diff --git a/src/Microsoft.ComponentDetection.Detectors/rust/Parsers/RustCliParser.cs b/src/Microsoft.ComponentDetection.Detectors/rust/Parsers/RustCliParser.cs
index 11ac3c101..f1a4f3271 100644
--- a/src/Microsoft.ComponentDetection.Detectors/rust/Parsers/RustCliParser.cs
+++ b/src/Microsoft.ComponentDetection.Detectors/rust/Parsers/RustCliParser.cs
@@ -70,6 +70,10 @@ public async Task<ParseResult> ParseAsync(
                 return result;
             }
 
+            var manifestPath = componentStream.Location.Contains(' ') && !componentStream.Location.StartsWith('"')
+                ? $"\"{componentStream.Location}\""
+                : componentStream.Location;
+
             var cliResult = await this.cliService.ExecuteCommandAsync(
                 command: "cargo",
                 additionalCandidateCommands: null,
@@ -77,7 +81,7 @@ public async Task<ParseResult> ParseAsync(
                 cancellationToken: cancellationToken,
                 "metadata",
                 "--manifest-path",
-                componentStream.Location,
+                manifestPath,
                 "--format-version=1",
                 "--locked");
 
diff --git a/src/Microsoft.ComponentDetection.Detectors/rust/RustMetadataContextBuilder.cs b/src/Microsoft.ComponentDetection.Detectors/rust/RustMetadataContextBuilder.cs
index c728e227e..55229734f 100644
--- a/src/Microsoft.ComponentDetection.Detectors/rust/RustMetadataContextBuilder.cs
+++ b/src/Microsoft.ComponentDetection.Detectors/rust/RustMetadataContextBuilder.cs
@@ -185,6 +185,10 @@ private async Task<CargoMetadata> RunCargoMetadataAsync(string manifestPath, Can
             return null;
         }
 
+        var manifestPathArg = manifestPath.Contains(' ') && !manifestPath.StartsWith('"')
+            ? $"\"{manifestPath}\""
+            : manifestPath;
+
         var res = await this.cliService.ExecuteCommandAsync(
             "cargo",
             additionalCandidateCommands: null,
@@ -192,7 +196,7 @@ private async Task<CargoMetadata> RunCargoMetadataAsync(string manifestPath, Can
             cancellationToken: token,
             "metadata",
             "--manifest-path",
-            manifestPath,
+            manifestPathArg,
             "--format-version=1",
             "--locked");
 
diff --git a/test/Microsoft.ComponentDetection.Detectors.Tests/RustCliParserTests.cs b/test/Microsoft.ComponentDetection.Detectors.Tests/RustCliParserTests.cs
index 1fb1b7b54..c9e3ac017 100644
--- a/test/Microsoft.ComponentDetection.Detectors.Tests/RustCliParserTests.cs
+++ b/test/Microsoft.ComponentDetection.Detectors.Tests/RustCliParserTests.cs
@@ -1136,6 +1136,37 @@ public async Task VirtualManifest_MultipleRootNodes_AllProcessed()
         distinctNames.Should().Contain("pkgB");
     }
 
