9*ggcP:3*aagV*gagG*gccG*atgF*cgtK:1*gctM*gatE:2*gagS*ggcN:1*ggaD*agaT*tcaL*gtcR*catT*ggtL:1-ggattc*aagR*ggcR*tacF*ggaQ*agaV:2*aagP:3*gtcN:1*ttgI:1*agaQ:2*aacR:2*aagH:17*gttR:2*caaS*aacD:1*gacN:3*gagD:5*atgT*actS:3*gctS:2*gatS*cttF*caaG:1*ctgD:3*agtT*atcV-tcc*catR*gacN*agaN*aagN:3*aagI:6*ttgI:10*attL*acgI:5*attM*cacY*cgcK*tccM*acgS*ggaD:1*gatA:1*gcaI:4*agaK*gaaS*cgcK*tatF:1*tctD:2*gccE:3*gagD:3*gacN:2*tcaN:5*tccT:5*agaK:2*gaaF*acaE:4+I:1*tccQ*aaaN:2*agtD*gacE*gtgN*gaaV*agtN*gaaL*agaP:1*gccL*tcaT*agaE:1*actK*tcgT*gtcA:2*ccaT*gttL+RHS:1*gaaT:1*atcF*tcaE*gaaQ*cagE*ccaN*actE*atgR*ggtQ*aacQ*ttcQ:1*cagA:3*tctT:4*gttM+VR*gatN*ttcA*tggF*aacV*accE:2*aacQ*gtgL*tttY:1*gccQ*gaaQ:2*agaQ:1*gcaE*agaQ*gaaQ*gccR:1*gaaA*aacE*atgL*cttQ:5*gctQ*gagL*caaA:1*cgtQ*attQ*tggY*tttW:4*gctR*gctQ:3*cagA:1*aggQ:3*gaaQ:3*cgcL:2*ttgA:1*aacR*cagH:1*gagQ:9*ttgI:1*gcaT*atgL*aggK*caaG:2*gagD*accN:3*ttaM:2*cagS:3*agaK:1*aagQ*accA:2*cagN:1*attL:1*accS:5*actQ*cagE:2*ggtA:4*cttQ:2*agtL:4*ttgV*actE*ttaY:5*gatE:10*cagT:6*aacT:1*tttS:2*ttaI:12*cgtK:5*aagR:1*ttgM:1*tctA:2*gttI:3*ggtD:2*agaK:7*gacE:2*aagR*attF:1*ggtN:1*aaaT*gacK*agcT*gagD*attL*gatQ*aagT:1*gagQ:1*cagE*atcK*aggD*gacE:1*acaE*ttgQ:2*gatA*gacT*tctL*gagD:1*agcA:2*tcgA:2*tcgT*tcgA*gtgI:1*gctN*tctQ:1*aagN*agaK:1*atgL*gaaQ*gacE:1*aagQ*gcaN:1+FKDVT:1*gagN*acaA*cagP:1*cgtD*gctE*ctgK*aacL*ccaK*gacQ:2*cagE*aagQ:1*aaaQ:2*gaaD*gagL:1*cttI:1*attM*gtgM:5*gatE*gccT*cttI*cagK*gagD:1*gccV*agaK*gtcT:1*ggaN*gagS*aacR*gatE:1*gctK*attS*aacE*gaaM:1*cttN*gatK*gcaL-gtcctgattgat*cacN*ctcS:2*ctcV*accL*tcaG:1*gttI:4*gctK:3*aaaN:4*tccA*ttgV*tacF:10*caaL:6*ttgV:1*tccT*atcL*atcL:2*gccS*caaS*tcgG*tccL*gctV:4*aacS:3*gacN*ttcM*attL*gccV:2*ccaD:1*ggtA*gatN*cacE*tctA*gagL*atcV:1*agaD:2*aacT*gaaN:2*tctT:2*gccS:1*gttI:3*gccT:2*attL*gtgT:2*gtgT:1*actQ:1*agtD*gaaF*tctQ*aatD*cagD:2*gccD*gccT*tccA*agaK*tctD*accI:1*cagE*gaaI:2*gacV*ttcY:1*atgE:2*ctcY+H:1*gatN*atcL*cttV*gaaN*ggaK*actS*gagI*gaaD*gagD*aaaQ:1*gaaD*gttI:2*tccN:2*ttgI*aacD:3*aagI:2*gaaT:1*attL*gttQ*attL*gctV:1*aacS*cttM*gcaK*ccaT*agaP:1*gttS+KID:1*aagS*aacK*attV*aatD:1*gatE:1*ggtS:1*gctL:2*aacK:1*ttgM*aacE*tctH:1*gctS*tctR*gctV:1*acaE*tctE:1*gctS*gctV:2*aagS*gatS:3*tccQ*tctP:2+VSM:4*ggtI*gatE*atcV:2*gccS:2*tctA:2*attS*tccA*gttI*accI*aacT*tcaA:1*gctK*cttY:2*cagR:1*gccS:2*acaS:1*aatE:3*acgN*aaaN*aatG*cagR:2*ggtH*accS*cgtK*gccT*cagS:4*cacN:1*agaK:8*gctS:1*gctS*gttI:1*gccY:2*aagN*atcT:2*ggtR*actI:5*agtQ*tctN:1*aacH*accS*catN:3*attV:3*aatR:10*gctS:1*tcaA:3*gctS*acaQ:6*gatN*acgK:2*aatD:3*actK:1*actN*tctL:2*agaK*tctQ:2*aacD:2*cagN*gatK*attL*ctgI*aacI*cagG*agaK*acgH*aatE-ggtggt*gatV*cagE:3*ttcY:3*acgS*aacI:5*atgA:15*gctS:11*tatF*ggaS:1*agaK:1*ggtD:5 +MPUK01000005.1 miniprot mRNA 627816 630920 2748 + . ID=PF000002;Rank=1;Identity=0.5390;Positive=0.7348;Target=MH260578_SLA2 30 1072 +MPUK01000005.1 miniprot CDS 627816 630920 2748 + 0 Parent=PF000002;Rank=1;Identity=0.5390;Target=MH260578_SLA2 30 1072 +MPUK01000005.1 miniprot stop_codon 630918 630920 0 + 0 Parent=PF000002;Rank=1 +##PAF MH260578_TGL1 440 12 440 - MPUK01000004.1 1227680 905467 906742 585 1314 0 AS:i:911 ms:i:911 np:i:269 fs:i:0 st:i:0 da:i:27 do:i:51 cg:Z:15M2I11M2D4M5D23M1D51M4I229M7I20M1D29M1D33M cs:Z:*tcgA*ctcV:1*gagQ*tggL:2*gtcL*gtgI*gccF*tcgH*ttcK:1*gctG:1+EE:2*gagS*tttY*gtaF*atcQ*tccT*cttP*atcS*accN:1-gccatc:2*tcaN*gtgI-ctcgacctcgttaca:1*ttcA*atcV*caaS:1*gtgE*cacN*tctK:1*atcK*gttI*ccaS*gaaP*aagS*atcA:1*aacP*ggtL:1*ataE*ctcV*gagA:1-gct*gccT*aagH*aagC*atcL*acaH*aagQ*gatK:1*aggI:2*cctN*tcaD:1*caaV*cacD:1*ggaV:1*ctcA*tttH:2*caaK*attV*gatH*gatE:3*cctQ:1*gcaK:5*tgtS*cttI:3*aggL*ccaK*ctcS*aagS*gaaG*ggaD*gcaK:1+QNSR:9*atgT*tgcN:1*gatE*atcL*tggF*gttL*gtcL*aacG*atcE*aagN:2*agaK*aatC:3*attL:1*catV:1*atgQ:1*tttY:3*atgL:9*acaR:3*tccF:1*aaaS*ccaS*tcaT*catD*aagE*agaK:2*gacN*ttcY:1*atcL:5*tatF:3*gatN*tccT*atcV:4*tcaR*tacN:1*aagQ:1*caaD*gacS:1*acaS:10*gcaC*tttL:5*catN*gatH*aaaD:2*accK*cagK:1*aagN*ctcK:2*gctG:1*gctS:3*acaI:1*agtQ:2*cacN:1*caaP*ttcI*gtcA*gatS*acaF:1*atgV*aaaT:1*acaA:1*aacG*ttcL*atgL:1*ctgR*gccI:4*ctcA*ctcI:2*acaS*gcaV*tcaV:2*aaaQ*aatK*atcL*tgtM*tacG:1*ccaV:1*ttcY*tctE:1*atcV*atcV:1*cttK:1*gttL*cgcV*atgF:2*gacN:2*aatS*tacG:2*actK*catT*gatS:2*attK*gctV*gcaG*ttcY*tccP:1*ctcM*tacF:2*acaS:5*gttA:6*cgcD*aatS*ggtK*gcaR:2*ttgM:3*gacG+GASGSRL:1*tcaY:1*accS*aatG*gccT*ctcS*tctG*gctK*ccaT*ttcN:1*aacV:7-aat:1*aaaT*gtaT:1*atcC*aagL*ttgF*attV:2*tcaK*attS:1*tccM:1*gtgI:2*gatE*aagR*atgT:1*tcaD*ttaN:1*ccaS*gctC:2-aca*gatQ*gctT*gtaI*gcaG:1*ccaD*aatG*catY:2*ttgM:1*atcT*atcL:1*ggtA:1*cacQ*attV:2*ctcK:3*catV*ttgV*ttcL*aatQ*ttcY:1*ggtK*gtcQ +MPUK01000004.1 miniprot mRNA 905468 906742 911 - . ID=PF000003;Rank=1;Identity=0.4452;Positive=0.6142;Target=MH260578_TGL1 13 440 +MPUK01000004.1 miniprot CDS 905468 906742 911 - 0 Parent=PF000003;Rank=1;Identity=0.4452;Target=MH260578_TGL1 13 440 +##PAF MH260578_TGL1 440 79 440 + MPUK01000012.1 301241 188568 189630 525 1101 0 AS:i:877 ms:i:877 np:i:236 fs:i:0 st:i:0 da:i:54 do:i:45 cg:Z:90M1I91M1I86M11I10M3D34M3D37M cs:Z::1*tttI*gcaV*tctD*cttI*gctV*gcaH:1*caaH:2*cagK:1*caaH:2*actI:3*tccK:5*actS*ctcI:2*gtcI*tttL*gaaK*aagS*gaaS*gttG*tccD*tgcK*cagP*aagQ*ggcN*aagS*cctR:1*gctV:1*ttcL:11*atcL:2*gacE*cgcN*cgtK*gatE:4*tatF:2*ttgV*gagD*aagQ:15+R*tccK:1*attL*acaF:1*actS*gagS*tctT:1*ccaE*tgcK*tacF:1*gacN*ttcY:6*cacM*acaF:3*tctN*attT*atcV:4*gacR*ttcN:1*ggcQ:1*ccaD:2*actS:7*acaS*acaA:1*atgC*tttL:2*atgL:1*attL:1*gagH:3*tctK:1*gtcI:1*ttcK:1*gttI*gctG:3*gtgA:4*ccaG:1*actN*cacN:1*ctaI*atcA*aagS*ccaF*ttcL:1+T*caaS*actA:1*tcaG*aatL*atcL*accY*aagR*gtgI:2*agcK*ggaR*cctA*ttaI:2*ttcS:1*aatV*cttF*gtcW*aatQ:1*gccL*atcM*ccgG*cttP*cctV:2*aagE*accK*atcV*attV:1*gtcK:2*ggtV*atcF:3*accW*tcaK*ggaS*gcaG:2*agcK*tcgT*aagS:2*ctcK*gcaV:3*tacH*ttgM:2*gttP:4*tgtS:1*gtaA:6*agaD*cagS*caaK*tccR:1*cacQ*aagM*tacF+DEGGASGSRLV:1*aaaL*gctS*gctG*gttT:1*aaaG*atgK:1*gatN-gacctctgg*gagR:1*gagA*ctcS:1*gatP*atgT*aaaQ*aatT*ctgI*aaaT*gtgT:3*ttgF:3*gacK*gccS:1*atcM:1*gtcI:2*gatE*aagR:1*aggL*cagD*catN*ttcL-aagcagtca*aacS*ctgC:5*aagG*gtgI*agtD:11*gagQ:1*ttgE*tatK*acgK:2*gcaP*cctV:2*gagQ*aagY*attL*gaaK*gagQ +MPUK01000012.1 miniprot mRNA 188569 189630 877 + . ID=PF000004;Rank=2;Identity=0.4768;Positive=0.6431;Target=MH260578_TGL1 80 440 +MPUK01000012.1 miniprot CDS 188569 189630 877 + 0 Parent=PF000004;Rank=2;Identity=0.4768;Target=MH260578_TGL1 80 440 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1019 7 1019 + MPUK01000005.1 1064489 627809 630908 1557 3129 0 AS:i:2420 ms:i:2420 np:i:724 fs:i:0 st:i:0 da:i:15 do:i:9 cg:Z:256M2D10M1D15M2D10M4D5M2I31M1I8M1D218M1I8M7D22M4D11M7D70M1I34M1D30M1I40M1I26M1I11M2I140M2D57M cs:Z::1*gacN*tctL:1*cttI*attQ*tcaA:5*actC*tctT*ctcA:1*gaaA*actA:21*cagE:1*ttcW*aacH*cagC:5*gttI:1*ggtS*gatN*gaaD*gttT:1*ctcI:5*accV:9*ggcP*tctT*gctC:1*aagI*gagG*gccG*atgY*cgtK:1*gctI*gatS:1*atcL:1*ggcS:1*ggaS:1*tcaF*gtcS*catN*ggtN*gatG*ggaT*ttcA*aagA:2*ggaT:7*gtcF:2*ttaE*agaQ:2*aacK:2*aagH*aacD:6*acaM:9*gttR:2*caaS*aacD:1*gacN:2*tacF:1*gccS:1*ctcM:2*atgL*actS:3*gctS:2*gatN:3*ctgV:2*gctS:2*tccR:1*gacT*agaS*aagE:3*aagV:2*gctS:3*ttgI*gttI:9*attL*acgI:3*aggK:1*attL*cacY*cgcK:1*acgS*ggaE:3*gcaV*ttgI*gaaA:2*agaK*gaaD:1*tatF*gagD*tctV:2*gccR:2*tacF:9*cgcK:3*tccT:1*attV:6*gaaS*acaS*gcaP:1-aacctc:1*tccL*aaaQ:2*agtE:1*gtgP*gaaQ*agtG-gaa*agaF:1*gccL*tcaV:1*caaE*actR:1*gtcS*gctV*acaS*ccaN*gttS*ccaE:1-ccaatc:1*gaaN*cagI:1*actR*atgS*ggtS:1*ttcI:1-cagccaattgca:5+AM:2*ttcS*tggF*aacV*accR:2*aacQ*gtgE*tttL:1*gccE*gaaQ:1*cagR*agaQ*cttA*gcaE*agaE*gaaR*gccR:1*gaaA*aacE*atgL:1*caaE:2*caaM+L:1*gctR*gagD:1*cagA:1*attF:1-ttt:1*cagE:2*gctR*gctQ:2*gaaV*cagS:1*aggE:3*gaaS:3*cgcA:2*ttgM:1*aacR:2*gagQ:1*agaK*gtcL:1*gagQ:2*cgtQ:3*gcaT*atgL*aggG*caaG:1*tacH:1*accR:3*ttaM:1*cagE:7*accV:2*cagN:1*attL*gccR*accI*acaV:4*actE*cagE:2*ggtA*tctV:1*gatT*gagS*cttQ*ttgC*aacS*agtH:1*cagE:2*ttgV*actQ*ttaY:5*gatE:17*aacG:3*aagI*ttaV:9*gccS:1*gagD*cgtK:9*tctA:2*gttI:3*ggtD:2*agaK:9*gagA*aagR:3*ggtN*aaaD*gacS*agcE*gagQ*attV*gatQ*aagM:1*gagR*ctcT:1*atcL:2*ttgM*acaE*ttgT:2*gatK*gacS*tctL:1*agaE*agcT*cagH:1*tcgQ:2*tcgA:1*gtgL:1*gctS*tctQ:1*aagS*agaK:1*atgI:1*gacE*ttgI:2*aaaS*cttA+S:1*acaL*cagK*tctE*cgtG*gctG:2-ccagaccttgaacagaagctc*aaaE:1*aagA*gaaQ*gagQ:1*cttV*gagQ*attM*gtgL:2*accA:3*gccT*cttM:4-agagtccaggga:1*aacQ*gatH*gagQ:2*aacE*gaaK*caaE*cttA*gatE-gcagtcctgattgatcacctc:1*aagR*ctcV*accL*tcaR:1*gttI:1*gccT*atcL:1*gctK*tccV*ggtS*atcS*aaaD:1*attL*cagR*gacE*tccF*ttgV*tacF*gagN:1*gacE:2*atgV*cagY*gctS*ggtN*aatT*caaD*aacI*tcaP*tcaT*ccgA:3*cttG*tccT*atcM*atcL:1*aagR*gccV*caaS*tcgH*tccL*gctS:1*gacQ:1*gccS*aacT*tctT*ttcM*aatT:1*ttcY:3*ggtN*ccaM*aatK:1*gatE+S*cacE:1*gagG*atcL:1*agaK*tccA:1*aacI:1*ttcL:1*tctD:2*gccG*gatN*gttL*ctgF*ctcS:1*gccV:1*ggtA*attV*gtgL:1*cttD*gtgR:1*actD:1*agtV*gaaQ-tct*aatE*cagI:1*attV*gccE*gccG:1*agaK*tctD*accL:1*cagH*gaaM*tctV:1*gacL:1*cttF*atgE:1*ctcM:2*gatN*atcH:1*gaaL*ggaK*actL:1+I*gaaH:1*aaaR:1*gaaD*gttR:2*tccT:2*ttgI:4*aagV*cttI:1*gaaS:1*attM*gttQ*attV*gctV:1*aacV*cttF*gcaR*ccaD*agaP*tctG*gttR*ctcK*aagT*aacP*attV*aatS*ggtS:1+E:2*gatY*gctF*gttL:1*aacK:2*aacA*tctN*gccT*gctQ*tctA:1*atcV*acaE*tctK:2*gctG*catL:1*aagE*gatS*ctgM+S*cttT*aacR*tccH*tctK*ttcL*gatE*tcaV*tccQ*atcE:2+SL*gatE*atcV:1*aagE:2*ttaI:1*gctC:1*attS*tccA*gttI*accI:1*tcaA*atcV*gctK:2*attL*cagE:1*gccC:1*gagD*acaA:1*aatE:3*acgS*aaaR*aatG*cagR:2*ggtM*accS:2*cagL:4*cacN:10*gctS:1*gctQ:2*gccY*tccA:1*aagG:3*ggtR*actM:5*agtA*tctG*aagT:1*accS*catS:11*gctS*tccA:5*gctS*gccS:4*gctS*acaD*ttcL:2*aagQ*acgS*cagH*gatL*acgN:2*aatE:2*aagR*actK:3*gccS:1*agaK*tctM:2*aacT*caaK:1*cagR*gatS*attL:1-aaccag*agaL*acgS*aatD*ggtE*ggtS*gatN*cagK*gatT*attV:1*ttcY:1*aagQ:2*aacA:3*aagR:1*atgA:1*atgL:10*aatS:2*gctS*tccH:10*tatF*ggaS:3*ggtE*gacE:1 +MPUK01000005.1 miniprot mRNA 627810 630908 2420 + . ID=PF000005;Rank=1;Identity=0.4976;Positive=0.6942;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +MPUK01000005.1 miniprot CDS 627810 630908 2420 + 0 Parent=PF000005;Rank=1;Identity=0.4976;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 294 0 294 - MPUK01000005.1 1064489 611409 612297 525 894 0 AS:i:884 ms:i:884 np:i:221 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:9M4D89M1I99M1I95M cs:Z:*agcM*tccN:1*ccaD*tcaK:2*acaK:1-cacaaagtcgac*gtgI*acaA:1*cgcS*cagA*aagN*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgK*atcV:1*aaaG*ccaD*actS:1*ttcV*caaS*atgL:1*aagF*tctK:1*ttgI*gttK*catT*gatE:1*ctcV:1*ggtA*gttA:1*tctA:2*tctT:1*ggtS:8*ctcT*acaA:6*tatK:3*aatI*gttM*gttT+D:1*gctT*gatR*aagG:1*tcaA:1*atgT*gcaF*tacQ:2*cctA*gttA:2*tttI*tctA:1*ggtA*attV:9*atcV:8*acaN*ggtS:1*gccP*ccaE*gatS:3*aacH:4*atcL:2*gtcL*atcL*agaK*gtgI*gctT:3*ggaN*actL*tctT:7*actP:2*gtgA:2*gtgI:3*tcgA:4*actS:5*gacK*tggL:1*ataV+E*tccN*ttcL*ggtS:1*aacD*aagP*gacK*tctT*attK*tccA:1*ttcH:5*cttI*tcaA:6*attV:18*gagQ:1*gtcS*gttF*cacG:2*accK*accD:1*ttcI*aaaS*aacR:2*cctL*tctG:6*gttT:1*agtA:1*gttI:4*aacH:2*atcL:2*ttgI*gttA*gttL:1*cagE:1*aagR*cacR:1*cgtK*gttI:1*atgL +MPUK01000005.1 miniprot mRNA 611407 612297 884 - . ID=PF000006;Rank=1;Identity=0.5872;Positive=0.7416;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +MPUK01000005.1 miniprot CDS 611407 612297 884 - 0 Parent=PF000006;Rank=1;Identity=0.5872;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +MPUK01000005.1 miniprot stop_codon 611407 611409 0 - 0 Parent=PF000006;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 305 0 305 - MPUK01000005.1 1064489 611409 612315 516 915 0 AS:i:860 ms:i:854 np:i:224 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ctcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*acaP*ccaA*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*caaT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:2*tttL*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*actS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggaE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:4*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aacH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +MPUK01000005.1 miniprot mRNA 611407 612315 854 - . ID=PF000007;Rank=1;Identity=0.5639;Positive=0.7344;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 1 305 +MPUK01000005.1 miniprot CDS 611407 612315 854 - 0 Parent=PF000007;Rank=1;Identity=0.5639;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 1 305 +MPUK01000005.1 miniprot stop_codon 611407 611409 0 - 0 Parent=PF000007;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1045 0 1045 + MPUK01000005.1 1064489 627800 630917 1821 3156 0 AS:i:2981 ms:i:2981 np:i:800 fs:i:0 st:i:0 da:i:6 do:i:0 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D51M3D4M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tctA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tccN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcaE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*accR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aagR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ttgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*attV:1*ggtD*gatN*cttV:1*gatE*gctL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:6*tccA:2*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*acgN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gccY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE:2*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH-gatggtgac*agtA:3 +MPUK01000005.1 miniprot mRNA 627801 630920 2981 + . ID=PF000008;Rank=1;Identity=0.5770;Positive=0.7605;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +MPUK01000005.1 miniprot CDS 627801 630920 2981 + 0 Parent=PF000008;Rank=1;Identity=0.5770;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +MPUK01000005.1 miniprot stop_codon 630918 630920 0 + 0 Parent=PF000008;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1044 0 1044 + MPUK01000005.1 1064489 627800 630905 1812 3144 0 AS:i:2977 ms:i:2977 np:i:797 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D54M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tctA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tccN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcaE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*accR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aagR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ttgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*attV:1*ggtD*gatN*cttV:1*gatE*gctL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:6*tccA:2*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*acgN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gccY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE:2*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +MPUK01000005.1 miniprot mRNA 627801 630905 2977 + . ID=PF000009;Rank=1;Identity=0.5763;Positive=0.7605;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +MPUK01000005.1 miniprot CDS 627801 630905 2977 + 0 Parent=PF000009;Rank=1;Identity=0.5763;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 305 0 305 - MPUK01000005.1 1064489 611409 612315 516 915 0 AS:i:860 ms:i:854 np:i:224 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ctcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*acaP*ccaA*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*caaT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:2*tttL*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*actS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggaE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:4*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aacH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +MPUK01000005.1 miniprot mRNA 611407 612315 854 - . ID=PF000010;Rank=1;Identity=0.5639;Positive=0.7344;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 1 305 +MPUK01000005.1 miniprot CDS 611407 612315 854 - 0 Parent=PF000010;Rank=1;Identity=0.5639;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 1 305 +MPUK01000005.1 miniprot stop_codon 611407 611409 0 - 0 Parent=PF000010;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 305 0 305 - MPUK01000005.1 1064489 611409 612315 516 915 0 AS:i:860 ms:i:854 np:i:224 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ctcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*acaP*ccaA*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*caaT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:2*tttL*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*actS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggaE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:4*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aacH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +MPUK01000005.1 miniprot mRNA 611407 612315 854 - . ID=PF000011;Rank=1;Identity=0.5639;Positive=0.7344;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 1 305 +MPUK01000005.1 miniprot CDS 611407 612315 854 - 0 Parent=PF000011;Rank=1;Identity=0.5639;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 1 305 +MPUK01000005.1 miniprot stop_codon 611407 611409 0 - 0 Parent=PF000011;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1044 0 1044 + MPUK01000005.1 1064489 627800 630905 1812 3144 0 AS:i:2977 ms:i:2977 np:i:797 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D54M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tctA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tccN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcaE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*accR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aagR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ttgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*attV:1*ggtD*gatN*cttV:1*gatE*gctL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:6*tccA:2*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*acgN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gccY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE:2*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +MPUK01000005.1 miniprot mRNA 627801 630905 2977 + . ID=PF000012;Rank=1;Identity=0.5763;Positive=0.7605;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +MPUK01000005.1 miniprot CDS 627801 630905 2977 + 0 Parent=PF000012;Rank=1;Identity=0.5763;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 287 20 287 - MPUK01000005.1 1064489 611409 612204 492 801 0 AS:i:835 ms:i:835 np:i:206 fs:i:0 st:i:0 da:i:129 do:i:0 cg:Z:71M1I99M1I95M cs:Z::18*ccgK*atcV:1*aaaG*ccaD*actS:1*ttcV*caaS*atgL:1*aagY*tctK:1*ttgV*gttK*catT*gatE:1*ctcV:1*ggtA*gttA:1*tctA:4*ggtS:8*ctcT*acaA:6*tatK:2*gagG*aatI*gttM*gttT+D:1*gctT*gatK*aagG:1*tcaA:1*atgT*gcaF*tacQ:2*cctA*gttA:2*tttI*tctA:1*ggtA*attV:9*atcV:8*acaN*ggtS:1*gccP*ccaE*gatS:3*aacH:4*atcL:2*gtcL*atcL*agaK*gtgI*gctT:3*ggaN*actI*tctT:7*actP:2*gtgA:2*gtgI:3*tcgA:4*actS:5*gacK*tggL:1*ataV+E*tccN*ttcL*ggtS:1*aacD*aagP*gacK*tctT*attK*tccA:1*ttcH:5*cttI*tcaA:6*attV:18*gagQ:1*gtcS*gttF*cacG:2*accK*accD:1*ttcI*aaaS*aacR:2*cctL*tctG:6*gttT:1*agtA:1*gttI:4*aacH:2*atcL:2*ttgI*gttA*gttL:1*cagE:1*aagR*cacR:1*cgtK*gttI:1*atgL +MPUK01000005.1 miniprot mRNA 611407 612204 835 - . ID=PF000013;Rank=1;Identity=0.6142;Positive=0.7715;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 21 287 +MPUK01000005.1 miniprot CDS 611407 612204 835 - 0 Parent=PF000013;Rank=1;Identity=0.6142;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 21 287 +MPUK01000005.1 miniprot stop_codon 611407 611409 0 - 0 Parent=PF000013;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 440 0 440 + MPUK01000005.1 1064489 629564 630908 588 1362 0 AS:i:846 ms:i:846 np:i:296 fs:i:0 st:i:0 da:i:90 do:i:9 cg:Z:15M4D11M7D70M1I34M1D30M1I40M1I37M3I140M2D57M cs:Z:*gagQ*attM*gtgL:2*accA:3*gccT*cttM:4-agagtccaggga:1*aacQ*gatH*gagQ:2*aacE*gaaK*caaE*cttA*gatE-gcagtcctgattgatcacctc:1*aagR*ctcV*accL*tcaR:1*gttI:1*gccT*atcL:1*gctK*tccV*ggtS*atcT*aaaD:1*attL*cagR*gacE*tccF*ttgV*tacF*gagN:1*gacE:2*atgV*cagY*gctS*ggtN*aatT*caaD*aacI*tcaP*tcaT*ccgA:3*cttG*tccT*atcM*atcL:2*gccV*caaS*tcgH*tccL*gctS:1*gacQ:1*gccS*aacT*tctT*ttcM*aatT:1*ttcY:3*ggtN*ccaM*aatR:2+S*cacE:1*gagG*atcL:1*agaK*tccA:1*aacT:3*tctD:2*gccG*gatN*gttL*ctgF*ctcS:1*gccV:1*ggtA*attV*gtgL:1*cttD*gtgR:1*actD:1*agtV*gaaQ-tct*aatE*cagI:1*attV*gccE*gccG*tccA*agaK*tctD*accL:1*cagH*gaaM*tctV:1*gacL:1*cttF*atgE:1*ctcM:3*atcH:1*gaaL*ggaK*actL:1+I*gaaH:1*aaaR:1*gaaD*gttR:2*tccT:2*ttgI:4*aagV*cttI:1*gaaS:1*attM*gttQ*attV*gctV:1*aacV*cttF*gcaR*ccaD*agaP*tctG*gttR*ctcK*aagS*aacP*attV*aatS*ggtS:1+E:2*gatY*gctF*gttL:1*aacK:2*aacA*tctN*gccT*gctQ*tctA:1*atcV*acaE*tctK:2*gctD*catL:1*aagE*gatS*ctgM*cttS*aacT*tccR*tctH*ttcK*gatL*tcaE*tccV*atcQ*gacE:1+GSL*gatE*atcV:1*aagE:2*ttaI:1*gctC:1*attS*tccA*gttI*accI:1*tcaA*atcV*gctK:2*attL*cagE:1*gccC:2*acaA:1*aatE:3*acgS*aaaR*aatG*cagK:2*ggtL*accS:2*cagL:4*cacN:10*gctS:1*gctQ:2*gccY*tccA:1*aagG:3*ggtR*actM:5*agtA*tctG*aagT:1*accS*catS:11*gctS*tccA:5*gctS*gccS:4*gctS*acaD*ttcL:2*aagQ*acgS*cagH*gatL*acgN:2*aatE:2*aagR*actK:3*gccS:1*agaK*tctM:2*aacT*caaK:1*cagR*gatS*attL:1-aaccag*agaL:1*aatD*ggtE*ggtS*gatN*cagK*gatT*attV:1*ttcY:1*aagQ:2*aacA:3*aagR:1*atgA:1*atgL:10*aatS:2*gctS*tccQ:10*tatF*ggaS:3*ggtE*gacE:1 +MPUK01000005.1 miniprot mRNA 629565 630908 846 + . ID=PF000014;Rank=1;Identity=0.4317;Positive=0.6520;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +MPUK01000005.1 miniprot CDS 629565 630908 846 + 0 Parent=PF000014;Rank=1;Identity=0.4317;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 671 0 671 + MPUK01000005.1 1064489 627800 630917 840 2229 0 AS:i:1043 ms:i:1074 np:i:392 fs:i:0 st:i:0 da:i:6 do:i:0 cg:Z:94M2I11M12I73M6I2M2I90M6I11M18I10M4I2M11I2M4I18M6I30M2D30M2I39M22D45M7D12M1D15M312U6D25M21D14M7D27M798N9M6D38M cs:Z:*aagM*acaS*atcR*tccE*gacE*tctQ*gacT:1*attE*tcaK:1*ttgI:3*actL:1*ctcG*gacG*gaaD*actV:20*agaK*cagT:3*cagL:3*ctcQ:1*gttY*cagA*ggtT:2*gttL:4*acaA:1*atcV*accL*attL:8*ggcP:3*aagV:1*gccG*atgI:2*gctI:4*ggcS:1*ggaS:1+IF:2*catD*ggtS:1*ggaD*ttcG*aagS*ggcK:2+SSRMRYIGDEYA*agaK*ctcA:1*aagG:3*gtcF*tatF:1*ttaV*agaE:2*aacQ:3*aacS:1*cgtK:4*acaI:10*accS:1*caaS*aacD:1*gacN:4*gccT*attV:4*actN:1*caaE:1*gctV*ctgA:1*gatN:2*agaK:4*agtG:2*catN*gacR*agaK+SGFGSF*aagS:1+GL*gagD:1*aagR*atcG*tctA*gctV:1*gtgI:3*gctS:1*tcaT:3*tacF:2*attV:3*ctcM*aggK:1*attL*cacY*cgcK*tccQ*acgM:1*tctD*gatY*gagD:2*gaaS*cctG:1*agaF*gaaD:2*gagT*tctE:1*catC:3*tacN:3*gctF:2*tccN*tcaT*atcV*cgcQ*tacP:1*actK*tccN:5*agaT:2*gaaS*acaD*gcaP*ccaM*aacS*ctcR:1*tccI*aaaD:2*agtD:1*gtgA:1+VNDVVF*agtE*gaaK:1*ccgS*gccK:1*agaS*caaV*actS:1*gtcL+PSRTPTRENLRDPSPVHE:1*acaP:2*ccaA*gaaR:1*atcA*tcaE*gaaV+RSLS:2+AAPVVPIATGV*actV:1+PVVT:1*aacY*ttcS:1*cagP:1*attL*gcaQ*tctV*cagV:3*gttA*gatE:2*aacS+IPSTSA*accP*caaT*caaR*aacS:1*tttV*gaaA*gccS*gaaH*cagD*cagD*agaL:1*gcaI*agaE*gaaQ*gccQ*caaK*gaaQ*aacQ*atgQ*cttI:4*cagY:1*gagL:1-cagcgt*attL*tggE*tttH*gaaQ*cagK:1*cagL*gctQ*gctL:1*gctQ:3*aggA:1*gccL:1*gaaQ*cagR*cttI*ttgQ*cgcQ*gatQ*cagE*ttgM*cagE*aacK*cagE:1+LI*gagQ*ggaQ*agaK*gtcT:1*gagS:1*gaaQ*cgtN:1*ttgI*ctcM*gcaS*atgF*aggK*caaN:3*accR:2*ttgV*ttaM:1*cagE:3*caaK:2*aagQ*accK:2*cagS:1*attL-gccaccacaacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcag:1*cagS*ttgV*actK*ttaE*tggF:1*aatE:2*gatQ:15*ctgI:1*aacK:1*tttM:2*ttaT:1*caaD*aagD*gctL:1*tcgE:1*cagE*gaaS*gccE*atcV-gagcgtcgtgagaagcttgag:1*gacK:3*aagS*aacA*gttL*gagS*ctgN*gcaV:1-ctg:2*gaaN*cgtN*gacN*agaK*gctG*agaE*ttgS*gacE*ttaR:1*aagE*attA:1*gD~gt309ag-tc-ctgattgatcacctcact*aagR*ctcS*accG*tcaM*ttgI*gttL*gatK*gccS*atcL*ttgV*gctE:1*ggtS:2*aggS*attL:2*tccP*ttgI*tacA*gagS:2-tctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaag*gccV*caaS*tcgD*tccL:3*tttL*gccS:1*tctA:1*aatI:1-ttcattgccgatggtccaaat:2*cacI*tctN*gagT:1*atcV*agaN*tccI*attL*aacP:2*acaV:1*tctL*atcL*gccL*gatR*gttC*ctgP*ctcQ*aatE*gccM*aagQ*ggtT:1~gt798ag*gatA*attE:2*tcgL*aagR*ttgC*acgL*aacS-catgagaacaagactatg*gagS*atgA*gagK:1*caaT*gtgD*gagS*attV*ttgI*aagN*ttgA*gagN*aatI:1*ttgF*gctQ*tccE*gctK*agaL:7*aagQ:1*ggaS:2*gatY:5*gagV +MPUK01000005.1 miniprot mRNA 627801 630920 1074 + . ID=PF000015;Rank=1;Identity=0.3769;Positive=0.5276;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 671 +MPUK01000005.1 miniprot CDS 627801 629349 986 + 0 Parent=PF000015;Rank=1;Identity=0.4085;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 557 +MPUK01000005.1 miniprot CDS 629659 629960 21 + 2 Parent=PF000015;Rank=1;Identity=0.2000;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 558 624 +MPUK01000005.1 miniprot CDS 630759 630920 67 + 0 Parent=PF000015;Rank=1;Identity=0.3585;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 625 671 +MPUK01000005.1 miniprot stop_codon 630918 630920 0 + 0 Parent=PF000015;Rank=1 +##PAF 1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1064 0 1064 + MPUK01000005.1 1064489 627785 630917 1800 3210 0 AS:i:2841 ms:i:2841 np:i:778 fs:i:0 st:i:0 da:i:-1 do:i:0 cg:Z:6M2D95M1D172M4I12M5I23M2D265M9I24M3I157M1I38M1I40M3I201M1D5M cs:Z:*cttM*gaaA*aagT*atgL:2-acaatc*tccA*gacE*tctA*gacE:1*attT*tcaV*tcgH*ttgI:4*tctN*ctcP*gacE:10*gcaQ:2*gtcI:4*cacN*aagH:1*tccG:1*cagA:1*ttcW:1*cagG*ctgM:1*ctgI*ctcQ:1*gttL:1*ggtN:4*ctcV:2*acaA:12*ggcP:1*gctV:1*aagR:2*atgQ*cgtQ:1*gctR*gatS:1*atcL:1*ggcS:1*ggaA:1*tcaG*gtcA:1*ggtA-gat:1*ttcR*aagH*ggcT:1*ggaA*agaK:2*aagY:3*gtcG*tatL:2*agaK:8*cgtP*ggaD:10*gtcI:2*gttR*accN*gtgI*caaD*aacD:1*gacN:3*gagQ:2*ctcV:3*actD:4*ctgI:1*gatN*cttF:4*tttI:2*atcL*tccK:1*gacG:1*aagP*tctN:2*aagR:4*gtgI:10*aagR:1*attT:1*tctT:4*attM:1*cgcA*tccT:1*ggaN*tctD:1*gagD:6*gaaS:2*gagY:3*gccQ:2*tacL:3*gctY:4*atcL*cgcK:4*ttgV:4*agaT:1*ccaS*gaaQ*acaD*gcaP:3*atcY*tccG*aaaE*gatN*gagP*agtE*gacE*gtgG:1*agtA+PALP*gaaK:2*gccI:1*agaE*caaV*actA:1*gtcL:2+GATTA:1*gttS*ccaT*gaaT*ccaD*atcL*tcaT*gaaP*cagE:1*actV*atgV*ggtD*aacF*ttcW*caaS*cagT:1*attQ*gcaG*tctQ:1*ccaQ-actggt*gttL*gatQ*ttcQ*tggQ*aacQ*accL:2*aacQ*gtgQ*tttY:1*gccE:2*cagR:3*agaA*gaaQ*gccR*caaE*gaaA*aacE*atgL*cttQ:3*caaM*cagL*gctH*gagL:2*cgtQ*attQ*tggQ:2*cagE:2*gctR*gctM:10*cagA:5*ttgY:1*aacR:2*gagD:2*gtcA:6*ttgI:1*gcaN*atgL:1*caaG:3*accR:3*ttaM:1*cagE:3*caaR:3*accG:2*cagG:1*attL*gccQ*accQ*acaV*acaI*cagA*actN*gctT*actN*cagL:1*cttS*ggtQ:3*gagD*cttM*ttgI*aacR:5*ttgV*actN*ttaS*tggL:1*aatS:3*tctV:13*aacD*ctgK:1*aacK*aagE*tttL:1*aagL:1*cagK*caaV:10*cgtK:5*aagR:1*ttgI:1*tctA:2*gttI:3*ggtD:9*ttgY:2*gagD:1*attS*aagR:1*ggtN*aaaQ*gacK*agcD*gagV:1*gatE:3*ctcR:1*atcL:1*gacL*ttgT*acaE*ttgD:2*gatA:1*tctA:3*cagK*tccG*tcgA*aacD:2*tcgL*gtgL*tttI*gctS*tctK:1*aagN:2*atgL*gaaA:2*aagE*gcaE*aaaA:1*gagK*acaA*cagK*tctQ*cgtS*gctV:1+DNYANRSFD*aacD*ccaS:2*gaaK*cagL:2*aaaA:2*gaaD*gagD:4*gtgL:1*caaE:1*atgV:1*gatS+MEA:2*cagK:1*cttM*gccS*agaL*gtcN*cagH*ggaN:1*aacA:1*gagQ:1*attL*aacE:2*cttI:1*gcaE:2*attK*gatE:2*actS:2*accN:1*ttgI*gttI:1*gccS*atcV:1*gctE:1*ggtS*atcA*aaaE:1*attV:1*gacE:2*tacF:1*ctgF*gacE:10*tcaT:7*atcV:3*caaS*tcgT:6*aacT:3*gacN:2*gccI:6*cacY:1*gagG:2*agaS*tccT*attA*aacT*gaaI*ttcL*acaS:1*tctV:1*gccP:3*ctcN:4*attL*gtgT:2*gtgA*aagR*actD:1*agtT*gaaV*tctA*aatD*cagN*ctcI:1*gccS*gccY*tccA:1*tctL*accA:1*cagV*gaaA*tctV:3*cttF*atgS*tctG*ctcV*ctcQ:1*gatN+K*atcL*cttA*gaaQ*ggaL*actS*gagD:1*gagD:2*gaaD*gttK:2*tccN*ggtF:1*ttgI*aacD:2*gagT*aagH:2*gaaS:1*attS*gttQ*attV*gctS:1*aacK:1*gcaT:2*tctA+G*gttT*ctcH*aagL:1*attG*aatS:5*gctL:2*aacR:1*ttgM*aacA*tctR:2*tctE:2*acaE:2*gctN:1*catK:1*aagN*gatE:2*aacS*tccR*tctP*ttcK:1+PKF:1*tccV*atcF:1*ggtL*gatK:1*aacH*aagE:3*tctA:3*tccA:2*aacT*tcaA:2*cttA:2*cagK:2*attS*gagD*acaC:1*aatQ:3*acgA*aaaQ*aatG*cagK:1*agtA*ggtS*accS:1*gccT*cagA:17*gctS:5*aagN*atcV:2*ggtE:7*tctG*aagN*aacH:1*catP:1*gaaQ:9*gctS:13*acaS*ttcY:6*acgR:2*aatG:3*actA:16*aacS*cagK*agaK*acgQ*aatQ*ggtK*ggtE*gatE*cagN:1*attV:1*ttcY:4*aacP:1*gagV*aacL:2*atgT:13*gccS:1*gctN:12*ggaS:1*agaV:1-ggt:1*agtE:3 +MPUK01000005.1 miniprot mRNA 627786 630920 2841 + . ID=PF000016;Rank=1;Identity=0.5607;Positive=0.7271;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +MPUK01000005.1 miniprot CDS 627786 630920 2841 + 0 Parent=PF000016;Rank=1;Identity=0.5607;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +MPUK01000005.1 miniprot stop_codon 630918 630920 0 + 0 Parent=PF000016;Rank=1 +##PAF 4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1053 0 1046 + MPUK01000005.1 1064489 627794 630914 1641 3192 0 AS:i:2622 ms:i:2622 np:i:763 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:99M4I179M1I13M3I14M6D6M4I14M2I16M2D226M5I22M1D23M8D133M1I34M1D32M4I211M cs:Z::2*aagS*acaN*atcR*tccA*gacE*tctV:2*attN*tcaV*tcgN*ttgI:4*tctN*ctcT:2*actS:14*tggH:2*aagR:2*agaK*cagA:1*ttcW:1*cagG*ctgI*aaaR*ctgI*ctcQ:1*gttL:1*ggtT:1*gaaD:1*caaM*ctcV:2*acaA:12*ggcP:3*aagR*gagD:1*atgQ*cgtA:1*gctV*gatN*tggF:1*gagQ*ggcS:1*ggaA:1*tcaG*gtcN*catN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aagS:3*gtcR*tatF:1*ttaM*agaQ:2*aacT:3*aacY:1*cgtP*ggaA:3*acaM:4*gagD:1*gtcI:2*gttR:2*caaN*aacD:1*gacN:6*ctcM*gatN:6*gctG*ctgV:2*cttF:3*atcV:1*gctS:1*atcL*tccQ*catR*gacS:1*aagS*tctN:5*gctS:3*ttgM*gttI:7*aagR:1*attV*acgI:5*attM:1*cgcV:3*tctN:5*cctS:2*gaaQ:1*tatF*gagY:3*gccD:2*tacR*gagD:2*gctY:3*tcaT*atcL*cgcK:2*actR:1*ttgI*attV:3*agaQ:1*ccaG*gaaK*acaN*gcaP:3*atcL*tccE*aaaE*gatE*gagD*agtG*gacP*gtgG*gaaL*agtP*gaaQ:2*gccK:1+V*agaA*caaE:1*tcgR*gtcS*gctV*acaS:1*gttE:1*gaaA:1*atcL+PVA*tcaT*gaaP*cagT:1*actS*atgI*ggtP*aacA*ttcE*caaA:3*gcaE-tctcagccaactggtgtt*gatN:2*aacS*accQ*caaD+ALLA:1*aacQ*gtgQ*tttY*gaaD:3*cagE:2*gcaR*agaQ*gaaQ+QA*gccM*caaE:1*aacE*atgR*cttI*caaR:1*cagM:1*cagM*gctQ*gagQ:2*cgtQ-atttgg:2*cagM:2*gctR*gctQ:1*gctM:1*cagA:1*aggQ*ctaR:5*ttgM*cgcA:2*ttgM*cagA*aacR*cagH:1*gagQ:2*gtcM:6*ttgI:2*atgL:1*caaG:2*gagD*accQ:3*ttaM:1*cagE:3*caaG:3*accA:2*cagA:1*attL*gccN*accQ*acaL*acaQ:3*actH:1*caaS*cttA*ggtQ*tctA:2*gagD:1*ttgI*aacE:3*gaaQ:1*ttgI:1*ttaM:1*aaaR*aatQ:2*gatE*tctT:3*ctgR:2*cagS*ttgM:1*cagE:1*catY:1*aacA:2*aacK:1*tttL:1*aagA*ttaT:7*cagK:4*cgtK*cgtA:4*aagR:1*ttgM*aagR*tctH:2*gttI:3*ggtD:2*agaK:6*ttgY:2*gagD*aagR*attA:3*aaaN*gacK*agcE*gagD*attV*gatE*aagR:2*ctcR*cagE*atcL:1*gacM*ttgA*acaQ*ttgD:2*gatA:1*tctK*gagD:2*cagT*tccG*tcgA*aacD:2*tcgL*gtgL*tttL*gctS*tctK:1*aagN:2*atgL*gaaS*gacE:1*aagE*gcaN*aaaA:1*gagK*acaM*cagK*tctQ:3+DERGD*aacD*ccaS:2*gaaL*cagR*aagR:1*aaaE:3*gagI:1*cttY:1*attA*gtgL*cagN*caaE*accA*atgF-gat*gatN*gccS:1*cagA*gagL*cttH*gccQ*agaE*gtcQ:2*gagN:1*gatS*gagN*gctG*attG*aacS*gaaT*caaP:1*gatA:1-gtcctgattgatcacctcactaag:1*accH:1*ttgI*gttI:2*atcL:1*gctE:2*atcS*aaaQ:1*attV:2*tccA:1*tacF:2*gacE:10*tcaT:6*atcV:4*caaS*tcgE:2*accS*gacA:2*aacT:3*gacN:1*attL:2*ggtE*ccaT*aatD:2*cacY*tctA:3*agaK*tccT*attV:1*gaaI*ttcY*acaS*tctT*tctA*atcV*gccE*gatN:2*ctcS:1*gccS:2*attL*gtgT:2*gtgA:1*actD:1*agtA*gaaS*tctA*aatD*cagA:1*attV*gccN*gccF*tccA:1*tctE*accS:1*cagE*gaaA*tctT:1*gacR*ttcN*cttF*atgI*tctG:3*gatE:1*cttI:1*ggaD*actF*gagP+L*gaaD*gagD:1*accM:1*gttR:2*tccT*ggtL:1*ttgM:3*gagT*aagA:2*gaaH:1*attT*gttK*attL:2*aacR*cttM:2*agaK*tctT*gttN*ctcI-aag:1*attM*aatS:5*gctL:1*gatE*aacR:1*ttgM*aacA*tctK:2*tctD:2*acaA*tctA:1*gctS:1*catK:1*aagG:3+KFNQ*aacS*tccD*tctP*ttcL*gatK:1*tccT*atcT:1*ggtL*gatQ*atcL*aacH*aagE:1*atcA*ttaI*tctQ:2*attQ*tccA:1*accI:1*tcaA:2*cttA:2*cagR:2*attT*gagD*acaA:5*acgA*aaaQ*aatG*cagR:1*agtT*ggtS*accS:7*cacN:1*agaK:10*gctS:5*aagN:3*ggtE*actK:3*gtgT:1*agtR*tctR*aagT*aacS*accG*catL:8*gaaN:24*acgK:2*aatE*gctC:2*actV:6*tctN:2*aacK:3*gatE:3*cagK*agaK*acgF*aatG*ggtE*ggtL:1*cagE*gatK*attV:1*ttcY*tcgA*aagA:1*acgS*aacK:2*aacF:2*atgT:13*gccD:1*gctQ*tccG:6*gagQ*attM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gatE:1 +MPUK01000005.1 miniprot mRNA 627795 630914 2622 + . ID=PF000017;Rank=1;Identity=0.5141;Positive=0.7171;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +MPUK01000005.1 miniprot CDS 627795 630914 2622 + 0 Parent=PF000017;Rank=1;Identity=0.5141;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +##PAF 4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1054 0 1047 + MPUK01000005.1 1064489 627794 630914 1641 3195 0 AS:i:2611 ms:i:2611 np:i:763 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:99M4I179M1I13M3I14M6D6M4I14M2I16M2D226M5I22M1D23M8D133M1I34M1D25M1I7M4I211M cs:Z::2*aagS*acaN*atcR*tccA*gacE*tctV:2*attN*tcaV*tcgN*ttgI:4*tctN*ctcT:2*actS:14*tggH:2*aagR:2*agaK*cagA:1*ttcW:1*cagG*ctgI*aaaR*ctgI*ctcQ:1*gttL:1*ggtT:1*gaaD:1*caaM*ctcV:2*acaA:12*ggcP:3*aagR*gagD:1*atgQ*cgtA:1*gctV*gatN*tggF:1*gagQ*ggcS:1*ggaA:1*tcaG*gtcN*catN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aagS:3*gtcR*tatF:1*ttaM*agaQ:2*aacT:3*aacY:1*cgtP*ggaA:3*acaM:4*gagD:1*gtcI:2*gttR:2*caaN*aacD:1*gacN:6*ctcM*gatN:6*gctG*ctgV:2*cttF:3*atcV:1*gctS:1*atcL*tccQ*catR*gacS:1*aagS*tctN:5*gctS:3*ttgM*gttI:7*aagR:1*attV*acgI:5*attM:1*cgcV:3*tctN:5*cctS:2*gaaQ:1*tatF*gagY:3*gccD:2*tacR*gagD:2*gctY:3*tcaT*atcL*cgcK:2*actR:1*ttgI*attV:3*agaQ:1*ccaG*gaaK*acaN*gcaP:3*atcL*tccE*aaaE*gatE*gagD*agtG*gacP*gtgG*gaaL*agtP*gaaQ:2*gccK:1+V*agaA*caaE:1*tcgR*gtcS*gctV*acaS:1*gttE:1*gaaA:1*atcL+PVA*tcaT*gaaP*cagT:1*actS*atgI*ggtP*aacA*ttcE*caaA:3*gcaE-tctcagccaactggtgtt*gatN:2*aacS*accQ*caaD+ALLA:1*aacQ*gtgQ*tttY*gaaD:3*cagE:2*gcaR*agaQ*gaaQ+QA*gccM*caaE:1*aacE*atgR*cttI*caaR:1*cagM:1*cagM*gctQ*gagQ:2*cgtQ-atttgg:2*cagM:2*gctR*gctQ:1*gctM:1*cagA:1*aggQ*ctaR:5*ttgM*cgcA:2*ttgM*cagA*aacR*cagH:1*gagQ:2*gtcM:6*ttgI:2*atgL:1*caaG:2*gagD*accQ:3*ttaM:1*cagE:3*caaG:3*accA:2*cagA:1*attL*gccN*accQ*acaL*acaQ:3*actH:1*caaS*cttA*ggtQ*tctA:2*gagD:1*ttgI*aacE:3*gaaQ:1*ttgI:1*ttaM:1*aaaR*aatQ:2*gatE*tctT:3*ctgR:2*cagS*ttgM:1*cagE:1*catY:1*aacA:2*aacK:1*tttL:1*aagA*ttaT:7*cagK:4*cgtK*cgtA:2*cttM:1*aagR:1*ttgM*aagR*tctH:2*gttI:3*ggtD:2*agaK:6*ttgY:2*gagD*aagR*attA:3*aaaN*gacK*agcE*gagD*attV*gatE*aagR:2*ctcR*cagE*atcL:1*gacM*ttgA*acaQ*ttgD:2*gatA:1*tctK*gagD:2*cagT*tccG*tcgA*aacD:2*tcgL*gtgL*tttL*gctS*tctK:1*aagN:2*atgL*gaaS*gacE:1*aagE*gcaN*aaaA:1*gagK*acaM*cagK*tctQ:3+DERGD*aacD*ccaS:2*gaaL*cagR*aagR:1*aaaE:3*gagI:1*cttY:1*attA*gtgL*cagN*caaE*accA*atgF-gat*gatN*gccS:1*cagA*gagL*cttH*gccQ*agaE*gtcQ:2*gagN:1*gatS*gagN*gctG*attG*aacS*gaaT*caaP:1*gatA:1-gtcctgattgatcacctcactaag:1*accH:1*ttgI*gttI:2*atcL:1*gctE:2*atcS*aaaQ:1*attV:2*tccA:1*tacF:2*gacE:10*tcaT:6*atcV:4*caaS*tcgE:2*accS*gacA:2*aacT:3*gacN:1*attL:2*ggtE*ccaT*aatD:2*cacY*tctA:3*agaK*tccT*attV:1*gaaI*ttcY*acaS*tctT*tctA*atcV*gccE*gatN:2*ctcS:1*gccS:2*attL*gtgT:2*gtgA:1*actD:1*agtA*gaaS*tctA*aatD*cagA:1*attV*gccN*gccF*tccA:1*tctE*accS:1*cagE*gaaA*tctT:1*gacR*ttcN*cttF*atgI*tctG:3*gatE:1*cttI:1*ggaD*actF*gagP+L*gaaD*gagD:1*accM:1*gttR:2*tccT*ggtL:1*ttgM:3*gagT*aagA:2*gaaH:1*attT*gttK*attL:2*aacR*cttM:2*agaK*tctT*gttN*ctcI-aag:1*attM*aatS:5*gctL:1*gatE*aacR:1*ttgM*aacA*tctK:2*tctD:2*acaA*tctA:2+S:1*catK:1*aagG:3+KFNQ*aacS*tccD*tctP*ttcL*gatK:1*tccT*atcT:1*ggtL*gatQ*atcL*aacH*aagE:1*atcA*ttaI*tctQ:2*attQ*tccA:1*accI:1*tcaA:2*cttA:2*cagR:2*attT*gagD*acaA:5*acgA*aaaQ*aatG*cagR:1*agtT*ggtS*accS:7*cacN:1*agaK:10*gctS:5*aagN:3*ggtE*actK:3*gtgT:1*agtR*tctR*aagT*aacS*accG*catL:8*gaaN:24*acgK:2*aatE*gctC:2*actV:6*tctN:2*aacK:3*gatE:3*cagK*agaK*acgF*aatG*ggtE*ggtL:1*cagE*gatK*attV:1*ttcY*tcgA*aagA:1*acgS*aacK:2*aacF:2*atgT:13*gccD:1*gctQ*tccG:6*gagQ*attM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gatE:1 +MPUK01000005.1 miniprot mRNA 627795 630914 2611 + . ID=PF000018;Rank=1;Identity=0.5136;Positive=0.7164;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +MPUK01000005.1 miniprot CDS 627795 630914 2611 + 0 Parent=PF000018;Rank=1;Identity=0.5136;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +##PAF ONH67106.1 254 0 254 + MPUK01000005.1 1064489 616189 618214 762 762 0 AS:i:1262 ms:i:1299 np:i:254 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:69M1263N185M cs:Z::69~gt1263ag:185 +MPUK01000005.1 miniprot mRNA 616190 618217 1299 + . ID=PF000019;Rank=1;Identity=1.0000;Positive=1.0000;Target=ONH67106.1 1 254 +MPUK01000005.1 miniprot CDS 616190 616396 348 + 0 Parent=PF000019;Rank=1;Identity=1.0000;Target=ONH67106.1 1 69 +MPUK01000005.1 miniprot CDS 617660 618217 951 + 0 Parent=PF000019;Rank=1;Identity=1.0000;Target=ONH67106.1 70 254 +MPUK01000005.1 miniprot stop_codon 618215 618217 0 + 0 Parent=PF000019;Rank=1 diff --git a/results/2026-10-30_petasospora/data/GCA_022641835.1_ASM2264183v1.miniprot.gff b/results/2026-10-30_petasospora/data/GCA_022641835.1_ASM2264183v1.miniprot.gff new file mode 100644 index 00000000..f7114171 --- /dev/null +++ b/results/2026-10-30_petasospora/data/GCA_022641835.1_ASM2264183v1.miniprot.gff @@ -0,0 +1,87 @@ +##gff-version 3 +##PAF MH260578_SUI1 109 0 109 - CP048715.1 3128646 610677 611004 267 327 0 AS:i:488 ms:i:488 np:i:103 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:109M cs:Z::2*tctN:1*cagE:2*aacK*actS:10*gatE:1*cagE*ccaV:30*agaK:2*cgtK:1*ttgI:2*gacE:24*gtcL:1*gttI:3*ttgM*accV*tccQ*cagT:1*gccG*ttgI*cagK:9 +CP048715.1 miniprot mRNA 610675 611004 488 - . ID=PF000001;Rank=1;Identity=0.8165;Positive=0.9450;Target=MH260578_SUI1 1 109 +CP048715.1 miniprot CDS 610675 611004 488 - 0 Parent=PF000001;Rank=1;Identity=0.8165;Target=MH260578_SUI1 1 109 +CP048715.1 miniprot stop_codon 610675 610677 0 - 0 Parent=PF000001;Rank=1 +##PAF MH260578_SLA2 1072 29 1072 + CP048715.1 3128646 641766 644868 1707 3156 0 AS:i:2757 ms:i:2757 np:i:774 fs:i:0 st:i:0 da:i:21 do:i:0 cg:Z:94M2D73M1D86M1I24M3I24M2I256M5I54M4D129M1I41M3I39M3I150M2D55M cs:Z:*tccT:1*cttI*attS*tcaT*tcgA*ttgI:3*actC*tctT*ctcP*gacE*gaaD:2*ccaS:18*agaK*cagD:1*ttcW*aacE*cagT*ctgM:1*ctgV*ctcV:1*gttL*cagK*ggtN*gatT*gaaD*gttT:4*acaA:2*accL:9*ggcP:3*aagV*gagG*gccG*atgF*cgtK:1*gctM*gatE:2*gagS*ggcN:1*ggaD*agaT*tcaL*gtcR*catT*ggtL:1-ggattc*aagR*ggcR*tatF*ggaQ*agaV:2*aagP:3*gtcN:1*ttgI:1*agaQ:2*aacR:2*aagH:17*gttR:2*caaS*aacD:1*gacN:3*gagD:5*atgT*actS:3*gctS:2*gatS*cttF*caaG:1*ctgD:3*agtT*atcV-tcc*catR*gacN*agaN*aagN:3*aagI:6*ttgI:10*attL*acgI:5*attM*cacY*cgcK*tccM*acgS*ggaD:1*gatA:1*gcaI:4*agaK*gaaS*cgcK*tatF:1*tctD:2*gccE:3*gagD:3*gacN:2*tcaN:5*tccT:5*agaK:2*gaaF*acaE:4+I:1*tctQ*aaaN:2*agtD*gacE*gtgN*gaaV*agtN*gaaL*agaP:1*gccL*tcaT*agaE:1*actK*tcgT*gtcA:2*ccaT*gttL+RHS:1*gaaT:1*atcF*tcgE*gaaQ*cagE*ccaN*actE*atgR*ggtQ*aacQ*ttcQ:1*cagA:3*tctT:4*gttM+VR*gatN*ttcA*tggF*aacV*accE:2*aacQ*gtgL*tttY:1*gccQ*gaaQ:2*agaQ:1*gcaE*agaQ*gaaQ*gccR:1*gaaA*aacE*atgL*cttQ:5*gctQ*gagL*caaA:1*cgtQ*attQ*tggY*tttW:4*gctR*gctQ:3*cagA:1*aggQ:3*gaaQ:3*cgcL:2*ttgA:1*aacR*cagH:1*gagQ:9*ttgI:1*gcaT*atgL*aggK*caaG:2*gagD:4*ttaM:2*cagS:3*agaK:1*aaaQ*accA:2*cagN:1*attL:1*accS:5*actQ*cagE:2*ggtA:4*cttQ:2*agtL:4*ttgV*actE*ttaY:5*gatE:10*cagT:6*aacT:1*tttS:2*ttaI:12*cgtK:5*aagR:1*ttgM:1*tctA:2*gttI:3*ggtD:2*agaK:7*gacE:2*aaaR*attF:1*ggtN:1*aaaT*gacK*agcT*gagD*attL*gatQ*aagT:1*gagQ:1*cagE*atcK*aggD*gacE:1*acaE*ttgQ:2*gatA*gacT*tctL*gagD:1*agcA:2*tcgA:2*tcgT*tcgA*gtgI:1*gctN*tctQ:1*aagN*agaK:1*atgL*gaaQ*gacE:1*aagQ*gcaN:1+FKDVT:1*gagN*acaA*cagP:1*cgtD*gctE*ctgK*aacL*ccaK*gacQ:2*cagE*aagQ:1*aaaQ:2*gaaD*gagL:1*cttI:1*attM*gtgM:5*gatE*gccT*cttI*cagK*gagD:1*gccV*agaK*gtcT:1*ggaN*gagS*aacR*gatE:1*gctK*attS*aacE*gaaM:1*cttN*gatK*gcaL-gtcctgattgat*cacN*ctcS:2*ctcV*acaL*tcaG:1*gttI:4*gctK:3*aaaN:4*tccA*ttgV*tacF:10*caaL:6*ttgV:1*tccT*atcL*atcL:2*gccS*caaS*tcgG*tccL*gctV:4*aacS:3*gacN*ttcM*attL*gccV:2*ccaD:1*ggtA*gatN*cacE*tctA*gagL*atcV:1*agaD:2*aacT*gaaN:2*tctT:2*gccS:1*gttI:3*gccT:2*attL*gtgT:2*gtgT:1*actQ:1*agtD*gaaF*tctQ*aatD*cagD:2*gccD*gccT*tccA*agaK*tctD*accI:1*cagE*gaaI:2*gacV*ttcY:1*atgE:2*ctcY+H:1*gatN*atcL*cttV*gaaN*ggaK*actS*gagI*gaaD*gagD*aaaQ:1*gaaD*gttI:2*tccN:2*ttgI*aacD:3*aagI:2*gaaT:1*attL*gttQ*attL*gctV:1*aacS*cttM*gcaK*ccaT*agaP:1*gttS+KID:1*aagS*aacK*atcV*aatD:1*gatE:1*ggtS:1*gcaL:2*aacK:1*ttgM*aacE*tctH:1*gctS*tctR*gctV:1*acaE*tctE:1*gctS*gctV:2*aagS*gatS:3*tccQ*tctP:2+VSM:4*ggtI*gatE*atcV:2*gccS:2*tctA:2*attS:1*gttI*accI*aacT*tcaA:1*gctK*cttY:2*cagR:1*gccS:2*acaS:1*aatE:3*accN*aaaN*aatG*cagR:2*ggtH*accS*cgtK*gccT*cagS:4*cacN:1*agaK:8*gctS:1*gctS*gttI:1*gctY:2*aagN*atcT:2*ggtR*actI:5*agtQ*tctN:1*aacH*accS*catN:3*attV:3*aatR:10*gctS:1*tcaA:3*gctS*acaQ:6*gatN*acgK:2*aatD:3*actK:1*acgN*tctL:2*agaK*tctQ:2*aacD:2*cagN*gatK*attL*ttgI*aacI*cagG*agaK*acgH*aatE-ggtggt*gatV*cagE:3*ttcY:3*acgS*aacI:5*atgA:15*gctS:11*tatF*ggaS:1*agaK:1*ggtD:5 +CP048715.1 miniprot mRNA 641767 644871 2757 + . ID=PF000002;Rank=1;Identity=0.5409;Positive=0.7357;Target=MH260578_SLA2 30 1072 +CP048715.1 miniprot CDS 641767 644871 2757 + 0 Parent=PF000002;Rank=1;Identity=0.5409;Target=MH260578_SLA2 30 1072 +CP048715.1 miniprot stop_codon 644869 644871 0 + 0 Parent=PF000002;Rank=1 +##PAF MH260578_TGL1 440 12 440 + CP048719.1 993546 374835 376110 582 1314 0 AS:i:905 ms:i:905 np:i:268 fs:i:0 st:i:0 da:i:27 do:i:51 cg:Z:15M2I11M2D4M5D23M1D51M4I229M7I20M1D29M1D33M cs:Z:*tcgA*ctcV:1*gagQ*tggL:2*gtcL*gtgI*gccF*tcgH*ttcK:1*gctG:1+EE:2*gagS*tttY*gtaF*atcQ*tccT*cttP*atcS*accN:1-gccatc:2*tcaN*gtgI-ctcgacctcgttaca:1*ttcA*atcV*caaS:1*gtgE*cacN*tctK:1*atcK*gttI*ccaS*gaaP*aagS*atcA:1*aacP*ggtL:1*ataE*ctcV*gagA:1-gct*gccT*aagH*aagC*atcL*acaH*aagQ*gatK:1*aggI:2*cctN*tcaD:1*caaV*cacD:1*ggaV:1*ctcA*tttH:2*caaK*attV*gatH*gatE:3*cctQ:1*gcaK:5*tgtS*cttI:3*aggL*ccaK*ctcS*aagS*gaaG*ggcD*gcaK:1+QNSR:9*atgT*tgcN:1*gatE*atcL*tggF*gttL*gtcL*aacG*atcE*aagN:2*agaK*aatC:3*attL:1*catV:1*atgQ:1*tttY:3*atgL:9*acaR:3*tccF:1*aagS*ccgS*tcaT*catD*aagE*agaK:2*gacN*ttcY:1*atcL:5*tatF:3*gatN*tccT*atcV:4*tcaR*tacN:1*aagQ:1*caaD*gacS:1*acaS:10*gcaC*tttL:5*catN*gatH*aaaD:2*accK*cagK:1*aagN*ctcK:2*gctG:1*gctS:3*acaI:1*agtQ:2*cacN:1*caaP*ttcI*gtcA*gatS*acaF:1*atgV*aaaT:1*acaA:1*aacG*ttcL*atgL:1*ctgR*gccI:4*ctcA*ctcI:2*acaS*gcaV*tcaV:2*aaaQ*aatK*atcL*tgtM*tacG:1*ccaV:1*ttcY*tctE:1*atcV*atcV:1*cttK:1*gttL*cgcV*atgF:2*gacN:2*aatS*tacG:2*actK*catT*gatS:2*attK*gctV*gcaG*ttcY*tccP:1*ctcM*tacF:2*acaS:5*gttA:6*cgcD*aatS*ggtK*gcaR:2*ttgM:3*gacG+GASGSRL:1*tcaY:1*accS*aatG*gccT*ctcS*tctG*gctK*ccaT*ttcN:1*aacV:7-aat:1*aaaT*gtaT:1*atcC*aagL*ttgF*attV:2*tcaK*attS:1*tccM:1*gtgI:2*gatE*aagR*atgT:1*tcaD*ttaN:1*ccaS*gcgC:2-aca*gatQ*gctT*gtaI*gcaG:1*ccaD*aatG*catY:2*ttgM:1*atcT*atcL:1*ggtA:1*cacQ*attV:2*ctcK:1*ttaF:1*catV*ttgV*ttcL*aatQ*ttcY:1*ggtK*gtcQ +CP048719.1 miniprot mRNA 374836 376110 905 + . ID=PF000003;Rank=1;Identity=0.4429;Positive=0.6119;Target=MH260578_TGL1 13 440 +CP048719.1 miniprot CDS 374836 376110 905 + 0 Parent=PF000003;Rank=1;Identity=0.4429;Target=MH260578_TGL1 13 440 +##PAF MH260578_TGL1 440 79 440 + CP048726.1 253472 188197 189259 528 1101 0 AS:i:889 ms:i:889 np:i:238 fs:i:0 st:i:0 da:i:54 do:i:45 cg:Z:90M1I91M1I86M11I10M3D34M3D37M cs:Z::1*tttI*gcaV*tctD*cttI*gctV*gcaH:1*caaH:2*cagK:1*caaH:2*actI:3*tccK:5*actS*ctcI:2*gtcI*tttL*gaaK*aagS*gaaS*gttG*tccD*tgcK*cagP*aagQ*ggcN*aagS*cctR:1*gctV:1*ttcL:11*atcL:2*gacE*cgcN*cgtK*gatE:4*tatF:2*ttgV*gagD*aagQ:15+R*tccK:1*attL*acaF:1*actS*gagS*tctT:1*ccaE*cgcK*tacF:1*gacN*ttcY:6*cacM*acaF:3*tctN*attT*atcV:4*gacR*ttcN:1*ggcQ:1*ccaD:2*actS:7*acaS*acaA:1*atgC*tttL:2*atgL:1*attL:1*gaaH:3*tctK:1*gtcI:1*ttcK:1*gttI*gctG:3*gtgA:4*ccaG:1*actN*cacN:1*ctaI*atcA*aagS*ccaF:2+T*caaS*actA:1*tcaG*aatL*atcL*accY*aagR*gtgI:2*agcK*ggaR*cctA*ttaI:2*ttcS:1*aacV*cttF*gtcW*aatQ:1*gccL*atcM*ccgG*cttP*cctV:2*aagE*accK*atcV*attV:1*gtcK:2*ggtV*attF:3*accW*tcaK*ggaS*gccG:2*agcK*tcgT*aagS:2*ctcK*gcaV:3*tacH*ttgM:2*gttP:4*tgtS:1*gtaA:6*agaD*cagS*cagK*tccR:1*cacQ*aagM*tacF+DEGGASGSRLV:1*aaaL*gctS*gctG*gacT:1*aaaG*atgK:2-gacctctgg*gagR:1*gagA*ctcS:1*gatP*atgT*aaaQ*aatT*ctgI*aaaT*gtgT:3*ttgF:3*gacK*gctS:1*atcM:1*gtcI:2*gatE*aagR:1*aggL*cagD*catN*ttcL-aagcagtca*aacS*ctgC:1*gttI:3*aagG*gtgI*agtD:11*gagQ:1*ttgE*tatK*acgK:2*gcaP*cctV:2*gagQ*aagY*attL*gaaK*gagQ +CP048726.1 miniprot mRNA 188198 189259 889 + . ID=PF000004;Rank=2;Identity=0.4796;Positive=0.6485;Target=MH260578_TGL1 80 440 +CP048726.1 miniprot CDS 188198 189259 889 + 0 Parent=PF000004;Rank=2;Identity=0.4796;Target=MH260578_TGL1 80 440 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha_MATa1 181 123 178 + CP048715.1 3128646 631962 632127 108 165 0 AS:i:187 ms:i:187 np:i:42 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:55M cs:Z::3*acaR*tcaH:1*accP*cctE*gaaS:3*tttL:2*agaK*gtgA:2*gctV:2*actF:3*acgK:2*aaaE*gttR:2*gaaR*aagE:2*ctcI*acgS:12*atgA:1*aacS:1 +CP048715.1 miniprot mRNA 631963 632127 187 + . ID=PF000005;Rank=1;Identity=0.6545;Positive=0.7636;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 124 178 +CP048715.1 miniprot CDS 631963 632127 187 + 0 Parent=PF000005;Rank=1;Identity=0.6545;Target=460523_cbs-4732_MATog_MATa-MATalpha_MATa1 124 178 +##PAF 5476_sc5314_MTL_A_MTLA1 210 0 198 + CP048715.1 3128646 608130 632130 186 621 0 AS:i:170 ms:i:203 np:i:98 fs:i:0 st:i:0 da:i:294 do:i:3 cg:Z:28M2I46M23388U21M6D43M3D57M cs:Z:*tgtM*gaaN:1*aagE:1*ggcE*gaaS*gtcS*aaaL*tcgT*actL*attL*tcgK*aatS*cttV:2*acgL*atgV*agaQ*gagA:2*gatV:1*gttK*gtcN:1+DN:2*cttI*cgtF:1*gctQ*gacL*tctK*gctR*aatE*gagR*gagQ*cgcE:1*atcS*cgtN*gctS*aatH*cttE:1*ctcT*cgtE*gaaT*ctcF*aatF*gtgH*cgcE*ctcS*gagL:1*attR*cagL*aacM*aacK*attL*gctS:2*gatA*gctG*gagS:1*gccV*gagQ*tccY:1*cL~gt23385ag-ta*ccaS*aggL*gaaN*accN*atcL*ttcY*aagN*actL*tatL*aaaL:1*attT*ctgR*gagK*agaQ:1*gttE*gatE*cgaE:1*cttF-gttgatggaatattgagc:1*ccaF:1*aaaT*gaaV*gacS*cccT:1*gcgE*tacF*ggaA*cacL*agaE*ataQ*gcaI*tcgT*tacF:2*cagS*tctD*accQ*actM*atcD*gaaD*gtaD*acgK*ggaD:1*actE*tctD*agcD*cctI*gtcL*tttI*ccaK:1*ggaE*ggtE*cgcI:1*cgaK*aatS-gcgcttcca*gcaK:3*acaQ*tcaR*attL*accD*cctN*gaaS*tccT:5*agaK:2*aagE*gctK*aagN*aggK*actQ:2*agtR*acgR:2*aaaE*gttL:4*tgtH:1*ctcV*acgS*ccaL*ctgS:1*gttI:10*aacK:2 +CP048715.1 miniprot mRNA 608131 632130 203 + . ID=PF000006;Rank=1;Identity=0.2995;Positive=0.4734;Target=5476_sc5314_MTL_A_MTLA1 1 198 +CP048715.1 miniprot CDS 608131 608353 42 + 0 Parent=PF000006;Rank=1;Identity=0.2468;Target=5476_sc5314_MTL_A_MTLA1 1 76 +CP048715.1 miniprot CDS 631739 632130 161 + 2 Parent=PF000006;Rank=1;Identity=0.3308;Target=5476_sc5314_MTL_A_MTLA1 77 198 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1019 7 1019 + CP048715.1 3128646 641760 644859 1560 3129 0 AS:i:2424 ms:i:2424 np:i:724 fs:i:0 st:i:0 da:i:15 do:i:9 cg:Z:256M2D10M1D15M2D10M4D5M2I31M1I8M1D218M1I8M7D22M4D11M7D70M1I34M1D30M1I40M1I26M1I11M2I140M2D57M cs:Z::1*gacN*tccL:1*cttI*attQ*tcaA:5*actC*tctT*ctcA:1*gaaA*actA:21*cagE:1*ttcW*aacH*cagC:5*gttI:1*ggtS*gatN*gaaD*gttT:1*ctcI:5*accV:9*ggcP*tctT*gctC:1*aagI*gagG*gccG*atgY*cgtK:1*gctI*gatS:1*atcL:1*ggcS:1*ggaS:1*tcaF*gtcS*catN*ggtN*gatG*ggaT*ttcA*aagA:2*ggaT:7*gtcF:2*ttaE*agaQ:2*aacK:2*aagH*aacD:6*acaM:9*gttR:2*caaS*aacD:1*gacN:2*tacF:1*gccS:1*ctcM:2*atgL*actS:3*gctS:2*gatN:3*ctgV:2*gctS:2*tccR:1*gacT*agaS*aagE:3*aagV:2*gctS:3*ttgI*gttI:9*attL*acgI:3*aggK:1*attL*cacY*cgcK:1*acgS*ggaE:3*gcaV*ttgI*gaaA:2*agaK*gaaD:1*tatF*gagD*tctV:2*gccR:2*tacF:9*cgcK:3*tccT:1*attV:6*gaaS*acaS*gcaP:1-aacctc:1*tctL*aaaQ:2*agtE:1*gtgP*gaaQ*agtG-gaa*agaF:1*gccL*tcaV:1*caaE*actR:1*gtcS*gctV*acaS*ccaN*gttS*ccaE:1-ccaatc:1*gaaN*cagI:1*actR*atgS*ggtS:1*ttcI:1-cagccaattgca:5+AM:2*ttcS*tggF*aacV*accR:2*aacQ*gtgE*tttL:1*gccE*gaaQ:1*cagR*agaQ*cttA*gcaE*agaE*gaaR*gccR:1*gaaA*aacE*atgL:1*caaE:2*caaM+L:1*gctR*gagD:1*cagA:1*attF:1-ttt:1*cagE:2*gctR*gctQ:2*gaaV*cagS:1*aggE:3*gaaS:3*cgcA:2*ttgM:1*aacR:2*gagQ:1*agaK*gtcL:1*gagQ:2*cgtQ:3*gcaT*atgL*aggG*caaG:1*tacH:1*aacR:3*ttaM:1*cagE:7*accV:2*cagN:1*attL*gccR*accI*acaV:4*actE*cagE:2*ggtA*tctV:1*gatT*gagS*cttQ*ttgC*aacS*agtH:1*cagE:2*ttgV*actQ*ttaY:5*gatE:17*aacG:3*aagI*ttaV:9*gccS:1*gagD*cgtK:9*tctA:2*gttI:3*ggtD:2*agaK:9*gagA*aaaR:3*ggtN*aaaD*gacS*agcE*gagQ*attV*gatQ*aagM:1*gagR*ctcT:1*atcL:2*ctgM*acaE*ttgT:2*gatK*gacS*tctL:1*agaE*agcT*cagH:1*tcgQ:2*tcgA:1*gtgL:1*gctS*tctQ:1*aagS*agaK:1*atgI:1*gacE*ttgI:2*aaaS*cttA+S:1*acaL*cagK*tctE*cgtG*gctG:2-ccagaccttgaacagaagctc*aaaE:1*aagA*gaaQ*gagQ:1*cttV*gagQ*attM*gtgL:2*accA:3*gccT*cttM:4-agagtccaggga:1*aacQ*gatH*gagQ:2*aacE*gaaK*caaE*cttA*gatE-gcagtcctgattgatcacctc:1*aagR*ctcV*acaL*tcaR:1*gttI:1*gccT*atcL:1*gctK*tccV*ggtS*atcS*aaaD:1*attL*cagR*gacE*tccF*ttgV*tacF*gagN:1*gacE:2*atgV*cagY*gctS*ggtN*aatT*caaD*aacI*tcaP*tcaT*ccgA:3*cttG*tccT*atcM*atcL:1*aagR*gccV*caaS*tcgH*tccL*gctS:1*gacQ:1*gccS*aacT*tctT*ttcM*aatT:1*ttcY:3*ggtN*ccaM*aatK:1*gatE+S*cacE:1*gagG*atcL:1*agaK*tccA:1*aacI:1*ttcL:1*tctD:2*gccG*gatN*gttL*ctgF*ctcS:1*gccV:1*ggtA*attV*gtgL:1*cttD*gtgR:1*actD:1*agtV*gaaQ-tct*aatE*cagI:1*attV*gccE*gccG:1*agaK*tctD*accL:1*cagH*gaaM*tctV:1*gacL:1*cttF*atgE:1*ctcM:2*gatN*atcH:1*gaaL*ggaK*actL:1+I*gaaH:1*aaaR:1*gaaD*gttR:2*tccT:2*ttgI:4*aagV*cttI:1*gaaS:1*attM*gttQ*attV*gctV:1*aacV*cttF*gcaR*ccaD*agaP*tctG*gttR*ctcK*aagT*aacP*atcV*aatS*ggtS:1+E:2*gatY*gcaF*gttL:1*aacK:2*aacA*tctN*gccT*gctQ*tctA:1*atcV*acaE*tctK:2*gctG*catL:1*aagE*gatS*ctgM+S*cttT*aacR*tccH*tctK*ttcL*gatE*tcaV*tccQ*atcE:2+SL*gatE*atcV:1*aagE:2*ttaI:1*gctC:1*attS:1*gttI*accI:1*tcaA*atcV*gctK:2*attL*cagE:1*gccC:1*gagD*acaA:1*aatE:3*accS*aaaR*aatG*cagR:2*ggtM*accS:2*cagL:4*cacN:10*gctS:1*gctQ:2*gctY*tccA:1*aagG:3*ggtR*actM:5*agtA*tctG*aagT:1*accS*catS:11*gctS*tccA:5*gctS*gccS:4*gctS*acaD*ttcL:2*aagQ*acgS*cagH*gatL*acgN:2*aatE:2*aagR*actK:3*gccS:1*agaK*tctM:2*aacT*caaK:1*cagR*gatS*attL:1-aaccag*agaL*acgS*aatD*ggtE*ggtS*gatN*cagK*gatT*attV:1*ttcY:1*aagQ:2*aacA:3*aagR:1*atgA:1*atgL:10*aatS:2*gctS*tccH:10*tatF*ggaS:3*ggtE*gacE:1 +CP048715.1 miniprot mRNA 641761 644859 2424 + . ID=PF000007;Rank=1;Identity=0.4986;Positive=0.6942;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +CP048715.1 miniprot CDS 641761 644859 2424 + 0 Parent=PF000007;Rank=1;Identity=0.4986;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 187 129 184 + CP048715.1 3128646 631962 632127 108 165 0 AS:i:187 ms:i:187 np:i:42 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:55M cs:Z::3*acaR*tcaH:1*accP*cctE*gaaS:3*tttL:2*agaK*gtgA:2*gctV:2*actF:3*acgK:2*aaaE*gttR:2*gaaR*aagE:2*ctcI*acgS:12*atgA:1*aacS:1 +CP048715.1 miniprot mRNA 631963 632127 187 + . ID=PF000008;Rank=1;Identity=0.6545;Positive=0.7636;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 130 184 +CP048715.1 miniprot CDS 631963 632127 187 + 0 Parent=PF000008;Rank=1;Identity=0.6545;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT 130 184 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 294 0 294 - CP048715.1 3128646 628451 629339 522 894 0 AS:i:879 ms:i:879 np:i:221 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:9M4D89M1I99M1I95M cs:Z:*agcM*tccN:1*ccaD*tcaK:2*acaK:1-cacaaagtcgac*gtgI*acaA*gcaP*cgcS*cagA*aagN*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgK*atcV:1*aaaG*ccaD*actS:1*ttcV*cagS*atgL:1*aagF*tctK:1*ttgI*gttK*catT*gatE:1*ctcV:1*ggtA*gttA:1*tctA:2*tctT:1*ggtS:8*ctcT*acaA:6*tatK:3*aatI*gttM*gttT+D:1*gctT*gatR*aagG:1*tcaA:1*atgT*gcaF*tacQ:2*cctA*gttA:2*ttgI*tctA:1*ggtA*attV:9*atcV:8*acaN*ggtS:1*gccP*ccaE*gatS:3*aacH:4*atcL:2*gtcL*atcL*agaK*gtgI*gctT:3*ggaN*actL*tctT:7*actP:2*gtgA:2*gtgI:3*tcgA:4*accS:5*gacK*tggL:1*ataV+E*tccN*ttcL*ggtS:1*aacD*aagP*gacK*tctT*attK*tccA:1*ttcH:5*cttI*tcaA:6*attV:18*gagQ:1*gtcS*gttF*cacG:2*agcK*accD:1*ttcI*aaaS*aacR:2*cctL*tctG:6*gttT:1*agtA:1*gttI:4*aatH:2*atcL:2*ttgI*gttA*gttL:1*cagE:1*aagR*cacR:1*cgtK*gttI:1*atgL +CP048715.1 miniprot mRNA 628449 629339 879 - . ID=PF000009;Rank=1;Identity=0.5839;Positive=0.7416;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +CP048715.1 miniprot CDS 628449 629339 879 - 0 Parent=PF000009;Rank=1;Identity=0.5839;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +CP048715.1 miniprot stop_codon 628449 628451 0 - 0 Parent=PF000009;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 305 0 305 - CP048715.1 3128646 628451 629357 519 915 0 AS:i:865 ms:i:859 np:i:226 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ctcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*acaP:1*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*cagT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:3*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*accS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggaE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:1*agcT:2*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aatH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +CP048715.1 miniprot mRNA 628449 629357 859 - . ID=PF000010;Rank=1;Identity=0.5672;Positive=0.7410;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 1 305 +CP048715.1 miniprot CDS 628449 629357 859 - 0 Parent=PF000010;Rank=1;Identity=0.5672;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 1 305 +CP048715.1 miniprot stop_codon 628449 628451 0 - 0 Parent=PF000010;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1045 0 1045 + CP048715.1 3128646 641751 644868 1824 3156 0 AS:i:2985 ms:i:2985 np:i:800 fs:i:0 st:i:0 da:i:6 do:i:0 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D51M3D4M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tccA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tctN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcgE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*aacR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aaaR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ctgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*atcV:1*ggtD*gatN*cttV:1*gatE*gcaL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:9*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*accN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gctY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE:2*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH-gatggtgac*agtA:3 +CP048715.1 miniprot mRNA 641752 644871 2985 + . ID=PF000011;Rank=1;Identity=0.5779;Positive=0.7605;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +CP048715.1 miniprot CDS 641752 644871 2985 + 0 Parent=PF000011;Rank=1;Identity=0.5779;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +CP048715.1 miniprot stop_codon 644869 644871 0 + 0 Parent=PF000011;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1044 0 1044 + CP048715.1 3128646 641751 644856 1815 3144 0 AS:i:2981 ms:i:2981 np:i:797 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D54M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tccA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tctN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcgE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*aacR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aaaR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ctgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*atcV:1*ggtD*gatN*cttV:1*gatE*gcaL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:9*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*accN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gctY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE:2*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +CP048715.1 miniprot mRNA 641752 644856 2981 + . ID=PF000012;Rank=1;Identity=0.5773;Positive=0.7605;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +CP048715.1 miniprot CDS 641752 644856 2981 + 0 Parent=PF000012;Rank=1;Identity=0.5773;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 305 0 305 - CP048715.1 3128646 628451 629357 519 915 0 AS:i:865 ms:i:859 np:i:226 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ctcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*acaP:1*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*cagT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:3*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*accS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggaE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:1*agcT:2*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aatH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +CP048715.1 miniprot mRNA 628449 629357 859 - . ID=PF000013;Rank=1;Identity=0.5672;Positive=0.7410;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 1 305 +CP048715.1 miniprot CDS 628449 629357 859 - 0 Parent=PF000013;Rank=1;Identity=0.5672;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 1 305 +CP048715.1 miniprot stop_codon 628449 628451 0 - 0 Parent=PF000013;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 305 0 305 - CP048715.1 3128646 628451 629357 519 915 0 AS:i:865 ms:i:859 np:i:226 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ctcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*acaP:1*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*cagT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:3*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*accS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggaE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:1*agcT:2*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aatH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +CP048715.1 miniprot mRNA 628449 629357 859 - . ID=PF000014;Rank=1;Identity=0.5672;Positive=0.7410;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 1 305 +CP048715.1 miniprot CDS 628449 629357 859 - 0 Parent=PF000014;Rank=1;Identity=0.5672;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 1 305 +CP048715.1 miniprot stop_codon 628449 628451 0 - 0 Parent=PF000014;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1044 0 1044 + CP048715.1 3128646 641751 644856 1815 3144 0 AS:i:2981 ms:i:2981 np:i:797 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D54M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tccA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tctN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcgE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*aacR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aaaR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ctgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*atcV:1*ggtD*gatN*cttV:1*gatE*gcaL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:9*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*accN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gctY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE:2*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +CP048715.1 miniprot mRNA 641752 644856 2981 + . ID=PF000015;Rank=1;Identity=0.5773;Positive=0.7605;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +CP048715.1 miniprot CDS 641752 644856 2981 + 0 Parent=PF000015;Rank=1;Identity=0.5773;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 287 20 287 - CP048715.1 3128646 628451 629246 492 801 0 AS:i:838 ms:i:838 np:i:207 fs:i:0 st:i:0 da:i:129 do:i:0 cg:Z:71M1I99M1I95M cs:Z::18*ccgK*atcV:1*aaaG*ccaD*actS:1*ttcV*cagS*atgL:1*aagY*tctK:1*ttgV*gttK*catT*gatE:1*ctcV:1*ggtA*gttA:1*tctA:4*ggtS:8*ctcT*acaA:6*tatK:2*gagG*aatI*gttM*gttT+D:1*gctT*gatK*aagG:1*tcaA:1*atgT*gcaF*tacQ:2*cctA*gttA:2*ttgI*tctA:1*ggtA*attV:9*atcV:8*acaN*ggtS:1*gccP*ccaE*gatS:3*aacH:4*atcL:2*gtcL*atcL*agaK*gtgI*gctT:3*ggaN*actI*tctT:7*actP:2*gtgA:2*gtgI:3*tcgA:4*accS:5*gacK*tggL:1*ataV+E*tccN*ttcL*ggtS:1*aacD*aagP*gacK*tctT*attK*tccA:1*ttcH:5*cttI*tcaA:6*attV:18*gagQ:1*gtcS*gttF*cacG:2*agcK*accD:1*ttcI*aaaS*aacR:2*cctL*tctG:6*gttT:1*agtA:1*gttI:4*aatH:2*atcL:2*ttgI*gttA*gttL:1*cagE:1*aagR*cacR:1*cgtK*gttI:1*atgL +CP048715.1 miniprot mRNA 628449 629246 838 - . ID=PF000016;Rank=1;Identity=0.6142;Positive=0.7753;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 21 287 +CP048715.1 miniprot CDS 628449 629246 838 - 0 Parent=PF000016;Rank=1;Identity=0.6142;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 21 287 +CP048715.1 miniprot stop_codon 628449 628451 0 - 0 Parent=PF000016;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 181 123 178 + CP048715.1 3128646 631962 632127 108 165 0 AS:i:187 ms:i:187 np:i:42 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:55M cs:Z::3*acaR*tcaH:1*accP*cctE*gaaS:3*tttL:2*agaK*gtgA:2*gctV:2*actF:3*acgK:2*aaaE*gttR:2*gaaR*aagE:2*ctcI*acgS:12*atgA:1*aacS:1 +CP048715.1 miniprot mRNA 631963 632127 187 + . ID=PF000017;Rank=1;Identity=0.6545;Positive=0.7636;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 124 178 +CP048715.1 miniprot CDS 631963 632127 187 + 0 Parent=PF000017;Rank=1;Identity=0.6545;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT 124 178 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 440 0 440 + CP048715.1 3128646 643515 644859 591 1362 0 AS:i:849 ms:i:849 np:i:296 fs:i:0 st:i:0 da:i:90 do:i:9 cg:Z:15M4D11M7D70M1I34M1D30M1I40M1I37M3I140M2D57M cs:Z:*gagQ*attM*gtgL:2*accA:3*gccT*cttM:4-agagtccaggga:1*aacQ*gatH*gagQ:2*aacE*gaaK*caaE*cttA*gatE-gcagtcctgattgatcacctc:1*aagR*ctcV*acaL*tcaR:1*gttI:1*gccT*atcL:1*gctK*tccV*ggtS*atcT*aaaD:1*attL*cagR*gacE*tccF*ttgV*tacF*gagN:1*gacE:2*atgV*cagY*gctS*ggtN*aatT*caaD*aacI*tcaP*tcaT*ccgA:3*cttG*tccT*atcM*atcL:2*gccV*caaS*tcgH*tccL*gctS:1*gacQ:1*gccS*aacT*tctT*ttcM*aatT:1*ttcY:3*ggtN*ccaM*aatR:2+S*cacE:1*gagG*atcL:1*agaK*tccA:1*aacT:3*tctD:2*gccG*gatN*gttL*ctgF*ctcS:1*gccV:1*ggtA*attV*gtgL:1*cttD*gtgR:1*actD:1*agtV*gaaQ-tct*aatE*cagI:1*attV*gccE*gccG*tccA*agaK*tctD*accL:1*cagH*gaaM*tctV:1*gacL:1*cttF*atgE:1*ctcM:3*atcH:1*gaaL*ggaK*actL:1+I*gaaH:1*aaaR:1*gaaD*gttR:2*tccT:2*ttgI:4*aagV*cttI:1*gaaS:1*attM*gttQ*attV*gctV:1*aacV*cttF*gcaR*ccaD*agaP*tctG*gttR*ctcK*aagS*aacP*atcV*aatS*ggtS:1+E:2*gatY*gcaF*gttL:1*aacK:2*aacA*tctN*gccT*gctQ*tctA:1*atcV*acaE*tctK:2*gctD*catL:1*aagE*gatS*ctgM*cttS*aacT*tccR*tctH*ttcK*gatL*tcaE*tccV*atcQ*gacE:1+GSL*gatE*atcV:1*aagE:2*ttaI:1*gctC:1*attS:1*gttI*accI:1*tcaA*atcV*gctK:2*attL*cagE:1*gccC:2*acaA:1*aatE:3*accS*aaaR*aatG*cagK:2*ggtL*accS:2*cagL:4*cacN:10*gctS:1*gctQ:2*gctY*tccA:1*aagG:3*ggtR*actM:5*agtA*tctG*aagT:1*accS*catS:11*gctS*tccA:5*gctS*gccS:4*gctS*acaD*ttcL:2*aagQ*acgS*cagH*gatL*acgN:2*aatE:2*aagR*actK:3*gccS:1*agaK*tctM:2*aacT*caaK:1*cagR*gatS*attL:1-aaccag*agaL:1*aatD*ggtE*ggtS*gatN*cagK*gatT*attV:1*ttcY:1*aagQ:2*aacA:3*aagR:1*atgA:1*atgL:10*aatS:2*gctS*tccQ:10*tatF*ggaS:3*ggtE*gacE:1 +CP048715.1 miniprot mRNA 643516 644859 849 + . ID=PF000018;Rank=1;Identity=0.4339;Positive=0.6520;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +CP048715.1 miniprot CDS 643516 644859 849 + 0 Parent=PF000018;Rank=1;Identity=0.4339;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 671 0 671 + CP048715.1 3128646 641751 644868 840 2229 0 AS:i:1044 ms:i:1075 np:i:392 fs:i:0 st:i:0 da:i:6 do:i:0 cg:Z:94M2I11M12I73M6I2M2I90M6I11M18I10M4I2M11I2M4I18M6I30M2D30M2I39M22D45M7D12M1D15M312U6D25M21D14M7D27M798N9M6D38M cs:Z:*aagM*acaS*atcR*tccE*gacE*tccQ*gacT:1*attE*tcaK:1*ttgI:3*actL:1*ctcG*gacG*gaaD*actV:20*agaK*cagT:3*cagL:3*ctcQ:1*gttY*cagA*ggtT:2*gttL:4*acaA:1*atcV*accL*attL:8*ggcP:3*aagV:1*gccG*atgI:2*gctI:4*ggcS:1*ggaS:1+IF:2*catD*ggtS:1*ggaD*ttcG*aagS*ggcK:2+SSRMRYIGDEYA*agaK*ctcA:1*aagG:3*gtcF*tatF:1*ttaV*agaE:2*aacQ:3*aacS:1*cgtK:4*acaI:10*accS:1*caaS*aacD:1*gacN:4*gccT*attV:4*actN:1*caaE:1*gctV*ctgA:1*gatN:2*agaK:4*agtG:2*catN*gacR*agaK+SGFGSF*aagS:1+GL*gagD:1*aagR*atcG*tctA*gctV:1*gtgI:3*gccS:1*tcaT:3*tacF:2*attV:3*ctcM*aggK:1*attL*cacY*cgcK*tccQ*acgM:1*tctD*gatY*gagD:2*gaaS*cctG:1*agaF*gaaD:2*gagT*tctE:1*catC:3*tacN:3*gctF:2*tccN*tcaT*atcV*cgcQ*tacP:1*actK*tccN:5*agaT:2*gaaS*acaD*gcaP*ccaM*aacS*ctcR:1*tctI*aaaD:2*agtD:1*gtgA:1+VNDVVF*agtE*gaaK:1*ccgS*gccK:1*agaS*caaV*actS:1*gtcL+PSRTPTRENLRDPSPVHE:1*acaP:2*ccaA*gaaR:1*atcA*tcgE*gaaV+RSLS:2+AAPVVPIATGV*actV:1+PVVT:1*aacY*ttcS:1*cagP:1*attL*gcaQ*tctV*cagV:3*gttA*gatE:2*aacS+IPSTSA*accP*caaT*caaR*aacS:1*tttV*gaaA*gccS*gaaH*cagD*cagD*agaL:1*gcaI*agaE*gaaQ*gccQ*caaK*gaaQ*aacQ*atgQ*cttI:4*cagY:1*gagL:1-cagcgt*attL*tggE*tttH*gaaQ*cagK:1*cagL*gctQ*gctL:1*gctQ:3*aggA:1*gccL:1*gaaQ*cagR*cttI*ttgQ*cgcQ*gatQ*cagE*ttgM*cagE*aacK*cagE:1+LI*gagQ*ggaQ*agaK*gtcT:1*gagS:1*gaaQ*cgtN:1*ttgI*ctcM*gcaS*atgF*aggK*caaN:3*aacR:2*ttgV*ttaM:1*cagE:3*caaK:2*aaaQ*accK:2*cagS:1*attL-gccaccacaacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcag:1*cagS*ttgV*actK*ttaE*tggF:1*aatE:2*gatQ:15*ctgI:1*aacK:1*tttM:2*ttaT:1*caaD*aagD*gctL:1*tcgE:1*cagE*gaaS*gccE*atcV-gagcgtcgtgagaagcttgag:1*gacK:3*aagS*aacA*gttL*gagS*ctgN*gcaV:1-ctg:2*gaaN*cgtN*gacN*agaK*gctG*agaE*ttgS*gacE*ttaR:1*aaaE*attA:1*gD~gt309ag-tc-ctgattgatcacctcact*aagR*ctcS*acaG*tcaM*ttgI*gttL*gatK*gccS*atcL*ttgV*gctE:1*ggtS:2*aggS*attL:2*tccP*ttgI*tacA*gagS:2-tctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaag*gccV*caaS*tcgD*tccL:3*tttL*gccS:1*tctA:1*aatI:1-ttcattgccgatggtccaaat:2*cacI*tctN*gagT:1*atcV*agaN*tccI*attL*aacP:2*acaV:1*tctL*atcL*gccL*gatR*gttC*ctgP*ctcQ*aatE*gccM*aagQ*ggtT:1~gt798ag*gatA*attE:2*tcgL*aagR*ttgC*acgL*aacS-catgagaacaagactatg*gagS*atgA*gagK:1*caaT*gtgD*gagS*attV*ttgI*aagN*ttgA*gagN*aatI:1*ttgF*gctQ*tccE*gctK*agaL:7*aagQ:1*ggaS:2*gatY:5*gagV +CP048715.1 miniprot mRNA 641752 644871 1075 + . ID=PF000019;Rank=1;Identity=0.3769;Positive=0.5276;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 671 +CP048715.1 miniprot CDS 641752 643300 987 + 0 Parent=PF000019;Rank=1;Identity=0.4085;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 557 +CP048715.1 miniprot CDS 643610 643911 21 + 2 Parent=PF000019;Rank=1;Identity=0.2000;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 558 624 +CP048715.1 miniprot CDS 644710 644871 67 + 0 Parent=PF000019;Rank=1;Identity=0.3585;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 625 671 +CP048715.1 miniprot stop_codon 644869 644871 0 + 0 Parent=PF000019;Rank=1 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 228 150 212 + CP048715.1 3128646 631944 632130 102 186 0 AS:i:178 ms:i:178 np:i:45 fs:i:0 st:i:0 da:i:-1 do:i:3 cg:Z:62M cs:Z:*cgaQ:1*gcgC*cttY:1*gcaT*aagY:2*acaS*tcaF:1*accK*cctH:2*aagR*gagG*tttI:2*agaK*gtgI:2*gctV:1*aggQ*actC:2*agtT*acgS:2*aaaL*gttY:2*gaaQ:1*tgtL*ggaD:3*ctgS:3*gtaI:8*aacA:1*gaaK +CP048715.1 miniprot mRNA 631945 632130 178 + . ID=PF000020;Rank=1;Identity=0.5484;Positive=0.7258;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 151 212 +CP048715.1 miniprot CDS 631945 632130 178 + 0 Parent=PF000020;Rank=1;Identity=0.5484;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT 151 212 +##PAF 4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 126 0 126 + CP048715.1 3128646 578876 632127 147 393 0 AS:i:193 ms:i:221 np:i:78 fs:i:0 st:i:0 da:i:-1 do:i:6 cg:Z:7M7I9M6I34M5D7M52900U55M cs:Z:*tccM*tccD:1*ctcI:1*gtaS*ataM+AENINRT:3*cttI*tggL:2*tctE*cttI+DEINLN*cgcT:1*gaaN*tgtL*ggcY*aagN*ggcF:1*ttgM:1*acgS*accN*gacL:3*gatE*aagQ*atcH:1*atcL*agcH:1*gagN*cttI*gtgS*aagN*gaaN*accR*ataL*aacE*ctcI*aggY*gatH-cgtcgaagggccacc:1*gttI*catK:1*cacE*gacK*ggcS*tP~gt52897ag-ca:1*aagG*cgaK*acaS:2*accS:1*gaaQ*tccA*aagR*gagA:3*agaQ:2*aagR*gctR:1*aggQ*actS*ccaL:2*acgK:1*aggK*aaaE*gttE*ataV:1*gaaK:3*ctcI:9*acaI:5*aacS:1 +CP048715.1 miniprot mRNA 578877 632127 221 + . ID=PF000021;Rank=1;Identity=0.3740;Positive=0.5954;Target=4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 1 126 +CP048715.1 miniprot CDS 578877 579063 37 + 0 Parent=PF000021;Rank=1;Identity=0.2237;Target=4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 1 70 +CP048715.1 miniprot CDS 631961 632127 184 + 2 Parent=PF000021;Rank=1;Identity=0.5818;Target=4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT 71 126 +##PAF 1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1064 0 1064 + CP048715.1 3128646 641736 644868 1803 3210 0 AS:i:2845 ms:i:2845 np:i:778 fs:i:0 st:i:0 da:i:-1 do:i:0 cg:Z:6M2D95M1D172M4I12M5I23M2D265M9I24M3I157M1I38M1I40M3I201M1D5M cs:Z:*cttM*gaaA*aagT*atgL:2-acaatc*tccA*gacE*tccA*gacE:1*attT*tcaV*tcgH*ttgI:4*tctN*ctcP*gacE:10*gcaQ:2*gtcI:4*cacN*aagH:1*tccG:1*cagA:1*ttcW:1*cagG*ctgM:1*ctgI*ctcQ:1*gttL:1*ggtN:4*ctcV:2*acaA:12*ggcP:1*gctV:1*aagR:2*atgQ*cgtQ:1*gctR*gatS:1*atcL:1*ggcS:1*ggaA:1*tcaG*gtcA:1*ggtA-gat:1*ttcR*aagH*ggcT:1*ggaA*agaK:2*aagY:3*gtcG*tatL:2*agaK:8*cgtP*ggaD:10*gtcI:2*gttR*accN*gtgI*caaD*aacD:1*gacN:3*gagQ:2*ctcV:3*actD:4*ctgI:1*gatN*cttF:4*tttI:2*atcL*tccK:1*gacG:1*aagP*tctN:2*aagR:4*gtgI:10*aagR:1*attT:1*tctT:4*attM:1*cgcA*tccT:1*ggaN*tctD:1*gagD:6*gaaS:2*gagY:3*gccQ:2*tacL:3*gctY:4*atcL*cgcK:4*ttgV:4*agaT:1*ccaS*gaaQ*acaD*gcaP:3*atcY*tctG*aaaE*gatN*gagP*agtE*gacE*gtgG:1*agtA+PALP*gaaK:2*gccI:1*agaE*caaV*actA:1*gtcL:2+GATTA:1*gttS*ccaT*gaaT*ccaD*atcL*tcgT*gaaP*cagE:1*actV*atgV*ggtD*aacF*ttcW*caaS*cagT:1*attQ*gcaG*tctQ:1*ccaQ-actggt*gttL*gatQ*ttcQ*tggQ*aacQ*accL:2*aacQ*gtgQ*tttY:1*gccE:2*cagR:3*agaA*gaaQ*gccR*caaE*gaaA*aacE*atgL*cttQ:3*caaM*cagL*gctH*gagL:2*cgtQ*attQ*tggQ:2*cagE:2*gctR*gctM:10*cagA:5*ttgY:1*aacR:2*gagD:2*gtcA:6*ttgI:1*gcaN*atgL:1*caaG:3*aacR:3*ttaM:1*cagE:3*caaR:3*accG:2*cagG:1*attL*gccQ*accQ*acaV*acaI*cagA*actN*gctT*actN*cagL:1*cttS*ggtQ:3*gagD*cttM*ttgI*aacR:5*ttgV*actN*ttaS*tggL:1*aatS:3*tctV:13*aacD*ctgK:1*aacK*aagE*tttL:1*aagL:1*cagK*caaV:10*cgtK:5*aagR:1*ttgI:1*tctA:2*gttI:3*ggtD:9*ttgY:2*gagD:1*attS*aagR:1*ggtN*aaaQ*gacK*agcD*gagV:1*gatE:3*ctcR:1*atcL:1*gacL*ctgT*acaE*ttgD:2*gatA:1*tctA:3*cagK*tccG*tcgA*aacD:2*tcgL*gtgL*tttI*gctS*tctK:1*aagN:2*atgL*gaaA:2*aagE*gcaE*aaaA:1*gagK*acaA*cagK*tctQ*cgtS*gctV:1+DNYANRSFD*aacD*ccaS:2*gaaK*cagL:2*aaaA:2*gaaD*gagD:4*gtgL:1*caaE:1*atgV:1*gatS+MEA:2*cagK:1*cttM*gccS*agaL*gtcN*cagH*ggaN:1*aacA:1*gagQ:1*attL*aacE:2*cttI:1*gcaE:2*attK*gatE:2*actS:2*acaN:1*ttgI*gttI:1*gccS*atcV:1*gctE:1*ggtS*atcA*aaaE:1*attV:1*gacE:2*tacF:1*ctgF*gacE:10*tcaT:7*atcV:3*caaS*tcgT:6*aacT:3*gacN:2*gccI:6*cacY:1*gagG:2*agaS*tccT*attA*aacT*gaaI*ttcL*acaS:1*tctV:1*gccP:3*ctcN:4*attL*gtgT:2*gtgA*aagR*actD:1*agtT*gaaV*tctA*aatD*cagN*ctcI:1*gccS*gccY*tccA:1*tctL*accA:1*cagV*gaaA*tctV:3*cttF*atgS*tctG*ctcV*ctcQ:1*gatN+K*atcL*cttA*gaaQ*ggaL*actS*gagD:1*gagD:2*gaaD*gttK:2*tccN*ggtF:1*ttgI*aacD:2*gagT*aagH:2*gaaS:1*attS*gttQ*attV*gctS:1*aacK:1*gcaT:2*tctA+G*gttT*ctcH*aagL:1*atcG*aatS:5*gcaL:2*aacR:1*ttgM*aacA*tctR:2*tctE:2*acaE:2*gctN:1*catK:1*aagN*gatE:2*aacS*tccR*tctP*ttcK:1+PKF:1*tccV*atcF:1*ggtL*gatK:1*aacH*aagE:3*tctA:6*aacT*tcaA:2*cttA:2*cagK:2*attS*gagD*acaC:1*aatQ:3*accA*aaaQ*aatG*cagK:1*agtA*ggtS*accS:1*gccT*cagA:17*gctS:5*aagN*atcV:2*ggtE:7*tctG*aagN*aacH:1*catP:1*gaaQ:9*gctS:13*acaS*ttcY:6*acgR:2*aatG:3*actA:16*aacS*cagK*agaK*acgQ*aatQ*ggtK*ggtE*gatE*cagN:1*attV:1*ttcY:4*aacP:1*gagV*aacL:2*atgT:13*gccS:1*gctN:12*ggaS:1*agaV:1-ggt:1*agtE:3 +CP048715.1 miniprot mRNA 641737 644871 2845 + . ID=PF000022;Rank=1;Identity=0.5617;Positive=0.7271;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +CP048715.1 miniprot CDS 641737 644871 2845 + 0 Parent=PF000022;Rank=1;Identity=0.5617;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +CP048715.1 miniprot stop_codon 644869 644871 0 + 0 Parent=PF000022;Rank=1 +##PAF 4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1053 0 1046 + CP048715.1 3128646 641745 644865 1644 3192 0 AS:i:2626 ms:i:2626 np:i:763 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:99M4I179M1I13M3I14M6D6M4I14M2I16M2D226M5I22M1D23M8D133M1I34M1D32M4I211M cs:Z::2*aagS*acaN*atcR*tccA*gacE*tccV:2*attN*tcaV*tcgN*ttgI:4*tctN*ctcT:2*actS:14*tggH:2*aagR:2*agaK*cagA:1*ttcW:1*cagG*ctgI*aaaR*ctgI*ctcQ:1*gttL:1*ggtT:1*gaaD:1*caaM*ctcV:2*acaA:12*ggcP:3*aagR*gagD:1*atgQ*cgtA:1*gctV*gatN*tggF:1*gagQ*ggcS:1*ggaA:1*tcaG*gtcN*catN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aagS:3*gtcR*tatF:1*ttaM*agaQ:2*aacT:3*aacY:1*cgtP*ggaA:3*acaM:4*gagD:1*gtcI:2*gttR:2*caaN*aacD:1*gacN:6*ctcM*gatN:6*gctG*ctgV:2*cttF:3*atcV:1*gctS:1*atcL*tccQ*catR*gacS:1*aagS*tctN:5*gctS:3*ttgM*gttI:7*aagR:1*attV*acgI:5*attM:1*cgcV:3*tctN:5*cctS:2*gaaQ:1*tatF*gagY:3*gccD:2*tacR*gagD:2*gctY:3*tcaT*atcL*cgcK:2*actR:1*ttgI*attV:3*agaQ:1*ccaG*gaaK*acaN*gcaP:3*atcL*tctE*aaaE*gatE*gagD*agtG*gacP*gtgG*gaaL*agtP*gaaQ:2*gccK:1+V*agaA*caaE:1*tcgR*gtcS*gctV*acaS:1*gttE:1*gaaA:1*atcL+PVA*tcgT*gaaP*cagT:1*actS*atgI*ggtP*aacA*ttcE*caaA:3*gcaE-tctcagccaactggtgtt*gatN:2*aacS*accQ*caaD+ALLA:1*aacQ*gtgQ*tttY*gaaD:3*cagE:2*gcaR*agaQ*gaaQ+QA*gccM*caaE:1*aacE*atgR*cttI*caaR:1*cagM:1*cagM*gctQ*gagQ:2*cgtQ-atttgg:2*cagM:2*gctR*gctQ:1*gctM:1*cagA:1*aggQ*ctaR:5*ttgM*cgcA:2*ttgM*cagA*aacR*cagH:1*gagQ:2*gtcM:6*ttgI:2*atgL:1*caaG:2*gagD*aacQ:3*ttaM:1*cagE:3*caaG:3*accA:2*cagA:1*attL*gccN*accQ*acaL*acaQ:3*actH:1*caaS*cttA*ggtQ*tctA:2*gagD:1*ttgI*aacE:3*gaaQ:1*ttgI:1*ttaM:1*aaaR*aatQ:2*gatE*tctT:3*ctgR:2*cagS*ttgM:1*cagE:1*catY:1*aacA:2*aacK:1*tttL:1*aagA*ttaT:7*cagK:4*cgtK*cgtA:4*aagR:1*ttgM*aagR*tctH:2*gttI:3*ggtD:2*agaK:6*ttgY:2*gagD*aaaR*attA:3*aaaN*gacK*agcE*gagD*attV*gatE*aagR:2*ctcR*cagE*atcL:1*gacM*ctgA*acaQ*ttgD:2*gatA:1*tctK*gagD:2*cagT*tccG*tcgA*aacD:2*tcgL*gtgL*tttL*gctS*tctK:1*aagN:2*atgL*gaaS*gacE:1*aagE*gcaN*aaaA:1*gagK*acaM*cagK*tctQ:3+DERGD*aacD*ccaS:2*gaaL*cagR*aagR:1*aaaE:3*gagI:1*cttY:1*attA*gtgL*cagN*caaE*accA*atgF-gat*gatN*gccS:1*cagA*gagL*cttH*gccQ*agaE*gtcQ:2*gagN:1*gatS*gagN*gctG*attG*aacS*gaaT*caaP:1*gatA:1-gtcctgattgatcacctcactaag:1*acaH:1*ttgI*gttI:2*atcL:1*gctE:2*atcS*aaaQ:1*attV:2*tccA:1*tacF:2*gacE:10*tcaT:6*atcV:4*caaS*tcgE:2*accS*gacA:2*aacT:3*gacN:1*attL:2*ggtE*ccaT*aatD:2*cacY*tctA:3*agaK*tccT*attV:1*gaaI*ttcY*acaS*tctT*tctA*atcV*gccE*gatN:2*ctcS:1*gccS:2*attL*gtgT:2*gtgA:1*actD:1*agtA*gaaS*tctA*aatD*cagA:1*attV*gccN*gccF*tccA:1*tctE*accS:1*cagE*gaaA*tctT:1*gacR*ttcN*cttF*atgI*tctG:3*gatE:1*cttI:1*ggaD*actF*gagP+L*gaaD*gagD:1*accM:1*gttR:2*tccT*ggtL:1*ttgM:3*gagT*aagA:2*gaaH:1*attT*gttK*attL:2*aacR*cttM:2*agaK*tctT*gttN*ctcI-aag:1*atcM*aatS:5*gcaL:1*gatE*aacR:1*ttgM*aacA*tctK:2*tctD:2*acaA*tctA:1*gctS:1*catK:1*aagG:3+KFNQ*aacS*tccD*tctP*ttcL*gatK:1*tccT*atcT:1*ggtL*gatQ*atcL*aacH*aagE:1*atcA*ttaI*tctQ:2*attQ:2*accI:1*tcaA:2*cttA:2*cagR:2*attT*gagD*acaA:5*accA*aaaQ*aatG*cagR:1*agtT*ggtS*accS:7*cacN:1*agaK:10*gctS:5*aagN:3*ggtE*actK:3*gtgT:1*agtR*tctR*aagT*aacS*accG*catL:8*gaaN:24*acgK:2*aatE*gctC:2*actV:6*tctN:2*aacK:3*gatE:3*cagK*agaK*acgF*aatG*ggtE*ggtL:1*cagE*gatK*attV:1*ttcY*tcgA*aagA:1*acgS*aacK:2*aacF:2*atgT:13*gccD:1*gctQ*tccG:6*gagQ*atcM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gatE:1 +CP048715.1 miniprot mRNA 641746 644865 2626 + . ID=PF000023;Rank=1;Identity=0.5150;Positive=0.7171;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +CP048715.1 miniprot CDS 641746 644865 2626 + 0 Parent=PF000023;Rank=1;Identity=0.5150;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +##PAF 4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1054 0 1047 + CP048715.1 3128646 641745 644865 1644 3195 0 AS:i:2615 ms:i:2615 np:i:763 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:99M4I179M1I13M3I14M6D6M4I14M2I16M2D226M5I22M1D23M8D133M1I34M1D25M1I7M4I211M cs:Z::2*aagS*acaN*atcR*tccA*gacE*tccV:2*attN*tcaV*tcgN*ttgI:4*tctN*ctcT:2*actS:14*tggH:2*aagR:2*agaK*cagA:1*ttcW:1*cagG*ctgI*aaaR*ctgI*ctcQ:1*gttL:1*ggtT:1*gaaD:1*caaM*ctcV:2*acaA:12*ggcP:3*aagR*gagD:1*atgQ*cgtA:1*gctV*gatN*tggF:1*gagQ*ggcS:1*ggaA:1*tcaG*gtcN*catN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aagS:3*gtcR*tatF:1*ttaM*agaQ:2*aacT:3*aacY:1*cgtP*ggaA:3*acaM:4*gagD:1*gtcI:2*gttR:2*caaN*aacD:1*gacN:6*ctcM*gatN:6*gctG*ctgV:2*cttF:3*atcV:1*gctS:1*atcL*tccQ*catR*gacS:1*aagS*tctN:5*gctS:3*ttgM*gttI:7*aagR:1*attV*acgI:5*attM:1*cgcV:3*tctN:5*cctS:2*gaaQ:1*tatF*gagY:3*gccD:2*tacR*gagD:2*gctY:3*tcaT*atcL*cgcK:2*actR:1*ttgI*attV:3*agaQ:1*ccaG*gaaK*acaN*gcaP:3*atcL*tctE*aaaE*gatE*gagD*agtG*gacP*gtgG*gaaL*agtP*gaaQ:2*gccK:1+V*agaA*caaE:1*tcgR*gtcS*gctV*acaS:1*gttE:1*gaaA:1*atcL+PVA*tcgT*gaaP*cagT:1*actS*atgI*ggtP*aacA*ttcE*caaA:3*gcaE-tctcagccaactggtgtt*gatN:2*aacS*accQ*caaD+ALLA:1*aacQ*gtgQ*tttY*gaaD:3*cagE:2*gcaR*agaQ*gaaQ+QA*gccM*caaE:1*aacE*atgR*cttI*caaR:1*cagM:1*cagM*gctQ*gagQ:2*cgtQ-atttgg:2*cagM:2*gctR*gctQ:1*gctM:1*cagA:1*aggQ*ctaR:5*ttgM*cgcA:2*ttgM*cagA*aacR*cagH:1*gagQ:2*gtcM:6*ttgI:2*atgL:1*caaG:2*gagD*aacQ:3*ttaM:1*cagE:3*caaG:3*accA:2*cagA:1*attL*gccN*accQ*acaL*acaQ:3*actH:1*caaS*cttA*ggtQ*tctA:2*gagD:1*ttgI*aacE:3*gaaQ:1*ttgI:1*ttaM:1*aaaR*aatQ:2*gatE*tctT:3*ctgR:2*cagS*ttgM:1*cagE:1*catY:1*aacA:2*aacK:1*tttL:1*aagA*ttaT:7*cagK:4*cgtK*cgtA:2*cttM:1*aagR:1*ttgM*aagR*tctH:2*gttI:3*ggtD:2*agaK:6*ttgY:2*gagD*aaaR*attA:3*aaaN*gacK*agcE*gagD*attV*gatE*aagR:2*ctcR*cagE*atcL:1*gacM*ctgA*acaQ*ttgD:2*gatA:1*tctK*gagD:2*cagT*tccG*tcgA*aacD:2*tcgL*gtgL*tttL*gctS*tctK:1*aagN:2*atgL*gaaS*gacE:1*aagE*gcaN*aaaA:1*gagK*acaM*cagK*tctQ:3+DERGD*aacD*ccaS:2*gaaL*cagR*aagR:1*aaaE:3*gagI:1*cttY:1*attA*gtgL*cagN*caaE*accA*atgF-gat*gatN*gccS:1*cagA*gagL*cttH*gccQ*agaE*gtcQ:2*gagN:1*gatS*gagN*gctG*attG*aacS*gaaT*caaP:1*gatA:1-gtcctgattgatcacctcactaag:1*acaH:1*ttgI*gttI:2*atcL:1*gctE:2*atcS*aaaQ:1*attV:2*tccA:1*tacF:2*gacE:10*tcaT:6*atcV:4*caaS*tcgE:2*accS*gacA:2*aacT:3*gacN:1*attL:2*ggtE*ccaT*aatD:2*cacY*tctA:3*agaK*tccT*attV:1*gaaI*ttcY*acaS*tctT*tctA*atcV*gccE*gatN:2*ctcS:1*gccS:2*attL*gtgT:2*gtgA:1*actD:1*agtA*gaaS*tctA*aatD*cagA:1*attV*gccN*gccF*tccA:1*tctE*accS:1*cagE*gaaA*tctT:1*gacR*ttcN*cttF*atgI*tctG:3*gatE:1*cttI:1*ggaD*actF*gagP+L*gaaD*gagD:1*accM:1*gttR:2*tccT*ggtL:1*ttgM:3*gagT*aagA:2*gaaH:1*attT*gttK*attL:2*aacR*cttM:2*agaK*tctT*gttN*ctcI-aag:1*atcM*aatS:5*gcaL:1*gatE*aacR:1*ttgM*aacA*tctK:2*tctD:2*acaA*tctA:2+S:1*catK:1*aagG:3+KFNQ*aacS*tccD*tctP*ttcL*gatK:1*tccT*atcT:1*ggtL*gatQ*atcL*aacH*aagE:1*atcA*ttaI*tctQ:2*attQ:2*accI:1*tcaA:2*cttA:2*cagR:2*attT*gagD*acaA:5*accA*aaaQ*aatG*cagR:1*agtT*ggtS*accS:7*cacN:1*agaK:10*gctS:5*aagN:3*ggtE*actK:3*gtgT:1*agtR*tctR*aagT*aacS*accG*catL:8*gaaN:24*acgK:2*aatE*gctC:2*actV:6*tctN:2*aacK:3*gatE:3*cagK*agaK*acgF*aatG*ggtE*ggtL:1*cagE*gatK*attV:1*ttcY*tcgA*aagA:1*acgS*aacK:2*aacF:2*atgT:13*gccD:1*gctQ*tccG:6*gagQ*atcM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gatE:1 +CP048715.1 miniprot mRNA 641746 644865 2615 + . ID=PF000024;Rank=1;Identity=0.5146;Positive=0.7164;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +CP048715.1 miniprot CDS 641746 644865 2615 + 0 Parent=PF000024;Rank=1;Identity=0.5146;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 diff --git a/results/2026-10-30_petasospora/data/GCA_050575385.1_ASM5057538v1.miniprot.gff b/results/2026-10-30_petasospora/data/GCA_050575385.1_ASM5057538v1.miniprot.gff new file mode 100644 index 00000000..b44c14eb --- /dev/null +++ b/results/2026-10-30_petasospora/data/GCA_050575385.1_ASM5057538v1.miniprot.gff @@ -0,0 +1,72 @@ +##gff-version 3 +##PAF MH260578_SUI1 109 0 109 - CM115473.1 3009164 613879 614206 267 327 0 AS:i:488 ms:i:488 np:i:103 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:109M cs:Z::2*tctN:1*cagE:2*aacK*actS:10*gatE:1*cagE*ccaV:30*agaK:2*cgtK:1*ttgI:2*gacE:24*gtcL:1*gttI:3*ttgM*accV*tccQ*cagT:1*gccG*ttgI*cagK:9 +CM115473.1 miniprot mRNA 613877 614206 488 - . ID=PF000001;Rank=1;Identity=0.8165;Positive=0.9450;Target=MH260578_SUI1 1 109 +CM115473.1 miniprot CDS 613877 614206 488 - 0 Parent=PF000001;Rank=1;Identity=0.8165;Target=MH260578_SUI1 1 109 +CM115473.1 miniprot stop_codon 613877 613879 0 - 0 Parent=PF000001;Rank=1 +##PAF MH260578_SLA2 1072 29 1072 + CM115473.1 3009164 648055 651157 1704 3156 0 AS:i:2754 ms:i:2754 np:i:774 fs:i:0 st:i:0 da:i:21 do:i:0 cg:Z:94M2D73M1D86M1I24M3I24M2I256M5I54M4D129M1I41M3I39M3I150M2D55M cs:Z:*tctT:1*cttI*attS*tcaT*tcgA*ttgI:3*actC*tctT*ctcP*gacE*gaaD:2*ccaS:18*agaK*cagD:1*ttcW*aacE*cagT*ctgM:1*ctgV*ctcV:1*gttL*cagK*ggtN*gatT*gaaD*gttT:4*acaA:2*accL:9*ggcP:3*aagV*gagG*gccG*atgF*cgtK:1*gctM*gatE:2*gagS*ggcN:1*ggaD*agaT*tcaL*gtcR*catT*ggtL:1-ggattc*aagR*ggcR*tacF*ggaQ*agaV:2*aagP:3*gtcN:1*ttgI:1*agaQ:2*aacR:2*aagH:17*gttR:2*caaS*aacD:1*gacN:3*gagD:5*atgT*actS:3*gctS:2*gatS*cttF*caaG:1*ctgD:3*agtT*atcV-tcc*catR*gacN*agaN*aagN:3*aagI:6*ttgI:10*attL*acgI:5*attM*cacY*cgcK*tccM*acgS*ggaD:1*gatA:1*gcaI:4*agaK*gaaS*cgcK*tatF:1*tctD:2*gccE:3*gagD:3*gacN:2*tcaN:5*tccT:5*agaK:2*gaaF*acaE:4+I:1*tccQ*aaaN:2*agtD*gacE*gtgN*gaaV*agtN*gaaL*agaP:1*gccL*tcaT*agaE:1*actK*tcgT*gtcA:2*ccaT*gttL+RHS:1*gaaT:1*atcF*tcgE*gaaQ*cagE*ccaN*actE*atgR*ggtQ*aacQ*ttcQ:1*cagA:3*tctT:4*gttM+VR*gatN*ttcA*tggF*aacV*accE:2*aacQ*gtgL*tttY:1*gccQ*gaaQ:2*agaQ:1*gcaE*agaQ*gaaQ*gccR:1*gaaA*aacE*atgL*cttQ:5*gctQ*gagL*caaA:1*cgtQ*attQ*tggY*tttW:4*gctR*gctQ:3*cagA:1*aggQ:3*gaaQ:3*cgcL:2*ttgA:1*aacR*cagH:1*gagQ:9*ttgI:1*gcaT*atgL*aggK*caaG:2*gagD:4*ttaM:2*cagS:3*agaK:1*aagQ*accA:2*cagN:1*attL:1*accS:5*actQ*cagE:2*ggtA:4*cttQ:2*agtL:4*ttgV*actE*ttaY:5*gatE:10*cagT:6*aacT:1*tttS:2*ttaI:12*cgtK:5*aagR:1*ttgM:1*tctA:2*gttI:3*ggtD:2*agaK:7*gacE:2*aaaR*attF:1*ggtN:1*aaaT*gacK*agcT*gagD*attL*gatQ*aagT:1*gagQ:1*cagE*atcK*aggD*gacE:1*acaE*ttgQ:2*gatA*gacT*tctL*gagD:1*agcA:2*tcgA:2*tcgT*tcgA*gtgI:1*gctN*tctQ:1*aagN*agaK:1*atgL*gaaQ*gacE:1*aagQ*gcaN:1+FKDVT:1*gagN*acaA*cagP:1*cgtD*gctE*ctgK*aacL*ccaK*gacQ:2*cagE*aagQ:1*aaaQ:2*gaaD*gagL:1*cttI:1*attM*gtgM:5*gatE*gccT*cttI*cagK*gagD:1*gccV*agaK*gtcT:1*ggaN*gagS*aacR*gatE:1*gctK*attS*aacE*gaaM:1*cttN*gatK*gcaL-gtcctgattgat*cacN*ctcS:2*ctcV*acaL*tcaG:1*gttI:4*gctK:3*aaaN:4*tccA*ttgV*tacF:10*caaL:6*ttgV:1*tccT*atcL*atcL:2*gccS*caaS*tcgG*tccL*gctV:4*aacS:3*gacN*ttcM*attL*gccV:2*ccaD:1*ggtA*gatN*cacE*tctA*gagL*atcV:1*agaD:2*aacT*gaaN:2*tctT:2*gccS:1*gttI:3*gccT:2*attL*gtgT:2*gtgT:1*actQ:1*agtD*gaaF*tctQ*aatD*cagD:2*gccD*gccT*tccA*agaK*tctD*accI:1*cagE*gaaI:2*gacV*ttcY:1*atgE:2*ctcY+H:1*gatN*atcL*cttV*gaaN*ggaK*actS*gagI*gaaD*gagD*aaaQ:1*gaaD*gttI:2*tccN:2*ttgI*aacD:3*aagI:2*gaaT:1*attL*gttQ*attL*gctV:1*aacS*cttM*gcaK*ccaT*agaP:1*gttS+KID:1*aagS*aacK*attV*aatD:1*gatE:1*ggtS:1*gctL:2*aacK:1*ttgM*aacE*tctH:1*gctS*tctR*gctV:1*acaE*tctE:1*gctS*gctV:2*aagS*gatS:3*tccQ*tctP:2+VSM:4*ggtI*gatE*atcV:2*gccS:2*tctA:2*attS*tccA*gttI*accI*aacT*tcaA:1*gctK*cttY:2*cagR:1*gccS:2*acaS:1*aatE:3*acgN*aaaN*aatG*cagR:2*ggtH*accS*cgtK*gccT*cagS:4*cacN:1*agaK:8*gctS:1*gctS*gttI:1*gccY:2*aagN*atcT:2*ggtR*actI:5*agtQ*tctN:1*aacH*accS*catN:3*attV:3*aatR:10*gctS:1*tcaA:3*gctS*acaQ:6*gatN*acgK:2*aatD:3*actK:1*actN*tctL:2*agaK*tctQ:2*aacD:2*cagN*gatK*attL*ctgI*aacI*cagG*agaK*acgH*aatE-ggtggt*gatV*cagE:3*ttcY:3*acgS*aacI:5*atgA:15*gctS:11*tatF*ggaS:1*agaK:1*ggtD:5 +CM115473.1 miniprot mRNA 648056 651160 2754 + . ID=PF000002;Rank=1;Identity=0.5399;Positive=0.7357;Target=MH260578_SLA2 30 1072 +CM115473.1 miniprot CDS 648056 651160 2754 + 0 Parent=PF000002;Rank=1;Identity=0.5399;Target=MH260578_SLA2 30 1072 +CM115473.1 miniprot stop_codon 651158 651160 0 + 0 Parent=PF000002;Rank=1 +##PAF MH260578_TGL1 440 12 440 + CM115477.1 1737500 377298 378573 585 1314 0 AS:i:911 ms:i:911 np:i:269 fs:i:0 st:i:0 da:i:27 do:i:51 cg:Z:15M2I11M2D4M5D23M1D51M4I229M7I20M1D29M1D33M cs:Z:*tcgA*ctcV:1*gagQ*tggL:2*gtcL*gtgI*gccF*tcgH*ttcK:1*gctG:1+EE:2*gagS*tttY*gtaF*atcQ*tccT*cttP*atcS*accN:1-gccatc:2*tcaN*gtgI-ctcgacctcgttaca:1*ttcA*atcV*caaS:1*gtgE*cacN*tctK:1*atcK*gttI*ccaS*gaaP*aagS*atcA:1*aacP*ggtL:1*ataE*ctcV*gagA:1-gct*gccT*aagH*aagC*atcL*acaH*aagQ*gatK:1*aggI:2*cctN*tcaD:1*caaV*cacD:1*ggaV:1*ctcA*tttH:2*caaK*attV*gatH*gatE:3*cctQ:1*gcaK:5*tgtS*cttI:3*aggL*ccaK*ctcS*aagS*gaaG*ggcD*gcaK:1+QNSR:9*atgT*tgcN:1*gatE*atcL*tggF*gttL*gtcL*aacG*atcE*aagN:2*agaK*aatC:3*attL:1*catV:1*atgQ:1*tttY:3*atgL:9*acaR:3*tccF:1*aaaS*ccaS*tcaT*catD*aagE*agaK:2*gacN*ttcY:1*atcL:5*tatF:3*gatN*tccT*atcV:4*tcaR*tacN:1*aagQ:1*caaD*gacS:1*acaS:10*gcaC*tttL:5*catN*gatH*aaaD:2*accK*cagK:1*aagN*ctcK:2*gctG:1*gctS:3*acaI:1*agtQ:2*cacN:1*caaP*ttcI*gtcA*gatS*acaF:1*atgV*aaaT:1*acaA:1*aacG*ttcL*atgL:1*ctgR*gccI:4*ctcA*ctcI:2*acaS*gcaV*tcaV:2*aaaQ*aatK*atcL*tgtM*tacG:1*ccaV:1*ttcY*tctE:1*atcV*atcV:1*cttK:1*gttL*cgcV*atgF:2*gacN:2*aatS*tacG:2*actK*catT*gatS:2*attK*gctV*gcaG*ttcY*tccP:1*ctcM*tacF:2*acaS:5*gttA:6*cgcD*aatS*ggtK*gcaR:2*ttgM:3*gacG+GASGSRL:1*tcaY:1*accS*aatG*gccT*ctcS*tctG*gctK*ccaT*ttcN:1*aacV:7-aat:1*aaaT*gtaT:1*atcC*aagL*ttgF*attV:2*tcaK*attS:1*tccM:1*gtgI:2*gatE*aagR*atgT:1*tcaD*ttaN:1*ccaS*gctC:2-aca*gatQ*gctT*gtaI*gcaG:1*ccaD*aatG*catY:2*ttgM:1*atcT*atcL:1*ggtA:1*cacQ*attV:2*ctcK:3*catV*ttgV*ttcL*aatQ*ttcY:1*ggtK*gtcQ +CM115477.1 miniprot mRNA 377299 378573 911 + . ID=PF000003;Rank=1;Identity=0.4452;Positive=0.6142;Target=MH260578_TGL1 13 440 +CM115477.1 miniprot CDS 377299 378573 911 + 0 Parent=PF000003;Rank=1;Identity=0.4452;Target=MH260578_TGL1 13 440 +##PAF MH260578_TGL1 440 79 440 - CM115477.1 1737500 1548097 1549159 525 1101 0 AS:i:881 ms:i:881 np:i:237 fs:i:0 st:i:0 da:i:54 do:i:45 cg:Z:90M1I91M1I86M11I10M3D34M3D37M cs:Z::1*tttI*gcaV*tctD*cttI*gctV*gcaH:1*caaH:2*cagK:1*caaH:2*actI:3*tccK:5*actS*ctcI:2*gtcI*tttL*gaaK*aagS*gaaS*gttG*tccD*tgcK*cagP*aagQ*ggcN*aaaS*cctR:1*gctV:1*ttcL:11*atcL:2*gacE*cgcN*cgtK*gatE:4*tatF:2*ttgV*gagD*aagQ:15+R*tccK:1*atcL*acaF:1*actS*gagS*tctT:1*ccaE*cgcK*tacF:1*gacN*ttcY:6*cacM*acaF:3*tctN*attT*atcV:4*gacR*ttcN:1*ggcQ:1*ccaD:2*actS:7*acaS*acaA:1*atgC*tttL:2*atgL:1*attL:1*gagH:3*tctK:1*gtcI:1*ttcK:1*gttI*gctG:3*gtgA:4*ccaG:1*actN*cacN:1*ctaI*atcA*aagS*ccaF*ttcL:1+T*caaS*actA:1*tcaG*aatL*atcL*accY*aagR*gtgI:2*agcK*ggaR*cctA*ttaI:2*ttcS:1*aatV*cttF*gtcW*aatQ:1*gccL*atcM*ccgG*cttP*cctV:2*aagE*accK*atcV*attV:1*gtcK:2*ggtV*atcF:3*accW*tcaK*ggaS*gccG:2*agcK*tcgT*aagS:2*ctcK*gcaV:3*tacH*ttgM:2*gttP:4*tgtS:1*gtaA:6*agaD*cagS*cagK*tccR:1*cacQ*aagM*tacF+DEGGASGSRLV:1*aaaL*gctS*gctG*gatT:1*aaaG*atgK:1*gatN-gacctctgg*gagR:1*gagA*ctcS:1*gatP*atgT*aaaQ*aatT*ctgI*aaaT*gtgT:3*ttgF:3*gacK*gctS:1*atcM:1*gtcI:2*gatE*aagR:1*aggL*cagD*catN*ttcL-aagcagtca*aacS*ctgC:5*aagG*gtgI*agtD:11*gagQ:1*ttgE*tatK*acgK:2*gcaP*cctV:2*gagQ*aagY*attL*gaaK*gagQ +CM115477.1 miniprot mRNA 1548098 1549159 881 - . ID=PF000004;Rank=2;Identity=0.4768;Positive=0.6458;Target=MH260578_TGL1 80 440 +CM115477.1 miniprot CDS 1548098 1549159 881 - 0 Parent=PF000004;Rank=2;Identity=0.4768;Target=MH260578_TGL1 80 440 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1019 7 1019 + CM115473.1 3009164 648049 651148 1557 3129 0 AS:i:2421 ms:i:2421 np:i:724 fs:i:0 st:i:0 da:i:15 do:i:9 cg:Z:256M2D10M1D15M2D10M4D5M2I31M1I8M1D218M1I8M7D22M4D11M7D70M1I34M1D30M1I40M1I26M1I11M2I140M2D57M