diff --git a/packages/docusaurus-utils/src/__tests__/markdownUtils.test.ts b/packages/docusaurus-utils/src/__tests__/markdownUtils.test.ts
index 34648991ba35..646604f1a1fb 100644
--- a/packages/docusaurus-utils/src/__tests__/markdownUtils.test.ts
+++ b/packages/docusaurus-utils/src/__tests__/markdownUtils.test.ts
@@ -438,6 +438,113 @@ Lorem Ipsum
     });
   });
 
+  it('preserves trailing # in title without preceding space (e.g. C# or F#)', () => {
+    expect(
+      parseMarkdownContentTitle(dedent`
+        # Learn C#
+
+        Lorem Ipsum
+      `),
+    ).toEqual({
+      content: dedent`
+        # Learn C#
+
+        Lorem Ipsum
+      `,
+      contentTitle: 'Learn C#',
+    });
+
+    expect(
+      parseMarkdownContentTitle(dedent`
+        # F#
+
+        Lorem Ipsum
+      `),
+    ).toEqual({
+      content: dedent`
+        # F#
+
+        Lorem Ipsum
+      `,
+      contentTitle: 'F#',
+    });
+
+    expect(
+      parseMarkdownContentTitle(dedent`
+        # Learn C# {#learn-csharp}
+
+        Lorem Ipsum
+      `),
+    ).toEqual({
+      content: dedent`
+        # Learn C# {#learn-csharp}
+
+        Lorem Ipsum
+      `,
+      contentTitle: 'Learn C#',
+    });
+
+    expect(
+      parseMarkdownContentTitle(dedent`
+        # Learn C# #
+
+        Lorem Ipsum
+      `),
+    ).toEqual({
+      content: dedent`
+        # Learn C# #
+
+        Lorem Ipsum
+      `,
+      contentTitle: 'Learn C#',
+    });
+
+    expect(
+      parseMarkdownContentTitle(dedent`
+        # Learn C# ## {#learn-csharp}
+
+        Lorem Ipsum
+      `),
+    ).toEqual({
+      content: dedent`
+        # Learn C# ## {#learn-csharp}
+
+        Lorem Ipsum
+      `,
+      contentTitle: 'Learn C# ##',
+    });
+
+    expect(
+      parseMarkdownContentTitle(dedent`
+        # #
+
+        Lorem Ipsum
+      `),
+    ).toEqual({
+      content: dedent`
+        # #
+
+        Lorem Ipsum
+      `,
+      contentTitle: '',
+    });
+
+    expect(
+      parseMarkdownContentTitle(dedent`
+        # ##
+
+        Lorem Ipsum
+      `),
+    ).toEqual({
+      content: dedent`
+        # ##
+
+        Lorem Ipsum
+      `,
+      contentTitle: '',
+    });
+  });
+
   it('parses markdown h1 title at the top followed by h2 title', () => {
     const markdown = dedent`
 
diff --git a/packages/docusaurus-utils/src/markdownUtils.ts b/packages/docusaurus-utils/src/markdownUtils.ts
index 76f824f33ebd..be67c4e305e6 100644
--- a/packages/docusaurus-utils/src/markdownUtils.ts
+++ b/packages/docusaurus-utils/src/markdownUtils.ts
@@ -334,7 +334,7 @@ export function parseMarkdownContentTitle(
       contentTitle: toTextContentTitle(
         regularTitleMatch
           .groups!.title!.trim()
-          .replace(/\s*(?:\{#*[\w-]+\}|#+)$/, ''),
+          .replace(/(?:\s*\{#*[\w-]+\}|(?:^|\s+)#+)$/, ''),
       ).trim(),
     };
   }