+    [TestMethod]
+    public async Task ParseAsync_QuotesManifestPathWhenPathContainsSpaces()
+    {
+        var tomlPath = "C:/repo with space/AppControl Manager/Cargo.toml";
+        var quotedTomlPath = $"\"{tomlPath}\"";
+
+        var json = """
+        {
+          "packages": [
+            { "name": "my-pkg", "version": "1.0.0", "id": "my-pkg 1.0.0 (path+file:///C:/repo)", "dependencies": [], "source": null }
+          ],
+          "workspace_members": [ "my-pkg 1.0.0 (path+file:///C:/repo)" ],
+          "resolve": {
+            "nodes": [
+              { "id": "my-pkg 1.0.0 (path+file:///C:/repo)", "deps": [] }
+            ]
+          }
+        }
+        """;
+
+        this.cli.Setup(c => c.CanCommandBeLocatedAsync("cargo", null)).ReturnsAsync(true);
+        this.cli.Setup(c => c.ExecuteCommandAsync("cargo", null, null, It.IsAny<CancellationToken>(), "metadata", "--manifest-path", quotedTomlPath, "--format-version=1", "--locked"))
+            .ReturnsAsync(new CommandLineExecutionResult { ExitCode = 0, StdOut = json });
+
+        var recorder = new Mock<ISingleFileComponentRecorder>(MockBehavior.Loose);
+        var result = await this.parser.ParseAsync(MakeTomlStream(tomlPath), recorder.Object);
+
+        result.Success.Should().BeTrue();
+        this.cli.Verify(c => c.ExecuteCommandAsync("cargo", null, null, It.IsAny<CancellationToken>(), "metadata", "--manifest-path", quotedTomlPath, "--format-version=1", "--locked"), Times.Once());
+    }
+
     private async Task<ParseResult> InvokeProcessMetadataAsync(string manifestLocation, ISingleFileComponentRecorder fallbackRecorder, CargoMetadata metadata) =>
         await this.parser.ParseFromMetadataAsync(
             new ComponentStream { Location = manifestLocation, Pattern = "Cargo.toml", Stream = new MemoryStream([]) },
diff --git a/test/Microsoft.ComponentDetection.Detectors.Tests/RustMetadataContextBuilderTests.cs b/test/Microsoft.ComponentDetection.Detectors.Tests/RustMetadataContextBuilderTests.cs
index 5e70e9b80..9e135cef8 100644
--- a/test/Microsoft.ComponentDetection.Detectors.Tests/RustMetadataContextBuilderTests.cs
+++ b/test/Microsoft.ComponentDetection.Detectors.Tests/RustMetadataContextBuilderTests.cs
@@ -180,6 +180,57 @@ public async Task BuildPackageOwnershipMapAsync_CargoMetadataFails_AddsToFailedM
         result.ManifestToMetadata.Should().BeEmpty();
     }
 
+    [TestMethod]
+    public async Task BuildPackageOwnershipMapAsync_QuotesManifestPathWhenPathContainsSpaces()
+    {
+        var tomlPath = "C:/repo with space/AppControl Manager/Cargo.toml";
+        var quotedTomlPath = $"\"{tomlPath}\"";
+
+        var json = """
+        {
+          "packages": [
+            { "name": "my-pkg", "version": "1.0.0", "id": "my-pkg 1.0.0 (path+file:///C:/repo)", "dependencies": [], "source": null }
+          ],
+          "workspace_members": [ "my-pkg 1.0.0 (path+file:///C:/repo)" ],
+          "resolve": {
+            "nodes": [
+              { "id": "my-pkg 1.0.0 (path+file:///C:/repo)", "deps": [] }
+            ]
+          }
+        }
+        """;
+
+        this.envVarService.Setup(e => e.IsEnvironmentVariableValueTrue("DisableRustCliScan")).Returns(false);
+        this.cliService.Setup(c => c.CanCommandBeLocatedAsync("cargo", null)).ReturnsAsync(true);
+        this.cliService.Setup(c => c.ExecuteCommandAsync(
+                "cargo",
+                null,
+                null,
+                It.IsAny<CancellationToken>(),
+                "metadata",
+                "--manifest-path",
+                quotedTomlPath,
+                "--format-version=1",
+                "--locked"))
+            .ReturnsAsync(new CommandLineExecutionResult { ExitCode = 0, StdOut = json });
+
+        var result = await this.builder.BuildPackageOwnershipMapAsync([tomlPath]);
+
+        result.FailedManifests.Should().BeEmpty();
+        this.cliService.Verify(
+            c => c.ExecuteCommandAsync(
+                "cargo",
+                null,
+                null,
+                It.IsAny<CancellationToken>(),
+                "metadata",
+                "--manifest-path",
+                quotedTomlPath,
+                "--format-version=1",
+                "--locked"),
+            Times.Once());
+    }
+
     [TestMethod]
     public async Task BuildPackageOwnershipMapAsync_SimpleDependency_BuildsOwnershipMap()
     {