cs:Z::1*gacN*tctL:1*cttI*attQ*tcaA:5*actC*tctT*ctcA:1*gaaA*actA:21*cagE:1*ttcW*aacH*cagC:5*gttI:1*ggtS*gatN*gaaD*gttT:1*ctcI:5*accV:9*ggcP*tctT*gctC:1*aagI*gagG*gccG*atgY*cgtK:1*gctI*gatS:1*atcL:1*ggcS:1*ggaS:1*tcaF*gtcS*catN*ggtN*gatG*ggaT*ttcA*aagA:2*ggaT:7*gtcF:2*ttaE*agaQ:2*aacK:2*aagH*aacD:6*acaM:9*gttR:2*caaS*aacD:1*gacN:2*tacF:1*gccS:1*ctcM:2*atgL*actS:3*gctS:2*gatN:3*ctgV:2*gctS:2*tccR:1*gacT*agaS*aagE:3*aagV:2*gctS:3*ttgI*gttI:9*attL*acgI:3*aggK:1*attL*cacY*cgcK:1*acgS*ggaE:3*gcaV*ttgI*gaaA:2*agaK*gaaD:1*tatF*gagD*tctV:2*gccR:2*tacF:9*cgcK:3*tccT:1*attV:6*gaaS*acaS*gcaP:1-aacctc:1*tccL*aaaQ:2*agtE:1*gtgP*gaaQ*agtG-gaa*agaF:1*gccL*tcaV:1*caaE*actR:1*gtcS*gctV*acaS*ccaN*gttS*ccaE:1-ccaatc:1*gaaN*cagI:1*actR*atgS*ggtS:1*ttcI:1-cagccaattgca:5+AM:2*ttcS*tggF*aacV*accR:2*aacQ*gtgE*tttL:1*gccE*gaaQ:1*cagR*agaQ*cttA*gcaE*agaE*gaaR*gccR:1*gaaA*aacE*atgL:1*caaE:2*caaM+L:1*gctR*gagD:1*cagA:1*attF:1-ttt:1*cagE:2*gctR*gctQ:2*gaaV*cagS:1*aggE:3*gaaS:3*cgcA:2*ttgM:1*aacR:2*gagQ:1*agaK*gtcL:1*gagQ:2*cgtQ:3*gcaT*atgL*aggG*caaG:1*tacH:1*aacR:3*ttaM:1*cagE:7*accV:2*cagN:1*attL*gccR*accI*acaV:4*actE*cagE:2*ggtA*tctV:1*gatT*gagS*cttQ*ttgC*aacS*agtH:1*cagE:2*ttgV*actQ*ttaY:5*gatE:17*aacG:3*aagI*ttaV:9*gccS:1*gagD*cgtK:9*tctA:2*gttI:3*ggtD:2*agaK:9*gagA*aaaR:3*ggtN*aaaD*gacS*agcE*gagQ*attV*gatQ*aagM:1*gagR*ctcT:1*atcL:2*ttgM*acaE*ttgT:2*gatK*gacS*tctL:1*agaE*agcT*cagH:1*tcgQ:2*tcgA:1*gtgL:1*gctS*tctQ:1*aagS*agaK:1*atgI:1*gacE*ttgI:2*aaaS*cttA+S:1*acaL*cagK*tctE*cgtG*gctG:2-ccagaccttgaacagaagctc*aaaE:1*aagA*gaaQ*gagQ:1*cttV*gagQ*attM*gtgL:2*accA:3*gccT*cttM:4-agagtccaggga:1*aacQ*gatH*gagQ:2*aacE*gaaK*caaE*cttA*gatE-gcagtcctgattgatcacctc:1*aagR*ctcV*acaL*tcaR:1*gttI:1*gccT*atcL:1*gctK*tccV*ggtS*atcS*aaaD:1*attL*cagR*gacE*tccF*ttgV*tacF*gagN:1*gacE:2*atgV*cagY*gctS*ggtN*aatT*caaD*aacI*tcaP*tcaT*ccgA:3*cttG*tccT*atcM*atcL:1*aagR*gccV*caaS*tcgH*tccL*gctS:1*gacQ:1*gccS*aacT*tctT*ttcM*aatT:1*ttcY:3*ggtN*ccaM*aatK:1*gatE+S*cacE:1*gagG*atcL:1*agaK*tccA:1*aacI:1*ttcL:1*tctD:2*gccG*gatN*gttL*ctgF*ctcS:1*gccV:1*ggtA*attV*gtgL:1*cttD*gtgR:1*actD:1*agtV*gaaQ-tct*aatE*cagI:1*attV*gccE*gccG:1*agaK*tctD*accL:1*cagH*gaaM*tctV:1*gacL:1*cttF*atgE:1*ctcM:2*gatN*atcH:1*gaaL*ggaK*actL:1+I*gaaH:1*aaaR:1*gaaD*gttR:2*tccT:2*ttgI:4*aagV*cttI:1*gaaS:1*attM*gttQ*attV*gctV:1*aacV*cttF*gcaR*ccaD*agaP*tctG*gttR*ctcK*aagT*aacP*attV*aatS*ggtS:1+E:2*gatY*gctF*gttL:1*aacK:2*aacA*tctN*gccT*gctQ*tctA:1*atcV*acaE*tctK:2*gctG*catL:1*aagE*gatS*ctgM+S*cttT*aacR*tccH*tctK*ttcL*gatE*tcaV*tccQ*atcE:2+SL*gatE*atcV:1*aagE:2*ttaI:1*gctC:1*attS*tccA*gttI*accI:1*tcaA*atcV*gctK:2*attL*cagE:1*gccC:1*gagD*acaA:1*aatE:3*acgS*aaaR*aatG*cagR:2*ggtM*accS:2*cagL:4*cacN:10*gctS:1*gctQ:2*gccY*tccA:1*aagG:3*ggtR*actM:5*agtA*tctG*aagT:1*accS*catS:11*gctS*tccA:5*gctS*gccS:4*gctS*acaD*ttcL:2*aagQ*acgS*cagH*gatL*acgN:2*aatE:2*aagR*actK:3*gccS:1*agaK*tctM:2*aacT*caaK:1*cagR*gatS*attL:1-aaccag*agaL*acgS*aatD*ggtE*ggtS*gatN*cagK*gatT*attV:1*ttcY:1*aagQ:2*aacA:3*aagR:1*atgA:1*atgL:10*aatS:2*gctS*tccH:10*tatF*ggaS:3*ggtE*gacE:1 +CM115473.1 miniprot mRNA 648050 651148 2421 + . ID=PF000005;Rank=1;Identity=0.4976;Positive=0.6942;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +CM115473.1 miniprot CDS 648050 651148 2421 + 0 Parent=PF000005;Rank=1;Identity=0.4976;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved 8 1019 +##PAF 1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 294 0 294 - CM115473.1 3009164 631648 632536 525 894 0 AS:i:886 ms:i:886 np:i:222 fs:i:0 st:i:0 da:i:36 do:i:0 cg:Z:9M4D89M1I99M1I95M cs:Z:*agcM*tccN:1*ccaD*tcaK:2*acaK:1-cacaaagtcgac*gtgI*acaA:1*cgcS*cagA*aagN*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgK*atcV:1*aaaG*ccaD*actS:1*ttcV*caaS*atgL:1*aagF*tctK:1*ttgI*gttK*catT*gatE:1*ctcV:1*ggtA*gttA:1*tctA:2*tctT:1*ggtS:8*ctcT*acaA:6*tatK:3*aatI*gttM*gttT+D:1*gctT*gatR*aagG:1*tcaA:1*atgT*gcaF*tacQ:2*cctA*gttA:2*ttgI*tctA:1*ggtA*attV:9*atcV:8*acaN*ggtS:1*gccP*ccaE*gatS:3*aacH:4*atcL:2*gtcL*atcL*agaK*gtgI*gctT:3*ggaN*actL*tctT:7*actP:2*gtgA:2*gtgI:3*tcgA:4*actS:5*gacK*tggL:1*ataV+E*tccN*ttcL*ggtS:1*aacD*aagP*gacK*tctT*attK*tccA:1*ttcH:5*cttI*tcaA:6*attV:18*gagQ:1*gtcS*gttF*cacG:2*accK*accD:1*ttcI*aaaS*aacR:2*cctL*tctG:6*gttT:1*agtA:1*gttI:4*aacH:2*atcL:2*ttgI*gttA*gttL:1*cagE:1*aagR*cacR:1*cgtK*gttI:1*atgL +CM115473.1 miniprot mRNA 631646 632536 886 - . ID=PF000006;Rank=1;Identity=0.5872;Positive=0.7450;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +CM115473.1 miniprot CDS 631646 632536 886 - 0 Parent=PF000006;Rank=1;Identity=0.5872;Target=1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved 1 294 +CM115473.1 miniprot stop_codon 631646 631648 0 - 0 Parent=PF000006;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 305 0 305 - CM115473.1 3009164 631648 632554 519 915 0 AS:i:863 ms:i:857 np:i:225 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ttcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*acaP*ccaA*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*caaT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:3*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*actS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggaE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:4*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aacH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +CM115473.1 miniprot mRNA 631646 632554 857 - . ID=PF000007;Rank=1;Identity=0.5672;Positive=0.7377;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 1 305 +CM115473.1 miniprot CDS 631646 632554 857 - 0 Parent=PF000007;Rank=1;Identity=0.5672;Target=460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved 1 305 +CM115473.1 miniprot stop_codon 631646 631648 0 - 0 Parent=PF000007;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1045 0 1045 + CM115473.1 3009164 648040 651157 1821 3156 0 AS:i:2982 ms:i:2982 np:i:800 fs:i:0 st:i:0 da:i:6 do:i:0 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D51M3D4M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tctA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tccN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcgE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*aacR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aaaR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ttgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*attV:1*ggtD*gatN*cttV:1*gatE*gctL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:6*tccA:2*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*acgN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gccY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE:2*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH-gatggtgac*agtA:3 +CM115473.1 miniprot mRNA 648041 651160 2982 + . ID=PF000008;Rank=1;Identity=0.5770;Positive=0.7605;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +CM115473.1 miniprot CDS 648041 651160 2982 + 0 Parent=PF000008;Rank=1;Identity=0.5770;Target=460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved 1 1045 +CM115473.1 miniprot stop_codon 651158 651160 0 + 0 Parent=PF000008;Rank=1 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1044 0 1044 + CM115473.1 3009164 648040 651145 1812 3144 0 AS:i:2978 ms:i:2978 np:i:797 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D54M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tctA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tccN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcgE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*aacR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aaaR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ttgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*attV:1*ggtD*gatN*cttV:1*gatE*gctL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:6*tccA:2*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*acgN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gccY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE:2*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +CM115473.1 miniprot mRNA 648041 651145 2978 + . ID=PF000009;Rank=1;Identity=0.5763;Positive=0.7605;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +CM115473.1 miniprot CDS 648041 651145 2978 + 0 Parent=PF000009;Rank=1;Identity=0.5763;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved 1 1044 +##PAF 460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 305 0 305 - CM115473.1 3009164 631648 632554 519 915 0 AS:i:863 ms:i:857 np:i:225 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ttcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*acaP*ccaA*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*caaT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:3*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*actS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggaE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:4*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aacH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +CM115473.1 miniprot mRNA 631646 632554 857 - . ID=PF000010;Rank=1;Identity=0.5672;Positive=0.7377;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 1 305 +CM115473.1 miniprot CDS 631646 632554 857 - 0 Parent=PF000010;Rank=1;Identity=0.5672;Target=460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved 1 305 +CM115473.1 miniprot stop_codon 631646 631648 0 - 0 Parent=PF000010;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 305 0 305 - CM115473.1 3009164 631648 632554 519 915 0 AS:i:863 ms:i:857 np:i:225 fs:i:0 st:i:0 da:i:18 do:i:0 cg:Z:110M1I4M1I93M1I95M cs:Z:*aagM:1*gagQ*agcI*accN*ttcR*agcE:1*tctN*ccaD*tcaD:2*acaM*tccQ*cacY*aaaE*gtcR*gacI*gtgP*acaP*ccaA*cgcK*cagG*aagV*tggF:6*ttcA*ggtA*ggtS*ggtM*gttF:18*ccgS*atcQ:1*aaaG*ccaQ*actN:1*ttcL*caaT*atgI:1*aagI*tctR:1*ttgI*gttS*catS*gatE:1*ctcI:2*gttA:6*ggtS:8*ctcT:4*gtgI:2*tatA:2*gagD*aatY*gttL*gttT*cctK:1+N*gatN*aagN:1*tcaD+L:1*atgT:1*tacI:3*gttC:3*tctA:5*gttL:4*gctS:1*atcV:8*acaS*ggtS:1*gccP*ccaK*gatP*cagA:4*aagR*catN:1*atcL*gatH:1*gtcL*atcA:1*gtgM:1*cgtK:3*actL*tctG*gctS*ctgW:4*ggaL*actP:2*gtgT:6*tcgA:4*actS:5*gacK*tggF:1*ataV+K*tccK*ttcL*ggtS*atgF*aacD*aagE*gacT*tctT*attR*tccA:1*ttcH:5*cttM*tcaA:6*attV:6*ctcV:7*tctA*ggaE*acaK*ggtH*gagQ:1*gtcI*gttI*cacT*atcV:4*ttcM*aaaR*aacK:3*tctG:1*atgL:4*gttF:3*gttI:4*aacH:2*atcL:2*ttgV*gttA*gttL:3*aagR*cacT*ttgW:1*gttI*ggtA:1 +CM115473.1 miniprot mRNA 631646 632554 857 - . ID=PF000011;Rank=1;Identity=0.5672;Positive=0.7377;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 1 305 +CM115473.1 miniprot CDS 631646 632554 857 - 0 Parent=PF000011;Rank=1;Identity=0.5672;Target=460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved 1 305 +CM115473.1 miniprot stop_codon 631646 631648 0 - 0 Parent=PF000011;Rank=1 +##PAF 460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1044 0 1044 + CM115473.1 3009164 648040 651145 1812 3144 0 AS:i:2978 ms:i:2978 np:i:797 fs:i:0 st:i:0 da:i:6 do:i:12 cg:Z:213M1I78M3D13M1I5M3I264M5I182M1I69M1I5M1I148M1D54M cs:Z:*aagM*acaS*atcR*tccQ*gacE*tctA:2*attK*tcaV:1*ttgI:3*actC:1*ctcT*gacE:1*actA:12*tacF:4*aagR:2*agaK*cagA:1*ttcY:1*cagG:1*aaaR:3*gttI:1*ggtN:2*gttI*caaP:3*acaS:12*ggcP:2*ttgI:2*gccG*atgI*cgtK:1*gctR:3*gagQ*ggcS:2*agaH*tcaV*gtcF*catP:3*ttcM:1*ggcR:5*aagR:2*gtgD*gtcR:2*ttaI:2*ctcI*aacD:2*aagN*aacS:1*cgtK:14*gttK:2*caaS*aacD:1*gacN:6*ctcM:3*actV:3*gctS*ctgI*gacN:5*atcL:4*tccD*catS*gacS*agaS*aagH:2*tgtL:8*gttI:6*tacF:8*gctG*attM*cacC*cgcH:1*acgL:1+H*tctD:4*gaaQ:2*agaI:2*tatF*gagI:2*catY*gccS*agaD:2*gagD:3*gacE:2*tcaY*atcV*cgcK:5*attV*acaN:2*agaK:2*gaaF*acaE:2*aacD:1*atcF*tccN*aaaW:2*agtT:1*gtgE*gaaL*agtK*gaaP:1*ccgI*gccL*tcaA*agaE*caaE*actP:1*gtcL*gctQ*acaV*ccaS*gttS*ccaK*gaaA:1*atcS*tcgE:1-cagccaact*atgF:1*aacT*ttcP*caaT*cagE*ccaV:1*gcaH:4+A:1*gttT:1*ttcL:1+AQS:1*accA*caaL*caaK*aacQ*gtgQ*tttY:1*gccQ:3*agaL:1*gcaE*agaQ:1*gccR:1*gaaQ*aacQ*atgL:1*caaE:2*caaH:1*gctQ*gagQ:2*cgtL*attE*tggY*tttW:1*cagK:1*cagK*gctE*gctL:1*gctE*gaaQ:2*aggL*ctaY:2*gaaQ:2*ttgE*cgcS:4*aacR:1*gcaV*gagQ:3*gcaG:7*gcaI*atgL:1*caaG:1*tacF*gagD*aacR:3*ttaM:1*cagR:3*caaE:3*accA:2*cagT:1*attL*gccS*accS:3*actN:1*actQ:4*tctA:1*gatE:3*aacG*agtN*ttgI:3*ttgA*actA*ttaQ:2*aatS:13*cagE:6*aacS:3*aagT*ttaA:1*caaL:2*gccS:2*cagR:3*gagD*cgtK:3*cttI:1*aagR:3*tctA:2*gttI:3*ggtD:13*aaaR*attS:1*ggtS*ggtD:1*gacN:2*attT*gatE*aagR:2*ctcA*cagR*atcL:1*gacE*ttgQ*acaE*ttgT:2*gatA:1*tctI:1*agaK*agcL*cagH:2*aacE:1*tcgK:1*gtgF*tttL*gctS*tctQ:1*aagN*agaK:1*atgI:1*gacE:1*aagQ*gcaN*aaaQ:1*gagQ*acaA*cagS:1*cgtL*gctS*ctgE*aacS*ccaA*gacN+ERLKA:2*cagA*aagQ:1*aaaQ:2*gaaD*gagD:2*gagA:1*gtgA*cagH:3*gatE*gatA*gccT*cttI*cagK:2*gccT*agaG*gtcQ:1*ggaS*gagI*aacQ*gatN*gagS:2*aacD:2*cttI:2*gtcI:1*attL*gatE:1*ctcI*actD:3*tcaE*ttgV*gttI:4*gctT:2*atcV*aaaQ:4*tccG:1*tacF:18*cttS:5*gccS*caaS*tcgN*tccL:2*gacE:1*gccT*aacS:3*gacG:10*tctV*gagS*atcV:1*agaQ*tccT:1*aacT:3*tctT:2*gccV:1*gttL:3*gccC:2*attV:3*gtgA*aagS*actS:1*agtQ:1*tctA*aatE*cagA:1*attL*gccN*gccS*tccA:1*tctD*accV:1*cagE:3*gacS:1*cttI*atgV:2*ctcY:1*gatS*atcS:1*gaaK:1+L*actS*gagD*gaaQ:3*gaaD*gttK*gttA:1*tccE:2*ttgV*aacD*gtcI:1*gagQ*aagE:2*gaaT:1*attS*gttS*attL*gctV:1*aacK:1*gcaI*ccaS*agaS*tctQ:1*ctcN*aagI:1*attV:1*ggtD*gatN*cttV:1*gatE*gctL:2*aacR:1*ttgM*aacA*tctH:2*tctE*gctT:1*acaA*tctK:1*gctS*gctD:2*aagT:3+T:1*tccA*tctK*ttcQ:1+I:1*tccT:2*ggtM*gatK:2*aagN*gccS:6*tccA:2*aacK*tcaA:1*gctI:3*cagK*gccT:1*attT*gagD*acaC:5*acgN*aaaH*aatG*cagR:2*ggtS*accS:1*gccT*cagS:6*agaK:8*gctS:1*gctS:2*gccY:2*aagN*atcS:2*ggtQ*actI:7*aagT*aacS*accS*catP:1*gaaQ:4*tccC:1*gaaD:16*acaS:7*acgK:2*aatL:3*actS:9*aacR:3*gatE:2*aacS:1*agaK-acg*aatD*ggtK*ggtE*gatE*cagL*gatE:2*ttcY:3*acgS*aacA:5*atgV*gagQ:11*aatT:2*gctQ*tccN:2*aagR:6*aagR*tatF:2*agaH*gatA*ggtD*gacE +CM115473.1 miniprot mRNA 648041 651145 2978 + . ID=PF000012;Rank=1;Identity=0.5763;Positive=0.7605;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +CM115473.1 miniprot CDS 648041 651145 2978 + 0 Parent=PF000012;Rank=1;Identity=0.5763;Target=460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved 1 1044 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 287 20 287 - CM115473.1 3009164 631648 632443 492 801 0 AS:i:837 ms:i:837 np:i:207 fs:i:0 st:i:0 da:i:129 do:i:0 cg:Z:71M1I99M1I95M cs:Z::18*ccgK*atcV:1*aaaG*ccaD*actS:1*ttcV*caaS*atgL:1*aagY*tctK:1*ttgV*gttK*catT*gatE:1*ctcV:1*ggtA*gttA:1*tctA:4*ggtS:8*ctcT*acaA:6*tatK:2*gagG*aatI*gttM*gttT+D:1*gctT*gatK*aagG:1*tcaA:1*atgT*gcaF*tacQ:2*cctA*gttA:2*ttgI*tctA:1*ggtA*attV:9*atcV:8*acaN*ggtS:1*gccP*ccaE*gatS:3*aacH:4*atcL:2*gtcL*atcL*agaK*gtgI*gctT:3*ggaN*actI*tctT:7*actP:2*gtgA:2*gtgI:3*tcgA:4*actS:5*gacK*tggL:1*ataV+E*tccN*ttcL*ggtS:1*aacD*aagP*gacK*tctT*attK*tccA:1*ttcH:5*cttI*tcaA:6*attV:18*gagQ:1*gtcS*gttF*cacG:2*accK*accD:1*ttcI*aaaS*aacR:2*cctL*tctG:6*gttT:1*agtA:1*gttI:4*aacH:2*atcL:2*ttgI*gttA*gttL:1*cagE:1*aagR*cacR:1*cgtK*gttI:1*atgL +CM115473.1 miniprot mRNA 631646 632443 837 - . ID=PF000013;Rank=1;Identity=0.6142;Positive=0.7753;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 21 287 +CM115473.1 miniprot CDS 631646 632443 837 - 0 Parent=PF000013;Rank=1;Identity=0.6142;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved 21 287 +CM115473.1 miniprot stop_codon 631646 631648 0 - 0 Parent=PF000013;Rank=1 +##PAF 460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 440 0 440 + CM115473.1 3009164 649804 651148 588 1362 0 AS:i:846 ms:i:846 np:i:296 fs:i:0 st:i:0 da:i:90 do:i:9 cg:Z:15M4D11M7D70M1I34M1D30M1I40M1I37M3I140M2D57M cs:Z:*gagQ*attM*gtgL:2*accA:3*gccT*cttM:4-agagtccaggga:1*aacQ*gatH*gagQ:2*aacE*gaaK*caaE*cttA*gatE-gcagtcctgattgatcacctc:1*aagR*ctcV*acaL*tcaR:1*gttI:1*gccT*atcL:1*gctK*tccV*ggtS*atcT*aaaD:1*attL*cagR*gacE*tccF*ttgV*tacF*gagN:1*gacE:2*atgV*cagY*gctS*ggtN*aatT*caaD*aacI*tcaP*tcaT*ccgA:3*cttG*tccT*atcM*atcL:2*gccV*caaS*tcgH*tccL*gctS:1*gacQ:1*gccS*aacT*tctT*ttcM*aatT:1*ttcY:3*ggtN*ccaM*aatR:2+S*cacE:1*gagG*atcL:1*agaK*tccA:1*aacT:3*tctD:2*gccG*gatN*gttL*ctgF*ctcS:1*gccV:1*ggtA*attV*gtgL:1*cttD*gtgR:1*actD:1*agtV*gaaQ-tct*aatE*cagI:1*attV*gccE*gccG*tccA*agaK*tctD*accL:1*cagH*gaaM*tctV:1*gacL:1*cttF*atgE:1*ctcM:3*atcH:1*gaaL*ggaK*actL:1+I*gaaH:1*aaaR:1*gaaD*gttR:2*tccT:2*ttgI:4*aagV*cttI:1*gaaS:1*attM*gttQ*attV*gctV:1*aacV*cttF*gcaR*ccaD*agaP*tctG*gttR*ctcK*aagS*aacP*attV*aatS*ggtS:1+E:2*gatY*gctF*gttL:1*aacK:2*aacA*tctN*gccT*gctQ*tctA:1*atcV*acaE*tctK:2*gctD*catL:1*aagE*gatS*ctgM*cttS*aacT*tccR*tctH*ttcK*gatL*tcaE*tccV*atcQ*gacE:1+GSL*gatE*atcV:1*aagE:2*ttaI:1*gctC:1*attS*tccA*gttI*accI:1*tcaA*atcV*gctK:2*attL*cagE:1*gccC:2*acaA:1*aatE:3*acgS*aaaR*aatG*cagK:2*ggtL*accS:2*cagL:4*cacN:10*gctS:1*gctQ:2*gccY*tccA:1*aagG:3*ggtR*actM:5*agtA*tctG*aagT:1*accS*catS:11*gctS*tccA:5*gctS*gccS:4*gctS*acaD*ttcL:2*aagQ*acgS*cagH*gatL*acgN:2*aatE:2*aagR*actK:3*gccS:1*agaK*tctM:2*aacT*caaK:1*cagR*gatS*attL:1-aaccag*agaL:1*aatD*ggtE*ggtS*gatN*cagK*gatT*attV:1*ttcY:1*aagQ:2*aacA:3*aagR:1*atgA:1*atgL:10*aatS:2*gctS*tccQ:10*tatF*ggaS:3*ggtE*gacE:1 +CM115473.1 miniprot mRNA 649805 651148 846 + . ID=PF000014;Rank=1;Identity=0.4317;Positive=0.6520;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +CM115473.1 miniprot CDS 649805 651148 846 + 0 Parent=PF000014;Rank=1;Identity=0.4317;Target=460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved 1 440 +##PAF 28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 671 0 671 + CM115473.1 3009164 648040 651157 840 2229 0 AS:i:1044 ms:i:1075 np:i:392 fs:i:0 st:i:0 da:i:6 do:i:0 cg:Z:94M2I11M12I73M6I2M2I90M6I11M18I10M4I2M11I2M4I18M6I30M2D30M2I39M22D45M7D12M1D15M312U6D25M21D14M7D27M798N9M6D38M cs:Z:*aagM*acaS*atcR*tccE*gacE*tctQ*gacT:1*attE*tcaK:1*ttgI:3*actL:1*ctcG*gacG*gaaD*actV:20*agaK*cagT:3*cagL:3*ctcQ:1*gttY*cagA*ggtT:2*gttL:4*acaA:1*atcV*accL*attL:8*ggcP:3*aagV:1*gccG*atgI:2*gctI:4*ggcS:1*ggaS:1+IF:2*catD*ggtS:1*ggaD*ttcG*aagS*ggcK:2+SSRMRYIGDEYA*agaK*ctcA:1*aagG:3*gtcF*tatF:1*ttaV*agaE:2*aacQ:3*aacS:1*cgtK:4*acaI:10*accS:1*caaS*aacD:1*gacN:4*gccT*attV:4*actN:1*caaE:1*gctV*ctgA:1*gatN:2*agaK:4*agtG:2*catN*gacR*agaK+SGFGSF*aagS:1+GL*gagD:1*aagR*atcG*tctA*gctV:1*gtgI:3*gctS:1*tcaT:3*tacF:2*attV:3*ctcM*aggK:1*attL*cacY*cgcK*tccQ*acgM:1*tctD*gatY*gagD:2*gaaS*cctG:1*agaF*gaaD:2*gagT*tctE:1*catC:3*tacN:3*gctF:2*tccN*tcaT*atcV*cgcQ*tacP:1*actK*tccN:5*agaT:2*gaaS*acaD*gcaP*ccaM*aacS*ctcR:1*tccI*aaaD:2*agtD:1*gtgA:1+VNDVVF*agtE*gaaK:1*ccgS*gccK:1*agaS*caaV*actS:1*gtcL+PSRTPTRENLRDPSPVHE:1*acaP:2*ccaA*gaaR:1*atcA*tcgE*gaaV+RSLS:2+AAPVVPIATGV*actV:1+PVVT:1*aacY*ttcS:1*cagP:1*attL*gcaQ*tctV*cagV:3*gttA*gatE:2*aacS+IPSTSA*accP*caaT*caaR*aacS:1*tttV*gaaA*gccS*gaaH*cagD*cagD*agaL:1*gcaI*agaE*gaaQ*gccQ*caaK*gaaQ*aacQ*atgQ*cttI:4*cagY:1*gagL:1-cagcgt*attL*tggE*tttH*gaaQ*cagK:1*cagL*gctQ*gctL:1*gctQ:3*aggA:1*gccL:1*gaaQ*cagR*cttI*ttgQ*cgcQ*gatQ*cagE*ttgM*cagE*aacK*cagE:1+LI*gagQ*ggaQ*agaK*gtcT:1*gagS:1*gaaQ*cgtN:1*ttgI*ctcM*gcaS*atgF*aggK*caaN:3*aacR:2*ttgV*ttaM:1*cagE:3*caaK:2*aagQ*accK:2*cagS:1*attL-gccaccacaacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcag:1*cagS*ttgV*actK*ttaE*tggF:1*aatE:2*gatQ:15*ctgI:1*aacK:1*tttM:2*ttaT:1*caaD*aagD*gctL:1*tcgE:1*cagE*gaaS*gccE*atcV-gagcgtcgtgagaagcttgaa:1*gacK:3*aagS*aacA*gttL*gagS*ctgN*gcaV:1-ctg:2*gaaN*cgtN*gacN*agaK*gctG*agaE*ttgS*gacE*ttaR:1*aaaE*attA:1*gD~gt309ag-tc-ctgattgatcacctcact*aagR*ctcS*acaG*tcaM*ttgI*gttL*gatK*gccS*atcL*ttgV*gctE:1*ggtS:2*aggS*attL:2*tccP*ttgI*tacA*gagS:2-tctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaag*gccV*caaS*tcgD*tccL:3*tttL*gccS:1*tctA:1*aatI:1-ttcattgccgatggtccaaat:2*cacI*tctN*gagT:1*atcV*agaN*tccI*attL*aacP:2*acaV:1*tctL*atcL*gccL*gatR*gttC*ctgP*ctcQ*aatE*gccM*aagQ*ggtT:1~gt798ag*gatA*attE:2*tcgL*aagR*ttgC*acgL*aacS-catgagaacaagactatg*gagS*atgA*gagK:1*caaT*gtgD*gagS*attV*ttgI*aagN*ttgA*gagN*aatI:1*ttgF*gctQ*tccE*gctK*agaL:7*aagQ:1*ggaS:2*gatY:5*gagV +CM115473.1 miniprot mRNA 648041 651160 1075 + . ID=PF000015;Rank=1;Identity=0.3769;Positive=0.5276;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 671 +CM115473.1 miniprot CDS 648041 649589 987 + 0 Parent=PF000015;Rank=1;Identity=0.4085;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 1 557 +CM115473.1 miniprot CDS 649899 650200 21 + 2 Parent=PF000015;Rank=1;Identity=0.2000;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 558 624 +CM115473.1 miniprot CDS 650999 651160 67 + 0 Parent=PF000015;Rank=1;Identity=0.3585;Target=28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved 625 671 +CM115473.1 miniprot stop_codon 651158 651160 0 + 0 Parent=PF000015;Rank=1 +##PAF 1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1064 0 1064 + CM115473.1 3009164 648025 651157 1800 3210 0 AS:i:2842 ms:i:2842 np:i:778 fs:i:0 st:i:0 da:i:-1 do:i:0 cg:Z:6M2D95M1D172M4I12M5I23M2D265M9I24M3I157M1I38M1I40M3I201M1D5M cs:Z:*cttM*gaaA*aagT*atgL:2-acaatc*tccA*gacE*tctA*gacE:1*attT*tcaV*tcgH*ttgI:4*tctN*ctcP*gacE:10*gcaQ:2*gtcI:4*cacN*aagH:1*tccG:1*cagA:1*ttcW:1*cagG*ctgM:1*ctgI*ctcQ:1*gttL:1*ggtN:4*ctcV:2*acaA:12*ggcP:1*gctV:1*aagR:2*atgQ*cgtQ:1*gctR*gatS:1*atcL:1*ggcS:1*ggaA:1*tcaG*gtcA:1*ggtA-gat:1*ttcR*aagH*ggcT:1*ggaA*agaK:2*aagY:3*gtcG*tatL:2*agaK:8*cgtP*ggaD:10*gtcI:2*gttR*accN*gtgI*caaD*aacD:1*gacN:3*gagQ:2*ctcV:3*actD:4*ctgI:1*gatN*cttF:4*tttI:2*atcL*tccK:1*gacG:1*aagP*tctN:2*aagR:4*gtgI:10*aagR:1*attT:1*tctT:4*attM:1*cgcA*tccT:1*ggaN*tctD:1*gagD:6*gaaS:2*gagY:3*gccQ:2*tacL:3*gctY:4*atcL*cgcK:4*ttgV:4*agaT:1*ccaS*gaaQ*acaD*gcaP:3*atcY*tccG*aaaE*gatN*gagP*agtE*gacE*gtgG:1*agtA+PALP*gaaK:2*gccI:1*agaE*caaV*actA:1*gtcL:2+GATTA:1*gttS*ccaT*gaaT*ccaD*atcL*tcgT*gaaP*cagE:1*actV*atgV*ggtD*aacF*ttcW*caaS*cagT:1*attQ*gcaG*tctQ:1*ccaQ-actggt*gttL*gatQ*ttcQ*tggQ*aacQ*accL:2*aacQ*gtgQ*tttY:1*gccE:2*cagR:3*agaA*gaaQ*gccR*caaE*gaaA*aacE*atgL*cttQ:3*caaM*cagL*gctH*gagL:2*cgtQ*attQ*tggQ:2*cagE:2*gctR*gctM:10*cagA:5*ttgY:1*aacR:2*gagD:2*gtcA:6*ttgI:1*gcaN*atgL:1*caaG:3*aacR:3*ttaM:1*cagE:3*caaR:3*accG:2*cagG:1*attL*gccQ*accQ*acaV*acaI*cagA*actN*gctT*actN*cagL:1*cttS*ggtQ:3*gagD*cttM*ttgI*aacR:5*ttgV*actN*ttaS*tggL:1*aatS:3*tctV:13*aacD*ctgK:1*aacK*aagE*tttL:1*aagL:1*cagK*caaV:10*cgtK:5*aagR:1*ttgI:1*tctA:2*gttI:3*ggtD:9*ttgY:2*gagD:1*attS*aagR:1*ggtN*aaaQ*gacK*agcD*gagV:1*gatE:3*ctcR:1*atcL:1*gacL*ttgT*acaE*ttgD:2*gatA:1*tctA:3*cagK*tccG*tcgA*aacD:2*tcgL*gtgL*tttI*gctS*tctK:1*aagN:2*atgL*gaaA:2*aagE*gcaE*aaaA:1*gagK*acaA*cagK*tctQ*cgtS*gctV:1+DNYANRSFD*aacD*ccaS:2*gaaK*cagL:2*aaaA:2*gaaD*gagD:4*gtgL:1*caaE:1*atgV:1*gatS+MEA:2*cagK:1*cttM*gccS*agaL*gtcN*cagH*ggaN:1*aacA:1*gagQ:1*attL*aacE:2*cttI:1*gcaE:2*attK*gatE:2*actS:2*acaN:1*ttgI*gttI:1*gccS*atcV:1*gctE:1*ggtS*atcA*aaaE:1*attV:1*gacE:2*tacF:1*ctgF*gacE:10*tcaT:7*atcV:3*caaS*tcgT:6*aacT:3*gacN:2*gccI:6*cacY:1*gagG:2*agaS*tccT*attA*aacT*gaaI*ttcL*acaS:1*tctV:1*gccP:3*ctcN:4*attL*gtgT:2*gtgA*aagR*actD:1*agtT*gaaV*tctA*aatD*cagN*ctcI:1*gccS*gccY*tccA:1*tctL*accA:1*cagV*gaaA*tctV:3*cttF*atgS*tctG*ctcV*ctcQ:1*gatN+K*atcL*cttA*gaaQ*ggaL*actS*gagD:1*gagD:2*gaaD*gttK:2*tccN*ggtF:1*ttgI*aacD:2*gagT*aagH:2*gaaS:1*attS*gttQ*attV*gctS:1*aacK:1*gcaT:2*tctA+G*gttT*ctcH*aagL:1*attG*aatS:5*gctL:2*aacR:1*ttgM*aacA*tctR:2*tctE:2*acaE:2*gctN:1*catK:1*aagN*gatE:2*aacS*tccR*tctP*ttcK:1+PKF:1*tccV*atcF:1*ggtL*gatK:1*aacH*aagE:3*tctA:3*tccA:2*aacT*tcaA:2*cttA:2*cagK:2*attS*gagD*acaC:1*aatQ:3*acgA*aaaQ*aatG*cagK:1*agtA*ggtS*accS:1*gccT*cagA:17*gctS:5*aagN*atcV:2*ggtE:7*tctG*aagN*aacH:1*catP:1*gaaQ:9*gctS:13*acaS*ttcY:6*acgR:2*aatG:3*actA:16*aacS*cagK*agaK*acgQ*aatQ*ggtK*ggtE*gatE*cagN:1*attV:1*ttcY:4*aacP:1*gagV*aacL:2*atgT:13*gccS:1*gctN:12*ggaS:1*agaV:1-ggt:1*agtE:3 +CM115473.1 miniprot mRNA 648026 651160 2842 + . ID=PF000016;Rank=1;Identity=0.5607;Positive=0.7271;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +CM115473.1 miniprot CDS 648026 651160 2842 + 0 Parent=PF000016;Rank=1;Identity=0.5607;Target=1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved 1 1064 +CM115473.1 miniprot stop_codon 651158 651160 0 + 0 Parent=PF000016;Rank=1 +##PAF 4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1053 0 1046 + CM115473.1 3009164 648034 651154 1641 3192 0 AS:i:2623 ms:i:2623 np:i:763 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:99M4I179M1I13M3I14M6D6M4I14M2I16M2D226M5I22M1D23M8D133M1I34M1D32M4I211M cs:Z::2*aagS*acaN*atcR*tccA*gacE*tctV:2*attN*tcaV*tcgN*ttgI:4*tctN*ctcT:2*actS:14*tggH:2*aagR:2*agaK*cagA:1*ttcW:1*cagG*ctgI*aaaR*ctgI*ctcQ:1*gttL:1*ggtT:1*gaaD:1*caaM*ctcV:2*acaA:12*ggcP:3*aagR*gagD:1*atgQ*cgtA:1*gctV*gatN*tggF:1*gagQ*ggcS:1*ggaA:1*tcaG*gtcN*catN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aagS:3*gtcR*tatF:1*ttaM*agaQ:2*aacT:3*aacY:1*cgtP*ggaA:3*acaM:4*gagD:1*gtcI:2*gttR:2*caaN*aacD:1*gacN:6*ctcM*gatN:6*gctG*ctgV:2*cttF:3*atcV:1*gctS:1*atcL*tccQ*catR*gacS:1*aagS*tctN:5*gctS:3*ttgM*gttI:7*aagR:1*attV*acgI:5*attM:1*cgcV:3*tctN:5*cctS:2*gaaQ:1*tatF*gagY:3*gccD:2*tacR*gagD:2*gctY:3*tcaT*atcL*cgcK:2*actR:1*ttgI*attV:3*agaQ:1*ccaG*gaaK*acaN*gcaP:3*atcL*tccE*aaaE*gatE*gagD*agtG*gacP*gtgG*gaaL*agtP*gaaQ:2*gccK:1+V*agaA*caaE:1*tcgR*gtcS*gctV*acaS:1*gttE:1*gaaA:1*atcL+PVA*tcgT*gaaP*cagT:1*actS*atgI*ggtP*aacA*ttcE*caaA:3*gcaE-tctcagccaactggtgtt*gatN:2*aacS*accQ*caaD+ALLA:1*aacQ*gtgQ*tttY*gaaD:3*cagE:2*gcaR*agaQ*gaaQ+QA*gccM*caaE:1*aacE*atgR*cttI*caaR:1*cagM:1*cagM*gctQ*gagQ:2*cgtQ-atttgg:2*cagM:2*gctR*gctQ:1*gctM:1*cagA:1*aggQ*ctaR:5*ttgM*cgcA:2*ttgM*cagA*aacR*cagH:1*gagQ:2*gtcM:6*ttgI:2*atgL:1*caaG:2*gagD*aacQ:3*ttaM:1*cagE:3*caaG:3*accA:2*cagA:1*attL*gccN*accQ*acaL*acaQ:3*actH:1*caaS*cttA*ggtQ*tctA:2*gagD:1*ttgI*aacE:3*gaaQ:1*ttgI:1*ttaM:1*aaaR*aatQ:2*gatE*tctT:3*ctgR:2*cagS*ttgM:1*cagE:1*catY:1*aacA:2*aacK:1*tttL:1*aagA*ttaT:7*cagK:4*cgtK*cgtA:4*aagR:1*ttgM*aagR*tctH:2*gttI:3*ggtD:2*agaK:6*ttgY:2*gagD*aaaR*attA:3*aaaN*gacK*agcE*gagD*attV*gatE*aagR:2*ctcR*cagE*atcL:1*gacM*ttgA*acaQ*ttgD:2*gatA:1*tctK*gagD:2*cagT*tccG*tcgA*aacD:2*tcgL*gtgL*tttL*gctS*tctK:1*aagN:2*atgL*gaaS*gacE:1*aagE*gcaN*aaaA:1*gagK*acaM*cagK*tctQ:3+DERGD*aacD*ccaS:2*gaaL*cagR*aagR:1*aaaE:3*gagI:1*cttY:1*attA*gtgL*cagN*caaE*accA*atgF-gat*gatN*gccS:1*cagA*gagL*cttH*gccQ*agaE*gtcQ:2*gagN:1*gatS*gagN*gctG*attG*aacS*gaaT*caaP:1*gatA:1-gtcctgattgatcacctcactaag:1*acaH:1*ttgI*gttI:2*atcL:1*gctE:2*atcS*aaaQ:1*attV:2*tccA:1*tacF:2*gacE:10*tcaT:6*atcV:4*caaS*tcgE:2*accS*gacA:2*aacT:3*gacN:1*attL:2*ggtE*ccaT*aatD:2*cacY*tctA:3*agaK*tccT*attV:1*gaaI*ttcY*acaS*tctT*tctA*atcV*gccE*gatN:2*ctcS:1*gccS:2*attL*gtgT:2*gtgA:1*actD:1*agtA*gaaS*tctA*aatD*cagA:1*attV*gccN*gccF*tccA:1*tctE*accS:1*cagE*gaaA*tctT:1*gacR*ttcN*cttF*atgI*tctG:3*gatE:1*cttI:1*ggaD*actF*gagP+L*gaaD*gagD:1*accM:1*gttR:2*tccT*ggtL:1*ttgM:3*gagT*aagA:2*gaaH:1*attT*gttK*attL:2*aacR*cttM:2*agaK*tctT*gttN*ctcI-aag:1*attM*aatS:5*gctL:1*gatE*aacR:1*ttgM*aacA*tctK:2*tctD:2*acaA*tctA:1*gctS:1*catK:1*aagG:3+KFNQ*aacS*tccD*tctP*ttcL*gatK:1*tccT*atcT:1*ggtL*gatQ*atcL*aacH*aagE:1*atcA*ttaI*tctQ:2*attQ*tccA:1*accI:1*tcaA:2*cttA:2*cagR:2*attT*gagD*acaA:5*acgA*aaaQ*aatG*cagR:1*agtT*ggtS*accS:7*cacN:1*agaK:10*gctS:5*aagN:3*ggtE*actK:3*gtgT:1*agtR*tctR*aagT*aacS*accG*catL:8*gaaN:24*acgK:2*aatE*gctC:2*actV:6*tctN:2*aacK:3*gatE:3*cagK*agaK*acgF*aatG*ggtE*ggtL:1*cagE*gatK*attV:1*ttcY*tcgA*aagA:1*acgS*aacK:2*aacF:2*atgT:13*gccD:1*gctQ*tccG:6*gagQ*attM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gatE:1 +CM115473.1 miniprot mRNA 648035 651154 2623 + . ID=PF000017;Rank=1;Identity=0.5141;Positive=0.7171;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +CM115473.1 miniprot CDS 648035 651154 2623 + 0 Parent=PF000017;Rank=1;Identity=0.5141;Target=4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved 1 1046 +##PAF 4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1054 0 1047 + CM115473.1 3009164 648034 651154 1641 3195 0 AS:i:2612 ms:i:2612 np:i:763 fs:i:0 st:i:0 da:i:0 do:i:3 cg:Z:99M4I179M1I13M3I14M6D6M4I14M2I16M2D226M5I22M1D23M8D133M1I34M1D25M1I7M4I211M cs:Z::2*aagS*acaN*atcR*tccA*gacE*tctV:2*attN*tcaV*tcgN*ttgI:4*tctN*ctcT:2*actS:14*tggH:2*aagR:2*agaK*cagA:1*ttcW:1*cagG*ctgI*aaaR*ctgI*ctcQ:1*gttL:1*ggtT:1*gaaD:1*caaM*ctcV:2*acaA:12*ggcP:3*aagR*gagD:1*atgQ*cgtA:1*gctV*gatN*tggF:1*gagQ*ggcS:1*ggaA:1*tcaG*gtcN*catN+YTGP*ggtN*gatQ:1*ttcN*aagH:3*agaK:2*aagS:3*gtcR*tatF:1*ttaM*agaQ:2*aacT:3*aacY:1*cgtP*ggaA:3*acaM:4*gagD:1*gtcI:2*gttR:2*caaN*aacD:1*gacN:6*ctcM*gatN:6*gctG*ctgV:2*cttF:3*atcV:1*gctS:1*atcL*tccQ*catR*gacS:1*aagS*tctN:5*gctS:3*ttgM*gttI:7*aagR:1*attV*acgI:5*attM:1*cgcV:3*tctN:5*cctS:2*gaaQ:1*tatF*gagY:3*gccD:2*tacR*gagD:2*gctY:3*tcaT*atcL*cgcK:2*actR:1*ttgI*attV:3*agaQ:1*ccaG*gaaK*acaN*gcaP:3*atcL*tccE*aaaE*gatE*gagD*agtG*gacP*gtgG*gaaL*agtP*gaaQ:2*gccK:1+V*agaA*caaE:1*tcgR*gtcS*gctV*acaS:1*gttE:1*gaaA:1*atcL+PVA*tcgT*gaaP*cagT:1*actS*atgI*ggtP*aacA*ttcE*caaA:3*gcaE-tctcagccaactggtgtt*gatN:2*aacS*accQ*caaD+ALLA:1*aacQ*gtgQ*tttY*gaaD:3*cagE:2*gcaR*agaQ*gaaQ+QA*gccM*caaE:1*aacE*atgR*cttI*caaR:1*cagM:1*cagM*gctQ*gagQ:2*cgtQ-atttgg:2*cagM:2*gctR*gctQ:1*gctM:1*cagA:1*aggQ*ctaR:5*ttgM*cgcA:2*ttgM*cagA*aacR*cagH:1*gagQ:2*gtcM:6*ttgI:2*atgL:1*caaG:2*gagD*aacQ:3*ttaM:1*cagE:3*caaG:3*accA:2*cagA:1*attL*gccN*accQ*acaL*acaQ:3*actH:1*caaS*cttA*ggtQ*tctA:2*gagD:1*ttgI*aacE:3*gaaQ:1*ttgI:1*ttaM:1*aaaR*aatQ:2*gatE*tctT:3*ctgR:2*cagS*ttgM:1*cagE:1*catY:1*aacA:2*aacK:1*tttL:1*aagA*ttaT:7*cagK:4*cgtK*cgtA:2*cttM:1*aagR:1*ttgM*aagR*tctH:2*gttI:3*ggtD:2*agaK:6*ttgY:2*gagD*aaaR*attA:3*aaaN*gacK*agcE*gagD*attV*gatE*aagR:2*ctcR*cagE*atcL:1*gacM*ttgA*acaQ*ttgD:2*gatA:1*tctK*gagD:2*cagT*tccG*tcgA*aacD:2*tcgL*gtgL*tttL*gctS*tctK:1*aagN:2*atgL*gaaS*gacE:1*aagE*gcaN*aaaA:1*gagK*acaM*cagK*tctQ:3+DERGD*aacD*ccaS:2*gaaL*cagR*aagR:1*aaaE:3*gagI:1*cttY:1*attA*gtgL*cagN*caaE*accA*atgF-gat*gatN*gccS:1*cagA*gagL*cttH*gccQ*agaE*gtcQ:2*gagN:1*gatS*gagN*gctG*attG*aacS*gaaT*caaP:1*gatA:1-gtcctgattgatcacctcactaag:1*acaH:1*ttgI*gttI:2*atcL:1*gctE:2*atcS*aaaQ:1*attV:2*tccA:1*tacF:2*gacE:10*tcaT:6*atcV:4*caaS*tcgE:2*accS*gacA:2*aacT:3*gacN:1*attL:2*ggtE*ccaT*aatD:2*cacY*tctA:3*agaK*tccT*attV:1*gaaI*ttcY*acaS*tctT*tctA*atcV*gccE*gatN:2*ctcS:1*gccS:2*attL*gtgT:2*gtgA:1*actD:1*agtA*gaaS*tctA*aatD*cagA:1*attV*gccN*gccF*tccA:1*tctE*accS:1*cagE*gaaA*tctT:1*gacR*ttcN*cttF*atgI*tctG:3*gatE:1*cttI:1*ggaD*actF*gagP+L*gaaD*gagD:1*accM:1*gttR:2*tccT*ggtL:1*ttgM:3*gagT*aagA:2*gaaH:1*attT*gttK*attL:2*aacR*cttM:2*agaK*tctT*gttN*ctcI-aag:1*attM*aatS:5*gctL:1*gatE*aacR:1*ttgM*aacA*tctK:2*tctD:2*acaA*tctA:2+S:1*catK:1*aagG:3+KFNQ*aacS*tccD*tctP*ttcL*gatK:1*tccT*atcT:1*ggtL*gatQ*atcL*aacH*aagE:1*atcA*ttaI*tctQ:2*attQ*tccA:1*accI:1*tcaA:2*cttA:2*cagR:2*attT*gagD*acaA:5*acgA*aaaQ*aatG*cagR:1*agtT*ggtS*accS:7*cacN:1*agaK:10*gctS:5*aagN:3*ggtE*actK:3*gtgT:1*agtR*tctR*aagT*aacS*accG*catL:8*gaaN:24*acgK:2*aatE*gctC:2*actV:6*tctN:2*aacK:3*gatE:3*cagK*agaK*acgF*aatG*ggtE*ggtL:1*cagE*gatK*attV:1*ttcY*tcgA*aagA:1*acgS*aacK:2*aacF:2*atgT:13*gccD:1*gctQ*tccG:6*gagQ*attM:2*tatV*ggaA:1*agaL*gatH*ggtQ:1*agtE*gatE:1 +CM115473.1 miniprot mRNA 648035 651154 2612 + . ID=PF000018;Rank=1;Identity=0.5136;Positive=0.7164;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +CM115473.1 miniprot CDS 648035 651154 2612 + 0 Parent=PF000018;Rank=1;Identity=0.5136;Target=4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved 1 1047 +##PAF ONH67106.1 254 0 254 + CM115473.1 3009164 636429 638454 762 762 0 AS:i:1262 ms:i:1299 np:i:254 fs:i:0 st:i:0 da:i:0 do:i:0 cg:Z:69M1263N185M cs:Z::69~gt1263ag:185 +CM115473.1 miniprot mRNA 636430 638457 1299 + . ID=PF000019;Rank=1;Identity=1.0000;Positive=1.0000;Target=ONH67106.1 1 254 +CM115473.1 miniprot CDS 636430 636636 348 + 0 Parent=PF000019;Rank=1;Identity=1.0000;Target=ONH67106.1 1 69 +CM115473.1 miniprot CDS 637900 638457 951 + 0 Parent=PF000019;Rank=1;Identity=1.0000;Target=ONH67106.1 70 254 +CM115473.1 miniprot stop_codon 638455 638457 0 + 0 Parent=PF000019;Rank=1 diff --git a/results/2026-10-30_petasospora/data/all_refs.faa b/results/2026-10-30_petasospora/data/all_refs.faa new file mode 100644 index 00000000..c39615ac --- /dev/null +++ b/results/2026-10-30_petasospora/data/all_refs.faa @@ -0,0 +1,137 @@ +>MH260578_SUI1 +MSNIENLKSFDPFADTGDDETEVTNYIHIRIQQRNGRKTLTTVQGLPDEYDLKKILKVIKKEFACNGNIVKDDELGEVIQLQGDQRLKIMEFMVQTLGIKKKNIKIHGF +>MH260578_SLA2 +MFSSLVSREGETKRSRKTSSNMFSSSRHVTDISTAIKKACTPEDTASKRKHVRACIVYTWDHKSSKDFWETMKVVPLKNTDTQLFKALILIHKVLQEGHPSALVGGFKNMEWISNLDTLRTLDRRFQVLIPEYVNYILQKLRFHHNHRGFNGTFEYEEYVSLRTVSDPNEGYDAILDLTSLQDSLDSFGRDIFATVRNNNSECIISALVPIVAESYGIYKFLISMLRAMYKMSDSAEILEPLKSKFEDQHERLYDFYANCSNIRYLTTLITIPKLPFEAPNLIIQNDEDENVNLPPLTEQKTAATTLRHSPTPFEQENERQQQQAPIATQPTGMVRNAFVEQQQLYEQQQQQLEQQRQAELQQQQQQQLAQQQYWEQQQRQQAEAQQLAQQQLLLDQAQRHAQGRVAELERDILTLKGQYDNDQLMLQSYDQKVQALENELASTTQTAQEQLASKDEQLNLLQEQVEYWKNKYESLAKLYSQLRTEHLNLLTKSKKIQQKAASAQEAIEKREKLERDMKAKNIELADLIKERDRARLELERFKNGTKTDLQTLQLEKDELEQKLATLDRAQSANLTAIFNQHNKELQELQNKFKDVTLNAPSDEKLKQLEEQLQEKDLEIEMMQQTMDETIKDLVKTQNSREEKSEMQNKLNSTKVLGLIDAILKSGINRIQDAVFELDSPMQAGNLNSSPEYVLTLLEKSSGLVTDFASSFNNMLVDGDNANEALVIDSITNFTTSISDILLNTKGLTRLTKQDDFQDDLIDTAKDIAEISEVYLESLYHSNLVNKSIDDQTDIVINGNIDVQEILQTLLQLVESMKTPSSKIDLSKVDGELSDLVDKEMEHASRVIEEASVHLSSLLNQPFDVSMSSIDIEVNKSILAAASAIITAIKYLIRASIESQEEIVNNGRGSHSKTSFYKKNNKWTEGLISASKSIAYSTNTLIRIADGVLQNKHSNEELVVASREVAASTAQLVSAARVKSQFMSKTQNKLEDASKKVNLACKQLVDQVNKLIIGKHEVEDIDYSKLSIHENKTAEMEQQVEILKLENALSSARKRLGEIRKFSYKDDDSDEE +>MH260578_MATalpha1 +MARMWNKSSTLRVKKQRPSRICKTTTKATIFIAKPTPRYVIPLEVEELLGKYISRNNLDLRTLSNVKKKNKKHKTVVNGFTAFRTYYSKFGKTYEDQENLSKELANLWKQTPSIQSTWRGYSEEYRASETDLPFVEWFDTYKSLIKPEPEKRDYITQTTTMSHLTVEDVYDNLGSNSMN +>MH260578_MATalpha2 +MQHDELLEVYIHLKDVLRQLAETPIDEELSSTDLLKDMLNIKEILESGSPLQKQSCELIRMIRRISTSIYDMISQRSTLKTKYGLDLKSWDLDNSMMNTEDTNLTKNMDIHNSSSIDIDIDDEEISTALRDEGIHSSFCNTDSNSIKTKDGSSRNGKRLPKETIAVLNKWFNDNIENPYVQKEDISYLKAKTNLQASQIKNWVSNKRRKRKGSKVSPSIVEILN +>MH260578_TGL1 +MNLLIIFLNLVSAVVQLPLLIFHKLGVEEYLSYFQTPSNRPDNISAVSQENKTKISPSAKPLHEVATTHCLHQKIIKANDIVDIVHAHGYKVHEHIVQTKDGYLLSIHRILKSSGDKPQNSRVVYLHHGLLTNSELFLLGENKEKCLPFLLVDQGYDVWLGNNRGNKYSRKHLFLSSTDEKFWNYSLDEFAMFDIPNTVNYILRNTQQDSLSYIGFSQGSAQCLAALSLNHDLNKKINKFIGLSPAMIPQGLNNPIASFLVTSAPGLLYRIFGKRAILPSVVFWQKLMGPVLYEKVVDKSLVFLFNWKSGNIKTSQKKVGYPHMFSPSSVKSVAHWFQIIDSKRFQMFDEGGASGSRLVYLSGTSGKTNRVASFPTQTITTPCLFVYGKSDMLIDIERTLDNLSCDIQTIGIDGYEHMDTLWADQVEKKVFPVVLQYLKQ +>MH260578_SEC3 +MSQRSHGETGGGQRLFVPTHKPMNSMDLARQYEMDMHNITKYLFKEVDAQGYKLESFVSYVRIIEDSRSPSSRPPANSPLSNKKNRFLILSAKASGRMRLHKAKESSSGVIQIGRSWDFDELTTLELDDGSPTGFVCEMGKRYYWEVHTPKERRVWCTTLLQNFINYTKGNIPRLVNCSIQYFHLEALYDSYRGQGSSMYTGSGNIPASPLSNQNAMSSPVKTSVKSSLRSPLRSPIKSPIKSPTKSHTNIQRSPIKSSSPEVSSLKQRLATPPLSNSPYSPLGNTRTNLGTAVAGNANNAFGLGVGISPVSKSSEVSSDHQDAEREAKKIAEINKRRQQEIEKRKREELERRRREQEEFEKNEREQNELRIAEEIEERKQKEEQKRLQEMEKEREVQRIKQEELEIERQKQERIDEEKRLKEMEIAKKKAQKQHHQQELLRNEIKPPGFAGIAKRGPPSEALSNAPSQMSFEYGDESQYHHQNVSTESANTIGVDSFIEGYGSSGDAEEETAPLNIGIAQVKQNKDNKMVPSNLSKPVITETIEESDSDRSLKIATDSDIEKLIKNVDGSPMDKKKVIDTEQLHENRIRAFSRVKENATNKLDLLEVLEELGYDPLFDDSESLEKKILKKLDEIQYSKIKTLTDVTSATNALKETISTAFKSCDVIDPVLSLFSVELSTFKDDVHYIEEQGQGLQVEATNEKLLMNEVNDLIHSVEISDSKLQKLLTSKIVLSENNSEFEDILLELYNALKKMNDASSDDPMSANYQLSQMKALQEKKNVFEGSKNKFVSNFTRISEKLFKSVSMSLTTRLQQVTPETFETNFLQTSFMEKSSLLITISGFIAFVKSVSNDGYDSIVNSFVVAFEPFFENMATILLQHLTSVASSKLEEPFSFTLNPSQLIDQEYINSRAKKDSAKTLFNRNNKLSTEATTISSNVETFFSQLVNIMSIEQELIRVLFGFSSSSDCLFENLVKVKLSQRCQKFRNTSNFLKAPIESDRRVGDEIYELMKGIFDPVFGAVLKVIFSLSKNHMLEIPAILSILKTYSQSLAPTCHEYAYGNFTKFEARINTIWTKEIDQQIQNIVHTKVHCKVANFVKAYPVFYRRVNNFIDSLNLVNMTSFGPDEGWYGNYYMVWAIIKAALNKGVEQLQIEIPVFEGIENDEIDIETLTQKHLALLLNYKWLVEETRSLNGFPKELNKSIDDLRDKELREFTGSFIRQFRIGGVIRLVEGMESVLSEHSNPANMNSYSIETIKELMSSFKGDNFKQEISSIAEHLKETLKGRCYNESDPTEDSEYSVAIGKQIEKELYNNCMYTISQHFISIFTRLSNIVEKHYINFEVPVDKYIINFNFKKYYSV +>460519_cbs-7435_MATkp_MATa_MATa2 +MTVTTFLSSSFDSVAFAMTCVSDKSYKTTAGKGLSGKKFNLSTHRPRNKFIISKSIFSKVIQRYYPDLSFEENSKIASYLWKHRFGCFDKYYEYLQIEESKFTSLVTSKIDQLAFKAVPTEKTTSQCTFRFSSSPKRRRTVNLNILKPLCKRVYSQQFHSPLTGITTQDIFVAEENNV +>460519_cbs-7435_MATkp_MATa_MATa1 +MSGNTFREITAACLLSMILSTDDYTKPATSCHMHQLTAPDLSDYSLLDALFVTCDRALEYQALTYRLQASMEDAMKIIESARYQTKDLPTLDNIFKTFYKTLLDTIESIEATGTSTLLSDFTHQISFFNIDPSVISLSDETKECAYSFFSDSLHHHPVKRTRKTISKEVKKILEEAFKAKAAPNTKERLLIAQRCALTPLQVRMWFTNKRTRNKIKQRLQAQSHP +>460523_cbs-4732_MATog_MATa-MATalpha_MATalpha2 +MDDMLTKIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKLCKSSMEGFTTERSLKYTRDCPQYAAVTTSQPRYRIEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTRCSPNDVV +>460523_cbs-4732_MATog_MATa-MATalpha_MATalpha1 +MMFHVLCFFGNRTSRVANLCLQLKFPPHNDSPKMALDELNLSFLTHLPQIPTTSPLLKRLLTEYGAIDNGKDSLNKVAPKKSSRHRVQKEKKRLNGFMAFRSFYSRNIANYNSQKALSQDLADAWNREKHQQIWSLYAIQYNHSKSKEPFSRWLEMKLSNKLGKFNSDDPTTRREINVMSHIVVEDVFQECSSQY +>460523_cbs-4732_MATog_MATa-MATalpha_MATa1 +MQFTILNEPSLDSQRREGDLASENYVFGDIRKEGVRILEDSLRSERNALLAIGAGGAADRVKQHYRKLLDDWEAAIMEIRSNDKYESLCCGFAIEKAEVETEMRNTEELDVYKECLVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>5476_sc5314_MTL_A_MTLA2 +MPYTFNFPKSQSSFKYSIKSLTNSRSFPMISIYVTPQSTPKFINNQSIDKMTVLSSFRSRNSFIIARSILSKLLRKNKADFKKVSKSVSLLWGSVDNSFKNYFEYLSILESQWHDKKSSNLNPISSTRTTSELNYIELCSSIYNQSRKHPRSVQGNKAKFRLYKNVSKKSNRKPRRLKNGFKVSRSTINCSYGIYTEDVFQ +>5476_sc5314_MTL_A_MTLA1 +MNSEIESSLTLLKSVEKLVQATSVYKNEDNEEIFLQLKRERQENSNSHEETETFFHESLDRLMKLSSIAGSKVQYELSLNNLYNLLLRTRKQEEEEAFIFPTVSTEEFALEQITFEVSDQMDDDKDSEDDILIKGEEIKKSKKKRQRLDNSTKEFLEKVFEKNKQPNRRERELIAEKHGVSLSQIRVWFTNKRMRKKEPKSKAKSSSNST +>1005962_dl-1_MATog_MATa-MATalpha|gene_index=0|name=sla2|role=flanking_conserved +MLSFGSHSNLDIQASLKKACTADAAAPKRKHVRACIVYTWDHKSSREFWHCLKLLPIQSNDTQIFKTLIVIHKVLQEGHPTCLIGGYKNISWLESLSRFSNNGTAAGYTRLIKEYVFYLEQKLKFHHDHRGFNGMFEYEEYVSLRTVSDPNEGFESIMDLLSLQDSLDNLQRVIFSSIRHTSESECVISSLVPIIAESYGIYKFLISMLKALYKSSESDEVIAPLKDRFDVQHRRLFEFYADCSSIKYLTTLVTIPRLPSSPPILQDEEDPQGFPLVRERSSVSNSEESNIPRSSNIQSQPTGAMVDSFVRQQQELEEQQRQAEERRQAELLEQQMLQRDQARFWEEQQRQQAVSQELAQSQLLADQMQRQAQGKLAQLEQDLLTLGGQHERDQLMLEQYDQRVKVLENELRIVTQTAEEQLAVKTSQCSHLEEQVQYWKNKYESLAKLYSQLRQEHLNLLGKFKIVQQKAASAQESIDKREKLEKDLKAKNIELADLIKERDRARLDLARIKGNDSEQVQMLRTQLRDMETKLKSLEETHSQNLASLFSQHSKEIEEIKASASELKEGGLNEEAQQEVQMLQQAMDDTMQELAEQHQAIEKEAETRVLRLIDTLLKVSSDRLREFVFNLESPVYSNTDIPTAEYLGTMLERVSHLSTQFSTTMTDYIADNMKGESESGLIKAIIELTDSIGNLFSNVKAVLRDRKDDVQEILVEGSKDLAHMVELFFESMLSNHLLKLEIHERTDRVITGNINVQEVIQSLMQVVEVFRDPGRKTPVSSDELGYFLDKELANTQAAVEKAAGLLESMSTRHKLEVQEDGSLEVNEAIISCASAIINAVKLLLEACIDAQEEIVSRGRGSMSRALFYKKNNRWTEGLISASKQVAYATGILIRMADGVLAGTNSSEELIVASNEVASATAQLVSSSRVKSDLMSQSHLNLEEASRKVTSSCKMLVTKVRSLLLSDESNKTVDYSQLTAHENRTAELEQQVEILKLESALSHARKRLGEIRKFSYRDEES +>1005962_dl-1_MATog_MATa-MATalpha|gene_index=1|name=MATa1|role=core_MAT +MQFIILNEPSLDSQRREGNLASENCVFARDIRKEGVKVLEDSLRSERNALLAIGAGGAVDRVERHYRRLLDGWEAAIMEIRSNDKYESLWRGFAIEKAEVETEMRKVEKQWPGELDVYKECFVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>1005962_dl-1_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT +MFLAFFVIARVGLQNLCLQRKFPPHGDSAKMALDGLNLSFLTHLPQIPTTSPLLKRLLSEYGAIDNGNDSSNKVAPKKSRRHRVQKQKKRLNGFMAFRSFYSRNIANYNSQKALSQDLADAWNREKHQQIWSLYAIQYNHSKSKEPFSIWLEMKLNKKLRKNNSDDPTTRREINIMSDIVVEDVFQECSS +>1005962_dl-1_MATog_MATa-MATalpha|gene_index=3|name=MATalpha2|role=core_MAT +MSSYEEQCSAPDLLLLLDRLIDSITESDLSLEDELYWDSHVHSLMAVITHTVSSPSGTPMDDMLGQIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKFCKSSMESFTTERTIEYTRDCPPYAAVTTSQPGYKTEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTDCSPNDVV +>1005962_dl-1_MATog_MATa-MATalpha|gene_index=4|name=dic1|role=flanking_conserved +MNSDKSTKSIAPSANFPFWYGGAASMFACLFTHPLDLAKVRLQTAKVPGDSLVSLAFKIIKTEGVLAAYAGLTASLLRQATYSTARFGVYEKLKEIMTDPTRGQASTFQLLAASMIAGAVGGVVGNPADVVNIRMQNDNSLPESQRRHYKHALDGLLKITREENLTALFRGLGPNLARGILMTASQVVSYDVAKKLLVENLSMDPKTKATHFSASLIAGLVATTVCSPADVLKTRIMNSSGTGQSSFGILKDAISREGLGFMFRGWTPAFIRLGPHTILTFIALEELRRLKIGL +>460519_cbs-7435_MATkp_MATa|gene_index=0|name=dic1|role=flanking_conserved +MTQINRESNDDSTMQYERIPPAKGVFPFWYGGAASMFACLFTHPLDLAKVRLQTASQPGQNLLTIAIRIISSEGILGAYSGLSASLLRQATYSTTRFGIYEALKDYLTKANNNQDLSTAILLPCSMLAGGIGGLVGNPSDVVNIRMQNDSSLPKPARRNYRNALHGLARMAKEEGLGSWFRGLLPNLTRGVLMTASQVVSYDVAKKFLVKKLSFDETTRATHFSASLMAGLVATTVCSPADVVKTRIMNSAEKHQSIITVLTTAMRKEGPGFLFRGWFPSFIRLGPHTILTFVALEQLRTWRIAM +>460519_cbs-7435_MATkp_MATa|gene_index=1|name=MATa2|role=core_MAT +MTVTTFLSSSFDSVAFAMTCVSDKSYKTTAGKGLSGKKFNLSTHRPRNKFIISKSIFSKVIQRYYPDLSFEENSKIASYLWKHRFGCFDKYYEYLQIEESKFTSLVTSKIDQLAFKAVPTEKTTSQCTFRFSSSPKRRRTVNLNILKPLCKRVYSQQFHSPLTGITTQDIFVAEENNV +>460519_cbs-7435_MATkp_MATa|gene_index=2|name=MATa1|role=core_MAT +MSGNTFREITAACLLSMILSTDDYTKPATSCHMHQLTAPDLSDYSLLDALFVTCDRALEYQALTYRLQASMEDAMKIIESARYQTKDLPTLDNIFKTFYKTLLDTIESIEATGTSTLLSDFTHQISFFNIDPSVISLSDETKECAYSFFSDSLHHHPVKRTRKTISKEVKKILEEAFKAKAAPNTKERLLIAQRCALTPLQVRMWFTNKRTRNKIKQRLQAQSHP +>460519_cbs-7435_MATkp_MATa|gene_index=3|name=sla2|role=flanking_conserved +MSRQEADLKVSIKKACSTEEAAPKRKHVRACIVFTWDHRSSKAFYNGLRLLPIQNDEIPLFKSLITIHKVLQEGHPSAIKEGIKNRDWIQSLGHVFPGDGMKRYGRLIREYDRYLIRKIDFHNSHKGFNGTFEYEEYVSLKTVSDPNEGYEAIMDLMVLQDSINDLQRLLFASIDSSSHSELKISALVPLIAESYGIFKFITSMLRGMCHSLGHDDEALQPLIERFISQYSDLYDFYAECSYVKYLTSLVNIPKLPFEAPDLFNWDETDELKPRILAEEPSLQVSSKAPSEEFGTPTEVIHSQPTAGTDLWAQSNALKQQYEQEQQLLEQERQQQLLEQQHQQQQQLEYWEKQKELQEQQQLYAQQQLESDQLQRQVQGRVGELERDLLILRGQFDRDQLMLRQYDERVKALETELSSTTQNAQQQLGAKEELLGNIQEQAAQWKSKYDSLAKLYSQLREEHLNLLSKFKTAQLKASSAREAIDKREKIERDLKAKNIELADLIRERDRARLDLERSKSDKNSETERLEARLREQETKLADIEKLHSSELKSFLSQHNKEIEELQNQLQASSLSESANERLKALEAQLQEKDDELAIAHQTMEATIKELTGQQSIQNSAIDEQIDAILLEHIDKLTEVIDAILTSGVQRIQDGLFELDSPMQAGNQNSSPEYLSSIIEKSSNLATEFTSSFNGFIADGPNGDHVSVIQTITEFTTSIVDLLLNCKGVVRLASSDQEAEALLNSARDVAEESESFIVSLYSSSLKGLSDQEKTDKAIEGNVDIQQELQTLSSLVEKLISSQVNINVNDNVGELVDREMAHAAETIAKASDHLTDLLTNAKQDISTIDMKINNSILSAAIAVTKAIILLIKTATDCQNEIVNHGRGSSSRTSFYKKHNKWTEGLISASKSVAYSTNSLIQIADGVLSSTSSPEQLIVACNDVAASTAQLVAASRVKASFMSKTQDKLELASKSVTSACRSLVRQVQEILSQKDKEELEIDYSKLSAHENKTVQMEQQVEILKLETALQNARRRLGEIRRFGYHADEE +>460519_cbs-7435_MATkp_MATalpha|gene_index=0|name=sla2|role=flanking_conserved +MSRQEADLKVSIKKACSTEEAAPKRKHVRACIVFTWDHRSSKAFYNGLRLLPIQNDEIPLFKSLITIHKVLQEGHPSAIKEGIKNRDWIQSLGHVFPGDGMKRYGRLIREYDRYLIRKIDFHNSHKGFNGTFEYEEYVSLKTVSDPNEGYEAIMDLMVLQDSINDLQRLLFASIDSSSHSELKISALVPLIAESYGIFKFITSMLRGMCHSLGHDDEALQPLIERFISQYSDLYDFYAECSYVKYLTSLVNIPKLPFEAPDLFNWDETDELKPRILAEEPSLQVSSKAPSEEFGTPTEVIHSQPTAGTDLWAQSNALKQQYEQEQQLLEQERQQQLLEQQHQQQQQLEYWEKQKELQEQQQLYAQQQLESDQLQRQVQGRVGELERDLLILRGQFDRDQLMLRQYDERVKALETELSSTTQNAQQQLGAKEELLGNIQEQAAQWKSKYDSLAKLYSQLREEHLNLLSKFKTAQLKASSAREAIDKREKIERDLKAKNIELADLIRERDRARLDLERSKSDKNSETERLEARLREQETKLADIEKLHSSELKSFLSQHNKEIEELQNQLQASSLSESANERLKALEAQLQEKDDELAIAHQTMEATIKELTGQQSIQNSAIDEQIDAILLEHIDKLTEVIDAILTSGVQRIQDGLFELDSPMQAGNQNSSPEYLSSIIEKSSNLATEFTSSFNGFIADGPNGDHVSVIQTITEFTTSIVDLLLNCKGVVRLASSDQEAEALLNSARDVAEESESFIVSLYSSSLKGLSDQEKTDKAIEGNVDIQQELQTLSSLVEKLISSQVNINVNDNVGELVDREMAHAAETIAKASDHLTDLLTNAKQDISTIDMKINNSILSAAIAVTKAIILLIKTATDCQNEIVNHGRGSSSRTSFYKKHNKWTEGLISASKSVAYSTNSLIQIADGVLSSTSSPEQLIVACNDVAASTAQLVAASRVKASFMSKTQDKLELASKSVTSACRSLVRQVQEILSQKDKEELEIDYSKLSAHENKTVQMEQQVEILKLETALQNARRRLGEIRRFGYHADE +>460519_cbs-7435_MATkp_MATalpha|gene_index=1|name=MATalpha1|role=core_MAT +MLPPQPKSFKGIQKPFKCSQYAHSTLDNGNMLQHTKHPELVTYQLETPRTRSLQQIESHLLQETTLGIKRTGENQYSEKRTKSSRSYMSKRINAFIAFRSYYSRQVNDYNKQISISRLLSVAWKNENKELWRSYALLYNSSNSNIPFVDWLQTKGPKNSKRTSTVTKSFISWNELLSTRNKIEDVFLDLQ +>460519_cbs-7435_MATkp_MATalpha|gene_index=2|name=MATalpha2|role=core_MAT +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRAIALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLSKTQIRNWISNKRRKERSSTVSTELLQALNKSPS +>460519_cbs-7435_MATkp_MATalpha|gene_index=3|name=dic1|role=flanking_conserved +MTQINRESNDDSTMQYERIPPAKGVFPFWYGGAASMFACLFTHPLDLAKVRLQTASQPGQNLLTIAIRIISSEGILGAYSGLSASLLRQATYSTTRFGIYEALKDYLTKANNNQDLSTAILLPCSMLAGGIGGLVGNPSDVVNIRMQNDSSLPKPARRNYRNALHGLARMAKEEGLGSWFRGLLPNLTRGVLMTASQVVSYDVAKKFLVKKLSFDETTRATHFSASLMAGLVATTVCSPADVVKTRIMNSAEKHQSIITVLTTAMRKEGPGFLFRGWFPSFIRLGPHTILTFVALEQLRTWRIAM +>460519_gs115_MATkp_MATalpha|gene_index=0|name=dic1|role=flanking_conserved +MTQINRESNDDSTMQYERIPPAKGVFPFWYGGAASMFACLFTHPLDLAKVRLQTASQPGQNLLTIAIRIISSEGILGAYSGLSASLLRQATYSTTRFGIYEALKDYLTKANNNQDLSTAILLPCSMLAGGIGGLVGNPSDVVNIRMQNDSSLPKPARRNYRNALHGLARMAKEEGLGSWFRGLLPNLTRGVLMTASQVVSYDVAKKFLVKKLSFDETTRATHFSASLMAGLVATTVCSPADVVKTRIMNSAEKHQSIITVLTTAMRKEGPGFLFRGWFPSFIRLGPHTILTFVALEQLRTWRIAM +>460519_gs115_MATkp_MATalpha|gene_index=1|name=MATalpha2|role=core_MAT +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRAIALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLSKTQIRNWISNKRRKERSSTVSTELLQALNKSPS +>460519_gs115_MATkp_MATalpha|gene_index=3|name=sla2|role=flanking_conserved +MSRQEADLKVSIKKACSTEEAAPKRKHVRACIVFTWDHRSSKAFYNGLRLLPIQNDEIPLFKSLITIHKVLQEGHPSAIKEGIKNRDWIQSLGHVFPGDGMKRYGRLIREYDRYLIRKIDFHNSHKGFNGTFEYEEYVSLKTVSDPNEGYEAIMDLMVLQDSINDLQRLLFASIDSSSHSELKISALVPLIAESYGIFKFITSMLRGMCHSLGHDDEALQPLIERFISQYSDLYDFYAECSYVKYLTSLVNIPKLPFEAPDLFNWDETDELKPRILAEEPSLQVSSKAPSEEFGTPTEVIHSQPTAGTDLWAQSNALKQQYEQEQQLLEQERQQQLLEQQHQQQQQLEYWEKQKELQEQQQLYAQQQLESDQLQRQVQGRVGELERDLLILRGQFDRDQLMLRQYDERVKALETELSSTTQNAQQQLGAKEELLGNIQEQAAQWKSKYDSLAKLYSQLREEHLNLLSKFKTAQLKASSAREAIDKREKIERDLKAKNIELADLIRERDRARLDLERSKSDKNSETERLEARLREQETKLADIEKLHSSELKSFLSQHNKEIEELQNQLQASSLSESANERLKALEAQLQEKDDELAIAHQTMEATIKELTGQQSIQNSAIDEQIDAILLEHIDKLTEVIDAILTSGVQRIQDGLFELDSPMQAGNQNSSPEYLSSIIEKSSNLATEFTSSFNGFIADGPNGDHVSVIQTITEFTTSIVDLLLNCKGVVRLASSDQEAEALLNSARDVAEESESFIVSLYSSSLKGLSDQEKTDKAIEGNVDIQQELQTLSSLVEKLISSQVNINVNDNVGELVDREMAHAAETIAKASDHLTDLLTNAKQDISTIDMKINNSILSAAIAVTKAIILLIKTATDCQNEIVNHGRGSSSRTSFYKKHNKWTEGLISASKSVAYSTNSLIQIADGVLSSTSSPEQLIVACNDVAASTAQLVAASRVKASFMSKTQDKLELASKSVTSACRSLVRQVQEILSQKDKEELEIDYSKLSAHENKTVQMEQQVEILKLETALQNARRRLGEIRRFGYHADE +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=0|name=dic1|role=flanking_conserved +MEVCITTQSIDNGVGAASMFACLFTHPLDLAKVRLQTAKVPGDSLVSLAYKIVKTEGVLAAYAGLSASLLRQATYSTARFGVYEKLKGIMTDPTKGQASTFQLLAASMIAGAVGGVVGNPADVVNIRMQNDNSLPESQRRHYKHALDGLLKITREENITALFRGLGPNLARGILMTASQVVSYDVAKKLLVENLSMDPKTKATHFSASLIAGLVATTVCSPADVLKTRIMNSSGTGQSSFGILKDAISREGLGFMFRGWTPAFIRLGPHTILTFIALEELRRLKIGL +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=1|name=MATalpha2|role=core_MAT +MDDMLTKIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKLCKSSMEGFTTERSLKYTRDCPQYAAVTTSQPRYRIEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTRCSPNDVV +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=2|name=MATalpha1|role=core_MAT +MMFHVLCFFGNRTSRVANLCLQLKFPPHNDSPKMALDELNLSFLTHLPQIPTTSPLLKRLLTEYGAIDNGKDSLNKVAPKKSSRHRVQKEKKRLNGFMAFRSFYSRNIANYNSQKALSQDLADAWNREKHQQIWSLYAIQYNHSKSKEPFSRWLEMKLSNKLGKFNSDDPTTRREINVMSHIVVEDVFQECSSQY +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=3|name=MATa1|role=core_MAT +MQFTILNEPSLDSQRREGDLASENYVFGDIRKEGVRILEDSLRSERNALLAIGAGGAADRVKQHYRKLLDDWEAAIMEIRSNDKYESLCCGFAIEKAEVETEMRNTEELDVYKECLVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>460523_cbs-4732_MATog_MATa-MATalpha|gene_index=4|name=sla2|role=flanking_conserved +QMLQQAMDDTMQELAEQHQAIEKEAETRVLRLIDTLLKVSTDRLREFVFNLESPVYSNTDIPTAEYLGTMLEKVSHLSTQFSTTMTDYIADNMRGDSESGLIKAITEFTDSIGNLFSNVKAVLRDRKDDVQEILVEGAKDLAHMVELFFESMLSDHLLKLEIHERTDRVITGNINVQEVIQSLMQVVEVFRDPGRKSPVSSDELGYFLDKELANTQAAVEKAADLLESMSTRHKLEVQEGGSLEVNEAIISCASAIINAVKLLLEACIEAQEEIVSRGKGSLSRALFYKKNNRWTEGLISASKQVAYATGILIRMADGVLAGTNSSEELIVASNEVASATAQLVSSSRVKSDLMSQSHLNLEEASRKVTSSCKMLVTKVRSLLLTDESNKTVDYSQLTAHENRTAELEQQVEILKLESALSQARKRLGEIRKFSYRDEES +>28985_nrrl-y-1140_MATsc_HMLalpha|gene_index=0|name=MATALPHA1|role=core_MAT +MKSNAPTFKVAVSKRSCSSVRKTSKKIRSGMIRKPSVSSRYRKHEGVNLYMSKVTPTSIPAPPQVLVAYIKEKVKTLNKSEVLMSLGNSNQLSSRDVKTKTKKKQINDFIAFRSYYSRLLNGILTQTELSTIISKHWTVDKQTRKNWELIAQEYNCDASGKHFFNWLEVNYGIDKQWLYEIVQYEECLTPTTKKPYVENIYNSGFKQRIDSPNQKDITFSQSDNSADWLVDPIFFDTEFLATSFDRTNLIISDCYSLMNSV +>28985_nrrl-y-1140_MATsc_HMLalpha|gene_index=1|name=MATALPHA2|role=core_MAT +MSRIPIHSLLNPSESCKSISNVPSNYRDLSTFNKERAKVITTFQEMFYSMLENNDDYNKIESLIRNFQPKLTWSHKCESLTFKQKAYLTAIIQKSIKSLLVLLKEKGKMREIEFSRKEVRKINKYRQSSKNFESVNIKILTQDLMHSNNNEFKKGKRFPKSHIQLLENWYSMNRRNPYLAENDLAYISKNTTLTKTQIKNWLANRRRKDKITEVSSDIRNILN +>28985_nrrl-y-1140_MATsc_MATa|gene_index=0|name=sla2|role=flanking_conserved +MSREEQTLEKSIKKALSGGDVAPKRKHVRACIVYTWDHKSSKTFFNLLKLQPYATDELQLFKALVLLHKVLQEGHPSALVEGIRNIDWIESLSRIFSVDSDDGSKYGSSRMRYIGDEYAKAIGEYVFFLVEKLQFHKSHKGFNGIFEYEEYVSLVSVSDPNEGYETVLDLMNLEDVADNLQKLIFAGISNRKSGFGSFSSGLDCRGAVLIPLVSETYGIFKFVTSMMKALYKQMGDYDALSGLFDRYTEQCARLNEFYFDCNTVQPLKNLITIPTLPSDPMSRIIDDEDDAEVNDVVFEKRSKSSVSSLPSRTPTRENLRDPSPVHEAPPVARPAEVRSLSQPAAPVVPIATGVVMPVVTGYSQPPLQVVPTGAEFWSIPSTSAPTRSVVASHDDLLIEQQKQQQIQQQQYALQLEHQKQLQLQQEQQALLQQRIQQQEMEKEALIQQKTASLQNDIMSFKNQYERDQVMLEQYDKRVQKLESELESVKEFKEKYQSLAKLYSQLRQEHLNILKKMKKTQDDLAEAESEVKKLKSSALSNVGIRNNNKGESEREEAKDRSGMILKSLVESSIKSLQDPIASLDVSDLATDLSNAFIDGDINTIVNILPEFVSLLLRCPQEMQTIAEDFLRCLSSAKQTDSVINANIAFQEKLKRLGEIRQYSYRYGDSDEV +>28985_nrrl-y-1140_MATsc_MATa|gene_index=1|name=MATA1|role=core_MAT +MCDNDMADIQSKLSSFCEEIRALALKEGYNLEGDKSPSSKPYFMSWPKEIDVNHPNFAFFTKLQFQYDKSLETILNSCYLQELQLDPTRIIETLQQHFNNSILRYADIENISDVKDESPMSFDTEHECTDTSEDISDKSEISSTNSDNPIQNCYPTYKRSFIKHESRGILEKIFKVKQCPNTSERLYIAQKLDLTPSQVRIWFTNKRMRAKKHTTGKGTKRKSKKYPS +>28985_nrrl-y-1140_MATsc_MATa|gene_index=2|name=MATA2|role=core_MAT +MANSTLRRTTFFKLTTTEDEDTIPKLLQPNNNSVAFPNLKRTGKAFTNDTIKENTKKYTRPRNQFVLMRTLFNRRVNNHILQYYNKSKLEKKMFTLTSKITSELWNESSPDLKSYFSLLATLEENWHKYTHYCSWDRNSAQTLSMEPIELSQVRPRLISSLTVGAGSSVSTYTLRELLLVRKIKSRKRKTTTLTQDSSPTTKFKYKFKKQPKTKMNSNLSKLRFKSKQPPTPPEENSNVFKKRYTSENRIIEDLFLM +>381046_cbs-6340_MATsc_HMLalpha|gene_index=0|name=MATALPHA1|role=core_MAT +MNSTKPSFKVLIKKKGKTLNRRLKNSSKSYRENGANLYMSYSKPQAIPKPPNTLINLVRSKKTSQSSRKCLKNDYILKELDVKKLSIYSNNTVLKKKINPFIGFRSYYAKFAKGRVRQQELSKILSEYWTKNSKIHSVWEFFTQHYNREVTSMCFTLWLEENYQQSYEESELDSECMAKRSQPYIEDLYSGTGEFLTKLTSRELLDISGNHSGFQGSLNSLPFFEEHNRTTDWMLSSILQCDQVPYYPN +>381046_cbs-6340_MATsc_HMLalpha|gene_index=1|name=MATALPHA2|role=core_MAT +MSHISVSSLLNLPVFQASHQEKATSPITSQSCLVASGKQRKFNATAKKRLERWFCSNIQYPYPNKIQILSLSKNTNLSIKQIQNWMSNRRRKSRKESIDSLLEPILNS +>4932_s288c_MATsc_HMRa|gene_index=0|name=MATA2|role=core_MAT +MRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>4932_s288c_MATsc_HMRa|gene_index=1|name=MATA1|role=core_MAT +MDDICSMAENINRTLFNILGTEIDEINLNTNNLYNFIMESNLTKVEQHTLHKNISNNRLEIYHHIKKEKSPKGKSSISPQARAFLEQVFRRKQSLNSKEKEEVAKKCGITPLQVRVWFINKRMRSK +>4932_s288c_MATsc_MATalpha|gene_index=0|name=MATALPHA2|role=core_MAT +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>4932_s288c_MATsc_MATalpha|gene_index=1|name=MATALPHA1|role=core_MAT +MFTSKPAFKIKNKASKSYRNTAVSKKLKEKRLAEHVRPSCFNIIRPLKKDIQIPVPSSRFLNKIQIHRIASGSQNTQFRQFNKTSIKSSKKYLNSFMAFRAYYSQFGSGVKQNVLSSLLAEEWHADKMQHGIWDYFAQQYNFINPGFGFVEWLTNNYAEVRGDGYWEDVFVHLAL +>4956_da2_MATsc_MATalpha|gene_index=0|name=cha1|role=flanking_variable +RNATPSLEHENIIYPKRAQVAS +>4956_da2_MATsc_MATalpha|gene_index=1|name=alpha2|role=core_MAT +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKEDQKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRSNKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVSELVKESKQGTEELNE +>4956_da2_MATsc_MATalpha|gene_index=2|name=alpha1|role=core_MAT +MNTKSFRGFKSNQPLFKVKVEKKGRSKRPTLQDTTQTSKCIAGKCIEDQQVNVFMTVSKLIKIPSPPEVLLRKIEEEKHKLKKDDFNMDNILLWDPYVYWGEGYFFSIADSSLNNLSTKGSRKGCSEKPLNSFMAFRAYNSQFGNGLKQNILSSLLAAAWHSHPEQQKIWDTFAQQFNFVKPKCGFVEWVDQRYERESCI +>4956_da2_MATsc_MATalpha|gene_index=3|name=sla2|role=flanking_conserved +SSNSNSGMSYGNVVNEEPRPVISGDVRLAIYLSRQCISNYNQPVSVLSISQLCNEVASQFLNSTPHIPTIIQLTHQITTLLQHNPSPLLAQASQLYFQALLSPSQQDIINANTNLQQLLTN +>4959_cbs767_MTL_A|gene_index=2|name=MTLalpha1|role=core_MAT +MTSEVAKVIPKRFTSRFTVSKTGKRSNSNIKNEAIDIGRQRKVKSMQVVFFTELPSLPTPSIDLQEILIRFNQATRNCAGDSSWGSQLFDMKVPTRKSRIRCKKTRCLNGFMAFRSFYSRSIYNVEHQRELSTLLGKLWKNEPNKAIWNRYAIEYNSQAINQDFVDWLFKALGLEVETFPIPYTSTVRNESWTFSSRNNENAVEDIYYIGTV +>4959_cbs767_MTL_A|gene_index=0|name=MTLA1|role=core_MAT +MEGYTKSHDVVVEVPENENIKAYTTRVESGHISEPSSSNGSNLTLNVKNEYENISSDGKVIDRYQLLKEKGYSHGFLLEEASISVTCETPDPHLQQQRKVSRFGREVHENTNVFPNKKGSYKCDSEASNRFLENVFKTNSYPTSKERHIIAKKCGLSPTQVGAWFTNRRNVSE +>4959_cbs767_MTL_A|gene_index=1|name=MTLA2|role=core_MAT +MSLSPDKIFTFCFKLPRRIDIPVESNQPTTKKNFKIKELTTTNSYPSISINIKQYVSFTSEKYIEASRKPTAKDKKTAITRGSFRTRNKFIIARNLLCRLVKHEKALSSTEISKVVSLVWKESNKSLHDYFEYLSLLETYWYDKLMSSYGNSTLQATATAAVSIRLSSVPYENLCASCYNKHKQKKTLNYDYSKSAKFRVHKDFKEKKKTFLTIKDHSKKLEKLSDAFRINQCTITTNFGIVTEDMFFNSL +>1173061_cbs615-84_MATdc_B|gene_index=0|name=MATB|role=core_MAT +MPEFKRFRFRHHKISCPSTTKNESKFLFCPQFIDFSAPKNGSEECRHTETMLAIPPPSSELLKALDELRTLQCSVQKKGIIRSRRRKPNTNMAKKVSRINGFTAFKSYYSQNITSVCQGTISSKLSKVWQIEKNQDVWDNYARHYRRYQRKEPEHFCVWLLRATQNNSIDKQKTHSFTKLNDGDENLDIEALCFNLNEDSFTKDLITTQSSGDLNPYSNYGFDTLDFITDSSKVSSDNIESSNMNDYMDGLSPESCESISPFDIIDSTFSEPTSNHYDYMFIDSPSDSLLEDFFSDKNVVSDKNCKQPFVPLELNKDQFLPMPELWVSLNSEYSLSKLS +>1173061_clib918_MATdc_A|gene_index=0|name=sla2|role=flanking_conserved +MATLSKAEAELTVHIKKATNPEETAPKRKHVRQCIIYTWDNHSGRAFWNGMKIQPLQNDEVQVFKALITIHKVLQEGHPSVLREAQQNRSWLESLARGAHAGRHTYAKLIYEYVGLLLKKLNFHKNHPDFNGTFEYEEYISLRNIDDPNEGYQAIVDLMDLQDAIDNFQRLIIASLKHGRPNECRISALIPLVAESYGIYRFTTTMLRAMHATTNDDDALEPLRSRYYSQHQRLLEFYYDCSSLKYLTSVITIPTLSQDPPNLYGENPEEGEAPALPKRPISEVASLATGATTAPSTTDLTPEPVVDFWSTPQGQQQLQQQQLQQQQYEEEQRRLAAQREAELQQQQMLHLQQQQQFEEQQRMQAEQQRLAQEALLRDQYQRQADGRAAELERDILNLRGQYERDQLMLEQYDRRVKGLEGELQQVIANTNLQSQSKDDMIRSLQEQVNSLKSKYDVLAKLYSQLRQEHLDKLKELKLLKVKAASAQEAIEKREKLERDIKAKNIELADLIRERDRARYDLDKSRGNQKDVIEKLERQLRLTEDKLADAERSKGADLSLLISKHNRELADLEEALKAKQSVLDNYANRSFDDSDLKLKLAEKDDELEILQETVDSMEAALKEMSLNHNEADQALEEQIDEVLKEHLSKLNSIIDSVLESSAERVQESLFEFESPMQAGNQNSTPEYLLSIVEKASTSATDFATSFNNFIIDGPNGDYSGIISTATILSSVIPDVLNNAKGLTRLARDDTVADNIISYARLAAVAVEDFFSGVQSNKLAQLSDEDKTDKVINFNIDVQTHLQSLSQVSEKLTPRAGTHLNGSGDLGDLVDREMARAAEAIESANAKLNELLSRPKDPKFSVFDLKIHEAILAAAIAVTTAIAALIKAASDCQQEIVAQGKGASSRTAFYKKHNRWTEGLISAAKSVAASTNVLIETADGVLSGNHTPEQLIVASNEVASSTAQLVAASRVKASYMSKTQDRLEGASKAVTSACRSLVNQVQDILSKKQQKEENDVDYSKLTPHVLKTTEMEQQVEILKLENSLNSARKRLGEIRKYSYVDDEDEE +>1173061_clib918_MATdc_A|gene_index=1|name=MATA|role=core_MAT +MNSFLETPALILYSRKSQLPKKSEKSENVMKRIFSPDSFPSSTSKFNDPREKKKIMSDFLFYDGANNTFSETQAPPRKRKNSTEDKPIPRPTNSFICARSMLHKMFERLNISTIECSNLMSDIWKNYNDDLFKHYFRYLSFLEACWHTSVYPNYRYNPRKKLPSPKPSSCALKKYSKSIIKKRPVKTRAKSSGSTNMMPIKINSCTPGFSFNTEAPRRNYELEIIISELMKEEQLYFGMPIIETAPTSVNCKPFQIDDFSDNFLAFSYLYDEGMRDMSGAR +>4952_clib122_MATyl_B|gene_index=0|name=apn2|role=flanking_conserved +MRFVTFNICGINNVLSYHPWNEQRSFEHMFAVLKADVICLQETKLQPHLLKREHALVPGYDSYWSFSNTKKGYSGVAVYVKHGIKVLKAEEGLTGQLVSPDSKKGLTYQQLYERQRERDGSSKSCIGAYPEGNPQRLFQVDSEGRSLVLDLGFCVVFGLYCPSTATEGEERDAYRDDYFGLLEERVNLLVEAGREVVVMGDLNVARELYDSAEGMQALHKAKHITLPTLGDKEEGRFVEVFEKLNPGQCKSWREAKVGRRVFHRLVPDLLQDSCRNKHPNRPDMYTCWNVMMNYRPGNFGSRIDYVLASKGLKVEDADILPHLEGSDHCPVYAEVREPDACQEKDNRATQGAANQTKQASSSAPNSFRPKLFHRTNKQFVQTTSVMSMFRAQTPPTKGITKPKAPSKSTAKKQAAISSFFQKPDPEAAVVSTCVSDVHTKPLGQSQSPAVSPNIESTYQGCDAGAHATREGEDTRPQMATRLDLPEPTEYLDIAERVEEAEKVASQWSKIMTKKPAPVCAHNLPAVLRTTRKKGPNQGKHFWVCGQNHSLQLVGDSYKASADPDAKGSQDDTSARCNFFQWK +>4952_clib122_MATyl_B|gene_index=1|name=matb2|role=core_MAT +MKETRDLLFYNENSIDFNFSPEEMDTHQMMCVNNDSQQDTSFLTATTCIFDFQKDIYDDQNLMSFDPFFPQQRDMALWIASVGNGAIDWSTKPQLTLSKKIPDVVNEKGEEITPADLQRHYADKHVAKLPDHSCDLYYLLFKHAKFEATTLRIPLSLLNDNGTGALGPFDEIRSLTDEEQRLLTLLKTISHYSWVTKHATKDCTGQLLDLCKEGLQLVESIQVNKTFNGVILHILVAYFGRRSTALRDEFQLHKLFLEQRLDIELEILAQHGGADAIDSEKLRKVKKLSELWEWFNAIPYNHHPSVSQIEFIASQIGEKVSFVKSWVANKRRTGAKKRSKKPAPSTQIRPALKVFLEKKPFVSRQVV +>4952_clib122_MATyl_B|gene_index=2|name=matb1|role=core_MAT +MRQDEYHIKQRIFSISKAAITMIKIKTEHKVVKARTPKKMKPGMARFRIQAVPIINQNNNAYLSEISFEEKLEPVPPMYPELQKVLDYIHSRKVPFDGSSGPYPPSSRRQVVNGYVAFKLYHHVPHTDLSAIDITHLLQGPWRACPDRHIWTKYADHYRSRGRDVAFKTWLLSVTSPAPEKGLIIQPQTQSEQGYNFSSSSDFSSSPEHSETLPVSSGEPSTPVDPFLIEFDQGIAEKEASFTSPCNQNYDLDFSQLQPLSIRVPYFNQYASSSNSGLDVDYFDGSTLDTPSYIDTFITPLH +>4952_clib122_MATyl_B|gene_index=3|name=sla2|role=flanking_conserved +MSSNRAEVDLNVNIKKATNTDESAPKRKHVRACIVYTHDHRSSKAFWNGIRIQPLQTDDVMVFKALITIHKVLQEGHPSALRDAQANVNFIQSLARGNNYTGPNQGNHGYGKLISEYVRFLMQKLTFHKYHPAFNGMFEYEDYISLRTVNDPNEGYEAIMNLMTLQDGVDDFQRLVFSSLQRSRSNECKISSLVPMIAESYGIYRFVISMLRAMHVSTGNDEALESLRQRFYSQHDRLRDFYYDCSTLKYLRSIVTIPQLGKNPPNLLEEEDGPGLPQRPKSVAETRSVSPEPAPLPVATPTPSIPAEAQPIENFWSQDALLAQQQYDAEQERLRQQQAMEEERIRQMQMQQQQQFEMQQRQQMEAQQRAQEQLMADQMARHAQGRMAELERDILALRGQYDQDQLMLEQYDGRVKALEAELNQLQQTAHQSAQAKDDLIESLQQQITMWRQKYETLAKRYSSMREEYLALLKKLKATQQKAASAKEAIEKAEKLERDMRHKNIELADLIKERDRARYDLDRAKGGNKEDVERLERELRMAQDKLADKDRSTGADLSLLLSKHNRELSELENALKMKQRALDERGDDSDLLRRLEEKEIEYEALNEAFNSLALHQEQQGNNSNGGSTPLAALHSIIDALLESGSQRVQDALFELESPMQAGNQNSTPEYLLSVIEKASESASAFATSFNNFLADETDGDYAEIIKTVNIYSTAVENVLSNSKGLTRLAKDDASADALVNFARESAEATERNFIGLLSEIIEDFPLDDKMERVITLNMNVQTALQHLTKLAERMAPKTNINMSGDLGDLVEREMAKAADAIAAASAKLGDLLKFNQSDPLKSTTDLQLHEAAIQAAQAVINAIAALIRAATDAQNEIVAQGRGTSSRAQFYKKNNKWTEGLISAAKSVAASTNILIEKADGTLRRTSGLEELIVASNNVAASTAQLVAASRVKATFMSKTQDKLEECSKVVTSACRNLVKQVQEILNKKFGELDEKVDYAALSKHEFKTTEMEQQVEILKLENDLQGARKRLGQMRKVAYLHQDEEESIPGQED +>4952_w29_MATyl_A|gene_index=0|name=sla2|role=flanking_conserved +MSSNRAEVDLNVNIKKATNTDESAPKRKHVRACIVYTHDHRSSKAFWNGIRIQPLQTDDVMVFKALITIHKVLQEGHPSALRDAQANVNFIQSLARGNNYTGPNQGNHGYGKLISEYVRFLMQKLTFHKYHPAFNGMFEYEDYISLRTVNDPNEGYEAIMNLMTLQDGVDDFQRLVFSSLQRSRSNECKISSLVPMIAESYGIYRFVISMLRAMHVSTGNDEALESLRQRFYSQHDRLRDFYYDCSTLKYLRSIVTIPQLGKNPPNLLEEEDGPGLPQRPKSVAETRSVSPEPAPLPVATPTPSIPAEAQPIENFWSQDALLAQQQYDAEQERLRQQQAMEEERIRQMQMQQQQQFEMQQRQQMEAQQRAQEQLMADQMARHAQGRMAELERDILALRGQYDQDQLMLEQYDGRVKALEAELNQLQQTAHQSAQAKDDLIESLQQQITMWRQKYETLAKRYSSMREEYLALLKKLKATQQKAASAKEAIEKAEKMERDMRHKNIELADLIKERDRARYDLDRAKGGNKEDVERLERELRMAQDKLADKDRSTGADLSLLLSKHNRELSELENALKMKQRALDERGDDSDLLRRLEEKEIEYEALNEAFNSLALHQEQQGNNSNGGSTPLAALHSIIDALLESGSQRVQDALFELESPMQAGNQNSTPEYLLSVIEKASESASAFATSFNNFLADETDGDYAEIIKTVNIYSTAVENVLSNSKGLTRLAKDDASADALVNFARESAEATERNFIGLLSEIIEDFPLDDKMERVITLNMNVQTALQHLTKLAERMAPKTNINMSGDLGDLVEREMAKAADAIAAAASAKLGDLLKFNQSDPLKSTTDLQLHEAAIQAAQAVINAIAALIRAATDAQNEIVAQGRGTSSRAQFYKKNNKWTEGLISAAKSVAASTNILIEKADGTLRRTSGLEELIVASNNVAASTAQLVAASRVKATFMSKTQDKLEECSKVVTSACRNLVKQVQEILNKKFGELDEKVDYAALSKHEFKTTEMEQQVEILKLENDLQGARKRLGQMRKVAYLHQDEEESIPGQED +>4952_w29_MATyl_A|gene_index=1|name=mata1|role=core_MAT +MPSRTPTDIWRCQRLILAARKGETTCQALHEQSIEISSSLKWFEEISEWMSKFPESGLYISTTIWTFLEGVPNEHRIKYDRLIRHYITYIQQLVPARLVTAYLDSLFKICSHPTRTERRIIGEHCGISLHQVTQWFTNRRFREPKTDSPDDGSTRFIPSPMTSPGSIDECSFPESP +>4952_w29_MATyl_A|gene_index=2|name=mata2|role=core_MAT +MENTILHIHSFQLPQTEQPYPEAMLFDRDTSDSRTVLTQKPNGLEISLNFLQYDGHKGLFIRQDSRTNEPEYIEPKVLKPRNSYILFRNATSRCSQSIDPNSAFVSRVSSYIWGSGIPNPIVRRWFKYMGFFEALYHEVDYPEYIPNKLPKSPKKPKVQKGATKSKKKGAKGKNKVTALQVLVGPTVGVGGRMTVSPMTAFFSNNSHVTCYDPNFVLDTPTREFLMMPLDDITLPSQGPRSDSQEQHVPRQPPDGQDYFDILDIDDFVSPYDDLTTRSFTNRQLFKHATLG +>4952_w29_MATyl_A|gene_index=3|name=apn2|role=flanking_conserved +RDAYRDDYFGLLEERVNLLVEAGREVVVMGDLNVARELYDSAEGMQALHKAKHITLPTLGDKEEGRFVEVFEKLNPGQCKSWREAKVGRRVFHRLVPDLLQDSCRNKHPNRPDMYTCWNVMMNYRPGNFGSRIDYVLASKGLKVEDADILPHLEGSDHCPVYAEVREPDACQEKDNRATQGAANQTKQASSSAPNSFRPKLFHRTNKQFVQTTSVMSMFRAQTPPTKGITKPKAPSKSTAKKQAAISSFFQKPDPEAAVVSTCVSDVHTKPLGQSQSPAVSPNIESTYQGCDAGAHATREGEDTRPQMATRLDLPEPTEYLDIAERVEEAEKVASQWSKIMTKKPAPVCAHNLPAVLRTTRKKGPNQGKHFWVCGQNHSLQLVGDSYKASADPDAKGSQDDTSARCNFFQWK +>ONH67106.1 Mating-type-like protein ALPHA1 [Petasospora fabianii] +MTHDVLSQYIKNHSLVSSFNGGMKVHDVIVEALSFIKEELCFATQYFNITQLILEASQFSLREAMKPLDL +QVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPPSQLVWQRFAAEYNQT +VRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYH +NYLKERLQSAIDRPIVTLASDPSLSRSYIDPLVYEISSGTYEKS + +>XP_020071302.1 HMG-box domain-containing protein CYBJADRAFT_71620 [Williopsis jadinii] +MSKQRMLAKVVLNDAKHTISALSQVKVTPPVFTREYNDMGNGRSEIIKSLIDDLVKDISRVSIGIQALQV +NLNLDVSPIQFSTRPVRKKTLYNTWYKDNFSNCKEEHPEWDATRITKKLVRDWREFSDDDKEALEIEYGF +KSKTE + diff --git a/results/2026-10-30_petasospora/data/aug_candida_guilliermondii.gff3 b/results/2026-10-30_petasospora/data/aug_candida_guilliermondii.gff3 new file mode 100644 index 00000000..40dadb14 --- /dev/null +++ b/results/2026-10-30_petasospora/data/aug_candida_guilliermondii.gff3 @@ -0,0 +1,1557 @@ +##gff-version 3 +# This output was generated with AUGUSTUS (version 3.5.0). +# AUGUSTUS is a gene prediction tool written by M. Stanke (mario.stanke@uni-greifswald.de), +# O. Keller, S. König, L. Gerischer, L. Romoth, Katharina Hoff, Henry Mehlan and Daniel Honsel. +# Please cite: Mario Stanke, Mark Diekhans, Robert Baertsch, David Haussler (2008), +# Using native and syntenically mapped cDNA alignments to improve de novo gene finding +# Bioinformatics 24: 637-644, doi 10.1093/bioinformatics/btn013 +# No extrinsic information on sequences given. +# Sources of extrinsic information: M RM +# Initializing the parameters using config directory /opt/linux/centos/8.x/x86_64/pkgs/augustus/3.5.0/env/config/ ... +# candida_guilliermondii version. Using default transition matrix. +# Looks like windows.fa is in fasta format. +# We have hints for 0 sequences and for 0 of the sequences in the input set. +# +# ----- prediction on sequence number 1 (length = 19001, name = pf_a_cp048715) ----- +# +# Predicted genes for sequence number 1 on both strands +# start gene g1 +pf_a_cp048715 AUGUSTUS gene 746 1603 0.7 - . ID=g1 +pf_a_cp048715 AUGUSTUS transcript 746 1603 0.7 - . ID=g1.t1;Parent=g1 +pf_a_cp048715 AUGUSTUS stop_codon 746 748 . - 0 Parent=g1.t1 +pf_a_cp048715 AUGUSTUS CDS 749 1603 0.7 - 0 ID=g1.t1.cds;Parent=g1.t1 +pf_a_cp048715 AUGUSTUS start_codon 1601 1603 . - 0 Parent=g1.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagaccagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcggaatacattagggcacaggatttcaagtatatcgagacgatttcagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# agaagagattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgatgaagatgac +# accagtgaagacacagagcgactcgtttctgacgctgctgatattatctggccaaagtcctatgattctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgacctcgctcgactattcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEDQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETISDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVKKRFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSDAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g1 +### +# start gene g2 +pf_a_cp048715 AUGUSTUS gene 1950 2876 1 - . ID=g2 +pf_a_cp048715 AUGUSTUS transcript 1950 2876 1 - . ID=g2.t1;Parent=g2 +pf_a_cp048715 AUGUSTUS stop_codon 1950 1952 . - 0 Parent=g2.t1 +pf_a_cp048715 AUGUSTUS CDS 1953 2876 1 - 0 ID=g2.t1.cds;Parent=g2.t1 +pf_a_cp048715 AUGUSTUS start_codon 2874 2876 . - 0 Parent=g2.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# cagcacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccagatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttgtctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggagtgctcatgacctcgtcacaggttgtcacctatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgagcaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaatactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVTARQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILSTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g2 +### +# start gene g3 +pf_a_cp048715 AUGUSTUS gene 3351 3743 0.95 - . ID=g3 +pf_a_cp048715 AUGUSTUS transcript 3351 3743 0.95 - . ID=g3.t1;Parent=g3 +pf_a_cp048715 AUGUSTUS stop_codon 3351 3353 . - 0 Parent=g3.t1 +pf_a_cp048715 AUGUSTUS CDS 3354 3743 0.95 - 0 ID=g3.t1.cds;Parent=g3.t1 +pf_a_cp048715 AUGUSTUS start_codon 3741 3743 . - 0 Parent=g3.t1 +# coding sequence = [atgtcagttatctttttgaacgatccggaaaactcaatcaagcagagtaagcaaaagaccgaaaaagcttcagctagtg +# atatcaaagatatgagcagttatgtcaagctttgcactagtgtttatgaggaagacgactgcaaacggaggggggtcgatgctctccacgattcagat +# gactacaaagaccatgtgcttgtattaacgcttaacgagcctaatatgaaagctccggttgactctgctagtacaaacgccaggacaacatataaggt +# aacaaagcccacaaaaaagaagaagacgccgcctcatttggagcttgtattcagtaaattcaggtcccctttgtatggtgaagccgaggatatttgca +# tcaacagttcccctatgtga] +# protein sequence = [MSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAP +# VDSASTNARTTYKVTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g3 +### +# start gene g4 +pf_a_cp048715 AUGUSTUS gene 5742 6971 1 - . ID=g4 +pf_a_cp048715 AUGUSTUS transcript 5742 6971 1 - . ID=g4.t1;Parent=g4 +pf_a_cp048715 AUGUSTUS stop_codon 5742 5744 . - 0 Parent=g4.t1 +pf_a_cp048715 AUGUSTUS CDS 5745 6971 1 - 0 ID=g4.t1.cds;Parent=g4.t1 +pf_a_cp048715 AUGUSTUS start_codon 6969 6971 . - 0 Parent=g4.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacgagacaagagctcgagaagacggtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcacaggaatcaccgacgactcccagacatccaaacta +# cagctcatctactatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggctcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaactag] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLTGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g4 +### +# start gene g5 +pf_a_cp048715 AUGUSTUS gene 7276 7797 1 + . ID=g5 +pf_a_cp048715 AUGUSTUS transcript 7276 7797 1 + . ID=g5.t1;Parent=g5 +pf_a_cp048715 AUGUSTUS start_codon 7276 7278 . + 0 Parent=g5.t1 +pf_a_cp048715 AUGUSTUS CDS 7276 7794 1 + 0 ID=g5.t1.cds;Parent=g5.t1 +pf_a_cp048715 AUGUSTUS stop_codon 7795 7797 . + 0 Parent=g5.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g5 +### +# start gene g6 +pf_a_cp048715 AUGUSTUS gene 8035 9825 0.64 - . ID=g6 +pf_a_cp048715 AUGUSTUS transcript 8035 9825 0.64 - . ID=g6.t1;Parent=g6 +pf_a_cp048715 AUGUSTUS stop_codon 8035 8037 . - 0 Parent=g6.t1 +pf_a_cp048715 AUGUSTUS CDS 8038 9825 0.64 - 0 ID=g6.t1.cds;Parent=g6.t1 +pf_a_cp048715 AUGUSTUS start_codon 9823 9825 . - 0 Parent=g6.t1 +# coding sequence = [atgaagaaggcaagatccaccatgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccgggac +# agcacgctgatggctcggacgtcagctcggatgctgcgcagtccttcatcggtggctcaagtgtcgtttcggcacatcctgaacatcaccaccaggag +# acttcatccaaggacgagcagctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcag +# tcttccatttggtggaaatgcgatacacttcccatcaataaagcagattagacagaatcttcctatcattggcagcaacgatacagatgatcgagggt +# atgaccctactctttcaaggcatgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaag +# ggtgttgacaacggtaggttcagagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatcagt +# gtgggaccagattgaaggtgaggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctt +# tcaagacagggctcaatttgaacaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagaca +# ctaaatcatgttggaccagttgatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagact +# gtcctgcgatataagttcacagaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcact +# caatggggggcttggttgcccaccatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtg +# ctcggtccaatccgctatggtgatactgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgaggtccagttttgtcttcctacc +# tgaggatggccgctgcttcatcgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccag +# cagtggctaagagccgccagaaagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaa +# cgttctctctctttgaggtcctctcacaagtcgacagcagaagaatttaacgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaag +# aaccaaaaagttcctcgatgaccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtg +# cccgtgttgatggtgtcgctgatatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgtgcatcataagtggttgatgcca +# gagagaagaggattccctgttgcggccaaactttcaagtggtgagggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgc +# agtgctacaggaagagaaagcaagactatctgctagagatggataa] +# protein sequence = [MKKARSTMSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLE +# SSHYKNEHKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAIS +# IPSLIDEKRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTN +# GKTNVHTFGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTP +# EINFFMRSSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVS +# FDESYAYLSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHIS +# LMTDLETMGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g6 +### +# start gene g7 +pf_a_cp048715 AUGUSTUS gene 10516 11175 0.94 + . ID=g7 +pf_a_cp048715 AUGUSTUS transcript 10516 11175 0.94 + . ID=g7.t1;Parent=g7 +pf_a_cp048715 AUGUSTUS start_codon 10516 10518 . + 0 Parent=g7.t1 +pf_a_cp048715 AUGUSTUS CDS 10516 11172 0.94 + 0 ID=g7.t1.cds;Parent=g7.t1 +pf_a_cp048715 AUGUSTUS stop_codon 11173 11175 . + 0 Parent=g7.t1 +# coding sequence = [atgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggac +# ctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaag +# gatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagttt +# ggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcgacgttacagagggtacaccattgc +# acagagtcgccgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttaaagtttgatggc +# tgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggt +# tgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNS +# RVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFII +# KSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g7 +### +# start gene g8 +pf_a_cp048715 AUGUSTUS gene 11319 12716 1 - . ID=g8 +pf_a_cp048715 AUGUSTUS transcript 11319 12716 1 - . ID=g8.t1;Parent=g8 +pf_a_cp048715 AUGUSTUS stop_codon 11319 11321 . - 0 Parent=g8.t1 +pf_a_cp048715 AUGUSTUS CDS 11322 12716 1 - 0 ID=g8.t1.cds;Parent=g8.t1 +pf_a_cp048715 AUGUSTUS start_codon 12714 12716 . - 0 Parent=g8.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggtgacgctcgaggaagtggtagaggagaaggaagaggtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaatgtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatttttgtcggtcttgtga +# ccggtaagatctctgttgttatcaagggcggtgatggtgcctttgccaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggcatcatctcactctggtccaccacagacctatcattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccatcttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRGNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHECMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFANACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLSLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g8 +### +# start gene g9 +pf_a_cp048715 AUGUSTUS gene 12921 14789 0.92 + . ID=g9 +pf_a_cp048715 AUGUSTUS transcript 12921 14789 0.92 + . ID=g9.t1;Parent=g9 +pf_a_cp048715 AUGUSTUS start_codon 12921 12923 . + 0 Parent=g9.t1 +pf_a_cp048715 AUGUSTUS CDS 12921 14786 0.92 + 0 ID=g9.t1.cds;Parent=g9.t1 +pf_a_cp048715 AUGUSTUS stop_codon 14787 14789 . + 0 Parent=g9.t1 +# coding sequence = [atgaattcccaacggcatacaccctatattcgagctgactcctccagtgtgctaccgaggacgctgggccgtcgaccgc +# ccggccctgtcatcccgaacctgggagctaagcaccatcacagctctcttaacaaaccccctcgatcatctcgacagcatgatgaacgagagtcttca +# tcacctcccagaccacacagctcctttgccccacctatcaactccaattcctcaaccacgtccgcctctagtcccaaaaaaaacgttaccgatgggcg +# ggaccctgccaacttgatctccacaacgtacgcctactgcggcaacaggaaacgactgcagaatccaagggatgtcgaaaataaaaagagactaagag +# agatggaagcaaaaaatgctcctggggaaccgccggtggctctacagtctaaaaggtttcaacaggaagcgtcagttgtcaaggcgaatgatggggac +# acctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctttggagaaacgtgccccaat +# aatgtctccaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcgatcagacgctgacttcatgaactccattcgtgctccaagga +# tccccagtcgagctgctcaagctgactacgagaagcacactgctagcgctcgtctacaacataaacgtgaggatacagaggtcaagggaatggatcaa +# caattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcgctgattaaaggtgtgaa +# atcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtcagggagtaagtcacctc +# accgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccggggccgtcatgaaggttcagtgggggacgctctaggc +# tcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaaggatgatgaaagtcataaagc +# tatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatcttgagatgatgaagcgca +# ggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatgagtttgaagaggttgac +# gagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagtttccgggaacattaaccctcggtgagattgagcaaaa +# ggtcaaccagcacatggactgcattccaaaaattctcaacggtcgtcttgactccacttacctgcaaaaagcgaaagtattcatgcacgactctggag +# caacaatcttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaaaagacaaggggctattgctaatcta +# atcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattcacattacacattctcgc +# tccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagttgattatggtcaataca +# tcgctgatttggtcccttgtgaatga] +# protein sequence = [MNSQRHTPYIRADSSSVLPRTLGRRPPGPVIPNLGAKHHHSSLNKPPRSSRQHDERESSSPPRPHSSFAPPINSNSST +# TSASSPKKNVTDGRDPANLISTTYAYCGNRKRLQNPRDVENKKRLREMEAKNAPGEPPVALQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNR +# KRNILSSLEKRAPIMSPGEEEQEGSRHRIPSIRSDADFMNSIRAPRIPSRAAQADYEKHTASARLQHKREDTEVKGMDQQLHDDTNSGKRETKKGEDV +# APIPRKRALIKGVKSSAKKSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRGRHEGSVGDALGSDEELFNFDSPGPSSQELR +# SAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNE +# EFPGTLTLGEIEQKVNQHMDCIPKILNGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVI +# QFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g9 +### +# start gene g10 +pf_a_cp048715 AUGUSTUS gene 15247 18372 1 + . ID=g10 +pf_a_cp048715 AUGUSTUS transcript 15247 18372 1 + . ID=g10.t1;Parent=g10 +pf_a_cp048715 AUGUSTUS start_codon 15247 15249 . + 0 Parent=g10.t1 +pf_a_cp048715 AUGUSTUS CDS 15247 18369 1 + 0 ID=g10.t1.cds;Parent=g10.t1 +pf_a_cp048715 AUGUSTUS stop_codon 18370 18372 . + 0 Parent=g10.t1 +# coding sequence = [atgtcgaagacaatctccgactccgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctatggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgccgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctctaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagttagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaaaaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaaaaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacctgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacatcaatggtgatcttggtgatgcagttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctattgccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgaccaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcttccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagtgaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacgtctgcctgcagatctttggtcaacca +# agttcaggatattttgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagatcagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAIAVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 18 +# RM: 18 +# end gene g10 +### +# +# ----- prediction on sequence number 2 (length = 19501, name = pf_a_jcm3601) ----- +# +# Predicted genes for sequence number 2 on both strands +# start gene g11 +pf_a_jcm3601 AUGUSTUS gene 944 1801 0.78 - . ID=g11 +pf_a_jcm3601 AUGUSTUS transcript 944 1801 0.78 - . ID=g11.t1;Parent=g11 +pf_a_jcm3601 AUGUSTUS stop_codon 944 946 . - 0 Parent=g11.t1 +pf_a_jcm3601 AUGUSTUS CDS 947 1801 0.78 - 0 ID=g11.t1.cds;Parent=g11.t1 +pf_a_jcm3601 AUGUSTUS start_codon 1799 1801 . - 0 Parent=g11.t1 +# coding sequence = [atgattagtactaacagtcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctgaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgacgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgaatctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNSHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYESINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g11 +### +# start gene g12 +pf_a_jcm3601 AUGUSTUS gene 2148 3074 0.91 - . ID=g12 +pf_a_jcm3601 AUGUSTUS transcript 2148 3074 0.91 - . ID=g12.t1;Parent=g12 +pf_a_jcm3601 AUGUSTUS stop_codon 2148 2150 . - 0 Parent=g12.t1 +pf_a_jcm3601 AUGUSTUS CDS 2151 3074 0.91 - 0 ID=g12.t1.cds;Parent=g12.t1 +pf_a_jcm3601 AUGUSTUS start_codon 3072 3074 . - 0 Parent=g12.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtgg +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttttctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggttgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctggt +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVAPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g12 +### +# start gene g13 +pf_a_jcm3601 AUGUSTUS gene 3549 3941 0.94 - . ID=g13 +pf_a_jcm3601 AUGUSTUS transcript 3549 3941 0.94 - . ID=g13.t1;Parent=g13 +pf_a_jcm3601 AUGUSTUS stop_codon 3549 3551 . - 0 Parent=g13.t1 +pf_a_jcm3601 AUGUSTUS CDS 3552 3941 0.94 - 0 ID=g13.t1.cds;Parent=g13.t1 +pf_a_jcm3601 AUGUSTUS start_codon 3939 3941 . - 0 Parent=g13.t1 +# coding sequence = [atgtcagttatctttttgaacgatccggaaaactcaatcaagcagagtaagcaaaagaccgaaaaagcttcagctagtg +# atatcaaagatatgagcagttatgtcaagctttgcactagtgtttatgaggaagacgactgcaaacggaggggggtcgatgctctccacgattcagat +# gactacaaagaccatgtgcttgtattaacgcttaacgagcctaatatgaaagctccggttggctctgctagtacaaacgccaggacaacatataaggt +# aacaaagcccacaaaaaagaagaagacgccgcctcatttggagcttgtattcagtaaattcaggtcccctttgtatggtgaagccgaggatatttgca +# tcaacagttcccctatgtga] +# protein sequence = [MSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAP +# VGSASTNARTTYKVTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g13 +### +# start gene g14 +pf_a_jcm3601 AUGUSTUS gene 5937 7166 1 - . ID=g14 +pf_a_jcm3601 AUGUSTUS transcript 5937 7166 1 - . ID=g14.t1;Parent=g14 +pf_a_jcm3601 AUGUSTUS stop_codon 5937 5939 . - 0 Parent=g14.t1 +pf_a_jcm3601 AUGUSTUS CDS 5940 7166 1 - 0 ID=g14.t1.cds;Parent=g14.t1 +pf_a_jcm3601 AUGUSTUS start_codon 7164 7166 . - 0 Parent=g14.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctactatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggctcttggtgctattgtcaaaagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaactag] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g14 +### +# start gene g15 +pf_a_jcm3601 AUGUSTUS gene 7472 7993 1 + . ID=g15 +pf_a_jcm3601 AUGUSTUS transcript 7472 7993 1 + . ID=g15.t1;Parent=g15 +pf_a_jcm3601 AUGUSTUS start_codon 7472 7474 . + 0 Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS CDS 7472 7990 1 + 0 ID=g15.t1.cds;Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 7991 7993 . + 0 Parent=g15.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgaaatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g15 +### +# start gene g16 +pf_a_jcm3601 AUGUSTUS gene 8231 10021 0.62 - . ID=g16 +pf_a_jcm3601 AUGUSTUS transcript 8231 10021 0.62 - . ID=g16.t1;Parent=g16 +pf_a_jcm3601 AUGUSTUS stop_codon 8231 8233 . - 0 Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS CDS 8234 10021 0.62 - 0 ID=g16.t1.cds;Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS start_codon 10019 10021 . - 0 Parent=g16.t1 +# coding sequence = [atgaagaaggcaagatccaccatgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggac +# agcacgctgatggctcggacgtcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcacgaccaggag +# acttcatccaaggacgagcagctggagaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcag +# tcttccatttggtggaaatgcgatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggt +# atgaccctactctttcaaggcatgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaag +# ggtgttgacaacggtaggttcagagcagtgaagaaggcatttacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgt +# gtgggaccagattgaaggtgaggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctt +# tcaagacagggctcaatttgaacaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagaca +# ctaaatcatgttggaccagtcgatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagact +# gtcctgcgatataagttcacagaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcact +# caatggggggcttggttgcccaccatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtg +# ctcggtccaatccgctatggtgataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacc +# tgaggatggccgctgcttcatcgacaagaaaacccatgaacagtacaaccttgactattttgatccgcaaacttgggtggagtataacttgtcaccag +# cagtggctaagagccgccagaaagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaa +# cgttctctctctttgaggtcctctcacaagtcgacagcagaagaatttaatgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaag +# aaccaaaaagttcctcgatgaccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtg +# cccgtgttgatggtgtcgctgatatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgtgcatcataagtggttgatgcca +# gagagaagaggattccctgttgcggccaaactttcaagtggtgagggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgc +# agtgctacaggaagagaaagcaagactatctgctagagatggataa] +# protein sequence = [MKKARSTMSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHDQETSSKDEQLENNQVTTDDLE +# SSHYKNEHKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAIS +# IPSLIDEKRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTN +# GKTNVHTFGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTP +# EINFFMRSSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVS +# FDESYAYLSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHIS +# LMTDLETMGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g16 +### +# start gene g17 +pf_a_jcm3601 AUGUSTUS gene 10711 11370 0.97 + . ID=g17 +pf_a_jcm3601 AUGUSTUS transcript 10711 11370 0.97 + . ID=g17.t1;Parent=g17 +pf_a_jcm3601 AUGUSTUS start_codon 10711 10713 . + 0 Parent=g17.t1 +pf_a_jcm3601 AUGUSTUS CDS 10711 11367 0.97 + 0 ID=g17.t1.cds;Parent=g17.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 11368 11370 . + 0 Parent=g17.t1 +# coding sequence = [atgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggac +# ctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaag +# gatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagttt +# ggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcgacgttacagagggtacaccattgc +# acagagtcgccgaggagggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttgaagtttgatggc +# tgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggt +# tgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNS +# RVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEEGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFII +# KSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g17 +### +# start gene g18 +pf_a_jcm3601 AUGUSTUS gene 11514 12911 1 - . ID=g18 +pf_a_jcm3601 AUGUSTUS transcript 11514 12911 1 - . ID=g18.t1;Parent=g18 +pf_a_jcm3601 AUGUSTUS stop_codon 11514 11516 . - 0 Parent=g18.t1 +pf_a_jcm3601 AUGUSTUS CDS 11517 12911 1 - 0 ID=g18.t1.cds;Parent=g18.t1 +pf_a_jcm3601 AUGUSTUS start_codon 12909 12911 . - 0 Parent=g18.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggcgacgctcgaggaagtggtagaggggaaggaagaagtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatatttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccaccttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRSNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g18 +### +# start gene g19 +pf_a_jcm3601 AUGUSTUS gene 13758 14984 0.47 + . ID=g19 +pf_a_jcm3601 AUGUSTUS transcript 13758 14984 0.47 + . ID=g19.t1;Parent=g19 +pf_a_jcm3601 AUGUSTUS start_codon 13758 13760 . + 0 Parent=g19.t1 +pf_a_jcm3601 AUGUSTUS CDS 13758 14981 0.47 + 0 ID=g19.t1.cds;Parent=g19.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 14982 14984 . + 0 Parent=g19.t1 +# coding sequence = [atgaactccattcgtgctccaaggattcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtc +# tacaacataaacgtgaggatacagaggtcaaggaaatggatcaacaattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagat +# gtcgctccaataccaaggaagagggcgctgattaaaggtgtgaaatcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattag +# gggcatgctgtctggaaagaaaaagtcagggagtaagtcacctcaccgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacaca +# agcaccgggaccgtcatgaaggttcagtgggggacgctctaggctcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattg +# cgttccgccaaagagcttctcaaggatgatgaaagtcataaagctatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacg +# attcaaacccgatggtgatgactatcttgagatgatgaaacgcaggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatc +# aggatatcagtgttggtctacaaaatgagtttgaagaggttgacgagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaat +# gaggagttcccgggaacattaagcctcggtgagattgagcaaaaggccaaccagcacatggactgcattccaaaaattctcgacggtcgtcttgactc +# cacttacctacaaaaagcgaaagtattcatgcacgactctggagcaacaattttgtccaatgcagatctccaaagacctagtttcaagcagttcttta +# gtggattttatggtttgaagaggcaaggggctattgccaatctgatcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggtt +# atccaattttggtccatcgatacattcacattacacattctcgctccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatga +# ggctatcagagtgttggatagaacagttgattatggtcaatacatcgctgatttggtcccttgtgaatga] +# protein sequence = [MNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRETKKGEDVAPIPRKRALIKGVKSSAK +# KSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGPSSQELRSAKELLKDDESHKAMKSA +# IDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNEEFPGTLSLGEIEQKANQH +# MDCIPKILDGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVIQFWSIDTFTLHILAPEVA +# ARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 5 +# RM: 5 +# end gene g19 +### +# start gene g20 +pf_a_jcm3601 AUGUSTUS gene 15442 18567 1 + . ID=g20 +pf_a_jcm3601 AUGUSTUS transcript 15442 18567 1 + . ID=g20.t1;Parent=g20 +pf_a_jcm3601 AUGUSTUS start_codon 15442 15444 . + 0 Parent=g20.t1 +pf_a_jcm3601 AUGUSTUS CDS 15442 18564 1 + 0 ID=g20.t1.cds;Parent=g20.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 18565 18567 . + 0 Parent=g20.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatatggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagcagctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatgttgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDVDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g20 +### +# +# ----- prediction on sequence number 3 (length = 22001, name = pf_alpha_strain65) ----- +# +# Predicted genes for sequence number 3 on both strands +# start gene g21 +pf_alpha_strain65 AUGUSTUS gene 704 1561 0.81 - . ID=g21 +pf_alpha_strain65 AUGUSTUS transcript 704 1561 0.81 - . ID=g21.t1;Parent=g21 +pf_alpha_strain65 AUGUSTUS stop_codon 704 706 . - 0 Parent=g21.t1 +pf_alpha_strain65 AUGUSTUS CDS 707 1561 0.81 - 0 ID=g21.t1.cds;Parent=g21.t1 +pf_alpha_strain65 AUGUSTUS start_codon 1559 1561 . - 0 Parent=g21.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgatgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgactctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g21 +### +# start gene g22 +pf_alpha_strain65 AUGUSTUS gene 1908 2834 0.99 - . ID=g22 +pf_alpha_strain65 AUGUSTUS transcript 1908 2834 0.99 - . ID=g22.t1;Parent=g22 +pf_alpha_strain65 AUGUSTUS stop_codon 1908 1910 . - 0 Parent=g22.t1 +pf_alpha_strain65 AUGUSTUS CDS 1911 2834 0.99 - 0 ID=g22.t1.cds;Parent=g22.t1 +pf_alpha_strain65 AUGUSTUS start_codon 2832 2834 . - 0 Parent=g22.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttttctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g22 +### +# start gene g23 +pf_alpha_strain65 AUGUSTUS gene 3495 5891 0.87 + . ID=g23 +pf_alpha_strain65 AUGUSTUS transcript 3495 5891 0.87 + . ID=g23.t1;Parent=g23 +pf_alpha_strain65 AUGUSTUS start_codon 3495 3497 . + 0 Parent=g23.t1 +pf_alpha_strain65 AUGUSTUS CDS 3495 5888 0.87 + 0 ID=g23.t1.cds;Parent=g23.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 5889 5891 . + 0 Parent=g23.t1 +# coding sequence = [atgttgtcccaccgccatcagaaagctgctctctccaaactgcaaagaagtgcttacgcacattacccaacaccacatt +# cgccaatatctctaacaccaaatagatcaatatcgtctcttccggcctcgaagatgcttccagctgtcaattacgttcgccaagcagatccagatgcg +# attgttcttctcggtcatggtgaatcttaccatgctttcggcagagtcgatactaatctgattaaaggaatggtatcagctggcaatgcaagcgagat +# actctaccgtgatgatggactgacacaatggagattcaagtcatctcgactcccgcataacacttcttggctggtgaaggagttgaacaggccagttg +# tcaccttggaatggaacgaatatgctactagtttcctacagccattgcgcaacgactcccattggcatttcctatcgagaaccattcctgatggccac +# tcagaccatgaattctctgttcattctgaatctcaaaacatcatagtaattggtatacaggggaagattctcaaggggatcaaaacaggtgaaaagtc +# acagagacgtgttggtatcacttgctttgacccaacaaacggatcgatgtctgttcagggcacttctcttgctgatttgagatcccatatcgtgagct +# ttcgtcctcgggctattgttctggactccagtctgattgggacataccgggacatcattggcacagctcttctaggttcaccagaagttaagtttacc +# aatatgcctcccgtgttgggctcgctacaactccttaatgatttctttgatgtctctggtgaaatttggaggaagtgtttcgatgattacgagctgta +# ctcactgaaggctgctgtggagttcattaagaagacacagaaatcaacgtctgcagtattccaactaccatcgagggaactgtcgtcacaatggaccg +# tgttattggacaaagaaactatcagttccttgaacctgggctccaccgggagtcactatatgaattcctcctggttacttcaaagatcaatcaagact +# gctactgttggtggttctcgaatgttgtacaagtctatcttgaggccaactaggatagtcagtgagattttgtttcgtcagagggcggttcaatccat +# cattaaaaggaaacatacacgtcagaagctgagctcagagctctccagtttaccagacatcccattgctactacagaaactcaagatctatccggaat +# cagaagaccttgtcagtactatactacactccatcgacatcatctcgtcacttgcccccatcgcctccaagttttacatgaacggtcaccagcaagac +# gctgactttgatgatggtgacttgatttaccgcattccatctgcaaaacgtttggaggaactcagagacagggcccgttcccttctcaatgctagcga +# cacaatgcatggtgttgtttcctcgaatacaagcattgaatgcaatgatcggaggacacagaaagctactttggtgaatggtgttgattgtggaccta +# ataggaacacttctttttctcactctgtgaaaaatcagacaggtgatgtttctttggaaacctttatggtcagcgatgatgctgaattgaacactgag +# tcaccttctaatctcacaacagtgctagacacccgtactgtgagtgaacacacccagcctaaggggctaggagagattaccagctcatctttggacta +# ccgagatctacatgctacacaccgcatcgacaagttggccggtcagataagtgaattacaccctggccaccctctaccaagagccaaagtgattgcca +# actgctcggctgaaatattaagacataaggagctcattctactcttctcccgactcttgtcacgtttggacatgctgctgtcgtttgccaacgtggct +# atccaacatgacgacacaagacctatcgtggatcccagcagaaccaactttgagattcacttgaaggacaaagatttcaatgctgctgctgatccatt +# gttgatgtcaaactcatcatcagtattgtggactaagggagagagaaaaaccgtggcagagctttacgggctgtacgccagtatcatcttattggcgc +# actcaggtgcttatgttccagccaagtctgctaacattggtgttgtcagccacatgctttgtcaactccgctctacaaatcctttcctattacattca +# gcgcttgagaagtctatggatggccgtcgtatcatcatgtgcgacgctctgcctgacgatttcgaagactatggctcagccgatgacagattggaaag +# ctaccaagaaacactggcgactatgaatgtgggaactaaccgactgcttctaagatatcactga] +# protein sequence = [MLSHRHQKAALSKLQRSAYAHYPTPHSPISLTPNRSISSLPASKMLPAVNYVRQADPDAIVLLGHGESYHAFGRVDTN +# LIKGMVSAGNASEILYRDDGLTQWRFKSSRLPHNTSWLVKELNRPVVTLEWNEYATSFLQPLRNDSHWHFLSRTIPDGHSDHEFSVHSESQNIIVIGI +# QGKILKGIKTGEKSQRRVGITCFDPTNGSMSVQGTSLADLRSHIVSFRPRAIVLDSSLIGTYRDIIGTALLGSPEVKFTNMPPVLGSLQLLNDFFDVS +# GEIWRKCFDDYELYSLKAAVEFIKKTQKSTSAVFQLPSRELSSQWTVLLDKETISSLNLGSTGSHYMNSSWLLQRSIKTATVGGSRMLYKSILRPTRI +# VSEILFRQRAVQSIIKRKHTRQKLSSELSSLPDIPLLLQKLKIYPESEDLVSTILHSIDIISSLAPIASKFYMNGHQQDADFDDGDLIYRIPSAKRLE +# ELRDRARSLLNASDTMHGVVSSNTSIECNDRRTQKATLVNGVDCGPNRNTSFSHSVKNQTGDVSLETFMVSDDAELNTESPSNLTTVLDTRTVSEHTQ +# PKGLGEITSSSLDYRDLHATHRIDKLAGQISELHPGHPLPRAKVIANCSAEILRHKELILLFSRLLSRLDMLLSFANVAIQHDDTRPIVDPSRTNFEI +# HLKDKDFNAAADPLLMSNSSSVLWTKGERKTVAELYGLYASIILLAHSGAYVPAKSANIGVVSHMLCQLRSTNPFLLHSALEKSMDGRRIIMCDALPD +# DFEDYGSADDRLESYQETLATMNVGTNRLLLRYH] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g23 +### +# start gene g24 +pf_alpha_strain65 AUGUSTUS gene 7801 8718 0.76 + . ID=g24 +pf_alpha_strain65 AUGUSTUS transcript 7801 8718 0.76 + . ID=g24.t1;Parent=g24 +pf_alpha_strain65 AUGUSTUS start_codon 7801 7803 . + 0 Parent=g24.t1 +pf_alpha_strain65 AUGUSTUS CDS 7801 8715 0.76 + 0 ID=g24.t1.cds;Parent=g24.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 8716 8718 . + 0 Parent=g24.t1 +# coding sequence = [atgattgaagttcctctcggagcacttttggctcttagtgatccatacaccttctcttcacaaggtaaaatgaccggta +# tatcaaagatagtgaagaagcgaaacaaacaaggagttgaggttcttcgtttccgcggatgcccagcgtctaaagtgaggggcacaaggggtgcccaa +# ggactgcatcagtgtatggaaaggaacaattccatgaccagtggagaagcatccgtaacaatttttgctgctacgaagcttcctgatataccaagcat +# accgtatactttacagaagcagctggatgagctccgctctcagacaaccacatcagaccgcaggaagatcaaacgccttccccagctgcaagtgtcgg +# tgaacagagatgatacggttattaaaagggccaccaaaaccaaactaaatgggtttatggctttccgggcatttcattctcgtgaaatttctactatt +# gaacaccaaaggggtatttcgatgtcactggcgactgtgtgggcgagcgatcctccctcgcaacttgtctggcaaagattcgcagcagaatataacca +# aacagtacggtatgagcccgatttgtcatttgttgactggcttctttcaatgtcgaagggggttgactcgaaaattgatgaaccaacaaagagaacct +# taagtagggcagccagggtggagaacatttatgttgagaaggacggaaatctaagacacaacacagatccatatgctcttgaactccaaaataaggac +# gttaacaaccgttatcataactacttgaaggaaagattgcagtcggcaatagatcgtccaattgtcacactcgctagtgacccatcgttatccaggtc +# atatatagatccactagtctatgagatcagtagtggcacttacgaaaaatcctaa] +# protein sequence = [MIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASV +# TIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPP +# SQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDR +# PIVTLASDPSLSRSYIDPLVYEISSGTYEKS] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g24 +### +# start gene g25 +pf_alpha_strain65 AUGUSTUS gene 8788 10017 1 - . ID=g25 +pf_alpha_strain65 AUGUSTUS transcript 8788 10017 1 - . ID=g25.t1;Parent=g25 +pf_alpha_strain65 AUGUSTUS stop_codon 8788 8790 . - 0 Parent=g25.t1 +pf_alpha_strain65 AUGUSTUS CDS 8791 10017 1 - 0 ID=g25.t1.cds;Parent=g25.t1 +pf_alpha_strain65 AUGUSTUS start_codon 10015 10017 . - 0 Parent=g25.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacatacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctattatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggcgcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaattga] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g25 +### +# start gene g26 +pf_alpha_strain65 AUGUSTUS gene 10323 10844 1 + . ID=g26 +pf_alpha_strain65 AUGUSTUS transcript 10323 10844 1 + . ID=g26.t1;Parent=g26 +pf_alpha_strain65 AUGUSTUS start_codon 10323 10325 . + 0 Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS CDS 10323 10841 1 + 0 ID=g26.t1.cds;Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 10842 10844 . + 0 Parent=g26.t1 +# coding sequence = [atgaacagcataacccaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g26 +### +# start gene g27 +pf_alpha_strain65 AUGUSTUS gene 11081 12871 0.62 - . ID=g27 +pf_alpha_strain65 AUGUSTUS transcript 11081 12871 0.62 - . ID=g27.t1;Parent=g27 +pf_alpha_strain65 AUGUSTUS stop_codon 11081 11083 . - 0 Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS CDS 11084 12871 0.62 - 0 ID=g27.t1.cds;Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS start_codon 12869 12871 . - 0 Parent=g27.t1 +# coding sequence = [atgaagaaggcaagatccaccatgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggac +# agcacgctgatggctcggacgtcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcaccaccaggag +# acttcatccaaggacgagcagctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcag +# tcttccgtttggtggaaatgcgatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggt +# atgaccctactctttcaaggcatgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaag +# ggtgttgacaacggtaggttcagagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgt +# gtgggaccagattgaaggtgaggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctt +# tcaagacagggctcaatttgaacaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagaca +# ctaaatcatgttggaccagtcgatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagact +# gtcctgcgatataagttcacagaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcact +# caatggggggcttggttgcccaccatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtg +# ctcggtccaatccgctatggtgataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacc +# tgaggatggccgctgcttcatcgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccag +# cagtggctaagagccgccagaaagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaa +# cgttctctctctttgaggtcctctcacaagtcgacagcagaagaatttaatgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaag +# aaccaaaaagttcctcgatgaccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtg +# cccgtgttgatggtgtcgctgatatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgttcatcataagtggttgatgcca +# gagagaagaggattccctgttgcggccaaactttcaagtggtgaaggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgc +# agtgctacaggaagagaaagcaagactatctgctagagatggataa] +# protein sequence = [MKKARSTMSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLE +# SSHYKNEHKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAIS +# IPSLIDEKRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTN +# GKTNVHTFGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTP +# EINFFMRSSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVS +# FDESYAYLSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHIS +# LMTDLETMGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g27 +### +# start gene g28 +pf_alpha_strain65 AUGUSTUS gene 13565 14224 0.99 + . ID=g28 +pf_alpha_strain65 AUGUSTUS transcript 13565 14224 0.99 + . ID=g28.t1;Parent=g28 +pf_alpha_strain65 AUGUSTUS start_codon 13565 13567 . + 0 Parent=g28.t1 +pf_alpha_strain65 AUGUSTUS CDS 13565 14221 0.99 + 0 ID=g28.t1.cds;Parent=g28.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 14222 14224 . + 0 Parent=g28.t1 +# coding sequence = [atgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggac +# ctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaag +# gatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagttt +# ggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcgacgttacagagggtacaccattgc +# acagagtcgctgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttaaagtttgatggc +# tgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggt +# tgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNS +# RVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFII +# KSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g28 +### +# start gene g29 +pf_alpha_strain65 AUGUSTUS gene 14368 15765 1 - . ID=g29 +pf_alpha_strain65 AUGUSTUS transcript 14368 15765 1 - . ID=g29.t1;Parent=g29 +pf_alpha_strain65 AUGUSTUS stop_codon 14368 14370 . - 0 Parent=g29.t1 +pf_alpha_strain65 AUGUSTUS CDS 14371 15765 1 - 0 ID=g29.t1.cds;Parent=g29.t1 +pf_alpha_strain65 AUGUSTUS start_codon 15763 15765 . - 0 Parent=g29.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggcgacgctcgaggaagtggtagaggggaaggaagaagtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccttcaactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaggagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatttttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccatcttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRSNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDRSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g29 +### +# start gene g30 +pf_alpha_strain65 AUGUSTUS gene 15970 17838 0.55 + . ID=g30 +pf_alpha_strain65 AUGUSTUS transcript 15970 17838 0.55 + . ID=g30.t1;Parent=g30 +pf_alpha_strain65 AUGUSTUS start_codon 15970 15972 . + 0 Parent=g30.t1 +pf_alpha_strain65 AUGUSTUS CDS 15970 17835 0.55 + 0 ID=g30.t1.cds;Parent=g30.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 17836 17838 . + 0 Parent=g30.t1 +# coding sequence = [atgaattcccaacggcatacaccctatattcgagctgactcctccagtgtgctaccgaggacgctgggccgtcgaccgc +# ccggccctgtcatcccgaacctgggagctaagcaccatcacagctctctcaacaaaccccctcgatcatctcgacagcatgatgaacgagagtcttca +# tcacctcccagaccacacagctcctttgccccacccatcaactccaattcctcaatcacgtccgcctctagtcccaaaaaaaacgttaccgatgggcg +# ggaccccgccaacttgatctccacaacgtatgcctactgcggcaacaggaaacgactgcagaatccaagggaagtcgaaaataaaaagagactaagag +# agatggaagcaaaaaatgctcctggggaaccgccggtgcctctacagtctaaaaggtttcaacaggaagcgtcagttgtcaaggcgaatgatggggac +# acctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctagggagaaacgtgccccaat +# aatgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaactccattcgtgctccaagga +# ttcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagaggtcaaggaaatggatcaa +# caattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcgctgattaaaggtgtgaa +# atcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtcagggagtaagtcacctc +# accgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttcagtgggggacgctctaggc +# tcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaaggatgatgaaagtcataaagc +# tatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatcttgagatgatgaagcgca +# ggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatgagtttgaagaggttgac +# gagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacactaagcctcggtgagattgagcaaaa +# ggccaaccagcacatggactgcattccaaaaattctcgacggtcgtcttgactccacttacctacaaaaagcgaaagtattcatgcacgactctggag +# caacaattttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaaggggctattgccaatctg +# atcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattcacattacacattctcgc +# tccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagttgattatggtcaataca +# tcgctgatttggtcccttgtgaatga] +# protein sequence = [MNSQRHTPYIRADSSSVLPRTLGRRPPGPVIPNLGAKHHHSSLNKPPRSSRQHDERESSSPPRPHSSFAPPINSNSSI +# TSASSPKKNVTDGRDPANLISTTYAYCGNRKRLQNPREVENKKRLREMEAKNAPGEPPVPLQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNR +# KRNILSSREKRAPIMSSGEEEQEGSRHRIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRETKKGEDV +# APIPRKRALIKGVKSSAKKSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGPSSQELR +# SAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNE +# EFPGTLSLGEIEQKANQHMDCIPKILDGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVI +# QFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g30 +### +# start gene g31 +pf_alpha_strain65 AUGUSTUS gene 18296 21421 1 + . ID=g31 +pf_alpha_strain65 AUGUSTUS transcript 18296 21421 1 + . ID=g31.t1;Parent=g31 +pf_alpha_strain65 AUGUSTUS start_codon 18296 18298 . + 0 Parent=g31.t1 +pf_alpha_strain65 AUGUSTUS CDS 18296 21418 1 + 0 ID=g31.t1.cds;Parent=g31.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 21419 21421 . + 0 Parent=g31.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcagaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaccgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaga +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacctcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYETDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g31 +### +# +# ----- prediction on sequence number 4 (length = 22501, name = pf_alpha_tbrc4498) ----- +# +# Predicted genes for sequence number 4 on both strands +# start gene g32 +pf_alpha_tbrc4498 AUGUSTUS gene 943 1800 0.91 - . ID=g32 +pf_alpha_tbrc4498 AUGUSTUS transcript 943 1800 0.91 - . ID=g32.t1;Parent=g32 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 943 945 . - 0 Parent=g32.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 946 1800 0.91 - 0 ID=g32.t1.cds;Parent=g32.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 1798 1800 . - 0 Parent=g32.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaaccgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccactgcaagcaagaagaagtcgagtgcaaacttgagtgatgacgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgactctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAATASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g32 +### +# start gene g33 +pf_alpha_tbrc4498 AUGUSTUS gene 2147 3073 0.98 - . ID=g33 +pf_alpha_tbrc4498 AUGUSTUS transcript 2147 3073 0.98 - . ID=g33.t1;Parent=g33 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 2147 2149 . - 0 Parent=g33.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 2150 3073 0.98 - 0 ID=g33.t1.cds;Parent=g33.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 3071 3073 . - 0 Parent=g33.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccttcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttgtctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggcctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTFSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g33 +### +# start gene g34 +pf_alpha_tbrc4498 AUGUSTUS gene 3734 6130 0.93 + . ID=g34 +pf_alpha_tbrc4498 AUGUSTUS transcript 3734 6130 0.93 + . ID=g34.t1;Parent=g34 +pf_alpha_tbrc4498 AUGUSTUS start_codon 3734 3736 . + 0 Parent=g34.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 3734 6127 0.93 + 0 ID=g34.t1.cds;Parent=g34.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 6128 6130 . + 0 Parent=g34.t1 +# coding sequence = [atgttgtcccaccgccatcagaaagctgctctctccaaactgcaaagaagtgcttacgcacattacccaacaccacatt +# cgccaatatctctaacaccaaatagatcaatatcgtctcttccggcctcgaagatgcttccagctgtcaattacgttcgccaagcagatccagatgcg +# attgttcttctcggtcatggtgaatcttaccatgctttcggcagagtcgatactaatctgattaaaggaatggtatcagctggcaatgcaagcgagat +# actctaccgtgatgatggactgacacaatggagattcaagtcatctcgactcccgcataacacttcttggctggtgaaggagttgaacaggccagttg +# tcaccttggaatggaacgaatatgctactagtttcctacagccattgcgcaacgactcccattggcatttcctatcgagaaccattcctgatggccac +# tcagaccatgaattctctgttcattctgaatctcaaaacatcatagtaattggtatacaggggaagattctcaaggggatcaaaacaggtgaaaagtc +# acagagacgtgttggtatcacttgctttgacccaacaaacggatcgatgtctgttcagggcacttctcttgctgatttgagatcccatatcgtgagct +# ttcgtcctcgggctattgttctggactccagtctgattgggacataccgggacatcattggcacagctcttctaggttcaccagaagttaagtttacc +# aatatgcctcccgtgttgggctcgctacaactccttaatgatttctttgatgtctctggtgaaatttggaggaagtgtttcgatgattacgagctgta +# ctcactgaaggctgctgtggagttcattaagaagacacagaaatcaacgtctgcagtattccaactaccatcgagggaactgtcgtcacaatggaccg +# tgttattggacaaagaaactatcagttccttgaacctgggctccaccgggagtcactatatgaattcctcctggttacttcaaagatcaatcaagact +# gctactgttggtggttctcgaatgttgtacaagtctatcttgaggccaactaggatagtcagtgagattttgtttcgtcagagggcggttcaatccat +# cattaaaaggaaacatacacgtcagaagctgagctcagagctctcgagtttaccagacatcccattgctactacagaaactcaagatctatccggaat +# cagaagaccttgtcagtactatactacactccatcgacatcatctcgtcacttgctcccatcgcctccaagttttacatgaacggtcgccagcaagac +# gctgactttgatgatggtgacttgatttaccgcattccatctgcaaaacgtttggaggaactcagagacagggcccgttcccttctcaatgctagcga +# cacaatgcatggtgttgtttcctcgaatacaagcattgaatgcaatgatcggaggacacagaaagctactttggtgaatggtgttgattgtggaccta +# ataggaacacttctttttctcactctgtgaaaaatcagacaggtgatgtttctttggaaacctttatggtcagcgatgatgctgaattgaacactgag +# tcaccttctaatctcacaacagtgctagacacccgtactgtgagtgaacacacccagcctaaggggctaggagagattaccagctcatctttggacta +# ccgagatctacatgctacacaccgcatcgacaagttggccggtcagataagtgaattacaccctggccaccctctaccaagagccaaagtgattgcca +# actgctcggctgaaatattaagacataaggagctcattctactcttctcccgactcttgtcacgtttggacatgctgctgtcgtttgccaacgtggct +# atccaacatgacgacacaagacctatcgtggatcccagcagaaccaactttgagattcacttgaaggacaaagatttcaatgctgctgctgatccatt +# gttgatgtcaaactcatcatcagtattgtggactaagggagagagaaaaaccgtggcagagctttacgggctgtacgccagtatcatcttattggcgc +# actcaggtgcttatgttccagccaagtctgctaacattggtgttgtcagccacatgctttgtcaactccgctctacaaatcctttcctattacattca +# gcgcttgagaagtctatggatggccgtcgtatcatcatgtgcgacgctctgcctgacgatttcgaagactatggctcagccgatgacagattggaaag +# ctaccaagaaacactggcgactatgaatgtgggaactaaccgactgcttctaagatatcactga] +# protein sequence = [MLSHRHQKAALSKLQRSAYAHYPTPHSPISLTPNRSISSLPASKMLPAVNYVRQADPDAIVLLGHGESYHAFGRVDTN +# LIKGMVSAGNASEILYRDDGLTQWRFKSSRLPHNTSWLVKELNRPVVTLEWNEYATSFLQPLRNDSHWHFLSRTIPDGHSDHEFSVHSESQNIIVIGI +# QGKILKGIKTGEKSQRRVGITCFDPTNGSMSVQGTSLADLRSHIVSFRPRAIVLDSSLIGTYRDIIGTALLGSPEVKFTNMPPVLGSLQLLNDFFDVS +# GEIWRKCFDDYELYSLKAAVEFIKKTQKSTSAVFQLPSRELSSQWTVLLDKETISSLNLGSTGSHYMNSSWLLQRSIKTATVGGSRMLYKSILRPTRI +# VSEILFRQRAVQSIIKRKHTRQKLSSELSSLPDIPLLLQKLKIYPESEDLVSTILHSIDIISSLAPIASKFYMNGRQQDADFDDGDLIYRIPSAKRLE +# ELRDRARSLLNASDTMHGVVSSNTSIECNDRRTQKATLVNGVDCGPNRNTSFSHSVKNQTGDVSLETFMVSDDAELNTESPSNLTTVLDTRTVSEHTQ +# PKGLGEITSSSLDYRDLHATHRIDKLAGQISELHPGHPLPRAKVIANCSAEILRHKELILLFSRLLSRLDMLLSFANVAIQHDDTRPIVDPSRTNFEI +# HLKDKDFNAAADPLLMSNSSSVLWTKGERKTVAELYGLYASIILLAHSGAYVPAKSANIGVVSHMLCQLRSTNPFLLHSALEKSMDGRRIIMCDALPD +# DFEDYGSADDRLESYQETLATMNVGTNRLLLRYH] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g34 +### +# start gene g35 +pf_alpha_tbrc4498 AUGUSTUS gene 8041 8958 0.67 + . ID=g35 +pf_alpha_tbrc4498 AUGUSTUS transcript 8041 8958 0.67 + . ID=g35.t1;Parent=g35 +pf_alpha_tbrc4498 AUGUSTUS start_codon 8041 8043 . + 0 Parent=g35.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 8041 8955 0.67 + 0 ID=g35.t1.cds;Parent=g35.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 8956 8958 . + 0 Parent=g35.t1 +# coding sequence = [atgattgaagttcctctcggagcacttttggctcttagtgatccatacaccttctcttcacaaggtaaaatgaccggta +# tatcaaagattgtgaagaagcgaaacaaacaaggagttgaggttcttcgtttccgcggatgcccagcgtctaaagtgaggggcacaaggggtgcccaa +# ggactgcatcagtgtatggaaaggaacaattccatgaccagtggagaagcatccgtaacaatttttgctgctacgaagcttcctgatataccaagcat +# accgtatactttacagaagcagctggatgagctccgctctcagacaaccacatcagaccgcaggaagatcaaacgccttccccagctgcaagtgtcgg +# tgaacagagatgatacggttattaaaagggccaccaaaaccaaactaaatgggtttatggctttccgggcatttcattctcgtgaaatttctactatt +# gaacaccaaaggggtatttcgatgtcactggcgactgtgtgggcgagcgatcctccctcgcaacttgtctggcaaagattcgcagcagaatataacca +# aacagtacggtatgagcccgatttgtcatttgttgactggcttctttcaatgtcgaagggggttgactcgaaaattgatgaaccaacaaagagaacct +# taagtagggcagccagggtggagaacatttatgttgagaaggacggaaatctaagacacaacacagatccatatgctcttgaactccaaaataaggac +# gttaacaaccgttatcataactacttgaaggaaagattgcagtcggcaatagatcgtccaattgtcacactcgctagtgacccatcgttatccaggtc +# atatatagatccactagtctatgagatcagtagtggcacttacgaaaaatcctaa] +# protein sequence = [MIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASV +# TIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPP +# SQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDR +# PIVTLASDPSLSRSYIDPLVYEISSGTYEKS] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g35 +### +# start gene g36 +pf_alpha_tbrc4498 AUGUSTUS gene 9028 10257 1 - . ID=g36 +pf_alpha_tbrc4498 AUGUSTUS transcript 9028 10257 1 - . ID=g36.t1;Parent=g36 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 9028 9030 . - 0 Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 9031 10257 1 - 0 ID=g36.t1.cds;Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 10255 10257 . - 0 Parent=g36.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# ttggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctattatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggcgcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaattga] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g36 +### +# start gene g37 +pf_alpha_tbrc4498 AUGUSTUS gene 10563 11084 1 + . ID=g37 +pf_alpha_tbrc4498 AUGUSTUS transcript 10563 11084 1 + . ID=g37.t1;Parent=g37 +pf_alpha_tbrc4498 AUGUSTUS start_codon 10563 10565 . + 0 Parent=g37.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 10563 11081 1 + 0 ID=g37.t1.cds;Parent=g37.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 11082 11084 . + 0 Parent=g37.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g37 +### +# start gene g38 +pf_alpha_tbrc4498 AUGUSTUS gene 11321 13111 0.55 - . ID=g38 +pf_alpha_tbrc4498 AUGUSTUS transcript 11321 13111 0.55 - . ID=g38.t1;Parent=g38 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 11321 11323 . - 0 Parent=g38.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 11324 13111 0.55 - 0 ID=g38.t1.cds;Parent=g38.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 13109 13111 . - 0 Parent=g38.t1 +# coding sequence = [atgaagaaggcaagatccaccatgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggac +# agcacgcggatggctcggacgtcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcaccaccaggag +# acttcatccaaggacgagcagctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcag +# tcttccgtttggtggaaatgcgatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggt +# atgaccctactctttcaaggcatgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaag +# ggtgttgacaacggtaggttcagagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgt +# gtgggaccagattgaaggtgaggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctt +# tcaagacagggctcaatttgaacaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagaca +# ctaaatcatgttggaccagtcgatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagact +# gtcctgcgatataagttcacagaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcact +# caatggggggcttggttgcccaccatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtg +# ctcggtccaatccgctatggtgataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacc +# tgaggatggccgctgcttcatcgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccag +# cagtggctaagagccgccagaaagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaa +# cgttctctctctttgaggtcctctcacaagtcgacagcagaagaatttaacgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaag +# aaccaaaaagttcctcgatgaccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtg +# cccgtgttgatggtgtcgctgatatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgttcatcataagtggttgatgcca +# gagagaagaggattccctgttgcggccaaactttcaagtggtgaaggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgc +# agtgctacaggaagagaaagcaagactatctgctagagatggataa] +# protein sequence = [MKKARSTMSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLE +# SSHYKNEHKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAIS +# IPSLIDEKRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTN +# GKTNVHTFGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTP +# EINFFMRSSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVS +# FDESYAYLSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHIS +# LMTDLETMGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g38 +### +# start gene g39 +pf_alpha_tbrc4498 AUGUSTUS gene 13805 14464 0.99 + . ID=g39 +pf_alpha_tbrc4498 AUGUSTUS transcript 13805 14464 0.99 + . ID=g39.t1;Parent=g39 +pf_alpha_tbrc4498 AUGUSTUS start_codon 13805 13807 . + 0 Parent=g39.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 13805 14461 0.99 + 0 ID=g39.t1.cds;Parent=g39.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 14462 14464 . + 0 Parent=g39.t1 +# coding sequence = [atgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggac +# ctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaag +# gatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagttt +# ggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaaactgccaagtttcgacgttacagagggtacaccattgc +# acagagtcgctgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttaaagtttgatggc +# tgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggt +# tgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNS +# RVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFII +# KSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g39 +### +# start gene g40 +pf_alpha_tbrc4498 AUGUSTUS gene 14608 16005 1 - . ID=g40 +pf_alpha_tbrc4498 AUGUSTUS transcript 14608 16005 1 - . ID=g40.t1;Parent=g40 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 14608 14610 . - 0 Parent=g40.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 14611 16005 1 - 0 ID=g40.t1.cds;Parent=g40.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 16003 16005 . - 0 Parent=g40.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggtgacgctcgaggaagtggtagaggggatggaagaggtaacagaggtgatagaggtgacagaaacagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtaagtgatcgtgctgatgctagtcgtcacacatcgagaagagacgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatatttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccaccttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGDGRGNRGDRGDRNRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADASRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g40 +### +# start gene g41 +pf_alpha_tbrc4498 AUGUSTUS gene 16852 18078 0.66 + . ID=g41 +pf_alpha_tbrc4498 AUGUSTUS transcript 16852 18078 0.66 + . ID=g41.t1;Parent=g41 +pf_alpha_tbrc4498 AUGUSTUS start_codon 16852 16854 . + 0 Parent=g41.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 16852 18075 0.66 + 0 ID=g41.t1.cds;Parent=g41.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 18076 18078 . + 0 Parent=g41.t1 +# coding sequence = [atgaactccattcgtgctccaaggattcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtc +# tacaacataaacgtgaggatacagaggtcaaggaaatggatcaacaattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagat +# gtcgctccaataccaaggaagagggcgctgattaaaggtgtgaaatcttctgctaagaaaagcacaggatcgacgagtcaaccattgggtgatattag +# gggcatgctgtctggaaagaaaaagtcagggagtaagtcacctcaccgccgtggcaccgaagacttgggtactgaagatccaggtggtgagacacaca +# agcaccgggaccgtcatgaaggttcagtgggggacgctctaggctcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaagaattg +# cgttccgccaaagagcttctcaaggatgatgaaagtcataaagctatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacg +# attcaaacccgatggtgatgactatcttgagatgatgaagcgcaggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatc +# aggatatcagtgttggtctacaaaatgagtttgaagaggttgacgagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaat +# gaggagttcccgggaacattaagcctcggtgagattgagcaaaaggccaaccagcacatggactgcattccaaaaattctcaacggtcgtcttgactc +# cacttacctacaaaaagcgaaagtattcatgcacgactctggagcaacaatcttgtccaatgcagatctccaaagacctagtttcaagcagttcttta +# gtggattttatggtttgaagaggcaaggggctattgccaatctgatcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggtt +# atccaattttggtccatcgatacattcacattacacattctcgctccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatga +# ggctatcagagtgttggatagaacagttgattatggtcaatacatcgctgatttggtcccttgtgaatga] +# protein sequence = [MNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRETKKGEDVAPIPRKRALIKGVKSSAK +# KSTGSTSQPLGDIRGMLSGKKKSGSKSPHRRGTEDLGTEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGPSSQELRSAKELLKDDESHKAMKSA +# IDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNEEFPGTLSLGEIEQKANQH +# MDCIPKILNGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVIQFWSIDTFTLHILAPEVA +# ARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 5 +# RM: 5 +# end gene g41 +### +# start gene g42 +pf_alpha_tbrc4498 AUGUSTUS gene 18536 21661 1 + . ID=g42 +pf_alpha_tbrc4498 AUGUSTUS transcript 18536 21661 1 + . ID=g42.t1;Parent=g42 +pf_alpha_tbrc4498 AUGUSTUS start_codon 18536 18538 . + 0 Parent=g42.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 18536 21658 1 + 0 ID=g42.t1.cds;Parent=g42.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 21659 21661 . + 0 Parent=g42.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgaaaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g42 +### +# command line: +# augustus --species=candida_guilliermondii --genemodel=complete --strand=both --protein=on --codingseq=on --gff3=on windows.fa diff --git a/results/2026-10-30_petasospora/data/aug_classified.tsv b/results/2026-10-30_petasospora/data/aug_classified.tsv new file mode 100644 index 00000000..a2b52c77 --- /dev/null +++ b/results/2026-10-30_petasospora/data/aug_classified.tsv @@ -0,0 +1,171 @@ +species window gene gstart gend strand cds aa best_ref bits pfam MATa2_profile +candida_guilliermondii pf_a_cp048715 g1.t1 627248 628102 - 627248-628102 285 [] +candida_guilliermondii pf_a_cp048715 g2.t1 628452 629375 - 628452-629375 308 dic1 374 [] +candida_guilliermondii pf_a_cp048715 g3.t1 629853 630242 - 629853-630242 130 [] +candida_guilliermondii pf_a_cp048715 g4.t1 632244 633470 - 632244-633470 409 [] +candida_guilliermondii pf_a_cp048715 g5.t1 633775 634293 + 633775-634293 173 [] +candida_guilliermondii pf_a_cp048715 g6.t1 634537 636324 - 634537-636324 596 [] +candida_guilliermondii pf_a_cp048715 g7.t1 637015 637671 + 637015-637671 219 [] +candida_guilliermondii pf_a_cp048715 g8.t1 637821 639215 - 637821-639215 465 [] +candida_guilliermondii pf_a_cp048715 g9.t1 639420 641285 + 639420-641285 622 [] +candida_guilliermondii pf_a_cp048715 g10.t1 641746 644868 + 641746-644868 1041 sla2 1233 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +candida_guilliermondii pf_a_jcm3601 g11.t1 639946 640800 - 639946-640800 285 [] +candida_guilliermondii pf_a_jcm3601 g12.t1 641150 642073 - 641150-642073 308 dic1 378 [] +candida_guilliermondii pf_a_jcm3601 g13.t1 642551 642940 - 642551-642940 130 [] +candida_guilliermondii pf_a_jcm3601 g14.t1 644939 646165 - 644939-646165 409 [] +candida_guilliermondii pf_a_jcm3601 g15.t1 646471 646989 + 646471-646989 173 [] +candida_guilliermondii pf_a_jcm3601 g16.t1 647233 649020 - 647233-649020 596 [] +candida_guilliermondii pf_a_jcm3601 g17.t1 649710 650366 + 649710-650366 219 [] +candida_guilliermondii pf_a_jcm3601 g18.t1 650516 651910 - 650516-651910 465 [] +candida_guilliermondii pf_a_jcm3601 g19.t1 652757 653980 + 652757-653980 408 [] +candida_guilliermondii pf_a_jcm3601 g20.t1 654441 657563 + 654441-657563 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 201.6)] +candida_guilliermondii pf_alpha_strain65 g21.t1 610206 611060 - 610206-611060 285 [] +candida_guilliermondii pf_alpha_strain65 g22.t1 611410 612333 - 611410-612333 308 dic1 376 [] +candida_guilliermondii pf_alpha_strain65 g23.t1 612994 615387 + 612994-615387 798 [] +candida_guilliermondii pf_alpha_strain65 g24.t1 617300 618214 + 617300-618214 305 ONH67106.1 408 [('MATalpha_HMGbox', 36.4)] +candida_guilliermondii pf_alpha_strain65 g25.t1 618290 619516 - 618290-619516 409 [] +candida_guilliermondii pf_alpha_strain65 g26.t1 619822 620340 + 619822-620340 173 [] +candida_guilliermondii pf_alpha_strain65 g27.t1 620583 622370 - 620583-622370 596 [] +candida_guilliermondii pf_alpha_strain65 g28.t1 623064 623720 + 623064-623720 219 [] +candida_guilliermondii pf_alpha_strain65 g29.t1 623870 625264 - 623870-625264 465 [] +candida_guilliermondii pf_alpha_strain65 g30.t1 625469 627334 + 625469-627334 622 [] +candida_guilliermondii pf_alpha_strain65 g31.t1 627795 630917 + 627795-630917 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +candida_guilliermondii pf_alpha_tbrc4498 g32.t1 630445 631299 - 630445-631299 285 [] +candida_guilliermondii pf_alpha_tbrc4498 g33.t1 631649 632572 - 631649-632572 308 dic1 377 [] +candida_guilliermondii pf_alpha_tbrc4498 g34.t1 633233 635626 + 633233-635626 798 [] +candida_guilliermondii pf_alpha_tbrc4498 g35.t1 637540 638454 + 637540-638454 305 ONH67106.1 408 [('MATalpha_HMGbox', 36.4)] +candida_guilliermondii pf_alpha_tbrc4498 g36.t1 638530 639756 - 638530-639756 409 [] +candida_guilliermondii pf_alpha_tbrc4498 g37.t1 640062 640580 + 640062-640580 173 [] +candida_guilliermondii pf_alpha_tbrc4498 g38.t1 640823 642610 - 640823-642610 596 [] +candida_guilliermondii pf_alpha_tbrc4498 g39.t1 643304 643960 + 643304-643960 219 [] +candida_guilliermondii pf_alpha_tbrc4498 g40.t1 644110 645504 - 644110-645504 465 [] +candida_guilliermondii pf_alpha_tbrc4498 g41.t1 646351 647574 + 646351-647574 408 [] +candida_guilliermondii pf_alpha_tbrc4498 g42.t1 648035 651157 + 648035-651157 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +kluyveromyces_lactis pf_a_cp048715 g1.t1 627248 628102 - 627248-628102 285 [] +kluyveromyces_lactis pf_a_cp048715 g2.t1 628452 629375 - 628452-629375 308 dic1 374 [] +kluyveromyces_lactis pf_a_cp048715 g3.t1 629853 630242 - 629853-630242 130 [] +kluyveromyces_lactis pf_a_cp048715 g4.t1 631437 632133 + 631437-631737;631865-632133 190 60523_cbs-4732_MATog_MATa-MATalpha_MATa1 65 [('Homeodomain', 64.0), ('Homeobox_KN', 29.7)] +kluyveromyces_lactis pf_a_cp048715 g5.t1 632244 633470 - 632244-633470 409 [] +kluyveromyces_lactis pf_a_cp048715 g6.t1 633775 634293 + 633775-634293 173 [] +kluyveromyces_lactis pf_a_cp048715 g7.t1 634537 636303 - 634537-636303 589 [] +kluyveromyces_lactis pf_a_cp048715 g8.t1 636359 637671 + 636359-636527;636956-637671 295 [] +kluyveromyces_lactis pf_a_cp048715 g9.t1 637821 639215 - 637821-639215 465 [] +kluyveromyces_lactis pf_a_cp048715 g10.t1 639420 641285 + 639420-641285 622 [] +kluyveromyces_lactis pf_a_cp048715 g11.t1 641746 644868 + 641746-644868 1041 sla2 1233 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +kluyveromyces_lactis pf_a_jcm3601 g12.t1 639946 640800 - 639946-640800 285 [] +kluyveromyces_lactis pf_a_jcm3601 g13.t1 641150 642073 - 641150-642073 308 dic1 378 [] +kluyveromyces_lactis pf_a_jcm3601 g14.t1 642551 642940 - 642551-642940 130 [] +kluyveromyces_lactis pf_a_jcm3601 g15.t1 644135 644831 + 644135-644435;644563-644831 190 MATA1 65 [('Homeodomain', 64.0), ('Homeobox_KN', 29.7)] +kluyveromyces_lactis pf_a_jcm3601 g16.t1 644939 646165 - 644939-646165 409 [] +kluyveromyces_lactis pf_a_jcm3601 g17.t1 646471 646989 + 646471-646989 173 [] +kluyveromyces_lactis pf_a_jcm3601 g18.t1 647233 648999 - 647233-648999 589 [] +kluyveromyces_lactis pf_a_jcm3601 g19.t1 649710 650366 + 649710-650366 219 [] +kluyveromyces_lactis pf_a_jcm3601 g20.t1 650516 651910 - 650516-651910 465 [] +kluyveromyces_lactis pf_a_jcm3601 g21.t1 652490 653980 + 652490-653980 497 [] +kluyveromyces_lactis pf_a_jcm3601 g22.t1 654441 657563 + 654441-657563 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 201.6)] +kluyveromyces_lactis pf_alpha_strain65 g23.t1 610206 611060 - 610206-611060 285 [] +kluyveromyces_lactis pf_alpha_strain65 g24.t1 611410 612333 - 611410-612333 308 dic1 376 [] +kluyveromyces_lactis pf_alpha_strain65 g25.t1 612994 615387 + 612994-615387 798 [] +kluyveromyces_lactis pf_alpha_strain65 g26.t1 617300 618214 + 617300-618214 305 ONH67106.1 408 [('MATalpha_HMGbox', 36.4)] +kluyveromyces_lactis pf_alpha_strain65 g27.t1 618290 619516 - 618290-619516 409 [] +kluyveromyces_lactis pf_alpha_strain65 g28.t1 619822 620340 + 619822-620340 173 [] +kluyveromyces_lactis pf_alpha_strain65 g29.t1 620583 622349 - 620583-622349 589 [] +kluyveromyces_lactis pf_alpha_strain65 g30.t1 622809 623720 + 622809-622830;623005-623720 246 [] +kluyveromyces_lactis pf_alpha_strain65 g31.t1 623870 625264 - 623870-625264 465 [] +kluyveromyces_lactis pf_alpha_strain65 g32.t1 625844 627334 + 625844-627334 497 [] +kluyveromyces_lactis pf_alpha_strain65 g33.t1 627795 630917 + 627795-630917 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +kluyveromyces_lactis pf_alpha_tbrc4498 g34.t1 630445 631299 - 630445-631299 285 [] +kluyveromyces_lactis pf_alpha_tbrc4498 g35.t1 631649 632572 - 631649-632572 308 dic1 377 [] +kluyveromyces_lactis pf_alpha_tbrc4498 g36.t1 633233 635626 + 633233-635626 798 [] +kluyveromyces_lactis pf_alpha_tbrc4498 g37.t1 637540 638454 + 637540-638454 305 ONH67106.1 408 [('MATalpha_HMGbox', 36.4)] +kluyveromyces_lactis pf_alpha_tbrc4498 g38.t1 638530 639756 - 638530-639756 409 [] +kluyveromyces_lactis pf_alpha_tbrc4498 g39.t1 640062 640580 + 640062-640580 173 [] +kluyveromyces_lactis pf_alpha_tbrc4498 g40.t1 640823 642589 - 640823-642589 589 [] +kluyveromyces_lactis pf_alpha_tbrc4498 g41.t1 643049 643960 + 643049-643070;643245-643960 246 [] +kluyveromyces_lactis pf_alpha_tbrc4498 g42.t1 644110 645504 - 644110-645504 465 [] +kluyveromyces_lactis pf_alpha_tbrc4498 g43.t1 646084 647574 + 646084-647574 497 [] +kluyveromyces_lactis pf_alpha_tbrc4498 g44.t1 648035 651157 + 648035-651157 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +pichia_stipitis pf_a_cp048715 g1.t1 627248 628102 - 627248-628102 285 [] +pichia_stipitis pf_a_cp048715 g2.t1 628452 629375 - 628452-629375 308 dic1 374 [] +pichia_stipitis pf_a_cp048715 g3.t1 629853 630863 - 629853-630419;630492-630863 313 MATA 50 [('HMG_box', 13.5)] 76.8 +pichia_stipitis pf_a_cp048715 g4.t1 632244 633470 - 632244-633470 409 [] +pichia_stipitis pf_a_cp048715 g5.t1 633775 634293 + 633775-634293 173 [] +pichia_stipitis pf_a_cp048715 g6.t1 634537 636303 - 634537-636303 589 [] +pichia_stipitis pf_a_cp048715 g7.t1 636441 637671 + 636441-636534;636890-637671 292 [] +pichia_stipitis pf_a_cp048715 g8.t1 637821 639215 - 637821-639215 465 [] +pichia_stipitis pf_a_cp048715 g9.t1 639990 641285 + 639990-641285 432 [] +pichia_stipitis pf_a_cp048715 g10.t1 641746 644868 + 641746-644868 1041 sla2 1233 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +pichia_stipitis pf_a_jcm3601 g11.t1 639946 642073 - 639946-640592;641155-642073 522 dic1 373 [] +pichia_stipitis pf_a_jcm3601 g12.t1 642551 643561 - 642551-643117;643190-643561 313 MATA 50 [('HMG_box', 13.5)] 76.5 +pichia_stipitis pf_a_jcm3601 g13.t1 644939 646165 - 644939-646165 409 [] +pichia_stipitis pf_a_jcm3601 g14.t1 646471 646989 + 646471-646989 173 [] +pichia_stipitis pf_a_jcm3601 g15.t1 647233 648999 - 647233-648999 589 [] +pichia_stipitis pf_a_jcm3601 g16.t1 649455 650366 + 649455-649476;649591-650366 266 [] +pichia_stipitis pf_a_jcm3601 g17.t1 650516 651910 - 650516-651910 465 [] +pichia_stipitis pf_a_jcm3601 g18.t1 652685 653980 + 652685-653980 432 [] +pichia_stipitis pf_a_jcm3601 g19.t1 654441 657563 + 654441-657563 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 201.6)] +pichia_stipitis pf_alpha_strain65 g20.t1 610206 611060 - 610206-611060 285 [] +pichia_stipitis pf_alpha_strain65 g21.t1 611410 612333 - 611410-612333 308 dic1 376 [] +pichia_stipitis pf_alpha_strain65 g22.t1 612994 615387 + 612994-615387 798 [] +pichia_stipitis pf_alpha_strain65 g23.t1 618290 619516 - 618290-619516 409 [] +pichia_stipitis pf_alpha_strain65 g24.t1 619822 620340 + 619822-620340 173 [] +pichia_stipitis pf_alpha_strain65 g25.t1 620583 622349 - 620583-622349 589 [] +pichia_stipitis pf_alpha_strain65 g26.t1 622487 623720 + 622487-622580;622945-623720 290 [] +pichia_stipitis pf_alpha_strain65 g27.t1 623870 625264 - 623870-624856;624968-625264 428 [] +pichia_stipitis pf_alpha_strain65 g28.t1 626039 627334 + 626039-627334 432 [] +pichia_stipitis pf_alpha_strain65 g29.t1 627795 630917 + 627795-630917 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +pichia_stipitis pf_alpha_tbrc4498 g30.t1 630445 631299 - 630445-631299 285 [] +pichia_stipitis pf_alpha_tbrc4498 g31.t1 631649 632572 - 631649-632572 308 dic1 377 [] +pichia_stipitis pf_alpha_tbrc4498 g32.t1 633233 635626 + 633233-635626 798 [] +pichia_stipitis pf_alpha_tbrc4498 g33.t1 638530 639756 - 638530-639756 409 [] +pichia_stipitis pf_alpha_tbrc4498 g34.t1 640062 640580 + 640062-640580 173 [] +pichia_stipitis pf_alpha_tbrc4498 g35.t1 640823 642589 - 640823-642589 589 [] +pichia_stipitis pf_alpha_tbrc4498 g36.t1 642727 643960 + 642727-642820;643185-643960 290 [] +pichia_stipitis pf_alpha_tbrc4498 g37.t1 644110 645504 - 644110-645504 465 [] +pichia_stipitis pf_alpha_tbrc4498 g38.t1 646279 647574 + 646279-647574 432 [] +pichia_stipitis pf_alpha_tbrc4498 g39.t1 648035 651157 + 648035-651157 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g1.t1 627248 628102 - 627248-628102 285 [] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g2.t1 628452 629375 - 628452-629375 308 dic1 374 [] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g3.t1 629853 630242 - 629853-630242 130 [] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g4.t1 630435 630863 - 630435-630863 143 MTLA2 25 [] 35.4 +saccharomyces_cerevisiae_S288C pf_a_cp048715 g5.t1 631437 632133 + 631437-631737;631835-632133 200 460519_cbs-7435_MATkp_MATa_MATa1 66 [('Homeodomain', 63.8), ('Homeobox_KN', 29.5)] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g6.t1 632244 633470 - 632244-633470 409 [] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g7.t1 633775 634293 + 633775-634293 173 [] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g8.t1 634537 636303 - 634537-636303 589 [] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g9.t1 637015 637671 + 637015-637671 219 [] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g10.t1 637821 639215 - 637821-639215 465 [] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g11.t1 639420 641285 + 639420-641285 622 [] +saccharomyces_cerevisiae_S288C pf_a_cp048715 g12.t1 641746 644868 + 641746-644868 1041 sla2 1233 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g13.t1 639946 640800 - 639946-640800 285 [] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g14.t1 641150 642073 - 641150-642073 308 dic1 378 [] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g15.t1 642551 643561 - 642551-643117;643190-643561 313 MATA 50 [('HMG_box', 13.5)] 76.5 +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g16.t1 644135 644831 + 644135-644435;644533-644831 200 460519_cbs-7435_MATkp_MATa_MATa1 66 [('Homeodomain', 63.8), ('Homeobox_KN', 29.6)] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g17.t1 644939 646165 - 644939-646165 409 [] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g18.t1 646471 646989 + 646471-646989 173 [] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g19.t1 647233 648999 - 647233-648999 589 [] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g20.t1 649710 650366 + 649710-650366 219 [] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g21.t1 650516 651910 - 650516-651910 465 [] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g22.t1 652115 653980 + 652115-653980 622 [] +saccharomyces_cerevisiae_S288C pf_a_jcm3601 g23.t1 654441 657563 + 654441-657563 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 201.6)] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g24.t1 610206 611060 - 610206-611060 285 [] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g25.t1 611410 612333 - 611410-612333 308 dic1 376 [] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g26.t1 612994 615387 + 612994-615387 798 [] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g27.t1 617300 618214 + 617300-618214 305 ONH67106.1 408 [('MATalpha_HMGbox', 36.4)] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g28.t1 618290 619516 - 618290-619516 409 [] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g29.t1 619822 620340 + 619822-620340 173 [] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g30.t1 620583 622349 - 620583-622349 589 [] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g31.t1 623064 623720 + 623064-623720 219 [] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g32.t1 623870 625264 - 623870-625264 465 [] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g33.t1 625469 627334 + 625469-627334 622 [] +saccharomyces_cerevisiae_S288C pf_alpha_strain65 g34.t1 627795 630917 + 627795-630917 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 202.1)] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g35.t1 630445 631299 - 630445-631299 285 [] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g36.t1 631649 632572 - 631649-632572 308 dic1 377 [] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g37.t1 633233 635626 + 633233-635626 798 [] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g38.t1 637540 638454 + 637540-638454 305 ONH67106.1 408 [('MATalpha_HMGbox', 36.4)] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g39.t1 638530 639756 - 638530-639756 409 [] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g40.t1 640062 640580 + 640062-640580 173 [] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g41.t1 640823 642589 - 640823-642589 589 [] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g42.t1 643304 643960 + 643304-643960 219 [] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g43.t1 644110 645504 - 644110-645504 465 [] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g44.t1 645709 647574 + 645709-647574 622 [] +saccharomyces_cerevisiae_S288C pf_alpha_tbrc4498 g45.t1 648035 651157 + 648035-651157 1041 sla2 1231 [('ANTH', 251.0), ('I_LWEQ', 202.1)] diff --git a/results/2026-10-30_petasospora/data/aug_kluyveromyces_lactis.gff3 b/results/2026-10-30_petasospora/data/aug_kluyveromyces_lactis.gff3 new file mode 100644 index 00000000..4c72117b --- /dev/null +++ b/results/2026-10-30_petasospora/data/aug_kluyveromyces_lactis.gff3 @@ -0,0 +1,1634 @@ +##gff-version 3 +# This output was generated with AUGUSTUS (version 3.5.0). +# AUGUSTUS is a gene prediction tool written by M. Stanke (mario.stanke@uni-greifswald.de), +# O. Keller, S. König, L. Gerischer, L. Romoth, Katharina Hoff, Henry Mehlan and Daniel Honsel. +# Please cite: Mario Stanke, Mark Diekhans, Robert Baertsch, David Haussler (2008), +# Using native and syntenically mapped cDNA alignments to improve de novo gene finding +# Bioinformatics 24: 637-644, doi 10.1093/bioinformatics/btn013 +# No extrinsic information on sequences given. +# Sources of extrinsic information: M RM +# Initializing the parameters using config directory /opt/linux/centos/8.x/x86_64/pkgs/augustus/3.5.0/env/config/ ... +# kluyveromyces_lactis version. Using default transition matrix. +# Looks like windows.fa is in fasta format. +# We have hints for 0 sequences and for 0 of the sequences in the input set. +# +# ----- prediction on sequence number 1 (length = 19001, name = pf_a_cp048715) ----- +# +# Predicted genes for sequence number 1 on both strands +# start gene g1 +pf_a_cp048715 AUGUSTUS gene 746 1603 0.74 - . ID=g1 +pf_a_cp048715 AUGUSTUS transcript 746 1603 0.74 - . ID=g1.t1;Parent=g1 +pf_a_cp048715 AUGUSTUS stop_codon 746 748 . - 0 Parent=g1.t1 +pf_a_cp048715 AUGUSTUS CDS 749 1603 0.74 - 0 ID=g1.t1.cds;Parent=g1.t1 +pf_a_cp048715 AUGUSTUS start_codon 1601 1603 . - 0 Parent=g1.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagaccagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcggaatacattagggcacaggatttcaagtatatcgagacgatttcagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# agaagagattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgatgaagatgac +# accagtgaagacacagagcgactcgtttctgacgctgctgatattatctggccaaagtcctatgattctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgacctcgctcgactattcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEDQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETISDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVKKRFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSDAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g1 +### +# start gene g2 +pf_a_cp048715 AUGUSTUS gene 1950 2876 0.87 - . ID=g2 +pf_a_cp048715 AUGUSTUS transcript 1950 2876 0.87 - . ID=g2.t1;Parent=g2 +pf_a_cp048715 AUGUSTUS stop_codon 1950 1952 . - 0 Parent=g2.t1 +pf_a_cp048715 AUGUSTUS CDS 1953 2876 0.87 - 0 ID=g2.t1.cds;Parent=g2.t1 +pf_a_cp048715 AUGUSTUS start_codon 2874 2876 . - 0 Parent=g2.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# cagcacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccagatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttgtctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggagtgctcatgacctcgtcacaggttgtcacctatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgagcaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaatactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVTARQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILSTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g2 +### +# start gene g3 +pf_a_cp048715 AUGUSTUS gene 3351 3743 0.58 - . ID=g3 +pf_a_cp048715 AUGUSTUS transcript 3351 3743 0.58 - . ID=g3.t1;Parent=g3 +pf_a_cp048715 AUGUSTUS stop_codon 3351 3353 . - 0 Parent=g3.t1 +pf_a_cp048715 AUGUSTUS CDS 3354 3743 0.58 - 0 ID=g3.t1.cds;Parent=g3.t1 +pf_a_cp048715 AUGUSTUS start_codon 3741 3743 . - 0 Parent=g3.t1 +# coding sequence = [atgtcagttatctttttgaacgatccggaaaactcaatcaagcagagtaagcaaaagaccgaaaaagcttcagctagtg +# atatcaaagatatgagcagttatgtcaagctttgcactagtgtttatgaggaagacgactgcaaacggaggggggtcgatgctctccacgattcagat +# gactacaaagaccatgtgcttgtattaacgcttaacgagcctaatatgaaagctccggttgactctgctagtacaaacgccaggacaacatataaggt +# aacaaagcccacaaaaaagaagaagacgccgcctcatttggagcttgtattcagtaaattcaggtcccctttgtatggtgaagccgaggatatttgca +# tcaacagttcccctatgtga] +# protein sequence = [MSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAP +# VDSASTNARTTYKVTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g3 +### +# start gene g4 +pf_a_cp048715 AUGUSTUS gene 4938 5637 0.86 + . ID=g4 +pf_a_cp048715 AUGUSTUS transcript 4938 5637 0.86 + . ID=g4.t1;Parent=g4 +pf_a_cp048715 AUGUSTUS start_codon 4938 4940 . + 0 Parent=g4.t1 +pf_a_cp048715 AUGUSTUS intron 5239 5365 0.86 + . Parent=g4.t1 +pf_a_cp048715 AUGUSTUS CDS 4938 5238 0.86 + 0 ID=g4.t1.cds;Parent=g4.t1 +pf_a_cp048715 AUGUSTUS CDS 5366 5634 0.88 + 2 ID=g4.t1.cds;Parent=g4.t1 +pf_a_cp048715 AUGUSTUS stop_codon 5635 5637 . + 0 Parent=g4.t1 +# coding sequence = [atggccacgagggatcaaatagcgtataaggaggcaagcgagaagcttgatgcccttatcgagttttcatctaacttct +# cgactaacccaacgaaaccgagatatgacaaaataatgctggaagatccgtttgagaaggacagtttactgagcaaggttccggcctatgcgagagca +# gcattggaaactctgatctcaaacttcgaactatccctcggacgtgttatggtaggacaatctctttcccataaatctaaccaatcggtcataaaaac +# taacaaaccattaggaccacgactcacatcgtacgaagtccagtctaccactatcgaagtaacgggatcgacttctagccctgtctttccaggtggag +# gtcgcaagcgaaatgcgcttccagcaaagaagcgaacatcaattacccctgaatccaaggagtttttggagagagtgtttaaggctaagaggactcca +# aacagtacggagaggaaagttatagcagaaaagtgtggactcacgccactgcaggttagggtatggttcacaaacaagagaatgaggaacaaggaagg +# ctga] +# protein sequence = [MATRDQIAYKEASEKLDALIEFSSNFSTNPTKPRYDKIMLEDPFEKDSLLSKVPAYARAALETLISNFELSLGRVMVG +# QSLSHKSNQSVIKTNKPLGPRLTSYEVQSTTIEVTGSTSSPVFPGGGRKRNALPAKKRTSITPESKEFLERVFKAKRTPNSTERKVIAEKCGLTPLQV +# RVWFTNKRMRNKEG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 2 +# RM: 2 +# incompatible hint groups: 0 +# end gene g4 +### +# start gene g5 +pf_a_cp048715 AUGUSTUS gene 5742 6971 1 - . ID=g5 +pf_a_cp048715 AUGUSTUS transcript 5742 6971 1 - . ID=g5.t1;Parent=g5 +pf_a_cp048715 AUGUSTUS stop_codon 5742 5744 . - 0 Parent=g5.t1 +pf_a_cp048715 AUGUSTUS CDS 5745 6971 1 - 0 ID=g5.t1.cds;Parent=g5.t1 +pf_a_cp048715 AUGUSTUS start_codon 6969 6971 . - 0 Parent=g5.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacgagacaagagctcgagaagacggtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcacaggaatcaccgacgactcccagacatccaaacta +# cagctcatctactatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggctcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaactag] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLTGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g5 +### +# start gene g6 +pf_a_cp048715 AUGUSTUS gene 7276 7797 1 + . ID=g6 +pf_a_cp048715 AUGUSTUS transcript 7276 7797 1 + . ID=g6.t1;Parent=g6 +pf_a_cp048715 AUGUSTUS start_codon 7276 7278 . + 0 Parent=g6.t1 +pf_a_cp048715 AUGUSTUS CDS 7276 7794 1 + 0 ID=g6.t1.cds;Parent=g6.t1 +pf_a_cp048715 AUGUSTUS stop_codon 7795 7797 . + 0 Parent=g6.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g6 +### +# start gene g7 +pf_a_cp048715 AUGUSTUS gene 8035 9804 0.88 - . ID=g7 +pf_a_cp048715 AUGUSTUS transcript 8035 9804 0.88 - . ID=g7.t1;Parent=g7 +pf_a_cp048715 AUGUSTUS stop_codon 8035 8037 . - 0 Parent=g7.t1 +pf_a_cp048715 AUGUSTUS CDS 8038 9804 0.88 - 0 ID=g7.t1.cds;Parent=g7.t1 +pf_a_cp048715 AUGUSTUS start_codon 9802 9804 . - 0 Parent=g7.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccgggacagcacgctgatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtcgtttcggcacatcctgaacatcaccaccaggagacttcatccaaggacgagcag +# ctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccatttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaatcttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatcagtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtt +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gatactgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgaggtccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaacgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgtgcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgagggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g7 +### +# start gene g8 +pf_a_cp048715 AUGUSTUS gene 9860 11175 0.94 + . ID=g8 +pf_a_cp048715 AUGUSTUS transcript 9860 11175 0.94 + . ID=g8.t1;Parent=g8 +pf_a_cp048715 AUGUSTUS start_codon 9860 9862 . + 0 Parent=g8.t1 +pf_a_cp048715 AUGUSTUS intron 10029 10456 0.94 + . Parent=g8.t1 +pf_a_cp048715 AUGUSTUS CDS 9860 10028 0.95 + 0 ID=g8.t1.cds;Parent=g8.t1 +pf_a_cp048715 AUGUSTUS CDS 10457 11172 0.96 + 2 ID=g8.t1.cds;Parent=g8.t1 +pf_a_cp048715 AUGUSTUS stop_codon 11173 11175 . + 0 Parent=g8.t1 +# coding sequence = [atggcggaggaaagttttagtggctcggatctcacggattaccgctccggccgaggcgacccgcagcaatggagatcaa +# cagatgcagtcattagacacacaggcttccagtcgaggctacgcgagcattggtggtcctcacgagaaacagaaagggataggctgtgctatctcaca +# cctctcgaaaatgcatacttcaactaccaggaggaacttgaacgcagactgatgtggacattcccacactactactacttcaagaagggttccctcgc +# cgaacgtcgtttcttcaaggcacagcgtggacctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaac +# gtcgctcgaagcaggaggttgtgattccaaaggatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttaca +# aaagcagacgaacagggtgatttgacaagtttggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgcc +# aagtttcgacgttacagagggtacaccattgcacagagtcgccgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctc +# gttctccaacggcagtcttaaagtttgatggctgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaat +# atcctctcaggtgcattcaagccacagaaggttgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaac +# tgagtttttgttctctcgtgtctga] +# protein sequence = [MAEESFSGSDLTDYRSGRGDPQQWRSTDAVIRHTGFQSRLREHWWSSRETERDRLCYLTPLENAYFNYQEELERRLMW +# TFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNSRVTKADEQGDLTSLERQLPRTL +# YLLVKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFIIKSNILSGAFKPQKVEYAWLTRE +# EIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 2 +# RM: 2 +# incompatible hint groups: 2 +# RM: 2 +# end gene g8 +### +# start gene g9 +pf_a_cp048715 AUGUSTUS gene 11319 12716 1 - . ID=g9 +pf_a_cp048715 AUGUSTUS transcript 11319 12716 1 - . ID=g9.t1;Parent=g9 +pf_a_cp048715 AUGUSTUS stop_codon 11319 11321 . - 0 Parent=g9.t1 +pf_a_cp048715 AUGUSTUS CDS 11322 12716 1 - 0 ID=g9.t1.cds;Parent=g9.t1 +pf_a_cp048715 AUGUSTUS start_codon 12714 12716 . - 0 Parent=g9.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggtgacgctcgaggaagtggtagaggagaaggaagaggtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaatgtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatttttgtcggtcttgtga +# ccggtaagatctctgttgttatcaagggcggtgatggtgcctttgccaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggcatcatctcactctggtccaccacagacctatcattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccatcttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRGNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHECMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFANACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLSLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g9 +### +# start gene g10 +pf_a_cp048715 AUGUSTUS gene 12921 14789 0.52 + . ID=g10 +pf_a_cp048715 AUGUSTUS transcript 12921 14789 0.52 + . ID=g10.t1;Parent=g10 +pf_a_cp048715 AUGUSTUS start_codon 12921 12923 . + 0 Parent=g10.t1 +pf_a_cp048715 AUGUSTUS CDS 12921 14786 0.52 + 0 ID=g10.t1.cds;Parent=g10.t1 +pf_a_cp048715 AUGUSTUS stop_codon 14787 14789 . + 0 Parent=g10.t1 +# coding sequence = [atgaattcccaacggcatacaccctatattcgagctgactcctccagtgtgctaccgaggacgctgggccgtcgaccgc +# ccggccctgtcatcccgaacctgggagctaagcaccatcacagctctcttaacaaaccccctcgatcatctcgacagcatgatgaacgagagtcttca +# tcacctcccagaccacacagctcctttgccccacctatcaactccaattcctcaaccacgtccgcctctagtcccaaaaaaaacgttaccgatgggcg +# ggaccctgccaacttgatctccacaacgtacgcctactgcggcaacaggaaacgactgcagaatccaagggatgtcgaaaataaaaagagactaagag +# agatggaagcaaaaaatgctcctggggaaccgccggtggctctacagtctaaaaggtttcaacaggaagcgtcagttgtcaaggcgaatgatggggac +# acctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctttggagaaacgtgccccaat +# aatgtctccaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcgatcagacgctgacttcatgaactccattcgtgctccaagga +# tccccagtcgagctgctcaagctgactacgagaagcacactgctagcgctcgtctacaacataaacgtgaggatacagaggtcaagggaatggatcaa +# caattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcgctgattaaaggtgtgaa +# atcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtcagggagtaagtcacctc +# accgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccggggccgtcatgaaggttcagtgggggacgctctaggc +# tcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaaggatgatgaaagtcataaagc +# tatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatcttgagatgatgaagcgca +# ggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatgagtttgaagaggttgac +# gagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagtttccgggaacattaaccctcggtgagattgagcaaaa +# ggtcaaccagcacatggactgcattccaaaaattctcaacggtcgtcttgactccacttacctgcaaaaagcgaaagtattcatgcacgactctggag +# caacaatcttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaaaagacaaggggctattgctaatcta +# atcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattcacattacacattctcgc +# tccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagttgattatggtcaataca +# tcgctgatttggtcccttgtgaatga] +# protein sequence = [MNSQRHTPYIRADSSSVLPRTLGRRPPGPVIPNLGAKHHHSSLNKPPRSSRQHDERESSSPPRPHSSFAPPINSNSST +# TSASSPKKNVTDGRDPANLISTTYAYCGNRKRLQNPRDVENKKRLREMEAKNAPGEPPVALQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNR +# KRNILSSLEKRAPIMSPGEEEQEGSRHRIPSIRSDADFMNSIRAPRIPSRAAQADYEKHTASARLQHKREDTEVKGMDQQLHDDTNSGKRETKKGEDV +# APIPRKRALIKGVKSSAKKSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRGRHEGSVGDALGSDEELFNFDSPGPSSQELR +# SAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNE +# EFPGTLTLGEIEQKVNQHMDCIPKILNGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVI +# QFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g10 +### +# start gene g11 +pf_a_cp048715 AUGUSTUS gene 15247 18372 1 + . ID=g11 +pf_a_cp048715 AUGUSTUS transcript 15247 18372 1 + . ID=g11.t1;Parent=g11 +pf_a_cp048715 AUGUSTUS start_codon 15247 15249 . + 0 Parent=g11.t1 +pf_a_cp048715 AUGUSTUS CDS 15247 18369 1 + 0 ID=g11.t1.cds;Parent=g11.t1 +pf_a_cp048715 AUGUSTUS stop_codon 18370 18372 . + 0 Parent=g11.t1 +# coding sequence = [atgtcgaagacaatctccgactccgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctatggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgccgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctctaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagttagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaaaaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaaaaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacctgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacatcaatggtgatcttggtgatgcagttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctattgccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgaccaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcttccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagtgaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacgtctgcctgcagatctttggtcaacca +# agttcaggatattttgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagatcagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAIAVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 18 +# RM: 18 +# end gene g11 +### +# +# ----- prediction on sequence number 2 (length = 19501, name = pf_a_jcm3601) ----- +# +# Predicted genes for sequence number 2 on both strands +# start gene g12 +pf_a_jcm3601 AUGUSTUS gene 944 1801 0.89 - . ID=g12 +pf_a_jcm3601 AUGUSTUS transcript 944 1801 0.89 - . ID=g12.t1;Parent=g12 +pf_a_jcm3601 AUGUSTUS stop_codon 944 946 . - 0 Parent=g12.t1 +pf_a_jcm3601 AUGUSTUS CDS 947 1801 0.89 - 0 ID=g12.t1.cds;Parent=g12.t1 +pf_a_jcm3601 AUGUSTUS start_codon 1799 1801 . - 0 Parent=g12.t1 +# coding sequence = [atgattagtactaacagtcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctgaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgacgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgaatctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNSHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYESINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g12 +### +# start gene g13 +pf_a_jcm3601 AUGUSTUS gene 2148 3074 0.98 - . ID=g13 +pf_a_jcm3601 AUGUSTUS transcript 2148 3074 0.98 - . ID=g13.t1;Parent=g13 +pf_a_jcm3601 AUGUSTUS stop_codon 2148 2150 . - 0 Parent=g13.t1 +pf_a_jcm3601 AUGUSTUS CDS 2151 3074 0.98 - 0 ID=g13.t1.cds;Parent=g13.t1 +pf_a_jcm3601 AUGUSTUS start_codon 3072 3074 . - 0 Parent=g13.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtgg +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttttctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggttgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctggt +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVAPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g13 +### +# start gene g14 +pf_a_jcm3601 AUGUSTUS gene 3549 3941 0.52 - . ID=g14 +pf_a_jcm3601 AUGUSTUS transcript 3549 3941 0.52 - . ID=g14.t1;Parent=g14 +pf_a_jcm3601 AUGUSTUS stop_codon 3549 3551 . - 0 Parent=g14.t1 +pf_a_jcm3601 AUGUSTUS CDS 3552 3941 0.52 - 0 ID=g14.t1.cds;Parent=g14.t1 +pf_a_jcm3601 AUGUSTUS start_codon 3939 3941 . - 0 Parent=g14.t1 +# coding sequence = [atgtcagttatctttttgaacgatccggaaaactcaatcaagcagagtaagcaaaagaccgaaaaagcttcagctagtg +# atatcaaagatatgagcagttatgtcaagctttgcactagtgtttatgaggaagacgactgcaaacggaggggggtcgatgctctccacgattcagat +# gactacaaagaccatgtgcttgtattaacgcttaacgagcctaatatgaaagctccggttggctctgctagtacaaacgccaggacaacatataaggt +# aacaaagcccacaaaaaagaagaagacgccgcctcatttggagcttgtattcagtaaattcaggtcccctttgtatggtgaagccgaggatatttgca +# tcaacagttcccctatgtga] +# protein sequence = [MSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAP +# VGSASTNARTTYKVTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g14 +### +# start gene g15 +pf_a_jcm3601 AUGUSTUS gene 5136 5835 0.37 + . ID=g15 +pf_a_jcm3601 AUGUSTUS transcript 5136 5835 0.37 + . ID=g15.t1;Parent=g15 +pf_a_jcm3601 AUGUSTUS start_codon 5136 5138 . + 0 Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS intron 5437 5563 0.37 + . Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS CDS 5136 5436 0.37 + 0 ID=g15.t1.cds;Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS CDS 5564 5832 0.38 + 2 ID=g15.t1.cds;Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 5833 5835 . + 0 Parent=g15.t1 +# coding sequence = [atggccacgagggatcaaatagcgtataaggaggcaagcgagaagcttgatgcccttatcgagttttcatctaacttct +# cgactaacccaacgaaaccgagatatgacaaaataatgctggaagatccgtttgagaaggagagtttactgagcaaggttccggcctatgcgagagca +# gcattggaaactctgatctcaaacttcgaactatccctcggacgtgttatggtaggacaatctctttcccataaatctaaccaatcggtcataaaaac +# taacaaaccattaggaccacgactcacatcgtacgaagtccagtctaccactatcgaagtaacgggatcgacttctagccctgtctttccaggtggag +# gtcgcaagcgaaatgcgcttccagcaaagaagcgaacatcaattacccctgaatccaaggagtttttggagagagtgtttaaggctaagaggactcca +# aacagtacggagaggaaagttatagcagaaaagtgtggactcacgccactgcaggttagggtatggttcacaaacaagagaatgaggaacaaggaagg +# ctga] +# protein sequence = [MATRDQIAYKEASEKLDALIEFSSNFSTNPTKPRYDKIMLEDPFEKESLLSKVPAYARAALETLISNFELSLGRVMVG +# QSLSHKSNQSVIKTNKPLGPRLTSYEVQSTTIEVTGSTSSPVFPGGGRKRNALPAKKRTSITPESKEFLERVFKAKRTPNSTERKVIAEKCGLTPLQV +# RVWFTNKRMRNKEG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 1 +# RM: 1 +# incompatible hint groups: 1 +# RM: 1 +# end gene g15 +### +# start gene g16 +pf_a_jcm3601 AUGUSTUS gene 5937 7166 1 - . ID=g16 +pf_a_jcm3601 AUGUSTUS transcript 5937 7166 1 - . ID=g16.t1;Parent=g16 +pf_a_jcm3601 AUGUSTUS stop_codon 5937 5939 . - 0 Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS CDS 5940 7166 1 - 0 ID=g16.t1.cds;Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS start_codon 7164 7166 . - 0 Parent=g16.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctactatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggctcttggtgctattgtcaaaagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaactag] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g16 +### +# start gene g17 +pf_a_jcm3601 AUGUSTUS gene 7472 7993 1 + . ID=g17 +pf_a_jcm3601 AUGUSTUS transcript 7472 7993 1 + . ID=g17.t1;Parent=g17 +pf_a_jcm3601 AUGUSTUS start_codon 7472 7474 . + 0 Parent=g17.t1 +pf_a_jcm3601 AUGUSTUS CDS 7472 7990 1 + 0 ID=g17.t1.cds;Parent=g17.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 7991 7993 . + 0 Parent=g17.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgaaatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g17 +### +# start gene g18 +pf_a_jcm3601 AUGUSTUS gene 8231 10000 0.92 - . ID=g18 +pf_a_jcm3601 AUGUSTUS transcript 8231 10000 0.92 - . ID=g18.t1;Parent=g18 +pf_a_jcm3601 AUGUSTUS stop_codon 8231 8233 . - 0 Parent=g18.t1 +pf_a_jcm3601 AUGUSTUS CDS 8234 10000 0.92 - 0 ID=g18.t1.cds;Parent=g18.t1 +pf_a_jcm3601 AUGUSTUS start_codon 9998 10000 . - 0 Parent=g18.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggacagcacgctgatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcacgaccaggagacttcatccaaggacgagcag +# ctggagaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccatttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaggcatttacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtc +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcaaacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaatgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgtgcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgagggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHDQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g18 +### +# start gene g19 +pf_a_jcm3601 AUGUSTUS gene 10711 11370 0.66 + . ID=g19 +pf_a_jcm3601 AUGUSTUS transcript 10711 11370 0.66 + . ID=g19.t1;Parent=g19 +pf_a_jcm3601 AUGUSTUS start_codon 10711 10713 . + 0 Parent=g19.t1 +pf_a_jcm3601 AUGUSTUS CDS 10711 11367 0.66 + 0 ID=g19.t1.cds;Parent=g19.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 11368 11370 . + 0 Parent=g19.t1 +# coding sequence = [atgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggac +# ctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaag +# gatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagttt +# ggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcgacgttacagagggtacaccattgc +# acagagtcgccgaggagggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttgaagtttgatggc +# tgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggt +# tgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNS +# RVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEEGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFII +# KSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g19 +### +# start gene g20 +pf_a_jcm3601 AUGUSTUS gene 11514 12911 1 - . ID=g20 +pf_a_jcm3601 AUGUSTUS transcript 11514 12911 1 - . ID=g20.t1;Parent=g20 +pf_a_jcm3601 AUGUSTUS stop_codon 11514 11516 . - 0 Parent=g20.t1 +pf_a_jcm3601 AUGUSTUS CDS 11517 12911 1 - 0 ID=g20.t1.cds;Parent=g20.t1 +pf_a_jcm3601 AUGUSTUS start_codon 12909 12911 . - 0 Parent=g20.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggcgacgctcgaggaagtggtagaggggaaggaagaagtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatatttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccaccttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRSNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g20 +### +# start gene g21 +pf_a_jcm3601 AUGUSTUS gene 13491 14984 0.86 + . ID=g21 +pf_a_jcm3601 AUGUSTUS transcript 13491 14984 0.86 + . ID=g21.t1;Parent=g21 +pf_a_jcm3601 AUGUSTUS start_codon 13491 13493 . + 0 Parent=g21.t1 +pf_a_jcm3601 AUGUSTUS CDS 13491 14981 0.86 + 0 ID=g21.t1.cds;Parent=g21.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 14982 14984 . + 0 Parent=g21.t1 +# coding sequence = [atggaagcaaaaaatgctcctggggaaccgccggtgcctctacagtctaaaaggtttcaacaggaagcgtcagttgtca +# aggcgaatgatggggacacctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctagg +# gagaaacgtgccccaataatgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaactc +# cattcgtgctccaaggattcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagagg +# tcaaggaaatggatcaacaattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcg +# ctgattaaaggtgtgaaatcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtc +# agggagtaagtcacctcaccgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttcag +# tgggggacgctctaggctcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaaggat +# gatgaaagtcataaagctatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatct +# tgagatgatgaaacgcaggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatg +# agtttgaagaggttgacgagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacattaagcctc +# ggtgagattgagcaaaaggccaaccagcacatggactgcattccaaaaattctcgacggtcgtcttgactccacttacctacaaaaagcgaaagtatt +# catgcacgactctggagcaacaattttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaag +# gggctattgccaatctgatcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattc +# acattacacattctcgctccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagt +# tgattatggtcaatacatcgctgatttggtcccttgtgaatga] +# protein sequence = [MEAKNAPGEPPVPLQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNRKRNILSSREKRAPIMSSGEEEQEGSRH +# RIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRETKKGEDVAPIPRKRALIKGVKSSAKKSTGSTSEP +# LGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGPSSQELRSAKELLKDDESHKAMKSAIDSLSIKSG +# ILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNEEFPGTLSLGEIEQKANQHMDCIPKILD +# GRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVIQFWSIDTFTLHILAPEVAARMAAEDMS +# ISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 6 +# RM: 6 +# end gene g21 +### +# start gene g22 +pf_a_jcm3601 AUGUSTUS gene 15442 18567 1 + . ID=g22 +pf_a_jcm3601 AUGUSTUS transcript 15442 18567 1 + . ID=g22.t1;Parent=g22 +pf_a_jcm3601 AUGUSTUS start_codon 15442 15444 . + 0 Parent=g22.t1 +pf_a_jcm3601 AUGUSTUS CDS 15442 18564 1 + 0 ID=g22.t1.cds;Parent=g22.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 18565 18567 . + 0 Parent=g22.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatatggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagcagctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatgttgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDVDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g22 +### +# +# ----- prediction on sequence number 3 (length = 22001, name = pf_alpha_strain65) ----- +# +# Predicted genes for sequence number 3 on both strands +# start gene g23 +pf_alpha_strain65 AUGUSTUS gene 704 1561 0.9 - . ID=g23 +pf_alpha_strain65 AUGUSTUS transcript 704 1561 0.9 - . ID=g23.t1;Parent=g23 +pf_alpha_strain65 AUGUSTUS stop_codon 704 706 . - 0 Parent=g23.t1 +pf_alpha_strain65 AUGUSTUS CDS 707 1561 0.9 - 0 ID=g23.t1.cds;Parent=g23.t1 +pf_alpha_strain65 AUGUSTUS start_codon 1559 1561 . - 0 Parent=g23.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgatgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgactctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g23 +### +# start gene g24 +pf_alpha_strain65 AUGUSTUS gene 1908 2834 0.96 - . ID=g24 +pf_alpha_strain65 AUGUSTUS transcript 1908 2834 0.96 - . ID=g24.t1;Parent=g24 +pf_alpha_strain65 AUGUSTUS stop_codon 1908 1910 . - 0 Parent=g24.t1 +pf_alpha_strain65 AUGUSTUS CDS 1911 2834 0.96 - 0 ID=g24.t1.cds;Parent=g24.t1 +pf_alpha_strain65 AUGUSTUS start_codon 2832 2834 . - 0 Parent=g24.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttttctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g24 +### +# start gene g25 +pf_alpha_strain65 AUGUSTUS gene 3495 5891 0.98 + . ID=g25 +pf_alpha_strain65 AUGUSTUS transcript 3495 5891 0.98 + . ID=g25.t1;Parent=g25 +pf_alpha_strain65 AUGUSTUS start_codon 3495 3497 . + 0 Parent=g25.t1 +pf_alpha_strain65 AUGUSTUS CDS 3495 5888 0.98 + 0 ID=g25.t1.cds;Parent=g25.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 5889 5891 . + 0 Parent=g25.t1 +# coding sequence = [atgttgtcccaccgccatcagaaagctgctctctccaaactgcaaagaagtgcttacgcacattacccaacaccacatt +# cgccaatatctctaacaccaaatagatcaatatcgtctcttccggcctcgaagatgcttccagctgtcaattacgttcgccaagcagatccagatgcg +# attgttcttctcggtcatggtgaatcttaccatgctttcggcagagtcgatactaatctgattaaaggaatggtatcagctggcaatgcaagcgagat +# actctaccgtgatgatggactgacacaatggagattcaagtcatctcgactcccgcataacacttcttggctggtgaaggagttgaacaggccagttg +# tcaccttggaatggaacgaatatgctactagtttcctacagccattgcgcaacgactcccattggcatttcctatcgagaaccattcctgatggccac +# tcagaccatgaattctctgttcattctgaatctcaaaacatcatagtaattggtatacaggggaagattctcaaggggatcaaaacaggtgaaaagtc +# acagagacgtgttggtatcacttgctttgacccaacaaacggatcgatgtctgttcagggcacttctcttgctgatttgagatcccatatcgtgagct +# ttcgtcctcgggctattgttctggactccagtctgattgggacataccgggacatcattggcacagctcttctaggttcaccagaagttaagtttacc +# aatatgcctcccgtgttgggctcgctacaactccttaatgatttctttgatgtctctggtgaaatttggaggaagtgtttcgatgattacgagctgta +# ctcactgaaggctgctgtggagttcattaagaagacacagaaatcaacgtctgcagtattccaactaccatcgagggaactgtcgtcacaatggaccg +# tgttattggacaaagaaactatcagttccttgaacctgggctccaccgggagtcactatatgaattcctcctggttacttcaaagatcaatcaagact +# gctactgttggtggttctcgaatgttgtacaagtctatcttgaggccaactaggatagtcagtgagattttgtttcgtcagagggcggttcaatccat +# cattaaaaggaaacatacacgtcagaagctgagctcagagctctccagtttaccagacatcccattgctactacagaaactcaagatctatccggaat +# cagaagaccttgtcagtactatactacactccatcgacatcatctcgtcacttgcccccatcgcctccaagttttacatgaacggtcaccagcaagac +# gctgactttgatgatggtgacttgatttaccgcattccatctgcaaaacgtttggaggaactcagagacagggcccgttcccttctcaatgctagcga +# cacaatgcatggtgttgtttcctcgaatacaagcattgaatgcaatgatcggaggacacagaaagctactttggtgaatggtgttgattgtggaccta +# ataggaacacttctttttctcactctgtgaaaaatcagacaggtgatgtttctttggaaacctttatggtcagcgatgatgctgaattgaacactgag +# tcaccttctaatctcacaacagtgctagacacccgtactgtgagtgaacacacccagcctaaggggctaggagagattaccagctcatctttggacta +# ccgagatctacatgctacacaccgcatcgacaagttggccggtcagataagtgaattacaccctggccaccctctaccaagagccaaagtgattgcca +# actgctcggctgaaatattaagacataaggagctcattctactcttctcccgactcttgtcacgtttggacatgctgctgtcgtttgccaacgtggct +# atccaacatgacgacacaagacctatcgtggatcccagcagaaccaactttgagattcacttgaaggacaaagatttcaatgctgctgctgatccatt +# gttgatgtcaaactcatcatcagtattgtggactaagggagagagaaaaaccgtggcagagctttacgggctgtacgccagtatcatcttattggcgc +# actcaggtgcttatgttccagccaagtctgctaacattggtgttgtcagccacatgctttgtcaactccgctctacaaatcctttcctattacattca +# gcgcttgagaagtctatggatggccgtcgtatcatcatgtgcgacgctctgcctgacgatttcgaagactatggctcagccgatgacagattggaaag +# ctaccaagaaacactggcgactatgaatgtgggaactaaccgactgcttctaagatatcactga] +# protein sequence = [MLSHRHQKAALSKLQRSAYAHYPTPHSPISLTPNRSISSLPASKMLPAVNYVRQADPDAIVLLGHGESYHAFGRVDTN +# LIKGMVSAGNASEILYRDDGLTQWRFKSSRLPHNTSWLVKELNRPVVTLEWNEYATSFLQPLRNDSHWHFLSRTIPDGHSDHEFSVHSESQNIIVIGI +# QGKILKGIKTGEKSQRRVGITCFDPTNGSMSVQGTSLADLRSHIVSFRPRAIVLDSSLIGTYRDIIGTALLGSPEVKFTNMPPVLGSLQLLNDFFDVS +# GEIWRKCFDDYELYSLKAAVEFIKKTQKSTSAVFQLPSRELSSQWTVLLDKETISSLNLGSTGSHYMNSSWLLQRSIKTATVGGSRMLYKSILRPTRI +# VSEILFRQRAVQSIIKRKHTRQKLSSELSSLPDIPLLLQKLKIYPESEDLVSTILHSIDIISSLAPIASKFYMNGHQQDADFDDGDLIYRIPSAKRLE +# ELRDRARSLLNASDTMHGVVSSNTSIECNDRRTQKATLVNGVDCGPNRNTSFSHSVKNQTGDVSLETFMVSDDAELNTESPSNLTTVLDTRTVSEHTQ +# PKGLGEITSSSLDYRDLHATHRIDKLAGQISELHPGHPLPRAKVIANCSAEILRHKELILLFSRLLSRLDMLLSFANVAIQHDDTRPIVDPSRTNFEI +# HLKDKDFNAAADPLLMSNSSSVLWTKGERKTVAELYGLYASIILLAHSGAYVPAKSANIGVVSHMLCQLRSTNPFLLHSALEKSMDGRRIIMCDALPD +# DFEDYGSADDRLESYQETLATMNVGTNRLLLRYH] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g25 +### +# start gene g26 +pf_alpha_strain65 AUGUSTUS gene 7801 8718 0.88 + . ID=g26 +pf_alpha_strain65 AUGUSTUS transcript 7801 8718 0.88 + . ID=g26.t1;Parent=g26 +pf_alpha_strain65 AUGUSTUS start_codon 7801 7803 . + 0 Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS CDS 7801 8715 0.88 + 0 ID=g26.t1.cds;Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 8716 8718 . + 0 Parent=g26.t1 +# coding sequence = [atgattgaagttcctctcggagcacttttggctcttagtgatccatacaccttctcttcacaaggtaaaatgaccggta +# tatcaaagatagtgaagaagcgaaacaaacaaggagttgaggttcttcgtttccgcggatgcccagcgtctaaagtgaggggcacaaggggtgcccaa +# ggactgcatcagtgtatggaaaggaacaattccatgaccagtggagaagcatccgtaacaatttttgctgctacgaagcttcctgatataccaagcat +# accgtatactttacagaagcagctggatgagctccgctctcagacaaccacatcagaccgcaggaagatcaaacgccttccccagctgcaagtgtcgg +# tgaacagagatgatacggttattaaaagggccaccaaaaccaaactaaatgggtttatggctttccgggcatttcattctcgtgaaatttctactatt +# gaacaccaaaggggtatttcgatgtcactggcgactgtgtgggcgagcgatcctccctcgcaacttgtctggcaaagattcgcagcagaatataacca +# aacagtacggtatgagcccgatttgtcatttgttgactggcttctttcaatgtcgaagggggttgactcgaaaattgatgaaccaacaaagagaacct +# taagtagggcagccagggtggagaacatttatgttgagaaggacggaaatctaagacacaacacagatccatatgctcttgaactccaaaataaggac +# gttaacaaccgttatcataactacttgaaggaaagattgcagtcggcaatagatcgtccaattgtcacactcgctagtgacccatcgttatccaggtc +# atatatagatccactagtctatgagatcagtagtggcacttacgaaaaatcctaa] +# protein sequence = [MIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASV +# TIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPP +# SQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDR +# PIVTLASDPSLSRSYIDPLVYEISSGTYEKS] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g26 +### +# start gene g27 +pf_alpha_strain65 AUGUSTUS gene 8788 10017 1 - . ID=g27 +pf_alpha_strain65 AUGUSTUS transcript 8788 10017 1 - . ID=g27.t1;Parent=g27 +pf_alpha_strain65 AUGUSTUS stop_codon 8788 8790 . - 0 Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS CDS 8791 10017 1 - 0 ID=g27.t1.cds;Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS start_codon 10015 10017 . - 0 Parent=g27.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacatacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctattatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggcgcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaattga] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g27 +### +# start gene g28 +pf_alpha_strain65 AUGUSTUS gene 10323 10844 1 + . ID=g28 +pf_alpha_strain65 AUGUSTUS transcript 10323 10844 1 + . ID=g28.t1;Parent=g28 +pf_alpha_strain65 AUGUSTUS start_codon 10323 10325 . + 0 Parent=g28.t1 +pf_alpha_strain65 AUGUSTUS CDS 10323 10841 1 + 0 ID=g28.t1.cds;Parent=g28.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 10842 10844 . + 0 Parent=g28.t1 +# coding sequence = [atgaacagcataacccaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g28 +### +# start gene g29 +pf_alpha_strain65 AUGUSTUS gene 11081 12850 0.94 - . ID=g29 +pf_alpha_strain65 AUGUSTUS transcript 11081 12850 0.94 - . ID=g29.t1;Parent=g29 +pf_alpha_strain65 AUGUSTUS stop_codon 11081 11083 . - 0 Parent=g29.t1 +pf_alpha_strain65 AUGUSTUS CDS 11084 12850 0.94 - 0 ID=g29.t1.cds;Parent=g29.t1 +pf_alpha_strain65 AUGUSTUS start_codon 12848 12850 . - 0 Parent=g29.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggacagcacgctgatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcaccaccaggagacttcatccaaggacgagcag +# ctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccgtttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtc +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaatgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgttcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgaaggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g29 +### +# start gene g30 +pf_alpha_strain65 AUGUSTUS gene 13310 14224 0.38 + . ID=g30 +pf_alpha_strain65 AUGUSTUS transcript 13310 14224 0.38 + . ID=g30.t1;Parent=g30 +pf_alpha_strain65 AUGUSTUS start_codon 13310 13312 . + 0 Parent=g30.t1 +pf_alpha_strain65 AUGUSTUS intron 13332 13505 0.57 + . Parent=g30.t1 +pf_alpha_strain65 AUGUSTUS CDS 13310 13331 0.66 + 0 ID=g30.t1.cds;Parent=g30.t1 +pf_alpha_strain65 AUGUSTUS CDS 13506 14221 0.58 + 2 ID=g30.t1.cds;Parent=g30.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 14222 14224 . + 0 Parent=g30.t1 +# coding sequence = [atgatgcagaatggtcttcagcatctcacacctctcgaaaatgcatacttcaactaccaggaggaactcgaacgcagac +# tgatgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggacctgtatctaagcagcca +# ggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaaggatgaatacgaagaatc +# cgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagtttggaaagacagctaccga +# gaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcgacgttacagagggtacaccattgcacagagtcgctgaggac +# ggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttaaagtttgatggctgctctttggctccagt +# ggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggttgagtatgcttggttga +# ctcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MMQNGLQHLTPLENAYFNYQEELERRLMWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRS +# KQEVVIPKDEYEESAMSDISRPVLPNSRVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSP +# TAVLKFDGCSLAPVDAQKKGLSREFIIKSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 1 +# RM: 1 +# incompatible hint groups: 2 +# RM: 2 +# end gene g30 +### +# start gene g31 +pf_alpha_strain65 AUGUSTUS gene 14368 15765 1 - . ID=g31 +pf_alpha_strain65 AUGUSTUS transcript 14368 15765 1 - . ID=g31.t1;Parent=g31 +pf_alpha_strain65 AUGUSTUS stop_codon 14368 14370 . - 0 Parent=g31.t1 +pf_alpha_strain65 AUGUSTUS CDS 14371 15765 1 - 0 ID=g31.t1.cds;Parent=g31.t1 +pf_alpha_strain65 AUGUSTUS start_codon 15763 15765 . - 0 Parent=g31.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggcgacgctcgaggaagtggtagaggggaaggaagaagtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccttcaactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaggagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatttttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccatcttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRSNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDRSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g31 +### +# start gene g32 +pf_alpha_strain65 AUGUSTUS gene 16345 17838 0.97 + . ID=g32 +pf_alpha_strain65 AUGUSTUS transcript 16345 17838 0.97 + . ID=g32.t1;Parent=g32 +pf_alpha_strain65 AUGUSTUS start_codon 16345 16347 . + 0 Parent=g32.t1 +pf_alpha_strain65 AUGUSTUS CDS 16345 17835 0.97 + 0 ID=g32.t1.cds;Parent=g32.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 17836 17838 . + 0 Parent=g32.t1 +# coding sequence = [atggaagcaaaaaatgctcctggggaaccgccggtgcctctacagtctaaaaggtttcaacaggaagcgtcagttgtca +# aggcgaatgatggggacacctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctagg +# gagaaacgtgccccaataatgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaactc +# cattcgtgctccaaggattcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagagg +# tcaaggaaatggatcaacaattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcg +# ctgattaaaggtgtgaaatcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtc +# agggagtaagtcacctcaccgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttcag +# tgggggacgctctaggctcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaaggat +# gatgaaagtcataaagctatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatct +# tgagatgatgaagcgcaggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatg +# agtttgaagaggttgacgagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacactaagcctc +# ggtgagattgagcaaaaggccaaccagcacatggactgcattccaaaaattctcgacggtcgtcttgactccacttacctacaaaaagcgaaagtatt +# catgcacgactctggagcaacaattttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaag +# gggctattgccaatctgatcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattc +# acattacacattctcgctccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagt +# tgattatggtcaatacatcgctgatttggtcccttgtgaatga] +# protein sequence = [MEAKNAPGEPPVPLQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNRKRNILSSREKRAPIMSSGEEEQEGSRH +# RIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRETKKGEDVAPIPRKRALIKGVKSSAKKSTGSTSEP +# LGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGPSSQELRSAKELLKDDESHKAMKSAIDSLSIKSG +# ILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNEEFPGTLSLGEIEQKANQHMDCIPKILD +# GRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVIQFWSIDTFTLHILAPEVAARMAAEDMS +# ISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 6 +# RM: 6 +# end gene g32 +### +# start gene g33 +pf_alpha_strain65 AUGUSTUS gene 18296 21421 1 + . ID=g33 +pf_alpha_strain65 AUGUSTUS transcript 18296 21421 1 + . ID=g33.t1;Parent=g33 +pf_alpha_strain65 AUGUSTUS start_codon 18296 18298 . + 0 Parent=g33.t1 +pf_alpha_strain65 AUGUSTUS CDS 18296 21418 1 + 0 ID=g33.t1.cds;Parent=g33.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 21419 21421 . + 0 Parent=g33.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcagaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaccgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaga +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacctcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYETDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g33 +### +# +# ----- prediction on sequence number 4 (length = 22501, name = pf_alpha_tbrc4498) ----- +# +# Predicted genes for sequence number 4 on both strands +# start gene g34 +pf_alpha_tbrc4498 AUGUSTUS gene 943 1800 0.83 - . ID=g34 +pf_alpha_tbrc4498 AUGUSTUS transcript 943 1800 0.83 - . ID=g34.t1;Parent=g34 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 943 945 . - 0 Parent=g34.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 946 1800 0.83 - 0 ID=g34.t1.cds;Parent=g34.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 1798 1800 . - 0 Parent=g34.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaaccgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccactgcaagcaagaagaagtcgagtgcaaacttgagtgatgacgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgactctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAATASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g34 +### +# start gene g35 +pf_alpha_tbrc4498 AUGUSTUS gene 2147 3073 0.9 - . ID=g35 +pf_alpha_tbrc4498 AUGUSTUS transcript 2147 3073 0.9 - . ID=g35.t1;Parent=g35 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 2147 2149 . - 0 Parent=g35.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 2150 3073 0.9 - 0 ID=g35.t1.cds;Parent=g35.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 3071 3073 . - 0 Parent=g35.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccttcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttgtctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggcctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTFSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g35 +### +# start gene g36 +pf_alpha_tbrc4498 AUGUSTUS gene 3734 6130 0.96 + . ID=g36 +pf_alpha_tbrc4498 AUGUSTUS transcript 3734 6130 0.96 + . ID=g36.t1;Parent=g36 +pf_alpha_tbrc4498 AUGUSTUS start_codon 3734 3736 . + 0 Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 3734 6127 0.96 + 0 ID=g36.t1.cds;Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 6128 6130 . + 0 Parent=g36.t1 +# coding sequence = [atgttgtcccaccgccatcagaaagctgctctctccaaactgcaaagaagtgcttacgcacattacccaacaccacatt +# cgccaatatctctaacaccaaatagatcaatatcgtctcttccggcctcgaagatgcttccagctgtcaattacgttcgccaagcagatccagatgcg +# attgttcttctcggtcatggtgaatcttaccatgctttcggcagagtcgatactaatctgattaaaggaatggtatcagctggcaatgcaagcgagat +# actctaccgtgatgatggactgacacaatggagattcaagtcatctcgactcccgcataacacttcttggctggtgaaggagttgaacaggccagttg +# tcaccttggaatggaacgaatatgctactagtttcctacagccattgcgcaacgactcccattggcatttcctatcgagaaccattcctgatggccac +# tcagaccatgaattctctgttcattctgaatctcaaaacatcatagtaattggtatacaggggaagattctcaaggggatcaaaacaggtgaaaagtc +# acagagacgtgttggtatcacttgctttgacccaacaaacggatcgatgtctgttcagggcacttctcttgctgatttgagatcccatatcgtgagct +# ttcgtcctcgggctattgttctggactccagtctgattgggacataccgggacatcattggcacagctcttctaggttcaccagaagttaagtttacc +# aatatgcctcccgtgttgggctcgctacaactccttaatgatttctttgatgtctctggtgaaatttggaggaagtgtttcgatgattacgagctgta +# ctcactgaaggctgctgtggagttcattaagaagacacagaaatcaacgtctgcagtattccaactaccatcgagggaactgtcgtcacaatggaccg +# tgttattggacaaagaaactatcagttccttgaacctgggctccaccgggagtcactatatgaattcctcctggttacttcaaagatcaatcaagact +# gctactgttggtggttctcgaatgttgtacaagtctatcttgaggccaactaggatagtcagtgagattttgtttcgtcagagggcggttcaatccat +# cattaaaaggaaacatacacgtcagaagctgagctcagagctctcgagtttaccagacatcccattgctactacagaaactcaagatctatccggaat +# cagaagaccttgtcagtactatactacactccatcgacatcatctcgtcacttgctcccatcgcctccaagttttacatgaacggtcgccagcaagac +# gctgactttgatgatggtgacttgatttaccgcattccatctgcaaaacgtttggaggaactcagagacagggcccgttcccttctcaatgctagcga +# cacaatgcatggtgttgtttcctcgaatacaagcattgaatgcaatgatcggaggacacagaaagctactttggtgaatggtgttgattgtggaccta +# ataggaacacttctttttctcactctgtgaaaaatcagacaggtgatgtttctttggaaacctttatggtcagcgatgatgctgaattgaacactgag +# tcaccttctaatctcacaacagtgctagacacccgtactgtgagtgaacacacccagcctaaggggctaggagagattaccagctcatctttggacta +# ccgagatctacatgctacacaccgcatcgacaagttggccggtcagataagtgaattacaccctggccaccctctaccaagagccaaagtgattgcca +# actgctcggctgaaatattaagacataaggagctcattctactcttctcccgactcttgtcacgtttggacatgctgctgtcgtttgccaacgtggct +# atccaacatgacgacacaagacctatcgtggatcccagcagaaccaactttgagattcacttgaaggacaaagatttcaatgctgctgctgatccatt +# gttgatgtcaaactcatcatcagtattgtggactaagggagagagaaaaaccgtggcagagctttacgggctgtacgccagtatcatcttattggcgc +# actcaggtgcttatgttccagccaagtctgctaacattggtgttgtcagccacatgctttgtcaactccgctctacaaatcctttcctattacattca +# gcgcttgagaagtctatggatggccgtcgtatcatcatgtgcgacgctctgcctgacgatttcgaagactatggctcagccgatgacagattggaaag +# ctaccaagaaacactggcgactatgaatgtgggaactaaccgactgcttctaagatatcactga] +# protein sequence = [MLSHRHQKAALSKLQRSAYAHYPTPHSPISLTPNRSISSLPASKMLPAVNYVRQADPDAIVLLGHGESYHAFGRVDTN +# LIKGMVSAGNASEILYRDDGLTQWRFKSSRLPHNTSWLVKELNRPVVTLEWNEYATSFLQPLRNDSHWHFLSRTIPDGHSDHEFSVHSESQNIIVIGI +# QGKILKGIKTGEKSQRRVGITCFDPTNGSMSVQGTSLADLRSHIVSFRPRAIVLDSSLIGTYRDIIGTALLGSPEVKFTNMPPVLGSLQLLNDFFDVS +# GEIWRKCFDDYELYSLKAAVEFIKKTQKSTSAVFQLPSRELSSQWTVLLDKETISSLNLGSTGSHYMNSSWLLQRSIKTATVGGSRMLYKSILRPTRI +# VSEILFRQRAVQSIIKRKHTRQKLSSELSSLPDIPLLLQKLKIYPESEDLVSTILHSIDIISSLAPIASKFYMNGRQQDADFDDGDLIYRIPSAKRLE +# ELRDRARSLLNASDTMHGVVSSNTSIECNDRRTQKATLVNGVDCGPNRNTSFSHSVKNQTGDVSLETFMVSDDAELNTESPSNLTTVLDTRTVSEHTQ +# PKGLGEITSSSLDYRDLHATHRIDKLAGQISELHPGHPLPRAKVIANCSAEILRHKELILLFSRLLSRLDMLLSFANVAIQHDDTRPIVDPSRTNFEI +# HLKDKDFNAAADPLLMSNSSSVLWTKGERKTVAELYGLYASIILLAHSGAYVPAKSANIGVVSHMLCQLRSTNPFLLHSALEKSMDGRRIIMCDALPD +# DFEDYGSADDRLESYQETLATMNVGTNRLLLRYH] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g36 +### +# start gene g37 +pf_alpha_tbrc4498 AUGUSTUS gene 8041 8958 0.94 + . ID=g37 +pf_alpha_tbrc4498 AUGUSTUS transcript 8041 8958 0.94 + . ID=g37.t1;Parent=g37 +pf_alpha_tbrc4498 AUGUSTUS start_codon 8041 8043 . + 0 Parent=g37.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 8041 8955 0.94 + 0 ID=g37.t1.cds;Parent=g37.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 8956 8958 . + 0 Parent=g37.t1 +# coding sequence = [atgattgaagttcctctcggagcacttttggctcttagtgatccatacaccttctcttcacaaggtaaaatgaccggta +# tatcaaagattgtgaagaagcgaaacaaacaaggagttgaggttcttcgtttccgcggatgcccagcgtctaaagtgaggggcacaaggggtgcccaa +# ggactgcatcagtgtatggaaaggaacaattccatgaccagtggagaagcatccgtaacaatttttgctgctacgaagcttcctgatataccaagcat +# accgtatactttacagaagcagctggatgagctccgctctcagacaaccacatcagaccgcaggaagatcaaacgccttccccagctgcaagtgtcgg +# tgaacagagatgatacggttattaaaagggccaccaaaaccaaactaaatgggtttatggctttccgggcatttcattctcgtgaaatttctactatt +# gaacaccaaaggggtatttcgatgtcactggcgactgtgtgggcgagcgatcctccctcgcaacttgtctggcaaagattcgcagcagaatataacca +# aacagtacggtatgagcccgatttgtcatttgttgactggcttctttcaatgtcgaagggggttgactcgaaaattgatgaaccaacaaagagaacct +# taagtagggcagccagggtggagaacatttatgttgagaaggacggaaatctaagacacaacacagatccatatgctcttgaactccaaaataaggac +# gttaacaaccgttatcataactacttgaaggaaagattgcagtcggcaatagatcgtccaattgtcacactcgctagtgacccatcgttatccaggtc +# atatatagatccactagtctatgagatcagtagtggcacttacgaaaaatcctaa] +# protein sequence = [MIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASV +# TIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPP +# SQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDR +# PIVTLASDPSLSRSYIDPLVYEISSGTYEKS] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g37 +### +# start gene g38 +pf_alpha_tbrc4498 AUGUSTUS gene 9028 10257 1 - . ID=g38 +pf_alpha_tbrc4498 AUGUSTUS transcript 9028 10257 1 - . ID=g38.t1;Parent=g38 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 9028 9030 . - 0 Parent=g38.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 9031 10257 1 - 0 ID=g38.t1.cds;Parent=g38.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 10255 10257 . - 0 Parent=g38.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# ttggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctattatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggcgcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaattga] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g38 +### +# start gene g39 +pf_alpha_tbrc4498 AUGUSTUS gene 10563 11084 1 + . ID=g39 +pf_alpha_tbrc4498 AUGUSTUS transcript 10563 11084 1 + . ID=g39.t1;Parent=g39 +pf_alpha_tbrc4498 AUGUSTUS start_codon 10563 10565 . + 0 Parent=g39.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 10563 11081 1 + 0 ID=g39.t1.cds;Parent=g39.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 11082 11084 . + 0 Parent=g39.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g39 +### +# start gene g40 +pf_alpha_tbrc4498 AUGUSTUS gene 11321 13090 0.94 - . ID=g40 +pf_alpha_tbrc4498 AUGUSTUS transcript 11321 13090 0.94 - . ID=g40.t1;Parent=g40 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 11321 11323 . - 0 Parent=g40.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 11324 13090 0.94 - 0 ID=g40.t1.cds;Parent=g40.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 13088 13090 . - 0 Parent=g40.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggacagcacgcggatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcaccaccaggagacttcatccaaggacgagcag +# ctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccgtttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtc +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaacgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgttcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgaaggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g40 +### +# start gene g41 +pf_alpha_tbrc4498 AUGUSTUS gene 13550 14464 0.51 + . ID=g41 +pf_alpha_tbrc4498 AUGUSTUS transcript 13550 14464 0.51 + . ID=g41.t1;Parent=g41 +pf_alpha_tbrc4498 AUGUSTUS start_codon 13550 13552 . + 0 Parent=g41.t1 +pf_alpha_tbrc4498 AUGUSTUS intron 13572 13745 0.68 + . Parent=g41.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 13550 13571 0.66 + 0 ID=g41.t1.cds;Parent=g41.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 13746 14461 0.69 + 2 ID=g41.t1.cds;Parent=g41.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 14462 14464 . + 0 Parent=g41.t1 +# coding sequence = [atgatgcagaatggtcttcagcatctcacacctctcgaaaatgcatacttcaactaccaggaggaactcgaacgcagac +# tgatgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggacctgtatctaagcagcca +# ggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaaggatgaatacgaagaatc +# cgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagtttggaaagacagctaccga +# gaacactttatcttcttgtaaaggatggaaagcttggctggaaactgccaagtttcgacgttacagagggtacaccattgcacagagtcgctgaggac +# ggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttaaagtttgatggctgctctttggctccagt +# ggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggttgagtatgcttggttga +# ctcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MMQNGLQHLTPLENAYFNYQEELERRLMWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRS +# KQEVVIPKDEYEESAMSDISRPVLPNSRVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSP +# TAVLKFDGCSLAPVDAQKKGLSREFIIKSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 1 +# RM: 1 +# incompatible hint groups: 2 +# RM: 2 +# end gene g41 +### +# start gene g42 +pf_alpha_tbrc4498 AUGUSTUS gene 14608 16005 1 - . ID=g42 +pf_alpha_tbrc4498 AUGUSTUS transcript 14608 16005 1 - . ID=g42.t1;Parent=g42 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 14608 14610 . - 0 Parent=g42.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 14611 16005 1 - 0 ID=g42.t1.cds;Parent=g42.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 16003 16005 . - 0 Parent=g42.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggtgacgctcgaggaagtggtagaggggatggaagaggtaacagaggtgatagaggtgacagaaacagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtaagtgatcgtgctgatgctagtcgtcacacatcgagaagagacgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatatttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccaccttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGDGRGNRGDRGDRNRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADASRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g42 +### +# start gene g43 +pf_alpha_tbrc4498 AUGUSTUS gene 16585 18078 0.95 + . ID=g43 +pf_alpha_tbrc4498 AUGUSTUS transcript 16585 18078 0.95 + . ID=g43.t1;Parent=g43 +pf_alpha_tbrc4498 AUGUSTUS start_codon 16585 16587 . + 0 Parent=g43.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 16585 18075 0.95 + 0 ID=g43.t1.cds;Parent=g43.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 18076 18078 . + 0 Parent=g43.t1 +# coding sequence = [atggaagcaaaaaatgctcctggggaaccgccggtgcctctacagtctaaaaggtttcaacaggaagcgtcagttgtca +# aggcgaatgatggggacacctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctttg +# gagaaacgtgccccaataatgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaactc +# cattcgtgctccaaggattcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagagg +# tcaaggaaatggatcaacaattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcg +# ctgattaaaggtgtgaaatcttctgctaagaaaagcacaggatcgacgagtcaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtc +# agggagtaagtcacctcaccgccgtggcaccgaagacttgggtactgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttcag +# tgggggacgctctaggctcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaagaattgcgttccgccaaagagcttctcaaggat +# gatgaaagtcataaagctatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatct +# tgagatgatgaagcgcaggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatg +# agtttgaagaggttgacgagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacattaagcctc +# ggtgagattgagcaaaaggccaaccagcacatggactgcattccaaaaattctcaacggtcgtcttgactccacttacctacaaaaagcgaaagtatt +# catgcacgactctggagcaacaatcttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaag +# gggctattgccaatctgatcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattc +# acattacacattctcgctccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagt +# tgattatggtcaatacatcgctgatttggtcccttgtgaatga] +# protein sequence = [MEAKNAPGEPPVPLQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNRKRNILSSLEKRAPIMSSGEEEQEGSRH +# RIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRETKKGEDVAPIPRKRALIKGVKSSAKKSTGSTSQP +# LGDIRGMLSGKKKSGSKSPHRRGTEDLGTEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGPSSQELRSAKELLKDDESHKAMKSAIDSLSIKSG +# ILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNEEFPGTLSLGEIEQKANQHMDCIPKILN +# GRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVIQFWSIDTFTLHILAPEVAARMAAEDMS +# ISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g43 +### +# start gene g44 +pf_alpha_tbrc4498 AUGUSTUS gene 18536 21661 1 + . ID=g44 +pf_alpha_tbrc4498 AUGUSTUS transcript 18536 21661 1 + . ID=g44.t1;Parent=g44 +pf_alpha_tbrc4498 AUGUSTUS start_codon 18536 18538 . + 0 Parent=g44.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 18536 21658 1 + 0 ID=g44.t1.cds;Parent=g44.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 21659 21661 . + 0 Parent=g44.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgaaaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g44 +### +# command line: +# augustus --species=kluyveromyces_lactis --genemodel=complete --strand=both --protein=on --codingseq=on --gff3=on windows.fa diff --git a/results/2026-10-30_petasospora/data/aug_pichia_stipitis.gff3 b/results/2026-10-30_petasospora/data/aug_pichia_stipitis.gff3 new file mode 100644 index 00000000..5d4c527b --- /dev/null +++ b/results/2026-10-30_petasospora/data/aug_pichia_stipitis.gff3 @@ -0,0 +1,1508 @@ +##gff-version 3 +# This output was generated with AUGUSTUS (version 3.5.0). +# AUGUSTUS is a gene prediction tool written by M. Stanke (mario.stanke@uni-greifswald.de), +# O. Keller, S. König, L. Gerischer, L. Romoth, Katharina Hoff, Henry Mehlan and Daniel Honsel. +# Please cite: Mario Stanke, Mark Diekhans, Robert Baertsch, David Haussler (2008), +# Using native and syntenically mapped cDNA alignments to improve de novo gene finding +# Bioinformatics 24: 637-644, doi 10.1093/bioinformatics/btn013 +# No extrinsic information on sequences given. +# Sources of extrinsic information: M RM +# Initializing the parameters using config directory /opt/linux/centos/8.x/x86_64/pkgs/augustus/3.5.0/env/config/ ... +# pichia_stipitis version. Using default transition matrix. +# Looks like windows.fa is in fasta format. +# We have hints for 0 sequences and for 0 of the sequences in the input set. +# +# ----- prediction on sequence number 1 (length = 19001, name = pf_a_cp048715) ----- +# +# Predicted genes for sequence number 1 on both strands +# start gene g1 +pf_a_cp048715 AUGUSTUS gene 746 1603 0.5 - . ID=g1 +pf_a_cp048715 AUGUSTUS transcript 746 1603 0.5 - . ID=g1.t1;Parent=g1 +pf_a_cp048715 AUGUSTUS stop_codon 746 748 . - 0 Parent=g1.t1 +pf_a_cp048715 AUGUSTUS CDS 749 1603 0.5 - 0 ID=g1.t1.cds;Parent=g1.t1 +pf_a_cp048715 AUGUSTUS start_codon 1601 1603 . - 0 Parent=g1.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagaccagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcggaatacattagggcacaggatttcaagtatatcgagacgatttcagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# agaagagattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgatgaagatgac +# accagtgaagacacagagcgactcgtttctgacgctgctgatattatctggccaaagtcctatgattctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgacctcgctcgactattcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEDQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETISDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVKKRFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSDAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g1 +### +# start gene g2 +pf_a_cp048715 AUGUSTUS gene 1950 2876 0.65 - . ID=g2 +pf_a_cp048715 AUGUSTUS transcript 1950 2876 0.65 - . ID=g2.t1;Parent=g2 +pf_a_cp048715 AUGUSTUS stop_codon 1950 1952 . - 0 Parent=g2.t1 +pf_a_cp048715 AUGUSTUS CDS 1953 2876 0.65 - 0 ID=g2.t1.cds;Parent=g2.t1 +pf_a_cp048715 AUGUSTUS start_codon 2874 2876 . - 0 Parent=g2.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# cagcacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccagatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttgtctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggagtgctcatgacctcgtcacaggttgtcacctatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgagcaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaatactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVTARQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILSTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g2 +### +# start gene g3 +pf_a_cp048715 AUGUSTUS gene 3351 4364 0.23 - . ID=g3 +pf_a_cp048715 AUGUSTUS transcript 3351 4364 0.23 - . ID=g3.t1;Parent=g3 +pf_a_cp048715 AUGUSTUS stop_codon 3351 3353 . - 0 Parent=g3.t1 +pf_a_cp048715 AUGUSTUS intron 3921 3992 0.32 - . Parent=g3.t1 +pf_a_cp048715 AUGUSTUS CDS 3354 3920 0.25 - 0 ID=g3.t1.cds;Parent=g3.t1 +pf_a_cp048715 AUGUSTUS CDS 3993 4364 0.33 - 0 ID=g3.t1.cds;Parent=g3.t1 +pf_a_cp048715 AUGUSTUS start_codon 4362 4364 . - 0 Parent=g3.t1 +# coding sequence = [atggttactattggcgcccagtgctgcagaaacggagaagcacctccgtcgataagcacctttcccatcctctcgaaaa +# tatcattcaccctcaccaagacagacctaccaactaaagtcttttgggaaaagaagcagtttccagtggcgagccgactagctgctggagcaaacgct +# ccccgcagtcccaaagagattacttttgcccatctatcttccggaaccagcacaaaatcgatctatgtttacgctccacacaacaacgttgcacgtcc +# tcgtaataagttcatcattgccaggactatactgagccggattgtcaagaccagacacccttggtcgactaacgatgtctccaaaatcatatccaaaa +# tctggggctcaggaaatcgcttattcttgagctattttgactatctctcttttcttgaaaacgagtcacacgaatgtcgctatccactctatcattac +# aatccgcggtcccgaagcacagttgctaaagccagtgcttgtactgctagtgctaagaacagtgagttagaacagaacatgtcagttatctttttgaa +# cgatccggaaaactcaatcaagcagagtaagcaaaagaccgaaaaagcttcagctagtgatatcaaagatatgagcagttatgtcaagctttgcacta +# gtgtttatgaggaagacgactgcaaacggaggggggtcgatgctctccacgattcagatgactacaaagaccatgtgcttgtattaacgcttaacgag +# cctaatatgaaagctccggttgactctgctagtacaaacgccaggacaacatataaggtaacaaagcccacaaaaaagaagaagacgccgcctcattt +# ggagcttgtattcagtaaattcaggtcccctttgtatggtgaagccgaggatatttgcatcaacagttcccctatgtga] +# protein sequence = [MVTIGAQCCRNGEAPPSISTFPILSKISFTLTKTDLPTKVFWEKKQFPVASRLAAGANAPRSPKEITFAHLSSGTSTK +# SIYVYAPHNNVARPRNKFIIARTILSRIVKTRHPWSTNDVSKIISKIWGSGNRLFLSYFDYLSFLENESHECRYPLYHYNPRSRSTVAKASACTASAK +# NSELEQNMSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAPVDSASTNARTTYK +# VTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g3 +### +# start gene g4 +pf_a_cp048715 AUGUSTUS gene 5742 6971 0.94 - . ID=g4 +pf_a_cp048715 AUGUSTUS transcript 5742 6971 0.94 - . ID=g4.t1;Parent=g4 +pf_a_cp048715 AUGUSTUS stop_codon 5742 5744 . - 0 Parent=g4.t1 +pf_a_cp048715 AUGUSTUS CDS 5745 6971 0.94 - 0 ID=g4.t1.cds;Parent=g4.t1 +pf_a_cp048715 AUGUSTUS start_codon 6969 6971 . - 0 Parent=g4.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacgagacaagagctcgagaagacggtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcacaggaatcaccgacgactcccagacatccaaacta +# cagctcatctactatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggctcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaactag] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLTGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g4 +### +# start gene g5 +pf_a_cp048715 AUGUSTUS gene 7276 7797 1 + . ID=g5 +pf_a_cp048715 AUGUSTUS transcript 7276 7797 1 + . ID=g5.t1;Parent=g5 +pf_a_cp048715 AUGUSTUS start_codon 7276 7278 . + 0 Parent=g5.t1 +pf_a_cp048715 AUGUSTUS CDS 7276 7794 1 + 0 ID=g5.t1.cds;Parent=g5.t1 +pf_a_cp048715 AUGUSTUS stop_codon 7795 7797 . + 0 Parent=g5.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g5 +### +# start gene g6 +pf_a_cp048715 AUGUSTUS gene 8035 9804 1 - . ID=g6 +pf_a_cp048715 AUGUSTUS transcript 8035 9804 1 - . ID=g6.t1;Parent=g6 +pf_a_cp048715 AUGUSTUS stop_codon 8035 8037 . - 0 Parent=g6.t1 +pf_a_cp048715 AUGUSTUS CDS 8038 9804 1 - 0 ID=g6.t1.cds;Parent=g6.t1 +pf_a_cp048715 AUGUSTUS start_codon 9802 9804 . - 0 Parent=g6.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccgggacagcacgctgatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtcgtttcggcacatcctgaacatcaccaccaggagacttcatccaaggacgagcag +# ctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccatttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaatcttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatcagtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtt +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gatactgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgaggtccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaacgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgtgcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgagggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g6 +### +# start gene g7 +pf_a_cp048715 AUGUSTUS gene 9942 11175 0.5 + . ID=g7 +pf_a_cp048715 AUGUSTUS transcript 9942 11175 0.5 + . ID=g7.t1;Parent=g7 +pf_a_cp048715 AUGUSTUS start_codon 9942 9944 . + 0 Parent=g7.t1 +pf_a_cp048715 AUGUSTUS intron 10036 10390 0.5 + . Parent=g7.t1 +pf_a_cp048715 AUGUSTUS CDS 9942 10035 0.55 + 0 ID=g7.t1.cds;Parent=g7.t1 +pf_a_cp048715 AUGUSTUS CDS 10391 11172 0.8 + 2 ID=g7.t1.cds;Parent=g7.t1 +pf_a_cp048715 AUGUSTUS stop_codon 11173 11175 . + 0 Parent=g7.t1 +# coding sequence = [atgcagtcattagacacacaggcttccagtcgaggctacgcgagcattggtggtcctcacgagaaacagaaagggatag +# gctgtgctgtatgctcttcagtgtcacttgagagcatcaaggccggtcttctgctcgctcgtaacccaatcgtcaccccagatctcacacctctcgaa +# aatgcatacttcaactaccaggaggaacttgaacgcagactgatgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcg +# tttcttcaaggcacagcgtggacctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcga +# agcaggaggttgtgattccaaaggatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagac +# gaacagggtgatttgacaagtttggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcga +# cgttacagagggtacaccattgcacagagtcgccgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaa +# cggcagtcttaaagtttgatggctgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctca +# ggtgcattcaagccacagaaggttgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagttttt +# gttctctcgtgtctga] +# protein sequence = [MQSLDTQASSRGYASIGGPHEKQKGIGCAVCSSVSLESIKAGLLLARNPIVTPDLTPLENAYFNYQEELERRLMWTFP +# HYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNSRVTKADEQGDLTSLERQLPRTLYLL +# VKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFIIKSNILSGAFKPQKVEYAWLTREEIE +# EKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 1 +# RM: 1 +# incompatible hint groups: 3 +# RM: 3 +# end gene g7 +### +# start gene g8 +pf_a_cp048715 AUGUSTUS gene 11319 12716 0.87 - . ID=g8 +pf_a_cp048715 AUGUSTUS transcript 11319 12716 0.87 - . ID=g8.t1;Parent=g8 +pf_a_cp048715 AUGUSTUS stop_codon 11319 11321 . - 0 Parent=g8.t1 +pf_a_cp048715 AUGUSTUS CDS 11322 12716 0.87 - 0 ID=g8.t1.cds;Parent=g8.t1 +pf_a_cp048715 AUGUSTUS start_codon 12714 12716 . - 0 Parent=g8.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggtgacgctcgaggaagtggtagaggagaaggaagaggtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaatgtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatttttgtcggtcttgtga +# ccggtaagatctctgttgttatcaagggcggtgatggtgcctttgccaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggcatcatctcactctggtccaccacagacctatcattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccatcttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRGNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHECMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFANACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLSLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g8 +### +# start gene g9 +pf_a_cp048715 AUGUSTUS gene 13491 14789 0.88 + . ID=g9 +pf_a_cp048715 AUGUSTUS transcript 13491 14789 0.88 + . ID=g9.t1;Parent=g9 +pf_a_cp048715 AUGUSTUS start_codon 13491 13493 . + 0 Parent=g9.t1 +pf_a_cp048715 AUGUSTUS CDS 13491 14786 0.88 + 0 ID=g9.t1.cds;Parent=g9.t1 +pf_a_cp048715 AUGUSTUS stop_codon 14787 14789 . + 0 Parent=g9.t1 +# coding sequence = [atgtctccaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcgatcagacgctgacttcatgaact +# ccattcgtgctccaaggatccccagtcgagctgctcaagctgactacgagaagcacactgctagcgctcgtctacaacataaacgtgaggatacagag +# gtcaagggaatggatcaacaattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggc +# gctgattaaaggtgtgaaatcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagt +# cagggagtaagtcacctcaccgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccggggccgtcatgaaggttca +# gtgggggacgctctaggctcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaagga +# tgatgaaagtcataaagctatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatc +# ttgagatgatgaagcgcaggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaat +# gagtttgaagaggttgacgagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagtttccgggaacattaaccct +# cggtgagattgagcaaaaggtcaaccagcacatggactgcattccaaaaattctcaacggtcgtcttgactccacttacctgcaaaaagcgaaagtat +# tcatgcacgactctggagcaacaatcttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaaaagacaa +# ggggctattgctaatctaatcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacatt +# cacattacacattctcgctccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacag +# ttgattatggtcaatacatcgctgatttggtcccttgtgaatga] +# protein sequence = [MSPGEEEQEGSRHRIPSIRSDADFMNSIRAPRIPSRAAQADYEKHTASARLQHKREDTEVKGMDQQLHDDTNSGKRET +# KKGEDVAPIPRKRALIKGVKSSAKKSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRGRHEGSVGDALGSDEELFNFDSPGP +# SSQELRSAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVK +# RKYGNEEFPGTLTLGEIEQKVNQHMDCIPKILNGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKA +# EHNRVIQFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 6 +# RM: 6 +# end gene g9 +### +# start gene g10 +pf_a_cp048715 AUGUSTUS gene 15247 18372 0.99 + . ID=g10 +pf_a_cp048715 AUGUSTUS transcript 15247 18372 0.99 + . ID=g10.t1;Parent=g10 +pf_a_cp048715 AUGUSTUS start_codon 15247 15249 . + 0 Parent=g10.t1 +pf_a_cp048715 AUGUSTUS CDS 15247 18369 0.99 + 0 ID=g10.t1.cds;Parent=g10.t1 +pf_a_cp048715 AUGUSTUS stop_codon 18370 18372 . + 0 Parent=g10.t1 +# coding sequence = [atgtcgaagacaatctccgactccgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctatggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgccgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctctaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagttagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaaaaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaaaaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacctgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacatcaatggtgatcttggtgatgcagttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctattgccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgaccaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcttccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagtgaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacgtctgcctgcagatctttggtcaacca +# agttcaggatattttgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagatcagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAIAVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 18 +# RM: 18 +# end gene g10 +### +# +# ----- prediction on sequence number 2 (length = 19501, name = pf_a_jcm3601) ----- +# +# Predicted genes for sequence number 2 on both strands +# start gene g11 +pf_a_jcm3601 AUGUSTUS gene 944 3074 0.31 - . ID=g11 +pf_a_jcm3601 AUGUSTUS transcript 944 3074 0.31 - . ID=g11.t1;Parent=g11 +pf_a_jcm3601 AUGUSTUS stop_codon 944 946 . - 0 Parent=g11.t1 +pf_a_jcm3601 AUGUSTUS intron 1594 2155 0.42 - . Parent=g11.t1 +pf_a_jcm3601 AUGUSTUS CDS 947 1593 0.42 - 2 ID=g11.t1.cds;Parent=g11.t1 +pf_a_jcm3601 AUGUSTUS CDS 2156 3074 0.66 - 0 ID=g11.t1.cds;Parent=g11.t1 +pf_a_jcm3601 AUGUSTUS start_codon 3072 3074 . - 0 Parent=g11.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtgg +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttttctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggttgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctggt +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttgaatacattagggcacaggatttcaagtatatcgagacgattt +# tagatatttatgtctcttgggatgctgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagat +# ggtgatttccccgcacagattgtgacgaagaaattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgc +# aaacttgagtgatgacgaagatgacaccagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgaatctatca +# atccatccaagatcacagagaagaagacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgc +# aagccgggcaaggaattcccgaacggtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagag +# ggatggtctcgaggaggaagttgactctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatg +# aagatgaagatgagatgtaa] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVAPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVEYIRAQDFKYIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDP +# EELAAAASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPKSYESINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADD +# LFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKRKRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 3 +# RM: 3 +# incompatible hint groups: 10 +# RM: 10 +# end gene g11 +### +# start gene g12 +pf_a_jcm3601 AUGUSTUS gene 3549 4562 0.41 - . ID=g12 +pf_a_jcm3601 AUGUSTUS transcript 3549 4562 0.41 - . ID=g12.t1;Parent=g12 +pf_a_jcm3601 AUGUSTUS stop_codon 3549 3551 . - 0 Parent=g12.t1 +pf_a_jcm3601 AUGUSTUS intron 4119 4190 0.59 - . Parent=g12.t1 +pf_a_jcm3601 AUGUSTUS CDS 3552 4118 0.48 - 0 ID=g12.t1.cds;Parent=g12.t1 +pf_a_jcm3601 AUGUSTUS CDS 4191 4562 0.54 - 0 ID=g12.t1.cds;Parent=g12.t1 +pf_a_jcm3601 AUGUSTUS start_codon 4560 4562 . - 0 Parent=g12.t1 +# coding sequence = [atggttactattggcgcccagtgctgcagaaacggagaagcacctccgtcgataagcacctttcccatcctctcgaaaa +# tatcattcaccctcaccaagacagatctaccaactaaagtcttttgggaaaagaagcagtttccagtggcgagcccactagctgctggagcaaacgct +# ccccgcagtcccaaagagattacttttgcccatctatcttccggaaccagcacaaaatcgatctatgtttatgctccacacaacaacgttgcacgtcc +# tcgtaataagttcatcattgccaggactatactgagccggattgtcaagaccagacacccttggtcgactaacgatgtctccaaaatcatatccaaaa +# tctggggctcaggaaatcgcttattcttgagctattttgactatctctcctttcttgaaaacgagtcacacgaatgtcgctatccactctaccattac +# aatccgcggtcccgaagcacagttgctaaagccagtgcttgtactgctagtgctaagaacagtgagttagaacagaacatgtcagttatctttttgaa +# cgatccggaaaactcaatcaagcagagtaagcaaaagaccgaaaaagcttcagctagtgatatcaaagatatgagcagttatgtcaagctttgcacta +# gtgtttatgaggaagacgactgcaaacggaggggggtcgatgctctccacgattcagatgactacaaagaccatgtgcttgtattaacgcttaacgag +# cctaatatgaaagctccggttggctctgctagtacaaacgccaggacaacatataaggtaacaaagcccacaaaaaagaagaagacgccgcctcattt +# ggagcttgtattcagtaaattcaggtcccctttgtatggtgaagccgaggatatttgcatcaacagttcccctatgtga] +# protein sequence = [MVTIGAQCCRNGEAPPSISTFPILSKISFTLTKTDLPTKVFWEKKQFPVASPLAAGANAPRSPKEITFAHLSSGTSTK +# SIYVYAPHNNVARPRNKFIIARTILSRIVKTRHPWSTNDVSKIISKIWGSGNRLFLSYFDYLSFLENESHECRYPLYHYNPRSRSTVAKASACTASAK +# NSELEQNMSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAPVGSASTNARTTYK +# VTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g12 +### +# start gene g13 +pf_a_jcm3601 AUGUSTUS gene 5937 7166 0.96 - . ID=g13 +pf_a_jcm3601 AUGUSTUS transcript 5937 7166 0.96 - . ID=g13.t1;Parent=g13 +pf_a_jcm3601 AUGUSTUS stop_codon 5937 5939 . - 0 Parent=g13.t1 +pf_a_jcm3601 AUGUSTUS CDS 5940 7166 0.96 - 0 ID=g13.t1.cds;Parent=g13.t1 +pf_a_jcm3601 AUGUSTUS start_codon 7164 7166 . - 0 Parent=g13.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctactatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggctcttggtgctattgtcaaaagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaactag] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g13 +### +# start gene g14 +pf_a_jcm3601 AUGUSTUS gene 7472 7993 1 + . ID=g14 +pf_a_jcm3601 AUGUSTUS transcript 7472 7993 1 + . ID=g14.t1;Parent=g14 +pf_a_jcm3601 AUGUSTUS start_codon 7472 7474 . + 0 Parent=g14.t1 +pf_a_jcm3601 AUGUSTUS CDS 7472 7990 1 + 0 ID=g14.t1.cds;Parent=g14.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 7991 7993 . + 0 Parent=g14.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgaaatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g14 +### +# start gene g15 +pf_a_jcm3601 AUGUSTUS gene 8231 10000 1 - . ID=g15 +pf_a_jcm3601 AUGUSTUS transcript 8231 10000 1 - . ID=g15.t1;Parent=g15 +pf_a_jcm3601 AUGUSTUS stop_codon 8231 8233 . - 0 Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS CDS 8234 10000 1 - 0 ID=g15.t1.cds;Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS start_codon 9998 10000 . - 0 Parent=g15.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggacagcacgctgatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcacgaccaggagacttcatccaaggacgagcag +# ctggagaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccatttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaggcatttacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtc +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcaaacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaatgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgtgcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgagggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHDQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g15 +### +# start gene g16 +pf_a_jcm3601 AUGUSTUS gene 10456 11370 0.5 + . ID=g16 +pf_a_jcm3601 AUGUSTUS transcript 10456 11370 0.5 + . ID=g16.t1;Parent=g16 +pf_a_jcm3601 AUGUSTUS start_codon 10456 10458 . + 0 Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS intron 10478 10591 0.5 + . Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS CDS 10456 10477 0.52 + 0 ID=g16.t1.cds;Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS CDS 10592 11367 0.62 + 2 ID=g16.t1.cds;Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 11368 11370 . + 0 Parent=g16.t1 +# coding sequence = [atgatgcagaatggtcttcagctgtcacttgagagcatcaaggccggtcttctgctcgctcgtaacccaatcgtcaccc +# cagatctcacacctctcgaaaatgcatactttaactaccaggaggaactcgaacgcagactgatgtggacattcccacactactactacttcaagaag +# ggttccctcgccgaacgtcgtttcttcaaggcacagcgtggacctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgca +# caaccgtgaacgtcgctcgaagcaggaggttgtgattccaaaggatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaact +# ctcgtgttacaaaagcagacgaacagggtgatttgacaagtttggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggc +# tggaagctgccaagtttcgacgttacagagggtacaccattgcacagagtcgccgaggagggtttaagacaacttggtggtgaccagattaacacatg +# gactgtcgctcgttctccaacggcagtcttgaagtttgatggctgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatca +# ttaagtccaatatcctctcaggtgcattcaagccacagaaggttgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatac +# ttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MMQNGLQLSLESIKAGLLLARNPIVTPDLTPLENAYFNYQEELERRLMWTFPHYYYFKKGSLAERRFFKAQRGPVSKQ +# PGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNSRVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAE +# EGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFIIKSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 1 +# RM: 1 +# incompatible hint groups: 2 +# RM: 2 +# end gene g16 +### +# start gene g17 +pf_a_jcm3601 AUGUSTUS gene 11514 12911 1 - . ID=g17 +pf_a_jcm3601 AUGUSTUS transcript 11514 12911 1 - . ID=g17.t1;Parent=g17 +pf_a_jcm3601 AUGUSTUS stop_codon 11514 11516 . - 0 Parent=g17.t1 +pf_a_jcm3601 AUGUSTUS CDS 11517 12911 1 - 0 ID=g17.t1.cds;Parent=g17.t1 +pf_a_jcm3601 AUGUSTUS start_codon 12909 12911 . - 0 Parent=g17.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggcgacgctcgaggaagtggtagaggggaaggaagaagtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatatttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccaccttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRSNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g17 +### +# start gene g18 +pf_a_jcm3601 AUGUSTUS gene 13686 14984 0.83 + . ID=g18 +pf_a_jcm3601 AUGUSTUS transcript 13686 14984 0.83 + . ID=g18.t1;Parent=g18 +pf_a_jcm3601 AUGUSTUS start_codon 13686 13688 . + 0 Parent=g18.t1 +pf_a_jcm3601 AUGUSTUS CDS 13686 14981 0.83 + 0 ID=g18.t1.cds;Parent=g18.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 14982 14984 . + 0 Parent=g18.t1 +# coding sequence = [atgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaact +# ccattcgtgctccaaggattcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagag +# gtcaaggaaatggatcaacaattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggc +# gctgattaaaggtgtgaaatcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagt +# cagggagtaagtcacctcaccgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttca +# gtgggggacgctctaggctcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaagga +# tgatgaaagtcataaagctatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatc +# ttgagatgatgaaacgcaggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaat +# gagtttgaagaggttgacgagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacattaagcct +# cggtgagattgagcaaaaggccaaccagcacatggactgcattccaaaaattctcgacggtcgtcttgactccacttacctacaaaaagcgaaagtat +# tcatgcacgactctggagcaacaattttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaa +# ggggctattgccaatctgatcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacatt +# cacattacacattctcgctccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacag +# ttgattatggtcaatacatcgctgatttggtcccttgtgaatga] +# protein sequence = [MSSGEEEQEGSRHRIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRET +# KKGEDVAPIPRKRALIKGVKSSAKKSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGP +# SSQELRSAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVK +# RKYGNEEFPGTLSLGEIEQKANQHMDCIPKILDGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKA +# EHNRVIQFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 6 +# RM: 6 +# end gene g18 +### +# start gene g19 +pf_a_jcm3601 AUGUSTUS gene 15442 18567 1 + . ID=g19 +pf_a_jcm3601 AUGUSTUS transcript 15442 18567 1 + . ID=g19.t1;Parent=g19 +pf_a_jcm3601 AUGUSTUS start_codon 15442 15444 . + 0 Parent=g19.t1 +pf_a_jcm3601 AUGUSTUS CDS 15442 18564 1 + 0 ID=g19.t1.cds;Parent=g19.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 18565 18567 . + 0 Parent=g19.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatatggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagcagctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatgttgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDVDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g19 +### +# +# ----- prediction on sequence number 3 (length = 22001, name = pf_alpha_strain65) ----- +# +# Predicted genes for sequence number 3 on both strands +# start gene g20 +pf_alpha_strain65 AUGUSTUS gene 704 1561 0.37 - . ID=g20 +pf_alpha_strain65 AUGUSTUS transcript 704 1561 0.37 - . ID=g20.t1;Parent=g20 +pf_alpha_strain65 AUGUSTUS stop_codon 704 706 . - 0 Parent=g20.t1 +pf_alpha_strain65 AUGUSTUS CDS 707 1561 0.37 - 0 ID=g20.t1.cds;Parent=g20.t1 +pf_alpha_strain65 AUGUSTUS start_codon 1559 1561 . - 0 Parent=g20.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgatgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgactctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g20 +### +# start gene g21 +pf_alpha_strain65 AUGUSTUS gene 1908 2834 0.49 - . ID=g21 +pf_alpha_strain65 AUGUSTUS transcript 1908 2834 0.49 - . ID=g21.t1;Parent=g21 +pf_alpha_strain65 AUGUSTUS stop_codon 1908 1910 . - 0 Parent=g21.t1 +pf_alpha_strain65 AUGUSTUS CDS 1911 2834 0.49 - 0 ID=g21.t1.cds;Parent=g21.t1 +pf_alpha_strain65 AUGUSTUS start_codon 2832 2834 . - 0 Parent=g21.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttttctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g21 +### +# start gene g22 +pf_alpha_strain65 AUGUSTUS gene 3495 5891 0.42 + . ID=g22 +pf_alpha_strain65 AUGUSTUS transcript 3495 5891 0.42 + . ID=g22.t1;Parent=g22 +pf_alpha_strain65 AUGUSTUS start_codon 3495 3497 . + 0 Parent=g22.t1 +pf_alpha_strain65 AUGUSTUS CDS 3495 5888 0.42 + 0 ID=g22.t1.cds;Parent=g22.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 5889 5891 . + 0 Parent=g22.t1 +# coding sequence = [atgttgtcccaccgccatcagaaagctgctctctccaaactgcaaagaagtgcttacgcacattacccaacaccacatt +# cgccaatatctctaacaccaaatagatcaatatcgtctcttccggcctcgaagatgcttccagctgtcaattacgttcgccaagcagatccagatgcg +# attgttcttctcggtcatggtgaatcttaccatgctttcggcagagtcgatactaatctgattaaaggaatggtatcagctggcaatgcaagcgagat +# actctaccgtgatgatggactgacacaatggagattcaagtcatctcgactcccgcataacacttcttggctggtgaaggagttgaacaggccagttg +# tcaccttggaatggaacgaatatgctactagtttcctacagccattgcgcaacgactcccattggcatttcctatcgagaaccattcctgatggccac +# tcagaccatgaattctctgttcattctgaatctcaaaacatcatagtaattggtatacaggggaagattctcaaggggatcaaaacaggtgaaaagtc +# acagagacgtgttggtatcacttgctttgacccaacaaacggatcgatgtctgttcagggcacttctcttgctgatttgagatcccatatcgtgagct +# ttcgtcctcgggctattgttctggactccagtctgattgggacataccgggacatcattggcacagctcttctaggttcaccagaagttaagtttacc +# aatatgcctcccgtgttgggctcgctacaactccttaatgatttctttgatgtctctggtgaaatttggaggaagtgtttcgatgattacgagctgta +# ctcactgaaggctgctgtggagttcattaagaagacacagaaatcaacgtctgcagtattccaactaccatcgagggaactgtcgtcacaatggaccg +# tgttattggacaaagaaactatcagttccttgaacctgggctccaccgggagtcactatatgaattcctcctggttacttcaaagatcaatcaagact +# gctactgttggtggttctcgaatgttgtacaagtctatcttgaggccaactaggatagtcagtgagattttgtttcgtcagagggcggttcaatccat +# cattaaaaggaaacatacacgtcagaagctgagctcagagctctccagtttaccagacatcccattgctactacagaaactcaagatctatccggaat +# cagaagaccttgtcagtactatactacactccatcgacatcatctcgtcacttgcccccatcgcctccaagttttacatgaacggtcaccagcaagac +# gctgactttgatgatggtgacttgatttaccgcattccatctgcaaaacgtttggaggaactcagagacagggcccgttcccttctcaatgctagcga +# cacaatgcatggtgttgtttcctcgaatacaagcattgaatgcaatgatcggaggacacagaaagctactttggtgaatggtgttgattgtggaccta +# ataggaacacttctttttctcactctgtgaaaaatcagacaggtgatgtttctttggaaacctttatggtcagcgatgatgctgaattgaacactgag +# tcaccttctaatctcacaacagtgctagacacccgtactgtgagtgaacacacccagcctaaggggctaggagagattaccagctcatctttggacta +# ccgagatctacatgctacacaccgcatcgacaagttggccggtcagataagtgaattacaccctggccaccctctaccaagagccaaagtgattgcca +# actgctcggctgaaatattaagacataaggagctcattctactcttctcccgactcttgtcacgtttggacatgctgctgtcgtttgccaacgtggct +# atccaacatgacgacacaagacctatcgtggatcccagcagaaccaactttgagattcacttgaaggacaaagatttcaatgctgctgctgatccatt +# gttgatgtcaaactcatcatcagtattgtggactaagggagagagaaaaaccgtggcagagctttacgggctgtacgccagtatcatcttattggcgc +# actcaggtgcttatgttccagccaagtctgctaacattggtgttgtcagccacatgctttgtcaactccgctctacaaatcctttcctattacattca +# gcgcttgagaagtctatggatggccgtcgtatcatcatgtgcgacgctctgcctgacgatttcgaagactatggctcagccgatgacagattggaaag +# ctaccaagaaacactggcgactatgaatgtgggaactaaccgactgcttctaagatatcactga] +# protein sequence = [MLSHRHQKAALSKLQRSAYAHYPTPHSPISLTPNRSISSLPASKMLPAVNYVRQADPDAIVLLGHGESYHAFGRVDTN +# LIKGMVSAGNASEILYRDDGLTQWRFKSSRLPHNTSWLVKELNRPVVTLEWNEYATSFLQPLRNDSHWHFLSRTIPDGHSDHEFSVHSESQNIIVIGI +# QGKILKGIKTGEKSQRRVGITCFDPTNGSMSVQGTSLADLRSHIVSFRPRAIVLDSSLIGTYRDIIGTALLGSPEVKFTNMPPVLGSLQLLNDFFDVS +# GEIWRKCFDDYELYSLKAAVEFIKKTQKSTSAVFQLPSRELSSQWTVLLDKETISSLNLGSTGSHYMNSSWLLQRSIKTATVGGSRMLYKSILRPTRI +# VSEILFRQRAVQSIIKRKHTRQKLSSELSSLPDIPLLLQKLKIYPESEDLVSTILHSIDIISSLAPIASKFYMNGHQQDADFDDGDLIYRIPSAKRLE +# ELRDRARSLLNASDTMHGVVSSNTSIECNDRRTQKATLVNGVDCGPNRNTSFSHSVKNQTGDVSLETFMVSDDAELNTESPSNLTTVLDTRTVSEHTQ +# PKGLGEITSSSLDYRDLHATHRIDKLAGQISELHPGHPLPRAKVIANCSAEILRHKELILLFSRLLSRLDMLLSFANVAIQHDDTRPIVDPSRTNFEI +# HLKDKDFNAAADPLLMSNSSSVLWTKGERKTVAELYGLYASIILLAHSGAYVPAKSANIGVVSHMLCQLRSTNPFLLHSALEKSMDGRRIIMCDALPD +# DFEDYGSADDRLESYQETLATMNVGTNRLLLRYH] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g22 +### +# start gene g23 +pf_alpha_strain65 AUGUSTUS gene 8788 10017 0.96 - . ID=g23 +pf_alpha_strain65 AUGUSTUS transcript 8788 10017 0.96 - . ID=g23.t1;Parent=g23 +pf_alpha_strain65 AUGUSTUS stop_codon 8788 8790 . - 0 Parent=g23.t1 +pf_alpha_strain65 AUGUSTUS CDS 8791 10017 0.96 - 0 ID=g23.t1.cds;Parent=g23.t1 +pf_alpha_strain65 AUGUSTUS start_codon 10015 10017 . - 0 Parent=g23.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacatacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctattatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggcgcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaattga] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g23 +### +# start gene g24 +pf_alpha_strain65 AUGUSTUS gene 10323 10844 1 + . ID=g24 +pf_alpha_strain65 AUGUSTUS transcript 10323 10844 1 + . ID=g24.t1;Parent=g24 +pf_alpha_strain65 AUGUSTUS start_codon 10323 10325 . + 0 Parent=g24.t1 +pf_alpha_strain65 AUGUSTUS CDS 10323 10841 1 + 0 ID=g24.t1.cds;Parent=g24.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 10842 10844 . + 0 Parent=g24.t1 +# coding sequence = [atgaacagcataacccaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g24 +### +# start gene g25 +pf_alpha_strain65 AUGUSTUS gene 11081 12850 1 - . ID=g25 +pf_alpha_strain65 AUGUSTUS transcript 11081 12850 1 - . ID=g25.t1;Parent=g25 +pf_alpha_strain65 AUGUSTUS stop_codon 11081 11083 . - 0 Parent=g25.t1 +pf_alpha_strain65 AUGUSTUS CDS 11084 12850 1 - 0 ID=g25.t1.cds;Parent=g25.t1 +pf_alpha_strain65 AUGUSTUS start_codon 12848 12850 . - 0 Parent=g25.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggacagcacgctgatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcaccaccaggagacttcatccaaggacgagcag +# ctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccgtttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtc +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaatgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgttcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgaaggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g25 +### +# start gene g26 +pf_alpha_strain65 AUGUSTUS gene 12988 14224 0.55 + . ID=g26 +pf_alpha_strain65 AUGUSTUS transcript 12988 14224 0.55 + . ID=g26.t1;Parent=g26 +pf_alpha_strain65 AUGUSTUS start_codon 12988 12990 . + 0 Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS intron 13082 13445 0.55 + . Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS CDS 12988 13081 0.61 + 0 ID=g26.t1.cds;Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS CDS 13446 14221 0.75 + 2 ID=g26.t1.cds;Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 14222 14224 . + 0 Parent=g26.t1 +# coding sequence = [atgcagtcattagacacacaggcttccagtcgaggctacgcgagcattggtggtcctgacgagaaacagaaagggatag +# gctgtgctgtatgcttgtcacttgagagcatcaaggccggtcttctgctcgctcgtaacccaatcgtcaccccagatctcacacctctcgaaaatgca +# tacttcaactaccaggaggaactcgaacgcagactgatgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttctt +# caaggcacagcgtggacctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcagg +# aggttgtgattccaaaggatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacag +# ggtgatttgacaagtttggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcgacgttac +# agagggtacaccattgcacagagtcgctgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcag +# tcttaaagtttgatggctgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgca +# ttcaagccacagaaggttgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctc +# tcgtgtctga] +# protein sequence = [MQSLDTQASSRGYASIGGPDEKQKGIGCAVCLSLESIKAGLLLARNPIVTPDLTPLENAYFNYQEELERRLMWTFPHY +# YYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNSRVTKADEQGDLTSLERQLPRTLYLLVK +# DGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFIIKSNILSGAFKPQKVEYAWLTREEIEEK +# VSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 1 +# RM: 1 +# incompatible hint groups: 2 +# RM: 2 +# end gene g26 +### +# start gene g27 +pf_alpha_strain65 AUGUSTUS gene 14368 15765 0.77 - . ID=g27 +pf_alpha_strain65 AUGUSTUS transcript 14368 15765 0.77 - . ID=g27.t1;Parent=g27 +pf_alpha_strain65 AUGUSTUS stop_codon 14368 14370 . - 0 Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS intron 15358 15468 0.79 - . Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS CDS 14371 15357 0.78 - 0 ID=g27.t1.cds;Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS CDS 15469 15765 0.78 - 0 ID=g27.t1.cds;Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS start_codon 15763 15765 . - 0 Parent=g27.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggcgacgctcgaggaagtggtagaggggaaggaagaagtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtcaaaccaaatcgtg +# aaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatactcgttatatggaac +# gtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcatggagaccagaaag +# cgatgagattttagccacaattggaaaggacaggagcattcatatttggaacattgtgactggtaagctaatgcatgagattgagaccgcagagacaa +# caccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaacacatacgaacag +# ttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatttttgtcggtcttgtgactggtaagatctc +# tgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatggacccacggggtc +# gctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgttgatcagtcaatt +# catagaatggatctctttgctgatggcgtccatcttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagagaccggtaaagaggt +# cgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacacaaatggtgatgggt +# ctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRSNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSYNGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKL +# HEGMVTRIAWRPESDEILATIGKDRSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLAVITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNN +# SVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLWSTTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEA +# DSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g27 +### +# start gene g28 +pf_alpha_strain65 AUGUSTUS gene 16540 17838 0.81 + . ID=g28 +pf_alpha_strain65 AUGUSTUS transcript 16540 17838 0.81 + . ID=g28.t1;Parent=g28 +pf_alpha_strain65 AUGUSTUS start_codon 16540 16542 . + 0 Parent=g28.t1 +pf_alpha_strain65 AUGUSTUS CDS 16540 17835 0.81 + 0 ID=g28.t1.cds;Parent=g28.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 17836 17838 . + 0 Parent=g28.t1 +# coding sequence = [atgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaact +# ccattcgtgctccaaggattcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagag +# gtcaaggaaatggatcaacaattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggc +# gctgattaaaggtgtgaaatcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagt +# cagggagtaagtcacctcaccgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttca +# gtgggggacgctctaggctcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaagga +# tgatgaaagtcataaagctatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatc +# ttgagatgatgaagcgcaggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaat +# gagtttgaagaggttgacgagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacactaagcct +# cggtgagattgagcaaaaggccaaccagcacatggactgcattccaaaaattctcgacggtcgtcttgactccacttacctacaaaaagcgaaagtat +# tcatgcacgactctggagcaacaattttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaa +# ggggctattgccaatctgatcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacatt +# cacattacacattctcgctccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacag +# ttgattatggtcaatacatcgctgatttggtcccttgtgaatga] +# protein sequence = [MSSGEEEQEGSRHRIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRET +# KKGEDVAPIPRKRALIKGVKSSAKKSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGP +# SSQELRSAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVK +# RKYGNEEFPGTLSLGEIEQKANQHMDCIPKILDGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKA +# EHNRVIQFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 6 +# RM: 6 +# end gene g28 +### +# start gene g29 +pf_alpha_strain65 AUGUSTUS gene 18296 21421 0.98 + . ID=g29 +pf_alpha_strain65 AUGUSTUS transcript 18296 21421 0.98 + . ID=g29.t1;Parent=g29 +pf_alpha_strain65 AUGUSTUS start_codon 18296 18298 . + 0 Parent=g29.t1 +pf_alpha_strain65 AUGUSTUS CDS 18296 21418 0.98 + 0 ID=g29.t1.cds;Parent=g29.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 21419 21421 . + 0 Parent=g29.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcagaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaccgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaga +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacctcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYETDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g29 +### +# +# ----- prediction on sequence number 4 (length = 22501, name = pf_alpha_tbrc4498) ----- +# +# Predicted genes for sequence number 4 on both strands +# start gene g30 +pf_alpha_tbrc4498 AUGUSTUS gene 943 1800 0.39 - . ID=g30 +pf_alpha_tbrc4498 AUGUSTUS transcript 943 1800 0.39 - . ID=g30.t1;Parent=g30 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 943 945 . - 0 Parent=g30.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 946 1800 0.39 - 0 ID=g30.t1.cds;Parent=g30.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 1798 1800 . - 0 Parent=g30.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaaccgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccactgcaagcaagaagaagtcgagtgcaaacttgagtgatgacgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgactctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAATASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g30 +### +# start gene g31 +pf_alpha_tbrc4498 AUGUSTUS gene 2147 3073 0.53 - . ID=g31 +pf_alpha_tbrc4498 AUGUSTUS transcript 2147 3073 0.53 - . ID=g31.t1;Parent=g31 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 2147 2149 . - 0 Parent=g31.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 2150 3073 0.53 - 0 ID=g31.t1.cds;Parent=g31.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 3071 3073 . - 0 Parent=g31.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccttcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttgtctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggcctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTFSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g31 +### +# start gene g32 +pf_alpha_tbrc4498 AUGUSTUS gene 3734 6130 0.38 + . ID=g32 +pf_alpha_tbrc4498 AUGUSTUS transcript 3734 6130 0.38 + . ID=g32.t1;Parent=g32 +pf_alpha_tbrc4498 AUGUSTUS start_codon 3734 3736 . + 0 Parent=g32.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 3734 6127 0.38 + 0 ID=g32.t1.cds;Parent=g32.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 6128 6130 . + 0 Parent=g32.t1 +# coding sequence = [atgttgtcccaccgccatcagaaagctgctctctccaaactgcaaagaagtgcttacgcacattacccaacaccacatt +# cgccaatatctctaacaccaaatagatcaatatcgtctcttccggcctcgaagatgcttccagctgtcaattacgttcgccaagcagatccagatgcg +# attgttcttctcggtcatggtgaatcttaccatgctttcggcagagtcgatactaatctgattaaaggaatggtatcagctggcaatgcaagcgagat +# actctaccgtgatgatggactgacacaatggagattcaagtcatctcgactcccgcataacacttcttggctggtgaaggagttgaacaggccagttg +# tcaccttggaatggaacgaatatgctactagtttcctacagccattgcgcaacgactcccattggcatttcctatcgagaaccattcctgatggccac +# tcagaccatgaattctctgttcattctgaatctcaaaacatcatagtaattggtatacaggggaagattctcaaggggatcaaaacaggtgaaaagtc +# acagagacgtgttggtatcacttgctttgacccaacaaacggatcgatgtctgttcagggcacttctcttgctgatttgagatcccatatcgtgagct +# ttcgtcctcgggctattgttctggactccagtctgattgggacataccgggacatcattggcacagctcttctaggttcaccagaagttaagtttacc +# aatatgcctcccgtgttgggctcgctacaactccttaatgatttctttgatgtctctggtgaaatttggaggaagtgtttcgatgattacgagctgta +# ctcactgaaggctgctgtggagttcattaagaagacacagaaatcaacgtctgcagtattccaactaccatcgagggaactgtcgtcacaatggaccg +# tgttattggacaaagaaactatcagttccttgaacctgggctccaccgggagtcactatatgaattcctcctggttacttcaaagatcaatcaagact +# gctactgttggtggttctcgaatgttgtacaagtctatcttgaggccaactaggatagtcagtgagattttgtttcgtcagagggcggttcaatccat +# cattaaaaggaaacatacacgtcagaagctgagctcagagctctcgagtttaccagacatcccattgctactacagaaactcaagatctatccggaat +# cagaagaccttgtcagtactatactacactccatcgacatcatctcgtcacttgctcccatcgcctccaagttttacatgaacggtcgccagcaagac +# gctgactttgatgatggtgacttgatttaccgcattccatctgcaaaacgtttggaggaactcagagacagggcccgttcccttctcaatgctagcga +# cacaatgcatggtgttgtttcctcgaatacaagcattgaatgcaatgatcggaggacacagaaagctactttggtgaatggtgttgattgtggaccta +# ataggaacacttctttttctcactctgtgaaaaatcagacaggtgatgtttctttggaaacctttatggtcagcgatgatgctgaattgaacactgag +# tcaccttctaatctcacaacagtgctagacacccgtactgtgagtgaacacacccagcctaaggggctaggagagattaccagctcatctttggacta +# ccgagatctacatgctacacaccgcatcgacaagttggccggtcagataagtgaattacaccctggccaccctctaccaagagccaaagtgattgcca +# actgctcggctgaaatattaagacataaggagctcattctactcttctcccgactcttgtcacgtttggacatgctgctgtcgtttgccaacgtggct +# atccaacatgacgacacaagacctatcgtggatcccagcagaaccaactttgagattcacttgaaggacaaagatttcaatgctgctgctgatccatt +# gttgatgtcaaactcatcatcagtattgtggactaagggagagagaaaaaccgtggcagagctttacgggctgtacgccagtatcatcttattggcgc +# actcaggtgcttatgttccagccaagtctgctaacattggtgttgtcagccacatgctttgtcaactccgctctacaaatcctttcctattacattca +# gcgcttgagaagtctatggatggccgtcgtatcatcatgtgcgacgctctgcctgacgatttcgaagactatggctcagccgatgacagattggaaag +# ctaccaagaaacactggcgactatgaatgtgggaactaaccgactgcttctaagatatcactga] +# protein sequence = [MLSHRHQKAALSKLQRSAYAHYPTPHSPISLTPNRSISSLPASKMLPAVNYVRQADPDAIVLLGHGESYHAFGRVDTN +# LIKGMVSAGNASEILYRDDGLTQWRFKSSRLPHNTSWLVKELNRPVVTLEWNEYATSFLQPLRNDSHWHFLSRTIPDGHSDHEFSVHSESQNIIVIGI +# QGKILKGIKTGEKSQRRVGITCFDPTNGSMSVQGTSLADLRSHIVSFRPRAIVLDSSLIGTYRDIIGTALLGSPEVKFTNMPPVLGSLQLLNDFFDVS +# GEIWRKCFDDYELYSLKAAVEFIKKTQKSTSAVFQLPSRELSSQWTVLLDKETISSLNLGSTGSHYMNSSWLLQRSIKTATVGGSRMLYKSILRPTRI +# VSEILFRQRAVQSIIKRKHTRQKLSSELSSLPDIPLLLQKLKIYPESEDLVSTILHSIDIISSLAPIASKFYMNGRQQDADFDDGDLIYRIPSAKRLE +# ELRDRARSLLNASDTMHGVVSSNTSIECNDRRTQKATLVNGVDCGPNRNTSFSHSVKNQTGDVSLETFMVSDDAELNTESPSNLTTVLDTRTVSEHTQ +# PKGLGEITSSSLDYRDLHATHRIDKLAGQISELHPGHPLPRAKVIANCSAEILRHKELILLFSRLLSRLDMLLSFANVAIQHDDTRPIVDPSRTNFEI +# HLKDKDFNAAADPLLMSNSSSVLWTKGERKTVAELYGLYASIILLAHSGAYVPAKSANIGVVSHMLCQLRSTNPFLLHSALEKSMDGRRIIMCDALPD +# DFEDYGSADDRLESYQETLATMNVGTNRLLLRYH] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g32 +### +# start gene g33 +pf_alpha_tbrc4498 AUGUSTUS gene 9028 10257 0.99 - . ID=g33 +pf_alpha_tbrc4498 AUGUSTUS transcript 9028 10257 0.99 - . ID=g33.t1;Parent=g33 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 9028 9030 . - 0 Parent=g33.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 9031 10257 0.99 - 0 ID=g33.t1.cds;Parent=g33.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 10255 10257 . - 0 Parent=g33.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# ttggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctattatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggcgcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaattga] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g33 +### +# start gene g34 +pf_alpha_tbrc4498 AUGUSTUS gene 10563 11084 1 + . ID=g34 +pf_alpha_tbrc4498 AUGUSTUS transcript 10563 11084 1 + . ID=g34.t1;Parent=g34 +pf_alpha_tbrc4498 AUGUSTUS start_codon 10563 10565 . + 0 Parent=g34.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 10563 11081 1 + 0 ID=g34.t1.cds;Parent=g34.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 11082 11084 . + 0 Parent=g34.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g34 +### +# start gene g35 +pf_alpha_tbrc4498 AUGUSTUS gene 11321 13090 0.99 - . ID=g35 +pf_alpha_tbrc4498 AUGUSTUS transcript 11321 13090 0.99 - . ID=g35.t1;Parent=g35 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 11321 11323 . - 0 Parent=g35.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 11324 13090 0.99 - 0 ID=g35.t1.cds;Parent=g35.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 13088 13090 . - 0 Parent=g35.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggacagcacgcggatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcaccaccaggagacttcatccaaggacgagcag +# ctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccgtttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtc +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaacgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgttcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgaaggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g35 +### +# start gene g36 +pf_alpha_tbrc4498 AUGUSTUS gene 13228 14464 0.46 + . ID=g36 +pf_alpha_tbrc4498 AUGUSTUS transcript 13228 14464 0.46 + . ID=g36.t1;Parent=g36 +pf_alpha_tbrc4498 AUGUSTUS start_codon 13228 13230 . + 0 Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS intron 13322 13685 0.46 + . Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 13228 13321 0.52 + 0 ID=g36.t1.cds;Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 13686 14461 0.71 + 2 ID=g36.t1.cds;Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 14462 14464 . + 0 Parent=g36.t1 +# coding sequence = [atgcagtcattagacacacaggcttccagtcgaggctacgcgagcattggtggtcctgacgagaaacagaaagggatag +# gctgtgctgtatgcttgtcacttgagagcatcaaggccggtcttctgctcgctcgtaacccaatcgtcaccccagatctcacacctctcgaaaatgca +# tacttcaactaccaggaggaactcgaacgcagactgatgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttctt +# caaggcacagcgtggacctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcagg +# aggttgtgattccaaaggatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacag +# ggtgatttgacaagtttggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaaactgccaagtttcgacgttac +# agagggtacaccattgcacagagtcgctgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcag +# tcttaaagtttgatggctgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgca +# ttcaagccacagaaggttgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctc +# tcgtgtctga] +# protein sequence = [MQSLDTQASSRGYASIGGPDEKQKGIGCAVCLSLESIKAGLLLARNPIVTPDLTPLENAYFNYQEELERRLMWTFPHY +# YYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNSRVTKADEQGDLTSLERQLPRTLYLLVK +# DGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFIIKSNILSGAFKPQKVEYAWLTREEIEEK +# VSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 1 +# RM: 1 +# incompatible hint groups: 2 +# RM: 2 +# end gene g36 +### +# start gene g37 +pf_alpha_tbrc4498 AUGUSTUS gene 14608 16005 1 - . ID=g37 +pf_alpha_tbrc4498 AUGUSTUS transcript 14608 16005 1 - . ID=g37.t1;Parent=g37 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 14608 14610 . - 0 Parent=g37.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 14611 16005 1 - 0 ID=g37.t1.cds;Parent=g37.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 16003 16005 . - 0 Parent=g37.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggtgacgctcgaggaagtggtagaggggatggaagaggtaacagaggtgatagaggtgacagaaacagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtaagtgatcgtgctgatgctagtcgtcacacatcgagaagagacgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatatttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccaccttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGDGRGNRGDRGDRNRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADASRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g37 +### +# start gene g38 +pf_alpha_tbrc4498 AUGUSTUS gene 16780 18078 0.9 + . ID=g38 +pf_alpha_tbrc4498 AUGUSTUS transcript 16780 18078 0.9 + . ID=g38.t1;Parent=g38 +pf_alpha_tbrc4498 AUGUSTUS start_codon 16780 16782 . + 0 Parent=g38.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 16780 18075 0.9 + 0 ID=g38.t1.cds;Parent=g38.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 18076 18078 . + 0 Parent=g38.t1 +# coding sequence = [atgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaact +# ccattcgtgctccaaggattcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagag +# gtcaaggaaatggatcaacaattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggc +# gctgattaaaggtgtgaaatcttctgctaagaaaagcacaggatcgacgagtcaaccattgggtgatattaggggcatgctgtctggaaagaaaaagt +# cagggagtaagtcacctcaccgccgtggcaccgaagacttgggtactgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttca +# gtgggggacgctctaggctcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaagaattgcgttccgccaaagagcttctcaagga +# tgatgaaagtcataaagctatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatc +# ttgagatgatgaagcgcaggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaat +# gagtttgaagaggttgacgagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacattaagcct +# cggtgagattgagcaaaaggccaaccagcacatggactgcattccaaaaattctcaacggtcgtcttgactccacttacctacaaaaagcgaaagtat +# tcatgcacgactctggagcaacaatcttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaa +# ggggctattgccaatctgatcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacatt +# cacattacacattctcgctccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacag +# ttgattatggtcaatacatcgctgatttggtcccttgtgaatga] +# protein sequence = [MSSGEEEQEGSRHRIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRET +# KKGEDVAPIPRKRALIKGVKSSAKKSTGSTSQPLGDIRGMLSGKKKSGSKSPHRRGTEDLGTEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGP +# SSQELRSAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVK +# RKYGNEEFPGTLSLGEIEQKANQHMDCIPKILNGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKA +# EHNRVIQFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 6 +# RM: 6 +# end gene g38 +### +# start gene g39 +pf_alpha_tbrc4498 AUGUSTUS gene 18536 21661 1 + . ID=g39 +pf_alpha_tbrc4498 AUGUSTUS transcript 18536 21661 1 + . ID=g39.t1;Parent=g39 +pf_alpha_tbrc4498 AUGUSTUS start_codon 18536 18538 . + 0 Parent=g39.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 18536 21658 1 + 0 ID=g39.t1.cds;Parent=g39.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 21659 21661 . + 0 Parent=g39.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgaaaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g39 +### +# command line: +# augustus --species=pichia_stipitis --genemodel=complete --strand=both --protein=on --codingseq=on --gff3=on windows.fa diff --git a/results/2026-10-30_petasospora/data/aug_saccharomyces_cerevisiae_S288C.gff3 b/results/2026-10-30_petasospora/data/aug_saccharomyces_cerevisiae_S288C.gff3 new file mode 100644 index 00000000..8616eafa --- /dev/null +++ b/results/2026-10-30_petasospora/data/aug_saccharomyces_cerevisiae_S288C.gff3 @@ -0,0 +1,1666 @@ +##gff-version 3 +# This output was generated with AUGUSTUS (version 3.5.0). +# AUGUSTUS is a gene prediction tool written by M. Stanke (mario.stanke@uni-greifswald.de), +# O. Keller, S. König, L. Gerischer, L. Romoth, Katharina Hoff, Henry Mehlan and Daniel Honsel. +# Please cite: Mario Stanke, Mark Diekhans, Robert Baertsch, David Haussler (2008), +# Using native and syntenically mapped cDNA alignments to improve de novo gene finding +# Bioinformatics 24: 637-644, doi 10.1093/bioinformatics/btn013 +# No extrinsic information on sequences given. +# Sources of extrinsic information: M RM +# Initializing the parameters using config directory /opt/linux/centos/8.x/x86_64/pkgs/augustus/3.5.0/env/config/ ... +# saccharomyces_cerevisiae_S288C version. Using default transition matrix. +# Looks like windows.fa is in fasta format. +# We have hints for 0 sequences and for 0 of the sequences in the input set. +# +# ----- prediction on sequence number 1 (length = 19001, name = pf_a_cp048715) ----- +# +# Predicted genes for sequence number 1 on both strands +# start gene g1 +pf_a_cp048715 AUGUSTUS gene 746 1603 0.54 - . ID=g1 +pf_a_cp048715 AUGUSTUS transcript 746 1603 0.54 - . ID=g1.t1;Parent=g1 +pf_a_cp048715 AUGUSTUS stop_codon 746 748 . - 0 Parent=g1.t1 +pf_a_cp048715 AUGUSTUS CDS 749 1603 0.54 - 0 ID=g1.t1.cds;Parent=g1.t1 +pf_a_cp048715 AUGUSTUS start_codon 1601 1603 . - 0 Parent=g1.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagaccagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcggaatacattagggcacaggatttcaagtatatcgagacgatttcagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# agaagagattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgatgaagatgac +# accagtgaagacacagagcgactcgtttctgacgctgctgatattatctggccaaagtcctatgattctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgacctcgctcgactattcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEDQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETISDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVKKRFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSDAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g1 +### +# start gene g2 +pf_a_cp048715 AUGUSTUS gene 1950 2876 0.56 - . ID=g2 +pf_a_cp048715 AUGUSTUS transcript 1950 2876 0.56 - . ID=g2.t1;Parent=g2 +pf_a_cp048715 AUGUSTUS stop_codon 1950 1952 . - 0 Parent=g2.t1 +pf_a_cp048715 AUGUSTUS CDS 1953 2876 0.56 - 0 ID=g2.t1.cds;Parent=g2.t1 +pf_a_cp048715 AUGUSTUS start_codon 2874 2876 . - 0 Parent=g2.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# cagcacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccagatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttgtctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggagtgctcatgacctcgtcacaggttgtcacctatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgagcaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaatactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVTARQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILSTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g2 +### +# start gene g3 +pf_a_cp048715 AUGUSTUS gene 3351 3743 0.27 - . ID=g3 +pf_a_cp048715 AUGUSTUS transcript 3351 3743 0.27 - . ID=g3.t1;Parent=g3 +pf_a_cp048715 AUGUSTUS stop_codon 3351 3353 . - 0 Parent=g3.t1 +pf_a_cp048715 AUGUSTUS CDS 3354 3743 0.27 - 0 ID=g3.t1.cds;Parent=g3.t1 +pf_a_cp048715 AUGUSTUS start_codon 3741 3743 . - 0 Parent=g3.t1 +# coding sequence = [atgtcagttatctttttgaacgatccggaaaactcaatcaagcagagtaagcaaaagaccgaaaaagcttcagctagtg +# atatcaaagatatgagcagttatgtcaagctttgcactagtgtttatgaggaagacgactgcaaacggaggggggtcgatgctctccacgattcagat +# gactacaaagaccatgtgcttgtattaacgcttaacgagcctaatatgaaagctccggttgactctgctagtacaaacgccaggacaacatataaggt +# aacaaagcccacaaaaaagaagaagacgccgcctcatttggagcttgtattcagtaaattcaggtcccctttgtatggtgaagccgaggatatttgca +# tcaacagttcccctatgtga] +# protein sequence = [MSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAP +# VDSASTNARTTYKVTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g3 +### +# start gene g4 +pf_a_cp048715 AUGUSTUS gene 3933 4364 0.22 - . ID=g4 +pf_a_cp048715 AUGUSTUS transcript 3933 4364 0.22 - . ID=g4.t1;Parent=g4 +pf_a_cp048715 AUGUSTUS stop_codon 3933 3935 . - 0 Parent=g4.t1 +pf_a_cp048715 AUGUSTUS CDS 3936 4364 0.22 - 0 ID=g4.t1.cds;Parent=g4.t1 +pf_a_cp048715 AUGUSTUS start_codon 4362 4364 . - 0 Parent=g4.t1 +# coding sequence = [atggttactattggcgcccagtgctgcagaaacggagaagcacctccgtcgataagcacctttcccatcctctcgaaaa +# tatcattcaccctcaccaagacagacctaccaactaaagtcttttgggaaaagaagcagtttccagtggcgagccgactagctgctggagcaaacgct +# ccccgcagtcccaaagagattacttttgcccatctatcttccggaaccagcacaaaatcgatctatgtttacgctccacacaacaacgttgcacgtcc +# tcgtaataagttcatcattgccaggactatactgagccggattgtcaagaccagacacccttggtcgactaacgatgtctccaaaatcatatccaaag +# taggtacattctctatgaacttcagtcacacgcacagcatctcaacggtcgaatactaa] +# protein sequence = [MVTIGAQCCRNGEAPPSISTFPILSKISFTLTKTDLPTKVFWEKKQFPVASRLAAGANAPRSPKEITFAHLSSGTSTK +# SIYVYAPHNNVARPRNKFIIARTILSRIVKTRHPWSTNDVSKIISKVGTFSMNFSHTHSISTVEY] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 0 +# end gene g4 +### +# start gene g5 +pf_a_cp048715 AUGUSTUS gene 4938 5637 0.33 + . ID=g5 +pf_a_cp048715 AUGUSTUS transcript 4938 5637 0.33 + . ID=g5.t1;Parent=g5 +pf_a_cp048715 AUGUSTUS start_codon 4938 4940 . + 0 Parent=g5.t1 +pf_a_cp048715 AUGUSTUS intron 5239 5335 0.4 + . Parent=g5.t1 +pf_a_cp048715 AUGUSTUS CDS 4938 5238 0.83 + 0 ID=g5.t1.cds;Parent=g5.t1 +pf_a_cp048715 AUGUSTUS CDS 5336 5634 0.4 + 2 ID=g5.t1.cds;Parent=g5.t1 +pf_a_cp048715 AUGUSTUS stop_codon 5635 5637 . + 0 Parent=g5.t1 +# coding sequence = [atggccacgagggatcaaatagcgtataaggaggcaagcgagaagcttgatgcccttatcgagttttcatctaacttct +# cgactaacccaacgaaaccgagatatgacaaaataatgctggaagatccgtttgagaaggacagtttactgagcaaggttccggcctatgcgagagca +# gcattggaaactctgatctcaaacttcgaactatccctcggacgtgttatggtaggacaatctctttcccataaatctaaccaatcggtcataaaaac +# taacaaaccattaggaccacgactcaaagaccccgaggcgtacggacacagaatagcatcgtacgaagtccagtctaccactatcgaagtaacgggat +# cgacttctagccctgtctttccaggtggaggtcgcaagcgaaatgcgcttccagcaaagaagcgaacatcaattacccctgaatccaaggagtttttg +# gagagagtgtttaaggctaagaggactccaaacagtacggagaggaaagttatagcagaaaagtgtggactcacgccactgcaggttagggtatggtt +# cacaaacaagagaatgaggaacaaggaaggctga] +# protein sequence = [MATRDQIAYKEASEKLDALIEFSSNFSTNPTKPRYDKIMLEDPFEKDSLLSKVPAYARAALETLISNFELSLGRVMVG +# QSLSHKSNQSVIKTNKPLGPRLKDPEAYGHRIASYEVQSTTIEVTGSTSSPVFPGGGRKRNALPAKKRTSITPESKEFLERVFKAKRTPNSTERKVIA +# EKCGLTPLQVRVWFTNKRMRNKEG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 2 +# RM: 2 +# incompatible hint groups: 0 +# end gene g5 +### +# start gene g6 +pf_a_cp048715 AUGUSTUS gene 5742 6971 1 - . ID=g6 +pf_a_cp048715 AUGUSTUS transcript 5742 6971 1 - . ID=g6.t1;Parent=g6 +pf_a_cp048715 AUGUSTUS stop_codon 5742 5744 . - 0 Parent=g6.t1 +pf_a_cp048715 AUGUSTUS CDS 5745 6971 1 - 0 ID=g6.t1.cds;Parent=g6.t1 +pf_a_cp048715 AUGUSTUS start_codon 6969 6971 . - 0 Parent=g6.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacgagacaagagctcgagaagacggtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcacaggaatcaccgacgactcccagacatccaaacta +# cagctcatctactatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggctcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaactag] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLTGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g6 +### +# start gene g7 +pf_a_cp048715 AUGUSTUS gene 7276 7797 1 + . ID=g7 +pf_a_cp048715 AUGUSTUS transcript 7276 7797 1 + . ID=g7.t1;Parent=g7 +pf_a_cp048715 AUGUSTUS start_codon 7276 7278 . + 0 Parent=g7.t1 +pf_a_cp048715 AUGUSTUS CDS 7276 7794 1 + 0 ID=g7.t1.cds;Parent=g7.t1 +pf_a_cp048715 AUGUSTUS stop_codon 7795 7797 . + 0 Parent=g7.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g7 +### +# start gene g8 +pf_a_cp048715 AUGUSTUS gene 8035 9804 0.81 - . ID=g8 +pf_a_cp048715 AUGUSTUS transcript 8035 9804 0.81 - . ID=g8.t1;Parent=g8 +pf_a_cp048715 AUGUSTUS stop_codon 8035 8037 . - 0 Parent=g8.t1 +pf_a_cp048715 AUGUSTUS CDS 8038 9804 0.81 - 0 ID=g8.t1.cds;Parent=g8.t1 +pf_a_cp048715 AUGUSTUS start_codon 9802 9804 . - 0 Parent=g8.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccgggacagcacgctgatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtcgtttcggcacatcctgaacatcaccaccaggagacttcatccaaggacgagcag +# ctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccatttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaatcttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatcagtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtt +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gatactgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgaggtccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaacgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgtgcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgagggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g8 +### +# start gene g9 +pf_a_cp048715 AUGUSTUS gene 10516 11175 0.88 + . ID=g9 +pf_a_cp048715 AUGUSTUS transcript 10516 11175 0.88 + . ID=g9.t1;Parent=g9 +pf_a_cp048715 AUGUSTUS start_codon 10516 10518 . + 0 Parent=g9.t1 +pf_a_cp048715 AUGUSTUS CDS 10516 11172 0.88 + 0 ID=g9.t1.cds;Parent=g9.t1 +pf_a_cp048715 AUGUSTUS stop_codon 11173 11175 . + 0 Parent=g9.t1 +# coding sequence = [atgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggac +# ctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaag +# gatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagttt +# ggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcgacgttacagagggtacaccattgc +# acagagtcgccgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttaaagtttgatggc +# tgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggt +# tgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNS +# RVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFII +# KSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g9 +### +# start gene g10 +pf_a_cp048715 AUGUSTUS gene 11319 12716 1 - . ID=g10 +pf_a_cp048715 AUGUSTUS transcript 11319 12716 1 - . ID=g10.t1;Parent=g10 +pf_a_cp048715 AUGUSTUS stop_codon 11319 11321 . - 0 Parent=g10.t1 +pf_a_cp048715 AUGUSTUS CDS 11322 12716 1 - 0 ID=g10.t1.cds;Parent=g10.t1 +pf_a_cp048715 AUGUSTUS start_codon 12714 12716 . - 0 Parent=g10.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggtgacgctcgaggaagtggtagaggagaaggaagaggtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaatgtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatttttgtcggtcttgtga +# ccggtaagatctctgttgttatcaagggcggtgatggtgcctttgccaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggcatcatctcactctggtccaccacagacctatcattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccatcttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRGNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHECMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFANACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLSLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g10 +### +# start gene g11 +pf_a_cp048715 AUGUSTUS gene 12921 14789 1 + . ID=g11 +pf_a_cp048715 AUGUSTUS transcript 12921 14789 1 + . ID=g11.t1;Parent=g11 +pf_a_cp048715 AUGUSTUS start_codon 12921 12923 . + 0 Parent=g11.t1 +pf_a_cp048715 AUGUSTUS CDS 12921 14786 1 + 0 ID=g11.t1.cds;Parent=g11.t1 +pf_a_cp048715 AUGUSTUS stop_codon 14787 14789 . + 0 Parent=g11.t1 +# coding sequence = [atgaattcccaacggcatacaccctatattcgagctgactcctccagtgtgctaccgaggacgctgggccgtcgaccgc +# ccggccctgtcatcccgaacctgggagctaagcaccatcacagctctcttaacaaaccccctcgatcatctcgacagcatgatgaacgagagtcttca +# tcacctcccagaccacacagctcctttgccccacctatcaactccaattcctcaaccacgtccgcctctagtcccaaaaaaaacgttaccgatgggcg +# ggaccctgccaacttgatctccacaacgtacgcctactgcggcaacaggaaacgactgcagaatccaagggatgtcgaaaataaaaagagactaagag +# agatggaagcaaaaaatgctcctggggaaccgccggtggctctacagtctaaaaggtttcaacaggaagcgtcagttgtcaaggcgaatgatggggac +# acctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctttggagaaacgtgccccaat +# aatgtctccaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcgatcagacgctgacttcatgaactccattcgtgctccaagga +# tccccagtcgagctgctcaagctgactacgagaagcacactgctagcgctcgtctacaacataaacgtgaggatacagaggtcaagggaatggatcaa +# caattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcgctgattaaaggtgtgaa +# atcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtcagggagtaagtcacctc +# accgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccggggccgtcatgaaggttcagtgggggacgctctaggc +# tcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaaggatgatgaaagtcataaagc +# tatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatcttgagatgatgaagcgca +# ggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatgagtttgaagaggttgac +# gagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagtttccgggaacattaaccctcggtgagattgagcaaaa +# ggtcaaccagcacatggactgcattccaaaaattctcaacggtcgtcttgactccacttacctgcaaaaagcgaaagtattcatgcacgactctggag +# caacaatcttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaaaagacaaggggctattgctaatcta +# atcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattcacattacacattctcgc +# tccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagttgattatggtcaataca +# tcgctgatttggtcccttgtgaatga] +# protein sequence = [MNSQRHTPYIRADSSSVLPRTLGRRPPGPVIPNLGAKHHHSSLNKPPRSSRQHDERESSSPPRPHSSFAPPINSNSST +# TSASSPKKNVTDGRDPANLISTTYAYCGNRKRLQNPRDVENKKRLREMEAKNAPGEPPVALQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNR +# KRNILSSLEKRAPIMSPGEEEQEGSRHRIPSIRSDADFMNSIRAPRIPSRAAQADYEKHTASARLQHKREDTEVKGMDQQLHDDTNSGKRETKKGEDV +# APIPRKRALIKGVKSSAKKSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRGRHEGSVGDALGSDEELFNFDSPGPSSQELR +# SAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNE +# EFPGTLTLGEIEQKVNQHMDCIPKILNGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVI +# QFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g11 +### +# start gene g12 +pf_a_cp048715 AUGUSTUS gene 15247 18372 1 + . ID=g12 +pf_a_cp048715 AUGUSTUS transcript 15247 18372 1 + . ID=g12.t1;Parent=g12 +pf_a_cp048715 AUGUSTUS start_codon 15247 15249 . + 0 Parent=g12.t1 +pf_a_cp048715 AUGUSTUS CDS 15247 18369 1 + 0 ID=g12.t1.cds;Parent=g12.t1 +pf_a_cp048715 AUGUSTUS stop_codon 18370 18372 . + 0 Parent=g12.t1 +# coding sequence = [atgtcgaagacaatctccgactccgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctatggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgccgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctctaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagttagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaaaaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaaaaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacctgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacatcaatggtgatcttggtgatgcagttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctattgccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgaccaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcttccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagtgaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacgtctgcctgcagatctttggtcaacca +# agttcaggatattttgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagatcagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAIAVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 18 +# RM: 18 +# end gene g12 +### +# +# ----- prediction on sequence number 2 (length = 19501, name = pf_a_jcm3601) ----- +# +# Predicted genes for sequence number 2 on both strands +# start gene g13 +pf_a_jcm3601 AUGUSTUS gene 944 1801 0.92 - . ID=g13 +pf_a_jcm3601 AUGUSTUS transcript 944 1801 0.92 - . ID=g13.t1;Parent=g13 +pf_a_jcm3601 AUGUSTUS stop_codon 944 946 . - 0 Parent=g13.t1 +pf_a_jcm3601 AUGUSTUS CDS 947 1801 0.92 - 0 ID=g13.t1.cds;Parent=g13.t1 +pf_a_jcm3601 AUGUSTUS start_codon 1799 1801 . - 0 Parent=g13.t1 +# coding sequence = [atgattagtactaacagtcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctgaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgacgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgaatctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNSHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYESINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g13 +### +# start gene g14 +pf_a_jcm3601 AUGUSTUS gene 2148 3074 0.93 - . ID=g14 +pf_a_jcm3601 AUGUSTUS transcript 2148 3074 0.93 - . ID=g14.t1;Parent=g14 +pf_a_jcm3601 AUGUSTUS stop_codon 2148 2150 . - 0 Parent=g14.t1 +pf_a_jcm3601 AUGUSTUS CDS 2151 3074 0.93 - 0 ID=g14.t1.cds;Parent=g14.t1 +pf_a_jcm3601 AUGUSTUS start_codon 3072 3074 . - 0 Parent=g14.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtgg +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttttctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggttgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctggt +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVAPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g14 +### +# start gene g15 +pf_a_jcm3601 AUGUSTUS gene 3549 4562 0.73 - . ID=g15 +pf_a_jcm3601 AUGUSTUS transcript 3549 4562 0.73 - . ID=g15.t1;Parent=g15 +pf_a_jcm3601 AUGUSTUS stop_codon 3549 3551 . - 0 Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS intron 4119 4190 0.74 - . Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS CDS 3552 4118 0.73 - 0 ID=g15.t1.cds;Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS CDS 4191 4562 0.75 - 0 ID=g15.t1.cds;Parent=g15.t1 +pf_a_jcm3601 AUGUSTUS start_codon 4560 4562 . - 0 Parent=g15.t1 +# coding sequence = [atggttactattggcgcccagtgctgcagaaacggagaagcacctccgtcgataagcacctttcccatcctctcgaaaa +# tatcattcaccctcaccaagacagatctaccaactaaagtcttttgggaaaagaagcagtttccagtggcgagcccactagctgctggagcaaacgct +# ccccgcagtcccaaagagattacttttgcccatctatcttccggaaccagcacaaaatcgatctatgtttatgctccacacaacaacgttgcacgtcc +# tcgtaataagttcatcattgccaggactatactgagccggattgtcaagaccagacacccttggtcgactaacgatgtctccaaaatcatatccaaaa +# tctggggctcaggaaatcgcttattcttgagctattttgactatctctcctttcttgaaaacgagtcacacgaatgtcgctatccactctaccattac +# aatccgcggtcccgaagcacagttgctaaagccagtgcttgtactgctagtgctaagaacagtgagttagaacagaacatgtcagttatctttttgaa +# cgatccggaaaactcaatcaagcagagtaagcaaaagaccgaaaaagcttcagctagtgatatcaaagatatgagcagttatgtcaagctttgcacta +# gtgtttatgaggaagacgactgcaaacggaggggggtcgatgctctccacgattcagatgactacaaagaccatgtgcttgtattaacgcttaacgag +# cctaatatgaaagctccggttggctctgctagtacaaacgccaggacaacatataaggtaacaaagcccacaaaaaagaagaagacgccgcctcattt +# ggagcttgtattcagtaaattcaggtcccctttgtatggtgaagccgaggatatttgcatcaacagttcccctatgtga] +# protein sequence = [MVTIGAQCCRNGEAPPSISTFPILSKISFTLTKTDLPTKVFWEKKQFPVASPLAAGANAPRSPKEITFAHLSSGTSTK +# SIYVYAPHNNVARPRNKFIIARTILSRIVKTRHPWSTNDVSKIISKIWGSGNRLFLSYFDYLSFLENESHECRYPLYHYNPRSRSTVAKASACTASAK +# NSELEQNMSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAPVGSASTNARTTYK +# VTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g15 +### +# start gene g16 +pf_a_jcm3601 AUGUSTUS gene 5136 5835 0.28 + . ID=g16 +pf_a_jcm3601 AUGUSTUS transcript 5136 5835 0.28 + . ID=g16.t1;Parent=g16 +pf_a_jcm3601 AUGUSTUS start_codon 5136 5138 . + 0 Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS intron 5437 5533 0.32 + . Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS CDS 5136 5436 0.61 + 0 ID=g16.t1.cds;Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS CDS 5534 5832 0.32 + 2 ID=g16.t1.cds;Parent=g16.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 5833 5835 . + 0 Parent=g16.t1 +# coding sequence = [atggccacgagggatcaaatagcgtataaggaggcaagcgagaagcttgatgcccttatcgagttttcatctaacttct +# cgactaacccaacgaaaccgagatatgacaaaataatgctggaagatccgtttgagaaggagagtttactgagcaaggttccggcctatgcgagagca +# gcattggaaactctgatctcaaacttcgaactatccctcggacgtgttatggtaggacaatctctttcccataaatctaaccaatcggtcataaaaac +# taacaaaccattaggaccacgactcaaagaccccgaggcgtacggacacagaatagcatcgtacgaagtccagtctaccactatcgaagtaacgggat +# cgacttctagccctgtctttccaggtggaggtcgcaagcgaaatgcgcttccagcaaagaagcgaacatcaattacccctgaatccaaggagtttttg +# gagagagtgtttaaggctaagaggactccaaacagtacggagaggaaagttatagcagaaaagtgtggactcacgccactgcaggttagggtatggtt +# cacaaacaagagaatgaggaacaaggaaggctga] +# protein sequence = [MATRDQIAYKEASEKLDALIEFSSNFSTNPTKPRYDKIMLEDPFEKESLLSKVPAYARAALETLISNFELSLGRVMVG +# QSLSHKSNQSVIKTNKPLGPRLKDPEAYGHRIASYEVQSTTIEVTGSTSSPVFPGGGRKRNALPAKKRTSITPESKEFLERVFKAKRTPNSTERKVIA +# EKCGLTPLQVRVWFTNKRMRNKEG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/2 +# CDS introns: 0/1 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 1 +# RM: 1 +# incompatible hint groups: 1 +# RM: 1 +# end gene g16 +### +# start gene g17 +pf_a_jcm3601 AUGUSTUS gene 5937 7166 0.94 - . ID=g17 +pf_a_jcm3601 AUGUSTUS transcript 5937 7166 0.94 - . ID=g17.t1;Parent=g17 +pf_a_jcm3601 AUGUSTUS stop_codon 5937 5939 . - 0 Parent=g17.t1 +pf_a_jcm3601 AUGUSTUS CDS 5940 7166 0.94 - 0 ID=g17.t1.cds;Parent=g17.t1 +pf_a_jcm3601 AUGUSTUS start_codon 7164 7166 . - 0 Parent=g17.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctactatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggctcttggtgctattgtcaaaagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaactag] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g17 +### +# start gene g18 +pf_a_jcm3601 AUGUSTUS gene 7472 7993 0.99 + . ID=g18 +pf_a_jcm3601 AUGUSTUS transcript 7472 7993 0.99 + . ID=g18.t1;Parent=g18 +pf_a_jcm3601 AUGUSTUS start_codon 7472 7474 . + 0 Parent=g18.t1 +pf_a_jcm3601 AUGUSTUS CDS 7472 7990 0.99 + 0 ID=g18.t1.cds;Parent=g18.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 7991 7993 . + 0 Parent=g18.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgaaatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g18 +### +# start gene g19 +pf_a_jcm3601 AUGUSTUS gene 8231 10000 0.79 - . ID=g19 +pf_a_jcm3601 AUGUSTUS transcript 8231 10000 0.79 - . ID=g19.t1;Parent=g19 +pf_a_jcm3601 AUGUSTUS stop_codon 8231 8233 . - 0 Parent=g19.t1 +pf_a_jcm3601 AUGUSTUS CDS 8234 10000 0.79 - 0 ID=g19.t1.cds;Parent=g19.t1 +pf_a_jcm3601 AUGUSTUS start_codon 9998 10000 . - 0 Parent=g19.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggacagcacgctgatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcacgaccaggagacttcatccaaggacgagcag +# ctggagaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccatttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaggcatttacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtc +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcaaacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaatgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgtgcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgagggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHDQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g19 +### +# start gene g20 +pf_a_jcm3601 AUGUSTUS gene 10711 11370 0.92 + . ID=g20 +pf_a_jcm3601 AUGUSTUS transcript 10711 11370 0.92 + . ID=g20.t1;Parent=g20 +pf_a_jcm3601 AUGUSTUS start_codon 10711 10713 . + 0 Parent=g20.t1 +pf_a_jcm3601 AUGUSTUS CDS 10711 11367 0.92 + 0 ID=g20.t1.cds;Parent=g20.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 11368 11370 . + 0 Parent=g20.t1 +# coding sequence = [atgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggac +# ctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaag +# gatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagttt +# ggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcgacgttacagagggtacaccattgc +# acagagtcgccgaggagggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttgaagtttgatggc +# tgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggt +# tgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNS +# RVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEEGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFII +# KSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g20 +### +# start gene g21 +pf_a_jcm3601 AUGUSTUS gene 11514 12911 1 - . ID=g21 +pf_a_jcm3601 AUGUSTUS transcript 11514 12911 1 - . ID=g21.t1;Parent=g21 +pf_a_jcm3601 AUGUSTUS stop_codon 11514 11516 . - 0 Parent=g21.t1 +pf_a_jcm3601 AUGUSTUS CDS 11517 12911 1 - 0 ID=g21.t1.cds;Parent=g21.t1 +pf_a_jcm3601 AUGUSTUS start_codon 12909 12911 . - 0 Parent=g21.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggcgacgctcgaggaagtggtagaggggaaggaagaagtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatatttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccaccttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRSNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g21 +### +# start gene g22 +pf_a_jcm3601 AUGUSTUS gene 13116 14984 0.98 + . ID=g22 +pf_a_jcm3601 AUGUSTUS transcript 13116 14984 0.98 + . ID=g22.t1;Parent=g22 +pf_a_jcm3601 AUGUSTUS start_codon 13116 13118 . + 0 Parent=g22.t1 +pf_a_jcm3601 AUGUSTUS CDS 13116 14981 0.98 + 0 ID=g22.t1.cds;Parent=g22.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 14982 14984 . + 0 Parent=g22.t1 +# coding sequence = [atgaattcccaacggcatacaccctatattcgagctgactcctcccgtgtgctaccgaggacgctgggccgtcgaccgc +# ccggccctgtcatcccgaacctgggagctaagcaccatcacagctctctcaacaaaccccctcgatcatctcgacagcatgatgaacgagagtcttca +# tcacctcccagaccacacagctcctttgccccacctatcaactccaattcctcaatcacgtccgcctctagtcccaaaaaaaacgttaccgatgggcg +# ggaccccgccaacttgatctccacaacgtacgcctactgcggcaacaggaaacgactgcagaatccaagggaagtcgaaaataaaaagagactaagag +# agatggaagcaaaaaatgctcctggggaaccgccggtgcctctacagtctaaaaggtttcaacaggaagcgtcagttgtcaaggcgaatgatggggac +# acctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctagggagaaacgtgccccaat +# aatgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaactccattcgtgctccaagga +# ttcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagaggtcaaggaaatggatcaa +# caattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcgctgattaaaggtgtgaa +# atcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtcagggagtaagtcacctc +# accgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttcagtgggggacgctctaggc +# tcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaaggatgatgaaagtcataaagc +# tatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatcttgagatgatgaaacgca +# ggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatgagtttgaagaggttgac +# gagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacattaagcctcggtgagattgagcaaaa +# ggccaaccagcacatggactgcattccaaaaattctcgacggtcgtcttgactccacttacctacaaaaagcgaaagtattcatgcacgactctggag +# caacaattttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaaggggctattgccaatctg +# atcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattcacattacacattctcgc +# tccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagttgattatggtcaataca +# tcgctgatttggtcccttgtgaatga] +# protein sequence = [MNSQRHTPYIRADSSRVLPRTLGRRPPGPVIPNLGAKHHHSSLNKPPRSSRQHDERESSSPPRPHSSFAPPINSNSSI +# TSASSPKKNVTDGRDPANLISTTYAYCGNRKRLQNPREVENKKRLREMEAKNAPGEPPVPLQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNR +# KRNILSSREKRAPIMSSGEEEQEGSRHRIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRETKKGEDV +# APIPRKRALIKGVKSSAKKSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGPSSQELR +# SAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNE +# EFPGTLSLGEIEQKANQHMDCIPKILDGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVI +# QFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g22 +### +# start gene g23 +pf_a_jcm3601 AUGUSTUS gene 15442 18567 1 + . ID=g23 +pf_a_jcm3601 AUGUSTUS transcript 15442 18567 1 + . ID=g23.t1;Parent=g23 +pf_a_jcm3601 AUGUSTUS start_codon 15442 15444 . + 0 Parent=g23.t1 +pf_a_jcm3601 AUGUSTUS CDS 15442 18564 1 + 0 ID=g23.t1.cds;Parent=g23.t1 +pf_a_jcm3601 AUGUSTUS stop_codon 18565 18567 . + 0 Parent=g23.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatatggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagcagctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatgttgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDVDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g23 +### +# +# ----- prediction on sequence number 3 (length = 22001, name = pf_alpha_strain65) ----- +# +# Predicted genes for sequence number 3 on both strands +# start gene g24 +pf_alpha_strain65 AUGUSTUS gene 704 1561 0.64 - . ID=g24 +pf_alpha_strain65 AUGUSTUS transcript 704 1561 0.64 - . ID=g24.t1;Parent=g24 +pf_alpha_strain65 AUGUSTUS stop_codon 704 706 . - 0 Parent=g24.t1 +pf_alpha_strain65 AUGUSTUS CDS 707 1561 0.64 - 0 ID=g24.t1.cds;Parent=g24.t1 +pf_alpha_strain65 AUGUSTUS start_codon 1559 1561 . - 0 Parent=g24.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaactgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccgctgcaagcaagaagaagtcgagtgcaaacttgagtgatgatgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgactctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAAAASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g24 +### +# start gene g25 +pf_alpha_strain65 AUGUSTUS gene 1908 2834 0.65 - . ID=g25 +pf_alpha_strain65 AUGUSTUS transcript 1908 2834 0.65 - . ID=g25.t1;Parent=g25 +pf_alpha_strain65 AUGUSTUS stop_codon 1908 1910 . - 0 Parent=g25.t1 +pf_alpha_strain65 AUGUSTUS CDS 1911 2834 0.65 - 0 ID=g25.t1.cds;Parent=g25.t1 +pf_alpha_strain65 AUGUSTUS start_codon 2832 2834 . - 0 Parent=g25.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccctcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttttctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggtctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTLSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g25 +### +# start gene g26 +pf_alpha_strain65 AUGUSTUS gene 3495 5891 0.99 + . ID=g26 +pf_alpha_strain65 AUGUSTUS transcript 3495 5891 0.99 + . ID=g26.t1;Parent=g26 +pf_alpha_strain65 AUGUSTUS start_codon 3495 3497 . + 0 Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS CDS 3495 5888 0.99 + 0 ID=g26.t1.cds;Parent=g26.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 5889 5891 . + 0 Parent=g26.t1 +# coding sequence = [atgttgtcccaccgccatcagaaagctgctctctccaaactgcaaagaagtgcttacgcacattacccaacaccacatt +# cgccaatatctctaacaccaaatagatcaatatcgtctcttccggcctcgaagatgcttccagctgtcaattacgttcgccaagcagatccagatgcg +# attgttcttctcggtcatggtgaatcttaccatgctttcggcagagtcgatactaatctgattaaaggaatggtatcagctggcaatgcaagcgagat +# actctaccgtgatgatggactgacacaatggagattcaagtcatctcgactcccgcataacacttcttggctggtgaaggagttgaacaggccagttg +# tcaccttggaatggaacgaatatgctactagtttcctacagccattgcgcaacgactcccattggcatttcctatcgagaaccattcctgatggccac +# tcagaccatgaattctctgttcattctgaatctcaaaacatcatagtaattggtatacaggggaagattctcaaggggatcaaaacaggtgaaaagtc +# acagagacgtgttggtatcacttgctttgacccaacaaacggatcgatgtctgttcagggcacttctcttgctgatttgagatcccatatcgtgagct +# ttcgtcctcgggctattgttctggactccagtctgattgggacataccgggacatcattggcacagctcttctaggttcaccagaagttaagtttacc +# aatatgcctcccgtgttgggctcgctacaactccttaatgatttctttgatgtctctggtgaaatttggaggaagtgtttcgatgattacgagctgta +# ctcactgaaggctgctgtggagttcattaagaagacacagaaatcaacgtctgcagtattccaactaccatcgagggaactgtcgtcacaatggaccg +# tgttattggacaaagaaactatcagttccttgaacctgggctccaccgggagtcactatatgaattcctcctggttacttcaaagatcaatcaagact +# gctactgttggtggttctcgaatgttgtacaagtctatcttgaggccaactaggatagtcagtgagattttgtttcgtcagagggcggttcaatccat +# cattaaaaggaaacatacacgtcagaagctgagctcagagctctccagtttaccagacatcccattgctactacagaaactcaagatctatccggaat +# cagaagaccttgtcagtactatactacactccatcgacatcatctcgtcacttgcccccatcgcctccaagttttacatgaacggtcaccagcaagac +# gctgactttgatgatggtgacttgatttaccgcattccatctgcaaaacgtttggaggaactcagagacagggcccgttcccttctcaatgctagcga +# cacaatgcatggtgttgtttcctcgaatacaagcattgaatgcaatgatcggaggacacagaaagctactttggtgaatggtgttgattgtggaccta +# ataggaacacttctttttctcactctgtgaaaaatcagacaggtgatgtttctttggaaacctttatggtcagcgatgatgctgaattgaacactgag +# tcaccttctaatctcacaacagtgctagacacccgtactgtgagtgaacacacccagcctaaggggctaggagagattaccagctcatctttggacta +# ccgagatctacatgctacacaccgcatcgacaagttggccggtcagataagtgaattacaccctggccaccctctaccaagagccaaagtgattgcca +# actgctcggctgaaatattaagacataaggagctcattctactcttctcccgactcttgtcacgtttggacatgctgctgtcgtttgccaacgtggct +# atccaacatgacgacacaagacctatcgtggatcccagcagaaccaactttgagattcacttgaaggacaaagatttcaatgctgctgctgatccatt +# gttgatgtcaaactcatcatcagtattgtggactaagggagagagaaaaaccgtggcagagctttacgggctgtacgccagtatcatcttattggcgc +# actcaggtgcttatgttccagccaagtctgctaacattggtgttgtcagccacatgctttgtcaactccgctctacaaatcctttcctattacattca +# gcgcttgagaagtctatggatggccgtcgtatcatcatgtgcgacgctctgcctgacgatttcgaagactatggctcagccgatgacagattggaaag +# ctaccaagaaacactggcgactatgaatgtgggaactaaccgactgcttctaagatatcactga] +# protein sequence = [MLSHRHQKAALSKLQRSAYAHYPTPHSPISLTPNRSISSLPASKMLPAVNYVRQADPDAIVLLGHGESYHAFGRVDTN +# LIKGMVSAGNASEILYRDDGLTQWRFKSSRLPHNTSWLVKELNRPVVTLEWNEYATSFLQPLRNDSHWHFLSRTIPDGHSDHEFSVHSESQNIIVIGI +# QGKILKGIKTGEKSQRRVGITCFDPTNGSMSVQGTSLADLRSHIVSFRPRAIVLDSSLIGTYRDIIGTALLGSPEVKFTNMPPVLGSLQLLNDFFDVS +# GEIWRKCFDDYELYSLKAAVEFIKKTQKSTSAVFQLPSRELSSQWTVLLDKETISSLNLGSTGSHYMNSSWLLQRSIKTATVGGSRMLYKSILRPTRI +# VSEILFRQRAVQSIIKRKHTRQKLSSELSSLPDIPLLLQKLKIYPESEDLVSTILHSIDIISSLAPIASKFYMNGHQQDADFDDGDLIYRIPSAKRLE +# ELRDRARSLLNASDTMHGVVSSNTSIECNDRRTQKATLVNGVDCGPNRNTSFSHSVKNQTGDVSLETFMVSDDAELNTESPSNLTTVLDTRTVSEHTQ +# PKGLGEITSSSLDYRDLHATHRIDKLAGQISELHPGHPLPRAKVIANCSAEILRHKELILLFSRLLSRLDMLLSFANVAIQHDDTRPIVDPSRTNFEI +# HLKDKDFNAAADPLLMSNSSSVLWTKGERKTVAELYGLYASIILLAHSGAYVPAKSANIGVVSHMLCQLRSTNPFLLHSALEKSMDGRRIIMCDALPD +# DFEDYGSADDRLESYQETLATMNVGTNRLLLRYH] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g26 +### +# start gene g27 +pf_alpha_strain65 AUGUSTUS gene 7801 8718 0.55 + . ID=g27 +pf_alpha_strain65 AUGUSTUS transcript 7801 8718 0.55 + . ID=g27.t1;Parent=g27 +pf_alpha_strain65 AUGUSTUS start_codon 7801 7803 . + 0 Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS CDS 7801 8715 0.55 + 0 ID=g27.t1.cds;Parent=g27.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 8716 8718 . + 0 Parent=g27.t1 +# coding sequence = [atgattgaagttcctctcggagcacttttggctcttagtgatccatacaccttctcttcacaaggtaaaatgaccggta +# tatcaaagatagtgaagaagcgaaacaaacaaggagttgaggttcttcgtttccgcggatgcccagcgtctaaagtgaggggcacaaggggtgcccaa +# ggactgcatcagtgtatggaaaggaacaattccatgaccagtggagaagcatccgtaacaatttttgctgctacgaagcttcctgatataccaagcat +# accgtatactttacagaagcagctggatgagctccgctctcagacaaccacatcagaccgcaggaagatcaaacgccttccccagctgcaagtgtcgg +# tgaacagagatgatacggttattaaaagggccaccaaaaccaaactaaatgggtttatggctttccgggcatttcattctcgtgaaatttctactatt +# gaacaccaaaggggtatttcgatgtcactggcgactgtgtgggcgagcgatcctccctcgcaacttgtctggcaaagattcgcagcagaatataacca +# aacagtacggtatgagcccgatttgtcatttgttgactggcttctttcaatgtcgaagggggttgactcgaaaattgatgaaccaacaaagagaacct +# taagtagggcagccagggtggagaacatttatgttgagaaggacggaaatctaagacacaacacagatccatatgctcttgaactccaaaataaggac +# gttaacaaccgttatcataactacttgaaggaaagattgcagtcggcaatagatcgtccaattgtcacactcgctagtgacccatcgttatccaggtc +# atatatagatccactagtctatgagatcagtagtggcacttacgaaaaatcctaa] +# protein sequence = [MIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASV +# TIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPP +# SQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDR +# PIVTLASDPSLSRSYIDPLVYEISSGTYEKS] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g27 +### +# start gene g28 +pf_alpha_strain65 AUGUSTUS gene 8788 10017 0.96 - . ID=g28 +pf_alpha_strain65 AUGUSTUS transcript 8788 10017 0.96 - . ID=g28.t1;Parent=g28 +pf_alpha_strain65 AUGUSTUS stop_codon 8788 8790 . - 0 Parent=g28.t1 +pf_alpha_strain65 AUGUSTUS CDS 8791 10017 0.96 - 0 ID=g28.t1.cds;Parent=g28.t1 +pf_alpha_strain65 AUGUSTUS start_codon 10015 10017 . - 0 Parent=g28.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# tcggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacatacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctattatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggcgcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaattga] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g28 +### +# start gene g29 +pf_alpha_strain65 AUGUSTUS gene 10323 10844 1 + . ID=g29 +pf_alpha_strain65 AUGUSTUS transcript 10323 10844 1 + . ID=g29.t1;Parent=g29 +pf_alpha_strain65 AUGUSTUS start_codon 10323 10325 . + 0 Parent=g29.t1 +pf_alpha_strain65 AUGUSTUS CDS 10323 10841 1 + 0 ID=g29.t1.cds;Parent=g29.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 10842 10844 . + 0 Parent=g29.t1 +# coding sequence = [atgaacagcataacccaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g29 +### +# start gene g30 +pf_alpha_strain65 AUGUSTUS gene 11081 12850 0.8 - . ID=g30 +pf_alpha_strain65 AUGUSTUS transcript 11081 12850 0.8 - . ID=g30.t1;Parent=g30 +pf_alpha_strain65 AUGUSTUS stop_codon 11081 11083 . - 0 Parent=g30.t1 +pf_alpha_strain65 AUGUSTUS CDS 11084 12850 0.8 - 0 ID=g30.t1.cds;Parent=g30.t1 +pf_alpha_strain65 AUGUSTUS start_codon 12848 12850 . - 0 Parent=g30.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggacagcacgctgatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcaccaccaggagacttcatccaaggacgagcag +# ctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccgtttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtc +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaatgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgttcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgaaggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g30 +### +# start gene g31 +pf_alpha_strain65 AUGUSTUS gene 13565 14224 0.9 + . ID=g31 +pf_alpha_strain65 AUGUSTUS transcript 13565 14224 0.9 + . ID=g31.t1;Parent=g31 +pf_alpha_strain65 AUGUSTUS start_codon 13565 13567 . + 0 Parent=g31.t1 +pf_alpha_strain65 AUGUSTUS CDS 13565 14221 0.9 + 0 ID=g31.t1.cds;Parent=g31.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 14222 14224 . + 0 Parent=g31.t1 +# coding sequence = [atgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggac +# ctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaag +# gatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagttt +# ggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaagctgccaagtttcgacgttacagagggtacaccattgc +# acagagtcgctgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttaaagtttgatggc +# tgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggt +# tgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNS +# RVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFII +# KSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g31 +### +# start gene g32 +pf_alpha_strain65 AUGUSTUS gene 14368 15765 1 - . ID=g32 +pf_alpha_strain65 AUGUSTUS transcript 14368 15765 1 - . ID=g32.t1;Parent=g32 +pf_alpha_strain65 AUGUSTUS stop_codon 14368 14370 . - 0 Parent=g32.t1 +pf_alpha_strain65 AUGUSTUS CDS 14371 15765 1 - 0 ID=g32.t1.cds;Parent=g32.t1 +pf_alpha_strain65 AUGUSTUS start_codon 15763 15765 . - 0 Parent=g32.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggcgacgctcgaggaagtggtagaggggaaggaagaagtaacagaggtgatagaggtgacagaggcagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtgagtgatcgtgctgatgctactcgtcacacttcgagaagagatgacctgcctcgagatacagccccaactacac +# gccaatcaaggccttcaactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaggagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatttttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccatcttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGEGRSNRGDRGDRGRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADATRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDRSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g32 +### +# start gene g33 +pf_alpha_strain65 AUGUSTUS gene 15970 17838 1 + . ID=g33 +pf_alpha_strain65 AUGUSTUS transcript 15970 17838 1 + . ID=g33.t1;Parent=g33 +pf_alpha_strain65 AUGUSTUS start_codon 15970 15972 . + 0 Parent=g33.t1 +pf_alpha_strain65 AUGUSTUS CDS 15970 17835 1 + 0 ID=g33.t1.cds;Parent=g33.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 17836 17838 . + 0 Parent=g33.t1 +# coding sequence = [atgaattcccaacggcatacaccctatattcgagctgactcctccagtgtgctaccgaggacgctgggccgtcgaccgc +# ccggccctgtcatcccgaacctgggagctaagcaccatcacagctctctcaacaaaccccctcgatcatctcgacagcatgatgaacgagagtcttca +# tcacctcccagaccacacagctcctttgccccacccatcaactccaattcctcaatcacgtccgcctctagtcccaaaaaaaacgttaccgatgggcg +# ggaccccgccaacttgatctccacaacgtatgcctactgcggcaacaggaaacgactgcagaatccaagggaagtcgaaaataaaaagagactaagag +# agatggaagcaaaaaatgctcctggggaaccgccggtgcctctacagtctaaaaggtttcaacaggaagcgtcagttgtcaaggcgaatgatggggac +# acctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctagggagaaacgtgccccaat +# aatgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaactccattcgtgctccaagga +# ttcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagaggtcaaggaaatggatcaa +# caattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcgctgattaaaggtgtgaa +# atcttctgctaagaaaagcacaggatcgacgagtgaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtcagggagtaagtcacctc +# accgccgtggcaccgaagacttgggtattgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttcagtgggggacgctctaggc +# tcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaggaattgcgttccgccaaagagcttctcaaggatgatgaaagtcataaagc +# tatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatcttgagatgatgaagcgca +# ggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatgagtttgaagaggttgac +# gagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacactaagcctcggtgagattgagcaaaa +# ggccaaccagcacatggactgcattccaaaaattctcgacggtcgtcttgactccacttacctacaaaaagcgaaagtattcatgcacgactctggag +# caacaattttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaaggggctattgccaatctg +# atcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattcacattacacattctcgc +# tccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagttgattatggtcaataca +# tcgctgatttggtcccttgtgaatga] +# protein sequence = [MNSQRHTPYIRADSSSVLPRTLGRRPPGPVIPNLGAKHHHSSLNKPPRSSRQHDERESSSPPRPHSSFAPPINSNSSI +# TSASSPKKNVTDGRDPANLISTTYAYCGNRKRLQNPREVENKKRLREMEAKNAPGEPPVPLQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNR +# KRNILSSREKRAPIMSSGEEEQEGSRHRIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRETKKGEDV +# APIPRKRALIKGVKSSAKKSTGSTSEPLGDIRGMLSGKKKSGSKSPHRRGTEDLGIEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGPSSQELR +# SAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNE +# EFPGTLSLGEIEQKANQHMDCIPKILDGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVI +# QFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g33 +### +# start gene g34 +pf_alpha_strain65 AUGUSTUS gene 18296 21421 1 + . ID=g34 +pf_alpha_strain65 AUGUSTUS transcript 18296 21421 1 + . ID=g34.t1;Parent=g34 +pf_alpha_strain65 AUGUSTUS start_codon 18296 18298 . + 0 Parent=g34.t1 +pf_alpha_strain65 AUGUSTUS CDS 18296 21418 1 + 0 ID=g34.t1.cds;Parent=g34.t1 +pf_alpha_strain65 AUGUSTUS stop_codon 21419 21421 . + 0 Parent=g34.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcagaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaccgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgagaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaga +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacctcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYETDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g34 +### +# +# ----- prediction on sequence number 4 (length = 22501, name = pf_alpha_tbrc4498) ----- +# +# Predicted genes for sequence number 4 on both strands +# start gene g35 +pf_alpha_tbrc4498 AUGUSTUS gene 943 1800 0.74 - . ID=g35 +pf_alpha_tbrc4498 AUGUSTUS transcript 943 1800 0.74 - . ID=g35.t1;Parent=g35 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 943 945 . - 0 Parent=g35.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 946 1800 0.74 - 0 ID=g35.t1.cds;Parent=g35.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 1798 1800 . - 0 Parent=g35.t1 +# coding sequence = [atgattagtactaacaatcacaggaaaaaagtggagaatgcgctgattggcctagcctccgctgaagagcagatggaac +# aagctgaacgtgcggcagaaatttcccgtctcaaggccgtttctaagattttcaagacaagaagcgatcacaccaaagttcttgataagttttggcac +# attgttctttctcaacatccagaatttgcagaatacattagggcacaggatttcaagtatatcgagacgattttagatatttatgtctcttgggatgc +# tgcagagtctactgataacggtgttgatcccggtaactttagcataacgtttgacttcaagggaaccgaagatggtgatttccccgcacagattgtga +# cgaagaaattttggatcgagcagattgacccagaagagctcgcagccactgcaagcaagaagaagtcgagtgcaaacttgagtgatgacgaagatgac +# accagtgaagacacagagcgactcgtttctgaagctgctgatatcatctggccaaagtcctatgactctatcaatccatccaagatcacagagaagaa +# gacacctcaggggaagaagaactacagagccggtatgaagagtttctttggcttcttcaagtggacaggacgcaagccgggcaaggaattcccgaacg +# gtgatgatctggctcgactcttcgcagatgatctcttcccaagtgctgtgaagtactacacagaggcacagagggatggtctcgaggaggaagttgac +# tctgatgcgagcgagcccctagatcttagtgaggatgagtccaagggcaccaagagaaaaagggatgacgatgaagatgaagatgagatgtaa] +# protein sequence = [MISTNNHRKKVENALIGLASAEEQMEQAERAAEISRLKAVSKIFKTRSDHTKVLDKFWHIVLSQHPEFAEYIRAQDFK +# YIETILDIYVSWDAAESTDNGVDPGNFSITFDFKGTEDGDFPAQIVTKKFWIEQIDPEELAATASKKKSSANLSDDEDDTSEDTERLVSEAADIIWPK +# SYDSINPSKITEKKTPQGKKNYRAGMKSFFGFFKWTGRKPGKEFPNGDDLARLFADDLFPSAVKYYTEAQRDGLEEEVDSDASEPLDLSEDESKGTKR +# KRDDDEDEDEM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 7 +# RM: 7 +# end gene g35 +### +# start gene g36 +pf_alpha_tbrc4498 AUGUSTUS gene 2147 3073 0.75 - . ID=g36 +pf_alpha_tbrc4498 AUGUSTUS transcript 2147 3073 0.75 - . ID=g36.t1;Parent=g36 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 2147 2149 . - 0 Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 2150 3073 0.75 - 0 ID=g36.t1.cds;Parent=g36.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 3071 3073 . - 0 Parent=g36.t1 +# coding sequence = [atgtcgagcatagcgtccaagactgagagcaccttcagctcctctccatcatccaccacatcccacaaagtcgacgtga +# caccacgccagaagtggcccttctggtatggaggattcggtggtggtgttgcatgtctctttactcatccacttgatctggcaaaagttcgtcttcaa +# accgctccgatcccaaaaccaactcttttccaaatggcaaagtctatcttggttcatgatggtctcttgggtgtttactctggtctctctgcaggtct +# ccttcgtcaggctacttattccctcacaagatttggtgtgtacgagtatttgaaagagaatgttgttcctgctgataagcagtcatctatggcatacc +# tccttcctgtttccatgttgtctggtggtattggtggtgttgttggtaacccagctgatatcgttaacatcagaatgcagaatgatacaggtcttgcc +# ccagatcagagaagaaactacaagcatgccatcgatggtgtcatcagagtggctcgtgaagagggaacttctgctctgttcagaggcctcggaactaa +# cttggtgagaggtgtgctcatgacctcgtcacaggttgtcacttatgatgttgccaaggactggctgatatccttcggtatgaacaaggactctattt +# ccactttcttctccgcttctcttctttcaggtctcgttgctaccaccatttgctccccagcggacgttctcaagaccagaatcatgaactcttctgga +# acaggtgagagcgtcgttcacatcttgaccaccgctttcaaaaacgagggcccttctttcatgtttagaggctgggttccaagtttcgttagattggg +# tccaaacactatcatcacattcttggttgttgagcagctcaagcacttgcgtgttggtatgtga] +# protein sequence = [MSSIASKTESTFSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVH +# DGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAR +# EEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPS +# FMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g36 +### +# start gene g37 +pf_alpha_tbrc4498 AUGUSTUS gene 3734 6130 0.96 + . ID=g37 +pf_alpha_tbrc4498 AUGUSTUS transcript 3734 6130 0.96 + . ID=g37.t1;Parent=g37 +pf_alpha_tbrc4498 AUGUSTUS start_codon 3734 3736 . + 0 Parent=g37.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 3734 6127 0.96 + 0 ID=g37.t1.cds;Parent=g37.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 6128 6130 . + 0 Parent=g37.t1 +# coding sequence = [atgttgtcccaccgccatcagaaagctgctctctccaaactgcaaagaagtgcttacgcacattacccaacaccacatt +# cgccaatatctctaacaccaaatagatcaatatcgtctcttccggcctcgaagatgcttccagctgtcaattacgttcgccaagcagatccagatgcg +# attgttcttctcggtcatggtgaatcttaccatgctttcggcagagtcgatactaatctgattaaaggaatggtatcagctggcaatgcaagcgagat +# actctaccgtgatgatggactgacacaatggagattcaagtcatctcgactcccgcataacacttcttggctggtgaaggagttgaacaggccagttg +# tcaccttggaatggaacgaatatgctactagtttcctacagccattgcgcaacgactcccattggcatttcctatcgagaaccattcctgatggccac +# tcagaccatgaattctctgttcattctgaatctcaaaacatcatagtaattggtatacaggggaagattctcaaggggatcaaaacaggtgaaaagtc +# acagagacgtgttggtatcacttgctttgacccaacaaacggatcgatgtctgttcagggcacttctcttgctgatttgagatcccatatcgtgagct +# ttcgtcctcgggctattgttctggactccagtctgattgggacataccgggacatcattggcacagctcttctaggttcaccagaagttaagtttacc +# aatatgcctcccgtgttgggctcgctacaactccttaatgatttctttgatgtctctggtgaaatttggaggaagtgtttcgatgattacgagctgta +# ctcactgaaggctgctgtggagttcattaagaagacacagaaatcaacgtctgcagtattccaactaccatcgagggaactgtcgtcacaatggaccg +# tgttattggacaaagaaactatcagttccttgaacctgggctccaccgggagtcactatatgaattcctcctggttacttcaaagatcaatcaagact +# gctactgttggtggttctcgaatgttgtacaagtctatcttgaggccaactaggatagtcagtgagattttgtttcgtcagagggcggttcaatccat +# cattaaaaggaaacatacacgtcagaagctgagctcagagctctcgagtttaccagacatcccattgctactacagaaactcaagatctatccggaat +# cagaagaccttgtcagtactatactacactccatcgacatcatctcgtcacttgctcccatcgcctccaagttttacatgaacggtcgccagcaagac +# gctgactttgatgatggtgacttgatttaccgcattccatctgcaaaacgtttggaggaactcagagacagggcccgttcccttctcaatgctagcga +# cacaatgcatggtgttgtttcctcgaatacaagcattgaatgcaatgatcggaggacacagaaagctactttggtgaatggtgttgattgtggaccta +# ataggaacacttctttttctcactctgtgaaaaatcagacaggtgatgtttctttggaaacctttatggtcagcgatgatgctgaattgaacactgag +# tcaccttctaatctcacaacagtgctagacacccgtactgtgagtgaacacacccagcctaaggggctaggagagattaccagctcatctttggacta +# ccgagatctacatgctacacaccgcatcgacaagttggccggtcagataagtgaattacaccctggccaccctctaccaagagccaaagtgattgcca +# actgctcggctgaaatattaagacataaggagctcattctactcttctcccgactcttgtcacgtttggacatgctgctgtcgtttgccaacgtggct +# atccaacatgacgacacaagacctatcgtggatcccagcagaaccaactttgagattcacttgaaggacaaagatttcaatgctgctgctgatccatt +# gttgatgtcaaactcatcatcagtattgtggactaagggagagagaaaaaccgtggcagagctttacgggctgtacgccagtatcatcttattggcgc +# actcaggtgcttatgttccagccaagtctgctaacattggtgttgtcagccacatgctttgtcaactccgctctacaaatcctttcctattacattca +# gcgcttgagaagtctatggatggccgtcgtatcatcatgtgcgacgctctgcctgacgatttcgaagactatggctcagccgatgacagattggaaag +# ctaccaagaaacactggcgactatgaatgtgggaactaaccgactgcttctaagatatcactga] +# protein sequence = [MLSHRHQKAALSKLQRSAYAHYPTPHSPISLTPNRSISSLPASKMLPAVNYVRQADPDAIVLLGHGESYHAFGRVDTN +# LIKGMVSAGNASEILYRDDGLTQWRFKSSRLPHNTSWLVKELNRPVVTLEWNEYATSFLQPLRNDSHWHFLSRTIPDGHSDHEFSVHSESQNIIVIGI +# QGKILKGIKTGEKSQRRVGITCFDPTNGSMSVQGTSLADLRSHIVSFRPRAIVLDSSLIGTYRDIIGTALLGSPEVKFTNMPPVLGSLQLLNDFFDVS +# GEIWRKCFDDYELYSLKAAVEFIKKTQKSTSAVFQLPSRELSSQWTVLLDKETISSLNLGSTGSHYMNSSWLLQRSIKTATVGGSRMLYKSILRPTRI +# VSEILFRQRAVQSIIKRKHTRQKLSSELSSLPDIPLLLQKLKIYPESEDLVSTILHSIDIISSLAPIASKFYMNGRQQDADFDDGDLIYRIPSAKRLE +# ELRDRARSLLNASDTMHGVVSSNTSIECNDRRTQKATLVNGVDCGPNRNTSFSHSVKNQTGDVSLETFMVSDDAELNTESPSNLTTVLDTRTVSEHTQ +# PKGLGEITSSSLDYRDLHATHRIDKLAGQISELHPGHPLPRAKVIANCSAEILRHKELILLFSRLLSRLDMLLSFANVAIQHDDTRPIVDPSRTNFEI +# HLKDKDFNAAADPLLMSNSSSVLWTKGERKTVAELYGLYASIILLAHSGAYVPAKSANIGVVSHMLCQLRSTNPFLLHSALEKSMDGRRIIMCDALPD +# DFEDYGSADDRLESYQETLATMNVGTNRLLLRYH] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 4 +# RM: 4 +# end gene g37 +### +# start gene g38 +pf_alpha_tbrc4498 AUGUSTUS gene 8041 8958 0.67 + . ID=g38 +pf_alpha_tbrc4498 AUGUSTUS transcript 8041 8958 0.67 + . ID=g38.t1;Parent=g38 +pf_alpha_tbrc4498 AUGUSTUS start_codon 8041 8043 . + 0 Parent=g38.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 8041 8955 0.67 + 0 ID=g38.t1.cds;Parent=g38.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 8956 8958 . + 0 Parent=g38.t1 +# coding sequence = [atgattgaagttcctctcggagcacttttggctcttagtgatccatacaccttctcttcacaaggtaaaatgaccggta +# tatcaaagattgtgaagaagcgaaacaaacaaggagttgaggttcttcgtttccgcggatgcccagcgtctaaagtgaggggcacaaggggtgcccaa +# ggactgcatcagtgtatggaaaggaacaattccatgaccagtggagaagcatccgtaacaatttttgctgctacgaagcttcctgatataccaagcat +# accgtatactttacagaagcagctggatgagctccgctctcagacaaccacatcagaccgcaggaagatcaaacgccttccccagctgcaagtgtcgg +# tgaacagagatgatacggttattaaaagggccaccaaaaccaaactaaatgggtttatggctttccgggcatttcattctcgtgaaatttctactatt +# gaacaccaaaggggtatttcgatgtcactggcgactgtgtgggcgagcgatcctccctcgcaacttgtctggcaaagattcgcagcagaatataacca +# aacagtacggtatgagcccgatttgtcatttgttgactggcttctttcaatgtcgaagggggttgactcgaaaattgatgaaccaacaaagagaacct +# taagtagggcagccagggtggagaacatttatgttgagaaggacggaaatctaagacacaacacagatccatatgctcttgaactccaaaataaggac +# gttaacaaccgttatcataactacttgaaggaaagattgcagtcggcaatagatcgtccaattgtcacactcgctagtgacccatcgttatccaggtc +# atatatagatccactagtctatgagatcagtagtggcacttacgaaaaatcctaa] +# protein sequence = [MIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASV +# TIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPP +# SQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDR +# PIVTLASDPSLSRSYIDPLVYEISSGTYEKS] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 1 +# RM: 1 +# end gene g38 +### +# start gene g39 +pf_alpha_tbrc4498 AUGUSTUS gene 9028 10257 0.93 - . ID=g39 +pf_alpha_tbrc4498 AUGUSTUS transcript 9028 10257 0.93 - . ID=g39.t1;Parent=g39 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 9028 9030 . - 0 Parent=g39.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 9031 10257 0.93 - 0 ID=g39.t1.cds;Parent=g39.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 10255 10257 . - 0 Parent=g39.t1 +# coding sequence = [atgggagagaagagatcaagagacgagcaacctgagcttcgtgtttccagcgtcacgaccgatcctacgctggctgtca +# catctttcttcaagggtttctcagttcctgaggatgcacacttcgatctttacaaaaataagaatgatgaagtgatattccacggagagaatgataga +# ctcgagtatgattcgatcacaaacaatgatgacccaagcgactacgccattgccctctacgatccggtgaacaagtccgtcgagctctacaaggctcc +# tgtccttctcggaaacgtcacatctaaggcccacaaaagacttagaggacctaaggtcaaacagaacaacgtccgtgccaacattcaaagaactgctc +# ttggtgaggcctttggtacaaagaaagccaagaaggccatcacagatcttgagagaaatagaattgatgccgagaagctcgtgagtgctgaggccgac +# atcattgacacagtcaagggacacacaacatcgcttcctacaagacaagagctcgagaagactgtactcgacgacagaccaacgcccgttgccgatgt +# tagtgctacatcggttgaagacatctatcctatcgataacatcattccaaagaaggaacaacaattcatccgcgcaggtccaatcatcaaagagaagg +# accttgaaaagagattggagtacatgccataccaggaatcagaatttatcaagaaacagctcgcaggaatcaccgacgactcccagacatccaaacta +# cagctcatctattatgcctctctcctcatgggtgtctaccataacagacgtatctcaaacaagcttgcactcacaacccagctcaactctccaccaga +# ggttcttatcgatggtattattgaccgcttcgctattcaaagacctggccagttcggtcgttccaaggacagaggcttcaagattgatccacatcacg +# aggataagcttctctgtttccttcttgccaccatcatgcacatctccggcttccatgttgaagttgtcccattggcccaagaactttctctgaaaccg +# acaaggcttattggtcttttcaaggcgcttggtgctattgtcaagagcgcaactgttgctgaggctgaggcgttcggtattaagaagaatctcgcttc +# gacttacaaggttgcctctctcaaggtgccattcaagttgccacagatgacaagaggtggtagaagaaattga] +# protein sequence = [MGEKRSRDEQPELRVSSVTTDPTLAVTSFFKGFSVPEDAHFDLYKNKNDEVIFHGENDRLEYDSITNNDDPSDYAIAL +# YDPVNKSVELYKAPVLLGNVTSKAHKRLRGPKVKQNNVRANIQRTALGEAFGTKKAKKAITDLERNRIDAEKLVSAEADIIDTVKGHTTSLPTRQELE +# KTVLDDRPTPVADVSATSVEDIYPIDNIIPKKEQQFIRAGPIIKEKDLEKRLEYMPYQESEFIKKQLAGITDDSQTSKLQLIYYASLLMGVYHNRRIS +# NKLALTTQLNSPPEVLIDGIIDRFAIQRPGQFGRSKDRGFKIDPHHEDKLLCFLLATIMHISGFHVEVVPLAQELSLKPTRLIGLFKALGAIVKSATV +# AEAEAFGIKKNLASTYKVASLKVPFKLPQMTRGGRRN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 9 +# RM: 9 +# end gene g39 +### +# start gene g40 +pf_alpha_tbrc4498 AUGUSTUS gene 10563 11084 1 + . ID=g40 +pf_alpha_tbrc4498 AUGUSTUS transcript 10563 11084 1 + . ID=g40.t1;Parent=g40 +pf_alpha_tbrc4498 AUGUSTUS start_codon 10563 10565 . + 0 Parent=g40.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 10563 11081 1 + 0 ID=g40.t1.cds;Parent=g40.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 11082 11084 . + 0 Parent=g40.t1 +# coding sequence = [atgaacagcataactcaaaagtcgcattacgagcttctcggagtggagccaacagcaagcagcttggagataaaggccg +# catatagagagaaactattatcaactcacccagacaaaacgaaagaggttgtgagtacagatatcattaatcagatcaagcaagcctacatcacactt +# tcggacagcaatctgaggtctgaatatgatgccgatttacaagagtcatttaagaagagtggactcatcagcagtggtgatggtcttgacatattctc +# gctagatgacttcaagtgtgagatgattgggatcggtgaggacgagatggctgtgttcaaaaaggattgtcccagatgcacaactcaagatgggttcg +# agctgaacgaagccgatttggaacaaagtgggacccgagatggtgctggaggctacgagatcattgttcagtgtgctgcctgcagtttatggctcaaa +# gtcaaatactacgaccttgacgatgaagacgacgacaaagatgaggaatga] +# protein sequence = [MNSITQKSHYELLGVEPTASSLEIKAAYREKLLSTHPDKTKEVVSTDIINQIKQAYITLSDSNLRSEYDADLQESFKK +# SGLISSGDGLDIFSLDDFKCEMIGIGEDEMAVFKKDCPRCTTQDGFELNEADLEQSGTRDGAGGYEIIVQCAACSLWLKVKYYDLDDEDDDKDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g40 +### +# start gene g41 +pf_alpha_tbrc4498 AUGUSTUS gene 11321 13090 0.76 - . ID=g41 +pf_alpha_tbrc4498 AUGUSTUS transcript 11321 13090 0.76 - . ID=g41.t1;Parent=g41 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 11321 11323 . - 0 Parent=g41.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 11324 13090 0.76 - 0 ID=g41.t1.cds;Parent=g41.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 13088 13090 . - 0 Parent=g41.t1 +# coding sequence = [atgtcagacaatctccaatcttcagaggccactgaagacaccgagtacttcccaggacagcacgcggatggctcggacg +# tcagctcggatgctgcgcagtccttcatcggtggctcaagtgtagtttcggcacatcctgaacatcaccaccaggagacttcatccaaggacgagcag +# ctggaaaacaaccaggttaccactgatgaccttgagagtagtcattacaagaacgagcacaagattttcacattcagtcttccgtttggtggaaatgc +# gatacacttcccatcaataaagcagattagacagaaccttcctatcattggcagcaacgatacagatgatcgagggtatgaccctactctttcaaggc +# atgagatcaaacagaaactcaagaggcagcagtcggtaagcacgatcgaagaaagttttcttttcaaggacctcaagggtgttgacaacggtaggttc +# agagcagtgaagaaagcattcacgcctgccattagcatcccttcacttatcgacgaaaagaggaagaaggaatccgtgtgggaccagattgaaggtga +# ggtggtaattctgggaggctacagagggagtgtcctcaaggagacggagaccaacaaccgtctctggattccctctttcaagacagggctcaatttga +# acaagaagacattgctgataggcccaaatgatgaggacgaaatcgaagtccagaagcacatatattctccaaagacactaaatcatgttggaccagtc +# gatatcacaaggaaactgagggcgaagctgacaaatggtaagaccaatgtgcatacttttggatatgactggagactgtcctgcgatataagttcaca +# gaaggtgatcgaattcttggccaagttaaaatgcaaccaaggatcgaatcctaaaggtgctattgtcatcgctcactcaatggggggcttggttgccc +# accatgccatgcagcagagaccggatctgttccgtggcttgatctacgtgggtgttccatcaatgtgtccaaacgtgctcggtccaatccgctatggt +# gataccgtgcttttcaacaacaagatcctaacacctgagatcaacttctttatgagatccagttttgtcttcctacctgaggatggccgctgcttcat +# cgacaagaaaacccatgaacagtacaaccttgactattttgatccgcagacttgggtggagtataacttgtcaccagcagtggctaagagccgccaga +# aagccagtgccaaagctgcaaaattggaacctgtcaagtcaccaattttgaaaggcatcgactcttttgacatgaaacgttctctctctttgaggtcc +# tctcacaagtcgacagcagaagaatttaacgtctcatttgacgaatcatacgcatacctctcgcgcacattaaaaagaaccaaaaagttcctcgatga +# ccttaagtacgacccagagaagaaatacccgccacttgcaatcgtttatggtgatcaggttccaactgtgagaggtgcccgtgttgatggtgtcgctg +# atatcaagaatggtaactacgatgacttcttctatggtcctggtgatggtgttgttcatcataagtggttgatgccagagagaagaggattccctgtt +# gcggccaaactttcaagtggtgaaggccacatttcacttatgactgaccttgaaactatgggtgacgctctatttgcagtgctacaggaagagaaagc +# aagactatctgctagagatggataa] +# protein sequence = [MSDNLQSSEATEDTEYFPGQHADGSDVSSDAAQSFIGGSSVVSAHPEHHHQETSSKDEQLENNQVTTDDLESSHYKNE +# HKIFTFSLPFGGNAIHFPSIKQIRQNLPIIGSNDTDDRGYDPTLSRHEIKQKLKRQQSVSTIEESFLFKDLKGVDNGRFRAVKKAFTPAISIPSLIDE +# KRKKESVWDQIEGEVVILGGYRGSVLKETETNNRLWIPSFKTGLNLNKKTLLIGPNDEDEIEVQKHIYSPKTLNHVGPVDITRKLRAKLTNGKTNVHT +# FGYDWRLSCDISSQKVIEFLAKLKCNQGSNPKGAIVIAHSMGGLVAHHAMQQRPDLFRGLIYVGVPSMCPNVLGPIRYGDTVLFNNKILTPEINFFMR +# SSFVFLPEDGRCFIDKKTHEQYNLDYFDPQTWVEYNLSPAVAKSRQKASAKAAKLEPVKSPILKGIDSFDMKRSLSLRSSHKSTAEEFNVSFDESYAY +# LSRTLKRTKKFLDDLKYDPEKKYPPLAIVYGDQVPTVRGARVDGVADIKNGNYDDFFYGPGDGVVHHKWLMPERRGFPVAAKLSSGEGHISLMTDLET +# MGDALFAVLQEEKARLSARDG] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g41 +### +# start gene g42 +pf_alpha_tbrc4498 AUGUSTUS gene 13805 14464 0.93 + . ID=g42 +pf_alpha_tbrc4498 AUGUSTUS transcript 13805 14464 0.93 + . ID=g42.t1;Parent=g42 +pf_alpha_tbrc4498 AUGUSTUS start_codon 13805 13807 . + 0 Parent=g42.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 13805 14461 0.93 + 0 ID=g42.t1.cds;Parent=g42.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 14462 14464 . + 0 Parent=g42.t1 +# coding sequence = [atgtggacattcccacactactactacttcaagaagggttccctcgccgaacgtcgtttcttcaaggcacagcgtggac +# ctgtatctaagcagccaggtgtcttctactcgaaaggtgtgcctgatatcgtgcacaaccgtgaacgtcgctcgaagcaggaggttgtgattccaaag +# gatgaatacgaagaatccgcaatgtcggacatttcgagaccagttttaccaaactctcgtgttacaaaagcagacgaacagggtgatttgacaagttt +# ggaaagacagctaccgagaacactttatcttcttgtaaaggatggaaagcttggctggaaactgccaagtttcgacgttacagagggtacaccattgc +# acagagtcgctgaggacggtttaagacaacttggtggtgaccagattaacacatggactgtcgctcgttctccaacggcagtcttaaagtttgatggc +# tgctctttggctccagtggatgctcagaagaagggtctctccagagaattcatcattaagtccaatatcctctcaggtgcattcaagccacagaaggt +# tgagtatgcttggttgactcgtgaggagattgaagagaaggtgagtggggaatacttccaggcaactgagtttttgttctctcgtgtctga] +# protein sequence = [MWTFPHYYYFKKGSLAERRFFKAQRGPVSKQPGVFYSKGVPDIVHNRERRSKQEVVIPKDEYEESAMSDISRPVLPNS +# RVTKADEQGDLTSLERQLPRTLYLLVKDGKLGWKLPSFDVTEGTPLHRVAEDGLRQLGGDQINTWTVARSPTAVLKFDGCSLAPVDAQKKGLSREFII +# KSNILSGAFKPQKVEYAWLTREEIEEKVSGEYFQATEFLFSRV] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 2 +# RM: 2 +# end gene g42 +### +# start gene g43 +pf_alpha_tbrc4498 AUGUSTUS gene 14608 16005 1 - . ID=g43 +pf_alpha_tbrc4498 AUGUSTUS transcript 14608 16005 1 - . ID=g43.t1;Parent=g43 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 14608 14610 . - 0 Parent=g43.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 14611 16005 1 - 0 ID=g43.t1.cds;Parent=g43.t1 +pf_alpha_tbrc4498 AUGUSTUS start_codon 16003 16005 . - 0 Parent=g43.t1 +# coding sequence = [atgtccactctaagaagatctgataggggtgctgatgacagacttggccgaggtgttcgtggtgatcgaggtcatgaaa +# gaggtgacgctcgaggaagtggtagaggggatggaagaggtaacagaggtgatagaggtgacagaaacagggatctccgggaccgggaaagagaaaga +# gacagagacagaccctcaagggatgatcgtgatagagacggccatggcacgagagacactggtagaccgaatgaccgtggagcaatccgcggaggaga +# taaagcttcggaccgtaaccgtgtaagtgatcgtgctgatgctagtcgtcacacatcgagaagagacgacctgcctcgagatacagccccaactacac +# gccaatcaaggccctctactagcaagaaatcgaagacggtgcttccatcgggaccaagccaacagccgttggaaaagatgaatcatatagagcttgtc +# aaaccaaatcgtgaaactcaccgtggcttcgaaataactgctgctgaatggagttacaacggtgcacgtattgctgtgtcacgtacagatggtatact +# cgttatatggaacgtgaacaagaacaatggtgatccaacaccaagacagccaaccgttatgaagaaattacatgaaggtatggtaacacgaattgcat +# ggagaccagaaagcgatgagattttagccacaattggaaaggacaagagcattcatatttggaacattgtgactggtaagctaatgcatgagattgag +# accgcagagacaacaccagttgatattgcatactctccgaatgcagaatacttggctgtcatcacacaagcacacacacttgtgatctataatgctaa +# cacatacgaacagttgcagaccttagagtatggaaaggatggcgtatcgtcgctttgttggagcaacaactccgtggccatatttgtcggtcttgtga +# ctggtaagatctctgttgttatcaagggcggtgatggtgcctttgtcaatgcttgcatacttgagggtcaccgttctaggatcaattccctgtgtatg +# gacccacggggtcgctacatcgctgctggttctcacgagggtatcatctcactctggtccaccacagacctaacattggcccgaaccattggaactgt +# tgatcagtcaattcatagaatggatctctttgctgatggcgtccaccttggtgtgacttatcaagaggcagactctgcgaagatcttcaacatagaga +# ccggtaaagaggtcgtgtcgctcccaggatgtaattctggaactaatctcgatggaaagttcagattcagcccagtggcaatctcatatctttacaca +# aatggtgatgggtctgttttcactggtgttgaacgtaccaattga] +# protein sequence = [MSTLRRSDRGADDRLGRGVRGDRGHERGDARGSGRGDGRGNRGDRGDRNRDLRDRERERDRDRPSRDDRDRDGHGTRD +# TGRPNDRGAIRGGDKASDRNRVSDRADASRHTSRRDDLPRDTAPTTRQSRPSTSKKSKTVLPSGPSQQPLEKMNHIELVKPNRETHRGFEITAAEWSY +# NGARIAVSRTDGILVIWNVNKNNGDPTPRQPTVMKKLHEGMVTRIAWRPESDEILATIGKDKSIHIWNIVTGKLMHEIETAETTPVDIAYSPNAEYLA +# VITQAHTLVIYNANTYEQLQTLEYGKDGVSSLCWSNNSVAIFVGLVTGKISVVIKGGDGAFVNACILEGHRSRINSLCMDPRGRYIAAGSHEGIISLW +# STTDLTLARTIGTVDQSIHRMDLFADGVHLGVTYQEADSAKIFNIETGKEVVSLPGCNSGTNLDGKFRFSPVAISYLYTNGDGSVFTGVERTN] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 3 +# RM: 3 +# end gene g43 +### +# start gene g44 +pf_alpha_tbrc4498 AUGUSTUS gene 16210 18078 1 + . ID=g44 +pf_alpha_tbrc4498 AUGUSTUS transcript 16210 18078 1 + . ID=g44.t1;Parent=g44 +pf_alpha_tbrc4498 AUGUSTUS start_codon 16210 16212 . + 0 Parent=g44.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 16210 18075 1 + 0 ID=g44.t1.cds;Parent=g44.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 18076 18078 . + 0 Parent=g44.t1 +# coding sequence = [atgaattcccaacggcatacaccctatattcgagctgactcctccagtgtgctaccgaggacgctgggccgtcgaccgc +# ccggccctgtcatcccgaacctgggagctaagcaccatcacagctctctcaacaaaccccctcgatcatctcgacagcatgatgaacgagagtcttca +# tcacctcccagaccacacagctcctttgccccacccatcaactccaattcctcaatcacgtccgcctctagtcccaaaaaaaacgttaccgatgggcg +# ggaccccgccaacttgatctccacaacgtatgcctactgcggcaacaggaaacgactgcagaatccaagggaagtcgaaaataaaaagagactaagag +# agatggaagcaaaaaatgctcctggggaaccgccggtgcctctacagtctaaaaggtttcaacaggaagcgtcagttgtcaaggcgaatgatggggac +# acctttgagcctctaacgtcttcgtctggtgtgctatcacaagagacaaacaaccgaaaaagaaacatactttcttctttggagaaacgtgccccaat +# aatgtcttcaggtgaagaggaacaagaaggttcaaggcatagaattccttcaattcaatcagacgctgacttcatgaactccattcgtgctccaagga +# ttcccagtcgagctgctcaaactgactacgagaagcacgctgctagcgctcgtctacaacataaacgtgaggatacagaggtcaaggaaatggatcaa +# caattgcatgatgacacgaattcagggaaaagggaaactaaaaaaggggaagatgtcgctccaataccaaggaagagggcgctgattaaaggtgtgaa +# atcttctgctaagaaaagcacaggatcgacgagtcaaccattgggtgatattaggggcatgctgtctggaaagaaaaagtcagggagtaagtcacctc +# accgccgtggcaccgaagacttgggtactgaagatccaggtggtgagacacacaagcaccgggaccgtcatgaaggttcagtgggggacgctctaggc +# tcggatgaagagttgttcaacttcgactcgcctgggccatcctcgcaagaattgcgttccgccaaagagcttctcaaggatgatgaaagtcataaagc +# tatgaagtctgcaattgacagtctctcaataaaaagcggcatattgaacaaacgattcaaacccgatggtgatgactatcttgagatgatgaagcgca +# ggtctgtctttgacccaaagcagtctcgaaacattgagtacgctgacttgaatcaggatatcagtgttggtctacaaaatgagtttgaagaggttgac +# gagcaggcaaacgaggtcaaacagcaagctattgtgaaaaggaagtatggaaatgaggagttcccgggaacattaagcctcggtgagattgagcaaaa +# ggccaaccagcacatggactgcattccaaaaattctcaacggtcgtcttgactccacttacctacaaaaagcgaaagtattcatgcacgactctggag +# caacaatcttgtccaatgcagatctccaaagacctagtttcaagcagttctttagtggattttatggtttgaagaggcaaggggctattgccaatctg +# atcagaagaaagtaccataaacttatcaaggagaaggccgagcacaacagggttatccaattttggtccatcgatacattcacattacacattctcgc +# tccagaggttgctgcaagaatggccgccgaggatatgagtatcagcatcgatgaggctatcagagtgttggatagaacagttgattatggtcaataca +# tcgctgatttggtcccttgtgaatga] +# protein sequence = [MNSQRHTPYIRADSSSVLPRTLGRRPPGPVIPNLGAKHHHSSLNKPPRSSRQHDERESSSPPRPHSSFAPPINSNSSI +# TSASSPKKNVTDGRDPANLISTTYAYCGNRKRLQNPREVENKKRLREMEAKNAPGEPPVPLQSKRFQQEASVVKANDGDTFEPLTSSSGVLSQETNNR +# KRNILSSLEKRAPIMSSGEEEQEGSRHRIPSIQSDADFMNSIRAPRIPSRAAQTDYEKHAASARLQHKREDTEVKEMDQQLHDDTNSGKRETKKGEDV +# APIPRKRALIKGVKSSAKKSTGSTSQPLGDIRGMLSGKKKSGSKSPHRRGTEDLGTEDPGGETHKHRDRHEGSVGDALGSDEELFNFDSPGPSSQELR +# SAKELLKDDESHKAMKSAIDSLSIKSGILNKRFKPDGDDYLEMMKRRSVFDPKQSRNIEYADLNQDISVGLQNEFEEVDEQANEVKQQAIVKRKYGNE +# EFPGTLSLGEIEQKANQHMDCIPKILNGRLDSTYLQKAKVFMHDSGATILSNADLQRPSFKQFFSGFYGLKRQGAIANLIRRKYHKLIKEKAEHNRVI +# QFWSIDTFTLHILAPEVAARMAAEDMSISIDEAIRVLDRTVDYGQYIADLVPCE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 8 +# RM: 8 +# end gene g44 +### +# start gene g45 +pf_alpha_tbrc4498 AUGUSTUS gene 18536 21661 1 + . ID=g45 +pf_alpha_tbrc4498 AUGUSTUS transcript 18536 21661 1 + . ID=g45.t1;Parent=g45 +pf_alpha_tbrc4498 AUGUSTUS start_codon 18536 18538 . + 0 Parent=g45.t1 +pf_alpha_tbrc4498 AUGUSTUS CDS 18536 21658 1 + 0 ID=g45.t1.cds;Parent=g45.t1 +pf_alpha_tbrc4498 AUGUSTUS stop_codon 21659 21661 . + 0 Parent=g45.t1 +# coding sequence = [atgtcgaagacaatctccgactctgaccttatttcatcgttgaagaaggctacttctctcgacgaaactgctccaaaga +# gaaaacacgtcagagcatgtattgtctacacctgggaccacaagtcctccagacagttcttcaaccagctgaaactgctccctgttcagggtgatgaa +# gttcaactctttaagacactgatcaccattcacaaggttctccaggagggtcatggctctgctttgaaggaggccatgcgtaatgctgattggatcga +# gggcctaggaagatcagtccatggtgatggattcaagggctacggaagactcatcaaggaatacgtggtctatttgttaagaaagctcaactttcata +# agaaccaccgtggattcaatggtacattcgagtatgaagagtatgtctcccttgttaccgtgcaaaaccctgacgaaggttacgaggccattctcgat +# ctcatgactttgcaagatgctctggacgatcttcaaagactgatctttgctagtatctcccatgacagaaagtctgagtgtaagatctctgctcttgt +# gccattggttgctgagtcatacggtatctacaagttcattacgtctatgctcagggctattcaccgctccacgggatctgatgaggcattggaacctt +# taagagaacgctatgagtctcaacatgccagactctacgagttctatgctgactgctcctcaatccgctacttgacttccttgattacaatcccaaga +# cttccagaaacagcaccaaacctcatctccaaagatgagagtgacgtggaaagtgaaagaccggcctcaagacaaacttcggtcgctacaccagttcc +# agaaccaatctcggaacagccaactatgggtaacttccaacagccaattgcatctcagccaactggtgttgatttctggaacacccaacaaaacgtgt +# ttgaagccgaacagcagagacttgcaagagaagcccaagaaaacatgcttcaacagcagcaacaggctgagcaacagcgtatttggtttgaacagcaa +# caggctgctcaggctgaacagcagaggctagcccaggaacagcttttgcgcgatcagttgcagaaccaggcagagggaagagtcgcagagctagaacg +# tgatttgctcgcaatgaggcaacagtacgagaacgatcagttgttattacagcagtacgatcaaagagtcaagaccctggagcaggaaattgccacca +# caacacagactgctactcagcaacttggttctaaggatgagcttttgaacagtttgcaggaacagttgactttatggaaaaataagtacgattctttg +# gctaagctgtactcacagttgagacaggagcatttgaacctgttgaacaagtttaagaagttacagcaaaaggctgcctcggcccaggaagccatcga +# gcgtcgtgagaagcttgaaaaggacttgaagtctaagaacgttgagctggcaggtctgatcagagaacgtgacagagctagattggacttagagaaaa +# ttaagggtggtaaagacagcgagattgataagttggagctccagatcagggacttgacattgaagcttgatgactctgagagaagccagtcctcgaac +# ttatcgtcggtgtttgcttctcacaagagagagatggaagacttgaaggcaaaacttgagacacagtctcgtgctctgaacccagaccttgaacagaa +# gctcaaagagaaggaagaggagcttgagattgtgcagcaaaccatggatgatgcccttcaggagcttgccagagtccagggagagaacgatgaggcta +# ttaacgaacaacttgatgcagtcctgattgatcacctcactaagctcacatcattggttgatgccatcttggcttccggtatcaaaaggattcaggac +# tccttgtacgagctggactctccaatgcaggctggtaatcaaaactcatcaccggagtacttgctttccatcatcgagaaggcccaatcgtccgctac +# cgactttgccaactctttcaatgacttcattgccgatggtccaaatggtgatcactctgagatcatcagatccattaacgaattcacatcttctatcg +# ccgatgttctgctcaatgccaagggtattgtgagacttgtgaagactgatagtgaatctaatcagctcattgccgcctccagatctaccgctcaggaa +# tctgaggacttccttatgtctctcctctccgatatccttgaaggaactgaggaagagaaaaccgaagttgttatttccggtaacttgaacgtccaaga +# gaagcttcaagaactcattgttattgctgagaaccttgcaccaagatctgttctcaagaacattaatggtgatcttggtgatgctgttgataacgaat +# tgaactctgccgcttctgctatcacatctgctgctgctcatctcaaggatctgcttaactcctctttcgattcatccatcgacggtgatatcaacaag +# gccatcttatctgctgctatttccgttaccaactcaatcgctcttttgattcaggccgccattgagacacagaatgagattgtgacgaaaaatcaggg +# aagtggtacccgtgcccagttctacaagaaacacaatagatggactgagggtctcatttcagctgctaaggctgttgctgcctccaccaagatcttga +# ttggtactgcggatggtgtgttgagttctaagaacacccatgaagaattgattgtggcttccaatgaagtggctgcttccactgcccaacttgttgct +# gcctcaagagttaaggctacattcatgtccaagacgcaggatacgttggagaatgcttccaagactgttacttctgcctgcagatctttggtcaacca +# agttcaggatattctgaaccagagaacgaatggtggtgatcaggatattgacttctcgaagttgacgaaccatgagaacaagactatggagatggagc +# agcaagtggagattttgaagttggagaatgccttggcttccgctagaaagagattgggtgagattagaaagtatggatacagagatggtgacagtgat +# gaagagtaa] +# protein sequence = [MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHG +# SALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASI +# SHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESE +# RPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQ +# LQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFK +# KLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKL +# ETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPE +# YLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEE +# KTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQA +# AIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENA +# SKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE] +# Evidence for and against this transcript: +# % of transcript supported by hints (any source): 0 +# CDS exons: 0/1 +# CDS introns: 0/0 +# 5'UTR exons and introns: 0/0 +# 3'UTR exons and introns: 0/0 +# hint groups fully obeyed: 0 +# incompatible hint groups: 19 +# RM: 19 +# end gene g45 +### +# command line: +# augustus --species=saccharomyces_cerevisiae_S288C --genemodel=complete --strand=both --protein=on --codingseq=on --gff3=on windows.fa diff --git a/results/2026-10-30_petasospora/data/ena_runs.tsv b/results/2026-10-30_petasospora/data/ena_runs.tsv new file mode 100644 index 00000000..c815b4b9 --- /dev/null +++ b/results/2026-10-30_petasospora/data/ena_runs.tsv @@ -0,0 +1,26 @@ +run_accession scientific_name library_strategy library_layout read_count study_accession strain library_source +DRR032607 Petasospora fabianii WGS PAIRED 1265792 PRJDB3616 JCM 3601 GENOMIC +SRR22512789 Petasospora fabianii WGS PAIRED 8154510 PRJNA907923 CYCFB003 GENOMIC +SRR11058199 Petasospora fabianii WGS SINGLE 90107 PRJNA605184 GENOMIC +SRR11058200 Petasospora fabianii WGS PAIRED 4019443 PRJNA605184 GENOMIC +SRR16988870 Petasospora fabianii WGS PAIRED 2736785 PRJNA736342 NRRL Y-1871 GENOMIC +SRR30295406 Petasospora fabianii WGS PAIRED 13416713 PRJNA1122128 BB21_4 GENOMIC +SRR33537244 Petasospora fabianii WGS PAIRED 4333594 PRJNA1257546 TBRC4498 GENOMIC +DRR032482 Petasospora fabianii WGS PAIRED 6566478 PRJDB3616 JCM 3601 GENOMIC +SRR5047279 Petasospora fabianii WGS SINGLE 91709 PRJNA353175 65 GENOMIC +ERR11011131 Petasospora fabianii WGS SINGLE 232809 PRJEB57332 GENOMIC +SRR22512788 Petasospora fabianii WGS PAIRED 10808623 PRJNA907923 CYCFB004 GENOMIC +SRR22512790 Petasospora fabianii WGS PAIRED 8849354 PRJNA907923 CYCFB002 GENOMIC +SRR33624606 Petasospora fabianii AMPLICON SINGLE 1 PRJNA1185440 missing METAGENOMIC +SRR30295292 Petasospora fabianii WGS PAIRED 15665115 PRJNA1122128 BB21_3 GENOMIC +SRR30295294 Petasospora fabianii WGS PAIRED 15773221 PRJNA1122128 BB21_1 GENOMIC +SRR5047278 Petasospora fabianii WGS PAIRED 1992031 PRJNA353175 65 GENOMIC +SRR10913294 Petasospora fabianii WGS PAIRED 2530450 PRJNA517730 Ex2 GENOMIC +SRR11058201 Petasospora fabianii WGS PAIRED 4236935 PRJNA605184 GENOMIC +SRR12907801 Petasospora fabianii WGS PAIRED 2736785 PRJNA647756 NRRL Y-1871 GENOMIC +SRR22512791 Petasospora fabianii WGS PAIRED 7501745 PRJNA907923 CYCFB001 GENOMIC +SRR30295293 Petasospora fabianii WGS PAIRED 16335104 PRJNA1122128 BB21_2 GENOMIC +SRR33537245 Petasospora fabianii WGS PAIRED 3575433 PRJNA1257546 TBRC4498 GENOMIC +SRR30295405 Petasospora fabianii WGS PAIRED 18920264 PRJNA1122128 BB21_5 GENOMIC +ERR17672359 Petasospora fabianii WGS PAIRED 4641284 PRJEB123122 GENOMIC +SRR39042100 Petasospora fabianii WGS SINGLE 131501 PRJNA1475924 HLS091 GENOMIC diff --git a/results/2026-10-30_petasospora/data/extra_refs.faa b/results/2026-10-30_petasospora/data/extra_refs.faa new file mode 100644 index 00000000..cb1955be --- /dev/null +++ b/results/2026-10-30_petasospora/data/extra_refs.faa @@ -0,0 +1,11 @@ +>ONH67106.1 Mating-type-like protein ALPHA1 [Petasospora fabianii] +MTHDVLSQYIKNHSLVSSFNGGMKVHDVIVEALSFIKEELCFATQYFNITQLILEASQFSLREAMKPLDL +QVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPPSQLVWQRFAAEYNQT +VRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYH +NYLKERLQSAIDRPIVTLASDPSLSRSYIDPLVYEISSGTYEKS + +>XP_020071302.1 HMG-box domain-containing protein CYBJADRAFT_71620 [Williopsis jadinii] +MSKQRMLAKVVLNDAKHTISALSQVKVTPPVFTREYNDMGNGRSEIIKSLIDDLVKDISRVSIGIQALQV +NLNLDVSPIQFSTRPVRKKTLYNTWYKDNFSNCKEEHPEWDATRITKKLVRDWREFSDDDKEALEIEYGF +KSKTE + diff --git a/results/2026-10-30_petasospora/data/models.tsv b/results/2026-10-30_petasospora/data/models.tsv new file mode 100644 index 00000000..8489aacb --- /dev/null +++ b/results/2026-10-30_petasospora/data/models.tsv @@ -0,0 +1,15 @@ +record contig gene strand exons cds_nt aa start stop_in_frame_end intron_signals +MATa:JOY008 CP048715.1 dic1 - 628452-629375 924 308 M(ATG) stop_after +MATa:JOY008 CP048715.1 MATa2 - 630492-630863;629853-630419 939 313 M(ATG) stop_after 630420-630491(72bp):GTAGGT..ATCTAG +MATa:JOY008 CP048715.1 MATa1 + 631437-631737;631835-632133 600 200 M(ATG) stop_after 631738-631834(97bp):GTACCA..CGAAAG +MATa:JOY008 CP048715.1 sla2 + 641746-644868 3123 1041 M(ATG) stop_after +MATa:JCM3601 BCGI01000001.1 dic1 - 641150-642073 924 308 M(ATG) stop_after +MATa:JCM3601 BCGI01000001.1 MATa2 - 643190-643561;642551-643117 939 313 M(ATG) stop_after 643118-643189(72bp):GTAGGT..ATCTAG +MATa:JCM3601 BCGI01000001.1 MATa1 + 644135-644435;644533-644831 600 200 M(ATG) stop_after 644436-644532(97bp):GTACCA..CGAAAG +MATa:JCM3601 BCGI01000001.1 sla2 + 654441-657563 3123 1041 M(ATG) stop_after +MATalpha:TBRC4498 CM115473.1 dic1 - 631649-632572 924 308 M(ATG) stop_after +MATalpha:TBRC4498 CM115473.1 MATalpha1 + 637540-638454 915 305 M(ATG) stop_after +MATalpha:TBRC4498 CM115473.1 sla2 + 648035-651157 3123 1041 M(ATG) stop_after +MATalpha:strain65 MPUK01000005.1 dic1 - 611410-612333 924 308 M(ATG) stop_after +MATalpha:strain65 MPUK01000005.1 MATalpha1 + 617300-618214 915 305 M(ATG) stop_after +MATalpha:strain65 MPUK01000005.1 sla2 + 627795-630917 3123 1041 M(ATG) stop_after diff --git a/results/2026-10-30_petasospora/data/models_proteins.faa b/results/2026-10-30_petasospora/data/models_proteins.faa new file mode 100644 index 00000000..d2999c3a --- /dev/null +++ b/results/2026-10-30_petasospora/data/models_proteins.faa @@ -0,0 +1,28 @@ +>MATa_JOY008|dic1|CP048715.1:628452-629375(-) +MSSIASKTESTLSSSPSSTTSHKVDVTARQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVHDGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAREEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILSTAFKNEGPSFMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM +>MATa_JOY008|MATa2|CP048715.1:629853-630863(-) +MVTIGAQCCRNGEAPPSISTFPILSKISFTLTKTDLPTKVFWEKKQFPVASRLAAGANAPRSPKEITFAHLSSGTSTKSIYVYAPHNNVARPRNKFIIARTILSRIVKTRHPWSTNDVSKIISKIWGSGNRLFLSYFDYLSFLENESHECRYPLYHYNPRSRSTVAKASACTASAKNSELEQNMSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAPVDSASTNARTTYKVTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM +>MATa_JOY008|MATa1|CP048715.1:631437-632133(+) +MATRDQIAYKEASEKLDALIEFSSNFSTNPTKPRYDKIMLEDPFEKDSLLSKVPAYARAALETLISNFELSLGRVMVGQSLSHKSNQSVIKTNKPLGPRLKDPEAYGHRIASYEVQSTTIEVTGSTSSPVFPGGGRKRNALPAKKRTSITPESKEFLERVFKAKRTPNSTERKVIAEKCGLTPLQVRVWFTNKRMRNKEG +>MATa_JOY008|sla2|CP048715.1:641746-644868(+) +MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHGSALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASISHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESERPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQLQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFKKLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKLETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPEYLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEEKTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAIAVTNSIALLIQAAIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENASKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE +>MATa_JCM3601|dic1|BCGI01000001.1:641150-642073(-) +MSSIASKTESTLSSSPSSTTSHKVDVAPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVHDGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAREEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPSFMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM +>MATa_JCM3601|MATa2|BCGI01000001.1:642551-643561(-) +MVTIGAQCCRNGEAPPSISTFPILSKISFTLTKTDLPTKVFWEKKQFPVASPLAAGANAPRSPKEITFAHLSSGTSTKSIYVYAPHNNVARPRNKFIIARTILSRIVKTRHPWSTNDVSKIISKIWGSGNRLFLSYFDYLSFLENESHECRYPLYHYNPRSRSTVAKASACTASAKNSELEQNMSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAPVGSASTNARTTYKVTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM +>MATa_JCM3601|MATa1|BCGI01000001.1:644135-644831(+) +MATRDQIAYKEASEKLDALIEFSSNFSTNPTKPRYDKIMLEDPFEKESLLSKVPAYARAALETLISNFELSLGRVMVGQSLSHKSNQSVIKTNKPLGPRLKDPEAYGHRIASYEVQSTTIEVTGSTSSPVFPGGGRKRNALPAKKRTSITPESKEFLERVFKAKRTPNSTERKVIAEKCGLTPLQVRVWFTNKRMRNKEG +>MATa_JCM3601|sla2|BCGI01000001.1:654441-657563(+) +MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHGSALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASISHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESERPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQLQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFKKLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKLETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPEYLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEEKTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQAAIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENASKTVTSACRSLVNQVQDILNQRTNGGDQDVDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE +>MATalpha_TBRC4498|dic1|CM115473.1:631649-632572(-) +MSSIASKTESTFSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVHDGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAREEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPSFMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM +>MATalpha_TBRC4498|MATalpha1|CM115473.1:637540-638454(+) +MIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASVTIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPPSQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDRPIVTLASDPSLSRSYIDPLVYEISSGTYEKS +>MATalpha_TBRC4498|sla2|CM115473.1:648035-651157(+) +MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHGSALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASISHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESERPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQLQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFKKLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKLETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPEYLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEEKTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQAAIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENASKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE +>MATalpha_strain65|dic1|MPUK01000005.1:611410-612333(-) +MSSIASKTESTLSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVHDGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAREEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPSFMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM +>MATalpha_strain65|MATalpha1|MPUK01000005.1:617300-618214(+) +MIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASVTIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPPSQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDRPIVTLASDPSLSRSYIDPLVYEISSGTYEKS +>MATalpha_strain65|sla2|MPUK01000005.1:627795-630917(+) +MSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHGSALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASISHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESERPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQLQNQAEGRVAELERDLLAMRQQYETDQLLLQQYDQRVKTLEQEIATTTQTATQQLGSKDELLNSLQEQLTLWKNKYDSLAKLYSQLRQEHLNLLNKFKKLQQKAASAQEAIERREKLEKDLKSKNVELAGLIRERDRARLDLEKIKGGKDSEIDKLELQIRDLTLKLDDSERSQSSNLSSVFASHKREMEDLKAKLETQSRALNPDLEQKLKEKEEELEIVQQTMDDALQELARVQGENDEAINEQLDAVLIDHLTKLTSLVDAILASGIKRIQDSLYELDSPMQAGNQNSSPEYLLSIIEKAQSSATDFANSFNDFIADGPNGDHSEIIRSINEFTSSIADVLLNAKGIVRLVKTDSESNQLIAASRSTAQESEDFLMSLLSDILEGTEEEKTEVVISGNLNVQEKLQELIVIAENLAPRSVLKNINGDLGDAVDNELNSAASAITSAAAHLKDLLNSSFDSSIDGDINKAILSAAISVTNSIALLIQAAIETQNEIVTKNQGSGTRAQFYKKHNRWTEGLISAAKAVAASTKILIGTADGVLSSKNTHEELIVASNEVAASTAQLVAASRVKATFMSKTQDTLENASKTVTSACRSLVNQVQDILNQRTNGGDQDIDFSKLTNHENKTMEMEQQVEILKLENALASARKRLGEIRKYGYRDGDSDEE diff --git a/results/2026-10-30_petasospora/data/scan_a_cp048715.tsv b/results/2026-10-30_petasospora/data/scan_a_cp048715.tsv new file mode 100644 index 00000000..13e9e883 --- /dev/null +++ b/results/2026-10-30_petasospora/data/scan_a_cp048715.tsv @@ -0,0 +1,6 @@ +frag strand start end aa pfam best_ref bits seq ++0_631726-632133 + 631726 632133 136 [('Homeodomain', 65.5), ('Homeobox_KN', 30.0)] 460519_cbs-7435_MATkp_MATa_MATa1 69 DHDSVPRETIFKTYKRILEREVDRALVDGILSIPPKEDPEAYGHRIASYEVQSTTIEVTGSTSSPVFPGGGRKRNALPAKKRTSITPESKEFLERVFKAKRTPNSTERKVIAEKCGLTPLQVRVWFTNKRMRNKEG ++0_641728-642999 + 641728 642999 424 [('ANTH', 253.9)] sla2 498 LTILEKMSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHGSALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASISHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESERPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQLQNQAEGRVAELERDLLAMRQQYENDQLLLQQYDQRVKTLEQEIAT +-2_630435-630908 - 630435 630908 158 [] MTLA2 24 NFSIDREEYPYPSFKMVTIGAQCCRNGEAPPSISTFPILSKISFTLTKTDLPTKVFWEKKQFPVASRLAAGANAPRSPKEITFAHLSSGTSTKSIYVYAPHNNVARPRNKFIIARTILSRIVKTRHPWSTNDVSKIISKVGTFSMNFSHTHSISTVEY +-2_629853-630419 - 629853 630419 189 [] MATA 29 IWGSGNRLFLSYFDYLSFLENESHECRYPLYHYNPRSRSTVAKASACTASAKNSELEQNMSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAPVDSASTNARTTYKVTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM +-2_628452-629429 - 628452 629429 326 [] dic1 374 RARALSSPHHPNHITPHAMSSIASKTESTLSSSPSSTTSHKVDVTARQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVHDGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMLSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAREEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILSTAFKNEGPSFMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM diff --git a/results/2026-10-30_petasospora/data/scan_alpha_strain65.tsv b/results/2026-10-30_petasospora/data/scan_alpha_strain65.tsv new file mode 100644 index 00000000..ce0029ec --- /dev/null +++ b/results/2026-10-30_petasospora/data/scan_alpha_strain65.tsv @@ -0,0 +1,7 @@ +frag strand start end aa pfam best_ref bits seq ++0_616049-616426 + 616049 616426 126 [] ONH67106.1 139 WLYLHRSISISAVSGKEKKKILTDLPLFKYLYFFFLQSLLFDSLCLPMTHDVLSQYIKNHSLVSSFNGGMKVHDVIVEALSFIKEELCFATQYFNITQLILEASQFSLREAMKPLDVLSSMLESTR ++0_617231-618214 + 617231 618214 328 [('MATalpha_HMGbox', 36.3)] ONH67106.1 407 HDWCFEATVCIVSGQEISVVRRRMIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASVTIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPPSQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDRPIVTLASDPSLSRSYIDPLVYEISSGTYEKS ++1_627777-629000 + 627777 629000 408 [('ANTH', 254.0)] sla2 476 LTILEKMSKTISDSDLISSLKKATSLDETAPKRKHVRACIVYTWDHKSSRQFFNQLKLLPVQGDEVQLFKTLITIHKVLQEGHGSALKEAMRNADWIEGLGRSVHGDGFKGYGRLIKEYVVYLLRKLNFHKNHRGFNGTFEYEEYVSLVTVQNPDEGYEAILDLMTLQDALDDLQRLIFASISHDRKSECKISALVPLVAESYGIYKFITSMLRAIHRSTGSDEALEPLRERYESQHARLYEFYADCSSIRYLTSLITIPRLPETAPNLISKDESDVESERPASRQTSVATPVPEPISEQPTMGNFQQPIASQPTGVDFWNTQQNVFEAEQQRLAREAQENMLQQQQQAEQQRIWFEQQQAAQAEQQRLAQEQLLRDQLQNQAEGRVAELERDLLAMRQQYETDQLLL +-1_616124-616402 - 616124 616402 93 [] MATALPHA2 21 KDIERLHGLSETELTCFQDELGDIEVLGRKAKLLLDERQSLNDHIMDFHTSVKGADKAMIFDVLTQDIMCHRQTQGIKKKRLEKEKVEILEKW +-2_615961-616140 - 615961 616140 60 [('Homeobox_KN', 17.6)] MATALPHA2 19 RYLKSGKSVNIFFFSFPETADILILRCRYSHQNQKAYLKEDVLSMLVSETGLTKVQIRNW +-2_611410-612387 - 611410 612387 326 [] dic1 376 RARALSSPHHPNHITPHAMSSIASKTESTLSSSPSSTTSHKVDVTPRQKWPFWYGGFGGGVACLFTHPLDLAKVRLQTAPIPKPTLFQMAKSILVHDGLLGVYSGLSAGLLRQATYSLTRFGVYEYLKENVVPADKQSSMAYLLPVSMFSGGIGGVVGNPADIVNIRMQNDTGLAPDQRRNYKHAIDGVIRVAREEGTSALFRGLGTNLVRGVLMTSSQVVTYDVAKDWLISFGMNKDSISTFFSASLLSGLVATTICSPADVLKTRIMNSSGTGESVVHILTTAFKNEGPSFMFRGWVPSFVRLGPNTIITFLVVEQLKHLRVGM diff --git a/results/2026-10-30_petasospora/data/scan_alpha_tbrc4498_sens.tsv b/results/2026-10-30_petasospora/data/scan_alpha_tbrc4498_sens.tsv new file mode 100644 index 00000000..59001960 --- /dev/null +++ b/results/2026-10-30_petasospora/data/scan_alpha_tbrc4498_sens.tsv @@ -0,0 +1,5 @@ +frag strand start end aa pfam best_ref bits seq ++0_636268-636666 + 636268 636666 133 [] ONH67106.1 139 GRLFGFDGCTCTTVLVYQRSQGKKKKKILTDLPLFKYLYFFFLQSLLFDSLCLPMTHDVLSQYIKNHSLVSSFNGGMKVHDVIVEALSFIKEELCFATQYFNITQLILEASQFSLREAMKPLDVLSSMLESTR ++0_637471-638454 + 637471 638454 328 [('MATalpha_HMGbox', 36.3)] ONH67106.1 407 HDWCFEATVCIVSGQEISVVRRRMIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASVTIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPPSQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDRPIVTLASDPSLSRSYIDPLVYEISSGTYEKS +-0_636364-636642 - 636364 636642 93 [] MATALPHA2 21 KDIERLHGLSETELTCFQDELGDIEVLGRKAKLLLDERQSLNDHIMDFHTSVKGADKAMIFDVLTQDIMCHRQTQGIKKKRLEKEKVEILEKW +-2_636200-636370 - 636200 636370 57 [('Homeobox_KN', 17.7), ('Homeodomain', 12.5)] MATALPHA2 18 KVVSPSIFFFFSFPETADILILWCRYSHQNQKAYLKEDVLSMLVSETGLTKVQIRNW diff --git a/results/2026-10-30_petasospora/make_records.py b/results/2026-10-30_petasospora/make_records.py new file mode 100644 index 00000000..78bf23c1 --- /dev/null +++ b/results/2026-10-30_petasospora/make_records.py @@ -0,0 +1,82 @@ +"""Write the two draft MATpf proposals for Petasospora (Cyberlindnera) fabianii into this directory (NOT db/). + +MATa allele: JOY008 (St. Louis College of Pharmacy; ecotype Missouri), complete genome GCA_022641835.1, CP048715.1. +MATalpha allele: TBRC 4498, chromosome-level GCA_050575385.1, CM115473.1 (the strain-65 draft, GCA_001983305.1, carries the same locus and + the depositor's ONH67106 model; JCM 3601 GCA_001599195.1 carries the MATa locus again). +Gene models are our own: AUGUSTUS ab initio (no RNA-seq exists for the species) selected by agreement of several yeast species models and by +homology/profile evidence; coordinates include the stop codon. Run from the repo root: + python3 results/2026-10-30_petasospora/make_records.py +""" +import yaml + +HERE = "results/2026-10-30_petasospora/" +# (gene, role, strand, exons in genomic order, stop-codon-excluded as AUGUSTUS gives them) +A = ("CP048715.1", [("dic1", "flanking_conserved", "-", [(628452, 629375)]), + ("MATa2", "core_MAT", "-", [(629853, 630419), (630492, 630863)]), + ("MATa1", "core_MAT", "+", [(631437, 631737), (631835, 632133)]), + ("sla2", "flanking_conserved", "+", [(641746, 644868)])]) +AL = ("CM115473.1", [("dic1", "flanking_conserved", "-", [(631649, 632572)]), + ("MATalpha1", "core_MAT", "+", [(637540, 638454)]), + ("sla2", "flanking_conserved", "+", [(648035, 651157)])]) + +def gene(idx, name, role, strand, ex): + ex = sorted(ex) + if strand == "+": ex[-1] = (ex[-1][0], ex[-1][1] + 3) # add the stop codon + else: ex[0] = (ex[0][0] - 3, ex[0][1]) + tx = ex if strand == "+" else ex[::-1] + return {"gene_index": idx, "name": name, "protein_accession": None, "role": role, "present": True, "locus_tag": None, + "segment_index": 0, "start": min(a for a, b in ex), "end": max(b for a, b in ex), "strand": strand, "order_in_locus": idx, + "exons": [{"start": a, "end": b} for a, b in tx]} + +TAX = {"taxid": 36022, "lineage": "k__Fungi;p__Ascomycota;subphylum__Saccharomycotina;c__Saccharomycetes;o__Phaffomycetales;f__Wickerhamomycetaceae;g__Petasospora;s__Petasospora_fabianii", + "lineage_resolved_date": "2026-10-30"} +CIT_65 = [{"pmid": "28385833", "doi": "10.1128/genomeA.00064-17"}] +CIT_TBRC = [{"pmid": "40558965", "doi": "10.3390/jof11060453"}] +COMMON = ("GENE MODELS ARE OURS (tier 3, manual annotation; curator decision 2026-10-30). No RNA-seq exists for the species in ENA/SRA (checked 2026-10-30), " + "so the models are AUGUSTUS ab initio predictions (species models S. cerevisiae S288C, C. guilliermondii, P. stipitis, K. lactis) chosen where several " + "agree and where homology supports them; MATa2 is also supported by the Saccharomycotina MATa2 profile (76.8 bits, above the 52.4 bits of P. kudriavzevii). " + "Coordinates include the stop codon. Intron signals: MATa2 72 bp GTAGGT..TACTAAC..TAG (strong); MATa1 97 bp GTACCA..AAAG (weak donor; an upstream exon is required because the homeodomain open frame has no start codon, but the " + "splice sites are unconfirmed). The MATalpha1 model is a single exon of 305 aa; the depositor's ONH67106 (254 aa, strain 65) is the same C-terminal 185 aa plus a " + "69 aa exon joined across a 1,263 bp intron (GTCCTT..CCCCAG) that has no support here: the N-terminus is unresolved. GENE NAMES by homology and position: " + "MATa1 is the homeodomain protein (Pfam Homeodomain 64 bits, 66 bits to Komagataella MATa1), MATa2 the HMG-box protein (divergent; no Pfam HMG_box hit above GA), " + "MATalpha1 the alpha-box protein (PF04769 36 bits, 407 bits to ONH67106). No MATalpha2 homeodomain gene was found in either alpha allele (only a 57 aa Homeobox_KN fragment at " + "12-18 bits about 1.2 kb upstream of MATalpha1). LAYOUT: DIC1 (minus) - 0.5 kb (MATa) or 5.0 kb (MATalpha) - MAT genes - six conserved non-MAT ORFs (409, 173, 589, 219, 465, 622 aa; identical in both alleles, not recorded) - SLA2 (plus); " + "SLA2 is therefore about 9.5 kb from the MAT genes. The alpha allele also carries an 800 aa MutS_III-domain ORF between DIC1 and MATalpha1 that has no counterpart in the MATa allele (not recorded). " + "No second MAT cassette: miniprot of the MAT proteins finds no other MAT gene in any of the four assemblies, so the species looks heterothallic with one idiomorph per genome. " + "GENETIC CODE: standard (table 1). Details: results/2026-10-30_petasospora/README.md.") + +def record(rid, idiomorph, contig, genes, strain, acc, cit, note, defn): + lo = min(g["start"] for g in genes); hi = max(g["end"] for g in genes) + return {"record_id": rid, "record_version": 1, "taxonomy": TAX, + "organism": {"species": "Petasospora fabianii", "strain": {"name": strain[0], "known": True, "culture_collection_ids": strain[1], "differs_from_sequenced": False}}, + "mating_type": {"locus_name": "MATpf", "idiomorphs": [idiomorph], "system": "heterothallic"}, + "locus": {"coordinate_provenance": "curator_derived", "excluded_from_coordinate_benchmark": False, + "assembly_accession": acc, "assembly_accession_basis": "sequence_link", + "assembly_location": {"contig": contig, "start": lo, "end": hi, "identity": 100.0}, + "core": {"completeness": "complete", "reference_orientation": defn, + "definition_note": note + " " + COMMON, + "segments": [{"segment_index": 0, "sequence_source": {"type": "insdc_nucleotide", "accession": contig, "seq_region": contig}, + "start": lo, "end": hi, "contig_edge_distance": None, "sequence_checksum": None}]}, + "extended_flank": []}, + "genes": genes, + "evidence": {"locus_existence": {"tier": 3, "experimental_method": "manual annotation: homology (curated Saccharomycotina MAT proteins, Pfam, MATa2 profile) and AUGUSTUS ab initio models on the deposited assembly; no RNA-seq", "citations": cit}, + "boundaries": {"tier": 3, "experimental_method": "AUGUSTUS ab initio gene models checked against homology; DIC1 and SLA2 are the flanking anchors, MAT genes lie between them", "citations": cit}, + "idiomorph_assignment": {"tier": 3, "experimental_method": "gene content: MATa1 (homeodomain) with MATa2 (HMG-box) versus MATalpha1 (alpha box), at the same DIC1-SLA2 position in different strains", "citations": cit}}, + "validation": {"status": "needs_review", "rejection_reason": None, "accession_resolved": False, "accession_resolved_date": None, + "accession_resolved_version": None, "sequence_match": {"status": "pass", "per_gene": [], "notes": ""}, "taxonomy_current": None}, + "curation": {"proposed_by": "claude-code-curation-agent", "proposal_dedupe_key": f"manual|36022|MATpf|{idiomorph}", "reviewed_by": None, "reviewed_date": None}, + "model_provenance": None} + +ga = [gene(i, n, r, s, e) for i, (n, r, s, e) in enumerate(A[1])] +gl = [gene(i, n, r, s, e) for i, (n, r, s, e) in enumerate(AL[1])] +NOTE_A = ("MATa idiomorph of P. fabianii isolate JOY008 (ecotype Missouri, St. Louis College of Pharmacy), complete genome GCA_022641835.1, CP048715.1 (BioSample SAMN14051719). " + "The type strain JCM 3601 (GCA_001599195.1, BCGI01000001.1:641,150-657,563) carries the same MATa locus with 99-100% identical proteins (MATa1 and MATa2 one substitution each). " + "The v0.7.0 MATsc call (CP048715.1:630,345-685,843, 55 kb) is wider than the locus: the genes are DIC1 628,452-629,375 to SLA2 641,746-644,871.") +NOTE_AL = ("MATalpha idiomorph of P. fabianii TBRC 4498 (xylitol-producing strain; PMID 40558965), chromosome-level GCA_050575385.1, CM115473.1. The draft genome of strain 65 from fermented masau fruit " + "(GCA_001983305.1, MPUK01000005.1:611,410-630,920; PMID 28385833) carries the same locus with an identical MATalpha1 protein and holds the depositor's ONH67106 " + "('Mating-type-like protein ALPHA1', 254 aa, MPUK01000005.1:616,190-616,396 + 617,660-618,217), which our model replaces.") +recs = [record("36022_joy008_MATpf_MATa", "MATa", A[0], ga, ("JOY008", ["JOY008"]), "GCA_022641835.1", [], NOTE_A, "DIC1->MATa2->MATa1->(six conserved genes)->SLA2"), + record("36022_tbrc-4498_MATpf_MATalpha", "MATalpha", AL[0], gl, ("TBRC 4498", ["TBRC 4498"]), "GCA_050575385.1", CIT_TBRC + CIT_65, NOTE_AL, "DIC1->MATalpha1->(six conserved genes)->SLA2")] +for r in recs: + open(f"{HERE}{r['record_id']}.yaml", "w").write(yaml.safe_dump(r, sort_keys=False, width=120)) + print(r["record_id"], [(g["name"], g["start"], g["end"], g["strand"], len(g["exons"])) for g in r["genes"]]) diff --git a/results/2026-10-30_petasospora/make_windows.py b/results/2026-10-30_petasospora/make_windows.py new file mode 100644 index 00000000..6e95ece3 --- /dev/null +++ b/results/2026-10-30_petasospora/make_windows.py @@ -0,0 +1,11 @@ +"""Extract the four MAT windows (DIC1 start -2 kb to SLA2 end +1 kb) into data/windows.fa; header: name contig start end.""" +import subprocess, sys +D = sys.argv[1] # directory with the four .fa +W = [("GCA_022641835.1_ASM2264183v1", "CP048715.1", 626500, 645500, "pf_a_cp048715"), + ("GCA_001599195.1_JCM_3601_assembly_v001", "BCGI01000001.1", 639000, 658500, "pf_a_jcm3601"), + ("GCA_001983305.1_ASM198330v1", "MPUK01000005.1", 609500, 631500, "pf_alpha_strain65"), + ("GCA_050575385.1_ASM5057538v1", "CM115473.1", 629500, 652000, "pf_alpha_tbrc4498")] +with open("data/windows.fa", "w") as o: + for g, c, a, b, n in W: + s = "".join(subprocess.check_output(["samtools", "faidx", f"{D}/{g}.fa", f"{c}:{a}-{b}"], text=True).split("\n")[1:]) + o.write(f">{n} {c} {a} {b}\n" + "\n".join(s[i:i + 80] for i in range(0, len(s), 80)) + "\n") diff --git a/results/2026-10-30_petasospora/order_MATpf_proposal.yml b/results/2026-10-30_petasospora/order_MATpf_proposal.yml new file mode 100644 index 00000000..e6e5e9fe --- /dev/null +++ b/results/2026-10-30_petasospora/order_MATpf_proposal.yml @@ -0,0 +1,17 @@ +# PROPOSED family block for db/Ascomycota/order.yml (NOT applied; the curator decides name, scope and gene classes). +# Scope: species Petasospora fabianii (36022), as MATpk was narrowed to the species; the genus Petasospora would be wider than the evidence (one species). + - locus_name: "MATpf" + vocabulary_type: "enum" + idiomorph_values: ["MATa", "MATalpha"] + idiomorph_class: {MATalpha: unassigned, MATa: unassigned} + taxonomic_scope: [36022] + fallback_subphylum: 147537 # Saccharomycotina + fallback_searchable: false + flank_carried_window_bp: 20000 + genes: + - {name: MATa1, role: core_MAT, present_in_idiomorphs: ["MATa"]} # homeodomain (Pfam Homeodomain 64 bits) + - {name: MATa2, role: core_MAT, present_in_idiomorphs: ["MATa"]} # HMG-box; no Pfam hit above GA, MATa2 Saccharomycotina profile 76.8 bits + - {name: MATalpha1, role: core_MAT, gene_class: alpha_box, present_in_idiomorphs: ["MATalpha"]} # PF04769 36 bits + - {name: MATalpha2, role: core_MAT, present_in_idiomorphs: ["MATalpha"], optional: true} # none found in either alpha allele + - {name: dic1, role: flanking_conserved, optional: true} + - {name: sla2, role: flanking_conserved, gene_class: sla2_homolog, optional: true} diff --git a/results/2026-10-30_petasospora/pf_augustus.slurm b/results/2026-10-30_petasospora/pf_augustus.slurm new file mode 100644 index 00000000..d7524d5a --- /dev/null +++ b/results/2026-10-30_petasospora/pf_augustus.slurm @@ -0,0 +1,11 @@ +#!/bin/bash +#SBATCH -p exfab -A exfab -c 2 --mem 4G -t 00:30:00 -J pf-augustus +#SBATCH -o /bigdata/stajichlab/jstajich/projects/MATPredict/logs/pf_augustus_%j.log +# Ab initio AUGUSTUS predictions on the four P. fabianii MAT windows (no RNA-seq exists), three yeast species models. +set -euo pipefail +W=/bigdata/stajichlab/jstajich/projects/MATPredict/results/2026-10-30_petasospora/work; cd $W +set +eu; source /etc/profile 2>/dev/null; module load augustus/3.5.0; set -eu +for sp in saccharomyces_cerevisiae_S288C candida_guilliermondii pichia_stipitis kluyveromyces_lactis; do + augustus --species=$sp --genemodel=complete --strand=both --protein=on --codingseq=on --gff3=on windows.fa > aug_$sp.gff3 2> aug_$sp.err || echo "fail $sp" +done +touch ../DONE_AUG diff --git a/results/2026-10-30_petasospora/pf_miniprot.slurm b/results/2026-10-30_petasospora/pf_miniprot.slurm new file mode 100644 index 00000000..b5c77702 --- /dev/null +++ b/results/2026-10-30_petasospora/pf_miniprot.slurm @@ -0,0 +1,9 @@ +#!/bin/bash +#SBATCH -p exfab -A exfab -c 4 --mem 8G -t 01:00:00 -J pf-miniprot +#SBATCH -o /bigdata/stajichlab/jstajich/projects/MATPredict/logs/pf_miniprot_%j.log +# P. fabianii: curated Saccharomycotina MAT proteins + ONH67106 aligned with miniprot to four assemblies (no RNA-seq exists for the species). +set -euo pipefail +W=/bigdata/stajichlab/jstajich/projects/MATPredict/results/2026-10-30_petasospora/work; cd $W +export PATH=/bigdata/stajichlab/jstajich/projects/MATPredict/.pixi/envs/default/bin:$PATH +for f in GCA_*.fna.gz; do n=${f%.fna.gz}; zcat $f > $n.fa; miniprot -t4 -d $n.mpi $n.fa 2>/dev/null; miniprot -t4 --gff --outs=0.5 -P PF $n.mpi refs.faa > $n.miniprot.gff 2> $n.miniprot.log; done +touch ../DONE diff --git a/results/2026-10-30_petasospora/scan_window.py b/results/2026-10-30_petasospora/scan_window.py new file mode 100644 index 00000000..089cc583 --- /dev/null +++ b/results/2026-10-30_petasospora/scan_window.py @@ -0,0 +1,47 @@ +"""Six-frame stop-to-stop fragments (>= MINLEN aa) of a genomic window, scanned with Pfam-A (MAT-relevant domains) and +phmmer against the MAT reference proteins. Usage: scan_window.py FASTA CONTIG START END OUT.tsv [MINLEN]""" +import sys, subprocess, pyhmmer +from pyhmmer.easel import SequenceFile, Alphabet, TextSequence +fa, contig, a, b, out = sys.argv[1], sys.argv[2], int(sys.argv[3]), int(sys.argv[4]), sys.argv[5] +MINLEN = int(sys.argv[6]) if len(sys.argv) > 6 else 40 +seq = "".join(subprocess.check_output(["samtools", "faidx", fa, f"{contig}:{a}-{b}"], text=True).split("\n")[1:]) +CODON = {} +bases = "TCAG"; aas = "FFLLSSSSYY**CC*WLLLLPPPPHHQQRRRRIIIMTTTTNNKKSSRRVVVVAAAADDEEGGGG" +for i, x in enumerate(bases): + for j, y in enumerate(bases): + for k, z in enumerate(bases): CODON[x + y + z] = aas[16 * i + 4 * j + k] +def rc(s): return s[::-1].translate(str.maketrans("ACGTacgtN", "TGCAtgcaN")) +def tr(s): return "".join(CODON.get(s[i:i + 3].upper(), "X") for i in range(0, len(s) - 2, 3)) +frags = [] +for strand, s in (("+", seq), ("-", rc(seq))): + for f in range(3): + p = tr(s[f:]); pos = 0 + for piece in p.split("*"): + if len(piece) >= MINLEN: + st = f + 3 * pos; en = st + 3 * len(piece) # nt offsets in s + if strand == "+": g0, g1 = a + st, a + en - 1 + else: g0, g1 = b - en + 1, b - st + frags.append((strand, f, g0, g1, piece)) + pos += len(piece) + 1 +def _s(x): return x.decode() if isinstance(x, bytes) else x +alpha = Alphabet.amino() +ts = [TextSequence(name=f"{s}{f}_{g0}-{g1}".encode(), sequence=p).digitize(alpha) for s, f, g0, g1, p in frags] +dom = {} +want = {"HMG_box", "HMG_box_2", "Homeodomain", "Homeobox_KN", "MATalpha_HMGbox", "I_LWEQ", "ANTH", "ENTH"} +with pyhmmer.plan7.HMMFile("/Users/jstajich/db/funannotate/Pfam-A.hmm") as hf: + for hits in pyhmmer.hmmscan(ts, hf, E=1e-2): + q = _s(hits.query.name) + dom[q] = [(_s(h.name), round(h.score, 1)) for h in hits if _s(h.name) in want] +with SequenceFile(sys.argv[0].rsplit("/", 1)[0] + "/../2026-10-29_ss_records/refs.faa" if False else "/Users/jstajich/projects/MATPredict/results/2026-10-30_petasospora/data/all_refs.faa", digital=True, alphabet=alpha) as sf: db = sf.read_block() +best = {} +for hits in pyhmmer.phmmer(ts, db, E=1e-3): + if len(hits): + h = hits[0]; best[_s(hits.query.name)] = (_s(h.name), h.score) +with open(out, "w") as o: + o.write("frag\tstrand\tstart\tend\taa\tpfam\tbest_ref\tbits\tseq\n") + for (s_, f, g0, g1, p), t in zip(frags, ts): + n = _s(t.name); d = dom.get(n, []); br = best.get(n) + if d or br: + nm = br[0] if br else "" + nm = (nm.split("name=")[1].split("|")[0] if "name=" in nm else nm)[-45:] + o.write(f"{n}\t{s_}\t{g0}\t{g1}\t{len(p)}\t{d}\t{nm}\t{br[1]:.0f}\t{p}\n" if br else f"{n}\t{s_}\t{g0}\t{g1}\t{len(p)}\t{d}\t\t\t{p}\n") diff --git a/results/2026-10-30_petasospora/summarize_miniprot.py b/results/2026-10-30_petasospora/summarize_miniprot.py new file mode 100644 index 00000000..c3c65d39 --- /dev/null +++ b/results/2026-10-30_petasospora/summarize_miniprot.py @@ -0,0 +1,18 @@ +"""Per-genome table of miniprot hits of MAT-related reference proteins: best hit per gene class and its coordinates.""" +import re, sys, glob, collections +for fn in sorted(glob.glob("data/*.miniprot.gff")): + rows = [] + for l in open(fn): + f = l.rstrip("\n").split("\t") + if len(f) > 8 and f[2] == "mRNA": + t = re.search(r"Target=(\S+)", f[8]).group(1) + idn = float(re.search(r"Identity=([\d.]+)", f[8]).group(1)); pos = float(re.search(r"Positive=([\d.]+)", f[8]).group(1)) + m = re.search(r"name=([^|\s;]+)", t); gene = m.group(1) if m else t.split("|")[-1].split("_")[-1] + rows.append((f[0], int(f[3]), int(f[4]), f[6], gene, idn, t)) + print("==", fn.split("/")[-1][:30]) + by = collections.defaultdict(list) + for r in rows: by[(r[0], r[4])].append(r) + for (c, g), rs in sorted(by.items(), key=lambda kv: (kv[0][0], min(x[1] for x in kv[1]))): + b = max(rs, key=lambda x: x[5]) + if b[5] >= 0.3 or g in ("ONH67106", "XP_020071302"): + print(f" {c}\t{b[1]}-{b[2]}\t{b[3]}\t{g}\tid={b[5]:.2f}\tn={len(rs)}") diff --git a/results/2026-10-30_saccharomycopsis/README.md b/results/2026-10-30_saccharomycopsis/README.md index ebc57009..3aa75bd5 100644 --- a/results/2026-10-30_saccharomycopsis/README.md +++ b/results/2026-10-30_saccharomycopsis/README.md @@ -13,3 +13,6 @@ Curator decisions (2026-10-30): draft records from our own gene models (tier 3); - Both SLA2 ORFs lack a start codon (5-prime partial). The mirrored arm of the palindrome is not recorded. - The label registry has no entry for `Ascomycota:MATss`; a system code needs your approval before the records are labelled (frozen codes). - Scope is the genus; *S. lassenensis* is heterothallic (Dost et al.), so narrow to the species (44080) if a replay over-calls. + +## The other two genera (same session) +`MATpf` (*Petasospora fabianii*, species scope 36022; heterothallic, MATa JOY008 and MATalpha TBRC 4498; AUGUSTUS and miniprot models, no RNA-seq exists) and `MATbz` (*Barnettozyma*, genus scope 599802; one combined PB4207 record, MATalpha2 not modelled) were annotated in `../2026-10-30_petasospora/` and `../2026-10-30_barnettozyma/`. All five candidate records pass `curate-db propose` and `validate` (Slurm job 31446171: `taxonomy_current` true, sequence match not applicable because no record has a protein accession). All are tier 3 (our own models), `needs_review`. diff --git a/results/2026-10-30_saccharomycopsis/validate.slurm b/results/2026-10-30_saccharomycopsis/validate.slurm index 14576c28..61d958f8 100644 --- a/results/2026-10-30_saccharomycopsis/validate.slurm +++ b/results/2026-10-30_saccharomycopsis/validate.slurm @@ -5,7 +5,7 @@ R=/bigdata/stajichlab/jstajich/projects/MATPredict T=$R/results/2026-10-30_saccharomycopsis/tree export PATH="$R/.pixi/envs/default/bin:$PATH" cd $T -for r in 44080_gdd12_MATss_MATalpha 44080_gdd12_MATss_MATa; do +for r in 44080_gdd12_MATss_MATalpha 44080_gdd12_MATss_MATa 36022_joy008_MATpf_MATa 36022_tbrc-4498_MATpf_MATalpha 36038_pb4207_MATbz_MATa-MATalpha; do PYTHONPATH="$T/src" MATPREDICT_DB_ROOT="$T/db" python -m MATPredict curate-db validate --phylum Ascomycota --record-id $r > $T/$r.validate.json grep -n "taxonomy_current\|taxonomy_error\|sequence_match\|status" -A1 $T/$r.validate.json | head -12 done diff --git a/tests/detect/test_fallback_searchable.py b/tests/detect/test_fallback_searchable.py index 7f9e3558..0b209990 100644 --- a/tests/detect/test_fallback_searchable.py +++ b/tests/detect/test_fallback_searchable.py @@ -53,7 +53,7 @@ def test_shipped_roster_flags_the_scope_only_families(): Yarrowia `MATyl` and Geotrichum `MATdc` (curator 2026-10-17), Komagataella `MATkp` and the Ogataea polymorpha-complex `MATog` (curator 2026-10-17/18).""" flagged = {f.key.locus_name for f in load_all_families(DB) if not f.fallback_searchable} - assert flagged == {"redPR", "redHD", "rustHD", "wallMAT", "MATyl", "MATdc", "MATkp", "MATog", "MATpk", "MATss"} + assert flagged == {"redPR", "redHD", "rustHD", "wallMAT", "MATyl", "MATdc", "MATkp", "MATog", "MATpk", "MATss", "MATpf", "MATbz"} def test_pr_scope_includes_boletales(): From 21b145cbefaf606843a506cea0cb290cb40470bc Mon Sep 17 00:00:00 2001 From: Jason Stajich Date: Fri, 30 Oct 2026 06:25:13 -0700 Subject: [PATCH 3/5] MATss scope narrowed to Saccharomycopsis schoenii (44080); heterothallic expectation for other species documented Co-Authored-By: Claude Sonnet 5.5 --- db/Ascomycota/order.yml | 12 +++++++----- results/2026-10-30_saccharomycopsis/README.md | 4 ++-- tests/detect/test_matss_family.py | 9 ++++++--- 3 files changed, 15 insertions(+), 10 deletions(-) diff --git a/db/Ascomycota/order.yml b/db/Ascomycota/order.yml index 2a93012c..4413901f 100644 --- a/db/Ascomycota/order.yml +++ b/db/Ascomycota/order.yml @@ -808,11 +808,13 @@ loci: # `two_idiomorphs_report` stays off (a homothallic species carries both idiomorphs by design). idiomorph_values: ["MATa", "MATalpha"] idiomorph_class: {MATalpha: unassigned, MATa: unassigned} - # SCOPE = THE GENUS Saccharomycopsis (taxid 4943; NCBI Taxonomy efetch 2026-10-30: rank genus, family Saccharomycopsidaceae 34366, - # order Ascoideales 2926619). Only S. schoenii has a record. Dost et al. 2024 describe S. lassenensis as heterothallic, so the genus is - # not uniform; the scope is the genus because 22 of the 27 loci the replay found in the genus have the SLA2-inside architecture, - # and it is the curator's to narrow to the species (44080) if a replay over the six Saccharomycopsis species over-calls. - taxonomic_scope: [4943] + # SCOPE = THE SPECIES Saccharomycopsis schoenii (taxid 44080; genus Saccharomycopsis 4943, family Saccharomycopsidaceae 34366, order + # Ascoideales 2926619; NCBI Taxonomy efetch 2026-10-30). Curator decision 2026-10-30: narrowed from the genus. Only S. schoenii has a record + # and it is homothallic with an expanded SLA2-MAT cassette system; the genus is NOT uniform: S. lassenensis is heterothallic (Dost et al. 2024, + # PMID 38492364), so a heterothallic species of the genus is EXPECTED to carry a single locus with one idiomorph per genome, a different + # architecture from the one recorded here. Other Saccharomycopsis genomes stay in phylum fallback (and, as before, can still be called by + # MATsc or MTL until a record of a second species exists). + taxonomic_scope: [44080] fallback_subphylum: 147537 # Saccharomycotina fallback_searchable: false flank_carried_window_bp: 20000 diff --git a/results/2026-10-30_saccharomycopsis/README.md b/results/2026-10-30_saccharomycopsis/README.md index 3aa75bd5..f46baf6c 100644 --- a/results/2026-10-30_saccharomycopsis/README.md +++ b/results/2026-10-30_saccharomycopsis/README.md @@ -2,7 +2,7 @@ Curator decisions (2026-10-30): draft records from our own gene models (tier 3); a scope-only family per genus, as for MATkp, MATog and MATpk. -- **Family** `MATss` (scope: genus *Saccharomycopsis*, taxid 4943; scope-only, never searched on a phylum fallback) in `db/Ascomycota/order.yml`. +- **Family** `MATss` (scope: the species *S. schoenii*, taxid 44080, narrowed from the genus on 2026-10-30 because *S. lassenensis* is heterothallic, so other species are expected to differ; scope-only, never searched on a phylum fallback) in `db/Ascomycota/order.yml`. - **Records** (candidates, `needs_review`): `44080_gdd12_MATss_MATalpha` (left sub-cluster: MATalpha1, MATalpha2, sla2) and `44080_gdd12_MATss_MATa` (right palindromic cassette: sla2, MATa1). Homothallic. Strain GDD12, assembly GCA_054953955.1, chromosome IV CM143129.1. - **Evidence:** Kriti et al., G3 2026 ([10.1093/g3journal/jkag067](https://doi.org/10.1093/g3journal/jkag067), PMID 41849659) for the SLA2-MAT architecture and locus spans; Dost et al., Microbiol Res 2024 ([10.1016/j.micres.2024.127691](https://doi.org/10.1016/j.micres.2024.127691), PMID 38492364) for homothallism. **Gene coordinates are ours**: the authors' GTF is not public. Models, method and limits: `../2026-10-29_ss_records/README.md`. - **Genetic code:** NCBI table 12 (CUG = Ser); every gene carries `transl_table: 12`. @@ -12,7 +12,7 @@ Curator decisions (2026-10-30): draft records from our own gene models (tier 3); - Gene naming: both homeodomain ORFs are closest to MATa1-type references (phmmer 50 and 47 bits to *C. albicans* MTLA1); the left one is named MATalpha2 after the paper by position only. An HMG-like ORF (470,349-470,951) is not assigned. No MATa2 in the right cassette. - Both SLA2 ORFs lack a start codon (5-prime partial). The mirrored arm of the palindrome is not recorded. - The label registry has no entry for `Ascomycota:MATss`; a system code needs your approval before the records are labelled (frozen codes). -- Scope is the genus; *S. lassenensis* is heterothallic (Dost et al.), so narrow to the species (44080) if a replay over-calls. +- Scope is the species (44080); a heterothallic *Saccharomycopsis* species (*S. lassenensis*) is expected to have one locus with one idiomorph per genome. Other species stay in phylum fallback. ## The other two genera (same session) `MATpf` (*Petasospora fabianii*, species scope 36022; heterothallic, MATa JOY008 and MATalpha TBRC 4498; AUGUSTUS and miniprot models, no RNA-seq exists) and `MATbz` (*Barnettozyma*, genus scope 599802; one combined PB4207 record, MATalpha2 not modelled) were annotated in `../2026-10-30_petasospora/` and `../2026-10-30_barnettozyma/`. All five candidate records pass `curate-db propose` and `validate` (Slurm job 31446171: `taxonomy_current` true, sequence match not applicable because no record has a protein accession). All are tier 3 (our own models), `needs_review`. diff --git a/tests/detect/test_matss_family.py b/tests/detect/test_matss_family.py index add3136f..c0eda898 100644 --- a/tests/detect/test_matss_family.py +++ b/tests/detect/test_matss_family.py @@ -1,4 +1,4 @@ -"""The MATss (Saccharomycopsis) family and its two S. schoenii candidate records (curator, 2026-10-30).""" +"""The MATss (Saccharomycopsis schoenii) family and its two S. schoenii candidate records (curator, 2026-10-30).""" from pathlib import Path import yaml @@ -14,7 +14,7 @@ def _family(): def test_matss_declaration(): fam = _family() - assert list(fam.taxonomic_scope) == [4943] + assert list(fam.taxonomic_scope) == [44080] assert fam.fallback_searchable is False and fam.cassette_report is False and fam.two_idiomorphs_report is False genes = {g["name"]: g for g in fam.genes} assert genes["MATalpha1"]["gene_class"] == "alpha_box" @@ -31,11 +31,14 @@ def test_matss_candidates_are_tier_3_models_with_table_12(): assert rec["validation"]["status"] == "needs_review" -def test_matss_routes_the_genus_only(): +def test_matss_routes_the_species_only(): fams = load_all_families(DB) base = [131567, 2759, 33154, 4751, 451864, 4890, 716545, 147537, 4891, 2926619, 34366, 4943] d = route(44080, fams, lineage_taxids_resolver=lambda t: base + [44080], phylum_name_resolver=lambda t: "Ascomycota") assert d.routing_mode == "lineage" and "MATss" in {f.key.locus_name for f in d.families} + # another Saccharomycopsis species (S. fibuligera 4944, not in scope) is not routed to MATss + d = route(4944, fams, lineage_taxids_resolver=lambda t: base + [4944], phylum_name_resolver=lambda t: "Ascomycota") + assert "MATss" not in {f.key.locus_name for f in d.families} d = route(4909, fams, lineage_taxids_resolver=lambda t: [131567, 2759, 33154, 4751, 451864, 4890, 716545, 147537, 4909], phylum_name_resolver=lambda t: "Ascomycota") assert "MATss" not in {f.key.locus_name for f in d.families} From 689b56c535afe4645d9765049efb5a62e485467a Mon Sep 17 00:00:00 2001 From: Jason Stajich Date: Fri, 30 Oct 2026 06:25:27 -0700 Subject: [PATCH 4/5] Fix MATss routing test (scope taxid routes direct) Co-Authored-By: Claude Sonnet 5.5 --- tests/detect/test_matss_family.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/detect/test_matss_family.py b/tests/detect/test_matss_family.py index c0eda898..a4216a83 100644 --- a/tests/detect/test_matss_family.py +++ b/tests/detect/test_matss_family.py @@ -35,7 +35,7 @@ def test_matss_routes_the_species_only(): fams = load_all_families(DB) base = [131567, 2759, 33154, 4751, 451864, 4890, 716545, 147537, 4891, 2926619, 34366, 4943] d = route(44080, fams, lineage_taxids_resolver=lambda t: base + [44080], phylum_name_resolver=lambda t: "Ascomycota") - assert d.routing_mode == "lineage" and "MATss" in {f.key.locus_name for f in d.families} + assert d.routing_mode in ("direct", "lineage") and "MATss" in {f.key.locus_name for f in d.families} # another Saccharomycopsis species (S. fibuligera 4944, not in scope) is not routed to MATss d = route(4944, fams, lineage_taxids_resolver=lambda t: base + [4944], phylum_name_resolver=lambda t: "Ascomycota") assert "MATss" not in {f.key.locus_name for f in d.families} From dfdc86ab0954f8aa7a8a20789e9d592ba5b62aa8 Mon Sep 17 00:00:00 2001 From: Jason Stajich Date: Fri, 30 Oct 2026 07:27:00 -0700 Subject: [PATCH 5/5] Profiles for MATa1/MATalpha2/MATa2 (results/2026-10-30_mat_hmms); S. schoenii records rebuilt with corrected gene names (HMG-like ORF is MATalpha2, left homeodomain MATa1, MATa2 added in the right cassette); B. californica gets a partial MATalpha2 Co-Authored-By: Claude Sonnet 5.5 --- db/Ascomycota/order.yml | 10 +- .../metadata.yaml | 41 +- .../metadata.yaml | 66 +- .../44080_gdd12_MATss_MATa/metadata.yaml | 45 +- .../36038_pb4207_MATbz_MATa-MATalpha.yaml | 38 +- .../2026-10-30_barnettozyma/make_records.py | 13 +- results/2026-10-30_mat_hmms/.gitignore | 3 + results/2026-10-30_mat_hmms/README.md | 46 + results/2026-10-30_mat_hmms/a2_loo.py | 9 + results/2026-10-30_mat_hmms/a2_loo.slurm | 8 + results/2026-10-30_mat_hmms/a2_orf.py | 16 + results/2026-10-30_mat_hmms/ablation.py | 18 + results/2026-10-30_mat_hmms/ablation.slurm | 9 + results/2026-10-30_mat_hmms/bc_alpha2_scan.py | 57 + results/2026-10-30_mat_hmms/build_sets.py | 49 + .../2026-10-30_mat_hmms/bz_windows_scan.py | 17 + results/2026-10-30_mat_hmms/data/ablation.tsv | 57 + .../data/bz_windows_scan.tsv | 26 + .../data/curated_mat_proteins.faa | 86 ++ results/2026-10-30_mat_hmms/data/loco.tsv | 109 ++ .../2026-10-30_mat_hmms/data/pfam_hits.tsv | 23 + .../2026-10-30_mat_hmms/data/public_a1.faa | 343 ++++++ .../2026-10-30_mat_hmms/data/public_a2.faa | 265 +++++ .../data/public_alpha2.faa | 996 ++++++++++++++++++ .../2026-10-30_mat_hmms/data/public_ids.json | 1 + results/2026-10-30_mat_hmms/data/set_a1.faa | 58 + results/2026-10-30_mat_hmms/data/set_a2.faa | 28 + .../2026-10-30_mat_hmms/data/set_alpha1.faa | 24 + .../2026-10-30_mat_hmms/data/set_alpha2.faa | 50 + results/2026-10-30_mat_hmms/data/set_meta.tsv | 81 ++ .../data/ss_genome_scan.tsv | 30 + .../data/test_proteins.faa | 46 + .../2026-10-30_mat_hmms/data/test_rank.tsv | 24 + .../2026-10-30_mat_hmms/data/test_scores.tsv | 26 + results/2026-10-30_mat_hmms/fetch_public.py | 27 + results/2026-10-30_mat_hmms/fetch_public2.py | 21 + results/2026-10-30_mat_hmms/hmm/a1.hmm | 554 ++++++++++ results/2026-10-30_mat_hmms/hmm/a2.hmm | 668 ++++++++++++ .../hmm/a2_no_Pichiaceae.hmm | 671 ++++++++++++ .../hmm/a2_no_Saccharomycetaceae.hmm | 641 +++++++++++ results/2026-10-30_mat_hmms/hmm/alpha1.hmm | 584 ++++++++++ results/2026-10-30_mat_hmms/hmm/alpha2.hmm | 653 ++++++++++++ results/2026-10-30_mat_hmms/inventory.py | 30 + results/2026-10-30_mat_hmms/loco.py | 62 ++ results/2026-10-30_mat_hmms/loco.slurm | 9 + results/2026-10-30_mat_hmms/make_tests.py | 39 + results/2026-10-30_mat_hmms/pfam_scan.py | 21 + results/2026-10-30_mat_hmms/score_models.py | 31 + results/2026-10-30_mat_hmms/score_tests.py | 46 + results/2026-10-30_mat_hmms/ss_a2_scan.py | 40 + results/2026-10-30_saccharomycopsis/README.md | 12 +- .../make_records.py | 36 +- .../validate.slurm | 2 +- tests/detect/test_matss_family.py | 2 +- 54 files changed, 6770 insertions(+), 97 deletions(-) rename db/candidates/Ascomycota/{44080_gdd12_MATss_MATalpha => 44080_gdd12_MATss_MATa-MATalpha}/metadata.yaml (67%) create mode 100644 results/2026-10-30_mat_hmms/.gitignore create mode 100644 results/2026-10-30_mat_hmms/README.md create mode 100644 results/2026-10-30_mat_hmms/a2_loo.py create mode 100644 results/2026-10-30_mat_hmms/a2_loo.slurm create mode 100644 results/2026-10-30_mat_hmms/a2_orf.py create mode 100644 results/2026-10-30_mat_hmms/ablation.py create mode 100644 results/2026-10-30_mat_hmms/ablation.slurm create mode 100644 results/2026-10-30_mat_hmms/bc_alpha2_scan.py create mode 100644 results/2026-10-30_mat_hmms/build_sets.py create mode 100644 results/2026-10-30_mat_hmms/bz_windows_scan.py create mode 100644 results/2026-10-30_mat_hmms/data/ablation.tsv create mode 100644 results/2026-10-30_mat_hmms/data/bz_windows_scan.tsv create mode 100644 results/2026-10-30_mat_hmms/data/curated_mat_proteins.faa create mode 100644 results/2026-10-30_mat_hmms/data/loco.tsv create mode 100644 results/2026-10-30_mat_hmms/data/pfam_hits.tsv create mode 100644 results/2026-10-30_mat_hmms/data/public_a1.faa create mode 100644 results/2026-10-30_mat_hmms/data/public_a2.faa create mode 100644 results/2026-10-30_mat_hmms/data/public_alpha2.faa create mode 100644 results/2026-10-30_mat_hmms/data/public_ids.json create mode 100644 results/2026-10-30_mat_hmms/data/set_a1.faa create mode 100644 results/2026-10-30_mat_hmms/data/set_a2.faa create mode 100644 results/2026-10-30_mat_hmms/data/set_alpha1.faa create mode 100644 results/2026-10-30_mat_hmms/data/set_alpha2.faa create mode 100644 results/2026-10-30_mat_hmms/data/set_meta.tsv create mode 100644 results/2026-10-30_mat_hmms/data/ss_genome_scan.tsv create mode 100644 results/2026-10-30_mat_hmms/data/test_proteins.faa create mode 100644 results/2026-10-30_mat_hmms/data/test_rank.tsv create mode 100644 results/2026-10-30_mat_hmms/data/test_scores.tsv create mode 100644 results/2026-10-30_mat_hmms/fetch_public.py create mode 100644 results/2026-10-30_mat_hmms/fetch_public2.py create mode 100644 results/2026-10-30_mat_hmms/hmm/a1.hmm create mode 100644 results/2026-10-30_mat_hmms/hmm/a2.hmm create mode 100644 results/2026-10-30_mat_hmms/hmm/a2_no_Pichiaceae.hmm create mode 100644 results/2026-10-30_mat_hmms/hmm/a2_no_Saccharomycetaceae.hmm create mode 100644 results/2026-10-30_mat_hmms/hmm/alpha1.hmm create mode 100644 results/2026-10-30_mat_hmms/hmm/alpha2.hmm create mode 100644 results/2026-10-30_mat_hmms/inventory.py create mode 100644 results/2026-10-30_mat_hmms/loco.py create mode 100644 results/2026-10-30_mat_hmms/loco.slurm create mode 100644 results/2026-10-30_mat_hmms/make_tests.py create mode 100644 results/2026-10-30_mat_hmms/pfam_scan.py create mode 100644 results/2026-10-30_mat_hmms/score_models.py create mode 100644 results/2026-10-30_mat_hmms/score_tests.py create mode 100644 results/2026-10-30_mat_hmms/ss_a2_scan.py diff --git a/db/Ascomycota/order.yml b/db/Ascomycota/order.yml index 4413901f..658cab26 100644 --- a/db/Ascomycota/order.yml +++ b/db/Ascomycota/order.yml @@ -820,10 +820,10 @@ loci: flank_carried_window_bp: 20000 genes: # Gene models of the records are OUR OWN (tier 3): the authors' GTF is not public (results/2026-10-29_ss_records/README.md). - # MATa1, MATalpha2: homeodomain; no class (the schema has none). Named after the paper's cassette (a: MATa1; alpha: MATalpha2); phmmer - # ranks both ORFs closest to MATa1-type references, so the name of the left-cluster homeodomain gene is a hypothesis. + # Gene NAMES rest on the MATa1, MATalpha2 and MATa2 profiles of results/2026-10-30_mat_hmms (supporting evidence, a score + # difference, not referenced by any rule). MATa1 and MATalpha2 are homeodomain proteins: no class (the schema has none). - {name: MATa1, role: core_MAT, present_in_idiomorphs: ["MATa"]} - # MATa2: optional; no HMG-box ORF was resolved in the right cassette (an HMG-like ORF 470,349-470,951 is not assigned). + # MATa2: optional (HMG-box, divergent; no Pfam HMG_box hit above GA, a2 profile 78 bits on the chromosome IV ORF). - {name: MATa2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATa"]} # MATalpha1: alpha_box (Pfam PF04769 54 bits, GA 28.1, on the chromosome IV ORF), the same bar as MATkp, MATog and MATpk. - {name: MATalpha1, role: core_MAT, gene_class: alpha_box, present_in_idiomorphs: ["MATalpha"]} @@ -852,7 +852,7 @@ loci: - {name: sla2, role: flanking_conserved, gene_class: sla2_homolog, optional: true} # NEW FAMILY, curator decision 2026-10-30: Barnettozyma (Phaffomycetales) has its own scope-only family, named after the genus; scope = the genus # (taxid 599802). One record, B. californica PB4207, from OUR OWN tier-3 models (no RNA-seq for the genus; the paper, Hession et al. 2025, - # PMID 41359681, gives no coordinates); see results/2026-10-30_barnettozyma/README.md. MATalpha2 is not modelled, hence optional. + # PMID 41359681, gives no coordinates); see results/2026-10-30_barnettozyma/README.md. MATalpha2 is a partial fragment (113 aa), hence optional. - locus_name: "MATbz" vocabulary_type: "enum" idiomorph_values: ["MATa", "MATalpha"] @@ -865,6 +865,6 @@ loci: - {name: MATa1, role: core_MAT, present_in_idiomorphs: ["MATa"]} - {name: MATa2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATa"]} # divergent HMG-like; no Pfam HMG_box hit - {name: MATalpha1, role: core_MAT, gene_class: alpha_box, present_in_idiomorphs: ["MATalpha"]} - - {name: MATalpha2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATalpha"]} # not modelled in PB4207 + - {name: MATalpha2, role: core_MAT, optional: true, present_in_idiomorphs: ["MATalpha"]} # partial fragment in PB4207 - {name: sla2, role: flanking_conserved, gene_class: sla2_homolog, optional: true} - {name: dic1, role: flanking_conserved, optional: true} diff --git a/db/candidates/Ascomycota/36038_pb4207_MATbz_MATa-MATalpha/metadata.yaml b/db/candidates/Ascomycota/36038_pb4207_MATbz_MATa-MATalpha/metadata.yaml index aead7ce1..9257cd65 100644 --- a/db/candidates/Ascomycota/36038_pb4207_MATbz_MATa-MATalpha/metadata.yaml +++ b/db/candidates/Ascomycota/36038_pb4207_MATbz_MATa-MATalpha/metadata.yaml @@ -30,7 +30,7 @@ locus: identity: 100.0 core: completeness: partial - reference_orientation: DIC1->MATa2->MATa1->MATalpha1->SLA2 (MATalpha2 not modelled) + reference_orientation: DIC1->MATa2->MATa1->MATalpha1->MATalpha2 (partial)->SLA2 definition_note: 'DRAFT, GENE MODELS ARE OURS (tier 3). B. californica strain PB4207 (Dalian Medical University, GCA_023627795.1, ctg_5 = JAMAIE010000005.1, 694,392 bp; the locus is 63 kb from one contig end). The one published description @@ -54,16 +54,18 @@ locus: score the same), MATa2 (269 aa, one 56 bp GTATGT..CTTAG intron at 614,112-614,167, 82 bits to the MATa2 profile against a background maximum of 32 over all 124 six-frame segments of the window; no Pfam HMG_box hit, as for Komagataella). - MATalpha2 IS NOT RECORDED: a homeodomain region (genome 620,370-620,700) follows - MATalpha1 but no complete gene model could be built; it is not recorded as present - or absent. NAMING: Pfam cannot tell the MATa1 homeodomain from the MATalpha2 - homeodomain; the names follow position (the homeodomain gene next to the HMG-like - gene on the MATa side is MATa1). The other three californica assemblies (UCD09 - GCA_003123585.1, UCD89 GCA_003123885.1, NRRL Y-17395 GCA_003706655.3) carry - the same proteins at 99.6-100% identity, which is one strain family, not independent - evidence. B. discipulorum (GCA_049863505.1) has SLA2, MATa1, MATalpha1 and DIC1 - at 45-86% identity but arranged differently and no MATa2 hit. GENETIC CODE: - standard (table 1), not tested. Details: results/2026-10-30_barnettozyma/README.md.' + MATalpha2 IS RECORDED AS PARTIAL (added 2026-10-30): a 113 aa alpha2-type homeodomain + ORF at 620,080-620,421 (plus strand; the alpha2 profile of results/2026-10-30_mat_hmms + scores 37 bits), the same ORF in all four californica assemblies; it begins + right after an in-frame stop, so the N-terminal exon is not found and the model + is a fragment. NAMING: the a1 and alpha2 profiles of results/2026-10-30_mat_hmms + (supporting evidence, a score difference) call the MATa1 homeodomain a1 (104 + bits against 23 for alpha2). The other three californica assemblies (UCD09 GCA_003123585.1, + UCD89 GCA_003123885.1, NRRL Y-17395 GCA_003706655.3) carry the same proteins + at 99.6-100% identity, which is one strain family, not independent evidence. + B. discipulorum (GCA_049863505.1) has SLA2, MATa1, MATalpha1 and DIC1 at 45-86% + identity but arranged differently and no MATa2 hit. GENETIC CODE: standard (table + 1), not tested. Details: results/2026-10-30_barnettozyma/README.md.' segments: - segment_index: 0 sequence_source: @@ -128,6 +130,18 @@ genes: end: 619103 gene_class: alpha_box - gene_index: 4 + name: MATalpha2 + role: core_MAT + present: true + segment_index: 0 + start: 620080 + end: 620421 + strand: + + order_in_locus: 4 + exons: + - start: 620080 + end: 620421 +- gene_index: 5 name: sla2 role: flanking_conserved present: true @@ -135,7 +149,7 @@ genes: start: 627731 end: 630871 strand: '-' - order_in_locus: 4 + order_in_locus: 5 exons: - start: 627731 end: 630871 @@ -156,7 +170,8 @@ evidence: tier: 3 experimental_method: 'gene content and position: HMG-like MATa2 + homeodomain MATa1 on one side, alpha-box MATalpha1 on the other, separated by 2.8 kb (PMID - 41359681); homeodomain names by position only' + 41359681); the a1 profile of results/2026-10-30_mat_hmms calls the MATa1 homeodomain + a1 (104 vs 23 bits); MATa2 scores 81-82 bits on the a2 profiles' citations: *id001 validation: status: needs_review diff --git a/db/candidates/Ascomycota/44080_gdd12_MATss_MATalpha/metadata.yaml b/db/candidates/Ascomycota/44080_gdd12_MATss_MATa-MATalpha/metadata.yaml similarity index 67% rename from db/candidates/Ascomycota/44080_gdd12_MATss_MATalpha/metadata.yaml rename to db/candidates/Ascomycota/44080_gdd12_MATss_MATa-MATalpha/metadata.yaml index cb82ec1f..fc7b9f0f 100644 --- a/db/candidates/Ascomycota/44080_gdd12_MATss_MATalpha/metadata.yaml +++ b/db/candidates/Ascomycota/44080_gdd12_MATss_MATa-MATalpha/metadata.yaml @@ -1,4 +1,4 @@ -record_id: 44080_gdd12_MATss_MATalpha +record_id: 44080_gdd12_MATss_MATa-MATalpha record_version: 1 taxonomy: taxid: 44080 @@ -14,6 +14,7 @@ organism: mating_type: locus_name: MATss idiomorphs: + - MATa - MATalpha system: homothallic locus: @@ -21,7 +22,7 @@ locus: excluded_from_coordinate_benchmark: false core: completeness: partial - reference_orientation: MATalpha1->MATalpha2->sla2 + reference_orientation: MATalpha2->MATalpha1->MATa1->sla2 definition_note: 'Left (alpha-dominant) sub-cluster of the active MAT locus on chromosome IV of S. schoenii GDD12 (CM143129.1; the paper''s span 470,610-475,000, Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC (Dost et al. 2024, PMID 38492364); @@ -30,23 +31,26 @@ locus: cites is not on its GitHub or Zenodo, so the models were rebuilt from the three public paired RNA-seq runs (PRJNA1364152; hisat2, StringTie, TransDecoder, phmmer against the curated MAT proteins, Pfam-A); see results/2026-10-29_ss_records/README.md. - The library is not stranded; strand is the ORF''s. MATalpha1 is single-exon, - minus strand, 191 aa. The homeodomain ORF (named MATalpha2 after the paper) - is partial (103 aa, two exons with a 217 bp intron of 198 reads that joins it - toward SLA2, the paper''s MAT-SLA2 splice junction); phmmer ranks it closest - to MATa1-type references (50 bits to C. albicans MTLA1), so the name is a hypothesis. - SLA2 here is a diverged copy: 256 aa (472,708-473,478, minus), 5-prime partial - (the ORF has no start codon); a second SLA2 piece (473,680-474,606, 309 aa) - is not recorded. An HMG-like ORF at 470,349-470,951 (200 aa, 44 bits to S. cerevisiae - MATA2, no Pfam hit) is not assigned to a gene. GENETIC CODE: NCBI table 12 (CUG - = Ser) for the species; proteins are translated with it.' + The library is not stranded; strand is the ORF''s. GENE NAMES come from new + MATa1, MATalpha2 and MATa2 profiles (results/2026-10-30_mat_hmms; supporting + evidence, a score difference, not a gate): the ORF at 470,349-470,951 (200 aa, + single exon, 227 aa with a 92 bp intron of 97 reads) is MATalpha2 (alpha2 profile + 73 bits, a1 12) and not an HMG-box MATa2; the homeodomain at 471,961-472,489 + is MATa1 (a1 86, alpha2 29), so the cluster holds both an alpha pair and an + a1 beside SLA2 (the paper''s MATa1-SLA2 junction). MATalpha1 is single-exon, + minus strand, 191 aa. The MATa1 model is partial (103 aa, two exons with a 217 + bp intron of 198 reads that joins it toward SLA2); the uninterrupted frame through + the intron scores lower (a1 60) and runs through a low-complexity repeat. SLA2 + is a diverged copy: 256 aa (472,708-473,478, minus), 5-prime partial (no start + codon); a second SLA2 piece (473,680-474,606, 309 aa) is not recorded. GENETIC + CODE: NCBI table 12 (CUG = Ser).' segments: - segment_index: 0 sequence_source: type: insdc_nucleotide accession: CM143129.1 seq_region: CM143129.1 - start: 471020 + start: 470349 end: 473478 contig_edge_distance: null sequence_checksum: null @@ -55,11 +59,25 @@ locus: assembly_accession_basis: strain_match_verified assembly_location: contig: CM143129.1 - start: 471020 + start: 470349 end: 473478 identity: 100.0 genes: - gene_index: 0 + name: MATalpha2 + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 470349 + end: 470951 + strand: + + order_in_locus: 0 + transl_table: 12 + exons: + - start: 470349 + end: 470951 +- gene_index: 1 name: MATalpha1 role: core_MAT present: true @@ -68,13 +86,13 @@ genes: start: 471020 end: 471595 strand: '-' - order_in_locus: 0 + order_in_locus: 1 transl_table: 12 exons: - start: 471020 end: 471595 -- gene_index: 1 - name: MATalpha2 +- gene_index: 2 + name: MATa1 role: core_MAT present: true locus_tag: null @@ -82,14 +100,14 @@ genes: start: 471961 end: 472489 strand: + - order_in_locus: 1 + order_in_locus: 2 transl_table: 12 exons: - start: 471961 end: 472227 - start: 472445 end: 472489 -- gene_index: 2 +- gene_index: 3 name: sla2 role: flanking_conserved present: true @@ -98,7 +116,7 @@ genes: start: 472708 end: 473478 strand: '-' - order_in_locus: 2 + order_in_locus: 3 transl_table: 12 exons: - start: 472708 @@ -121,9 +139,9 @@ evidence: citations: *id001 idiomorph_assignment: tier: 3 - experimental_method: the paper's assignment of the sub-cluster (alpha-dominant - left, palindromic a right); gene names follow it by position; phmmer ranks both - homeodomain ORFs closest to MATa1-type references, so MATalpha2 is a hypothesis + experimental_method: 'new MATa1, MATalpha2 and MATa2 profiles (a score difference, + supporting evidence: results/2026-10-30_mat_hmms); the sub-clusters are the + paper''s' citations: *id001 validation: status: needs_review @@ -138,7 +156,7 @@ validation: taxonomy_current: null curation: proposed_by: claude-code-curation-agent - proposal_dedupe_key: 41849659|44080|MATss|MATalpha + proposal_dedupe_key: 41849659|44080|MATss|MATa-MATalpha reviewed_by: null reviewed_date: null model_provenance: null diff --git a/db/candidates/Ascomycota/44080_gdd12_MATss_MATa/metadata.yaml b/db/candidates/Ascomycota/44080_gdd12_MATss_MATa/metadata.yaml index 0a86fe70..e52a5d2b 100644 --- a/db/candidates/Ascomycota/44080_gdd12_MATss_MATa/metadata.yaml +++ b/db/candidates/Ascomycota/44080_gdd12_MATss_MATa/metadata.yaml @@ -21,19 +21,22 @@ locus: excluded_from_coordinate_benchmark: false core: completeness: partial - reference_orientation: sla2->MATa1 + reference_orientation: sla2->MATa1->MATa2 definition_note: 'Right (palindromic) cassette of the active MAT locus on chromosome IV of S. schoenii GDD12 (CM143129.1; the paper''s span 528,044-537,777; an SLA2 anchor between mirrored MAT arrays, Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC (Dost et al. 2024, PMID 38492364). The record covers the arm that RNA-seq resolves: - SLA2 533,981-535,828 (plus, 615 aa, 5-prime partial: no start codon in the ORF) - and the homeodomain gene named MATa1 after the paper at 536,046-536,944 (minus, - 211 aa; introns of 205 bp, 221 reads, and 58 bp, 218 reads, the conserved 58 - bp junction the paper reports at 536,635). The mirrored arm (SLA2 529,992-532,490; - homeodomain ORF 529,258-529,749, 163 aa) is not recorded: short reads cannot - separate the two inverted copies, and the paper''s mirrored junction at 529,128 - is not seen. No HMG-box (MATa2) ORF passed the filters. GENE MODELS ARE OURS - (tier 3), see results/2026-10-29_ss_records/README.md. The library is not stranded. + SLA2 533,981-535,828 (plus, 615 aa, 5-prime partial: no start codon in the ORF), + MATa1 at 536,046-536,944 (minus, 211 aa; a1 profile 85 bits, alpha2 27; introns + of 205 bp, 221 reads, and 58 bp, 218 reads, the conserved 58 bp junction the + paper reports at 536,635) and MATa2 at 537,379-538,170 (plus, 263 aa, ATG to + stop, single exon, head-to-head with MATa1; a2 profile hit, results/2026-10-30_mat_hmms). + The a2 profile finds only three a2-like ORFs in the whole genome, all on chromosome + IV: this one, its mirror at 527,654-528,454 (minus) and one at 502,064-502,864 + at the central cluster. The mirrored arm (SLA2 529,992-532,490; MATa1 529,186-529,746) + is not recorded: short reads cannot separate the two inverted copies, and the + paper''s mirrored junction at 529,128 is not seen. GENE MODELS ARE OURS (tier + 3), see results/2026-10-29_ss_records/README.md. The library is not stranded. GENETIC CODE: NCBI table 12 (CUG = Ser).' segments: - segment_index: 0 @@ -42,7 +45,7 @@ locus: accession: CM143129.1 seq_region: CM143129.1 start: 533981 - end: 536944 + end: 538170 contig_edge_distance: null sequence_checksum: null extended_flank: [] @@ -51,7 +54,7 @@ locus: assembly_location: contig: CM143129.1 start: 533981 - end: 536944 + end: 538170 identity: 100.0 genes: - gene_index: 0 @@ -86,6 +89,20 @@ genes: end: 536634 - start: 536046 end: 536090 +- gene_index: 2 + name: MATa2 + role: core_MAT + present: true + locus_tag: null + segment_index: 0 + start: 537379 + end: 538170 + strand: + + order_in_locus: 2 + transl_table: 12 + exons: + - start: 537379 + end: 538170 evidence: locus_existence: tier: 2 @@ -104,9 +121,9 @@ evidence: citations: *id001 idiomorph_assignment: tier: 3 - experimental_method: the paper's assignment of the sub-cluster (alpha-dominant - left, palindromic a right); gene names follow it by position; phmmer ranks both - homeodomain ORFs closest to MATa1-type references, so MATalpha2 is a hypothesis + experimental_method: 'new MATa1, MATalpha2 and MATa2 profiles (a score difference, + supporting evidence: results/2026-10-30_mat_hmms); the sub-clusters are the + paper''s' citations: *id001 validation: status: needs_review diff --git a/results/2026-10-30_barnettozyma/36038_pb4207_MATbz_MATa-MATalpha.yaml b/results/2026-10-30_barnettozyma/36038_pb4207_MATbz_MATa-MATalpha.yaml index 4374c5e7..29766977 100644 --- a/results/2026-10-30_barnettozyma/36038_pb4207_MATbz_MATa-MATalpha.yaml +++ b/results/2026-10-30_barnettozyma/36038_pb4207_MATbz_MATa-MATalpha.yaml @@ -30,7 +30,7 @@ locus: identity: 100.0 core: completeness: partial - reference_orientation: DIC1->MATa2->MATa1->MATalpha1->SLA2 (MATalpha2 not modelled) + reference_orientation: DIC1->MATa2->MATa1->MATalpha1->MATalpha2 (partial)->SLA2 definition_note: 'DRAFT, GENE MODELS ARE OURS (tier 3). B. californica strain PB4207 (Dalian Medical University, GCA_023627795.1, ctg_5 = JAMAIE010000005.1, 694,392 bp; the locus is 63 kb from one contig end). The one published description is Hession, Byrne, Wolfe & Butler, PLoS Genet 2025 (PMID @@ -49,15 +49,16 @@ locus: MATa1 (162 aa, homeodomain, single exon), MATalpha1 (233 aa, alpha-box, zero-intron ORF whose C-terminus is uncertain: one-intron alternatives score the same), MATa2 (269 aa, one 56 bp GTATGT..CTTAG intron at 614,112-614,167, 82 bits to the MATa2 profile against a background maximum of 32 over all 124 - six-frame segments of the window; no Pfam HMG_box hit, as for Komagataella). MATalpha2 IS NOT RECORDED: - a homeodomain region (genome 620,370-620,700) follows MATalpha1 but no complete gene model could - be built; it is not recorded as present or absent. NAMING: Pfam cannot tell the MATa1 homeodomain - from the MATalpha2 homeodomain; the names follow position (the homeodomain gene next to the HMG-like - gene on the MATa side is MATa1). The other three californica assemblies (UCD09 GCA_003123585.1, - UCD89 GCA_003123885.1, NRRL Y-17395 GCA_003706655.3) carry the same proteins at 99.6-100% identity, - which is one strain family, not independent evidence. B. discipulorum (GCA_049863505.1) has SLA2, - MATa1, MATalpha1 and DIC1 at 45-86% identity but arranged differently and no MATa2 hit. GENETIC - CODE: standard (table 1), not tested. Details: results/2026-10-30_barnettozyma/README.md.' + six-frame segments of the window; no Pfam HMG_box hit, as for Komagataella). MATalpha2 IS RECORDED + AS PARTIAL (added 2026-10-30): a 113 aa alpha2-type homeodomain ORF at 620,080-620,421 (plus strand; + the alpha2 profile of results/2026-10-30_mat_hmms scores 37 bits), the same ORF in all four californica + assemblies; it begins right after an in-frame stop, so the N-terminal exon is not found and the + model is a fragment. NAMING: the a1 and alpha2 profiles of results/2026-10-30_mat_hmms (supporting + evidence, a score difference) call the MATa1 homeodomain a1 (104 bits against 23 for alpha2). The + other three californica assemblies (UCD09 GCA_003123585.1, UCD89 GCA_003123885.1, NRRL Y-17395 GCA_003706655.3) + carry the same proteins at 99.6-100% identity, which is one strain family, not independent evidence. + B. discipulorum (GCA_049863505.1) has SLA2, MATa1, MATalpha1 and DIC1 at 45-86% identity but arranged + differently and no MATa2 hit. GENETIC CODE: standard (table 1), not tested. Details: results/2026-10-30_barnettozyma/README.md.' segments: - segment_index: 0 sequence_source: @@ -122,6 +123,18 @@ genes: end: 619103 gene_class: alpha_box - gene_index: 4 + name: MATalpha2 + role: core_MAT + present: true + segment_index: 0 + start: 620080 + end: 620421 + strand: + + order_in_locus: 4 + exons: + - start: 620080 + end: 620421 +- gene_index: 5 name: sla2 role: flanking_conserved present: true @@ -129,7 +142,7 @@ genes: start: 627731 end: 630871 strand: '-' - order_in_locus: 4 + order_in_locus: 5 exons: - start: 627731 end: 630871 @@ -149,7 +162,8 @@ evidence: idiomorph_assignment: tier: 3 experimental_method: 'gene content and position: HMG-like MATa2 + homeodomain MATa1 on one side, alpha-box - MATalpha1 on the other, separated by 2.8 kb (PMID 41359681); homeodomain names by position only' + MATalpha1 on the other, separated by 2.8 kb (PMID 41359681); the a1 profile of results/2026-10-30_mat_hmms + calls the MATa1 homeodomain a1 (104 vs 23 bits); MATa2 scores 81-82 bits on the a2 profiles' citations: *id001 validation: status: needs_review diff --git a/results/2026-10-30_barnettozyma/make_records.py b/results/2026-10-30_barnettozyma/make_records.py index 9e6b5cdd..2802023b 100644 --- a/results/2026-10-30_barnettozyma/make_records.py +++ b/results/2026-10-30_barnettozyma/make_records.py @@ -13,8 +13,11 @@ gid = re.search(r"(?:ID|Parent)=(BC_[^;.]+)", c[8]).group(1) if c[2] == "gene": meta[gid] = (c[6], int(c[3]), int(c[4])) if c[2] == "CDS": exons[gid].append((int(c[3]), int(c[4]))) +# MATalpha2 (added 2026-10-30 from results/2026-10-30_mat_hmms): a partial alpha2 homeodomain ORF, 620,080-620,421 plus strand, 113 aa to the stop; +# the ORF begins right after an in-frame stop (620,077-620,079), so the N-terminal exon is not found. alpha2 profile 37 bits; same ORF in all four assemblies. +meta["BC_MATalpha2"] = ("+", 620080, 620421); exons["BC_MATalpha2"] = [(620080, 620421)] ROLE = {"dic1": "flanking_conserved", "sla2": "flanking_conserved"} -IDIO = {"MATa1": ["MATa"], "MATa2": ["MATa"], "MATalpha1": ["MATalpha"]} +IDIO = {"MATa1": ["MATa"], "MATa2": ["MATa"], "MATalpha1": ["MATalpha"], "MATalpha2": ["MATalpha"]} GCLASS = {"MATalpha1": "alpha_box"} order = sorted(meta, key=lambda g: meta[g][1]) genes = [] @@ -39,9 +42,7 @@ "(DIC1 7.4 kb from MATa2; SLA2 8.6 kb from MATalpha1). MODELS: SLA2 (1,046 aa, ANTH + I_LWEQ, single exon), DIC1 (316 aa, single exon), MATa1 (162 aa, " "homeodomain, single exon), MATalpha1 (233 aa, alpha-box, zero-intron ORF whose C-terminus is uncertain: one-intron alternatives score the same), MATa2 (269 aa, " "one 56 bp GTATGT..CTTAG intron at 614,112-614,167, 82 bits to the MATa2 profile against a background maximum of 32 over all 124 six-frame segments of the " - "window; no Pfam HMG_box hit, as for Komagataella). MATalpha2 IS NOT RECORDED: a homeodomain region (genome 620,370-620,700) follows MATalpha1 but no " - "complete gene model could be built; it is not recorded as present or absent. NAMING: Pfam cannot tell the MATa1 homeodomain from the MATalpha2 homeodomain; " - "the names follow position (the homeodomain gene next to the HMG-like gene on the MATa side is MATa1). The other three californica assemblies (UCD09 " + "window; no Pfam HMG_box hit, as for Komagataella). MATalpha2 IS RECORDED AS PARTIAL (added 2026-10-30): a 113 aa alpha2-type homeodomain ORF at 620,080-620,421 (plus strand; the alpha2 profile of results/2026-10-30_mat_hmms scores 37 bits), the same ORF in all four californica assemblies; it begins right after an in-frame stop, so the N-terminal exon is not found and the model is a fragment. NAMING: the a1 and alpha2 profiles of results/2026-10-30_mat_hmms (supporting evidence, a score difference) call the MATa1 homeodomain a1 (104 bits against 23 for alpha2). The other three californica assemblies (UCD09 " "GCA_003123585.1, UCD89 GCA_003123885.1, NRRL Y-17395 GCA_003706655.3) carry the same proteins at 99.6-100% identity, which is one strain family, not independent " "evidence. B. discipulorum (GCA_049863505.1) has SLA2, MATa1, MATalpha1 and DIC1 at 45-86% identity but arranged differently and no MATa2 hit. " "GENETIC CODE: standard (table 1), not tested. Details: results/2026-10-30_barnettozyma/README.md.") @@ -54,14 +55,14 @@ "locus": {"assembly_accession": ASM, "assembly_accession_basis": "strain_match_verified", "coordinate_provenance": "curator_derived", "excluded_from_coordinate_benchmark": False, "assembly_location": {"contig": CONTIG, "start": lo, "end": hi, "identity": 100.0}, - "core": {"completeness": "partial", "reference_orientation": "DIC1->MATa2->MATa1->MATalpha1->SLA2 (MATalpha2 not modelled)", "definition_note": NOTE, + "core": {"completeness": "partial", "reference_orientation": "DIC1->MATa2->MATa1->MATalpha1->MATalpha2 (partial)->SLA2", "definition_note": NOTE, "segments": [{"segment_index": 0, "sequence_source": {"type": "insdc_nucleotide", "accession": ACC, "seq_region": ACC}, "start": lo, "end": hi, "contig_edge_distance": None, "sequence_checksum": None}]}, "extended_flank": []}, "genes": genes, "evidence": {"locus_existence": {"tier": 3, "experimental_method": "published description of the locus (PMID 41359681) plus our own homology/ORF analysis of the PB4207 assembly; no RNA-seq", "citations": CIT}, "boundaries": {"tier": 3, "experimental_method": "gene models predicted here from sequence (ORF, Pfam, phmmer, one-intron search); not an annotation of the deposit", "citations": []}, - "idiomorph_assignment": {"tier": 3, "experimental_method": "gene content and position: HMG-like MATa2 + homeodomain MATa1 on one side, alpha-box MATalpha1 on the other, separated by 2.8 kb (PMID 41359681); homeodomain names by position only", "citations": CIT}}, + "idiomorph_assignment": {"tier": 3, "experimental_method": "gene content and position: HMG-like MATa2 + homeodomain MATa1 on one side, alpha-box MATalpha1 on the other, separated by 2.8 kb (PMID 41359681); the a1 profile of results/2026-10-30_mat_hmms calls the MATa1 homeodomain a1 (104 vs 23 bits); MATa2 scores 81-82 bits on the a2 profiles", "citations": CIT}}, "validation": {"status": "needs_review", "rejection_reason": None, "accession_resolved": False, "accession_resolved_date": None, "accession_resolved_version": None, "sequence_match": {"status": "pass", "per_gene": [], "notes": "models derived from the assembly itself"}, "taxonomy_current": None}, "curation": {"proposed_by": "claude-code-curation-agent", "proposal_dedupe_key": "41359681|36038|MATbz|MATa-MATalpha|PB4207", "reviewed_by": None, "reviewed_date": None}, diff --git a/results/2026-10-30_mat_hmms/.gitignore b/results/2026-10-30_mat_hmms/.gitignore new file mode 100644 index 00000000..e2ccc09c --- /dev/null +++ b/results/2026-10-30_mat_hmms/.gitignore @@ -0,0 +1,3 @@ +work/ +__pycache__/ +*.log diff --git a/results/2026-10-30_mat_hmms/README.md b/results/2026-10-30_mat_hmms/README.md new file mode 100644 index 00000000..4c776dd3 --- /dev/null +++ b/results/2026-10-30_mat_hmms/README.md @@ -0,0 +1,46 @@ +# MATa2 support and a1 / alpha2 profiles (2026-10-30) + +Question (curator): (a) is there support for the MATa2 genes of the new tier-3 records, (b) can profiles tell MATa1 from MATalpha2 (both homeodomain), and how many copies are needed. + +## Data +`inventory.py`, `fetch_public.py`, `fetch_public2.py`, `build_sets.py` collect the proteins: the curated `db/` Saccharomycotina proteins plus titled NCBI Protein records (taxid 147537), **one per species per class**, mislabeled/partial/hypothetical removed (`data/set_meta.tsv`). +**a1 29 species, alpha2 25, a2 14, alpha1 12** (Saccharomycetaceae, Serinales, Pichiaceae, Dipodascales; Saccharomycetaceae dominates alpha2: 17 of 25). Profiles: mafft `--localpair --maxiterate 1000`, pyhmmer `Builder` (seed 42); `loco.py`, `a2_loo.py`, `ablation.py` run on the HPCC; `hmm/*.hmm` are the results. Pfam is `pfam_scan.py`; scoring is `score_tests.py`. + +## (b) a1 vs alpha2: the data support separate profiles +- **Leave-one-clade-out** (`data/loco.tsv`): 53 of 54 correct; leave-one-species-out also 53 of 54. Typical margin (own profile minus the other) 65 bits for a1 and 75 for alpha2; the second-lowest margin is 27 bits for both. +- The one error is a labelling error, not a model error: *Hyphopichia burtonii* XP_020074339.1 "Mtla1p" scores a1 19 vs alpha2 115 (likely an NCBI annotation error). A second near-zero case (*Ogataea thermomethanolica* QFP92360.1 "ALPHA2", 0.0 vs 0.0) is probably truncated. +- **Copies needed** (`data/ablation.tsv`, 8 random draws per k): accuracy on the held-out rest is 97-98% already with **2 sequences per class** and does not rise with more; the residual 2% is those two sequences. So the classes are separable with few copies; what limits use is clade coverage (alpha2 is nearly all Saccharomycetaceae and Pichiaceae, only 2 Serinales; no Ascoideales/Phaffomycetales/Dipodascales alpha2), not copy number. +- Verdict: **build them** (`hmm/a1.hmm`, `hmm/alpha2.hmm`; a2 and alpha1 too), as supporting evidence, referenced by no gate until the curator decides, like the MATa2 profile. Report the score difference, not a hard call. + +## Scoring our ORFs (`data/test_scores.tsv`, `data/test_rank.tsv`; background = the S. schoenii proteome, 3,067 proteins) +| protein | a1 | alpha2 | a2 | alpha1 | reading | +|---|---|---|---|---|---| +| P. fabianii MATa1 | 111 | 9 | | | a1 | +| B. californica MATa1 | 104 | 23 | | | a1 | +| S. schoenii right homeodomain 536,046-536,944 | 85 | 27 | | | a1 (MATa1, as recorded) | +| S. schoenii mirrored copy 529,258-529,749 | 60 | 20 | | | a1 | +| **S. schoenii left homeodomain p5 (471,961-472,489)** | 86 | 29 | | | **a1, not alpha2** | +| **S. schoenii "HMG-like" ORF p3 (470,349-470,951)** | 9 | **81** | -2 | | **alpha2, not a2** | +| B. californica 620,080-620,418 (113 aa, frame +2) | 4 | **37** | | | alpha2 (partial homeodomain) | + +## (a) MATa2 support +- **a2 profile** (14 sequences; `hmm/a2.hmm`) and the existing Saccharomycotina MATa2 profile, background maximum 19 and 17 bits: + - *P. fabianii* MATa2 **73 / 77 bits**; *B. californica* PB4207 MATa2 **81 / 82**; both rank above every background protein. The *P. kudriavzevii* control scores 167 (in training), **43.5 with Pichiaceae held out** (167 with Saccharomycetaceae held out; the candidates score 76 / 76 and 78 / 81 with Saccharomycetaceae / Pichiaceae held out, `hmm/a2_no_*`), so the profile finds a Pichiaceae a2 it has not seen. + - Pfam HMG_box is below GA for all three (13.5, 14.5, control 18.4; GA 25.4), the same weakness as for *Komagataella*; the profile is the better evidence. + - Synteny: MATa2 and MATa1 are divergent neighbours, 5'-5', 574 bp apart in *P. fabianii* and 625 bp in *B. californica* (275 bp in *P. kudriavzevii*). + - Conservation: the *B. californica* a2 exon-1 segment scores 41.2 in all four assemblies; *B. discipulorum* has no a2 above 15 by a stop-to-stop scan (introns not modelled), as the earlier agent found. +- **S. schoenii: the HMG-like ORF in the left cluster is not MATa2; it is MATalpha2** (alpha2 profile 81 / 73 bits, a1 9-12, a2 negative; weak Pfam Homeodomain 16 bits). **The real MATa2 is in the right cassette and was missed** because the StringTie transcripts there are thin: whole-genome six-frame scan (`ss_a2_scan.py`, `data/ss_genome_scan.tsv`) finds only **three** a2-like ORFs in the genome, all on chromosome IV, each 800 nt open, a2 profile 77.6 / Saccharomycotina profile 66: + - **CM143129.1:527,654-528,454 (minus)** and its mirror **537,367-538,167 (plus)**, the outer ends of the palindrome, each head-to-head with an a1 homeodomain (529,186-529,746 plus; 536,074-536,634 minus; gaps 732 and 423 bp); + - **502,064-502,864 (minus)**, with an a1 at 503,643-504,203 (the central cluster at the centromere). +- Each of the six chromosome cassettes (CM143126-31) carries one alpha2 (71-76 bits), one alpha1 (115) and one a1-like homeodomain (59-61); a2 occurs only on chromosome IV. +- Literature naming (According to PubMed): Hanson, Byrne and Wolfe, PNAS 2014 ([10.1073/pnas.1416014111](https://doi.org/10.1073/pnas.1416014111), PMID 25349420) name MATa2 and MATalpha2 as the genes repressed beside a telomere or centromere in *Pichia pastoris* and *Hansenula polymorpha*, supporting a2/alpha2 as the second gene of each idiomorph. + +## Record changes recommended (not applied; db/ untouched) +1. **S. schoenii left record**: rename the 470,349-470,951 ORF (200 aa; 227 aa with the 92 bp intron, 97 reads) **MATalpha2** and the homeodomain at 471,889-472,461 **MATa1** (single exon, 190 aa, uninterrupted; the 217 bp junction at 472,228-472,444 is a read-through isoform toward SLA2, not the gene's intron, so the 103 aa two-exon model should go). The recorded "MATalpha2 = p5" is wrong by homology. Re-check the right-cassette MATa1 intron model (536,074-536,634 is open without the 205 bp intron). +2. **S. schoenii right record**: add **MATa2** at 537,367-538,167 (plus) (mirror copy 527,654-528,454); the family declares MATa2 optional, so this is allowed. +3. **B. californica**: add a partial **MATalpha2** (620,080-620,418, plus, 113 aa, alpha2 profile 37 bits, homeodomain residues 63-155 of the profile, conserved in all four assemblies, 26.5 bits in *B. discipulorum*); keep MATa2 (81 bits). +4. **P. fabianii**: MATa2 supported (73-77 bits); no change. Still no MATalpha2 in the alpha allele: the alpha2 profile finds nothing (could be tested with `hmm/alpha2.hmm` on the TBRC 4498 window). +5. Gene names `MATa1` / `MATalpha2` need only the new profile score difference as evidence, stated as supporting. + +## Limits +Public a1/alpha2 sequences are mostly Saccharomycetaceae; titles are NCBI's own (one mislabel found). The S. schoenii background is itself full of MAT paralogs (alpha2 background maximum is the p3 paralog on another chromosome). Strand and exon structures of the S. schoenii ORFs are unmodelled in `ss_a2_scan.py` (uninterrupted segments only). No record was edited; no commit was made. diff --git a/results/2026-10-30_mat_hmms/a2_loo.py b/results/2026-10-30_mat_hmms/a2_loo.py new file mode 100644 index 00000000..de5c917d --- /dev/null +++ b/results/2026-10-30_mat_hmms/a2_loo.py @@ -0,0 +1,9 @@ +"""a2 profiles with a clade held out (the control and the tier-3 genes are then truly external to training). HPCC (mafft).""" +import sys +sys.argv = ["x"] +exec(open("loco.py").read().split("CL = [")[0]) # reuse seqs, build, score +for hold in ("Pichiaceae", "Saccharomycetaceae", "Serinales"): + items = [(i, v[3]) for i, v in seqs.items() if v[0] == "a2" and v[2] != hold] + h = build("a2_no_" + hold, items) + with open(f"hmm/a2_no_{hold}.hmm", "wb") as fh: h.write(fh) + print(hold, len(items), h.M) diff --git a/results/2026-10-30_mat_hmms/a2_loo.slurm b/results/2026-10-30_mat_hmms/a2_loo.slurm new file mode 100644 index 00000000..0419e6af --- /dev/null +++ b/results/2026-10-30_mat_hmms/a2_loo.slurm @@ -0,0 +1,8 @@ +#!/bin/bash +#SBATCH -p exfab -A exfab -c 2 --mem 8G -t 01:00:00 -J mat-a2loo +#SBATCH -o /bigdata/stajichlab/jstajich/projects/MATPredict/logs/mat_a2loo_%j.log +set -euo pipefail +set +eu; source /etc/profile 2>/dev/null; module load mafft/7.505; set -eu; unset MAFFT_BINARIES +export PATH=/bigdata/stajichlab/jstajich/projects/MATPredict/.pixi/envs/default/bin:$PATH +cd /bigdata/stajichlab/jstajich/projects/MATPredict/results/2026-10-30_mat_hmms +python a2_loo.py diff --git a/results/2026-10-30_mat_hmms/a2_orf.py b/results/2026-10-30_mat_hmms/a2_orf.py new file mode 100644 index 00000000..ff3515f9 --- /dev/null +++ b/results/2026-10-30_mat_hmms/a2_orf.py @@ -0,0 +1,16 @@ +import sys +sys.argv=['x'] +exec(open('score_models.py').read().split('MODELS =')[0]) +def stop_down(k): + while codon[sub(k,k+2)]!='*': k+=3 + return k+2 +def stop_up(k): + while True: + k-=3 + if codon[sub(k,k+2)]=='*': return k +for start in (537367,): + e=stop_down(start); u=stop_up(start) + j=u+3 + while sub(j,j+2)!='ATG': j+=3 + print('a2 plus: upstream stop',u,'first ATG',j,'stop ends',e,'aa',(e-j+1)//3-1) + print(tr(sub(j,e))) diff --git a/results/2026-10-30_mat_hmms/ablation.py b/results/2026-10-30_mat_hmms/ablation.py new file mode 100644 index 00000000..99d138f9 --- /dev/null +++ b/results/2026-10-30_mat_hmms/ablation.py @@ -0,0 +1,18 @@ +"""How many copies are needed: random k sequences per class (k = 2..20, 8 draws) build the a1 and alpha2 profiles; accuracy on all other a1/alpha2 sequences. HPCC (mafft).""" +import random, sys +sys.argv = ["x"] +exec(open("loco.py").read().split("CL = [")[0]) +random.seed(7) +a1 = [i for i, v in seqs.items() if v[0] == "a1"]; al2 = [i for i, v in seqs.items() if v[0] == "alpha2"] +out = open("data/ablation.tsv", "w"); out.write("k\tdraw\tn_test\tcorrect\tacc\tmin_margin\n") +for k in (2, 3, 4, 6, 8, 12, 18): + for d in range(8): + tr1 = random.sample(a1, k); tr2 = random.sample(al2, k) + h1 = build("a1", [(i, seqs[i][3]) for i in tr1]); h2 = build("alpha2", [(i, seqs[i][3]) for i in tr2]) + ok = n = 0; mm = 999 + for i, v in seqs.items(): + if v[0] in ("a1", "alpha2") and i not in tr1 and i not in tr2: + s1, s2 = score(h1, v[3]), score(h2, v[3]); n += 1 + good = (s1 > s2) == (v[0] == "a1"); ok += good + mm = min(mm, (s1 - s2) if v[0] == "a1" else (s2 - s1)) + out.write(f"{k}\t{d}\t{n}\t{ok}\t{ok / n:.3f}\t{mm:.1f}\n"); out.flush() diff --git a/results/2026-10-30_mat_hmms/ablation.slurm b/results/2026-10-30_mat_hmms/ablation.slurm new file mode 100644 index 00000000..da45b1da --- /dev/null +++ b/results/2026-10-30_mat_hmms/ablation.slurm @@ -0,0 +1,9 @@ +#!/bin/bash +#SBATCH -p exfab -A exfab -c 2 --mem 8G -t 03:00:00 -J mat-abl +#SBATCH -o /bigdata/stajichlab/jstajich/projects/MATPredict/logs/mat_abl_%j.log +set -euo pipefail +set +eu; source /etc/profile 2>/dev/null; module load mafft/7.505; set -eu; unset MAFFT_BINARIES +export PATH=/bigdata/stajichlab/jstajich/projects/MATPredict/.pixi/envs/default/bin:$PATH +cd /bigdata/stajichlab/jstajich/projects/MATPredict/results/2026-10-30_mat_hmms +python ablation.py +touch LOCO_DONE diff --git a/results/2026-10-30_mat_hmms/bc_alpha2_scan.py b/results/2026-10-30_mat_hmms/bc_alpha2_scan.py new file mode 100644 index 00000000..7ae7e926 --- /dev/null +++ b/results/2026-10-30_mat_hmms/bc_alpha2_scan.py @@ -0,0 +1,57 @@ +"""Six-frame segments (stop to stop) of B. californica PB4207 JAMAIE010000005.1 618,500-623,500 and of the other three californica assemblies' +homologous region scored with the alpha2, a1 and alpha1 profiles; coordinates 1-based.""" +import itertools, pyhmmer, sys +from pyhmmer.easel import Alphabet, TextSequence +CODON = {a + b + c: aa for (a, b, c), aa in zip(itertools.product("TCAG", repeat=3), "FFLLSSSSYY**CC*WLLLLPPPPHHQQRRRRIIIMTTTTNNKKSSRRVVVVAAAADDEEGGGG")} +def rc(s): return s[::-1].translate(str.maketrans("ACGTacgt", "TGCAtgca")) +def tr(s): return "".join(CODON.get(s[i:i + 3].upper(), "X") for i in range(0, len(s) - 2, 3)) +def fasta(p): + d = {}; n = None + for l in open(p): + if l.startswith(">"): n = l[1:].split()[0]; d[n] = "" + elif n: d[n] += l.strip() + return d +alpha = Alphabet.amino(); H = {} +for n in ("alpha2", "a1", "alpha1"): + with pyhmmer.plan7.HMMFile(f"hmm/{n}.hmm") as hf: H[n] = hf.read() +def segs(seq, a, b, off): + sub = seq[a - off:b - off + 1]; out = [] + for strand, s in (("+", sub), ("-", rc(sub))): + for f in range(3): + p = tr(s[f:]); pos = 0 + for seg in p.split("*"): + if len(seg) >= 40: + n0 = f + 3 * pos; n1 = n0 + 3 * len(seg) - 1 + g0, g1 = (a + n0, a + n1) if strand == "+" else (b - n1, b - n0) + out.append((f"{strand}{f}:{g0}-{g1}", seg)) + pos += len(seg) + 1 + return out +def score(seg): + q = [TextSequence(name=k.encode(), sequence=v).digitize(alpha) for k, v in seg] + res = {k: {} for k, _ in seg} + for n, h in H.items(): + for hits in pyhmmer.hmmsearch([h], q, E=1e9, domE=1e9, F1=1.0, F2=1.0, F3=1.0, bias_filter=False): + for x in hits: res[str(x.name)][n] = x.score + return res +D = "../2026-10-30_barnettozyma/data/" +for fa, off, a, b in ((D + "GCA_023627795.1_JAMAIE010000005.1_589225-655853.fa", 589225, 618500, 623500),): + seq = next(iter(fasta(fa).values())); s = segs(seq, a, b, off); r = score(s) + top = sorted(r.items(), key=lambda kv: -max(kv[1].values() or [0]))[:6] + for k, v in top: print(k, len(dict(s)[k]), {n: round(x, 1) for n, x in v.items()}) +print("--- alignment span of the best alpha2 segment, and the same search in the other californica windows") +seqs_ = dict(s) +best = max(r.items(), key=lambda kv: kv[1].get("alpha2", -9))[0] +q = [TextSequence(name=best.encode(), sequence=seqs_[best]).digitize(alpha)] +for hits in pyhmmer.hmmsearch([H["alpha2"]], q, E=1e9, domE=1e9, F1=1.0, F2=1.0, F3=1.0, bias_filter=False): + for x in hits: + for d in x.domains: + a = d.alignment + print(best, "hmm", a.hmm_from, a.hmm_to, "of", H["alpha2"].M, "target", a.target_from, a.target_to, "of", len(seqs_[best]), round(d.score, 1)) + print(seqs_[best][:80], "... starts with", seqs_[best][:12]) +for fa in sorted(__import__("glob").glob(D + "GCA_*_*.fa")): + seq = next(iter(fasta(fa).values())); sg = segs(seq, 1, len(seq), 1) + # keep segments >= 90 aa to limit work + sg = [(k, v) for k, v in sg if len(v) >= 90] + rr = score(sg) + topk = sorted(rr.items(), key=lambda kv: -kv[1].get("alpha2", -9))[:2] + print(fa.split("/")[-1][:30], [(k, len(dict(sg)[k]), round(v.get("alpha2", 0), 1), round(v.get("a1", 0), 1)) for k, v in topk]) diff --git a/results/2026-10-30_mat_hmms/build_sets.py b/results/2026-10-30_mat_hmms/build_sets.py new file mode 100644 index 00000000..886096f1 --- /dev/null +++ b/results/2026-10-30_mat_hmms/build_sets.py @@ -0,0 +1,49 @@ +"""Training sets for a1 / alpha2 / a2 / alpha1 profiles: curated db proteins plus titled NCBI public proteins, one per species per class.""" +import re, collections +TAXON = {"4952": ("Yarrowia lipolytica", "Dipodascales"), "1005962": ("Ogataea polymorpha", "Pichiaceae"), "460523": ("Ogataea parapolymorpha", "Pichiaceae"), + "460519": ("Komagataella phaffii", "Pichiaceae"), "4909": ("Pichia kudriavzevii", "Pichiaceae"), "28985": ("Kluyveromyces lactis", "Saccharomycetaceae"), + "4932": ("Saccharomyces cerevisiae", "Saccharomycetaceae"), "4929": ("Saccharomyces cerevisiae", "Saccharomycetaceae"), "381046": ("Lachancea kluyveri", "Saccharomycetaceae"), + "4959": ("Debaryomyces hansenii", "Serinales"), "36911": ("Clavispora lusitaniae", "Serinales"), "5476": ("Candida albicans", "Serinales"), + "498019": ("Candida auris", "Serinales"), "42374": ("Candida auris", "Serinales")} +SACC = "Monosporozyma Saccharomyces Zygosaccharomyces Kluyveromyces Lachancea Nakaseomyces Kazachstania Tetrapisispora Maudiozyma Naumovozyma Torulaspora Eremothecium Vanderwaltozyma Eremothecium Ashbya Zygotorulaspora Kluyveromyces".split() +SER = "Candidozyma Meyerozyma Candida Clavispora Debaryomyces Meyerozyma Scheffersomyces Lodderomyces Spathaspora Millerozyma Priceomyces Wickerhamiella".split() +PICH = "Hyphopichia Ogataea Komagataella Pichia Kuraishia".split() +def clade(genus): + if genus in SACC: return "Saccharomycetaceae" + if genus in PICH: return "Pichiaceae" + if genus in ("Yarrowia", "Geotrichum", "Dipodascus"): return "Dipodascales" + if genus in SER: return "Serinales" + return "other" +import glob, yaml +def species_of(rec): + f = glob.glob(f"../../db/Ascomycota/*/{rec}/metadata.yaml")[0] + d = yaml.safe_load(open(f)); sp = d["organism"]["species"] + return sp, clade(sp.split()[0]) +rows = {} # (class, species) -> (id, seq, clade, source) +def add(cls, species, ident, seq, cl, src): + k = (cls, species) + if k not in rows or (src == "curated" and rows[k][3] != "curated") or (src == rows[k][3] and len(seq) > len(rows[k][1])): + rows[k] = (ident, seq, cl, src) +for l in open("data/curated_mat_proteins.faa").read().split(">")[1:]: + h, *s = l.split("\n"); rec, name, c = h.split("|"); sp, cl = species_of(rec) + add(c, sp, f"{rec}|{name}", "".join(s), cl, "curated") +PAT = {"a1": re.compile(r"(mat\s?-?a1|mating[- ]type (protein )?a1|\ba1\b.*transcription|a1p|\bA1 transcription)", re.I), + "alpha2": re.compile(r"(alpha2|mat\s?-?alpha2)", re.I), "a2": re.compile(r"(mat\s?-?a2|mating[- ]type (protein )?a2|mata2)", re.I)} +for c in ("a1", "alpha2", "a2"): + for l in open(f"data/public_{c}.faa").read().split(">")[1:]: + h, *s = l.split("\n"); seq = "".join(s) + m = re.match(r"(\S+) (.*) \[(.*?)\]\s*$", h) + if not m or h.startswith("pdb") or "hypothetical" in h or "homeobox" in h.lower() or "partial" in h or "homeodomain" in h.lower() and c == "a2": continue + acc, title, sp = m.groups(); sp = re.sub(r"\s+(CBS|IFO|NBRC|CD|S288C|DMKU).*$", "", sp.strip("[] ")) + sp = " ".join(sp.replace("[", "").replace("]", "").split()[:2]) + if not PAT[c].search(title) or (c != "alpha2" and re.search(r"alpha", title, re.I)): continue + if c == "a1" and re.search(r"alpha", title, re.I): continue + add(c, sp, acc + "|" + title[:40].replace(" ", "_"), seq, clade(sp.split()[0].strip("[]")), "ncbi") +out = collections.defaultdict(list) +with open("data/set_meta.tsv", "w") as m: + m.write("class\tspecies\tclade\tsource\tid\tlen\n") + for (c, sp), (i, s, cl, src) in sorted(rows.items()): + out[c].append((i, s)); m.write(f"{c}\t{sp}\t{cl}\t{src}\t{i}\t{len(s)}\n") +for c, v in out.items(): + open(f"data/set_{c}.faa", "w").write("".join(f">{i.replace(' ', '_')}\n{s}\n" for i, s in v)) + print(c, len(v), collections.Counter(rows[(c, sp)][2] for (cc, sp) in rows if cc == c)) diff --git a/results/2026-10-30_mat_hmms/bz_windows_scan.py b/results/2026-10-30_mat_hmms/bz_windows_scan.py new file mode 100644 index 00000000..91227867 --- /dev/null +++ b/results/2026-10-30_mat_hmms/bz_windows_scan.py @@ -0,0 +1,17 @@ +"""All five Barnettozyma windows (four B. californica assemblies, B. discipulorum): best stop-to-stop segment per profile (a2, a1, alpha2, alpha1).""" +import glob, sys +sys.argv = ["x"] +src = open("bc_alpha2_scan.py").read().split('D = "../2026')[0] +exec(src) +for n in ("a2", "sacch_a2"): + p = "../../db/Ascomycota/classifiers/MATa2_saccharomycotina/MATa2_saccharomycotina.hmm" if n == "sacch_a2" else "hmm/a2.hmm" + with pyhmmer.plan7.HMMFile(p) as hf: H[n] = hf.read() +D = "../2026-10-30_barnettozyma/data/" +out = open("data/bz_windows_scan.tsv", "w"); out.write("window\tprofile\tbest_segment\tlen\tbits\n") +for fa in sorted(glob.glob(D + "GCA_*_*.fa")): + seq = next(iter(fasta(fa).values())); sg = [(k, v) for k, v in segs(seq, 1, len(seq), 1) if len(v) >= 90] + rr = score(sg) + for pr in ("a2", "sacch_a2", "a1", "alpha2", "alpha1"): + k, v = max(rr.items(), key=lambda kv: kv[1].get(pr, -9)) + line = f"{fa.split('/')[-1][:28]}\t{pr}\t{k}\t{len(dict(sg)[k])}\t{v.get(pr, 0):.1f}" + print(line); out.write(line + "\n") diff --git a/results/2026-10-30_mat_hmms/data/ablation.tsv b/results/2026-10-30_mat_hmms/data/ablation.tsv new file mode 100644 index 00000000..c43d8c8c --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/ablation.tsv @@ -0,0 +1,57 @@ +k draw n_test correct acc min_margin +2 0 50 49 0.980 -64.6 +2 1 50 48 0.960 -70.9 +2 2 50 49 0.980 -36.3 +2 3 50 49 0.980 0.0 +2 4 50 48 0.960 -70.1 +2 5 50 49 0.980 -99.4 +2 6 50 49 0.980 -39.2 +2 7 50 49 0.980 -41.5 +3 0 48 47 0.979 -61.1 +3 1 48 47 0.979 -59.5 +3 2 48 47 0.979 -60.4 +3 3 48 48 1.000 0.0 +3 4 48 47 0.979 -60.4 +3 5 48 47 0.979 -75.5 +3 6 48 47 0.979 -61.3 +3 7 48 47 0.979 -54.2 +4 0 46 45 0.978 -88.8 +4 1 46 45 0.978 -76.8 +4 2 46 45 0.978 -82.0 +4 3 46 45 0.978 -77.9 +4 4 46 45 0.978 -82.1 +4 5 46 45 0.978 -66.7 +4 6 46 45 0.978 -98.1 +4 7 46 45 0.978 -65.1 +6 0 42 41 0.976 -68.9 +6 1 42 41 0.976 -75.1 +6 2 42 41 0.976 -79.0 +6 3 42 41 0.976 -91.5 +6 4 42 41 0.976 -90.2 +6 5 42 42 1.000 0.0 +6 6 42 41 0.976 -83.1 +6 7 42 41 0.976 -81.9 +8 0 38 37 0.974 -92.1 +8 1 38 37 0.974 -78.7 +8 2 38 38 1.000 9.5 +8 3 38 38 1.000 19.5 +8 4 38 38 1.000 0.0 +8 5 38 37 0.974 -95.0 +8 6 38 37 0.974 -94.5 +8 7 38 37 0.974 -89.0 +12 0 30 29 0.967 -102.2 +12 1 30 29 0.967 -94.9 +12 2 30 30 1.000 19.5 +12 3 30 30 1.000 19.4 +12 4 30 29 0.967 -93.1 +12 5 30 29 0.967 -91.7 +12 6 30 29 0.967 -96.0 +12 7 30 29 0.967 -91.2 +18 0 18 18 1.000 25.7 +18 1 18 17 0.944 -102.2 +18 2 18 18 1.000 39.0 +18 3 18 17 0.944 -92.8 +18 4 18 17 0.944 -92.1 +18 5 18 17 0.944 -103.0 +18 6 18 18 1.000 16.0 +18 7 18 18 1.000 27.6 diff --git a/results/2026-10-30_mat_hmms/data/bz_windows_scan.tsv b/results/2026-10-30_mat_hmms/data/bz_windows_scan.tsv new file mode 100644 index 00000000..4101f2f8 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/bz_windows_scan.tsv @@ -0,0 +1,26 @@ +window profile best_segment len bits +GCA_003123585.1_QBLI01000035 a2 -0:9116-9634 173 41.2 +GCA_003123585.1_QBLI01000035 sacch_a2 -1:9682-10035 118 32.2 +GCA_003123585.1_QBLI01000035 a1 +0:10579-11088 170 104.2 +GCA_003123585.1_QBLI01000035 alpha2 +0:15604-15942 113 37.4 +GCA_003123585.1_QBLI01000035 alpha1 +2:13923-14624 234 85.9 +GCA_003123885.1_QBLJ01000026 a2 +1:41744-42262 173 41.2 +GCA_003123885.1_QBLJ01000026 sacch_a2 +2:41343-41696 118 32.1 +GCA_003123885.1_QBLJ01000026 a1 -2:40290-40799 170 104.2 +GCA_003123885.1_QBLJ01000026 alpha2 -1:35437-35775 113 37.4 +GCA_003123885.1_QBLJ01000026 alpha1 -0:36755-37456 234 85.9 +GCA_003706655.3_PPLX03000005 a2 +1:41747-42265 173 41.3 +GCA_003706655.3_PPLX03000005 sacch_a2 +2:41346-41699 118 32.1 +GCA_003706655.3_PPLX03000005 a1 -1:40293-40802 170 104.2 +GCA_003706655.3_PPLX03000005 alpha2 -0:35440-35778 113 37.4 +GCA_003706655.3_PPLX03000005 alpha1 -2:36758-37462 235 85.9 +GCA_023627795.1_JAMAIE010000 a2 -0:24369-24887 173 41.2 +GCA_023627795.1_JAMAIE010000 sacch_a2 -1:24935-25288 118 32.1 +GCA_023627795.1_JAMAIE010000 a1 +1:25832-26341 170 104.3 +GCA_023627795.1_JAMAIE010000 alpha2 +0:30856-31194 113 37.4 +GCA_023627795.1_JAMAIE010000 alpha1 +2:29172-29876 235 85.9 +GCA_049863505.1_CM113344.1_1 a2 -0:29079-29849 257 14.6 +GCA_049863505.1_CM113344.1_1 sacch_a2 +0:22429-22842 138 5.8 +GCA_049863505.1_CM113344.1_1 a1 -2:28243-28605 121 108.1 +GCA_049863505.1_CM113344.1_1 alpha2 +1:32849-33139 97 26.5 +GCA_049863505.1_CM113344.1_1 alpha1 -0:29079-29849 257 89.2 diff --git a/results/2026-10-30_mat_hmms/data/curated_mat_proteins.faa b/results/2026-10-30_mat_hmms/data/curated_mat_proteins.faa new file mode 100644 index 00000000..595a5f48 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/curated_mat_proteins.faa @@ -0,0 +1,86 @@ +>4952_w29_MATyl_A|mata1|a1 +MPSRTPTDIWRCQRLILAARKGETTCQALHEQSIEISSSLKWFEEISEWMSKFPESGLYISTTIWTFLEGVPNEHRIKYDRLIRHYITYIQQLVPARLVTAYLDSLFKICSHPTRTERRIIGEHCGISLHQVTQWFTNRRFREPKTDSPDDGSTRFIPSPMTSPGSIDECSFPESP +>4952_w29_MATyl_A|mata2|a2 +MENTILHIHSFQLPQTEQPYPEAMLFDRDTSDSRTVLTQKPNGLEISLNFLQYDGHKGLFIRQDSRTNEPEYIEPKVLKPRNSYILFRNATSRCSQSIDPNSAFVSRVSSYIWGSGIPNPIVRRWFKYMGFFEALYHEVDYPEYIPNKLPKSPKKPKVQKGATKSKKKGAKGKNKVTALQVLVGPTVGVGGRMTVSPMTAFFSNNSHVTCYDPNFVLDTPTREFLMMPLDDITLPSQGPRSDSQEQHVPRQPPDGQDYFDILDIDDFVSPYDDLTTRSFTNRQLFKHATLG +>1005962_dl-1_MATog_MATa-MATalpha|MATa1|a1 +MQFIILNEPSLDSQRREGNLASENCVFARDIRKEGVKVLEDSLRSERNALLAIGAGGAVDRVERHYRRLLDGWEAAIMEIRSNDKYESLWRGFAIEKAEVETEMRKVEKQWPGELDVYKECFVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>1005962_dl-1_MATog_MATa-MATalpha|MATalpha1|alpha1 +MFLAFFVIARVGLQNLCLQRKFPPHGDSAKMALDGLNLSFLTHLPQIPTTSPLLKRLLSEYGAIDNGNDSSNKVAPKKSRRHRVQKQKKRLNGFMAFRSFYSRNIANYNSQKALSQDLADAWNREKHQQIWSLYAIQYNHSKSKEPFSIWLEMKLNKKLRKNNSDDPTTRREINIMSDIVVEDVFQECSS +>1005962_dl-1_MATog_MATa-MATalpha|MATalpha2|alpha2 +MSSYEEQCSAPDLLLLLDRLIDSITESDLSLEDELYWDSHVHSLMAVITHTVSSPSGTPMDDMLGQIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKFCKSSMESFTTERTIEYTRDCPPYAAVTTSQPGYKTEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTDCSPNDVV +>460519_cbs-7435_MATkp_MATa|MATa2|a2 +MTVTTFLSSSFDSVAFAMTCVSDKSYKTTAGKGLSGKKFNLSTHRPRNKFIISKSIFSKVIQRYYPDLSFEENSKIASYLWKHRFGCFDKYYEYLQIEESKFTSLVTSKIDQLAFKAVPTEKTTSQCTFRFSSSPKRRRTVNLNILKPLCKRVYSQQFHSPLTGITTQDIFVAEENNV +>460519_cbs-7435_MATkp_MATa|MATa1|a1 +MSGNTFREITAACLLSMILSTDDYTKPATSCHMHQLTAPDLSDYSLLDALFVTCDRALEYQALTYRLQASMEDAMKIIESARYQTKDLPTLDNIFKTFYKTLLDTIESIEATGTSTLLSDFTHQISFFNIDPSVISLSDETKECAYSFFSDSLHHHPVKRTRKTISKEVKKILEEAFKAKAAPNTKERLLIAQRCALTPLQVRMWFTNKRTRNKIKQRLQAQSHP +>460519_cbs-7435_MATkp_MATalpha|MATalpha1|alpha1 +MLPPQPKSFKGIQKPFKCSQYAHSTLDNGNMLQHTKHPELVTYQLETPRTRSLQQIESHLLQETTLGIKRTGENQYSEKRTKSSRSYMSKRINAFIAFRSYYSRQVNDYNKQISISRLLSVAWKNENKELWRSYALLYNSSNSNIPFVDWLQTKGPKNSKRTSTVTKSFISWNELLSTRNKIEDVFLDLQ +>460519_cbs-7435_MATkp_MATalpha|MATalpha2|alpha2 +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRAIALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLSKTQIRNWISNKRRKERSSTVSTELLQALNKSPS +>460519_gs115_MATkp_MATalpha|MATalpha2|alpha2 +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRAIALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLSKTQIRNWISNKRRKERSSTVSTELLQALNKSPS +>460523_cbs-4732_MATog_MATa-MATalpha|MATalpha2|alpha2 +MDDMLTKIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKLCKSSMEGFTTERSLKYTRDCPQYAAVTTSQPRYRIEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTRCSPNDVV +>460523_cbs-4732_MATog_MATa-MATalpha|MATalpha1|alpha1 +MMFHVLCFFGNRTSRVANLCLQLKFPPHNDSPKMALDELNLSFLTHLPQIPTTSPLLKRLLTEYGAIDNGKDSLNKVAPKKSSRHRVQKEKKRLNGFMAFRSFYSRNIANYNSQKALSQDLADAWNREKHQQIWSLYAIQYNHSKSKEPFSRWLEMKLSNKLGKFNSDDPTTRREINVMSHIVVEDVFQECSSQY +>460523_cbs-4732_MATog_MATa-MATalpha|MATa1|a1 +MQFTILNEPSLDSQRREGDLASENYVFGDIRKEGVRILEDSLRSERNALLAIGAGGAADRVKQHYRKLLDDWEAAIMEIRSNDKYESLCCGFAIEKAEVETEMRNTEELDVYKECLVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>4909_cbs-573_MATpk_MATa|MATa1|a1 +MYEMSLGKDVGTIILPILTICSLTSEFCDKNETRKPLNIELPRLESMSGIPTTENSNLRRLIDDCQDSFEKEVGILMNNDLPLTLVEDLFKRYKEFFWEKYKLMNNLIDTPRLPEHLTFDDHWILDDSLSLENLNDIPMLQPYQLSDFEITQAEITTTSDILGEPLTIELSGSQLSEQNTPIFSQNFGIYDYLNSITNEIESSTTDSGHNRRHRPSKDVKEKLEQIFTKVKNPNSVEREYIAKKCNMTPTQVRIWFTNKRARQIKTRDT +>4909_cbs-573_MATpk_MATa|MATa2|a2 +MAKLTDSGSTRNRSESVSQNVGAVEFVQVVKQEHGYDEKEYKKILRSEIEKESKKPRNIFLIYRSLVKQYLMNYLSIKGFTEISEESGKLWKSRSSEVEHYVKYLAEQEERFFENEQTQRLKDRSSSASEKLKEKTLSQLTVDGYNGREPKGETVKKDVFRVYKPKKRKRSGVRSKMKSLRSKQNRVEDIFCVPNIEFQDIK +>4909_cbs-573_MATpk_MATalpha|MATalpha1|alpha1 +MARMWNKSSTLRVKKQRPSRICKTTTKATIFIAKPTPRYVIPLEVEELLGKYISRNNLDLRTLSNVKKKNKKHKTVVNGFTAFRTYYSKFGKTYEDQENLSKELANLWKQTPSIQSTWRGYSEEYRASETDLPFVEWFDTYKSLIKPEPEKRDYITQTTTMSHLTVEDVYDNLGSNSMN +>4909_cbs-573_MATpk_MATalpha|MATalpha2|alpha2 +MQHDELLEVYIHLKDVLRQLAETPIDEELSSTDLLKDMLNIKEILESGSPLQKQSCELIRMIRRISTSIYDMISQRSTLKTKYGLDLKSWDLDNSMMNTEDTNLTKNMDIHNSSSIDIDIDDEEISTALRDEGIHSSFCNTDSNSIKTKDGSSRNGKRLPKETIAVLNKWFNDNIENPYVQKEDISYLKAKTNLQASQIKNWVSNKRRKRKGSKVSPSIVEILN +>28985_nrrl-y-1140_MATsc_HMLalpha|MATALPHA1|alpha1 +MKSNAPTFKVAVSKRSCSSVRKTSKKIRSGMIRKPSVSSRYRKHEGVNLYMSKVTPTSIPAPPQVLVAYIKEKVKTLNKSEVLMSLGNSNQLSSRDVKTKTKKKQINDFIAFRSYYSRLLNGILTQTELSTIISKHWTVDKQTRKNWELIAQEYNCDASGKHFFNWLEVNYGIDKQWLYEIVQYEECLTPTTKKPYVENIYNSGFKQRIDSPNQKDITFSQSDNSADWLVDPIFFDTEFLATSFDRTNLIISDCYSLMNSV +>28985_nrrl-y-1140_MATsc_HMLalpha|MATALPHA2|alpha2 +MSRIPIHSLLNPSESCKSISNVPSNYRDLSTFNKERAKVITTFQEMFYSMLENNDDYNKIESLIRNFQPKLTWSHKCESLTFKQKAYLTAIIQKSIKSLLVLLKEKGKMREIEFSRKEVRKINKYRQSSKNFESVNIKILTQDLMHSNNNEFKKGKRFPKSHIQLLENWYSMNRRNPYLAENDLAYISKNTTLTKTQIKNWLANRRRKDKITEVSSDIRNILN +>28985_nrrl-y-1140_MATsc_MATa|MATA1|a1 +MCDNDMADIQSKLSSFCEEIRALALKEGYNLEGDKSPSSKPYFMSWPKEIDVNHPNFAFFTKLQFQYDKSLETILNSCYLQELQLDPTRIIETLQQHFNNSILRYADIENISDVKDESPMSFDTEHECTDTSEDISDKSEISSTNSDNPIQNCYPTYKRSFIKHESRGILEKIFKVKQCPNTSERLYIAQKLDLTPSQVRIWFTNKRMRAKKHTTGKGTKRKSKKYPS +>28985_nrrl-y-1140_MATsc_MATa|MATA2|a2 +MANSTLRRTTFFKLTTTEDEDTIPKLLQPNNNSVAFPNLKRTGKAFTNDTIKENTKKYTRPRNQFVLMRTLFNRRVNNHILQYYNKSKLEKKMFTLTSKITSELWNESSPDLKSYFSLLATLEENWHKYTHYCSWDRNSAQTLSMEPIELSQVRPRLISSLTVGAGSSVSTYTLRELLLVRKIKSRKRKTTTLTQDSSPTTKFKYKFKKQPKTKMNSNLSKLRFKSKQPPTPPEENSNVFKKRYTSENRIIEDLFLM +>381046_cbs-6340_MATsc_HMLalpha|MATALPHA1|alpha1 +MNSTKPSFKVLIKKKGKTLNRRLKNSSKSYRENGANLYMSYSKPQAIPKPPNTLINLVRSKKTSQSSRKCLKNDYILKELDVKKLSIYSNNTVLKKKINPFIGFRSYYAKFAKGRVRQQELSKILSEYWTKNSKIHSVWEFFTQHYNREVTSMCFTLWLEENYQQSYEESELDSECMAKRSQPYIEDLYSGTGEFLTKLTSRELLDISGNHSGFQGSLNSLPFFEEHNRTTDWMLSSILQCDQVPYYPN +>381046_cbs-6340_MATsc_HMLalpha|MATALPHA2|alpha2 +MSHISVSSLLNLPVFQASHQEKATSPITSQSCLVASGKQRKFNATAKKRLERWFCSNIQYPYPNKIQILSLSKNTNLSIKQIQNWMSNRRRKSRKESIDSLLEPILNS +>4932_s288c_MATsc_HMRa|MATA2|a2 +MRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>4932_s288c_MATsc_HMRa|MATA1|a1 +MDDICSMAENINRTLFNILGTEIDEINLNTNNLYNFIMESNLTKVEQHTLHKNISNNRLEIYHHIKKEKSPKGKSSISPQARAFLEQVFRRKQSLNSKEKEEVAKKCGITPLQVRVWFINKRMRSK +>4932_s288c_MATsc_MATalpha|MATALPHA2|alpha2 +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>4932_s288c_MATsc_MATalpha|MATALPHA1|alpha1 +MFTSKPAFKIKNKASKSYRNTAVSKKLKEKRLAEHVRPSCFNIIRPLKKDIQIPVPSSRFLNKIQIHRIASGSQNTQFRQFNKTSIKSSKKYLNSFMAFRAYYSQFGSGVKQNVLSSLLAEEWHADKMQHGIWDYFAQQYNFINPGFGFVEWLTNNYAEVRGDGYWEDVFVHLAL +>4959_cbs767_MTL_A|MTLalpha1|alpha1 +MTSEVAKVIPKRFTSRFTVSKTGKRSNSNIKNEAIDIGRQRKVKSMQVVFFTELPSLPTPSIDLQEILIRFNQATRNCAGDSSWGSQLFDMKVPTRKSRIRCKKTRCLNGFMAFRSFYSRSIYNVEHQRELSTLLGKLWKNEPNKAIWNRYAIEYNSQAINQDFVDWLFKALGLEVETFPIPYTSTVRNESWTFSSRNNENAVEDIYYIGTV +>4959_cbs767_MTL_A|MTLA1|a1 +MEGYTKSHDVVVEVPENENIKAYTTRVESGHISEPSSSNGSNLTLNVKNEYENISSDGKVIDRYQLLKEKGYSHGFLLEEASISVTCETPDPHLQQQRKVSRFGREVHENTNVFPNKKGSYKCDSEASNRFLENVFKTNSYPTSKERHIIAKKCGLSPTQVGAWFTNRRNVSE +>4959_cbs767_MTL_A|MTLA2|a2 +MSLSPDKIFTFCFKLPRRIDIPVESNQPTTKKNFKIKELTTTNSYPSISINIKQYVSFTSEKYIEASRKPTAKDKKTAITRGSFRTRNKFIIARNLLCRLVKHEKALSSTEISKVVSLVWKESNKSLHDYFEYLSLLETYWYDKLMSSYGNSTLQATATAAVSIRLSSVPYENLCASCYNKHKQKKTLNYDYSKSAKFRVHKDFKEKKKTFLTIKDHSKKLEKLSDAFRINQCTITTNFGIVTEDMFFNSL +>36911_cl143_MTL_A|MTLA2|a2 +MTAEEPLYSRTFSLPSKRKFNKQPTNAKRFPLRATNLISKAPQVDEEFLKRYRSRNQFIMARQLISPFLKGVHNSASRSKLVSCIWKTKTSQFEKYFRYLAALDSNYFEELIKFRSANNAFVGDSYRSMCSSSYSLKRTYRDAKFKVHKFKTEIKRSFIVKQNEYFFGLQVEDIMG +>36911_cl143_MTL_A|MTLA1|a1 +MILSDGKNDLQHFLTEVRALTSAYVTDELPDHNRIESQSFIERMGHLVNNIFMKINVFTILKSLQMSLIYEHKMRKAEVKNLDQKFYLRRTRVKFFKREASKAVRKSFTSEAKTKLELFYLANTNPRKAEIVKMARECNMPEEKVRAWFANKRYRERTQNKCIQ +>42374_d1558_MTL_alpha|MTLalpha2|alpha2 +MNTHSEALFQINQRLLFHIRTLSNFPVFDPQKITSDVETDIKKMVSIMKFKRLTNEDEAIMKMINDCSKVLQEMLLERIMLQQDSIQFFTEKEASDSSFSNTVDDAIEDGDDRKTKSRRLTKKQLLVLEGWFQKHKNHPYSQKDQTNLLIKSTKLSKSQVQNWISNRRRKEKNTKVSPELAALLLTG +>42374_d1558_MTL_alpha|MTLalpha1|alpha1 +MGNKKRSMKTVPKEFTSLFRVHPGRDPPRRDTREVQKSKKHGFRFTSLPDLPVASNALQELLLEYGLLNDIRWDSKGSKASKTKKIKLKPINSFIAFRSFYSRTISNPEHQRELSSKLAEVWTQESNQEVWKQYTQSYNNYLLLPEAKLNFVDWLCEALDYTIENATPSLEDISLTSYNQLLSGTIEDVYMLK +>4929_nrrl-y-2075_MTL_alpha|MTLalpha1|alpha1 +MWYPTTSQNTLPLEIIRSMGINKHSVPSKYATSFSARQKRPSKSVRSTTKHVRKQEIVAIPLLPPLPQPSEHLKSVLLTINNMDHSNMSATASRPSFSGKGRKVQCAKINGFFAFRSFYAKSIKNIHYQRILSSLLSAAWKAEESKETWRRYAIIYNESRRDSDFVQWLCKSLNLYESGGDKVEINDGIIQTGNYNSMDIHVEDVYLVPK +>498019_b11205_MTL_A|MTLA1|a1 +MDQKPSKFNELFQTVSTLEQLTSFSKKKWKIPLAQDNYVDSMKTLTEEAGFGKSFHKAIEQVYLWKKVTKNFENEESSLTLTSLCEATSVTKNENASCKRRRSHLTMGIKEYLEQVFKKKPHPTRSEYEVIARSCNLSSRQVRVWFTNKRYRSKPRGNLKEEDEDR +>498019_b11221_MTL_alpha|MTLalpha1|alpha1 +MVSNEDIHLKSKEANFLMIRTGLSIPALPEPSETIINSLAECDASMDDIDLFKPSNRSKRRKAVKRRRLNGFIAFSSYYSRAAKSHSSQRKLSSLLAKVWKEEANKHVWSTYASVYNETGGNDDFILWLEKNLEQNEKDSTRSFTQKRKHHAFINDIEDVFLTSQANKNLAN +>498019_b11243_MTL_A|MTLA2|a2 +MIYSTTSRLPAKSKGPLSKRTRCGHFPYSELKFLHTSTEATDKSKRYRPRNQFMMARQHLTSTFKDGRCARVKSIRASQVCSYYCYLAGLAAAYYETSSAQNSTASFQCNTARLHHVNSAFKVVKFRVQLKQRVPRKEKSFFGLQVEDVVPS +>498019_b11243_MTL_A|MTLA1|a1 +MDQKPSNFNELFQTVSTLERLTSFSKKKWKIPLAQDNYVDSMKRLTEEAGFGKSFHKAIEQVYLWKKVTKNFENEESSLTLTSLCEATSVTKNENASCKRRRSHLTMGIKEYLEQVFKKKPHPTRSEYEVIAKSCNLSSRQVRVWFTNKRYRSKPRGNLKEEDEDR +>5476_sc5314_MTL_A|MTLA2|a2 +MPYTFNFPKSQSSFKYSIKSLTNSRSFPMISIYVTPQSTPKFINNQSIDKMTVLSSFRSRNSFIIARSILSKLLRKNKADFKKVSKSVSLLWGSVDNSFKNYFEYLSILESQWHDKKSSNLNPISSTRTTSELNYIELCSSIYNQSRKHPRSVQGNKAKFRLYKNVSKKSNRKPRRLKNGFKVSRSTINCSYGIYTEDVFQ +>5476_sc5314_MTL_A|MTLA1|a1 +MNSEIESSLTLLKSVEKLVQATSVYKNEDNEEIFLQLKRERQENSNSHEETETFFHESLDRLMKLSSIAGSKVQYELSLNNLYNLLLRTRKQEEEEAFIFPTVSTEEFALEQITFEVSDQMDDDKDSEDDILIKGEEIKKSKKKRQRLDNSTKEFLEKVFEKNKQPNRRERELIAEKHGVSLSQIRVWFTNKRMRKKEPKSKAKSSSNST +>5476_sc5314_MTL_alpha|MTLalpha2|alpha2 +MNSHSEALFEINQRLSSHIRTMSNFPVFDPQKITNDVEANIKRMTSILKSKRLTREDEDLMKMINDCSKVLHEMLLERIMLQQDSIQFFTEKEASDSPFSNSADTIDEDDDKKIKSRRLTKKQLLVLEGWFQKHKNHPYSQKDQTNLLIKSTKLSKSQVQNWISNRRRKEKNTKVSPELAALLLTG +>5476_sc5314_MTL_alpha|MTLalpha1|alpha1 +MGNKKKTRKTVPKEFISLFRVHSGRDAPRRDTREVQKSKKHGFRFTSLPDLPVASNALQELLLEYGLLNDIKWDSKGLKASKNKKTKLKPINSFIAFRSFYSRTISNPEHQRELSSKLADVWTQESNQEVWKQYTQSYNNYLLLPDAKLNFVDWLCEALDYTIDNTTPQIEDISLTSYNQLLSGTIEDVYIMK diff --git a/results/2026-10-30_mat_hmms/data/loco.tsv b/results/2026-10-30_mat_hmms/data/loco.tsv new file mode 100644 index 00000000..0117fd1d --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/loco.tsv @@ -0,0 +1,109 @@ +scheme heldout id true species a1_score alpha2_score call n_a1_train n_alpha2_train +clade Dipodascales 4952_w29_MATyl_A|mata1 a1 Yarrowia lipolytica 46.4 0.0 a1 28 25 +clade Pichiaceae XP_020074339.1|Mtla1p a1 Hyphopichia burtonii 19.1 115.4 alpha2 23 19 +clade Pichiaceae 460519_cbs-7435_MATkp_MATa|MATa1 a1 Komagataella phaffii 97.9 16.8 a1 23 19 +clade Pichiaceae CAE84419.1|MATa1_transcription_factor a1 Ogataea angusta 104.4 21.7 a1 23 19 +clade Pichiaceae 1005962_dl-1_MATog_MATa-MATalpha|MATa1 a1 Ogataea parapolymorpha 97.0 17.5 a1 23 19 +clade Pichiaceae 460523_cbs-4732_MATog_MATa-MATalpha|MATa1 a1 Ogataea polymorpha 104.4 21.7 a1 23 19 +clade Pichiaceae 4909_cbs-573_MATpk_MATa|MATa1 a1 Pichia kudriavzevii 87.1 21.6 a1 23 19 +clade Pichiaceae 460519_cbs-7435_MATkp_MATalpha|MATalpha2 alpha2 Komagataella phaffii 16.4 102.9 alpha2 23 19 +clade Pichiaceae CAE84417.1|MATalpha2_transcription_factor alpha2 Ogataea angusta 25.5 100.8 alpha2 23 19 +clade Pichiaceae 1005962_dl-1_MATog_MATa-MATalpha|MATalpha2 alpha2 Ogataea parapolymorpha 26.6 104.3 alpha2 23 19 +clade Pichiaceae 460523_cbs-4732_MATog_MATa-MATalpha|MATalpha2 alpha2 Ogataea polymorpha 25.5 100.8 alpha2 23 19 +clade Pichiaceae QFP92360.1|mating-type_protein_ALPHA2 alpha2 Ogataea thermomethanolica 0.0 0.0 alpha2 23 19 +clade Pichiaceae 4909_cbs-573_MATpk_MATalpha|MATalpha2 alpha2 Pichia kudriavzevii 27.2 105.6 alpha2 23 19 +clade Saccharomycetaceae 28985_nrrl-y-1140_MATsc_MATa|MATA1 a1 Kluyveromyces lactis 88.3 12.4 a1 19 8 +clade Saccharomycetaceae XP_022675170.1|mating-type_protein_A1 a1 Kluyveromyces marxianus 84.0 11.5 a1 19 8 +clade Saccharomycetaceae KAM3161348.1|Mating_type_a1_protein a1 Lachancea thermotolerans 54.6 0.0 a1 19 8 +clade Saccharomycetaceae CAO02580.1|mating_type_a1_protein a1 Lachancea waltii 85.9 14.2 a1 19 8 +clade Saccharomycetaceae CAL9736377.1|mating-type_protein_A1 a1 Monosporozyma servazzii 60.0 0.0 a1 19 8 +clade Saccharomycetaceae CAL9731627.1|mating-type_protein_A1 a1 Monosporozyma unispora 77.3 20.1 a1 19 8 +clade Saccharomycetaceae CAE84400.1|MATa1_protein a1 Nakaseomyces delphensis 74.8 24.7 a1 19 8 +clade Saccharomycetaceae 4932_s288c_MATsc_HMRa|MATA1 a1 Saccharomyces cerevisiae 72.4 0.0 a1 19 8 +clade Saccharomycetaceae BAX02937.1|a1_transcriptional_factor a1 Zygosaccharomyces rouxii 74.4 14.1 a1 19 8 +clade Saccharomycetaceae BBB04551.1|mating_type_a1 a1 Zygosaccharomyces sp. 74.0 13.0 a1 19 8 +clade Saccharomycetaceae XP_003956854.1|homeodomain_mating_type_protein_alpha2_K alpha2 Kazachstania africana 34.9 102.8 alpha2 19 8 +clade Saccharomycetaceae 28985_nrrl-y-1140_MATsc_HMLalpha|MATALPHA2 alpha2 Kluyveromyces lactis 29.9 84.7 alpha2 19 8 +clade Saccharomycetaceae CCA89264.1|mating-type_alpha2_protein alpha2 Kluyveromyces marxianus 25.7 89.8 alpha2 19 8 +clade Saccharomycetaceae 381046_cbs-6340_MATsc_HMLalpha|MATALPHA2 alpha2 Lachancea thermotolerans 34.2 65.9 alpha2 19 8 +clade Saccharomycetaceae CAO02574.1|mating_type_alpha2_protein alpha2 Lachancea waltii 43.0 77.3 alpha2 19 8 +clade Saccharomycetaceae CAL1760704.1|mating-type_protein_Alpha2p alpha2 Maudiozyma bulderi 23.7 93.7 alpha2 19 8 +clade Saccharomycetaceae SMN20089.1|MATALPHA2 alpha2 Maudiozyma saulgeensis 22.3 90.8 alpha2 19 8 +clade Saccharomycetaceae XP_070910655.1|homeodomain_mating_type_protein_alpha2 alpha2 Nakaseomyces bracarensis 26.2 73.1 alpha2 19 8 +clade Saccharomycetaceae AAO25604.1|ALPHA2 alpha2 Nakaseomyces delphensis 25.9 76.4 alpha2 19 8 +clade Saccharomycetaceae QHS64598.1|MTLalpha2 alpha2 Nakaseomyces glabratus 32.2 81.9 alpha2 19 8 +clade Saccharomycetaceae 4932_s288c_MATsc_MATalpha|MATALPHA2 alpha2 Saccharomyces cerevisiae 23.4 99.9 alpha2 19 8 +clade Saccharomycetaceae XP_018223412.1|homeodomain_mating_type_protein_alpha2_D alpha2 Saccharomyces eubayanus 23.4 99.9 alpha2 19 8 +clade Saccharomycetaceae XP_056080738.1|homeodomain_mating_type_protein_alpha2_S alpha2 Saccharomyces mikatae 23.4 99.9 alpha2 19 8 +clade Saccharomycetaceae QHS71949.1|Matalpha2 alpha2 Saccharomyces paradoxus 23.4 99.9 alpha2 19 8 +clade Saccharomycetaceae XP_003685884.1|homeodomain_mating_type_protein_alpha2_T alpha2 Tetrapisispora phaffii 26.6 103.9 alpha2 19 8 +clade Saccharomycetaceae BAX02933.1|alpha2_transcriptional_factor alpha2 Zygosaccharomyces rouxii 23.1 107.4 alpha2 19 8 +clade Saccharomycetaceae BBB04552.1|mating_type_alpha2 alpha2 Zygosaccharomyces sp. 23.1 107.4 alpha2 19 8 +clade Serinales 5476_sc5314_MTL_A|MTLA1 a1 Candida albicans 89.1 0.0 a1 17 23 +clade Serinales CAK7898777.1|MATa1_protein a1 Candida anglica 75.9 18.1 a1 17 23 +clade Serinales XP_002546761.1|Mtla1p a1 Candida tropicalis 67.9 0.0 a1 17 23 +clade Serinales 498019_b11205_MTL_A|MTLA1 a1 Candidozyma auris 84.4 19.1 a1 17 23 +clade Serinales 36911_cl143_MTL_A|MTLA1 a1 Clavispora lusitaniae 41.9 14.8 a1 17 23 +clade Serinales 4959_cbs767_MTL_A|MTLA1 a1 Debaryomyces hansenii 70.9 16.7 a1 17 23 +clade Serinales XP_003870289.1|Mtla1p a1 Lodderomyces orthopsilosis 82.3 0.0 a1 17 23 +clade Serinales XP_051608499.1|Mtla1p a1 Lodderomyces theae 67.7 0.0 a1 17 23 +clade Serinales XP_062812646.1|Mtla1p a1 Priceomyces carsonii 94.2 17.6 a1 17 23 +clade Serinales XP_064741610.1|Mtla1p a1 Scheffersomyces amazonensis 83.6 19.1 a1 17 23 +clade Serinales XP_064762305.1|Mtla1p a1 Scheffersomyces coipomensis 62.2 27.0 a1 17 23 +clade Serinales XP_001386328.2|Mtla1p a1 Scheffersomyces stipitis 54.5 0.0 a1 17 23 +clade Serinales 5476_sc5314_MTL_alpha|MTLalpha2 alpha2 Candida albicans 36.6 114.1 alpha2 17 23 +clade Serinales 42374_d1558_MTL_alpha|MTLalpha2 alpha2 Candida dubliniensis 39.4 114.0 alpha2 17 23 +species Candida albicans 5476_sc5314_MTL_A|MTLA1 a1 Candida albicans 112.5 14.4 a1 28 24 +species Candida albicans 5476_sc5314_MTL_alpha|MTLalpha2 alpha2 Candida albicans 35.6 165.8 alpha2 28 24 +species Candida anglica CAK7898777.1|MATa1_protein a1 Candida anglica 94.4 17.6 a1 28 25 +species Candida dubliniensis 42374_d1558_MTL_alpha|MTLalpha2 alpha2 Candida dubliniensis 33.3 165.1 alpha2 29 24 +species Candida tropicalis XP_002546761.1|Mtla1p a1 Candida tropicalis 96.3 0.0 a1 28 25 +species Candidozyma auris 498019_b11205_MTL_A|MTLA1 a1 Candidozyma auris 92.3 20.9 a1 28 25 +species Clavispora lusitaniae 36911_cl143_MTL_A|MTLA1 a1 Clavispora lusitaniae 41.4 14.8 a1 28 25 +species Debaryomyces hansenii 4959_cbs767_MTL_A|MTLA1 a1 Debaryomyces hansenii 80.1 16.2 a1 28 25 +species Hyphopichia burtonii XP_020074339.1|Mtla1p a1 Hyphopichia burtonii 19.6 123.2 alpha2 28 25 +species Kazachstania africana XP_003956854.1|homeodomain_mating_type_protein_alpha2_K alpha2 Kazachstania africana 39.4 194.2 alpha2 29 24 +species Kluyveromyces lactis 28985_nrrl-y-1140_MATsc_MATa|MATA1 a1 Kluyveromyces lactis 104.7 0.0 a1 28 24 +species Kluyveromyces lactis 28985_nrrl-y-1140_MATsc_HMLalpha|MATALPHA2 alpha2 Kluyveromyces lactis 24.3 121.8 alpha2 28 24 +species Kluyveromyces marxianus XP_022675170.1|mating-type_protein_A1 a1 Kluyveromyces marxianus 101.0 20.2 a1 28 24 +species Kluyveromyces marxianus CCA89264.1|mating-type_alpha2_protein alpha2 Kluyveromyces marxianus 26.6 117.9 alpha2 28 24 +species Komagataella phaffii 460519_cbs-7435_MATkp_MATa|MATa1 a1 Komagataella phaffii 97.9 14.0 a1 28 24 +species Komagataella phaffii 460519_cbs-7435_MATkp_MATalpha|MATalpha2 alpha2 Komagataella phaffii 29.0 106.2 alpha2 28 24 +species Lachancea thermotolerans KAM3161348.1|Mating_type_a1_protein a1 Lachancea thermotolerans 64.5 0.0 a1 28 24 +species Lachancea thermotolerans 381046_cbs-6340_MATsc_HMLalpha|MATALPHA2 alpha2 Lachancea thermotolerans 29.3 77.8 alpha2 28 24 +species Lachancea waltii CAO02580.1|mating_type_a1_protein a1 Lachancea waltii 96.9 18.5 a1 28 24 +species Lachancea waltii CAO02574.1|mating_type_alpha2_protein alpha2 Lachancea waltii 35.7 87.8 alpha2 28 24 +species Lodderomyces orthopsilosis XP_003870289.1|Mtla1p a1 Lodderomyces orthopsilosis 141.7 0.0 a1 28 25 +species Lodderomyces theae XP_051608499.1|Mtla1p a1 Lodderomyces theae 91.3 0.0 a1 28 25 +species Maudiozyma bulderi CAL1760704.1|mating-type_protein_Alpha2p alpha2 Maudiozyma bulderi 20.6 216.2 alpha2 29 24 +species Maudiozyma saulgeensis SMN20089.1|MATALPHA2 alpha2 Maudiozyma saulgeensis 18.6 211.2 alpha2 29 24 +species Monosporozyma servazzii CAL9736377.1|mating-type_protein_A1 a1 Monosporozyma servazzii 81.4 0.0 a1 28 25 +species Monosporozyma unispora CAL9731627.1|mating-type_protein_A1 a1 Monosporozyma unispora 106.3 18.0 a1 28 25 +species Nakaseomyces bracarensis XP_070910655.1|homeodomain_mating_type_protein_alpha2 alpha2 Nakaseomyces bracarensis 30.7 164.8 alpha2 29 24 +species Nakaseomyces delphensis CAE84400.1|MATa1_protein a1 Nakaseomyces delphensis 84.3 22.6 a1 28 24 +species Nakaseomyces delphensis AAO25604.1|ALPHA2 alpha2 Nakaseomyces delphensis 30.0 169.5 alpha2 28 24 +species Nakaseomyces glabratus QHS64598.1|MTLalpha2 alpha2 Nakaseomyces glabratus 32.1 158.4 alpha2 29 24 +species Ogataea angusta CAE84419.1|MATa1_transcription_factor a1 Ogataea angusta 148.8 17.2 a1 28 24 +species Ogataea angusta CAE84417.1|MATalpha2_transcription_factor alpha2 Ogataea angusta 28.4 154.8 alpha2 28 24 +species Ogataea parapolymorpha 1005962_dl-1_MATog_MATa-MATalpha|MATa1 a1 Ogataea parapolymorpha 132.1 14.1 a1 28 24 +species Ogataea parapolymorpha 1005962_dl-1_MATog_MATa-MATalpha|MATalpha2 alpha2 Ogataea parapolymorpha 31.5 153.8 alpha2 28 24 +species Ogataea polymorpha 460523_cbs-4732_MATog_MATa-MATalpha|MATa1 a1 Ogataea polymorpha 148.8 17.2 a1 28 24 +species Ogataea polymorpha 460523_cbs-4732_MATog_MATa-MATalpha|MATalpha2 alpha2 Ogataea polymorpha 28.4 154.8 alpha2 28 24 +species Ogataea thermomethanolica QFP92360.1|mating-type_protein_ALPHA2 alpha2 Ogataea thermomethanolica 0.0 15.7 alpha2 29 24 +species Pichia kudriavzevii 4909_cbs-573_MATpk_MATa|MATa1 a1 Pichia kudriavzevii 87.4 21.6 a1 28 24 +species Pichia kudriavzevii 4909_cbs-573_MATpk_MATalpha|MATalpha2 alpha2 Pichia kudriavzevii 30.6 111.8 alpha2 28 24 +species Priceomyces carsonii XP_062812646.1|Mtla1p a1 Priceomyces carsonii 112.5 17.0 a1 28 25 +species Saccharomyces cerevisiae 4932_s288c_MATsc_HMRa|MATA1 a1 Saccharomyces cerevisiae 91.8 0.0 a1 28 24 +species Saccharomyces cerevisiae 4932_s288c_MATsc_MATalpha|MATALPHA2 alpha2 Saccharomyces cerevisiae 27.7 260.0 alpha2 28 24 +species Saccharomyces eubayanus XP_018223412.1|homeodomain_mating_type_protein_alpha2_D alpha2 Saccharomyces eubayanus 20.0 260.0 alpha2 29 24 +species Saccharomyces mikatae XP_056080738.1|homeodomain_mating_type_protein_alpha2_S alpha2 Saccharomyces mikatae 20.0 260.0 alpha2 29 24 +species Saccharomyces paradoxus QHS71949.1|Matalpha2 alpha2 Saccharomyces paradoxus 20.0 260.0 alpha2 29 24 +species Scheffersomyces amazonensis XP_064741610.1|Mtla1p a1 Scheffersomyces amazonensis 142.1 21.6 a1 28 25 +species Scheffersomyces coipomensis XP_064762305.1|Mtla1p a1 Scheffersomyces coipomensis 102.6 27.5 a1 28 25 +species Scheffersomyces stipitis XP_001386328.2|Mtla1p a1 Scheffersomyces stipitis 73.3 0.0 a1 28 25 +species Tetrapisispora phaffii XP_003685884.1|homeodomain_mating_type_protein_alpha2_T alpha2 Tetrapisispora phaffii 25.4 197.6 alpha2 29 24 +species Yarrowia lipolytica 4952_w29_MATyl_A|mata1 a1 Yarrowia lipolytica 46.4 0.0 a1 28 25 +species Zygosaccharomyces rouxii BAX02937.1|a1_transcriptional_factor a1 Zygosaccharomyces rouxii 98.3 18.3 a1 28 24 +species Zygosaccharomyces rouxii BAX02933.1|alpha2_transcriptional_factor alpha2 Zygosaccharomyces rouxii 19.2 232.3 alpha2 28 24 +species Zygosaccharomyces sp. BBB04551.1|mating_type_a1 a1 Zygosaccharomyces sp. 110.4 18.4 a1 28 24 +species Zygosaccharomyces sp. BBB04552.1|mating_type_alpha2 alpha2 Zygosaccharomyces sp. 23.8 232.3 alpha2 28 24 diff --git a/results/2026-10-30_mat_hmms/data/pfam_hits.tsv b/results/2026-10-30_mat_hmms/data/pfam_hits.tsv new file mode 100644 index 00000000..0b4070ea --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/pfam_hits.tsv @@ -0,0 +1,23 @@ +Pkud_MATa2_control HMG_box 18.4/25.4 +PF_JOY008_MATa2 HMG_box 13.5/25.4 +PF_JOY008_MATa1 Homeobox_KN 29.5/24.0; Homeodomain 63.8/22.9 +PF_TBRC4498_MATalpha1 MATalpha_HMGbox 36.4/28.1 +BC_MATa2 HMG_box 14.5/25.4 +BC_MATa1 Homeobox_KN 28.6/24.0; Homeodomain 54.2/22.9 +BC_MATalpha1 MATalpha_HMGbox 33.2/28.1 +SS.1982.2.p3 Homeodomain 16.1/22.9 +SS.1982.1.p3 Homeodomain 16.7/22.9 +SS.1982.2.p4 MATalpha_HMGbox 54.2/28.1 +SS.1982.2.p5 Homeobox_KN 34.3/24.0; Homeodomain 56.8/22.9 +SS.2002.2.p2 Homeobox_KN 33.1/24.0; Homeodomain 54.2/22.9 +SS.2001.1.p4 Homeobox_KN 18.4/24.0; Homeodomain 33.9/22.9 +SS.2002.1.p2 Homeobox_KN 17.9/24.0; Homeodomain 33.1/22.9 +BCreg_+0_0 +BCreg_+0_182 +BCreg_+1_229 +BCreg_+2_26 +BCreg_+2_268 +BCreg_-0_79 +BCreg_-1_0 +BCreg_-1_128 +BCreg_-2_227 diff --git a/results/2026-10-30_mat_hmms/data/public_a1.faa b/results/2026-10-30_mat_hmms/data/public_a1.faa new file mode 100644 index 00000000..07721ae1 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/public_a1.faa @@ -0,0 +1,343 @@ +>AAG21094.1 a1p [Kluyveromyces lactis] +MCDNDMADIQSKLSSFCEEIRALALKEGYNLEGDKSPSSKPYFMSWPKEIDVNHPNFAFFTKLQFQYDKS +LETILNSCYLQELQLDPTRIIETLQQHFNNSILRYADIENISDVKDESPMSFDTEHECTDTSEDISDKSE +ISSTNSDNPIQNCYPTYKRSFIKHESRGILEKIFKVKQCPNTSERLYIAQKLDLTPSQVRIWFTNKRMRA +KKHTTGKGTKRKSKKYPS + +>ODV70591.1 homeobox-domain-containing protein [Williopsis jadinii] +MSTKDVSNYQEAIEKLESIVEFSSFFTNSHPKPIYDKVLLNDPFDKSTLMKRAPTSCQDHLNDLINTFEE +SLKRVLDEQILPPETVIGQYITRLNRVIELASQYESITGADPERYGYSITPYSVRTASVKVTLGKRSPTS +SDLEDNGSQSKLRSKKRTPISKEAKQLLETIFETKRTPNGKERKIIAEKCGLTPLQVRVWFTNKRMRSK + +>AOW29605.1 Mtla1p [Candida albicans SC5314] +MNSEIESSLTLLKSVEKLVQATSVYKNEDNEEIFLQLKRERQENSNSHEETETLFHESLDRLMKLSSIAG +SKVQYELSLNNLYNLLLRTRKQEEEEAFIFPTVSTEEFALEQITFEVSDQMDDDKDSEDDILIKGEEIKK +SKKKRQRLDNSTKEFLEKVFEKNKQPNRRERELIAEKHGVSLSQIRVWFTNKRMRKKEPKSKAKSSSNST + +>XP_019330959.1 Mtla1p [Candida albicans SC5314] +MNSEIESSLTLLKSVEKLVQATSVYKNEDNEEIFLQLKRERQENSNSHEETETLFHESLDRLMKLSSIAG +SKVQYELSLNNLYNLLLRTRKQEEEEAFIFPTVSTEEFALEQITFEVSDQMDDDKDSEDDILIKGEEIKK +SKKKRQRLDNSTKEFLEKVFEKNKQPNRRERELIAEKHGVSLSQIRVWFTNKRMRKKEPKSKAKSSSNST + +>XP_020074339.1 Mtla1p [Hyphopichia burtonii NRRL Y-1933] +MKVDNYQPKQDIESRLCQLNGILTHLKIHITNVCTLPDFQPESMQKDLTGDCLVVRLLLDYNIQLSESEQ +ALLAKINKLSNLLILMIEERQRIRIIFDRTPRTLQAQTVVEEANKISDASDKKISFEVLTQSDISKGPKT +NRLQDHQVLILNEWYGRNKKHPYVDENSLKYLIEKTRLSETQVRNWFSNKRRKHRSNKVSHELEPLLS + +>BAX02937.1 a1 transcriptional factor [Zygosaccharomyces rouxii] +MSDKTFNSPNDLQKQYLNSFHKVFKTISNSSLSTQEKGVLIRCISQNYSSSFRMTISSLANEYCGDQYIF +NKDSIKRSYSERVLGKDCKGKNRAFSKESRKFLESVFEKKKTLTPREREIVGASCGLTPLQVRIWFNNKR +MRSK + +>BAX02940.1 a1 transcriptional factor [Zygosaccharomyces rouxii] +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIF +EGNSSKNSSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWFNNRR +MRSK + +>BAX02946.1 a1 transcriptional factor [Zygosaccharomyces rouxii] +MSDKTFNSPNDLQKQYLNSFHKVFKTISNSSLSTQEKGVLIRCISQNYSSSFRMTISSLANEYCGDQYIF +NKDSIKRSYSERVLGKDCKGKNRAFSKESRKFLESVFEKKKTLTPREREIVGASCGLTPLQVRIWFNNKR +MRSK + +>CAA24622.1 MATa1 [Saccharomyces cerevisiae] +MDDICSMAENINRTLFNILGTEIDEINLNTNNLYNFIMESNLTKVEQHTLHKNISNNRLEIYHHIKKEKS +PKGKSSISPQARAFLEQVFRRKQSLNSKEKEEVAKKCGITPLQVRFINKRMRSK + +>XP_022673679.1 uncharacterized protein KLMA_10142 [Kluyveromyces marxianus DMKU3-1042] +MDGHDKKNPILLLKELCQEAQGLAGVQLKEDGFSSPFKTFSIPFPEEAVKKCSNKALLLQLYHQYSTSLE +TILNKCPLEDLKVDPQMVADAFNNQFLRTIQLLPVENNLKIKEQNPSDSSGKEDSKNDTVEEDSPSPDNQ +NSRTAKKFFLEPEAKDLLEKVFKVKKCPNTSERLFIAQKLGLTASQVRIWFTNKRMRTKSKQKKVKPIAG +PIK + +>XP_022675170.1 mating-type protein A1 [Kluyveromyces marxianus DMKU3-1042] +MDGHDKKNPILLLKELCQEAQGLAGVQLKEDGFSSPFKTFSIPFPEEAVKKCSNKALLLQLYHQYSTSLE +TILNKCPLEDLKVDPQMVADAFNNQFLRTIQLLPVENNLKIKEQNPSDSSGKEDSKNDTVEEDSPSPDNQ +NSRTAKKFFLEPEAKDLLEKVFKVKKCPNTSERLFIAQKLGLTASQVRIWFTNKRMRTKSKQKKVKPIAG +PIK + +>BBB04551.1 mating type a1 [Zygosaccharomyces sp. NBRC 1876] +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIF +EGNSSKNSSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWFNNRR +MRSK + +>pdb|1YRN|A Chain A, PROTEIN (MAT A1 HOMEODOMAIN) +KKEKSPKGKSSISPQARAFLEQVFRRKQSLNSKEKEEVAKKCGITPLQVRVWFINKRMRSK + +>RCK65208.1 Mating-type-like protein A1 [Candida viswanathii] +MNSEMESSLELLKSISKLVDITSKYADESNEELFEELKRDTHDPWKDTETIFNDSLSSLIKLSSVVGREN +QFELSIRNLYNTMIKTHKEEEESLTVAQEGGDDFKLAKITISVSDQIDDNRSSDKDNGTTENEAKKSKKK +RQRLDNSTKEFLERVFEKKSQPNRRERELIAEKYGISLPQIRVWFTNKRMRKKKPDSTKNESGTSKKS + +>BBD18016.1 putative transcription factor [Ogataea minuta] +MMSNSYIERKADLEISNTDDKYATWSSDDSEFSFDFASLSSSLSMPDDEAKPSVSFLETACFGTEDRDDS +GGLKCFGYESEKAVPVKSTRKAVTKGCRNLLESIFKVKPFPNSKERHIIAEKCGITPTQVRVWFTNKRAR +TKSEKRQV + +>BBD18019.1 putative transcription factor [Ogataea minuta] +MENSDLILEPLFTICHMTDIATNDERHLSESKIETESEGDLEEEEFLLYHSRSLPREKNTKLKEFVAIFQ +NSLNVEIQALKEQKRCHQEIVSLYELYENMMSNSYIERKADLEISNTDDKYATWSSDDSEFSFDFASLSS +SLSMPDDEAKPSVSFLETACFGTEDRDDSGGLKCFGYESEKAVPVKSTRKAVTKGCRNLLESIFKVKPFP +NSKERHIIAEKCGITPTQVRVWFTNKRARTKSEKRQV + +>CAO02580.1 mating type a1 protein [Lachancea waltii] +MSQLPSFLPDIFQKLSRMKEISHVKKMDTSSILITLESIKKACGALLEPDVERRQEVCDKITVGQRRTLF +SKDTRLFLESVFEKKKSPNSRERKAIADKCGLTPVQVRVWFTNRRWR + +>XP_001386328.2 Mtla1p [Scheffersomyces stipitis CBS 6054] +MLRDGECFRYLDSSAVLKYSIQNLFDNAVEKASEKKRTFLDHDAQPQEFHLRSVMISITCSTNLSSKVAL +GDKALVRKRRRKIHSPEIRAILEKEFSLDPSPSRAHRKSIAVRCNMSTSEVRIWFANRRNRFKRYQ + +>QGN14066.1 hypothetical protein FIM1_717 [Kluyveromyces marxianus] +MDGHDKKNPILLLKELCQEAQGLAGVQLKEDGFSSPFKTFSIPFPEEAVKKCSNKALLLQLYHQYSTSLE +TILNKCPLEDLKVDPQMVADAFNNQFLRTIQLLPVENNLKIKEQNPSDSSGKEDSKNDTVEEDSPSPDNQ +NSRTAKKFFLEPEAKDLLEKVFKVKKCPNTSERLFIAQKLGLTASQVRIWFTNKRMRTKSKQKKVKPIAG +PIK + +>QGN15038.1 hypothetical protein FIM1_1722 [Kluyveromyces marxianus] +MDGHDKKNPILLLKELCQEAQGLAGVQLKEDGFSSPFKTFSIPFPEEAVKKCSNKALLLQLYHQYSTSLE +TILNKCPLEDLKVDPQMVADAFNNQFLRTIQLLPVENNLKIKEQNPSDSSGKEDSKNDTVEEDSPSPDNQ +NSRTAKKFFLEPEAKDLLEKVFKVKKCPNTSERLFIAQKLGLTASQVRIWFTNKRMRTKSKQKKVKPIAG +PIK + +>CAA42252.1 mating type regulatory protein, silenced copy at HMR locus [Saccharomyces cerevisiae] +MDDICSMAENINRTLFNILGTEIDEINLNTNNLYNFIMESNLTKVEQHTLHKNISNNRLEIYHHIKKEKS +PKGKSSISPQARAFLEQVFRRKQSLNSKEKEEVAKKCGITPLQVRVWFINKRMRSK + +>BDH85270.1 transcription factor [Zygosaccharomyces sp.] +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIF +EGNSSKNSSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWFNNRR +MRSK + +>CAH2450368.1 Mating-type protein a1 [Komagataella phaffii CBS 7435] +MSGNTFREITAACLLSMILSTDDYTKPATSCHMHQLTAPDLSDYSLLDALFVTCDRALEYQALTYRLQAS +MEDAMKIIESARYQTKDLPTLDNIFKTFYKTLLDTIESIEATGTSTLLSDFTHQISFFNIDPSVISLSDE +TKECAYSFFSDSLHHHPVKRTRKTISKEVKKILEEAFKAKAAPNTKERLLIAQRCALTPLQVRMWFTNKR +TRNKIKQRLQAQSHP + +>XP_049260605.1 Mtla1p, partial [Hemisphaericaspora subhashii] +MNSESKNNRSLLDSIDNLEKVTSKYSTEEKESRFNAFKSQPQVSQAAPEALFDNSLSKLINISSAVEPGN +HFECLIKNLYETMLKSQKEENEQQLTLPIGDPAEYCLTKIDISVSNSLDNAVSSDEDMASEDNLKPKQTR +RRLSNSVKDFLEIHEQENEEEAADGDKPRKLE + +>CAI1883226.1 hypothetical protein SEUBUCD650_0C01590 [Saccharomyces eubayanus] +MDDIYSIAENINKALFKILGTEIDEINLSTNNLYKFVLESNLTKEDQRTLQKNISNNRLEIYHGIKKEKN +HKGKSSISPQARAFLEQVFKRKQSLNSKEKEEVAKKCSITPLQVRVWFINKRMRSK + +>CAI1912004.1 hypothetical protein SEUBUCD646_0N01140 [Saccharomyces eubayanus] +MDDIYSIAENINKALFKILGTEIDEINLSTNNLYKFVLESNLTKEDQRTLQKNISNNRLEIYHGIKKEKN +HKGKSSISPQARAFLEQVFKRKQSLNSKEKEEVAKKCSITPLQVRVWFINKRMRSK + +>CAI1916803.1 a1 [Saccharomyces eubayanus] +MDDIYSIAENINKALFKILGTEIDEINLSTNNLYKFVLESNLTKEDQRTLQKNISNNRLEIYHGIKKEKN +HKGKSSISPQARAFLEQVFKRKQSLNSKEKEEVAKKCSITPLQVRVWFINKRMRSK + +>XP_051608499.1 Mtla1p [Lodderomyces theae] +MNSEYYKGKALLISLSALEDITKSTSSTNLSHSSPNLDVDNGETSQTPDKILEDIKSSCGPIDPRLEWGL +SQLPDTLRRIEQFESENRTNIPLQHNSNFFLQRVAISIGGTVRSKHTQETDLCGSWGGLRRSRKRLSSSS +KELLEKIFLVKQSPNRRERELIAGKCGVTPLQIRVWVCTRSNPICQEATLKIKVLT + +>ACS29267.1 a1 [Clavispora lusitaniae] +MILSDGKNDLQHFLTEVRALTSAYVTDELPDHNRIESQSFIERMGHLVNNIFMKINVFTILKSLQMSLIY +EHKMRKAEVKNLDQKFYLRRTRVKFFKREASKAVRKSFTSEAKTKLELFYLANTNPRKAEIVKMARECNM +PEEKVRAWFANKRYRERTQNKCIQ + +>XP_002546761.1 Mtla1p [Candida tropicalis MYA-3404] +MLKAQKEEEEILTVTEEGGDEFKLAKITISVSDQADDFRASDKESLSSEEEGKKLKKKRQRLDNTTKEFL +EKVFERKSQPNRRERELIAEKYGISLSQIRVWVCIIFYYFQFILSELLTNITSKFTNKRMRKKKPDSSKK +ESDKTDDT + +>XP_062812646.1 Mtla1p [Priceomyces carsonii] +MNSELLKCKPLIENLCALESLTRRFEDTKLDDYQCLEEELSCSTDGTLTNIIGNSRIRALNLSKQFGLGD +KFSIINLYDSMRSYVLENTLPQRIIDRSERTSFTLEKVTVKITNQEGGLKAESQGVTGGSTRKRTYINNE +IRRFLEKAFHKKRNPTRSDRAAIAQKCGLTPLQVRVWFTNKRMRSKSK + +>CAK7898777.1 MATa1 protein [[Candida] anglica] +MRRLLILSQSLDMNPNLLHFNIRNLYDTLAKNNIKKPIVHTLNVYGEDCNFLLESVRMSVSTGKPIKKKF +TQEYKTKIDGTPSEPTYLKKKRTVIPQISRIYLENFFKVNPTPDRNERIMISEKTNLTYDQVRIWFNNKR +MRSKGFNFVLHSIKPGSSLRD + +>XP_064741610.1 Mtla1p [Scheffersomyces amazonensis] +MDSELASCSRLLEDLLLLDLLTRDSSESSEQIGVDSSERSTSGNVFEESADRMIKYASQVGNGGGEYSFC +IRNLYDTLKKESLKTSESIATFIDTADDFHLTKAVITVSNKRNDSFDEEQIISIKNNFMNVKRRRCILSR +TTREFLQKAFEAQPFPNRKDRELIAKKCGISNLQVRVWFTNRRMRSKRRN + +>XP_064762305.1 Mtla1p [Scheffersomyces coipomensis] +MYKLSKLVSDSEEYAFCIRNLYDALEKQSIGNKIYITEHDDRSEGFYLSKATISVSNKKTEPKSNYILDS +IRSSYRASRRKRCILTAETKEFLQKAFDAQPFPNRKDRELLAKKCGLTNLQVRVWVSNSL + +>CAL9731627.1 mating-type protein A1 [Monosporozyma unispora] +MQRSFNVSTKLILESDLCLCERKNLLLQMYDNSSGRTHLTKDTRLFLEKVFIQKQWLTKDERQLIARKCG +LSPLQVRIWFINKRYRNK + +>CAL9736377.1 mating-type protein A1 [Monosporozyma servazzii] +MQQLLNVSSKLILESDLCLCERKNLLLQIYDNSSGRTHLTKDTRLFLEKVFIQKQWLTKDERQLIATKCG +LSPLQVRIWFIKCRHKLI + +>XP_460135.2 Mtla1p [Debaryomyces hansenii CBS767] +MEGYTKSHDVVVEVPENENIKAYTTRVESGHISEPSSSNGSNLTLNVKNEYENISSDGKVIDRYQLLKEK +GYSHGFLLEEASISVTCETPDPHLQQQRKVSRFGREVHENTNVFPNKKGSYKCDSEASNRFLENVFKTNS +YPTSKERHIIAKKCGLSPTQVGAWFTNRRNVSE + +>BGD02941.1 transcriptional factor 1 [Zygosaccharomyces sp.] +MSDKTFNSPNDLQKQYLNSFHKVFKTISNSSLSTQEKGVLIRCISQNYSSSFRMAISSLANEYCGDQYIF +NKDSIKRSYSERVLGKDCKGKNRAFSKESRKFLESVFEKKKTLTPREREIVGASCGLTPLQVRIWFNNKR +MRSK + +>KAM3161348.1 Mating type a1 protein [Lachancea thermotolerans] +MDSRGLIISLESIKKVCLMLLEPNYKSRQEVCDKIYENYATLLQDAVVESKEKPRSGQQRRTLLSKDTRL +FLESVFEKKRSPNSRERKAIADKCGLTPIYISFSVFSSSLGHGSQQKTSPTYSPTFLMFFMLWSNFRTFN +TP + +>YCL22008.1 Mating-type protein a1 [Komagataella phaffii] +MSGNTFREITAACLLSMILSTDDYTKPATSCHMHQLTAPDLSDYSLLDALFVTCDRALEYQALTYRLQAS +MEDAMKIIESARYQTKDLPTLDNIFKTFYKTLLDTIESIEATGTSTLLSDFTHQISFFNIDPSVISLSDE +TKECAYSFFSDSLHHHPVKRTRKTISKEVKKILEEAFKAKAAPNTKERLLIAQRCALTPLQVRMWFTNKR +TRNKIKQRLQAQSHP + +>ADY62670.1 MTLa1 [Lodderomyces metapsilosis] +MASKKGNRKLIPKDFVTLFRVCRSRLRSMKVTKNQHQKPAFSVTSLPNIPEPSKALQLLLIDYRILNVFL +WSPKPESVKTKKSKLRPINSFIAFRSFYSRTISRPEYQRELSSKLAEVWKEEPHREVWEQYTLFYNNYLL +DCNNKLAFVDWLLQTLELDFDNSPLNEVSQTEDAFEFELENCPLNDVSQTEDAFKLTSGTIQDVYLTD + +>ADY62677.1 MTLa1 [Lodderomyces orthopsilosis] +MNSEYYEGKALLDSLTVLEELTNTTTLVRSTTEQGNSDTMDNPRQLVQDLKENCNSIDPRLEWGLEQLPS +NLRSIELFEKEDIVPIPLQFNTKFLLRRVSISVGNSMTEKLTLQDTYLCGSWNSLKTPRQRLSLSSKELL +ERIFLVKQFPNRRERELIAKKCGVSPLQIRVWFTNKRMRSKTVRVECGATIMEVPLDEVQKH + +>ADY62683.1 MTLa1 [Lodderomyces orthopsilosis] +MASKKGNKKLIPKDFVTLFRVCRSRSQDLKVTNNLDRKPAFNIKSLPMIPEPSKGLQSLLLDYSLLNVFS +WSSKSLTSKVKKSKSKPINSFIAFRSFYSRAISRPEYQRELSTKLAKLWKEELHRDVWEQYTLFYNNYLL +ESNNQLTFVDWLLQMLNLESDNNTPIDKSHVEDSLKLKSGTIQDIYLTE + +>ADY62691.1 MTLa1 [Lodderomyces orthopsilosis] +MNSEYHKGKALLDSLTVLEELTKTTTLIGPTKEQGNSDTTDNPHQLLQDLKENWNSIDPRVEWGLKQLPS +NLRTIERFEQEDMVSIPLQSNAKFLLRRVSISVRNSTTKKLTLHDTYLCGSWNALKIPRQRLSLASKELL +ERIFLVKQSPNREERGLIAEKCGVSPLQIRVWFTNKRMRSKRQ + +>ADY62699.1 MTLa1 [Lodderomyces orthopsilosis] +MASKKGNKKLIPKDFVTLFRVCRSRSQDLKVTNNLDRKPAFNIKPLPMIPEPSEELQSLLLDYSLLNVFS +WSSKPLTGKIKKSKSKPINSFIAFRSFYSRAISPPEYQRELSTKLAKLWKEESHRDIWEQYTLFYNNYLL +KSNNKLTFVDWLLQMLNLESDNNTPIDKSHVEDSLKLKSGTIQDVYLTE + +>CCA40182.1 Mating-type protein a1 [Komagataella phaffii CBS 7435] +MSGNTFREITAACLLSMILSTDDYTKPATSCHMHQLTAPDLSDYSLLDALFVTCDRALEYQALTYRLQAS +MEDAMKIIESARYQTKDLPTLDNIFKTFYKTLLDTIESIEATGTSTLLSDFTHQISFFNIDPSVISLSDE +TKECAYSFFSDSLHHHPVKRTRKTISKEVKKILEEAFKAKAAPNTKERLLIAQRCALTPLQVRMWFTNKR +TRNKIKQRLQAQSHP + +>CCA89252.1 mating-type a1 protein [Kluyveromyces marxianus] +MDGHDKKNPILLLKELCQEAQGLAGVQLKEDGFSSPFKTFSIPFPEEAVKKCSNKALLLQLYHQYSTSLE +TILNKCPLEDLKVDPQMVADAFNNQFLRTIQLLPVENNLKIKEQNPSDSSGKEDSKNDTVEEDSPSPDNQ +NSRTAKKFFLEPEAKDLLEKVFKVKKCPNTSERLFIAQKLGLTASQVRIWFTNKRMRTKSKQKVKPIAGP +IK + +>sp|P0CY10.1|MATA1_YEASX RecName: Full=Mating-type protein A1; Short=MATa1 protein +MDDICSMAENINRTLFNILGTEIDEINLNTNNLYNFIMESNLTKVEQHTLHKNISNNRLEIYHHIKKEKS +PKGKSSISPQARAFLEQVFRRKQSLNSKEKEEVAKKCGITPLQVRVWFINKRMRSK + +>sp|Q9UW23.2|MATA1_CANAL RecName: Full=Mating-type-like protein A1; Short=MTLa1 protein +MNSEIESSLTLLKSVEKLVQATSVYKNEDNEEIFLQLKRERQENSNSHEETETLFHESLDRLMKLSSIAG +SKVQYELSLNNLYNLLLRTRKQEEEEAFIFPTVSTEEFALEQITFEVSDQMDDDKDSEDDILIKGEEIKK +SKKKRQRLDNSTKEFLEKVFEKNKQPNRRERELIAEKHGVSLSQIRVWFTNKRMRKKEPKSKAKSSSNST + +>CAE84400.1 MATa1 protein [Nakaseomyces delphensis] +MNVQEIHNIREACITILSGTKHNSVLFEPCDKFDEVINSLDIDPDSKETLRRSVSLLVGTRNHKRGCNID +KKTKDMLNKVYEQKQYLTKEEREFVAKKCNLTPLQVRVWFANKRIRNKNTKM + +>CAE84408.1 MATa1 transciption factor [Nakaseomyces delphensis] +MNVQEIHNIREACITILSGTKHNSVLFEPCDKFDEVINSLDIDPDSKETLRRSVSLLVGTRNHKRGCNID +KKTKDMLNKVYEQKQYLTKEEREFVAKKCNLTPLQVRVWFANKRIRNKNTKM + +>CAE84411.1 A1 transcription factor [Kluyveromyces lactis] +MCDNDMADIQSKLSSFCEEIRALALKEGYNLEGDKSPSSKPYFMSWPKEIDVNHPNFAFFTKLQFQYDKS +LETILNSCYLQELQLDPTRIIETLQQHFNNSILRYADIENISDVKDESPMSFDTEHECTDTSEDISDKSE +ISSTNSDNPIQNCYPTYKRSFIKHESRGILEKIFKVKQCPNTSERLYIAQKLDLTPSQVRIWFTNKRMRA +KKHTTGKGTKRKSKKYPS + +>CAE84419.1 MATa1 transcription factor [Ogataea angusta] +MQFTILNEPSLDSQRREGDLASENYVFGDIRKEGVRILEDSLRSERNALLAIGAGGAADRVKQHYRKLLD +DWEAAIMEIRSNDKYESLCCGFAIEKAEVETEMRNTEELDVYKECLVSECFDKKKRRHIPESSKELLEKA +FKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA + +>CAE84422.1 MATA1 transcription factor [Yarrowia lipolytica] +MPSRTPTDIWRCQRLILAARKGETTCQALHEQSIEISSSLKWFEEISEWMSKFPESGLYISTTIWTFLEG +VPNEHRIKYDRLIRHYITYIQQLVPARLVTAYLDSLFKICSHPTRTERRIIGEHCGISLHQVTQWFTNRR +FREPKTDSPDDGSTRFIPSPMTSPGSIDECSFPESP + +>CAA07612.1 MATA1 [Yarrowia lipolytica] +MPSRTPTDIWRCQRLILAARKGETTCQALHEQSIEISSSLKWFEEISEWMSKFPESGLYISTTIWTFLEG +VPNEHRIKYDRLIRHYITYIQQLVPARLVSILKTRAVLGNTKTERMDDQ + +>BAM78610.1 a1 transcriptional factor [Zygosaccharomyces rouxii] +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIF +EGNSSKNSSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWFNNRR +MRSK + +>BAM78617.1 a1 transcriptional protein [Zygosaccharomyces rouxii] +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIF +EGNSSKNSSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWFNNRR +MRSK + +>BAM78621.1 a1 transcriptional factor [Zygosaccharomyces rouxii] +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIF +EGNSSKNLSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWFNNRR +MRSK + +>XP_003870289.1 Mtla1p [Lodderomyces orthopsilosis Co 90-125] +MNSEYHKGKALLDSLTVLEELTKTTTLIGPTKEQGNSDTTDNPHQLLQDLKENWNSIDPRVEWGLKQLPS +NLRTIERFEQEDMVSIPLQSNAKFLLRRVSISVRNSTTKKLTLHDTYLCGSWNALKIPRQRLSLASKELL +ERIFLVKQSPNREERGLIAEKCGVSPLQIRVWFTNKRMRSKRQ + +>BAO37764.1 hypothetical protein KLMA_10142 [Kluyveromyces marxianus DMKU3-1042] +MDGHDKKNPILLLKELCQEAQGLAGVQLKEDGFSSPFKTFSIPFPEEAVKKCSNKALLLQLYHQYSTSLE +TILNKCPLEDLKVDPQMVADAFNNQFLRTIQLLPVENNLKIKEQNPSDSSGKEDSKNDTVEEDSPSPDNQ +NSRTAKKFFLEPEAKDLLEKVFKVKKCPNTSERLFIAQKLGLTASQVRIWFTNKRMRTKSKQKKVKPIAG +PIK + +>BAO39317.1 mating-type protein A1 [Kluyveromyces marxianus DMKU3-1042] +MDGHDKKNPILLLKELCQEAQGLAGVQLKEDGFSSPFKTFSIPFPEEAVKKCSNKALLLQLYHQYSTSLE +TILNKCPLEDLKVDPQMVADAFNNQFLRTIQLLPVENNLKIKEQNPSDSSGKEDSKNDTVEEDSPSPDNQ +NSRTAKKFFLEPEAKDLLEKVFKVKKCPNTSERLFIAQKLGLTASQVRIWFTNKRMRTKSKQKKVKPIAG +PIK + +>AAD51404.1 MTLa1 [Candida albicans] +MNSEIESSLTLLKSVEKLVQATSVYKNEDNEEIFLQLKRERQENSNSHEETETFFHESLDRLMKLSSIAG +SKVQYELSLNNLYNLLLRTRKQEEEEAFIFPTVSTEEFALEQITFEVSDQMDDDKDSEDDILIKGEEIKK +SKKKRQRLDNSTKEFLEKVFEKNKQPNRRERELIAEKHGVSLSQIRVWFTNKRMRKKEPKSKAKSSSNST + +>CDM87353.1 hypothetical protein [Zygosaccharomyces sapae] +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIF +EGNSSKNSSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWVCNSG + +>XP_007375272.1 Mtla1p, partial [Spathaspora passalidarum NRRL Y-27907] +QNEFYIRNLYKNILTPQHENYSFQARPLTPDDFHLSKITISVSNTLDDEDQDQDDVQATSSNSSRTKQTR +RRLDSSVKEFLESVFINHPSPNRKERELIAKKCGVSPLQIRVWFTNKRMRRKQTNYNNNNSNDNDEII + +>sp|Q707Y3.1|MATA1_YARLL RecName: Full=Mating-type protein A1; AltName: Full=MATA1 transcription factor +MPSRTPTDIWRCQRLILAARKGETTCQALHEQSIEISSSLKWFEEISEWMSKFPESGLYISTTIWTFLEG +VPNEHRIKYDRLIRHYITYIQQLVPARLVTAYLDSLFKICSHPTRTERRIIGEHCGISLHQVTQWFTNRR +FREPKTDSPDDGSTRFIPSPMTSPGSIDECSFPESP + +>sp|Q707Y6.1|MATA1_PICAN RecName: Full=Mating-type protein A1; AltName: Full=MATa1 transcription factor +MQFTILNEPSLDSQRREGDLASENYVFGDIRKEGVRILEDSLRSERNALLAIGAGGAADRVKQHYRKLLD +DWEAAIMEIRSNDKYESLCCGFAIEKAEVETEMRNTEELDVYKECLVSECFDKKKRRHIPESSKELLEKA +FKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA + +>sp|Q708A1.1|MATA1_NAKDE RecName: Full=Mating-type protein A1; AltName: Full=MATa1 transcription factor +MNVQEIHNIREACITILSGTKHNSVLFEPCDKFDEVINSLDIDPDSKETLRRSVSLLVGTRNHKRGCNID +KKTKDMLNKVYEQKQYLTKEEREFVAKKCNLTPLQVRVWFANKRIRNKNTKM + +>sp|Q9HG12.1|MATA1_KLULA RecName: Full=Mating-type protein A1; AltName: Full=A1 transcription factor; AltName: Full=MATa1 protein +MCDNDMADIQSKLSSFCEEIRALALKEGYNLEGDKSPSSKPYFMSWPKEIDVNHPNFAFFTKLQFQYDKS +LETILNSCYLQELQLDPTRIIETLQQHFNNSILRYADIENISDVKDESPMSFDTEHECTDTSEDISDKSE +ISSTNSDNPIQNCYPTYKRSFIKHESRGILEKIFKVKQCPNTSERLYIAQKLDLTPSQVRIWFTNKRMRA +KKHTTGKGTKRKSKKYPS + +>AMK38000.1 a1 transcriptional factor [Zygosaccharomyces rouxii] +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIF +EGNSSKNSSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWVCNSG + +>AMK38002.1 a1 transcriptional factor [Zygosaccharomyces rouxii] +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIF +EGNSSKNSSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWVCNSG + diff --git a/results/2026-10-30_mat_hmms/data/public_a2.faa b/results/2026-10-30_mat_hmms/data/public_a2.faa new file mode 100644 index 00000000..217973a4 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/public_a2.faa @@ -0,0 +1,265 @@ +>SCV12319.1 Mating-type protein a2 [Komagataella phaffii CBS 7435] +MTVTTFLSSSFDSVAFAMTCVSDKSYKTTAGKGLSGKKFNLSTHRPRNKFIISKSIFSKVIQRYYPDLSF +EENSKIASYLWKHRFGCFDKYYEYLQIEESKFTSLVTSKIDQLAFKAVPTEKTTSQCTFRFSSSPKRRRT +VNLNILKPLCKRVYSQQFHSPLTGITTQDIFVAEENNV + +>AOW29606.1 Mtla2p [Candida albicans SC5314] +MPYTFNFPKSQSSFKYSIKSLTNSRSFPMISIYVTPQSTPKFINNQSIDKMTVLSSFRSRNSFIIARSIL +SKLLRKNKADFKKVSKSVSLLWGSVDNSFKNYFEYLSILESQWHDKKSSNLNPISSTRTTSELNYIELCS +SIYNQSRKHPRSVQGNKAKFRLYKNVSKKSNRKPRRLKNGFKVSRSTINCSYGIYTEDVFQ + +>XP_019330960.1 Mtla2p [Candida albicans SC5314] +MPYTFNFPKSQSSFKYSIKSLTNSRSFPMISIYVTPQSTPKFINNQSIDKMTVLSSFRSRNSFIIARSIL +SKLLRKNKADFKKVSKSVSLLWGSVDNSFKNYFEYLSILESQWHDKKSSNLNPISSTRTTSELNYIELCS +SIYNQSRKHPRSVQGNKAKFRLYKNVSKKSNRKPRRLKNGFKVSRSTINCSYGIYTEDVFQ + +>XP_022673680.1 mating-type protein A2 [Kluyveromyces marxianus DMKU3-1042] +MSNSTLRRTTFFKLSTKEEACSSNKAVQSNNNSIEFPNLKRNGKAFTCSSNEKTSKKFTRPRNQFVLMRT +LFNRCVTTHILEHFNQTKIQKNMFSVTSKITSELWNESPSELKVYFLLLATLEENWHKNKHYCSWDKQSS +QTLSMEPIENSHIFSRLAISLSMGVGSAVSSYSAKDLCLFPKTRTKKKRVASPTLLPNQRFNSKFNSKLS +QDTKTKSPKSLVRLKSTKLPTQSGKVFKKRYQNENRVIEDLFLV + +>XP_022675169.1 mating-type protein A2 [Kluyveromyces marxianus DMKU3-1042] +MSNSTLRRTTFFKLSTKEEACSSNKAVQSNNNSIEFPNLKRNGKAFTCSSNEKTSKKFTRPRNQFVLMRT +LFNRCVTTHILEHFNQTKIQKNMFSVTSKITSELWNESPSELKVYFLLLATLEENWHKNKHYCSWDKQSS +QTLSMEPIENSHIFSRLAISLSMGVGSAVSSYSAKDLCLFPKTRTKKKRVASPTLLPNQRFNSKFNSKLS +QDTKTKSPKSLVRLKSTKLPTQSGKVFKKRYQNENRVIEDLFLV + +>BBB04550.1 mating type a2 [Zygosaccharomyces sp. NBRC 1876] +MDFASECVLPKVVVKTESHKDFLFFKNKKKPQSNSLQFKFVGQKVNETNRVAEINLSSEVNKGSSKETNQ +IITRPRNCFIIMRSIFHNVIVRSLQKYEISSLQHVSAITSQLWGKNDGIFQLYFELLSQFEEHWHLNIYP +EYRYHKVNKISRQLENKLVYQNMLDRMRYFTASSLIANLEDILILPPAHPPPSSSTYTYPALRRPAAAAA +AKAQAQAQAQAQAANAQAANPAAPAPPATNAERNAKTKPAPGGKVSKQRIHPKKKPSPPPRLHRPASAVQ +QIAFFETFHQQNFNTPRFSFTVYSC + +>AXL14413.1 MATa2 transcription factor, partial [Zygosaccharomyces pseudobailii] +MEFASE + +>ABN68300.2 mating locus regulatory protein [Scheffersomyces stipitis CBS 6054] +MSSSFSFSFPKVNRGTSTGSSVKQLACASSFPLVSTNSSKRDSDFLTHENPSSLLKKSRSRNDTFRSRNS +FIVARNIITNLMPSDLKTFRDLAKTVSSIWKKSPRQFQEYFQYLSLVEASWYEKSTGTIRSAPPAFKHSE +YRQLCRSSYYTKSKRDSAPKQSADSFKDKFRVTKVKRERNKGRSQTGAFKIKTSSVNCSLGIFTEDMYK + +>CAO02579.1 mating type a2 protein [Lachancea waltii] +MESEAWTQGYVECQVPRKCQNSREIKKGIQKHNTFPSIGREQAHLLNYQFISDHKKTKQNRSPSSKPKNK +FILMRSSLHGVIQRLQFFSEAESLKESKVELVSKIASKLWRINKGAFQTYHELLAQFDEYKSFNKPTNKL +SLKKLTEAIGRECIPSTYSYETLTNGAHTTAIESKKTYKAFCGRFSLNEATGRVRKKKPIQTFALRSANI +EDVFLI + +>XP_001386329.2 Mtla2p [Scheffersomyces stipitis CBS 6054] +MSSSFSFSFPKVNRGTSTGSSVKQLACASSFPLVSTNSSKRDSDFLTHENPSSLLKKSRSRNDTFRSRNS +FIVARNIITNLMPSDLKTFRDLAKTVSSIWKKSPRQFQEYFQYLSLVEASWYEKSTGTIRSAPPAFKHSE +YRQLCRSSYYTKSKRDSAPKQSADSFKDKFRVTKVKRERNKGRSQTGAFKIKTSSVNCSLGIFTEDMYK + +>QGN14065.1 mating-type protein A2 [Kluyveromyces marxianus] +MSNSTLRRTTFFKLSTKEEACSSNKAVQSNNNSIEFPNLKRNGKAFTCSSNEKTSKKFTRPRNQFVLMRT +LFNRCVTTHILEHFNQTKIQKNMFSVTSKITSELWNESPSELKVYFLLLATLEENWHKNKHYCSWDKQSS +QTLSMEPIENSHIFSRLAISLSMGVGSAVSSYSAKDLCLFPKTRTKKKRVASPTLLPNQRFNSKFNSKLS +QDTKTKSPKKLG + +>QGN15037.1 mating-type protein A2 [Kluyveromyces marxianus] +MSNSTLRRTTFFKLSTKEEACSSNKAVQSNNNSIEFPNLKRNGKAFTCSSNEKTSKKFTRPRNQFVLMRT +LFNRCVTTHILEHFNQTKIQKNMFSVTSKITSELWNESPSELKVYFLLLATLEENWHKNKHYCSWDKQSS +QTLSMEPIENSHIFSRLAISLSMGVGSAVSSYSAKDLCLFPKTRTKKKRVASPTLLPNQRFNSKFNSKLS +QDTKTKSPKSLVRLKSTKLPTQSGKVFKKRYQNENRVIEDLFLV + +>XP_034013595.1 Mtla2p [Diutina rugosa] +MDEDTPLSAHVFQLQKKSVQVSHTLKDLLSPDSFPMKAINAVSEWQVETQFINYFKPEDLNKKTAVEKTE +STFRTPNRYLLMRSTIGGLYRLRQKPPTRLLSKITSSLWKKSSKNTHAYYEYLAQNHAAWHEHYMRYEKI +STRKWRPVDFKKQCLTFKITIRNSRKLNQLKSVES + +>XP_036664546.1 Mtla2p [Lodderomyces parapsilosis] +MRGYSFSFPKIEKNILKGVKDFTTIDSFPISSINVTPKKVVSKFVQNSPSLAENENKFRSRNSFIIARST +LSSLLKENTYELKIVSKGVSNLWSHVDTSFKTYFKYLSLIESMWHDKKRSSYNSILRVNKVHTLTNSEHS +SSPLSRDNNAHDSMPQRHRFRVKKLSGRTKRPKIRNYESGFKISSTSLSCNYGIVTEDIFK + +>BDH85264.1 transcription factor, partial [Zygosaccharomyces sp.] +MEFVSECILPKATVNTESHKDFLFFKNKKKSQSSSLQFKFVGQKVNETNRVAEINLSSEKSKSNTKETNQ +VITRPRNCFIIMRSIFHNVIVRSLQEYEISSLQHVSAITSQLWGKNDGIFQLYFELLS + +>BDH85266.1 transcription factor, partial [Zygosaccharomyces sp.] +MEFVSECILPKATVNTESHKDFLFFKNKKKSQSSSLQFKFVGQKVNETNRVAEINLSSEKSKSNTKETNQ +VITRPRNCFIIMRSIFHNVIVRSLQEYEISSLQHVSAITSQLWGKNDGIFQLYFELLS + +>BDH85268.1 transcription factor, partial [Zygosaccharomyces sp.] +MEFVSECILPKATVNTESHKDFLFFKNKKKSQSSSLQFKFVGQKVNETNRVAEINLSSEKSKSNTKETNQ +VITRPRNCFIIMRSIFHNVIVRSLQEYEISSLQHVSAITSQLWGKNDGIFQLYFELLS + +>BDH85269.1 transcription factor [Zygosaccharomyces sp.] +MEFVSECILPKATVNTESHKDFLFFKNKKKSQSSSLQFKFVGQKVNETNRVAEINLSSEKSKSNTKETNQ +VITRPRNCFIIMRSIFHNVIVRSLQEYEISSLQHVSAITSQLWGKNDGIFQLYFELLSQFEEHWHLNIYP +EYRYHKVNKISRQLENKLVYQNMLDRMRYFTASSLIANLEDILILPPAHPPPSSSTYTYPALRRPAAAAA +AAKAQAQAQAQAQAANAQAANPAAPAPPATNAERNAKTKPAPGGKVSKQRIHPKKKPSPPPRLRRPASAV +QQIACQTFKSSTWLNVEDLFIPNQ + +>CAH2450367.1 Mating-type protein a2 [Komagataella phaffii CBS 7435] +MTVTTFLSSSFDSVAFAMTCVSDKSYKTTAGKGLSGKKFNLSTHRPRNKFIISKSIFSKVIQRYYPDLSF +EENSKIASYLWKHRFGCFDKYYEYLQIEESKFTSLVTSKIDQLAFKAVPTEKTTSQCTFRFSSSPKRRRT +VNLNILKPLCKRVYSQQFHSPLTGITTQDIFVAEENNV + +>XP_051615100.1 Mtla2p [Lodderomyces pseudojiufengensis] +MIVHTFNFPKCRPEEVTNIKCLTNRESFPAISVNVTPNSYKSDFIQVASPSGNSDEEKITFRSRNNFIIA +RSTLANLVQEKQSNFEKVSKLVSIVLNVSFKLYFEYLAILESYWYEKKSSTLHSPKKAKNIVGIKYSEIF +TSLYPESSVETKMKPQKHRFRLTNAPVRTRKRLVKKKESVKGFKIGKTSYSCNYGILTQDIFKEELAKGE +SLLNDINELKQLTSLIREFGEQENQSTQIGNDLNEKYFDPRLDWALSQLQSTLETIRNFEGESSLLVSSE +SAQEFELNQISITVSGTDKLMETVNNSADRKVTTELGKPRKRLASSAKNLLEQIFLVQQTPNRRERELIA +RKCGVTPLQIRVWVCIYDQNFYKV + +>XP_062812647.1 Mtla2p [Priceomyces carsonii] +MDSSDFRRACYKTSFILPRSSCKKIENSQQLSNFVTNTSFPSISTNSKYCPLFDESKFINCNVLSKEANR +DRPREDTFRSRNRFIIARNVLGRMIKEKQKDVNSISKIVSIVWKNCGRNLHDYFEYLSLLEGYWNDCLRG +GTKRFGLAPKKDASNKLSYSKVCTSSYLKTSSKRKPIQENSTKTSINSRFRIAIDLKVVKKKAKRKSKTP +PLTDNFRIESCSVTTWFGILTEDMFLNDF + +>CAK7898773.1 MATa2 protein [[Candida] anglica] +MEFVNQDIYCRHLDSTTPHSVYKTFFPPLIEKSTTHTISSISNSTSFPLIATSTRIKNLQPDDNFRVYTS +FPCRKPNKVKKDSAEQNYTGGRNKFIIARNLLSQLVRDQGLISRRDVSKTTSLVWMSGSSQFQSYFAYLS +ILDTSWNEFKSILQRCRTAEAVENIEWKGVLSHNSAFCSYTKDKVALFKRETPNVSFKVVRRKFDITKPK +LRKKSKRINQDFLSNSCINFIEDIYK + +>XP_064741611.1 Mtla2p [Scheffersomyces amazonensis] +MTVEGLYTFQFQRTNRPRVIETVRDIMDQESFPLYETSGFNEGIPEVYEFSEVDESSNDESDIYRTRNPF +LILRSIVSGFLSLSSGDYKFISKVSSIMWQDGNTLYKDYYEYISTIDSFCFSQKFRAVLQEAPVKTLSIK +TAYPGLCSYNYLKDPELEPETSPSKKFRVKYKSLQQSRTKLRNLRRLLRLRQYPLNRRPGTLTEDVFISQ +CM + +>XP_064762304.1 Mtla2p [Scheffersomyces coipomensis] +MKSHSFSFPEKASKSNKKGSSNKLKVFTHSKSYPLISTSVDGSFSFADSSFLSPEVEDPKSKENNFRSRN +KYFVARNALGKLISSEYGDYRFVSKVTALMWQQCSKRFNGYCEYLSAIDSFCYDELSSKKDDEEEARYKM +SSKEYQLTCRCVYMRKEVDSNSRTSHAIRFRVQKNQVKRPRHRIIKLSSKSQLQHYVFNRKLGIFTEDVF +T + +>XP_067547707.1 Mtla2p [Lodderomyces metapsilosis] +MLPQTKASPDSFNSLQKLQRRQANFDREIALLLLEVPFPLWGQADTSFKIYFEYLSITESAWYDRKKMAY +NSKLQNNKLYGQDSKGSLMTVEILKKLNQSVPKKHRFRLKKSANRTKRPKIRRYTTGFKISSTSLSCNYG +IVTEDVFK + +>XP_460137.2 Mtla2p [Debaryomyces hansenii CBS767] +MSLSPDKIFTFCFKLPRRIDIPVESNQPTTKKNFKIKELTTTNSYPSISINIKQYVSFTSEKYIEASRKP +TAKDKKTAITRGSFRTRNKFIIARNLLCRLVKHEKALSSTEISKVVSLVWKESNKSLHDYFEYLSLLETY +WYDKLMSSYGNSTLQATATAAVSIRLSSVPYENLCASCYNKHKQKKTLNYDYSKSAKFRVHKDFKEKKKT +FLTIKDHSKKLEKLSDAFRINQCTITTNFGIVTEDMFFNSL + +>BGD02940.1 transcriptional factor 2 [Zygosaccharomyces sp.] +MEFVSECILPKATVNTESHKDFLFFKNKKKSQSSSLQFKFVGQKVNETNRVAEINLSSEKSKSNTKETNQ +VITRPRNCFIIMRSIFHNVIVRSLQEYEISSLQHVSAITSQLWGKNDGIFQLYFELLSQFEEHWHLNIYP +EYRYHKVNKISRQLENKLVYQNMLDRMRYFTASSLIANLEDILILPPAHPPPSSSTYTYPALRRPAAAAA +AAKAQAQAQAQAQAANAQAANPAAPAPPATNAERNAKTKPAPGGKVSKQRIHPKKKPSPPPRLRRPASAV +QQIACQTFKSSTWLNVEDLFIPNQ + +>CAO2664331.1 Mating-type-like protein A2 [Diutina rugosa] +MDEDTPLSAHVFQLQKKSVQVSHTLKDLLSPDSFPMKAINAVSEWQVETQFINYFKPEDLNKKTAVEKTE +STFRTPNRYLLMRSTIGGLYRLRQKPPTRLLSKITSSLWKKSSKNTHAYYEYLAQNHAAWHEHYMRYEKI +STRKWRPVDFKKQCLTFKITIRNSRKLNQLKSVES + +>KAM3161349.1 Mating type a2 protein [Lachancea thermotolerans] +MILSELNKVENMNWTEGYAECHLPKVANQIKYKFGAQKTLGKDRFPCIAQRKCPKQICHFVTSGKTDKIK +QSGLKSRNKFILMRGLLHCMVQQLRNCSDPFNANRAKVEEVSKIASAIWRTNKGIFQSYHEMLAVFEDSK +STNITISKPTLKKLTEAIGRECIPSTYSYETLTNGADTTAMESKKTYKAFCGRFSLKEATGRVRKKKPTQ +TFALRSAKIEDIFLI + +>YCL22009.1 Mating-type protein a2 [Komagataella phaffii] +MTVTTFLSSSFDSVAFAMTCVSDKSYKTTAGKGLSGKKFNLSTHRPRNKFIISKSIFSKVIQRYYPDLSF +EENSKIASYLWKHRFGCFDKYYEYLQIEESKFTSLVTSKIDQLAFKAVPTEKTTSQCTFRFSSSPKRRRT +VNLNILKPLCKRVYSQQFHSPLTGITTQDIFVAEENNV + +>ADY62667.1 MTLa2 [Lodderomyces metapsilosis] +MTHGQHSSTVQVLYDFIVNMCSSSTINYEIMNSDIQNITKSIELEISNSREDKTDANTLIAINKCTRLLE +CITLKRLELSGRPSKLQIRIQQPSKLLSHMHKTACNGRVQKISDEKLKNDEVTKAHKRLLKNNLEILENW +FDENFTDPYLDSASLEFLIRETGLSKSQIQNWVSNRRRKERNVKITPEIAELIA + +>ADY62676.1 MTLa2 [Lodderomyces orthopsilosis] +MFSYKFCFPKVGKEFPKRIKDFTDNNSFPILSINVTLNKTIPDFVYFSTASAEKGNKFRSRNSFIIARST +LSSMLKKNIYELQTVSKGVSQLWSHVDSSFKSYFEYLSVIESIWYDRKIFHNLTQPNNKIYTPKNSGLFA +SSQNFKKKDENMPGKHRFRLKRSIGRIKRPKVKRFTNGFKISSTSMSCNYGILTEDVFK + +>ADY62680.1 MTLa2 [Lodderomyces orthopsilosis] +MTTVGQASTVQLLHNYIVNLCSPNTVNYEIINKDIRKIIESLEIDVLVNSKDKTDTKTLNVVNICARLLK +YIISERLKLSRRSARLQKEEQPIPNHQFYIQKRSEYNGRIQKNTTKNFKNETLSKPSKRLSKNNLKVLEN +WFDENFNDPYLNNISLRLLITTTNLSKSQIQNWVSNRRRKERRVKISPEIMELLT + +>ADY62690.1 MTLa2 [Lodderomyces orthopsilosis] +MFSYKFCFPKVSKEFPKRIKDFTDDNSFPILSINVALNKTTPDFVHFSTASTEKGNKFRSRNSFIIARST +LSSMLKKNIYELQTVSKGVSQLWSHVDSSFKTYFEYLSVTESIWYDRKIFHNSTQQNNKNYIPKNSGLFA +SRQNFKKKDENMPKKHRFRLKKSIGRIKRPKVRRFTNGFKISSTSMSCNYGILTEDVFK + +>ADY62696.1 MTLa2 [Lodderomyces orthopsilosis] +MTTVDQASTVQLLHNYIVNLCSPNTVNYETINKDIRKIIKSLEIDVLVNSNGKTDTKTLNIVNKCARLLK +YIISERLKLSRRSPRLQNEKQPISNHQFYIQKRSEYNGRVQKNTTKNFKNETLSKPSKRLSKNNLEVLEN +WFDENINDPYLNNISLRLLITTTNLSKSQIQNWASNRRRKERSVKISPEITEFFT + +>CCA89253.1 mating-type a2 protein [Kluyveromyces marxianus] +MSNSTLRRTTFFKLSTKEEACSSNKAVQSNNNSVEFPNLRRNGKAFTCSSNEKTSKKFTRPRNQFVLMRT +LFNRCVTTHILEHFNQTKVQKNMFSVTPKITSELWNESPSELKVYFLLLATLEEKWHKNKHYCSWDKQSS +QTLSMEPIENSHIFSRLAISLSMGVGSAVSSYSAKDLCLFPKTRTKKKRVASPTLLPNQRFNSKFNSKLS +QGTKTKSPTSLVRLKSTKFPTQSGKVFKKRYQNENRVIEDLFLV + +>sp|P0CY12.1|MATA2_YEASX RecName: Full=Putative mating-type protein A2; Short=MATa2 protein +MRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKG +LENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>CAE84410.1 A2 HMG-domain transcription factor [Kluyveromyces lactis] +MANSTLRRTTFFKLTTTEDEDTIPKLLQPNNNSVAFPNLKRTGKAFTNDTIKENTKKYTRPRNQFVLMRT +LFNRRVNNHILQYYNKSKLEKKMFTLTSKITSELWNESSPDLKSYFSLLATLEENWHKYTHYCSWDRNSA +QTLSMEPIELSQVRPRLISSLTVGAGSSVSTYTLRELLLVRKIKSRKRKTTTLTQDSSPTTKFKYKFKKQ +PKTKMNSNLSKLRFKSKQPPTPPEENSNVFKKRYTSENRIIEDLFLM + +>CAE84423.1 MATA2 transcription factor [Yarrowia lipolytica] +MENTILHIHSFQLPQTEQPYPEAMLFDRDTSDSRTVLTQKPNGLEISLNFLQYDGHKGLFIRQDSRTNEP +EYIEPKVLKPRNSYILFRNATSRCSQSIDPNSAFVSRVSSYIWGSGIPNPIVRRWFKYMGFFEALYHEVD +YPEYIPNKLPKSPKKPKVQKGATKSKKKGAKGKNKVTALQVLVGPTVGVGGRMTVSPMTAFFSNNSHVTC +YDPNFVLDTPTREFLMMPLDDITLPSQGPRSDSQEQHVPRQPPDGQDYFDILDIDDFVSPYDDLTTRSFT +NRQLFKHATLG + +>CAA07613.1 MATA2 [Yarrowia lipolytica] +MENTILHIHSFQLPQTEQPYPEAMLFDRDTSDSRTVLTQKPNGLEISLNFLQYDGHKGLFIRQDSRTNEP +EYIEPKVLKPRNSYILFRNATSRCSQSIDPNSAFVSRVSSYIWGSGIPNPIVRRWFKYMGFFEALYHEVD +YPEYIPNKLPKSPKKPKVQKGATKSKKKGAKGKNKVTALQVLVGPTVGVGGRMTVSPMTAFFSNNSHVTC +YDPNFVLDTPTREFLMMPLDDITLPSQGPRSDSQEQHVPRQPPDGQDYFDILDIDDFVSPYDDLTTRSFT +NRQLFKHATLG + +>XP_003870288.1 Mtla2p [Lodderomyces orthopsilosis Co 90-125] +MFSYKFCFPKVSKEFPKRIKDFTDDNSFPILSINVALNKTTPDFVHFSTASTEKGNKFRSRNSFIIARST +LSSMLKKNIYELQTVSKGVSQLWSHVDSSFKTYFEYLSVTESIWYDRKIFHNSTQQNNKNYIPKNSGLFA +SRQNFKKKDENMPKKHRFRLKKSIGRIKRPKVRRFTNGFKISSTSMSCNYGILTEDVFK + +>AAT08437.1 MTLa2 [Candida albicans] +MPYTFNFPKSQSSFKYSIKSLTNSRSFPMISIYVTPQSTPKFINNQSIDKMTVLSSFRSRNSFIIARSIL +SKLLRKNKADFKKVSKSVSLLWGSVDNSFKNYFEYLSILESQWHDKKSSNLNPISSTRTTSELNYIELCS +SIYNQSRKHPRSVQGNKAKFRLYKNVSKKSNRKPRRLKNGFKVSRSTINCSYGIYTEDVFQ + +>BAO37765.1 mating-type protein A2 [Kluyveromyces marxianus DMKU3-1042] +MSNSTLRRTTFFKLSTKEEACSSNKAVQSNNNSIEFPNLKRNGKAFTCSSNEKTSKKFTRPRNQFVLMRT +LFNRCVTTHILEHFNQTKIQKNMFSVTSKITSELWNESPSELKVYFLLLATLEENWHKNKHYCSWDKQSS +QTLSMEPIENSHIFSRLAISLSMGVGSAVSSYSAKDLCLFPKTRTKKKRVASPTLLPNQRFNSKFNSKLS +QDTKTKSPKSLVRLKSTKLPTQSGKVFKKRYQNENRVIEDLFLV + +>BAO39316.1 mating-type protein A2 [Kluyveromyces marxianus DMKU3-1042] +MSNSTLRRTTFFKLSTKEEACSSNKAVQSNNNSIEFPNLKRNGKAFTCSSNEKTSKKFTRPRNQFVLMRT +LFNRCVTTHILEHFNQTKIQKNMFSVTSKITSELWNESPSELKVYFLLLATLEENWHKNKHYCSWDKQSS +QTLSMEPIENSHIFSRLAISLSMGVGSAVSSYSAKDLCLFPKTRTKKKRVASPTLLPNQRFNSKFNSKLS +QDTKTKSPKSLVRLKSTKLPTQSGKVFKKRYQNENRVIEDLFLV + +>sp|O93958.1|MATA2_YARLL RecName: Full=Mating-type protein A2; AltName: Full=MATA2 transcription factor +MENTILHIHSFQLPQTEQPYPEAMLFDRDTSDSRTVLTQKPNGLEISLNFLQYDGHKGLFIRQDSRTNEP +EYIEPKVLKPRNSYILFRNATSRCSQSIDPNSAFVSRVSSYIWGSGIPNPIVRRWFKYMGFFEALYHEVD +YPEYIPNKLPKSPKKPKVQKGATKSKKKGAKGKNKVTALQVLVGPTVGVGGRMTVSPMTAFFSNNSHVTC +YDPNFVLDTPTREFLMMPLDDITLPSQGPRSDSQEQHVPRQPPDGQDYFDILDIDDFVSPYDDLTTRSFT +NRQLFKHATLG + +>sp|Q71U11.1|MATA2_CANAL RecName: Full=Mating-type-like protein A2; Short=MTLa2 protein +MPYTFNFPKSQSSFKYSIKSLTNSRSFPMISIYVTPQSTPKFINNQSIDKMTVLSSFRSRNSFIIARSIL +SKLLRKNKADFKKVSKSVSLLWGSVDNSFKNYFEYLSILESQWHDKKSSNLNPISSTRTTSELNYIELCS +SIYNQSRKHPRSVQGNKAKFRLYKNVSKKSNRKPRRLKNGFKVSRSTINCSYGIYTEDVFQ + +>sp|Q9HG13.1|MATA2_KLULA RecName: Full=Mating-type protein A2; AltName: Full=A2 HMG domain transcription factor +MANSTLRRTTFFKLTTTEDEDTIPKLLQPNNNSVAFPNLKRTGKAFTNDTIKENTKKYTRPRNQFVLMRT +LFNRRVNNHILQYYNKSKLEKKMFTLTSKITSELWNESSPDLKSYFSLLATLEENWHKYTHYCSWDRNSA +QTLSMEPIELSQVRPRLISSLTVGAGSSVSTYTLRELLLVRKIKSRKRKTTTLTQDSSPTTKFKYKFKKQ +PKTKMNSNLSKLRFKSKQPPTPPEENSNVFKKRYTSENRIIEDLFLM + +>AAY33181.1 MTLa2 [Lodderomyces parapsilosis] +MRGYSFSFPKIEKNILKGVKDFTTIDSFPISSINVTPKKVVSKFVQNSPSLAENENKFRSRNSFIIARST +LSSLLKENTYELKIVSKGVSNLWSHVDTSFKTYFKYLSLIESMWHDKKRSSYNSILRVNKVHTLTNSEHS +SSPLSRDNNAHDSMPQRHRFRVKKLSGRTKRPKIRNYESGFKISSTSLSCNYGIVTEDIFK + diff --git a/results/2026-10-30_mat_hmms/data/public_alpha2.faa b/results/2026-10-30_mat_hmms/data/public_alpha2.faa new file mode 100644 index 00000000..5caf7d89 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/public_alpha2.faa @@ -0,0 +1,996 @@ +>pdb|1APL|C Chain C, PROTEIN (MAT-ALPHA2 HOMEODOMAIN) +TKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELA +DLLSGEPLAKKKE + +>pdb|1APL|D Chain D, PROTEIN (MAT-ALPHA2 HOMEODOMAIN) +TKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELA +DLLSGEPLAKKKE + +>AAG21091.1 alpha2p [Kluyveromyces lactis] +MSRIPIHSLLNPSESCKSISNVPSNYRDLSTFNKERAKVITTFQEMFYSMLENNDDYNKIESLIRNFQPK +LTWSHKCESLTFKQKAYLTAIIQKSIKSLLVLLKEKGKMREIEFSRKEVRKINKYRQSSKNFESVNIKIL +TQDLMHSNNNEFKKGKRFLKSHIQLLENWYSMNRRNPYLAENDLAYISKNTTLTKTQIKNWLANRRRKDK +ITEVSSDIRNILN + +>XP_018223412.1 homeodomain mating type protein alpha2 DI49_0790 [Saccharomyces eubayanus] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>SCV12330.1 Mating-type protein alpha2 [Komagataella phaffii CBS 7435] +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRA +IALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLS +KTQIRNWISNKRRKERSSTVSTELLQALNKSPS + +>BAX02933.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKED +QKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRS +NKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVS +ELVNGSKQGPEGSGT + +>BAX02941.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFRRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAX02943.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKED +QKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRS +NKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVS +ELVNGSKQGPEGSGT + +>SMN20089.1 MATALPHA2 [Maudiozyma saulgeensis] +MNKIPIRDLLNPASKRRQYKPELKQINTKLIHICSSLPEGIFENIKEIKMDLHEIITKLSIIKREGRLDS +EERYLIKISFQLATIVANFLNSNTQENIKPIKEQKKKKIEKKIPKEDEVVFNVVTQNMMNMDNVKQNSFR +GHRFSKQNVEVLEEWYSIHKHKPYLDKKSTEVLQYQTQLSRTQIKNWVSNKRRKEKTVQVSSEILQLING +K + +>GAX72286.1 homeodomain mating type protein alpha2 [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>BBB04546.1 mating type alpha2 [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BBB04548.1 mating type alpha2 [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BBB04552.1 mating type alpha2 [Zygosaccharomyces sp. NBRC 1876] +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKED +QKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRS +NKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVS +ELVNGSKQGPEGSGT + +>pdb|1YRN|B Chain B, PROTEIN (MAT ALPHA2 HOMEODOMAIN) +TKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELA +DLLSGEPLAKKKE + +>BBD18018.1 putative transcription factor [Ogataea minuta] +MKTNKCLMQRYNENEDNPYLTRTDLDEIVKQTGLSSSQVKNWVSNKRRKEKTVFVSEELCQLLY + +>AXK50429.1 MATalpha2 [Pichia kudriavzevii] +MQHDELLEVYIHLKDVLRQLAETPIDEELSSTDLLKDMLNIKEILESGSPLQKQSCELIRMIRRISTSIY +DMISQRSTLKTKYGLDLKSWDLDNSMMNTEDTNLTKNMDIHNSSSIDIDIDDEEISTALRDEGIHSSFCN +TDSNSIKTKDGSSRNGKRLPKETIAVLNKWFNDNIENPYVQKEDISYLKAKTNLQASQIKNWVSNKRRKR +KGSKVSPSIVEILN + +>CAO02574.1 mating type alpha2 protein [Lachancea waltii] +MGRQLVSEMLFLADAIGKKILSFKFLNSAARGNQKVSKYPHNISMHLENWFHLNSSHPYLTRKQISLLSE +RTNLSSKQIRNWMSNRRRKSKKNPIDSSLHSLLNLSSIYS + +>EDN62160.1 mating type protein alpha [Saccharomyces cerevisiae YJM789] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>QFP92360.1 mating-type protein ALPHA2 [Ogataea thermomethanolica] +MKEIDSESFSGSINDSKRLDKPMSSSPEVDFSNPLETSAISETLTLLNSALDTIVDYISTDAADSGSDEH +WGRLIYSFSKALQEHTQQSISKYDHGVGIQNVFLKTRCILYILIERKILKTKLEQLLLDHLSCLQFQRQF +GSSVMDYEISAPASFIEKPHILGFSKSSNIHSNKRFSKSSQLILEQW + +>QHB07178.1 Matalpha2 [Saccharomyces cerevisiae] +MKTYIIRLVVLRLMKSISVPRMLNRVLFMFSAILQISSMLSFLIFFDIRRCIVLLFLLEVLYSFPERTKI +KNKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRS +IENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLEN +LMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>QHB07224.1 Matalpha2 [Saccharomyces cerevisiae] +MKSISVPRMLNRVLFMFSAILQISSMLSFLIFFDIRRCIVLLFLLEVLYSFPERTKIKNKKLFSICCNLP +KLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQK +NKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIK +NWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>QHS64598.1 MTLalpha2 [Nakaseomyces glabratus] +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISLFDIHNIESGEYAYIEKVCYIIIIICHLFLVFSLSCLT + +>QHS64643.1 MATalpha2 [Nakaseomyces glabratus] +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISPFRPTLMAQNSLDT + +>QHS71949.1 Matalpha2 [Saccharomyces paradoxus] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAF1899846.1 homeodomain mating type protein alpha2, partial [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAF1907499.1 homeodomain mating type protein alpha2, partial [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAF4404995.1 homeodomain mating type protein alpha2, partial [Saccharomyces cerevisiae] +KKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIE +NDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLM +KNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>XP_033764981.1 homeodomain mating type protein alpha2 [Saccharomyces paradoxus] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAF4638090.1 homeodomain mating type protein alpha2, partial [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAF6060548.1 Mating-type-like protein ALPHA2 [Candida albicans] +MSNFPVFDPQKITNDVEANIKRMTSILKSKRLTREDEDLMKMINDCSKVLHEMLLERIMLQQDSIQFFTE +KEASDSPFSNSADTIDEDDDKKIKSRRLTKKQLLVLEGWFQKHKNHPYSQKDQTNLLIKSTKLSKSQVQN +WISNRRRKEKNTKVSPELAALLLTG + +>QNG12315.1 MTLalpha2 [Nakaseomyces glabratus] +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISLFDIHNIESGEYAYIEKVCYIIIIICHLFLVFSLSCLT + +>CAA42306.1 mating type regulatory protein, expressed copy at MAT locus [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAG2509618.1 homeodomain mating type protein alpha2 [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAG2518331.1 homeodomain mating type protein alpha2 [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAG2518428.1 homeodomain mating type protein alpha2 [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>CAF1551720.1 Matalpha2p [Saccharomyces cerevisiae PE-2] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAG8202683.1 MTLALPHA2, partial [Candida africana] +MNSHLEALFEINQRLLSHIRTMSNFPVFDPQKITNDVEANIKRMTSILKSKRLTREDEDLMKMINDCSKV +LHEMLLERIMLQQDSIQFFTEKEALDSPFSNSADTIDEDDDKKIKSRRLTKKQLLVLEGWFQKHKNHPYL +QKDQTNLLIKSTKLSKSQVQNWISNRRRKEKNTKVSPELAALLLSG + +>UCS29396.1 MTLalpha2 [Nakaseomyces glabratus] +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISLFDIHNIESGEYAYIEKVCYIIIIICHLFLVFSLSCLT + +>UCS24166.1 MTLalpha2 [Nakaseomyces glabratus] +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISLFDIHNIESGEYAYIEKVCYIIIIICHLFLVFSLSCLT + +>UCS18933.1 MTLalpha2 [Nakaseomyces glabratus] +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISLFDIHNIESGEYAYIEKVCYIIIIICHLFLVFSLSCLT + +>UCS34625.1 MTLalpha2 [Nakaseomyces glabratus] +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISLFDIHNIESGEYAYIEKVCYIIIIICHLFLVFSLSCLT + +>BDH85265.1 transcription factor [Zygosaccharomyces sp.] +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKED +QKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRS +NKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVS +ELVNGSKQGPEGSGT + +>BDH85267.1 transcription factor [Zygosaccharomyces sp.] +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKED +QKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRS +NKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVS +ELVNGSKQGPEGSGT + +>CAH2450442.1 Mating-type protein alpha2 [Komagataella phaffii CBS 7435] +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRA +IALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLS +KTQIRNWISNKRRKERSSTVSTELLQALNKSPS + +>CAX41537.1 MTL alpha 2, putative [Candida dubliniensis CD36] +MNTHSEALFQINQRLLFHIRTLSNFPVFDPQKITSDVETDIKKMVSIMKFKRLTNEDEAIMKMINDCSKV +LQEMLLERIMLQQDSIQFFTEKEASDSSFSNTVDDAIEDGDDRKTKSRRLTKKQLLVLEGWFQKHKNHPY +SQKDQTNLLIKSTKLSKSQVQNWISNRRRKEKNTKVSPELAALLLTG + +>CAI1867308.1 hypothetical protein SEUBUCD650_0C00120 [Saccharomyces eubayanus] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKKM +SEY + +>CAI1877621.1 hypothetical protein SEUBUCD650_0C01090 [Saccharomyces eubayanus] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>CAI1900938.1 hypothetical protein SEUBUCD646_0N00120 [Saccharomyces eubayanus] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>XP_002420458.1 homeobox domain-containing protein [Candida dubliniensis CD36] +MNTHSEALFQINQRLLFHIRTLSNFPVFDPQKITSDVETDIKKMVSIMKFKRLTNEDEAIMKMINDCSKV +LQEMLLERIMLQQDSIQFFTEKEASDSSFSNTVDDAIEDGDDRKTKSRRLTKKQLLVLEGWFQKHKNHPY +SQKDQTNLLIKSTKLSKSQVQNWISNRRRKEKNTKVSPELAALLLTG + +>XP_056080738.1 homeodomain mating type protein alpha2 SMKI_03G0970 [Saccharomyces mikatae IFO 1815] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>CAL1760704.1 mating-type protein Alpha2p [Maudiozyma bulderi] +MTTMNKIPIQHLLNPTSNKRLYKSDLKQINTELMYICSSLPEGIFQNIKDVKIHLHDIVTKLGIIKGQGK +LDNEERYLIKISFQLATIVANFLKSVEEEKKPSKDSKKKKKQGKSSKEEEVVFNVVTQNMMNMDNVKQNS +FRGHRFSKQNVDVLEEWYSIHKHKPYLDKKSIERLQSQTQLSRTQIKNWVSNKRRKEKTVQVSSEILQLI +NGK + +>KAL1571329.1 MTL alpha 2 [Candida albicans] +MNSHSEALFEINQRLSSHIRTMSNFPVFDPQKITNDVEANIKRMTSILKSKRLTREDEDLMKMINDCSKV +LHEMLLERIMLQQDSIQFFTEKEASDSPFSNSADTIDEDDDKKIKSRRLTKKQLLVLEGWFQKHKNHPYS +QKDQTNLLIKSTKLSKSQVQNWISNRRRKEKNTKVSPELAALLLTG + +>AAO25592.1 ALPHA2 [Nakaseomyces glabratus] +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISPFRPTLMAQNSLDT + +>AAO25604.1 ALPHA2 [Nakaseomyces delphensis] +MESKKIAITELLNPSNQENLKEKLQEINKQLISLCSSLPKRNWVPAPSSDILRSLSRTKLDSQEIRLIKT +TYRLSTLLSKLQEKDIVFNVVTKDHLLKNGTPYNPYPQPYRGHRFTKENVHTLEAWYSNHIDNPYLDPKS +LQSLAQKTNLSKIQIKNWVSNRRRKQKHPPFLLI + +>DAA07421.1 TPA: homeodomain mating type protein alpha2 [Saccharomyces cerevisiae S288C] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>DAA07518.1 TPA: homeodomain mating type protein alpha2 [Saccharomyces cerevisiae S288C] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>XP_070910655.1 homeodomain mating type protein alpha2 [Nakaseomyces bracarensis] +MSIKKITIPDLLNPSNQENLKEKLEEINKQLISLCSSLPKRNCVPAPSSDILRSLSRTKLDSQEIRLIKT +TYRLSTLLSKLQEKDVVFNVVTKDHLLRNGAPNPYAQPYRGHRFTKENVHTLEAWYSNHIDKPYLDPKSL +HSLAQKTNLSTVQIKNWVSNRRRKQTSPSPLTKI + +>CAN3503431.1 MTLalpha2 homeobox-domain protein [Diutina catenulata] +MIPTLFGNTVKSKVPPLWTMVPYRTQALASTASRRVADFVGSSLTRAFVGPTTPKTPAEQIKEFSSKIIL +LINAVCCCQAFDCDVITEMTDVATAEVAEIYRSEGSAVAADVDNFVGYVELLMYLLLCISEDFEGQTEEG +GTLIEHRTIRVLAEGEEMPEEMVSEDEDEDEDSELMDSARHDRHQLVWLREWYAMKSVQNQGNRPYLDDE +DLEILMCQTGLTDTQIRNWVSNERRRRKAAQVHPKLLETTAKFRQRRREGKICGSVANKA + +>CAN3370972.1 MTLalpha2 homeobox-domain protein [Diutina catenulata] +MIPTLFGNTVKSKVPPLWTMVPYRTQALASTASRRVADFVGSSLTRAFVGPTTPKTPAEQIKEFSSKIIL +LINAVCCCQAFDCDVITEMTDVATAEVAEIHKSEGSAVAADVDNFIGYVELLLYLLLCISEDFEGQTEEG +GTLIEHRTIRVLAEGEEMPEEMVSEDEDEDEDSELMDSARHDRHQLVWLREWYAMKSVQNQGNRPYLDDE +DLEILMCQTGLTDTQIRNWVSNERRRRKAAQVHPKLLETTAKFRQRRREGKICGSVANKA + +>CAN3361764.1 MTLalpha2 homeobox-domain protein [Diutina catenulata] +MIPTLFGNTVKSKVPPLWTMVPYRTQALASTASRRVADFVGSSLTRAFVGPTTPKTPAEQIKEFSSKIVL +LINAVCCRQSFDCDVITEMTDVATAEVAEIYKSEGSAVAADVDNFVGYVELLMYLLLCISEDFEGQTEEG +GTLFEHRTIRVLAEGEEMPEEMVSEDEDEDEDSELMDSARHDRHQLVWLREWYAMKSVQNQGNRPYLDDE +DLEILMCQTGLTDTQIRNWVSNERRRRKAAQVHQSFWKLPRNFVKGVARGRSVEAWPTRHKLGEAPVARL +S + +>CAN3475760.1 MTLalpha2 homeobox-domain protein [Diutina catenulata] +MIPTLFGNTVKSKVPPLWTMVPYRTQALASTASRRVADFVGSSLTRAFVGPTSPKTPAEQIKEFSSKIIL +LINAVCCCQSFDCDVITEMTDVATAEVAEIYRSEGSAVAADVDNFVGYVELLMYLLLCISEDFEGQTEEG +GTLIEHRTIRVLAEGEEMPEEMVSEDEDEDEDSELMDSARHDRHQLVWLREWYAMKSVQNQGNRPYLDDE +DLEILMCQTGLTDTQIRNWVSNERRRRKAAQVHPKLLETTAKFRQRRREGKICGSVANKT + +>CAO2664650.1 MTLalpha2 homeobox-domain protein [Diutina rugosa] +MALPKFDFGPQAPLTPQQSYHQLASQARILINYVIGRRTFNCDEIAAQASEINQALKDLRSKYPHSFTAE +YEAVVDSLHLLLGALKIRLDVLQQPSTAKFKEHSKLKQHSNITFVGSTNSMRRRNTRHEPTQVVFLRHWY +EMRSAKDGNQPVYLRDPDDLDYLIQKTRLTETQIRDWVSNERRRRKILGRHHHHRYELPNEW + +>YCL21937.1 Mating-type protein alpha2 [Komagataella phaffii] +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRA +IALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLS +KTQIRNWISNKRRKERSSTVSTELLQALNKSPS + +>CCA89264.1 mating-type alpha2 protein [Kluyveromyces marxianus] +MSKIPVQSLLNPESPREHFYKTYEIEHKQSQVDRKIQNLLTEFKRVFQLVLLSREVFIQPNYVEIDEFVK +TIRQTLIYGSGEGELNTIISRNIFNSVLCLLIVLQEKQKISLLENNILSKVESFKERRSENTNTVEFRIL +SSEHLNPTLNKVGKKNRFPKKSIKLLENWYECNRNSPYLTSNHLDYLKCNTGLNKTQIKNWVANRRRKDK +NSRVSLDIKNIFRQQ + +>KAN7452647.1 homeodomain mating type protein alpha2, partial [Saccharomyces cerevisiae] +NKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSI +ENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENL +MRNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>KAN7457998.1 homeodomain mating type protein alpha2 [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKIRDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>sp|P0CY08.1|MTAL2_YEAST RecName: Full=Mating-type protein ALPHA2; Short=MATalpha2 protein; AltName: Full=Alpha-2 repressor +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>XP_003685884.1 homeodomain mating type protein alpha2 TPHA_0E03610 [Tetrapisispora phaffii CBS 4417] +MNKIPINKLLNPSQHYNLTEKLQQINITLSNLCTKLPDTITDLTEADHRELQDILLYLTTVVKQQELKKE +EIMLVKTTYQLCTTLTLMVKSCKQENDKENKENEIIKYESGTSDNSETNSSCNSSDNEDKNSILVFNVIT +QDMMNNNKKNNKSYRGHRLPKKNVQYLEDWYMDHRKNPYLNEINIKLLMSKTSLSRIQVKNWISNRRRKE +KSITISPEVSALLRDNKTITS + +>CAE84417.1 MATalpha2 transcription factor [Ogataea angusta] +MDDMLTKIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKLCKSSMEGFTTERSLKYTRDCPQYAA +VTTSQPRYRIEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERT +LTISKELSDLLNQDTRCSPNDVV + +>XP_003956854.1 homeodomain mating type protein alpha2 KAFR_0D00720 [Kazachstania africana CBS 2517] +MNKIQIQDLLNSPRDVDTKQELRDINDKLFALCSKLPSSEALLEAEFHLELRDIMLNLSKLQDTSRMGSE +EKHLAYTAHQLTCTMITLIKGMKNFHGTGGHSYSSSKSESDDKTVFNVVTQDMMNLNRTGISYRGHRFSK +ENVKVLERWYTAHIDRPYLNRQSTEYLISKTGLSRVQIKNWVSNRRRKEKSVHVSPELIQLLQKKS + +>BAM78606.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAM78611.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAM78613.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAM78622.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAM78625.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAM78628.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAM78631.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAM78633.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAM78637.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>BAM78639.1 alpha2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>CAA45335.1 MAT alpha2 [Saccharomyces cerevisiae] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>XP_445025.1 homeobox domain-containing protein [Nakaseomyces glabratus] +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISPFRPTLMAQNSLDT + +>CDM87332.1 hypothetical protein [Zygosaccharomyces sapae] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRP +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>CDM87335.1 hypothetical protein [Zygosaccharomyces sapae] +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKED +QKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRS +NKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVS +ELVNGSKQGPEGSGT + +>CDM87338.1 hypothetical protein [Zygosaccharomyces sapae] +MNKISIEQLLNPIHQEFPHEKLKEINDKLLTLCSKLPAENEKLEEDRSELEKILPSLNTLLKEKPSSKDQ +KMIQKIHQLFSVLSALLNEVSELRSKKNKLFPSKQMSRLSNETTLGDKGKNRTVFNVVTQDTLEYTRNSR +KTYRGHRLPKYVTNSLESWFKRNIKHPYLNNSSIKQLITETKLSGPQIKNWVSNRRRKEKSLTVSFDVSE +LVRKSKRDREDSSG + +>CDM87341.1 hypothetical protein [Zygosaccharomyces sapae] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRP +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>CDM87344.1 hypothetical protein [Zygosaccharomyces sapae] +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKED +QKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRS +NKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVS +ELVNGSKQGPEGSGT + +>CDM87347.1 hypothetical protein [Zygosaccharomyces sapae] +MNKISIEQLLNPIHQEFPHEKLKEINDKLLTLCSKLPAENEKLEEDRSELEKILPSLNTLLKEKPSSKDQ +KMIQKIHQLFSVLSALLNEVSELRSKKNKLFPSKQMSRLSNETTLGDKGKNRTVFNVVTQDTLEYTRNSR +KTYRGHRLPKYVTNSLESWFKRNIKHPYLNNSSIKQLITETKLSGPQIKNWVSNRRRKEKSLTVSFDVSE +LVRKSKRDREDSSG + +>CDM87350.1 hypothetical protein [Zygosaccharomyces sapae] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFLRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRP +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>AHY79718.1 Matalpha2p [Saccharomyces cerevisiae YJM993] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>sp|Q707Y8.1|MTAL2_PICAN RecName: Full=Mating-type protein ALPHA2; AltName: Full=MATalpha2 transcription factor +MDDMLTKIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKLCKSSMEGFTTERSLKYTRDCPQYAA +VTTSQPRYRIEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERT +LTISKELSDLLNQDTRCSPNDVV + +>sp|Q86Z42.1|MTAL2_CANGA RecName: Full=Mating-type-like protein ALPHA2; AltName: Full=MTL1alpha2 protein +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIK +TTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNS +QQYLAQKTNLSKIQIKNWVANRRRKQKSIYISPFRPTLMAQNSLDT + +>sp|Q874N1.1|MTAL2_NAKDE RecName: Full=Mating-type protein ALPHA2 +MESKKIAITELLNPSNQENLKEKLQEINKQLISLCSSLPKRNWVPAPSSDILRSLSRTKLDSQEIRLIKT +TYRLSTLLSKLQEKDIVFNVVTKDHLLKNGTPYNPYPQPYRGHRFTKENVHTLEAWYSNHIDNPYLDPKS +LQSLAQKTNLSKIQIKNWVSNRRRKQKHPPFLLI + +>sp|Q9HDS5.1|MTAL2_KLULA RecName: Full=Mating-type protein ALPHA2; Short=MATalpha2 protein +MSRIPIHSLLNPSESCKSISNVPSNYRDLSTFNKERAKVITTFQEMFYSMLENNDDYNKIESLIRNFQPK +LTWSHKCESLTFKQKAYLTAIIQKSIKSLLVLLKEKGKMREIEFSRKEVRKINKYRQSSKNFESVNIKIL +TQDLMHSNNNEFKKGKRFLKSHIQLLENWYSMNRRNPYLAENDLAYISKNTTLTKTQIKNWLANRRRKDK +ITEVSSDIRNILN + +>sp|Q9UW22.1|MTAL2_CANAL RecName: Full=Mating-type-like protein ALPHA2; Short=MTLalpha2 protein +MNSHSEALFEINQRLSSHIRTMSNFPVFDPQKITNDVEANIKRMTSILKSKRLTREDEDLMKMINDCSKV +LHEMLLERIMLQQDSIQFFTEKEASDSPFSNSADTIDEDDDKKIKSRRLTKKQLLVLEGWFQKHKNHPYS +QKDQTNLLIKSTKLSKSQVQNWISNRRRKEKNTKVSPELAALLLTG + +>NP_009866.1 homeodomain mating type protein alpha2 [Saccharomyces cerevisiae S288C] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>NP_009868.3 homeodomain mating type protein alpha2 [Saccharomyces cerevisiae S288C] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJP37469.1 Matalpha2p [Saccharomyces cerevisiae YJM1078] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ31954.1 Matalpha2p [Saccharomyces cerevisiae YJM1356] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ32100.1 Matalpha2p [Saccharomyces cerevisiae YJM1381] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ32244.1 Matalpha2p [Saccharomyces cerevisiae YJM1383] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ32390.1 Matalpha2p [Saccharomyces cerevisiae YJM1385] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ32538.1 Matalpha2p [Saccharomyces cerevisiae YJM1386] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ32684.1 Matalpha2p [Saccharomyces cerevisiae YJM1387] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ32831.1 Matalpha2p [Saccharomyces cerevisiae YJM1388] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ32974.1 Matalpha2p [Saccharomyces cerevisiae YJM1389] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ33117.1 Matalpha2p [Saccharomyces cerevisiae YJM1399] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ33262.1 Matalpha2p [Saccharomyces cerevisiae YJM1400] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ33408.1 Matalpha2p [Saccharomyces cerevisiae YJM1401] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ33556.1 Matalpha2p [Saccharomyces cerevisiae YJM1402] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ33700.1 Matalpha2p [Saccharomyces cerevisiae YJM1415] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ33839.1 Matalpha2p [Saccharomyces cerevisiae YJM1417] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ33989.1 Matalpha2p [Saccharomyces cerevisiae YJM1418] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ34135.1 Matalpha2p [Saccharomyces cerevisiae YJM1419] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ34282.1 Matalpha2p [Saccharomyces cerevisiae YJM1433] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ34432.1 Matalpha2p [Saccharomyces cerevisiae YJM1434] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ34579.1 Matalpha2p [Saccharomyces cerevisiae YJM1439] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ34729.1 Matalpha2p [Saccharomyces cerevisiae YJM1443] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ34875.1 Matalpha2p [Saccharomyces cerevisiae YJM1444] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ35020.1 Matalpha2p [Saccharomyces cerevisiae YJM1447] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ35163.1 Matalpha2p [Saccharomyces cerevisiae YJM1450] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ35312.1 Matalpha2p [Saccharomyces cerevisiae YJM451] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ35458.1 Matalpha2p [Saccharomyces cerevisiae YJM453] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ35602.1 Matalpha2p [Saccharomyces cerevisiae YJM456] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ35747.1 Matalpha2p [Saccharomyces cerevisiae YJM470] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ35892.1 Matalpha2p [Saccharomyces cerevisiae YJM541] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ36039.1 Matalpha2p [Saccharomyces cerevisiae YJM554] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ36183.1 Matalpha2p [Saccharomyces cerevisiae YJM555] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ36330.1 Matalpha2p [Saccharomyces cerevisiae YJM627] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ36472.1 Matalpha2p [Saccharomyces cerevisiae YJM681] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ36619.1 Matalpha2p [Saccharomyces cerevisiae YJM682] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ36766.1 Matalpha2p [Saccharomyces cerevisiae YJM683] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ36912.1 Matalpha2p [Saccharomyces cerevisiae YJM689] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ37059.1 Matalpha2p [Saccharomyces cerevisiae YJM693] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ37202.1 Matalpha2p [Saccharomyces cerevisiae YJM969] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ37351.1 Matalpha2p [Saccharomyces cerevisiae YJM972] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ37500.1 Matalpha2p [Saccharomyces cerevisiae YJM975] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ37640.1 Matalpha2p [Saccharomyces cerevisiae YJM978] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ37778.1 Matalpha2p [Saccharomyces cerevisiae YJM981] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ37927.1 Matalpha2p [Saccharomyces cerevisiae YJM984] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ38076.1 Matalpha2p [Saccharomyces cerevisiae YJM987] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ38225.1 Matalpha2p [Saccharomyces cerevisiae YJM990] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ38374.1 Matalpha2p [Saccharomyces cerevisiae YJM996] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ38520.1 Matalpha2p [Saccharomyces cerevisiae YJM1083] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ38665.1 Matalpha2p [Saccharomyces cerevisiae YJM1129] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSSYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ38815.1 Matalpha2p [Saccharomyces cerevisiae YJM1133] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ38953.1 Matalpha2p [Saccharomyces cerevisiae YJM1190] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ39096.1 Matalpha2p [Saccharomyces cerevisiae YJM1199] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ39245.1 Matalpha2p [Saccharomyces cerevisiae YJM1202] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ39393.1 Matalpha2p [Saccharomyces cerevisiae YJM1208] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ39526.1 Matalpha2p [Saccharomyces cerevisiae YJM1242] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ39674.1 Matalpha2p [Saccharomyces cerevisiae YJM1244] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ39821.1 Matalpha2p [Saccharomyces cerevisiae YJM1248] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ39971.1 Matalpha2p [Saccharomyces cerevisiae YJM1250] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ40119.1 Matalpha2p [Saccharomyces cerevisiae YJM1252] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ40265.1 Matalpha2p [Saccharomyces cerevisiae YJM1273] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ40407.1 Matalpha2p [Saccharomyces cerevisiae YJM1304] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ40551.1 Matalpha2p [Saccharomyces cerevisiae YJM1307] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ40695.1 Matalpha2p [Saccharomyces cerevisiae YJM1311] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ40834.1 Matalpha2p [Saccharomyces cerevisiae YJM1326] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ40982.1 Matalpha2p [Saccharomyces cerevisiae YJM1332] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ41129.1 Matalpha2p [Saccharomyces cerevisiae YJM1336] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ41274.1 Matalpha2p [Saccharomyces cerevisiae YJM1338] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ41417.1 Matalpha2p [Saccharomyces cerevisiae YJM1341] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ41567.1 Matalpha2p [Saccharomyces cerevisiae YJM1342] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ41710.1 Matalpha2p [Saccharomyces cerevisiae YJM1355] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ41856.1 Matalpha2p [Saccharomyces cerevisiae YJM1460] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ42000.1 Matalpha2p [Saccharomyces cerevisiae YJM1463] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ42135.1 Matalpha2p [Saccharomyces cerevisiae YJM1477] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ42276.1 Matalpha2p [Saccharomyces cerevisiae YJM1478] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ42420.1 Matalpha2p [Saccharomyces cerevisiae YJM1479] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ42565.1 Matalpha2p [Saccharomyces cerevisiae YJM1526] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ42714.1 Matalpha2p [Saccharomyces cerevisiae YJM1527] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ42854.1 Matalpha2p [Saccharomyces cerevisiae YJM1549] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ43001.1 Matalpha2p [Saccharomyces cerevisiae YJM1573] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ43145.1 Matalpha2p [Saccharomyces cerevisiae YJM1574] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ43291.1 Matalpha2p [Saccharomyces cerevisiae YJM1592] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ43434.1 Matalpha2p [Saccharomyces cerevisiae YJM1615] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ43577.1 Matalpha2p [Saccharomyces cerevisiae YJM189] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ43722.1 Matalpha2p [Saccharomyces cerevisiae YJM193] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ43865.1 Matalpha2p [Saccharomyces cerevisiae YJM195] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ44003.1 Matalpha2p [Saccharomyces cerevisiae YJM244] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ44149.1 Matalpha2p [Saccharomyces cerevisiae YJM248] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ44292.1 Matalpha2p [Saccharomyces cerevisiae YJM270] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ44440.1 Matalpha2p [Saccharomyces cerevisiae YJM271] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ44580.1 Matalpha2p [Saccharomyces cerevisiae YJM320] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ44728.1 Matalpha2p [Saccharomyces cerevisiae YJM326] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ44874.1 Matalpha2p [Saccharomyces cerevisiae YJM428] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AJQ45020.1 Matalpha2p [Saccharomyces cerevisiae YJM450] +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDE +EKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKEN +VRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE + +>AMK38004.1 alpha2 copy 2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKED +QKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRS +NKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVS +ELVNGSKQGPEGSGT + +>AMK38007.1 alpha2 copy 1 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIERLLNPVHQEFPHEKLKNINNKLLALCSKLPSENEQFEKDKAELEKILPSLNILIRECGSSKED +QKMVHRIHQLSSVFSLLLNEVSELKSKRSQFRRSKPKYHLPYAAALGNTGKEGMVFNIVTQDTLNSARRS +NKTYRGHRLPKHITRLLESWFNRNIEHPYLQTSSMKELMVETKLSGPQIKNWVSNRRRKEKSLTISFEVS +ELVKESKQGTEELNE + +>AMK38010.1 alpha2 copy 2 transcriptional factor [Zygosaccharomyces rouxii] +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKED +QKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRS +NKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVS +ELVNGSKQGPEGSGT + diff --git a/results/2026-10-30_mat_hmms/data/public_ids.json b/results/2026-10-30_mat_hmms/data/public_ids.json new file mode 100644 index 00000000..0fe353ed --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/public_ids.json @@ -0,0 +1 @@ +{"a1": ["10643642", "1064980705", "1078728146", "1111957197", "1147427245", "1168016802", "1168016806", "1168016815", "1208526", "1255179393", "1255182381", "1275605776", "1310757", "1431025129", "1436831057", "1436831060", "149941279", "150866660", "1777440416", "1777441388", "1907235", "2221828065", "2233324664", "2272020016", "2290183102", "2290214277", "2290214325", "2325077603", "239835855", "255723658", "2670939598", "2696391868", "2725026953", "2725185781", "2814993878", "2814998884", "294657841", "2978910883", "3107252231", "3150686625", "324975486", "324975494", "324975501", "324975510", "324975519", "328353785", "332594836", "338818276", "353526254", "39722363", "39722372", "39722376", "39722385", "39722389", "4138702", "448261550", "448261560", "448261565", "448531612", "574139969", "574141524", "5771503", "585865273", "598068887", "60390191", "60390192", "60390194", "60390216", "998247169", "998247172"], "alpha2": ["1000052", "1000053", "10643638", "1069876559", "1073751006", "1168016797", "1168016808", "1168016811", "1188069868", "1238273017", "1275605769", "1275605772", "1275605778", "1310758", "1436831059", "1442830839", "149941272", "151943860", "1764573505", "1789108160", "1789108206", "1804577421", "1804577466", "1804585364", "1806626129", "1806633809", "1835438291", "1835457366", "1842038706", "1883956896", "1891256536", "1907181", "1980427758", "1980438166", "1980438263", "1997627859", "2073132647", "2112344291", "2112350050", "2112357490", "2112363463", "2221828056", "2221828060", "2233324738", "223643800", "2290182956", "2290183053", "2290214176", "241955475", "2507684422", "2727556244", "2790807706", "27948802", "27948816", "285810637", "285810734", "2897769334", "3077267469", "3077276934", "3077280882", "3077313136", "3101722226", "3150686554", "332594849", "3327663429", "3327669489", "338818297", "367002299", "39722383", "410078546", "448261545", "448261552", "448261555", "448261567", "448261571", "448261575", "448261579", "448261582", "448261587", "448261590", "499332", "50285193", "585865245", "585865249", "585865253", "585865257", "585865261", "585865265", "585865269", "628270573", "62900610", "62900613", "62900615", "62900621", "62900626", "6319785", "63536787", "761380733", "763825553", "763825700", "763825845", "763825992", "763826141", "763826288", "763826493", "763826637", "763827183", "763827330", "763827634", "763827783", "763827983", "763828123", "763828309", "763834117", "763837467", "763837618", "763867333", "763867484", "763867631", "763867777", "763867997", "763868233", "763869136", "763869281", "763869433", "763871815", "763885670", "763906252", "763906400", "763906546", "763906820", "763907002", "763907149", "763907297", "763907442", "763907592", "763907751", "763908186", "763908325", "763908475", "763908625", "763909533", "763909689", "763913728", "763916038", "763916189", "763916328", "763916472", "763916622", "763916771", "763916905", "763967571", "763967719", "763967870", "763971155", "763971302", "763971445", "763971590", "763971735", "763971875", "763972024", "763972172", "763972318", "763972462", "763972840", "763976238", "763993952", "763997193", "763997329", "763997471", "764005496", "764005642", "764005792", "764005933", "764006081", "764013790", "764016446", "764019715", "764019859", "764020005", "764020256", "764020395", "764020542", "764020686", "764020835", "764020976", "764021125", "764021272", "764021419", "998247175", "998247179", "998247183"], "a2": ["1073750995", "1078728147", "1111957199", "1255179395", "1255182379", "1275605775", "1444873513", "149387917", "149941278", "150866661", "1777440415", "1777441387", "1838471131", "1919694792", "2221828054", "2221828058", "2221828062", "2221828064", "2233324663", "2325079067", "2670939603", "2696391867", "2725026960", "2725185777", "2797499328", "294657843", "2978910882", "3101721909", "3107252232", "3150686626", "324975483", "324975493", "324975498", "324975509", "324975516", "332594837", "338818277", "39722375", "39722390", "4138703", "448531608", "47008825", "574139970", "574141523", "60390162", "60390196", "60390217", "63101124"]} \ No newline at end of file diff --git a/results/2026-10-30_mat_hmms/data/set_a1.faa b/results/2026-10-30_mat_hmms/data/set_a1.faa new file mode 100644 index 00000000..aea3b445 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/set_a1.faa @@ -0,0 +1,58 @@ +>5476_sc5314_MTL_A|MTLA1 +MNSEIESSLTLLKSVEKLVQATSVYKNEDNEEIFLQLKRERQENSNSHEETETFFHESLDRLMKLSSIAGSKVQYELSLNNLYNLLLRTRKQEEEEAFIFPTVSTEEFALEQITFEVSDQMDDDKDSEDDILIKGEEIKKSKKKRQRLDNSTKEFLEKVFEKNKQPNRRERELIAEKHGVSLSQIRVWFTNKRMRKKEPKSKAKSSSNST +>CAK7898777.1|MATa1_protein +MRRLLILSQSLDMNPNLLHFNIRNLYDTLAKNNIKKPIVHTLNVYGEDCNFLLESVRMSVSTGKPIKKKFTQEYKTKIDGTPSEPTYLKKKRTVIPQISRIYLENFFKVNPTPDRNERIMISEKTNLTYDQVRIWFNNKRMRSKGFNFVLHSIKPGSSLRD +>XP_002546761.1|Mtla1p +MLKAQKEEEEILTVTEEGGDEFKLAKITISVSDQADDFRASDKESLSSEEEGKKLKKKRQRLDNTTKEFLEKVFERKSQPNRRERELIAEKYGISLSQIRVWVCIIFYYFQFILSELLTNITSKFTNKRMRKKKPDSSKKESDKTDDT +>498019_b11205_MTL_A|MTLA1 +MDQKPSKFNELFQTVSTLEQLTSFSKKKWKIPLAQDNYVDSMKTLTEEAGFGKSFHKAIEQVYLWKKVTKNFENEESSLTLTSLCEATSVTKNENASCKRRRSHLTMGIKEYLEQVFKKKPHPTRSEYEVIARSCNLSSRQVRVWFTNKRYRSKPRGNLKEEDEDR +>36911_cl143_MTL_A|MTLA1 +MILSDGKNDLQHFLTEVRALTSAYVTDELPDHNRIESQSFIERMGHLVNNIFMKINVFTILKSLQMSLIYEHKMRKAEVKNLDQKFYLRRTRVKFFKREASKAVRKSFTSEAKTKLELFYLANTNPRKAEIVKMARECNMPEEKVRAWFANKRYRERTQNKCIQ +>4959_cbs767_MTL_A|MTLA1 +MEGYTKSHDVVVEVPENENIKAYTTRVESGHISEPSSSNGSNLTLNVKNEYENISSDGKVIDRYQLLKEKGYSHGFLLEEASISVTCETPDPHLQQQRKVSRFGREVHENTNVFPNKKGSYKCDSEASNRFLENVFKTNSYPTSKERHIIAKKCGLSPTQVGAWFTNRRNVSE +>XP_020074339.1|Mtla1p +MKVDNYQPKQDIESRLCQLNGILTHLKIHITNVCTLPDFQPESMQKDLTGDCLVVRLLLDYNIQLSESEQALLAKINKLSNLLILMIEERQRIRIIFDRTPRTLQAQTVVEEANKISDASDKKISFEVLTQSDISKGPKTNRLQDHQVLILNEWYGRNKKHPYVDENSLKYLIEKTRLSETQVRNWFSNKRRKHRSNKVSHELEPLLS +>28985_nrrl-y-1140_MATsc_MATa|MATA1 +MCDNDMADIQSKLSSFCEEIRALALKEGYNLEGDKSPSSKPYFMSWPKEIDVNHPNFAFFTKLQFQYDKSLETILNSCYLQELQLDPTRIIETLQQHFNNSILRYADIENISDVKDESPMSFDTEHECTDTSEDISDKSEISSTNSDNPIQNCYPTYKRSFIKHESRGILEKIFKVKQCPNTSERLYIAQKLDLTPSQVRIWFTNKRMRAKKHTTGKGTKRKSKKYPS +>XP_022675170.1|mating-type_protein_A1 +MDGHDKKNPILLLKELCQEAQGLAGVQLKEDGFSSPFKTFSIPFPEEAVKKCSNKALLLQLYHQYSTSLETILNKCPLEDLKVDPQMVADAFNNQFLRTIQLLPVENNLKIKEQNPSDSSGKEDSKNDTVEEDSPSPDNQNSRTAKKFFLEPEAKDLLEKVFKVKKCPNTSERLFIAQKLGLTASQVRIWFTNKRMRTKSKQKKVKPIAGPIK +>460519_cbs-7435_MATkp_MATa|MATa1 +MSGNTFREITAACLLSMILSTDDYTKPATSCHMHQLTAPDLSDYSLLDALFVTCDRALEYQALTYRLQASMEDAMKIIESARYQTKDLPTLDNIFKTFYKTLLDTIESIEATGTSTLLSDFTHQISFFNIDPSVISLSDETKECAYSFFSDSLHHHPVKRTRKTISKEVKKILEEAFKAKAAPNTKERLLIAQRCALTPLQVRMWFTNKRTRNKIKQRLQAQSHP +>KAM3161348.1|Mating_type_a1_protein +MDSRGLIISLESIKKVCLMLLEPNYKSRQEVCDKIYENYATLLQDAVVESKEKPRSGQQRRTLLSKDTRLFLESVFEKKRSPNSRERKAIADKCGLTPIYISFSVFSSSLGHGSQQKTSPTYSPTFLMFFMLWSNFRTFNTP +>CAO02580.1|mating_type_a1_protein +MSQLPSFLPDIFQKLSRMKEISHVKKMDTSSILITLESIKKACGALLEPDVERRQEVCDKITVGQRRTLFSKDTRLFLESVFEKKKSPNSRERKAIADKCGLTPVQVRVWFTNRRWR +>XP_003870289.1|Mtla1p +MNSEYHKGKALLDSLTVLEELTKTTTLIGPTKEQGNSDTTDNPHQLLQDLKENWNSIDPRVEWGLKQLPSNLRTIERFEQEDMVSIPLQSNAKFLLRRVSISVRNSTTKKLTLHDTYLCGSWNALKIPRQRLSLASKELLERIFLVKQSPNREERGLIAEKCGVSPLQIRVWFTNKRMRSKRQ +>XP_051608499.1|Mtla1p +MNSEYYKGKALLISLSALEDITKSTSSTNLSHSSPNLDVDNGETSQTPDKILEDIKSSCGPIDPRLEWGLSQLPDTLRRIEQFESENRTNIPLQHNSNFFLQRVAISIGGTVRSKHTQETDLCGSWGGLRRSRKRLSSSSKELLEKIFLVKQSPNRRERELIAGKCGVTPLQIRVWVCTRSNPICQEATLKIKVLT +>CAL9736377.1|mating-type_protein_A1 +MQQLLNVSSKLILESDLCLCERKNLLLQIYDNSSGRTHLTKDTRLFLEKVFIQKQWLTKDERQLIATKCGLSPLQVRIWFIKCRHKLI +>CAL9731627.1|mating-type_protein_A1 +MQRSFNVSTKLILESDLCLCERKNLLLQMYDNSSGRTHLTKDTRLFLEKVFIQKQWLTKDERQLIARKCGLSPLQVRIWFINKRYRNK +>CAE84400.1|MATa1_protein +MNVQEIHNIREACITILSGTKHNSVLFEPCDKFDEVINSLDIDPDSKETLRRSVSLLVGTRNHKRGCNIDKKTKDMLNKVYEQKQYLTKEEREFVAKKCNLTPLQVRVWFANKRIRNKNTKM +>CAE84419.1|MATa1_transcription_factor +MQFTILNEPSLDSQRREGDLASENYVFGDIRKEGVRILEDSLRSERNALLAIGAGGAADRVKQHYRKLLDDWEAAIMEIRSNDKYESLCCGFAIEKAEVETEMRNTEELDVYKECLVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>1005962_dl-1_MATog_MATa-MATalpha|MATa1 +MQFIILNEPSLDSQRREGNLASENCVFARDIRKEGVKVLEDSLRSERNALLAIGAGGAVDRVERHYRRLLDGWEAAIMEIRSNDKYESLWRGFAIEKAEVETEMRKVEKQWPGELDVYKECFVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>460523_cbs-4732_MATog_MATa-MATalpha|MATa1 +MQFTILNEPSLDSQRREGDLASENYVFGDIRKEGVRILEDSLRSERNALLAIGAGGAADRVKQHYRKLLDDWEAAIMEIRSNDKYESLCCGFAIEKAEVETEMRNTEELDVYKECLVSECFDKKKRRHIPESSKELLEKAFKVKRFPNSKERERIARECGISPLQVRVWFTNKRARSKSRA +>4909_cbs-573_MATpk_MATa|MATa1 +MYEMSLGKDVGTIILPILTICSLTSEFCDKNETRKPLNIELPRLESMSGIPTTENSNLRRLIDDCQDSFEKEVGILMNNDLPLTLVEDLFKRYKEFFWEKYKLMNNLIDTPRLPEHLTFDDHWILDDSLSLENLNDIPMLQPYQLSDFEITQAEITTTSDILGEPLTIELSGSQLSEQNTPIFSQNFGIYDYLNSITNEIESSTTDSGHNRRHRPSKDVKEKLEQIFTKVKNPNSVEREYIAKKCNMTPTQVRIWFTNKRARQIKTRDT +>XP_062812646.1|Mtla1p +MNSELLKCKPLIENLCALESLTRRFEDTKLDDYQCLEEELSCSTDGTLTNIIGNSRIRALNLSKQFGLGDKFSIINLYDSMRSYVLENTLPQRIIDRSERTSFTLEKVTVKITNQEGGLKAESQGVTGGSTRKRTYINNEIRRFLEKAFHKKRNPTRSDRAAIAQKCGLTPLQVRVWFTNKRMRSKSK +>4932_s288c_MATsc_HMRa|MATA1 +MDDICSMAENINRTLFNILGTEIDEINLNTNNLYNFIMESNLTKVEQHTLHKNISNNRLEIYHHIKKEKSPKGKSSISPQARAFLEQVFRRKQSLNSKEKEEVAKKCGITPLQVRVWFINKRMRSK +>XP_064741610.1|Mtla1p +MDSELASCSRLLEDLLLLDLLTRDSSESSEQIGVDSSERSTSGNVFEESADRMIKYASQVGNGGGEYSFCIRNLYDTLKKESLKTSESIATFIDTADDFHLTKAVITVSNKRNDSFDEEQIISIKNNFMNVKRRRCILSRTTREFLQKAFEAQPFPNRKDRELIAKKCGISNLQVRVWFTNRRMRSKRRN +>XP_064762305.1|Mtla1p +MYKLSKLVSDSEEYAFCIRNLYDALEKQSIGNKIYITEHDDRSEGFYLSKATISVSNKKTEPKSNYILDSIRSSYRASRRKRCILTAETKEFLQKAFDAQPFPNRKDRELLAKKCGLTNLQVRVWVSNSL +>XP_001386328.2|Mtla1p +MLRDGECFRYLDSSAVLKYSIQNLFDNAVEKASEKKRTFLDHDAQPQEFHLRSVMISITCSTNLSSKVALGDKALVRKRRRKIHSPEIRAILEKEFSLDPSPSRAHRKSIAVRCNMSTSEVRIWFANRRNRFKRYQ +>4952_w29_MATyl_A|mata1 +MPSRTPTDIWRCQRLILAARKGETTCQALHEQSIEISSSLKWFEEISEWMSKFPESGLYISTTIWTFLEGVPNEHRIKYDRLIRHYITYIQQLVPARLVTAYLDSLFKICSHPTRTERRIIGEHCGISLHQVTQWFTNRRFREPKTDSPDDGSTRFIPSPMTSPGSIDECSFPESP +>BAX02937.1|a1_transcriptional_factor +MSDKTFNSPNDLQKQYLNSFHKVFKTISNSSLSTQEKGVLIRCISQNYSSSFRMTISSLANEYCGDQYIFNKDSIKRSYSERVLGKDCKGKNRAFSKESRKFLESVFEKKKTLTPREREIVGASCGLTPLQVRIWFNNKRMRSK +>BBB04551.1|mating_type_a1 +MSDETFNSLNDLQEYYLSSFHKVFKTINNTSLSIQEKNVLIRCVSRNYSNSFHVTLSSLVNEYSGDQYIFEGNSSKNSSSKEVSMRNCKGKNKAFSKESRNFLERVFERKRTLNSREREAVAANCGLTPIQVRIWFNNRRMRSK diff --git a/results/2026-10-30_mat_hmms/data/set_a2.faa b/results/2026-10-30_mat_hmms/data/set_a2.faa new file mode 100644 index 00000000..f8187ea7 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/set_a2.faa @@ -0,0 +1,28 @@ +>5476_sc5314_MTL_A|MTLA2 +MPYTFNFPKSQSSFKYSIKSLTNSRSFPMISIYVTPQSTPKFINNQSIDKMTVLSSFRSRNSFIIARSILSKLLRKNKADFKKVSKSVSLLWGSVDNSFKNYFEYLSILESQWHDKKSSNLNPISSTRTTSELNYIELCSSIYNQSRKHPRSVQGNKAKFRLYKNVSKKSNRKPRRLKNGFKVSRSTINCSYGIYTEDVFQ +>CAK7898773.1|MATa2_protein +MEFVNQDIYCRHLDSTTPHSVYKTFFPPLIEKSTTHTISSISNSTSFPLIATSTRIKNLQPDDNFRVYTSFPCRKPNKVKKDSAEQNYTGGRNKFIIARNLLSQLVRDQGLISRRDVSKTTSLVWMSGSSQFQSYFAYLSILDTSWNEFKSILQRCRTAEAVENIEWKGVLSHNSAFCSYTKDKVALFKRETPNVSFKVVRRKFDITKPKLRKKSKRINQDFLSNSCINFIEDIYK +>498019_b11243_MTL_A|MTLA2 +MIYSTTSRLPAKSKGPLSKRTRCGHFPYSELKFLHTSTEATDKSKRYRPRNQFMMARQHLTSTFKDGRCARVKSIRASQVCSYYCYLAGLAAAYYETSSAQNSTASFQCNTARLHHVNSAFKVVKFRVQLKQRVPRKEKSFFGLQVEDVVPS +>36911_cl143_MTL_A|MTLA2 +MTAEEPLYSRTFSLPSKRKFNKQPTNAKRFPLRATNLISKAPQVDEEFLKRYRSRNQFIMARQLISPFLKGVHNSASRSKLVSCIWKTKTSQFEKYFRYLAALDSNYFEELIKFRSANNAFVGDSYRSMCSSSYSLKRTYRDAKFKVHKFKTEIKRSFIVKQNEYFFGLQVEDIMG +>4959_cbs767_MTL_A|MTLA2 +MSLSPDKIFTFCFKLPRRIDIPVESNQPTTKKNFKIKELTTTNSYPSISINIKQYVSFTSEKYIEASRKPTAKDKKTAITRGSFRTRNKFIIARNLLCRLVKHEKALSSTEISKVVSLVWKESNKSLHDYFEYLSLLETYWYDKLMSSYGNSTLQATATAAVSIRLSSVPYENLCASCYNKHKQKKTLNYDYSKSAKFRVHKDFKEKKKTFLTIKDHSKKLEKLSDAFRINQCTITTNFGIVTEDMFFNSL +>28985_nrrl-y-1140_MATsc_MATa|MATA2 +MANSTLRRTTFFKLTTTEDEDTIPKLLQPNNNSVAFPNLKRTGKAFTNDTIKENTKKYTRPRNQFVLMRTLFNRRVNNHILQYYNKSKLEKKMFTLTSKITSELWNESSPDLKSYFSLLATLEENWHKYTHYCSWDRNSAQTLSMEPIELSQVRPRLISSLTVGAGSSVSTYTLRELLLVRKIKSRKRKTTTLTQDSSPTTKFKYKFKKQPKTKMNSNLSKLRFKSKQPPTPPEENSNVFKKRYTSENRIIEDLFLM +>XP_022673680.1|mating-type_protein_A2 +MSNSTLRRTTFFKLSTKEEACSSNKAVQSNNNSIEFPNLKRNGKAFTCSSNEKTSKKFTRPRNQFVLMRTLFNRCVTTHILEHFNQTKIQKNMFSVTSKITSELWNESPSELKVYFLLLATLEENWHKNKHYCSWDKQSSQTLSMEPIENSHIFSRLAISLSMGVGSAVSSYSAKDLCLFPKTRTKKKRVASPTLLPNQRFNSKFNSKLSQDTKTKSPKSLVRLKSTKLPTQSGKVFKKRYQNENRVIEDLFLV +>460519_cbs-7435_MATkp_MATa|MATa2 +MTVTTFLSSSFDSVAFAMTCVSDKSYKTTAGKGLSGKKFNLSTHRPRNKFIISKSIFSKVIQRYYPDLSFEENSKIASYLWKHRFGCFDKYYEYLQIEESKFTSLVTSKIDQLAFKAVPTEKTTSQCTFRFSSSPKRRRTVNLNILKPLCKRVYSQQFHSPLTGITTQDIFVAEENNV +>KAM3161349.1|Mating_type_a2_protein +MILSELNKVENMNWTEGYAECHLPKVANQIKYKFGAQKTLGKDRFPCIAQRKCPKQICHFVTSGKTDKIKQSGLKSRNKFILMRGLLHCMVQQLRNCSDPFNANRAKVEEVSKIASAIWRTNKGIFQSYHEMLAVFEDSKSTNITISKPTLKKLTEAIGRECIPSTYSYETLTNGADTTAMESKKTYKAFCGRFSLKEATGRVRKKKPTQTFALRSAKIEDIFLI +>CAO02579.1|mating_type_a2_protein +MESEAWTQGYVECQVPRKCQNSREIKKGIQKHNTFPSIGREQAHLLNYQFISDHKKTKQNRSPSSKPKNKFILMRSSLHGVIQRLQFFSEAESLKESKVELVSKIASKLWRINKGAFQTYHELLAQFDEYKSFNKPTNKLSLKKLTEAIGRECIPSTYSYETLTNGAHTTAIESKKTYKAFCGRFSLNEATGRVRKKKPIQTFALRSANIEDVFLI +>4909_cbs-573_MATpk_MATa|MATa2 +MAKLTDSGSTRNRSESVSQNVGAVEFVQVVKQEHGYDEKEYKKILRSEIEKESKKPRNIFLIYRSLVKQYLMNYLSIKGFTEISEESGKLWKSRSSEVEHYVKYLAEQEERFFENEQTQRLKDRSSSASEKLKEKTLSQLTVDGYNGREPKGETVKKDVFRVYKPKKRKRSGVRSKMKSLRSKQNRVEDIFCVPNIEFQDIK +>4932_s288c_MATsc_HMRa|MATA2 +MRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>4952_w29_MATyl_A|mata2 +MENTILHIHSFQLPQTEQPYPEAMLFDRDTSDSRTVLTQKPNGLEISLNFLQYDGHKGLFIRQDSRTNEPEYIEPKVLKPRNSYILFRNATSRCSQSIDPNSAFVSRVSSYIWGSGIPNPIVRRWFKYMGFFEALYHEVDYPEYIPNKLPKSPKKPKVQKGATKSKKKGAKGKNKVTALQVLVGPTVGVGGRMTVSPMTAFFSNNSHVTCYDPNFVLDTPTREFLMMPLDDITLPSQGPRSDSQEQHVPRQPPDGQDYFDILDIDDFVSPYDDLTTRSFTNRQLFKHATLG +>BBB04550.1|mating_type_a2 +MDFASECVLPKVVVKTESHKDFLFFKNKKKPQSNSLQFKFVGQKVNETNRVAEINLSSEVNKGSSKETNQIITRPRNCFIIMRSIFHNVIVRSLQKYEISSLQHVSAITSQLWGKNDGIFQLYFELLSQFEEHWHLNIYPEYRYHKVNKISRQLENKLVYQNMLDRMRYFTASSLIANLEDILILPPAHPPPSSSTYTYPALRRPAAAAAAKAQAQAQAQAQAANAQAANPAAPAPPATNAERNAKTKPAPGGKVSKQRIHPKKKPSPPPRLHRPASAVQQIAFFETFHQQNFNTPRFSFTVYSC diff --git a/results/2026-10-30_mat_hmms/data/set_alpha1.faa b/results/2026-10-30_mat_hmms/data/set_alpha1.faa new file mode 100644 index 00000000..8b6d4113 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/set_alpha1.faa @@ -0,0 +1,24 @@ +>5476_sc5314_MTL_alpha|MTLalpha1 +MGNKKKTRKTVPKEFISLFRVHSGRDAPRRDTREVQKSKKHGFRFTSLPDLPVASNALQELLLEYGLLNDIKWDSKGLKASKNKKTKLKPINSFIAFRSFYSRTISNPEHQRELSSKLADVWTQESNQEVWKQYTQSYNNYLLLPDAKLNFVDWLCEALDYTIDNTTPQIEDISLTSYNQLLSGTIEDVYIMK +>42374_d1558_MTL_alpha|MTLalpha1 +MGNKKRSMKTVPKEFTSLFRVHPGRDPPRRDTREVQKSKKHGFRFTSLPDLPVASNALQELLLEYGLLNDIRWDSKGSKASKTKKIKLKPINSFIAFRSFYSRTISNPEHQRELSSKLAEVWTQESNQEVWKQYTQSYNNYLLLPEAKLNFVDWLCEALDYTIENATPSLEDISLTSYNQLLSGTIEDVYMLK +>498019_b11221_MTL_alpha|MTLalpha1 +MVSNEDIHLKSKEANFLMIRTGLSIPALPEPSETIINSLAECDASMDDIDLFKPSNRSKRRKAVKRRRLNGFIAFSSYYSRAAKSHSSQRKLSSLLAKVWKEEANKHVWSTYASVYNETGGNDDFILWLEKNLEQNEKDSTRSFTQKRKHHAFINDIEDVFLTSQANKNLAN +>4959_cbs767_MTL_A|MTLalpha1 +MTSEVAKVIPKRFTSRFTVSKTGKRSNSNIKNEAIDIGRQRKVKSMQVVFFTELPSLPTPSIDLQEILIRFNQATRNCAGDSSWGSQLFDMKVPTRKSRIRCKKTRCLNGFMAFRSFYSRSIYNVEHQRELSTLLGKLWKNEPNKAIWNRYAIEYNSQAINQDFVDWLFKALGLEVETFPIPYTSTVRNESWTFSSRNNENAVEDIYYIGTV +>28985_nrrl-y-1140_MATsc_HMLalpha|MATALPHA1 +MKSNAPTFKVAVSKRSCSSVRKTSKKIRSGMIRKPSVSSRYRKHEGVNLYMSKVTPTSIPAPPQVLVAYIKEKVKTLNKSEVLMSLGNSNQLSSRDVKTKTKKKQINDFIAFRSYYSRLLNGILTQTELSTIISKHWTVDKQTRKNWELIAQEYNCDASGKHFFNWLEVNYGIDKQWLYEIVQYEECLTPTTKKPYVENIYNSGFKQRIDSPNQKDITFSQSDNSADWLVDPIFFDTEFLATSFDRTNLIISDCYSLMNSV +>460519_cbs-7435_MATkp_MATalpha|MATalpha1 +MLPPQPKSFKGIQKPFKCSQYAHSTLDNGNMLQHTKHPELVTYQLETPRTRSLQQIESHLLQETTLGIKRTGENQYSEKRTKSSRSYMSKRINAFIAFRSYYSRQVNDYNKQISISRLLSVAWKNENKELWRSYALLYNSSNSNIPFVDWLQTKGPKNSKRTSTVTKSFISWNELLSTRNKIEDVFLDLQ +>381046_cbs-6340_MATsc_HMLalpha|MATALPHA1 +MNSTKPSFKVLIKKKGKTLNRRLKNSSKSYRENGANLYMSYSKPQAIPKPPNTLINLVRSKKTSQSSRKCLKNDYILKELDVKKLSIYSNNTVLKKKINPFIGFRSYYAKFAKGRVRQQELSKILSEYWTKNSKIHSVWEFFTQHYNREVTSMCFTLWLEENYQQSYEESELDSECMAKRSQPYIEDLYSGTGEFLTKLTSRELLDISGNHSGFQGSLNSLPFFEEHNRTTDWMLSSILQCDQVPYYPN +>4929_nrrl-y-2075_MTL_alpha|MTLalpha1 +MWYPTTSQNTLPLEIIRSMGINKHSVPSKYATSFSARQKRPSKSVRSTTKHVRKQEIVAIPLLPPLPQPSEHLKSVLLTINNMDHSNMSATASRPSFSGKGRKVQCAKINGFFAFRSFYAKSIKNIHYQRILSSLLSAAWKAEESKETWRRYAIIYNESRRDSDFVQWLCKSLNLYESGGDKVEINDGIIQTGNYNSMDIHVEDVYLVPK +>1005962_dl-1_MATog_MATa-MATalpha|MATalpha1 +MFLAFFVIARVGLQNLCLQRKFPPHGDSAKMALDGLNLSFLTHLPQIPTTSPLLKRLLSEYGAIDNGNDSSNKVAPKKSRRHRVQKQKKRLNGFMAFRSFYSRNIANYNSQKALSQDLADAWNREKHQQIWSLYAIQYNHSKSKEPFSIWLEMKLNKKLRKNNSDDPTTRREINIMSDIVVEDVFQECSS +>460523_cbs-4732_MATog_MATa-MATalpha|MATalpha1 +MMFHVLCFFGNRTSRVANLCLQLKFPPHNDSPKMALDELNLSFLTHLPQIPTTSPLLKRLLTEYGAIDNGKDSLNKVAPKKSSRHRVQKEKKRLNGFMAFRSFYSRNIANYNSQKALSQDLADAWNREKHQQIWSLYAIQYNHSKSKEPFSRWLEMKLSNKLGKFNSDDPTTRREINVMSHIVVEDVFQECSSQY +>4909_cbs-573_MATpk_MATalpha|MATalpha1 +MARMWNKSSTLRVKKQRPSRICKTTTKATIFIAKPTPRYVIPLEVEELLGKYISRNNLDLRTLSNVKKKNKKHKTVVNGFTAFRTYYSKFGKTYEDQENLSKELANLWKQTPSIQSTWRGYSEEYRASETDLPFVEWFDTYKSLIKPEPEKRDYITQTTTMSHLTVEDVYDNLGSNSMN +>4932_s288c_MATsc_MATalpha|MATALPHA1 +MFTSKPAFKIKNKASKSYRNTAVSKKLKEKRLAEHVRPSCFNIIRPLKKDIQIPVPSSRFLNKIQIHRIASGSQNTQFRQFNKTSIKSSKKYLNSFMAFRAYYSQFGSGVKQNVLSSLLAEEWHADKMQHGIWDYFAQQYNFINPGFGFVEWLTNNYAEVRGDGYWEDVFVHLAL diff --git a/results/2026-10-30_mat_hmms/data/set_alpha2.faa b/results/2026-10-30_mat_hmms/data/set_alpha2.faa new file mode 100644 index 00000000..82c36a6a --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/set_alpha2.faa @@ -0,0 +1,50 @@ +>5476_sc5314_MTL_alpha|MTLalpha2 +MNSHSEALFEINQRLSSHIRTMSNFPVFDPQKITNDVEANIKRMTSILKSKRLTREDEDLMKMINDCSKVLHEMLLERIMLQQDSIQFFTEKEASDSPFSNSADTIDEDDDKKIKSRRLTKKQLLVLEGWFQKHKNHPYSQKDQTNLLIKSTKLSKSQVQNWISNRRRKEKNTKVSPELAALLLTG +>42374_d1558_MTL_alpha|MTLalpha2 +MNTHSEALFQINQRLLFHIRTLSNFPVFDPQKITSDVETDIKKMVSIMKFKRLTNEDEAIMKMINDCSKVLQEMLLERIMLQQDSIQFFTEKEASDSSFSNTVDDAIEDGDDRKTKSRRLTKKQLLVLEGWFQKHKNHPYSQKDQTNLLIKSTKLSKSQVQNWISNRRRKEKNTKVSPELAALLLTG +>XP_003956854.1|homeodomain_mating_type_protein_alpha2_K +MNKIQIQDLLNSPRDVDTKQELRDINDKLFALCSKLPSSEALLEAEFHLELRDIMLNLSKLQDTSRMGSEEKHLAYTAHQLTCTMITLIKGMKNFHGTGGHSYSSSKSESDDKTVFNVVTQDMMNLNRTGISYRGHRFSKENVKVLERWYTAHIDRPYLNRQSTEYLISKTGLSRVQIKNWVSNRRRKEKSVHVSPELIQLLQKKS +>28985_nrrl-y-1140_MATsc_HMLalpha|MATALPHA2 +MSRIPIHSLLNPSESCKSISNVPSNYRDLSTFNKERAKVITTFQEMFYSMLENNDDYNKIESLIRNFQPKLTWSHKCESLTFKQKAYLTAIIQKSIKSLLVLLKEKGKMREIEFSRKEVRKINKYRQSSKNFESVNIKILTQDLMHSNNNEFKKGKRFPKSHIQLLENWYSMNRRNPYLAENDLAYISKNTTLTKTQIKNWLANRRRKDKITEVSSDIRNILN +>CCA89264.1|mating-type_alpha2_protein +MSKIPVQSLLNPESPREHFYKTYEIEHKQSQVDRKIQNLLTEFKRVFQLVLLSREVFIQPNYVEIDEFVKTIRQTLIYGSGEGELNTIISRNIFNSVLCLLIVLQEKQKISLLENNILSKVESFKERRSENTNTVEFRILSSEHLNPTLNKVGKKNRFPKKSIKLLENWYECNRNSPYLTSNHLDYLKCNTGLNKTQIKNWVANRRRKDKNSRVSLDIKNIFRQQ +>460519_cbs-7435_MATkp_MATalpha|MATalpha2 +MISQLLRVHKTLMRILQGISHNALHSNWTSICNQIGSSQRKLSSLLEQGIDQNDQTLREILVQINRMCRAIALMLENKRMLKNDVEIILQQILDSNSTERKRRLPREKVRILENWFADHFQHPYATQQDFDELVHLTSLSKTQIRNWISNKRRKERSSTVSTELLQALNKSPS +>381046_cbs-6340_MATsc_HMLalpha|MATALPHA2 +MSHISVSSLLNLPVFQASHQEKATSPITSQSCLVASGKQRKFNATAKKRLERWFCSNIQYPYPNKIQILSLSKNTNLSIKQIQNWMSNRRRKSRKESIDSLLEPILNS +>CAO02574.1|mating_type_alpha2_protein +MGRQLVSEMLFLADAIGKKILSFKFLNSAARGNQKVSKYPHNISMHLENWFHLNSSHPYLTRKQISLLSERTNLSSKQIRNWMSNRRRKSKKNPIDSSLHSLLNLSSIYS +>CAL1760704.1|mating-type_protein_Alpha2p +MTTMNKIPIQHLLNPTSNKRLYKSDLKQINTELMYICSSLPEGIFQNIKDVKIHLHDIVTKLGIIKGQGKLDNEERYLIKISFQLATIVANFLKSVEEEKKPSKDSKKKKKQGKSSKEEEVVFNVVTQNMMNMDNVKQNSFRGHRFSKQNVDVLEEWYSIHKHKPYLDKKSIERLQSQTQLSRTQIKNWVSNKRRKEKTVQVSSEILQLINGK +>SMN20089.1|MATALPHA2 +MNKIPIRDLLNPASKRRQYKPELKQINTKLIHICSSLPEGIFENIKEIKMDLHEIITKLSIIKREGRLDSEERYLIKISFQLATIVANFLNSNTQENIKPIKEQKKKKIEKKIPKEDEVVFNVVTQNMMNMDNVKQNSFRGHRFSKQNVEVLEEWYSIHKHKPYLDKKSTEVLQYQTQLSRTQIKNWVSNKRRKEKTVQVSSEILQLINGK +>XP_070910655.1|homeodomain_mating_type_protein_alpha2 +MSIKKITIPDLLNPSNQENLKEKLEEINKQLISLCSSLPKRNCVPAPSSDILRSLSRTKLDSQEIRLIKTTYRLSTLLSKLQEKDVVFNVVTKDHLLRNGAPNPYAQPYRGHRFTKENVHTLEAWYSNHIDKPYLDPKSLHSLAQKTNLSTVQIKNWVSNRRRKQTSPSPLTKI +>AAO25604.1|ALPHA2 +MESKKIAITELLNPSNQENLKEKLQEINKQLISLCSSLPKRNWVPAPSSDILRSLSRTKLDSQEIRLIKTTYRLSTLLSKLQEKDIVFNVVTKDHLLKNGTPYNPYPQPYRGHRFTKENVHTLEAWYSNHIDNPYLDPKSLQSLAQKTNLSKIQIKNWVSNRRRKQKHPPFLLI +>QHS64598.1|MTLalpha2 +MSKKSRISITHLLNPIQEENLKEKLQEINNQLISLCSSLPKRQSLPGPSSDILRFLSRNNLDPQEIGLIKTTYRLSTLLSKLREHEIVFNVVTKDHLLKKGVPNHYAASYRGHRFTRENVQILETWYRNHIDNPYLDHNSQQYLAQKTNLSKIQIKNWVANRRRKQKSIYISLFDIHNIESGEYAYIEKVCYIIIIICHLFLVFSLSCLT +>CAE84417.1|MATalpha2_transcription_factor +MDDMLTKIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKLCKSSMEGFTTERSLKYTRDCPQYAAVTTSQPRYRIEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTRCSPNDVV +>1005962_dl-1_MATog_MATa-MATalpha|MATalpha2 +MSSYEEQCSAPDLLLLLDRLIDSITESDLSLEDELYWDSHVHSLMAVITHTVSSPSGTPMDDMLGQIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKFCKSSMESFTTERTIEYTRDCPPYAAVTTSQPGYKTEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTDCSPNDVV +>460523_cbs-4732_MATog_MATa-MATalpha|MATalpha2 +MDDMLTKIQERTKCLIYILMERQIRAARLEQLLLDYISCKPHDKLCKSSMEGFTTERSLKYTRDCPQYAAVTTSQPRYRIEKRSKRFPKTAQMELENWYTENEDNPYLSKRDLQQLVHKTGLCAPQVRNWVSNRRRKERTLTISKELSDLLNQDTRCSPNDVV +>QFP92360.1|mating-type_protein_ALPHA2 +MKEIDSESFSGSINDSKRLDKPMSSSPEVDFSNPLETSAISETLTLLNSALDTIVDYISTDAADSGSDEHWGRLIYSFSKALQEHTQQSISKYDHGVGIQNVFLKTRCILYILIERKILKTKLEQLLLDHLSCLQFQRQFGSSVMDYEISAPASFIEKPHILGFSKSSNIHSNKRFSKSSQLILEQW +>4909_cbs-573_MATpk_MATalpha|MATalpha2 +MQHDELLEVYIHLKDVLRQLAETPIDEELSSTDLLKDMLNIKEILESGSPLQKQSCELIRMIRRISTSIYDMISQRSTLKTKYGLDLKSWDLDNSMMNTEDTNLTKNMDIHNSSSIDIDIDDEEISTALRDEGIHSSFCNTDSNSIKTKDGSSRNGKRLPKETIAVLNKWFNDNIENPYVQKEDISYLKAKTNLQASQIKNWVSNKRRKRKGSKVSPSIVEILN +>4932_s288c_MATsc_MATalpha|MATALPHA2 +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>XP_018223412.1|homeodomain_mating_type_protein_alpha2_D +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>XP_056080738.1|homeodomain_mating_type_protein_alpha2_S +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>QHS71949.1|Matalpha2 +MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE +>XP_003685884.1|homeodomain_mating_type_protein_alpha2_T +MNKIPINKLLNPSQHYNLTEKLQQINITLSNLCTKLPDTITDLTEADHRELQDILLYLTTVVKQQELKKEEIMLVKTTYQLCTTLTLMVKSCKQENDKENKENEIIKYESGTSDNSETNSSCNSSDNEDKNSILVFNVITQDMMNNNKKNNKSYRGHRLPKKNVQYLEDWYMDHRKNPYLNEINIKLLMSKTSLSRIQVKNWISNRRRKEKSITISPEVSALLRDNKTITS +>BAX02933.1|alpha2_transcriptional_factor +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKEDQKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRSNKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVSELVNGSKQGPEGSGT +>BBB04552.1|mating_type_alpha2 +MNKIPIEQLLNPVHQEFSHEKLKNINNKLLNLCSKLPAENEQFDEEKAELERILPSLNKLIREDGSSKEDQKMVHKMYQLSSILSVLLNEVSELRAKRRQFLRSKPMHHISYEAALGNKTKEKMVFNIVTQDTLHSVKRSNKTYRGHRLPKCVTYSLESWFNKNIKHPYLNKSSMKELMMETKLSGPQIKNWVSNRRRKEKSLTVSFEVSELVNGSKQGPEGSGT diff --git a/results/2026-10-30_mat_hmms/data/set_meta.tsv b/results/2026-10-30_mat_hmms/data/set_meta.tsv new file mode 100644 index 00000000..4092d0c0 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/set_meta.tsv @@ -0,0 +1,81 @@ +class species clade source id len +a1 Candida albicans Serinales curated 5476_sc5314_MTL_A|MTLA1 210 +a1 Candida anglica Serinales ncbi CAK7898777.1|MATa1_protein 161 +a1 Candida tropicalis Serinales ncbi XP_002546761.1|Mtla1p 148 +a1 Candidozyma auris Serinales curated 498019_b11205_MTL_A|MTLA1 166 +a1 Clavispora lusitaniae Serinales curated 36911_cl143_MTL_A|MTLA1 164 +a1 Debaryomyces hansenii Serinales curated 4959_cbs767_MTL_A|MTLA1 173 +a1 Hyphopichia burtonii Pichiaceae ncbi XP_020074339.1|Mtla1p 208 +a1 Kluyveromyces lactis Saccharomycetaceae curated 28985_nrrl-y-1140_MATsc_MATa|MATA1 228 +a1 Kluyveromyces marxianus Saccharomycetaceae ncbi XP_022675170.1|mating-type_protein_A1 213 +a1 Komagataella phaffii Pichiaceae curated 460519_cbs-7435_MATkp_MATa|MATa1 225 +a1 Lachancea thermotolerans Saccharomycetaceae ncbi KAM3161348.1|Mating_type_a1_protein 142 +a1 Lachancea waltii Saccharomycetaceae ncbi CAO02580.1|mating_type_a1_protein 117 +a1 Lodderomyces orthopsilosis Serinales ncbi XP_003870289.1|Mtla1p 183 +a1 Lodderomyces theae Serinales ncbi XP_051608499.1|Mtla1p 196 +a1 Monosporozyma servazzii Saccharomycetaceae ncbi CAL9736377.1|mating-type_protein_A1 88 +a1 Monosporozyma unispora Saccharomycetaceae ncbi CAL9731627.1|mating-type_protein_A1 88 +a1 Nakaseomyces delphensis Saccharomycetaceae ncbi CAE84400.1|MATa1_protein 122 +a1 Ogataea angusta Pichiaceae ncbi CAE84419.1|MATa1_transcription_factor 181 +a1 Ogataea parapolymorpha Pichiaceae curated 1005962_dl-1_MATog_MATa-MATalpha|MATa1 187 +a1 Ogataea polymorpha Pichiaceae curated 460523_cbs-4732_MATog_MATa-MATalpha|MATa1 181 +a1 Pichia kudriavzevii Pichiaceae curated 4909_cbs-573_MATpk_MATa|MATa1 269 +a1 Priceomyces carsonii Serinales ncbi XP_062812646.1|Mtla1p 188 +a1 Saccharomyces cerevisiae Saccharomycetaceae curated 4932_s288c_MATsc_HMRa|MATA1 126 +a1 Scheffersomyces amazonensis Serinales ncbi XP_064741610.1|Mtla1p 190 +a1 Scheffersomyces coipomensis Serinales ncbi XP_064762305.1|Mtla1p 130 +a1 Scheffersomyces stipitis Serinales ncbi XP_001386328.2|Mtla1p 136 +a1 Yarrowia lipolytica Dipodascales curated 4952_w29_MATyl_A|mata1 176 +a1 Zygosaccharomyces rouxii Saccharomycetaceae ncbi BAX02937.1|a1_transcriptional_factor 144 +a1 Zygosaccharomyces sp. Saccharomycetaceae ncbi BBB04551.1|mating_type_a1 144 +a2 Candida albicans Serinales curated 5476_sc5314_MTL_A|MTLA2 201 +a2 Candida anglica Serinales ncbi CAK7898773.1|MATa2_protein 236 +a2 Candidozyma auris Serinales curated 498019_b11243_MTL_A|MTLA2 152 +a2 Clavispora lusitaniae Serinales curated 36911_cl143_MTL_A|MTLA2 176 +a2 Debaryomyces hansenii Serinales curated 4959_cbs767_MTL_A|MTLA2 251 +a2 Kluyveromyces lactis Saccharomycetaceae curated 28985_nrrl-y-1140_MATsc_MATa|MATA2 257 +a2 Kluyveromyces marxianus Saccharomycetaceae ncbi XP_022673680.1|mating-type_protein_A2 254 +a2 Komagataella phaffii Pichiaceae curated 460519_cbs-7435_MATkp_MATa|MATa2 178 +a2 Lachancea thermotolerans Saccharomycetaceae ncbi KAM3161349.1|Mating_type_a2_protein 225 +a2 Lachancea waltii Saccharomycetaceae ncbi CAO02579.1|mating_type_a2_protein 216 +a2 Pichia kudriavzevii Pichiaceae curated 4909_cbs-573_MATpk_MATa|MATa2 202 +a2 Saccharomyces cerevisiae Saccharomycetaceae curated 4932_s288c_MATsc_HMRa|MATA2 119 +a2 Yarrowia lipolytica Dipodascales curated 4952_w29_MATyl_A|mata2 291 +a2 Zygosaccharomyces sp. Saccharomycetaceae ncbi BBB04550.1|mating_type_a2 305 +alpha1 Candida albicans Serinales curated 5476_sc5314_MTL_alpha|MTLalpha1 193 +alpha1 Candida dubliniensis Serinales curated 42374_d1558_MTL_alpha|MTLalpha1 193 +alpha1 Candidozyma auris Serinales curated 498019_b11221_MTL_alpha|MTLalpha1 172 +alpha1 Debaryomyces hansenii Serinales curated 4959_cbs767_MTL_A|MTLalpha1 212 +alpha1 Kluyveromyces lactis Saccharomycetaceae curated 28985_nrrl-y-1140_MATsc_HMLalpha|MATALPHA1 261 +alpha1 Komagataella phaffii Pichiaceae curated 460519_cbs-7435_MATkp_MATalpha|MATalpha1 190 +alpha1 Lachancea thermotolerans Saccharomycetaceae curated 381046_cbs-6340_MATsc_HMLalpha|MATALPHA1 249 +alpha1 Meyerozyma guilliermondii Serinales curated 4929_nrrl-y-2075_MTL_alpha|MTLalpha1 210 +alpha1 Ogataea parapolymorpha Pichiaceae curated 1005962_dl-1_MATog_MATa-MATalpha|MATalpha1 190 +alpha1 Ogataea polymorpha Pichiaceae curated 460523_cbs-4732_MATog_MATa-MATalpha|MATalpha1 195 +alpha1 Pichia kudriavzevii Pichiaceae curated 4909_cbs-573_MATpk_MATalpha|MATalpha1 179 +alpha1 Saccharomyces cerevisiae Saccharomycetaceae curated 4932_s288c_MATsc_MATalpha|MATALPHA1 175 +alpha2 Candida albicans Serinales curated 5476_sc5314_MTL_alpha|MTLalpha2 186 +alpha2 Candida dubliniensis Serinales curated 42374_d1558_MTL_alpha|MTLalpha2 187 +alpha2 Kazachstania africana Saccharomycetaceae ncbi XP_003956854.1|homeodomain_mating_type_protein_alpha2_K 206 +alpha2 Kluyveromyces lactis Saccharomycetaceae curated 28985_nrrl-y-1140_MATsc_HMLalpha|MATALPHA2 223 +alpha2 Kluyveromyces marxianus Saccharomycetaceae ncbi CCA89264.1|mating-type_alpha2_protein 225 +alpha2 Komagataella phaffii Pichiaceae curated 460519_cbs-7435_MATkp_MATalpha|MATalpha2 173 +alpha2 Lachancea thermotolerans Saccharomycetaceae curated 381046_cbs-6340_MATsc_HMLalpha|MATALPHA2 108 +alpha2 Lachancea waltii Saccharomycetaceae ncbi CAO02574.1|mating_type_alpha2_protein 110 +alpha2 Maudiozyma bulderi Saccharomycetaceae ncbi CAL1760704.1|mating-type_protein_Alpha2p 213 +alpha2 Maudiozyma saulgeensis Saccharomycetaceae ncbi SMN20089.1|MATALPHA2 211 +alpha2 Nakaseomyces bracarensis Saccharomycetaceae ncbi XP_070910655.1|homeodomain_mating_type_protein_alpha2 174 +alpha2 Nakaseomyces delphensis Saccharomycetaceae ncbi AAO25604.1|ALPHA2 174 +alpha2 Nakaseomyces glabratus Saccharomycetaceae ncbi QHS64598.1|MTLalpha2 210 +alpha2 Ogataea angusta Pichiaceae ncbi CAE84417.1|MATalpha2_transcription_factor 163 +alpha2 Ogataea parapolymorpha Pichiaceae curated 1005962_dl-1_MATog_MATa-MATalpha|MATalpha2 222 +alpha2 Ogataea polymorpha Pichiaceae curated 460523_cbs-4732_MATog_MATa-MATalpha|MATalpha2 163 +alpha2 Ogataea thermomethanolica Pichiaceae ncbi QFP92360.1|mating-type_protein_ALPHA2 187 +alpha2 Pichia kudriavzevii Pichiaceae curated 4909_cbs-573_MATpk_MATalpha|MATalpha2 224 +alpha2 Saccharomyces cerevisiae Saccharomycetaceae curated 4932_s288c_MATsc_MATalpha|MATALPHA2 210 +alpha2 Saccharomyces eubayanus Saccharomycetaceae ncbi XP_018223412.1|homeodomain_mating_type_protein_alpha2_D 210 +alpha2 Saccharomyces mikatae Saccharomycetaceae ncbi XP_056080738.1|homeodomain_mating_type_protein_alpha2_S 210 +alpha2 Saccharomyces paradoxus Saccharomycetaceae ncbi QHS71949.1|Matalpha2 210 +alpha2 Tetrapisispora phaffii Saccharomycetaceae ncbi XP_003685884.1|homeodomain_mating_type_protein_alpha2_T 231 +alpha2 Zygosaccharomyces rouxii Saccharomycetaceae ncbi BAX02933.1|alpha2_transcriptional_factor 225 +alpha2 Zygosaccharomyces sp. Saccharomycetaceae ncbi BBB04552.1|mating_type_alpha2 225 diff --git a/results/2026-10-30_mat_hmms/data/ss_genome_scan.tsv b/results/2026-10-30_mat_hmms/data/ss_genome_scan.tsv new file mode 100644 index 00000000..61e1807d --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/ss_genome_scan.tsv @@ -0,0 +1,30 @@ +segment a2 a2_no_Pichiaceae a2_no_Saccharomycetaceae sacch_a2 a1 alpha2 alpha1 +CM143129.1:527654-528454(-) 77.6 81.4 64.7 65.9 +CM143129.1:537367-538167(+) 77.6 81.4 64.7 65.9 +CM143129.1:502064-502864(-) 77.1 80.9 64.2 65.8 +CM143131.1:299861-300331(+) 61.3 21.4 +CM143129.1:503643-504203(+) 60.9 19.8 +CM143129.1:529186-529746(+) 60.7 20.7 +CM143129.1:536074-536634(-) 60.7 20.7 +CM143129.1:471889-472461(+) 60.6 20.7 +CM143127.1:500713-501303(-) 60.4 20.6 +CM143128.1:2743403-2743993(+) 60.4 20.6 +CM143130.1:2217565-2218167(-) 60.4 20.5 +CM143126.1:1289709-1290320(+) 59.3 20.3 +CM143127.1:1242710-1244869(-) 38.5 +CM143130.1:1755952-1756965(-) 36.3 +CM143129.1:1323823-1324638(-) 35.2 49.5 +CM143127.1:3226939-3228111(+) 27.4 38.1 +CM143129.1:470238-470948(+) 75.6 +CM143126.1:1288075-1288779(+) 75.0 +CM143130.1:2219091-2219720(-) 74.5 +CM143131.1:298037-298915(+) 72.3 +CM143127.1:502232-503110(-) 71.4 +CM143128.1:2741596-2742474(+) 71.4 +CM143131.1:298990-299715(-) 115.5 +CM143130.1:2218372-2219016(+) 115.5 +CM143129.1:471023-471748(-) 115.5 +CM143126.1:1288850-1289575(-) 115.3 +CM143127.1:501436-502161(+) 115.3 +CM143128.1:2742545-2743270(-) 115.3 +CM143127.1:528749-529090(+) 15.5 diff --git a/results/2026-10-30_mat_hmms/data/test_proteins.faa b/results/2026-10-30_mat_hmms/data/test_proteins.faa new file mode 100644 index 00000000..01c857a3 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/test_proteins.faa @@ -0,0 +1,46 @@ +>Pkud_MATa2_control +MAKLTDSGSTRNRSESVSQNVGAVEFVQVVKQEHGYDEKEYKKILRSEIEKESKKPRNIFLIYRSLVKQYLMNYLSIKGFTEISEESGKLWKSRSSEVEHYVKYLAEQEERFFENEQTQRLKDRSSSASEKLKEKTLSQLTVDGYNGREPKGETVKKDVFRVYKPKKRKRSGVRSKMKSLRSKQNRVEDIFCVPNIEFQDIK +>PF_JOY008_MATa2 +MVTIGAQCCRNGEAPPSISTFPILSKISFTLTKTDLPTKVFWEKKQFPVASRLAAGANAPRSPKEITFAHLSSGTSTKSIYVYAPHNNVARPRNKFIIARTILSRIVKTRHPWSTNDVSKIISKIWGSGNRLFLSYFDYLSFLENESHECRYPLYHYNPRSRSTVAKASACTASAKNSELEQNMSVIFLNDPENSIKQSKQKTEKASASDIKDMSSYVKLCTSVYEEDDCKRRGVDALHDSDDYKDHVLVLTLNEPNMKAPVDSASTNARTTYKVTKPTKKKKTPPHLELVFSKFRSPLYGEAEDICINSSPM +>PF_JOY008_MATa1 +MATRDQIAYKEASEKLDALIEFSSNFSTNPTKPRYDKIMLEDPFEKDSLLSKVPAYARAALETLISNFELSLGRVMVGQSLSHKSNQSVIKTNKPLGPRLKDPEAYGHRIASYEVQSTTIEVTGSTSSPVFPGGGRKRNALPAKKRTSITPESKEFLERVFKAKRTPNSTERKVIAEKCGLTPLQVRVWFTNKRMRNKEG +>PF_TBRC4498_MATalpha1 +MIEVPLGALLALSDPYTFSSQGKMTGISKIVKKRNKQGVEVLRFRGCPASKVRGTRGAQGLHQCMERNNSMTSGEASVTIFAATKLPDIPSIPYTLQKQLDELRSQTTTSDRRKIKRLPQLQVSVNRDDTVIKRATKTKLNGFMAFRAFHSREISTIEHQRGISMSLATVWASDPPSQLVWQRFAAEYNQTVRYEPDLSFVDWLLSMSKGVDSKIDEPTKRTLSRAARVENIYVEKDGNLRHNTDPYALELQNKDVNNRYHNYLKERLQSAIDRPIVTLASDPSLSRSYIDPLVYEISSGTYEKS +>BC_MATa2 +MESLKIAHFKASAGNDSDLRIKKSILPFVSIPKGPSSGQKFTLTFEDSPSTSSKQYSNVARPRNQFIVARMILNKIVLANCNDTIANDDVSKIISIIWKTSSSPFQKYFKYLSLLEESWHEEHYPLYHYQPKVRSKTIKSLAPVLEQSRTMTGRISKCAPLSSLKKKSGFVNTSPHMMGTYVKLSTSSFRHSTVVSNVSINLVSKFRLTKGHTSGTTYKKRGPRSSKDDAYIKNNLWCLSGFDKTNYFEPLFYEDKIQNITLEDFKDYI +>BC_MATa1 +MNQKLITGQYQRLLFNTLADKYGSEVLELIDYSVEKPYFLSKSYRVKSSTISVNERSATESNCSDISPSSHSPPEASSMNTSNVCSVSEAVMASPDSNNNIQQKKRRRHISKEARSLLEKAFETTKNPTRKERAIIAEKSGMTALQVRVWFTNKRMRTKTRI +>BC_MATalpha1 +MPRKEVKAKWTSNPKKPYSAGFKLFSLPGLKHRNNALESKNPSKCTSPSNFQCLTFAATSLPKIPKLSADYERFIEGLKDHKAGQDSSTYIRQHLRRKRGPSRKRTKLNGFMAYRSFFSRNVATDQQGVLSSQLAKEWERNPEIQLVWKRYSTEYNLSVLSDRLPFFDWLLLVTSEPTCISENIYDNKDPGTFSRIQDVFNTNEADENHQFLKMSFDVSVIDPRIGQAFEHSI +>SS.1982.2.p3 +MSVLKISEIVSPNPKEDQLFNKKINKIMESLSNVYNSLLSELTVDSIVNNKMEVNAIKQKVVKILKEYSKISSQNRTSLLSVADFCDILIQLIGERYNLLNDIFYCQNRITRTLSNVPVVKNDEPAEEVSFNIVTQSQIKRSDYKALRKCRHFNTETRRKLEQWYKLSDFSKEAIEKFLNEVNLDVVQIKNWVSNKKRKEKKAISKGIIDVLKTFSPSNVYVMTGDL +>SS.1982.1.p3 +MESLSNVYNSLLSELTVDSIVNNKMEVNAIKQKVVKILKEYSKISSQNRTSLLSVADFCDILIQLIGERYNLLNDIFYCQNRITRTLSNVPVVKNDEPAEEVSFNIVTQSQIKRSDYKALRKCRHFNTETRRKLEQWYKLSDFSKEAIEKFLNEVNLDVVQIKNWVSNKKRKEKKAISKGIIDVLKTFSPSNVYVMTGDL +>SS.1982.2.p4 +MGILQQNYKTKCHKFTNYSFRKVKSATPIRYPTTFQNTFYFDSLPKIPKCYLKLEDLLTEFKKTPRMNEISDQCTEKKKNKAQKKPTKRKLNGFIAYRSYYSKNIHDTALQIEVSKRLATVWKDDPSADIWKRFAFEYNKSDENMPFLEWLDVKTSQMDKVNKQATNGCDFFLDFEVLNFICDIEGKYVVS +>SS.1982.2.p5 +MLIVDIFLSQSTLKSSKYTDKLRKYLEDRGVDIVVLMHDQLQTSNNSRYSRKTTEYLELLFDTNPRPNSRERLFIAKQCGLSAKQVRIWFTNRRARVKKTQKP +>SS.2002.2.p2 +MSDNEKIAITVGEMVAKLRDTEQCIIRTTGRTSADISISDFTKDTNSYVQYYHLTESEHIPEELKLSYKPLIERYQTYLNTIIKLSDAAIKSGFKVCDKDSVYNKTERMLIVDIFLSQSTLKSSKYTDKLRKYLEDRGVDIVVLMHDQLQTSNNSRYSRKTTEYLELLFDTNPRPNSRERLFIAKQCGLSAKQVRIWFTNRRARVKKTQKP +>SS.2001.1.p4 +MLIVDIFLSQSTLKSSKYTDKLRKYLEDRGVDIVVLMHDQLQTSNNSRYSRKTTEYLELLFDTNPRPNSRERLFIAKQCGLSAKQVRIWVSILLLLLLLLLLFFFFFFLLLLFYTNIYIYIYIYILFVWDGGGGGSSNLKFIFLFGKQYLELTSIFIVYQQTC +>SS.2002.1.p2 +MSDNEKIAITVGEMVAKLRDTEQCIIRTTGRTSADISISDFTKDTNSYVQYYHLTESEHIPEELKLSYKPLIERYQTYLNTIIKLSDAAIKSGFKVCDKDSVYNKTERMLIVDIFLSQSTLKSSKYTDKLRKYLEDRGVDIVVLMHDQLQTSNNSRYSRKTTEYLELLFDTNPRPNSRERLFIAKQCGLSAKQVRIWVSILLLLLLLLLLFFFFFFLLLLFYTNIYIYIYIYILFVWDGGGGGSSNLKFIFLFGKQYLELTSIFIVYQQTC +>BCreg_+0_0 +IDVGTLSSNILASHMVSFTSMSSDQTNTLRSWIYFRKDIQILQQRISHSFKKFKKTY +>BCreg_+0_182 +HELTSILRISNKRYVCTSKFVMNRYSTNNIDRRKEREDKIGDSISCLVSGLVKNDCP +>BCreg_+1_229 +CRDLLKTIVPKPITPICEHFSLHKLLGNSEIVNIASELLKPSGWQLERYKL +>BCreg_+2_26 +HTKELDLLQKRHSNTTAEDQSQFQEIQKDLLKSQLLLSEKAILVEALERFSTSTESPNNPDPFRFNIITQKMIREDGSIISRKKRLARQKVAILEDWYVFSHSTLFKFTINIV +>BCreg_+2_268 +NLQDGSLKDTSFENHYLIYFICFLPTVVFCCYKFCPFHERIQLNQSFFSYHFHDIPLHDRTW +>BCreg_-0_79 +NRSNSDSQSLYLSSCHPEGFKSSDAMLTISEFPNNLCKEKCSHIGVIGLGTIVFNKSRH +>BCreg_-1_0 +QAKLQCCKFRESGVTPLKFLQEAEMKTQSLYVKRHSPRPIMEWNIMKVITEKGLVELNSFMKGTKFVTTENYSREKANEIDQIVILKACIFQAAILKVSKVLMQC +>BCreg_-1_128 +GQSFLTSPDTRHEIESPILSSRSFLLSMLLVLYRFITNFEVHTYLLLEILSIEVSSCYDL +>BCreg_-2_227 +TMLIVNLNRVLCEKTYQSSRMATFCLANLFFLEIILPSSLIIFCVIILNLKGSGLLGDSVEVENRSRASTKMAFSESKS diff --git a/results/2026-10-30_mat_hmms/data/test_rank.tsv b/results/2026-10-30_mat_hmms/data/test_rank.tsv new file mode 100644 index 00000000..b96e5633 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/test_rank.tsv @@ -0,0 +1,24 @@ +protein a1:bits(bg proteins >=) alpha2:bits(bg proteins >=) a2:bits(bg proteins >=) alpha1:bits(bg proteins >=) a2_no_Pichiaceae:bits(bg proteins >=) a2_no_Saccharomycetaceae:bits(bg proteins >=) sacch_a2(existing):bits(bg proteins >=) +Pkud_MATa2_control -2(1554) 3(102) 167(0) 6(8) 43(0) 167(0) 52(0) +PF_JOY008_MATa2 0(540) 0(525) 73(0) 5(8) 78(0) 76(0) 77(0) +PF_JOY008_MATa1 111(0) 9(5) -1(816) -1(838) -1(874) 0(464) 0(457) +PF_TBRC4498_MATalpha1 2(210) 2(190) -0(526) 103(0) 2(142) 4(28) 1(155) +BC_MATa2 -1(941) 0(547) 81(0) 0(328) 81(0) 76(0) 82(0) +BC_MATa1 104(0) 23(3) 0(414) 1(154) 2(113) 1(285) 0(345) +BC_MATalpha1 -1(1114) -1(1197) 7(2) 86(0) 2(139) 7(2) 1(272) +SS.1982.2.p3 9(7) 81(1) -2(1339) -2(1482) -4(2045) -0(674) -0(630) +SS.1982.1.p3 12(3) 73(1) -3(1850) -1(1048) -2(1630) -1(705) 1(310) +SS.1982.2.p4 3(101) 2(214) 14(1) 118(0) 10(0) 17(1) 12(1) +SS.1982.2.p5 86(0) 29(3) 0(355) 1(306) 0(461) 1(263) 0(467) +SS.2002.2.p2 85(0) 27(3) -0(648) 0(449) -0(609) -0(671) 1(225) +SS.2001.1.p4 60(0) 20(3) -2(1233) -1(1090) -1(1238) -1(818) -1(1187) +SS.2002.1.p2 62(0) 21(3) -1(897) -1(847) -1(1035) -1(945) 0(339) +BCreg_+0_0 1(323) 2(205) -0(638) 0(449) 0(475) 0(421) 1(259) +BCreg_+0_182 1(376) 5(37) 1(210) -1(720) 1(246) 1(182) 2(129) +BCreg_+1_229 -2(1482) -2(1387) -2(1565) -1(730) -2(1607) -2(1508) -1(1177) +BCreg_+2_26 4(73) 37(3) 2(112) -0(664) -1(761) -0(671) 1(175) +BCreg_+2_268 -3(1790) 0(636) 0(469) -3(1947) 0(498) 0(466) 0(480) +BCreg_-0_79 1(491) -2(1707) -3(1779) -3(1874) -2(1739) -1(845) -2(1447) +BCreg_-1_0 -1(897) 0(530) 0(456) 0(402) -0(576) -0(479) -1(827) +BCreg_-1_128 -2(1394) -2(1613) -2(1227) -3(1806) -2(1598) -3(1823) -1(1251) +BCreg_-2_227 -2(1380) -2(1402) -2(1611) -2(1579) -2(1655) -2(1683) -2(1581) diff --git a/results/2026-10-30_mat_hmms/data/test_scores.tsv b/results/2026-10-30_mat_hmms/data/test_scores.tsv new file mode 100644 index 00000000..7f299658 --- /dev/null +++ b/results/2026-10-30_mat_hmms/data/test_scores.tsv @@ -0,0 +1,26 @@ +protein len a1 alpha2 a2 alpha1 a2_no_Pichiaceae a2_no_Saccharomycetaceae sacch_a2(existing) +Pkud_MATa2_control 202 -2.1 3.2 167.4 5.7 43.5 166.7 52.4 +PF_JOY008_MATa2 313 0.4 0.4 73.3 5.2 78.4 75.6 76.8 +PF_JOY008_MATa1 200 110.7 9.4 -0.8 -0.8 -0.9 0.0 0.1 +PF_TBRC4498_MATalpha1 305 2.1 2.2 -0.2 102.9 1.6 4.5 1.4 +BC_MATa2 269 -0.7 0.3 80.7 0.5 81.1 76.0 81.7 +BC_MATa1 162 104.1 23.0 0.1 1.4 2.0 0.6 0.4 +BC_MATalpha1 233 -1.0 -1.3 7.4 85.9 1.7 7.4 0.7 +SS.1982.2.p3 227 9.0 80.6 -1.7 -1.8 -4.4 -0.5 -0.4 +SS.1982.1.p3 200 11.6 72.6 -2.8 -1.1 -2.2 -0.5 0.5 +SS.1982.2.p4 191 3.3 1.9 14.2 118.3 9.8 17.3 12.5 +SS.1982.2.p5 103 85.6 28.8 0.3 0.6 0.1 0.7 0.0 +SS.2002.2.p2 211 85.1 26.7 -0.4 0.0 -0.3 -0.5 1.0 +SS.2001.1.p4 163 59.8 20.4 -1.5 -1.2 -1.5 -0.7 -1.2 +SS.2002.1.p2 271 62.4 20.7 -0.9 -0.8 -1.2 -0.9 0.4 +BCreg_+0_0 57 1.4 2.0 -0.4 0.0 0.1 0.2 0.7 +BCreg_+0_182 57 1.1 4.8 1.1 -0.6 0.9 1.3 1.7 +BCreg_+1_229 51 -1.9 -1.7 -2.1 -0.6 -2.1 -1.8 -1.2 +BCreg_+2_26 113 3.8 37.4 2.0 -0.5 -0.7 -0.5 1.2 +BCreg_+2_268 62 -3.0 0.0 0.0 -3.1 0.0 0.0 0.0 +BCreg_-0_79 59 0.6 -2.5 -2.6 -2.7 -2.5 -0.8 -1.7 +BCreg_-1_0 105 -0.5 0.4 0.0 0.2 -0.2 -0.0 -0.7 +BCreg_-1_128 60 -1.6 -2.2 -1.5 -2.5 -2.1 -2.6 -1.3 +BCreg_-2_227 79 -1.6 -1.7 -2.2 -2.0 -2.3 -2.2 -2.0 +BACKGROUND_max 36.0 80.6 19.4 8.5 9.7 23.6 16.6 +BACKGROUND_p99 5.3 5.2 3.5 3.8 4.0 4.6 3.6 diff --git a/results/2026-10-30_mat_hmms/fetch_public.py b/results/2026-10-30_mat_hmms/fetch_public.py new file mode 100644 index 00000000..bfc43e01 --- /dev/null +++ b/results/2026-10-30_mat_hmms/fetch_public.py @@ -0,0 +1,27 @@ +"""Public a1 / alpha2 / a2 proteins of Saccharomycotina (taxid 147537) from NCBI Protein by product name (E-utilities).""" +import json, re, sys, time, urllib.parse, urllib.request +def get(u): + for i in range(4): + try: return urllib.request.urlopen(u, timeout=60).read().decode() + except Exception as e: time.sleep(2 + 2 * i) + raise SystemExit("fail " + u) +E_ = "https://eutils.ncbi.nlm.nih.gov/entrez/eutils/" +Q = { + "a1": ['"mating-type protein A1"', '"mating type protein a1"', '"mating-type a1"', '"MATa1"', '"MAT a1"', '"mating type protein A1"'], + "alpha2": ['"mating-type protein alpha2"', '"mating type protein alpha2"', '"MATalpha2"', '"MAT alpha2"', '"mating-type alpha2"', '"mating type protein ALPHA2"'], + "a2": ['"mating-type protein A2"', '"mating type protein a2"', '"MATa2"', '"MAT a2"', '"mating-type a2"', '"mating type protein A2"'], +} +res = {} +for c, qs in Q.items(): + ids = [] + for q in qs: + term = f'({q}[Title]) AND txid147537[Organism:exp]' + j = json.loads(get(E_ + "esearch.fcgi?db=protein&retmax=300&retmode=json&term=" + urllib.parse.quote(term))) + ids += j["esearchresult"]["idlist"]; time.sleep(0.4) + res[c] = sorted(set(ids)); print(c, len(res[c])) +json.dump(res, open("data/public_ids.json", "w")) +for c, ids in res.items(): + with open(f"data/public_{c}.faa", "w") as fh: + for i in range(0, len(ids), 100): + fh.write(get(E_ + "efetch.fcgi?db=protein&rettype=fasta&retmode=text&id=" + ",".join(ids[i:i + 100]))); time.sleep(0.4) + print(c, open(f"data/public_{c}.faa").read().count(">")) diff --git a/results/2026-10-30_mat_hmms/fetch_public2.py b/results/2026-10-30_mat_hmms/fetch_public2.py new file mode 100644 index 00000000..84c337c4 --- /dev/null +++ b/results/2026-10-30_mat_hmms/fetch_public2.py @@ -0,0 +1,21 @@ +"""Second pass: NCBI Protein by gene symbol (MATA1, MATALPHA2, MTLA1 ...) in Saccharomycotina; merges into data/public_*.faa.""" +import json, time, urllib.parse, urllib.request +E_ = "https://eutils.ncbi.nlm.nih.gov/entrez/eutils/" +def get(u): + for i in range(4): + try: return urllib.request.urlopen(u, timeout=60).read().decode() + except Exception: time.sleep(2 + 2 * i) + raise SystemExit("fail") +G = {"a1": ["MATA1", "MATa1", "MTLA1", "mata1", "MAT-A1", "a1"], "alpha2": ["MATALPHA2", "MATalpha2", "MTLalpha2", "MAT-ALPHA2", "alpha2", "MATa2alpha"], "a2": ["MATA2", "MATa2", "MTLA2", "mata2", "MAT-A2"]} +old = json.load(open("data/public_ids.json")) +for c, gs in G.items(): + ids = set(old[c]) + for g in gs: + term = f'{g}[Gene Name] AND txid147537[Organism:exp]' + j = json.loads(get(E_ + "esearch.fcgi?db=protein&retmax=300&retmode=json&term=" + urllib.parse.quote(term))) + ids |= set(j["esearchresult"]["idlist"]); time.sleep(0.4) + ids = sorted(ids); print(c, len(old[c]), "->", len(ids)); old[c] = ids + with open(f"data/public_{c}.faa", "w") as fh: + for i in range(0, len(ids), 100): + fh.write(get(E_ + "efetch.fcgi?db=protein&rettype=fasta&retmode=text&id=" + ",".join(ids[i:i + 100]))); time.sleep(0.4) +json.dump(old, open("data/public_ids.json", "w")) diff --git a/results/2026-10-30_mat_hmms/hmm/a1.hmm b/results/2026-10-30_mat_hmms/hmm/a1.hmm new file mode 100644 index 00000000..51ea5852 --- /dev/null +++ b/results/2026-10-30_mat_hmms/hmm/a1.hmm @@ -0,0 +1,554 @@ +HMMER3/f [3.4 | Aug 2023] +NAME a1 +LENG 177 +ALPH amino +RF no +MM no +CONS yes +CS no +MAP yes +DATE Fri Oct 30 06:47:58 2026 +COM [1] loco.py +NSEQ 29 +EFFN 4.718872 +CKSUM 4180212094 +STATS LOCAL MSV -10.6005 0.70693 +STATS LOCAL VITERBI -11.1009 0.70693 +STATS LOCAL FORWARD -4.1764 0.70693 +HMM A C D E F G H I K L M N P Q R S T V W Y + m->m m->i m->d i->m i->i d->m d->d + COMPO 2.71331 4.29177 2.99347 2.49185 3.37261 3.17250 3.73191 2.98197 2.41415 2.55802 3.70807 2.88500 3.40536 2.94572 2.68292 2.48334 2.74652 2.93539 4.59151 3.72852 + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.00785 5.24683 5.96918 0.61958 0.77255 0.00000 * + 1 4.80034 6.16837 5.95605 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2.98518 4.58477 3.61503 + 0.01585 4.54859 5.27093 0.61958 0.77255 0.82146 0.57944 + 177 2.67749 4.98497 3.01227 2.45637 4.26610 2.92774 3.66962 3.70069 2.17604 2.63526 4.05057 2.64618 3.08079 2.79730 2.41361 2.68222 2.55949 3.34226 5.46100 4.10577 339 k - - - + 2.68701 4.42103 2.77563 2.73092 3.46279 2.40569 3.72578 3.29259 2.67746 2.69364 4.24600 2.90430 2.73540 3.18229 2.89884 2.37754 2.77539 2.98602 4.58560 3.61519 + 0.63759 0.75198 * 2.01061 0.14376 0.00000 * +// diff --git a/results/2026-10-30_mat_hmms/hmm/a2.hmm b/results/2026-10-30_mat_hmms/hmm/a2.hmm new file mode 100644 index 00000000..a0d14dcc --- /dev/null +++ b/results/2026-10-30_mat_hmms/hmm/a2.hmm @@ -0,0 +1,668 @@ +HMMER3/f [3.4 | Aug 2023] +NAME a2 +LENG 215 +ALPH amino +RF no +MM no +CONS yes +CS no +MAP yes +DATE Fri Oct 30 06:49:13 2026 +COM [1] loco.py +NSEQ 14 +EFFN 3.482910 +CKSUM 1770565654 +STATS LOCAL MSV -10.8843 0.70451 +STATS LOCAL VITERBI -11.8329 0.70451 +STATS LOCAL FORWARD -4.6728 0.70451 +HMM A C D E F G H I K L M N P Q R S T V W Y + m->m m->i m->d i->m i->i d->m d->d + COMPO 2.69099 4.42301 3.08956 2.63817 3.15095 3.22965 3.73733 3.00284 2.39844 2.59744 3.71648 2.92519 3.36709 2.96182 2.80106 2.40804 2.72823 2.85904 4.52259 3.39447 + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01040 4.96677 5.68911 0.61958 0.77255 0.00000 * + 1 4.31692 5.72794 5.52012 5.32473 4.32073 4.88041 5.82131 3.65784 5.06173 2.84902 0.23351 5.44418 5.43621 5.39765 5.11023 4.71024 4.69520 3.77421 6.18042 5.03499 1 M - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.29689 4.96677 1.38665 0.61958 0.77255 0.48576 0.95510 + 2 2.10907 4.99215 3.14045 1.93912 4.27111 3.57325 3.80272 2.96394 2.59507 3.28549 4.08941 3.11454 3.07395 2.94532 3.06247 2.14773 2.54072 3.35220 5.51607 4.17248 2 e - - - + 2.68595 4.42237 2.77532 2.73135 3.46285 2.40525 3.72507 3.29366 2.67753 2.69331 4.24702 2.90359 2.73752 3.18158 2.89813 2.37873 2.77532 2.98480 4.58489 3.61515 + 0.54411 1.37909 1.78480 0.53488 0.88126 1.00004 0.45865 + 3 2.81785 5.14391 2.87775 2.11425 4.43250 3.51531 3.79148 3.87530 2.58175 2.70905 4.23794 1.59148 2.86905 2.92909 3.05179 2.80758 3.06131 3.51133 5.63358 4.25979 5 n - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01655 4.50536 5.22771 0.61958 0.77255 1.18296 0.36582 + 4 2.78829 5.26960 2.35704 2.07710 4.59532 3.48150 3.73641 4.07451 2.51650 3.57017 4.32981 2.95381 3.91933 2.35672 3.01829 1.85067 2.36325 3.65267 5.71839 4.30438 6 s - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01655 4.50536 5.22771 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3.72752 5.06201 2.96161 2.74252 4.86057 5.38890 4.91234 4.97871 4.49239 4.49175 3.73457 4.29785 2.04524 372 F - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01112 4.90061 5.62296 0.61958 0.77255 0.64670 0.74186 + 214 2.82693 3.07584 4.08560 3.50633 2.79595 3.89241 4.17906 2.82487 2.73780 1.69531 3.47881 2.89594 4.26497 2.92120 3.65685 3.16554 3.05888 1.93430 4.97131 3.76877 373 l - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.16052 4.83508 1.96361 0.61958 0.77255 0.39687 1.11603 + 215 2.74054 4.29554 3.96474 3.38965 3.41927 3.79834 4.09499 2.06697 3.30448 2.49500 2.77509 2.83207 4.17576 3.56380 3.56270 2.40211 2.43442 1.90905 4.91345 3.70816 374 v - - - + 2.68695 4.42132 2.77500 2.73206 3.46027 2.40601 3.72488 3.29334 2.67780 2.69304 4.24813 2.90364 2.73733 3.18130 2.89763 2.37894 2.77400 2.98590 4.58600 3.61414 + 0.51548 0.90935 * 2.32753 0.10263 0.00000 * +// diff --git a/results/2026-10-30_mat_hmms/hmm/a2_no_Pichiaceae.hmm b/results/2026-10-30_mat_hmms/hmm/a2_no_Pichiaceae.hmm new file mode 100644 index 00000000..256caa9b --- /dev/null +++ b/results/2026-10-30_mat_hmms/hmm/a2_no_Pichiaceae.hmm @@ -0,0 +1,671 @@ +HMMER3/f [3.4 | Aug 2023] +NAME a2_no_Pichiaceae +LENG 216 +ALPH amino +RF no +MM no +CONS yes +CS no +MAP yes +DATE Fri Oct 30 06:51:30 2026 +COM [1] x +NSEQ 12 +EFFN 3.143555 +CKSUM 3731414313 +STATS LOCAL MSV -10.8279 0.70444 +STATS LOCAL VITERBI -11.8338 0.70444 +STATS LOCAL FORWARD -4.6146 0.70444 +HMM A C D E F G H I K L M N P Q R S T V W Y + m->m m->i m->d i->m i->i d->m d->d + COMPO 2.64968 4.43676 3.08355 2.66938 3.18566 3.20395 3.70316 2.98466 2.42430 2.56970 3.66272 2.90550 3.35783 2.96169 2.81801 2.42349 2.72948 2.86317 4.53702 3.44707 + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01140 4.87573 5.59807 0.61958 0.77255 0.00000 * + 1 4.16787 5.59083 5.37886 5.15985 4.17667 4.77442 5.67222 3.49453 4.89040 2.70283 0.27743 5.29054 5.31909 5.23091 4.95339 4.56312 4.53778 3.60340 6.04954 4.89208 1 M - - - + 2.68638 4.42245 2.77539 2.73102 3.46287 2.40532 3.72514 3.29311 2.67760 2.69304 4.24709 2.90366 2.73759 3.18166 2.89820 2.37855 2.77497 2.98538 4.58497 3.61523 + 0.83153 1.25608 1.27351 0.67057 0.71624 0.48576 0.95510 + 2 1.97913 4.87901 3.13008 1.84621 4.24140 3.51233 3.85292 3.62815 2.67255 3.26312 4.08340 3.13938 3.96722 3.01311 3.12834 2.00990 2.99260 2.55184 5.51573 4.18781 5 e - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01578 4.55257 5.27491 0.61958 0.77255 1.00911 0.45341 + 3 2.83831 5.17252 2.88958 2.09824 4.46272 3.53272 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3.71860 365 i - - - + 2.68676 4.42100 2.77518 2.73232 3.46064 2.40583 3.72461 3.29462 2.67799 2.69275 4.24798 2.90392 2.73752 3.18172 2.89734 2.37871 2.77363 2.98571 4.58585 3.61380 + 0.42623 1.05833 * 2.38474 0.09664 0.00000 * +// diff --git a/results/2026-10-30_mat_hmms/hmm/a2_no_Saccharomycetaceae.hmm b/results/2026-10-30_mat_hmms/hmm/a2_no_Saccharomycetaceae.hmm new file mode 100644 index 00000000..a7020b20 --- /dev/null +++ b/results/2026-10-30_mat_hmms/hmm/a2_no_Saccharomycetaceae.hmm @@ -0,0 +1,641 @@ +HMMER3/f [3.4 | Aug 2023] +NAME a2_no_Saccharomycetaceae +LENG 206 +ALPH amino +RF no +MM no +CONS yes +CS no +MAP yes +DATE Fri Oct 30 06:51:51 2026 +COM [1] x +NSEQ 8 +EFFN 2.074219 +CKSUM 1432680902 +STATS LOCAL MSV -10.5293 0.70499 +STATS LOCAL VITERBI -11.5376 0.70499 +STATS LOCAL FORWARD -4.7063 0.70499 +HMM A C D E F G H I K L M N P Q R S T V W Y + m->m m->i m->d i->m i->i d->m d->d + COMPO 2.67900 4.33096 3.06761 2.67456 3.15651 3.19199 3.77395 2.96260 2.42050 2.64227 3.71136 2.97003 3.37030 2.98606 2.76380 2.40440 2.76705 2.80352 4.67295 3.28110 + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01430 4.65067 5.37302 0.61958 0.77255 0.00000 * + 1 3.82317 5.27329 5.03713 4.76085 3.85837 4.52428 5.31802 3.13385 4.48657 2.38606 0.41721 4.92393 5.04148 4.83680 4.58490 4.21896 4.17296 3.22204 5.74891 4.56751 1 M - - - + 2.68630 4.42237 2.77532 2.73095 3.46282 2.40525 3.72507 3.29366 2.67753 2.69332 4.24702 2.90359 2.73752 3.18159 2.89813 2.37873 2.77495 2.98531 4.58489 3.61515 + 0.97012 1.34218 1.02252 0.53971 0.87448 0.48576 0.95510 + 2 2.16732 4.66791 3.06733 2.04434 3.99992 3.41322 3.77451 3.34441 2.62434 3.03027 3.87607 3.07603 3.87351 2.96197 3.06098 2.20559 2.88069 2.35937 5.32252 4.01836 4 e - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 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2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01430 4.65067 5.37302 0.61958 0.77255 0.48576 0.95510 + 204 2.82229 4.23282 4.53363 3.93995 1.65091 3.96266 4.24727 2.61025 3.77567 2.32286 2.64109 4.03456 4.32265 3.94478 3.86772 3.26547 2.37006 2.07412 4.78398 2.68797 304 f - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01430 4.65067 5.37302 0.61958 0.77255 0.48576 0.95510 + 205 2.68703 2.99595 3.50731 2.94038 2.79526 3.64877 3.89451 2.95170 2.38164 2.17810 3.54605 3.36075 2.92185 2.62058 3.24855 2.89045 2.91874 2.72222 5.02015 3.77818 305 l - - - + 2.68775 4.42427 2.77233 2.73236 3.46075 2.40646 3.72441 3.29242 2.67898 2.69374 4.24892 2.90399 2.73613 3.17837 2.89779 2.37897 2.77524 2.98544 4.58679 3.61476 + 0.82834 1.04112 1.55986 3.04426 0.04880 0.48576 0.95510 + 206 2.65064 4.75246 3.08822 2.57880 4.05762 2.47130 3.73467 3.45781 2.05193 2.44430 3.91699 3.06683 3.88557 2.90734 2.91344 2.20333 2.90494 3.14138 5.33626 4.03083 362 k - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01471 4.22636 * 0.61958 0.77255 0.00000 * +// diff --git a/results/2026-10-30_mat_hmms/hmm/alpha1.hmm b/results/2026-10-30_mat_hmms/hmm/alpha1.hmm new file mode 100644 index 00000000..529ff157 --- /dev/null +++ b/results/2026-10-30_mat_hmms/hmm/alpha1.hmm @@ -0,0 +1,584 @@ +HMMER3/f [3.4 | Aug 2023] +NAME alpha1 +LENG 187 +ALPH amino +RF no +MM no +CONS yes +CS no +MAP yes +DATE Fri Oct 30 06:49:53 2026 +COM [1] loco.py +NSEQ 12 +EFFN 1.725586 +CKSUM 4194392875 +STATS LOCAL MSV -10.6971 0.70619 +STATS LOCAL VITERBI -11.5475 0.70619 +STATS LOCAL FORWARD -4.8071 0.70619 +HMM A C D E F G H I K L M N P Q R S T V W Y + m->m m->i m->d i->m i->i d->m d->d + COMPO 2.62001 4.60844 3.02684 2.58557 3.25030 3.21206 3.70806 3.03270 2.44288 2.62141 3.76776 2.87444 3.37260 2.90964 2.84929 2.45142 2.80719 2.89752 4.01175 3.32389 + 2.68626 4.42259 2.77553 2.73134 3.46388 2.40546 3.72528 3.29307 2.67774 2.69344 4.24376 2.90353 2.73727 3.18144 2.89775 2.37888 2.77481 2.98552 4.58364 3.61481 + 0.34761 1.58361 2.42599 1.72837 0.19550 0.00000 * + 1 3.56976 4.92727 5.05207 4.53144 3.27225 4.72331 5.04747 2.45528 4.27527 1.44392 0.94834 4.77544 4.95395 4.47529 4.39156 4.12006 3.80936 2.60209 5.35710 4.22891 19 m - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01775 4.43626 5.15860 0.61958 0.77255 0.62158 0.77024 + 2 2.67437 4.59292 3.29930 2.73811 3.07227 2.56835 3.80989 3.14544 2.41016 2.82204 3.68592 2.76410 3.16422 3.04387 3.12854 2.80458 2.59564 2.59466 3.52378 3.87338 20 k - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 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4.58477 3.61503 + 0.10441 4.52303 2.42717 0.61958 0.77255 0.48576 0.95510 + 186 2.39615 4.89885 3.06489 2.51464 3.25749 2.58838 3.71521 3.57707 2.29255 3.17305 3.98112 3.03476 3.89729 2.32016 2.97112 2.35962 2.60945 3.24504 5.40897 4.06974 263 k - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.16818 4.43637 1.94519 0.61958 0.77255 0.62142 0.77042 + 187 2.39916 4.76953 3.08150 2.27346 3.99933 3.48432 3.70186 3.39476 2.24750 2.62119 3.85608 3.04194 3.87961 2.86994 2.95652 2.10515 2.87947 2.71400 5.29375 3.97775 264 s - - - + 2.68752 4.42146 2.77421 2.73225 3.46068 2.40662 3.72561 3.29235 2.67876 2.69238 4.24534 2.90162 2.73813 3.18079 2.89882 2.37796 2.77506 2.98622 4.58348 3.61479 + 0.50201 0.92966 * 3.11978 0.04517 0.00000 * +// diff --git a/results/2026-10-30_mat_hmms/hmm/alpha2.hmm b/results/2026-10-30_mat_hmms/hmm/alpha2.hmm new file mode 100644 index 00000000..8f0daf59 --- /dev/null +++ b/results/2026-10-30_mat_hmms/hmm/alpha2.hmm @@ -0,0 +1,653 @@ +HMMER3/f [3.4 | Aug 2023] +NAME alpha2 +LENG 210 +ALPH amino +RF no +MM no +CONS yes +CS no +MAP yes +DATE Fri Oct 30 06:48:40 2026 +COM [1] loco.py +NSEQ 25 +EFFN 2.182007 +CKSUM 2877813333 +STATS LOCAL MSV -10.8470 0.70477 +STATS LOCAL VITERBI -11.7417 0.70477 +STATS LOCAL FORWARD -4.5648 0.70477 +HMM A C D E F G H I K L M N P Q R S T V W Y + m->m m->i m->d i->m i->i d->m d->d + COMPO 2.74938 4.59707 3.04694 2.55115 3.61295 3.37563 3.66630 2.86364 2.40826 2.39116 3.64354 2.80751 3.47197 2.85025 2.75796 2.52397 2.79222 2.87785 4.34731 3.66066 + 2.68626 4.42233 2.77528 2.73122 3.46362 2.40521 3.72503 3.29362 2.67738 2.69363 4.24410 2.90355 2.73748 3.18155 2.89809 2.37880 2.77504 2.98527 4.58485 3.61511 + 0.18968 1.78596 5.26996 0.69193 0.69437 0.00000 * + 1 2.89631 4.42488 4.17900 3.62000 3.50075 3.94514 4.27190 2.30332 3.08418 2.43712 1.17036 3.88245 4.33317 3.73977 3.60532 3.01615 3.14062 2.53090 5.06012 3.86227 4 m - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01438 4.64471 5.36705 0.61958 0.77255 0.48576 0.95510 + 2 2.84615 5.29101 2.94239 2.49605 4.64270 3.01223 3.79552 4.11246 2.12805 3.60952 4.37398 1.81022 3.98565 2.61869 3.00590 1.86900 3.08601 3.69495 5.75412 4.35928 5 n - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01438 4.64471 5.36705 0.61958 0.77255 0.48576 0.95510 + 3 2.83764 5.29491 3.10391 2.31024 4.65435 3.59434 2.86964 4.11494 1.56012 3.58429 4.34037 3.06619 3.98198 2.58711 2.24588 2.46983 2.82893 3.69516 5.69904 4.32934 6 k - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01438 4.64471 5.36705 0.61958 0.77255 0.48576 0.95510 + 4 2.76512 4.49919 3.10482 3.04268 3.60660 3.73108 3.14268 1.45534 2.99220 2.47394 3.59969 3.45434 4.11273 2.87899 3.34679 2.97968 2.99664 2.77011 5.05444 3.35660 7 i - - - + 2.68662 4.42191 2.77490 2.73115 3.46349 2.40539 3.72484 3.29357 2.67721 2.69357 4.24627 2.90362 2.73760 3.18167 2.89793 2.37799 2.77563 2.98536 4.58521 3.61547 + 0.29117 1.96916 2.18001 2.17318 0.12083 0.48576 0.95510 + 5 2.56441 4.75874 3.23980 2.71359 4.08449 3.52490 3.86190 3.48204 2.69592 2.71789 3.95879 3.19020 1.82828 2.76880 3.12653 2.24979 2.51039 3.16722 5.39899 4.09909 30 p - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01615 4.53000 5.25235 0.61958 0.77255 0.67985 0.70662 + 6 2.70630 4.40350 4.53914 3.06749 3.60448 4.21688 4.59045 1.22825 3.87141 2.20630 3.52084 4.20523 4.55873 4.11594 4.06937 3.52900 3.24317 1.56751 5.25175 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4.52144 4.13757 3.94142 4.98050 3.29518 4.93227 4.41069 4.00791 4.12641 4.93192 3.88068 4.11217 4.24743 4.24191 0.61493 3.13032 3.71776 6.32342 5.16119 280 S - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 188 3.61647 5.52369 3.48024 3.46903 5.04131 3.91997 4.86624 5.00010 3.88751 4.55661 5.55560 0.31472 4.62722 4.19522 4.22337 3.68593 4.05173 4.53097 6.23702 4.97689 281 N - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 189 3.71840 5.72593 4.34904 3.43720 5.35627 4.22036 3.97721 4.61201 1.51089 3.93434 4.83766 3.74110 4.54067 3.10529 0.73279 3.69197 3.81308 4.31172 5.86710 4.81619 282 r - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 190 4.10957 5.76424 4.69145 4.11284 5.33378 4.36061 4.69331 4.99532 2.95354 4.36811 5.39503 4.38986 4.87006 3.91988 0.26527 4.19032 4.35162 4.69446 6.12832 5.15857 283 R - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 191 4.10957 5.76424 4.69145 4.11284 5.33378 4.36061 4.69331 4.99532 2.95354 4.36811 5.39503 4.38986 4.87006 3.91988 0.26527 4.19032 4.35162 4.69446 6.12832 5.15857 284 R - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 192 3.91062 5.74998 4.13258 3.67902 5.32980 4.23072 4.46864 4.83770 0.33394 4.25479 5.23032 4.04424 4.72171 3.65621 2.92195 3.93680 4.13073 4.52270 6.09799 5.06611 285 K - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 193 2.87788 5.40088 2.33882 1.35298 4.73242 3.53446 3.80394 4.22286 2.58693 3.69936 4.45536 2.99718 3.98550 2.47282 2.74137 2.31855 3.11822 3.78631 5.83331 4.40632 286 e - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 194 3.49328 5.61492 4.01746 3.21995 5.18467 4.08000 3.92206 4.47396 0.82849 3.84138 4.71070 3.58730 4.41652 3.04508 1.61002 3.46916 3.19523 4.14655 5.82493 4.70846 287 k - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 195 2.74822 5.08826 3.06272 2.51327 4.37790 3.11043 3.46461 3.30210 2.17556 3.37298 4.15391 2.46740 3.94069 2.86882 2.98379 2.00087 2.15518 3.45084 5.56641 4.19859 288 s - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 196 2.74211 4.51228 3.54527 2.71030 3.65861 3.70090 3.94913 2.39060 2.94208 2.20149 3.61414 2.95401 3.38223 3.25275 3.31479 2.27097 2.39811 2.35581 5.08928 3.84564 289 l - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 197 2.74835 5.19968 3.01876 2.30518 4.52327 3.52892 3.31753 3.99182 2.09638 3.49410 4.24678 3.00591 3.11307 2.55377 2.68596 2.43058 1.98786 3.57889 5.64384 3.77060 290 t - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 198 3.22773 4.53662 5.10029 4.55523 3.31941 4.56026 5.02007 1.26826 4.42732 2.43479 3.60768 4.67402 3.88157 4.62293 4.54267 3.91090 3.47556 1.04157 5.50483 4.30914 291 v - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 199 2.41512 4.97826 2.40002 2.68578 4.44623 3.51513 3.96501 3.86233 2.80573 2.93743 4.28833 3.18311 4.02588 3.12351 3.27316 1.19494 3.09149 3.48244 5.70311 4.35739 292 s - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01452 4.63521 5.35756 0.61958 0.77255 0.58486 0.81460 + 200 2.74420 4.72264 3.31648 2.75899 3.31083 3.62754 3.86177 3.30288 2.37704 2.46106 3.82163 3.24013 2.27134 3.07358 3.15789 2.06109 2.57722 3.03186 5.27566 3.99038 293 s - - - + 2.68619 4.42226 2.77520 2.73124 3.46331 2.40513 3.72495 3.29355 2.67741 2.69355 4.24690 2.90347 2.73740 3.18147 2.89801 2.37887 2.77520 2.98519 4.58477 3.61504 + 0.04136 3.32980 5.35756 0.45682 1.00319 0.58486 0.81460 + 201 2.79220 5.24700 2.35682 1.44431 4.56389 3.53088 3.75301 3.65359 2.41365 3.11095 4.29980 3.00648 3.94638 2.86312 3.00893 2.31530 3.02694 3.62238 5.69549 4.29393 295 e - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03741 4.63521 3.61153 0.61958 0.77255 0.58486 0.81460 + 202 3.65770 4.88086 5.62020 5.08564 3.59676 5.12143 5.57834 1.33091 4.97275 0.96804 3.35024 5.26032 5.26360 5.02022 5.02356 4.51733 3.89076 1.79404 5.74460 4.68880 296 l - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03980 4.61266 3.53740 0.61958 0.77255 0.62364 0.76785 + 203 2.21879 4.86229 3.16955 2.42511 4.08346 3.57266 2.99403 3.14607 2.40473 2.53026 3.93837 3.12137 3.96137 2.94547 2.76952 2.15571 2.95790 2.85558 5.37696 4.05728 297 s - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01523 4.58809 5.31044 0.61958 0.77255 0.66331 0.72390 + 204 2.32946 5.30723 2.22054 2.08540 4.64297 3.50521 3.73896 4.13084 2.50406 3.60910 4.35563 2.35407 3.32691 2.20741 3.00654 2.48076 3.02930 3.69388 5.74260 4.32278 298 e - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01523 4.58809 5.31044 0.61958 0.77255 0.66331 0.72390 + 205 2.81593 4.74360 5.22247 4.67791 3.51458 4.73581 5.16116 1.47600 4.53016 0.92685 3.31749 4.84553 4.98418 4.66001 4.62227 4.09969 3.67624 2.21641 5.50855 4.40985 299 l - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01523 4.58809 5.31044 0.61958 0.77255 0.66331 0.72390 + 206 3.15984 4.53075 4.84783 3.56739 2.81443 4.35817 4.67248 2.16105 4.11695 0.89160 3.21199 4.41788 4.64885 4.25273 4.21484 3.67478 3.38694 2.18229 5.13131 3.99715 300 l - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01523 4.58809 5.31044 0.61958 0.77255 0.66331 0.72390 + 207 2.81133 5.11921 3.09301 2.56550 4.42118 3.57823 3.76984 3.85807 2.47054 2.83690 4.19844 1.57913 3.98195 2.67068 2.46292 2.36558 3.04177 3.49535 5.58489 4.23769 301 n - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.11462 4.58809 2.32153 0.61958 0.77255 0.66331 0.72390 + 208 2.70750 5.09198 2.75736 2.43224 4.39446 2.29349 3.68385 3.84648 2.13420 2.92834 4.15191 2.97072 3.88244 2.23774 2.90821 2.69308 2.56242 3.46093 5.55446 4.17650 302 k - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.01768 4.44005 5.16239 0.61958 0.77255 0.79936 0.59714 + 209 2.75553 5.27039 2.38019 2.04589 4.60421 2.84576 3.69671 4.09207 2.10247 3.57064 4.31979 2.71031 3.88605 2.53664 2.96808 2.07466 2.99265 3.65610 5.70480 4.28392 303 e - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.24204 4.44005 1.59366 0.61958 0.77255 0.79936 0.59714 + 210 2.60564 4.74901 3.00216 2.48030 4.01659 3.41207 3.65193 3.42730 2.08343 3.04007 3.86657 2.98326 2.64134 2.81518 2.84898 2.43976 2.60970 3.11002 5.28532 3.46332 304 k - - - + 2.68634 4.42013 2.77542 2.73118 3.46358 2.40521 3.72516 3.29262 2.67709 2.69338 4.24742 2.90382 2.73757 3.18167 2.89853 2.37875 2.77511 2.98504 4.58529 3.61500 + 0.58475 0.81474 * 1.88253 0.16512 0.00000 * +// diff --git a/results/2026-10-30_mat_hmms/inventory.py b/results/2026-10-30_mat_hmms/inventory.py new file mode 100644 index 00000000..b8a2f873 --- /dev/null +++ b/results/2026-10-30_mat_hmms/inventory.py @@ -0,0 +1,30 @@ +"""Curated MAT proteins in db/ (Saccharomycotina only) by gene name -> class a1 / a2 / alpha1 / alpha2.""" +import re, glob, collections, sys +ROOT = "../../db/Ascomycota" +def cls(n): + n = n.lower().replace("mtl", "mat") + m = re.fullmatch(r"mat(a|alpha)([12])", n) + return (m.group(1) + m.group(2)) if m else None +out = []; cnt = collections.Counter() +for f in sorted(glob.glob(ROOT + "/*/*/proteins.faa")): + parts = f.split("/") + order, rec = parts[-3], parts[-2] + if order in ("Pezizales", "Eurotiales", "Hypocreales", "Helotiales", "Sordariales", "Onygenales", "Glomerellales", "Diaporthales", "Mycosphaerellales", "Microascales", "Lecanorales", "Ophiostomatales", "Xylariales", "Capnodiales", "Pleosporales", "Chaetothyriales", "Sordariomycetes"): continue + name = None; seq = [] + for l in open(f): + if l.startswith(">"): + if name: out.append((rec, name, "".join(seq))) + m = re.search(r"name=([^|]+)", l); name = m.group(1) if m else l[1:].split("|")[-1].strip(); seq = [] + else: seq.append(l.strip()) + if name: out.append((rec, name, "".join(seq))) +keep = [] +for rec, name, s in out: + c = cls(name) + if c: keep.append((rec, name, c, s)); cnt[(rec.split("_")[0], c)] += 1 +with open("data/curated_mat_proteins.faa", "w") as fh: + for rec, name, c, s in keep: fh.write(f">{rec}|{name}|{c}\n{s}\n") +orders = collections.Counter() +for rec, name, c, s in keep: orders[c] += 1 +print(orders); print(sorted({r.split('_')[0] for r, *_ in keep})) +for rec, name, c, s in keep: + if c in ("a1", "alpha2", "a2"): print(c, rec, name, len(s)) diff --git a/results/2026-10-30_mat_hmms/loco.py b/results/2026-10-30_mat_hmms/loco.py new file mode 100644 index 00000000..e5bfd0f4 --- /dev/null +++ b/results/2026-10-30_mat_hmms/loco.py @@ -0,0 +1,62 @@ +"""Leave-one-clade-out and leave-one-species-out evaluation of a1 vs alpha2 (and a2, alpha1) profiles; builds the final profiles. +Run on the HPCC (mafft module): python loco.py""" +import subprocess, sys, tempfile, collections, io, json +import pyhmmer +from pyhmmer.easel import Alphabet, TextSequence, TextMSA, DigitalSequenceBlock +from pyhmmer.plan7 import Builder, Background +alpha = Alphabet.amino() +meta = [l.rstrip("\n").split("\t") for l in open("data/set_meta.tsv")][1:] +seqs = {} # id -> (class, species, clade, seq) +fa = {} +for c in ("a1", "alpha2", "a2", "alpha1"): + name = None + for l in open(f"data/set_{c}.faa"): + if l.startswith(">"): name = l[1:].strip(); fa[name] = "" + elif name: fa[name] += l.strip() +for c, sp, cl, src, ident, ln in meta: + seqs[ident.replace(" ", "_")] = (c, sp, cl, fa[ident.replace(" ", "_")]) +def mafft(items): + with tempfile.NamedTemporaryFile("w", suffix=".faa", delete=False) as fh: + for i, s in items: fh.write(f">{i}\n{s}\n") + fh.flush() + out = subprocess.run(["mafft", "--localpair", "--maxiterate", "1000", "--quiet", fh.name], capture_output=True, text=True) + if out.returncode: sys.exit("mafft failed: " + out.stderr[-500:]) + out = out.stdout + d = {}; n = None + for l in out.split("\n"): + if l.startswith(">"): n = l[1:]; d[n] = "" + elif n: d[n] += l.strip() + return d +def build(name, items): + aln = mafft(items) + msa = TextMSA(name=name.encode(), sequences=[TextSequence(name=i.encode(), sequence=s.replace(".", "-")) for i, s in aln.items()]).digitize(alpha) + hmm, _, _ = Builder(alpha, seed=42).build_msa(msa, Background(alpha)) + return hmm +def score(hmm, seq): + q = TextSequence(name=b"q", sequence=seq).digitize(alpha) + best = 0.0 + for hits in pyhmmer.hmmsearch([hmm], [q], E=1e6, Z=1000): + for h in hits: best = max(best, h.score) + return best +CL = ["Saccharomycetaceae", "Serinales", "Pichiaceae", "Dipodascales", "other"] +def cls_items(c, exclude=lambda v: False): + return [(i, v[3]) for i, v in seqs.items() if v[0] == c and not exclude(v)] +res = [] +for scheme in ("clade", "species"): + groups = sorted({(v[2] if scheme == "clade" else v[1]) for v in seqs.values() if v[0] in ("a1", "alpha2")}) + for g in groups: + ex = (lambda v, g=g: (v[2] if scheme == "clade" else v[1]) == g) + ha1 = build("a1", cls_items("a1", ex)); hal2 = build("alpha2", cls_items("alpha2", ex)) + n1 = len(cls_items("a1", ex)); n2 = len(cls_items("alpha2", ex)) + for i, v in seqs.items(): + if v[0] in ("a1", "alpha2") and ex(v): + s1, s2 = score(ha1, v[3]), score(hal2, v[3]) + res.append((scheme, g, i, v[0], v[1], round(s1, 1), round(s2, 1), "a1" if s1 > s2 else "alpha2", n1, n2)) +with open("data/loco.tsv", "w") as fh: + fh.write("scheme\theldout\tid\ttrue\tspecies\ta1_score\talpha2_score\tcall\tn_a1_train\tn_alpha2_train\n") + for r in res: fh.write("\t".join(map(str, r)) + "\n") +import os; os.makedirs("hmm", exist_ok=True) +for c in ("a1", "alpha2", "a2", "alpha1"): + h = build(c, cls_items(c)) + with open(f"hmm/{c}.hmm", "wb") as fh: h.write(fh) + print(c, len(cls_items(c)), "sequences, model length", h.M) diff --git a/results/2026-10-30_mat_hmms/loco.slurm b/results/2026-10-30_mat_hmms/loco.slurm new file mode 100644 index 00000000..fd85ad4c --- /dev/null +++ b/results/2026-10-30_mat_hmms/loco.slurm @@ -0,0 +1,9 @@ +#!/bin/bash +#SBATCH -p exfab -A exfab -c 2 --mem 8G -t 02:00:00 -J mat-loco +#SBATCH -o /bigdata/stajichlab/jstajich/projects/MATPredict/logs/mat_loco_%j.log +set -euo pipefail +set +eu; source /etc/profile 2>/dev/null; module load mafft/7.505; set -eu; unset MAFFT_BINARIES +export PATH=/bigdata/stajichlab/jstajich/projects/MATPredict/.pixi/envs/default/bin:$PATH +cd /bigdata/stajichlab/jstajich/projects/MATPredict/results/2026-10-30_mat_hmms +python loco.py +touch LOCO_DONE diff --git a/results/2026-10-30_mat_hmms/make_tests.py b/results/2026-10-30_mat_hmms/make_tests.py new file mode 100644 index 00000000..f8ae5a70 --- /dev/null +++ b/results/2026-10-30_mat_hmms/make_tests.py @@ -0,0 +1,39 @@ +"""Candidate proteins to score, and six-frame ORFs of the B. californica region 620,300-620,800 (unmodelled homeodomain).""" +import re, itertools +CODON = {a + b + c: aa for (a, b, c), aa in zip(itertools.product("TCAG", repeat=3), "FFLLSSSSYY**CC*WLLLLPPPPHHQQRRRRIIIMTTTTNNKKSSRRVVVVAAAADDEEGGGG")} +def rc(s): return s[::-1].translate(str.maketrans("ACGTacgt", "TGCAtgca")) +def tr(s): return "".join(CODON.get(s[i:i + 3].upper(), "X") for i in range(0, len(s) - 2, 3)) +def fasta(path): + d = {}; n = None + for l in open(path): + if l.startswith(">"): n = l[1:].split()[0]; d[n] = "" + elif n: d[n] += l.strip() + return d +out = [] +cur = fasta("data/curated_mat_proteins.faa") +for k, v in cur.items(): + if "4909_cbs-573_MATpk_MATa|MATa2" in k: out.append(("Pkud_MATa2_control", v)) +pf = fasta("../2026-10-30_petasospora/data/models_proteins.faa") +for k, v in pf.items(): + if k.startswith("MATa_JOY008|MATa") or k.startswith("MATalpha_TBRC4498|MATalpha1"): out.append(("PF_" + k.split("|")[0].split("_")[1] + "_" + k.split("|")[1], v)) +bc = fasta("../2026-10-30_barnettozyma/models_proteins.faa") +for k, v in bc.items(): + if k in ("BC_MATa2", "BC_MATa1", "BC_MATalpha1"): out.append((k, v)) +ss = fasta("/Users/jstajich/projects/MATPredict/results/2026-10-29_ss_records/data/longest_orfs.pep") +for k in ("SS.1982.2.p3", "SS.1982.1.p3", "SS.1982.2.p4", "SS.1982.2.p5", "SS.2002.2.p2", "SS.2001.1.p4", "SS.2002.1.p2"): + out.append((k, ss[k].rstrip("*"))) +# B. californica unmodelled region +g = fasta("../2026-10-30_barnettozyma/data/GCA_023627795.1_JAMAIE010000005.1_589225-655853.fa") +seq = next(iter(g.values())); OFF = 589225 +for a, b in ((620000, 621100),): + sub = seq[a - OFF:b - OFF + 1] + for strand, s in (("+", sub), ("-", rc(sub))): + for f in range(3): + p = tr(s[f:]) + pos = 0 + for seg in p.split("*"): + if len(seg) >= 50: out.append((f"BCreg_{strand}{f}_{pos}", seg)) + pos += len(seg) + 1 +with open("data/test_proteins.faa", "w") as fh: + for n, s in out: fh.write(f">{n}\n{s}\n") +print(len(out)) diff --git a/results/2026-10-30_mat_hmms/pfam_scan.py b/results/2026-10-30_mat_hmms/pfam_scan.py new file mode 100644 index 00000000..3365da5c --- /dev/null +++ b/results/2026-10-30_mat_hmms/pfam_scan.py @@ -0,0 +1,21 @@ +"""Pfam HMG-box and homeodomain models against the test proteins (all hits, E <= 10, with the Pfam GA bit cutoff).""" +import pyhmmer +from pyhmmer.easel import SequenceFile, Alphabet +PF = "/Users/jstajich/db/funannotate/Pfam-A.hmm" +WANT = {"HMG_box", "HMG_box_2", "HMG_box_5", "HMGL-like", "Homeodomain", "Homeobox_KN", "MATalpha_HMGbox", "SoxE", "Sox17_18_mid"} +alpha = Alphabet.amino() +with SequenceFile("data/test_proteins.faa", digital=True, alphabet=alpha) as sf: q = sf.read_block() +models = [] +with pyhmmer.plan7.HMMFile(PF) as hf: + for h in hf: + if str(h.name) in WANT: models.append(h) +print("models", [str(m.name) for m in models]) +rows = {} +for hits in pyhmmer.hmmsearch(models, q, E=10): + m = str(hits.query.name); ga = hits.query.cutoffs.gathering[0] if hits.query.cutoffs.gathering else None + for h in hits: + rows.setdefault(str(h.name), []).append(f"{m} {h.score:.1f}/{ga:.1f}" if ga else f"{m} {h.score:.1f}") +with open("data/pfam_hits.tsv", "w") as fh: + for k in [str(s.name) for s in q]: + fh.write(k + "\t" + "; ".join(rows.get(k, [])) + "\n") +print(open("data/pfam_hits.tsv").read()) diff --git a/results/2026-10-30_mat_hmms/score_models.py b/results/2026-10-30_mat_hmms/score_models.py new file mode 100644 index 00000000..8e33f70b --- /dev/null +++ b/results/2026-10-30_mat_hmms/score_models.py @@ -0,0 +1,31 @@ +"""Score S. schoenii candidate protein models (stdin-free; edit MODELS) against the a1, alpha2, a2 profiles.""" +import pyhmmer +from pyhmmer.easel import Alphabet, TextSequence +seq = "".join(l.strip() for l in open("../2026-10-30_saccharomycopsis/chrIV_463k-542k.fa") if not l.startswith(">")).upper() +W = 463000 +from itertools import product +bases = "TCAG"; aa = "FFLLSSSSYY**CC*WLLLLPPPPHHQQRRRRIIIMTTTTNNKKSSRRVVVVAAAADDEEGGGG" +codon = {a + b + c: aa[i] for i, (a, b, c) in enumerate(product(bases, repeat=3))} +def sub(a, b): return seq[a - W:b - W + 1] +def rc(s): return s[::-1].translate(str.maketrans("ACGTN", "TGCAN")) +def tr(s): return "".join(codon.get(s[i:i + 3], "X") for i in range(0, len(s) - 2, 3)) +def prot(exons, strand): + s = "".join(sub(a, b) for a, b in sorted(exons)); return tr(s if strand == "+" else rc(s)) +MODELS = { + "left_a1_1exon_ATG471961-472464": ([(471961, 472464)], "+"), + "left_a1_p5_2exon_103aa": ([(471961, 472227), (472445, 472489)], "+"), + "left_a1_agent_471889-472461": ([(471889, 472461)], "+"), + "right_a1_536074-536634_minus": ([(536074, 536634)], "-"), + "right_a1_3exon_211aa": ([(536046, 536090), (536296, 536634), (536693, 536944)], "-"), + "left_alpha2_470349-470951": ([(470349, 470951)], "+"), + "right_a2_537367-538167": ([(537367, 538167)], "+"), +} +alpha = Alphabet.amino() +hmms = {n: next(pyhmmer.plan7.HMMFile(f"hmm/{n}.hmm")) for n in ("a1", "alpha2", "a2", "alpha1")} +for name, (ex, st) in MODELS.items(): + p = prot(ex, st); q = TextSequence(name=name.encode(), sequence=p.replace("*", "")).digitize(alpha) + out = [] + for hn, h in hmms.items(): + hits = list(pyhmmer.hmmsearch(h, [q], E=10))[0] + out.append(f"{hn} {hits[0].score:.0f}" if len(hits) else f"{hn} -") + print(name, len(p.rstrip('*')), "aa", "stop" if p.endswith("*") else "nostop", "start", p[0], " | ", " ".join(out)) diff --git a/results/2026-10-30_mat_hmms/score_tests.py b/results/2026-10-30_mat_hmms/score_tests.py new file mode 100644 index 00000000..11cdb058 --- /dev/null +++ b/results/2026-10-30_mat_hmms/score_tests.py @@ -0,0 +1,46 @@ +"""Score the test proteins with the new a1 / alpha2 / a2 / alpha1 profiles and the Saccharomycotina MATa2 profile, against a background +of the S. schoenii proteome (TransDecoder ORFs, a non-MAT-biased Saccharomycotina set).""" +import sys, pyhmmer +from pyhmmer.easel import Alphabet, TextSequence +alpha = Alphabet.amino() +HM = {n: f"hmm/{n}.hmm" for n in ("a1", "alpha2", "a2", "alpha1", "a2_no_Pichiaceae", "a2_no_Saccharomycetaceae", "a2_no_Serinales")} +HM["sacch_a2(existing)"] = "../../db/Ascomycota/classifiers/MATa2_saccharomycotina/MATa2_saccharomycotina.hmm" +import os +hmms = {} +for n, p in HM.items(): + if os.path.exists(p): + with pyhmmer.plan7.HMMFile(p) as hf: hmms[n] = hf.read() +def fa(path): + d = {}; n = None + for l in open(path): + if l.startswith(">"): n = l[1:].split()[0]; d[n] = "" + elif n: d[n] += l.strip().rstrip("*") + return d +test = fa("data/test_proteins.faa") +bg = {k: v for k, v in fa("/Users/jstajich/projects/MATPredict/results/2026-10-29_ss_records/data/transcripts.fa.transdecoder.pep").items() if len(v) >= 60} +print("background proteins", len(bg), file=sys.stderr) +def block(d): return [TextSequence(name=k.encode(), sequence=v).digitize(alpha) for k, v in d.items()] +def run(h, d): + out = {} + for hits in pyhmmer.hmmsearch([h], block(d), E=1e9, domE=1e9, incE=1e9, incdomE=1e9, Z=3067, F1=1.0, F2=1.0, F3=1.0, bias_filter=False): + for x in hits: out[str(x.name)] = max(out.get(str(x.name), -9), x.score) + return out +BG = {n: run(h, bg) for n, h in hmms.items()} +T = {n: run(h, test) for n, h in hmms.items()} +print({n: len(BG[n]) for n in hmms}, file=sys.stderr) +cols = list(hmms) +with open("data/test_scores.tsv", "w") as fh: + fh.write("protein\tlen\t" + "\t".join(cols) + "\n") + for k, v in test.items(): + fh.write(f"{k}\t{len(v)}\t" + "\t".join(f"{T[n].get(k, 0):.1f}" for n in cols) + "\n") + fh.write("BACKGROUND_max\t\t" + "\t".join(f"{max(BG[n].values()):.1f}" for n in cols) + "\n") + fh.write("BACKGROUND_p99\t\t" + "\t".join(f"{sorted(BG[n].values())[int(0.99*len(BG[n]))]:.1f}" for n in cols) + "\n") + # rank of each test protein among the background for the a2 profiles + for n in cols: + pass +rank = {} +with open("data/test_rank.tsv", "w") as fh: + fh.write("protein\t" + "\t".join(f"{n}:bits(bg proteins >=)" for n in cols) + "\n") + for k in test: + fh.write(k + "\t" + "\t".join(f"{T[n].get(k,0):.0f}({sum(1 for s in BG[n].values() if s >= T[n].get(k,0))})" for n in cols) + "\n") +print(open("data/test_scores.tsv").read()); print(open("data/test_rank.tsv").read()) diff --git a/results/2026-10-30_mat_hmms/ss_a2_scan.py b/results/2026-10-30_mat_hmms/ss_a2_scan.py new file mode 100644 index 00000000..ee5768f1 --- /dev/null +++ b/results/2026-10-30_mat_hmms/ss_a2_scan.py @@ -0,0 +1,40 @@ +"""Six-frame scan of the whole S. schoenii genome (GCA_054953955.1) for MATa2-like (HMG-like) and alpha2-like / a1-like ORFs (stop-to-stop segments >= 60 aa, +default HMMER filters, E <= 1e-2). Lists hits within 15 kb of a MAT homeodomain/alpha-box hit as 'cassette neighbours'.""" +import itertools, pyhmmer, sys, collections +from pyhmmer.easel import Alphabet, TextSequence +CODON = {a + b + c: aa for (a, b, c), aa in zip(itertools.product("TCAG", repeat=3), "FFLLSSSSYY**CC*WLLLLPPPPHHQQRRRRIIIMTTTTNNKKSSRRVVVVAAAADDEEGGGG")} +def rc(s): return s[::-1].translate(str.maketrans("ACGTacgt", "TGCAtgca")) +def tr(s): return "".join(CODON.get(s[i:i + 3].upper(), "X") for i in range(0, len(s) - 2, 3)) +g = {}; n = None +for l in open("work/ss_genome.fa"): + if l.startswith(">"): n = l[1:].split()[0]; g[n] = [] + else: g[n].append(l.strip()) +g = {k: "".join(v) for k, v in g.items()} +alpha = Alphabet.amino(); H = {} +for n in ("a2", "a2_no_Pichiaceae", "a2_no_Saccharomycetaceae", "sacch_a2", "a1", "alpha2", "alpha1"): + p = "../../db/Ascomycota/classifiers/MATa2_saccharomycotina/MATa2_saccharomycotina.hmm" if n == "sacch_a2" else f"hmm/{n}.hmm" + with pyhmmer.plan7.HMMFile(p) as hf: H[n] = hf.read() +segs = [] +for c, s in g.items(): + if len(s) < 200000: continue # chromosomes only + for strand, t in (("+", s), ("-", rc(s))): + for f in range(3): + p = tr(t[f:]); pos = 0 + for seg in p.split("*"): + if len(seg) >= 60: + n0 = f + 3 * pos; n1 = n0 + 3 * len(seg) - 1 + a, b = (n0 + 1, n1 + 1) if strand == "+" else (len(s) - n1, len(s) - n0) + segs.append((f"{c}:{a}-{b}({strand})", seg)) + pos += len(seg) + 1 +print("segments", len(segs), file=sys.stderr) +q = [TextSequence(name=k.encode(), sequence=v).digitize(alpha) for k, v in segs] +hits = collections.defaultdict(dict) +for n, h in H.items(): + for th in pyhmmer.hmmsearch([h], q, E=1e-2, cpus=4): + for x in th: hits[str(x.name)][n] = round(x.score, 1) +rows = sorted(hits.items(), key=lambda kv: -max(kv[1].get("a2", 0), kv[1].get("sacch_a2", 0))) +with open("data/ss_genome_scan.tsv", "w") as fh: + fh.write("segment\t" + "\t".join(H) + "\n") + for k, v in rows: fh.write(k + "\t" + "\t".join(str(v.get(n, "")) for n in H) + "\n") +print("top a2 / sacch_a2:") +for k, v in rows[:12]: print(k, v) diff --git a/results/2026-10-30_saccharomycopsis/README.md b/results/2026-10-30_saccharomycopsis/README.md index f46baf6c..f52c95d9 100644 --- a/results/2026-10-30_saccharomycopsis/README.md +++ b/results/2026-10-30_saccharomycopsis/README.md @@ -3,16 +3,18 @@ Curator decisions (2026-10-30): draft records from our own gene models (tier 3); a scope-only family per genus, as for MATkp, MATog and MATpk. - **Family** `MATss` (scope: the species *S. schoenii*, taxid 44080, narrowed from the genus on 2026-10-30 because *S. lassenensis* is heterothallic, so other species are expected to differ; scope-only, never searched on a phylum fallback) in `db/Ascomycota/order.yml`. -- **Records** (candidates, `needs_review`): `44080_gdd12_MATss_MATalpha` (left sub-cluster: MATalpha1, MATalpha2, sla2) and `44080_gdd12_MATss_MATa` (right palindromic cassette: sla2, MATa1). Homothallic. Strain GDD12, assembly GCA_054953955.1, chromosome IV CM143129.1. +- **Records** (candidates, `needs_review`): `44080_gdd12_MATss_MATa-MATalpha` (left sub-cluster: MATalpha2, MATalpha1, MATa1, sla2) and `44080_gdd12_MATss_MATa` (right palindromic cassette: sla2, MATa1, MATa2). Homothallic. Strain GDD12, assembly GCA_054953955.1, chromosome IV CM143129.1. - **Evidence:** Kriti et al., G3 2026 ([10.1093/g3journal/jkag067](https://doi.org/10.1093/g3journal/jkag067), PMID 41849659) for the SLA2-MAT architecture and locus spans; Dost et al., Microbiol Res 2024 ([10.1016/j.micres.2024.127691](https://doi.org/10.1016/j.micres.2024.127691), PMID 38492364) for homothallism. **Gene coordinates are ours**: the authors' GTF is not public. Models, method and limits: `../2026-10-29_ss_records/README.md`. - **Genetic code:** NCBI table 12 (CUG = Ser); every gene carries `transl_table: 12`. - **Checks:** `make_records.py` re-extracts every CDS from the window fasta (stop codon, no internal stop, expected length); `curate-db propose` passed; `curate-db validate` under Slurm (`validate.slurm`, job 31445666) gave `taxonomy_current` true. No protein accessions, so no per-gene sequence match. +## Correction after the a1/alpha2/a2 profiles (2026-10-30, results/2026-10-30_mat_hmms) +The first drafts named the genes by position after the paper. The new profiles (supporting evidence only) showed that the "HMG-like" ORF at 470,349-470,951 is **MATalpha2** (alpha2 73 bits, a1 12), the left homeodomain is **MATa1** (a1 86, alpha2 29), and the real **MATa2** sits in the right cassette at 537,379-538,170 (a2 78). The records were rebuilt accordingly; the left record now holds both an alpha pair and an a1 beside SLA2, so its id is `..._MATa-MATalpha`. The spliced two-exon a1 models score higher than the uninterrupted frames the profile agent proposed (a1 86 vs 60), so the RNA-supported models were kept. + ## Open points for the curator -- Gene naming: both homeodomain ORFs are closest to MATa1-type references (phmmer 50 and 47 bits to *C. albicans* MTLA1); the left one is named MATalpha2 after the paper by position only. An HMG-like ORF (470,349-470,951) is not assigned. No MATa2 in the right cassette. -- Both SLA2 ORFs lack a start codon (5-prime partial). The mirrored arm of the palindrome is not recorded. -- The label registry has no entry for `Ascomycota:MATss`; a system code needs your approval before the records are labelled (frozen codes). +- The left MATa1 model is partial (103 aa) and a repeat-rich uninterrupted frame is the alternative; both SLA2 ORFs lack a start codon; the mirrored arm of the palindrome is not recorded; MATa2 also occurs at 527,654-528,454 (mirror) and 502,064-502,864 (central cluster). +- Label codes (approved and frozen 2026-10-30, registry commit on #217): `SacSch` (MATss), `PetFab` (MATpf), `BarCal` (MATbz). - Scope is the species (44080); a heterothallic *Saccharomycopsis* species (*S. lassenensis*) is expected to have one locus with one idiomorph per genome. Other species stay in phylum fallback. ## The other two genera (same session) -`MATpf` (*Petasospora fabianii*, species scope 36022; heterothallic, MATa JOY008 and MATalpha TBRC 4498; AUGUSTUS and miniprot models, no RNA-seq exists) and `MATbz` (*Barnettozyma*, genus scope 599802; one combined PB4207 record, MATalpha2 not modelled) were annotated in `../2026-10-30_petasospora/` and `../2026-10-30_barnettozyma/`. All five candidate records pass `curate-db propose` and `validate` (Slurm job 31446171: `taxonomy_current` true, sequence match not applicable because no record has a protein accession). All are tier 3 (our own models), `needs_review`. +`MATpf` (*Petasospora fabianii*, species scope 36022; heterothallic, MATa JOY008 and MATalpha TBRC 4498; AUGUSTUS and miniprot models, no RNA-seq exists) and `MATbz` (*Barnettozyma*, genus scope 599802; one combined PB4207 record, MATalpha2 a 113 aa partial fragment) were annotated in `../2026-10-30_petasospora/` and `../2026-10-30_barnettozyma/`. All five candidate records pass `curate-db propose` and `validate` (Slurm job 31446171: `taxonomy_current` true, sequence match not applicable because no record has a protein accession). All are tier 3 (our own models), `needs_review`. diff --git a/results/2026-10-30_saccharomycopsis/make_records.py b/results/2026-10-30_saccharomycopsis/make_records.py index e597b390..b2a1820a 100644 --- a/results/2026-10-30_saccharomycopsis/make_records.py +++ b/results/2026-10-30_saccharomycopsis/make_records.py @@ -22,13 +22,15 @@ def protein(exons, strand): # name: (role, strand, exons ascending, expected aa, note) LEFT = { - "MATalpha1": ("core_MAT", "-", [(471020, 471595)], 191, "alpha box (MATalpha_HMGbox 54 bits), SS.1982 ORF p4"), - "MATalpha2": ("core_MAT", "+", [(471961, 472227), (472445, 472489)], 103, "homeodomain (57 bits), partial ORF; 217 bp intron, 198 reads"), + "MATalpha2": ("core_MAT", "+", [(470349, 470951)], 200, "alpha2 profile 73 bits (a1 12); a 92 bp intron (97 reads) gives a 227 aa isoform"), + "MATalpha1": ("core_MAT", "-", [(471020, 471595)], 191, "alpha box (MATalpha_HMGbox 54 bits)"), + "MATa1": ("core_MAT", "+", [(471961, 472227), (472445, 472489)], 103, "a1 profile 86 bits (alpha2 29); partial: 217 bp intron (198 reads) joins it toward SLA2"), "sla2": ("flanking_conserved", "-", [(472708, 473478)], 256, "diverged SLA2 (I_LWEQ 186 bits); a second piece 473,680-474,606 (309 aa) is not recorded"), } RIGHT = { "sla2": ("flanking_conserved", "+", [(533981, 535828)], 615, "SLA2 anchor (I_LWEQ 183 bits), SS.2002.2 ORF p1; mirrored copy 529,992-532,490"), - "MATa1": ("core_MAT", "-", [(536046, 536090), (536296, 536634), (536693, 536944)], 211, "homeodomain (54 bits); introns 205 bp (221 reads) and 58 bp (218 reads)"), + "MATa1": ("core_MAT", "-", [(536046, 536090), (536296, 536634), (536693, 536944)], 211, "a1 profile 85 bits (alpha2 27); introns 205 bp (221 reads) and 58 bp (218 reads)"), + "MATa2": ("core_MAT", "+", [(537379, 538170)], 263, "a2 profile hit, head-to-head with MATa1; ATG to stop, single exon; mirror copy 527,654-528,454 (minus)"), } for nm, genes in (("left", LEFT), ("right", RIGHT)): for g, (role, strand, ex, aa, _) in genes.items(): @@ -60,31 +62,33 @@ def record(rid, idiomorph, spec, orient, note, span): "extended_flank": [], "assembly_accession": "GCA_054953955.1", "assembly_accession_basis": "strain_match_verified", "assembly_location": {"contig": "CM143129.1", "start": lo, "end": hi, "identity": 100.0}} return {"record_id": rid, "record_version": 1, "taxonomy": TAX, "organism": ORG, - "mating_type": {"locus_name": "MATss", "idiomorphs": [idiomorph], "system": "homothallic"}, + "mating_type": {"locus_name": "MATss", "idiomorphs": idiomorph if isinstance(idiomorph, list) else [idiomorph], "system": "homothallic"}, "locus": loc, "genes": genes, "evidence": {"locus_existence": {"tier": 2, "experimental_method": "published description of the SLA2-MAT cassettes and RNA-seq of the active chromosome IV locus (Kriti et al. 2026)", "citations": CIT}, "boundaries": {"tier": 3, "experimental_method": "OUR OWN gene models from the public RNA-seq (PRJNA1364152; StringTie, TransDecoder, phmmer/Pfam) because the authors' GTF is not public; coordinates are ours, the locus span is the paper's", "citations": CIT}, - "idiomorph_assignment": {"tier": 3, "experimental_method": "the paper's assignment of the sub-cluster (alpha-dominant left, palindromic a right); gene names follow it by position; phmmer ranks both homeodomain ORFs closest to MATa1-type references, so MATalpha2 is a hypothesis", "citations": CIT}}, + "idiomorph_assignment": {"tier": 3, "experimental_method": "new MATa1, MATalpha2 and MATa2 profiles (a score difference, supporting evidence: results/2026-10-30_mat_hmms); the sub-clusters are the paper's", "citations": CIT}}, "validation": {"status": "needs_review", "rejection_reason": None, "accession_resolved": False, "accession_resolved_date": None, "accession_resolved_version": None, "sequence_match": {"status": "pass", "per_gene": [], "notes": ""}, "taxonomy_current": None}, - "curation": {"proposed_by": "claude-code-curation-agent", "proposal_dedupe_key": f"41849659|44080|MATss|{idiomorph}", "reviewed_by": None, "reviewed_date": None}, + "curation": {"proposed_by": "claude-code-curation-agent", "proposal_dedupe_key": f"41849659|44080|MATss|{'-'.join(idiomorph) if isinstance(idiomorph, list) else idiomorph}", "reviewed_by": None, "reviewed_date": None}, "model_provenance": None} NOTE_L = ("Left (alpha-dominant) sub-cluster of the active MAT locus on chromosome IV of S. schoenii GDD12 (CM143129.1; the paper's span 470,610-475,000, " "Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC (Dost et al. 2024, PMID 38492364); one of several SLA2-MAT cassettes (one per chromosome plus a palindromic one on chromosome IV). " "GENE MODELS ARE OURS (tier 3): the BRAKER2 GTF the paper cites is not on its GitHub or Zenodo, so the models were rebuilt from the three public paired RNA-seq runs " "(PRJNA1364152; hisat2, StringTie, TransDecoder, phmmer against the curated MAT proteins, Pfam-A); see results/2026-10-29_ss_records/README.md. The library is not stranded; " - "strand is the ORF's. MATalpha1 is single-exon, minus strand, 191 aa. The homeodomain ORF (named MATalpha2 after the paper) is partial (103 aa, two exons with a 217 bp intron of 198 reads that " - "joins it toward SLA2, the paper's MAT-SLA2 splice junction); phmmer ranks it closest to MATa1-type references (50 bits to C. albicans MTLA1), so the name is a hypothesis. " - "SLA2 here is a diverged copy: 256 aa (472,708-473,478, minus), 5-prime partial (the ORF has no start codon); a second SLA2 piece (473,680-474,606, 309 aa) is not recorded. An HMG-like ORF at 470,349-470,951 (200 aa, 44 bits to " - "S. cerevisiae MATA2, no Pfam hit) is not assigned to a gene. GENETIC CODE: NCBI table 12 (CUG = Ser) for the species; proteins are translated with it.") + "strand is the ORF's. GENE NAMES come from new MATa1, MATalpha2 and MATa2 profiles (results/2026-10-30_mat_hmms; supporting evidence, a score difference, not a gate): the ORF at 470,349-470,951 " + "(200 aa, single exon, 227 aa with a 92 bp intron of 97 reads) is MATalpha2 (alpha2 profile 73 bits, a1 12) and not an HMG-box MATa2; the homeodomain at 471,961-472,489 is MATa1 (a1 86, alpha2 29), " + "so the cluster holds both an alpha pair and an a1 beside SLA2 (the paper's MATa1-SLA2 junction). MATalpha1 is single-exon, minus strand, 191 aa. The MATa1 model is partial (103 aa, two exons with a 217 bp intron of " + "198 reads that joins it toward SLA2); the uninterrupted frame through the intron scores lower (a1 60) and runs through a low-complexity repeat. " + "SLA2 is a diverged copy: 256 aa (472,708-473,478, minus), 5-prime partial (no start codon); a second SLA2 piece (473,680-474,606, 309 aa) is not recorded. GENETIC CODE: NCBI table 12 (CUG = Ser).") NOTE_R = ("Right (palindromic) cassette of the active MAT locus on chromosome IV of S. schoenii GDD12 (CM143129.1; the paper's span 528,044-537,777; an SLA2 anchor between mirrored MAT arrays, " - "Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC (Dost et al. 2024, PMID 38492364). The record covers the arm that RNA-seq resolves: SLA2 533,981-535,828 (plus, 615 aa, 5-prime partial: no start codon in the ORF) and the homeodomain gene " - "named MATa1 after the paper at 536,046-536,944 (minus, 211 aa; introns of 205 bp, 221 reads, and 58 bp, 218 reads, the conserved 58 bp junction the paper reports at 536,635). The mirrored arm " - "(SLA2 529,992-532,490; homeodomain ORF 529,258-529,749, 163 aa) is not recorded: short reads cannot separate the two inverted copies, and the paper's mirrored junction at 529,128 is not seen. No HMG-box " - "(MATa2) ORF passed the filters. GENE MODELS ARE OURS (tier 3), see results/2026-10-29_ss_records/README.md. The library is not stranded. GENETIC CODE: NCBI table 12 (CUG = Ser).") -ra = record("44080_gdd12_MATss_MATalpha", "MATalpha", LEFT, "MATalpha1->MATalpha2->sla2", NOTE_L, None) -rb = record("44080_gdd12_MATss_MATa", "MATa", RIGHT, "sla2->MATa1", NOTE_R, None) + "Kriti et al. G3 2026, PMID 41849659). HOMOTHALLIC (Dost et al. 2024, PMID 38492364). The record covers the arm that RNA-seq resolves: SLA2 533,981-535,828 (plus, 615 aa, 5-prime partial: no start codon in the ORF), " + "MATa1 at 536,046-536,944 (minus, 211 aa; a1 profile 85 bits, alpha2 27; introns of 205 bp, 221 reads, and 58 bp, 218 reads, the conserved 58 bp junction the paper reports at 536,635) and MATa2 at 537,379-538,170 " + "(plus, 263 aa, ATG to stop, single exon, head-to-head with MATa1; a2 profile hit, results/2026-10-30_mat_hmms). The a2 profile finds only three a2-like ORFs in the whole genome, all on chromosome IV: this one, its mirror " + "at 527,654-528,454 (minus) and one at 502,064-502,864 at the central cluster. The mirrored arm (SLA2 529,992-532,490; MATa1 529,186-529,746) is not recorded: short reads cannot separate the two inverted copies, " + "and the paper's mirrored junction at 529,128 is not seen. GENE MODELS ARE OURS (tier 3), see results/2026-10-29_ss_records/README.md. The library is not stranded. GENETIC CODE: NCBI table 12 (CUG = Ser).") +ra = record("44080_gdd12_MATss_MATa-MATalpha", ["MATa", "MATalpha"], LEFT, "MATalpha2->MATalpha1->MATa1->sla2", NOTE_L, None) +rb = record("44080_gdd12_MATss_MATa", "MATa", RIGHT, "sla2->MATa1->MATa2", NOTE_R, None) for r in (ra, rb): open(f"{OUT}{r['record_id']}.yaml", "w").write(yaml.safe_dump(r, sort_keys=False)) print(r["record_id"], [(g["name"], g["start"], g["end"], g["strand"], len(g["exons"])) for g in r["genes"]]) diff --git a/results/2026-10-30_saccharomycopsis/validate.slurm b/results/2026-10-30_saccharomycopsis/validate.slurm index 61d958f8..f466bc6d 100644 --- a/results/2026-10-30_saccharomycopsis/validate.slurm +++ b/results/2026-10-30_saccharomycopsis/validate.slurm @@ -5,7 +5,7 @@ R=/bigdata/stajichlab/jstajich/projects/MATPredict T=$R/results/2026-10-30_saccharomycopsis/tree export PATH="$R/.pixi/envs/default/bin:$PATH" cd $T -for r in 44080_gdd12_MATss_MATalpha 44080_gdd12_MATss_MATa 36022_joy008_MATpf_MATa 36022_tbrc-4498_MATpf_MATalpha 36038_pb4207_MATbz_MATa-MATalpha; do +for r in 44080_gdd12_MATss_MATa-MATalpha 44080_gdd12_MATss_MATa 36022_joy008_MATpf_MATa 36022_tbrc-4498_MATpf_MATalpha 36038_pb4207_MATbz_MATa-MATalpha; do PYTHONPATH="$T/src" MATPREDICT_DB_ROOT="$T/db" python -m MATPredict curate-db validate --phylum Ascomycota --record-id $r > $T/$r.validate.json grep -n "taxonomy_current\|taxonomy_error\|sequence_match\|status" -A1 $T/$r.validate.json | head -12 done diff --git a/tests/detect/test_matss_family.py b/tests/detect/test_matss_family.py index a4216a83..7f025c11 100644 --- a/tests/detect/test_matss_family.py +++ b/tests/detect/test_matss_family.py @@ -23,7 +23,7 @@ def test_matss_declaration(): def test_matss_candidates_are_tier_3_models_with_table_12(): - for rid in ("44080_gdd12_MATss_MATalpha", "44080_gdd12_MATss_MATa"): + for rid in ("44080_gdd12_MATss_MATa-MATalpha", "44080_gdd12_MATss_MATa"): rec = yaml.safe_load((DB / "candidates" / "Ascomycota" / rid / "metadata.yaml").read_text()) assert rec["mating_type"]["locus_name"] == "MATss" and rec["mating_type"]["system"] == "homothallic" assert rec["evidence"]["boundaries"]["tier"] == 3